Mixed fruit powder, polypeptide, probiotic composition and composite beverage

Abstract

Active components of the mixed fruit powder, polypeptide and probiotic composition comprise the following components in parts by weight: 45-60 parts of mixed fruit powder, 30-50 parts of polypeptide and 5-25 parts of probiotics, wherein the mixed fruit powder comprises a mixture of two or more of cherry fruit powder, roxburgh rose fruit powder, cranberry fruit powder, pomegranate fruit powder, sea buckthorn fruit powder and mulberry fruit powder; the polypeptide comprises one or more of oyster peptide, sea cucumber peptide or krill peptide; the probiotics comprise one or more of lactobacillus paracasei JLPF-131, lactobacillus acidophilus JYLA-191 or bifidobacterium lactis JYBR-390, and the probiotics comprise one or more of lactobacillus paracasei JLPF-131, lactobacillus acidophilus JYLA-191 and bifidobacterium When the probiotic food or health-care product is developed, the requirements of diarrhea patients are considered, the use of prebiotics is avoided as much as possible, and the reproduction of the probiotics can also be ensured.

Description

Technical Field

[0001] The present invention relates to the field of food and health products, and in particular to a mixed fruit powder, polypeptide, probiotic composition and a compound drink. Background Art

[0002] Probiotics are a type of active microorganisms that are beneficial to the host by colonizing in the human body and changing the composition of the flora in a certain part of the host. They have the functions of promoting the digestion and absorption of food by the human body, enhancing human immunity, preventing constipation and diarrhea, inhibiting the growth of harmful intestinal bacteria, and decomposing carcinogens. The reproduction and growth of probiotics require a certain proportion of carbon source, nitrogen source and trace elements. Prebiotics are often added to probiotic foods as a dietary supplement to promote the growth of probiotics.

[0003] Prebiotics are functional oligosaccharides, and common ones include fructooligosaccharides, soybean oligosaccharides, galacto-oligosaccharides, isomaltooligosaccharides, xylooligosaccharides, manno-oligosaccharides, chito-oligosaccharides, lactitol, isomaltulose, etc. Prebiotics cannot be decomposed, absorbed, and utilized by the human body. After reaching the colon through the digestive tract, some can be decomposed and utilized by colon flora, which promotes the growth of colon flora and plays an important role in improving intestinal microecology and promoting the metabolism of lipids, proteins, and minerals.

[0004] However, since intestinal enzymes cannot hydrolyze prebiotics, in the case of diarrhea-type bowel syndrome, prebiotics participate in the fermentation of intestinal microorganisms, which will stimulate the colon to produce gas and cause the patient's abdominal distension to increase, which brings trouble to the development of probiotic foods or health products. Summary of the invention

[0005] In view of the above situation, in order to solve the problems existing in the above technology, the present invention proposes a mixed fruit powder, polypeptide, probiotic composition and compound beverage. When developing probiotic food or health products, the needs of diarrhea patients are considered, and the use of prebiotics is avoided as much as possible, while the reproduction of probiotics can be guaranteed.

[0006] On the one hand, the present invention provides a mixed fruit powder, polypeptide, probiotic composition, which has a simple formula and includes a suitable amount of mixed fruit powder, polypeptide and probiotics. The mixed fruit powder, polypeptide, probiotic composition of the present invention uses probiotics as the main functional component, uses mixed fruit powder to provide carbon source and trace elements for probiotics, and uses polypeptide to provide nitrogen source for probiotics, effectively avoids the use of prebiotics, and can ensure good probiotic activity and reproduction, which is more suitable for diarrhea patients and broadens the scope of use of the composition.

[0007] A mixed fruit powder, polypeptide, and probiotic composition, wherein the active ingredients include the following components in parts by weight: 45-60 parts of mixed fruit powder, 30-50 parts of polypeptide, and 5-25 parts of probiotics; wherein the mixed fruit powder includes a mixture of two or more of cherry fruit powder, roxburghii fruit powder, cranberry fruit powder, pomegranate fruit powder, sea buckthorn fruit powder, and mulberry fruit powder; the polypeptide includes one or more of oyster peptide, sea cucumber peptide, or krill peptide; and the probiotics include one or more of Lactobacillus paracasei JLPF-131, Lactobacillus acidophilus JYLA-191, or Bifidobacterium lactis JYBR-390.

[0008] Preferably, the mixed fruit powder comprises the following components in parts by weight: 20-40 parts of seabuckthorn fruit powder and 5-10 parts of mulberry fruit powder; or 20-40 parts of seabuckthorn fruit powder and 5-10 parts of roxburghii fruit powder.

[0009] Preferably, the mixed fruit powder comprises the following components in parts by weight: 20-40 parts of sea buckthorn fruit powder, 5-10 parts of mulberry fruit powder, 5-10 parts of cherry fruit powder and 5-10 parts of cranberry fruit powder.

[0010] Preferably, the mixed fruit powder comprises the following components in parts by weight: 20-40 parts of sea buckthorn fruit powder, 5-10 parts of mulberry fruit powder, 5-10 parts of roxburghii fruit powder and 5-10 parts of pomegranate fruit powder.

[0011] Preferably, the mixed fruit powder comprises the following components in parts by weight: 20-40 parts of sea buckthorn fruit powder, 1-5 parts of mulberry fruit powder, 1-5 parts of cherry fruit powder, 1-5 parts of cranberry fruit powder, 1-5 parts of roxburghii fruit powder and 1-5 parts of pomegranate fruit powder.

[0012] Preferably, the polypeptide comprises the following components in parts by weight: 10-15 parts of oyster peptide, 10-15 parts of sea cucumber peptide and 10-15 parts of krill peptide.

[0013] Preferably, the probiotics include the following components in parts by weight: 5 to 10 parts by weight of Lactobacillus paracasei JLPF-131, 5 to 10 parts by weight of Lactobacillus acidophilus JYLA-191 and 5 to 10 parts by weight of Bifidobacterium lactis JYBR-390.

[0014] Preferably, it also includes one or more of polygonatum powder, raspberry powder and wolfberry powder.

[0015] Preferably, the following components are included in parts by weight: 1-5 parts of polygonatum powder, 1-5 parts of raspberry powder and 1-5 parts of wolfberry powder.

[0016] The second aspect of the present invention is to provide a mixed fruit powder, polypeptide, probiotic compound beverage, which contains the mixed fruit powder, polypeptide, probiotic composition, and the mixed fruit powder, polypeptide, probiotic compound beverage is in liquid form.

[0017] A composite drink using a mixed fruit powder, polypeptide and probiotic composition comprises the following raw materials in weight percentage: 75% to 85% water and 15% to 25% of the mixed fruit powder, polypeptide and probiotic composition, the sum of the weight percentages of the water and the mixed fruit powder, polypeptide and probiotic composition being 100%.

[0018] The third aspect of the present invention is to provide a method for preparing a mixed fruit powder, polypeptide, and probiotic compound beverage, comprising the following steps:

[0019] Mixing a plurality of fruit powders to prepare a mixed fruit powder;

[0020] Mix the mixed fruit powder, peptides and probiotics according to the proportion;

[0021] Add the prepared mixed fruit powder, peptides and probiotics into the liquid and stir to dissolve.

[0022] According to the mixed fruit powder, polypeptide, probiotic composition and compound beverage of the present invention, preferably, the cherry fruit powder is acerola fruit powder.

[0023] After adopting the technology proposed by the present invention, the mixed fruit powder, polypeptide, probiotic composition and compound beverage according to the embodiments of the present invention have the following beneficial effects.

[0024] 1) The mixed fruit powder, polypeptide, probiotic composition and compound beverage according to the present invention use probiotics as the main functional components, use the mixed fruit powder to provide a carbon source and trace elements for the probiotics, and use the polypeptide to provide a nitrogen source for the probiotics, thereby effectively avoiding the use of prebiotics and ensuring good probiotic activity and reproduction, being more suitable for diarrhea patients and broadening the scope of use of the composition or compound beverage.

[0025] 2) The mixed fruit powder, polypeptide, probiotic composition and compound beverage according to the present invention are added with mixed fruit powder, including one or more of cherry fruit powder, roxburghii fruit powder, cranberry fruit powder, pomegranate fruit powder, sea buckthorn fruit powder and mulberry fruit powder, which can not only provide carbon source and trace elements for probiotics, but also contain rich vitamin C, fruit acid, polysaccharides, anthocyanins and polyphenols, providing a variety of beneficial substances for the human body.

[0026] 3) According to the mixed fruit powder, polypeptide, probiotic composition and compound beverage of the present invention, polypeptides are added, including one or more of oyster peptides, sea cucumber peptides and krill peptides, wherein oyster peptides are a small molecule oligopeptide, which completely retains the original vitamins, trace elements and taurine and other nutritional components of oysters, so that oysters rich in nucleic acid are absorbed faster than single amino acids or proteins after being ingested by the human body, and are more easily absorbed by the body, and have more important biological activity in human metabolism, and can effectively increase the serum testosterone level of men; sea cucumber peptides are extracts of sea cucumbers, which have the effect of regulating the functions of the human body, It can repair damaged cells and enhance the body's immunity, and has a series of effects such as anti-fatigue and anti-aging. Krill peptide, made from Antarctic krill, can significantly increase the survival time, oxygen consumption and swimming time of mice, increase LDH, liver glycogen content, LA clearance rate and liver and spleen weight; reduce the content of LA and BUA, and can increase the proliferation rate of mouse spleen lymphocytes, delayed-type hypersensitivity, antibody-producing cell number, serum hemolysin level, phagocyte carbon clearance ability and NK cell activity, and has the effects of significantly improving anti-fatigue, hypoxia resistance, anti-aging and improving immunity. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 A data comparison table of sensory perception, flavor and viable bacteria count of beverages in various embodiments of the present invention and comparative examples is shown. DETAILED DESCRIPTION

[0028] The various preferred embodiments of the present invention will be described below with reference to the accompanying drawings. The following description with reference to the accompanying drawings is provided to assist in the understanding of the exemplary embodiments of the present invention as defined by the claims and their equivalents. It includes various specific details to assist in understanding, but they are to be viewed as exemplary only. Therefore, those skilled in the art will recognize that various changes and modifications may be made to the embodiments described herein without departing from the scope and spirit of the present invention. Moreover, in order to make the specification more clear and concise, detailed descriptions of functions and configurations well known in the art will be omitted.

[0029] The following are some specific embodiments.

[0030] For experimental parameters not specified in the following specific embodiments, reference is made to the instructions given in the present application document, and reference may also be made to experimental manuals in the art or other experimental methods known in the art, or to experimental conditions recommended by manufacturers.

[0031] The raw materials and reagents involved in the following specific examples can be obtained from commercial sources, or can be prepared by those skilled in the art according to known methods.

[0032] Example 1

[0033] By weight, 45 g of mixed fruit powder was weighed, the mixed fruit powder including 27 g of sea buckthorn fruit powder and 18 g of roxburghii fruit powder; 35 g of oyster peptide and 20 g of Lactobacillus paracasei JLPF-131 were weighed; the mixture was added into a blender and mixed for 30 minutes to obtain a mixed powder; the mixed powder was sieved and added into 800 g of pure water to obtain a mixed fruit powder, polypeptide and probiotic beverage.

[0034] Example 2

[0035] The mixed fruit powder, polypeptide and probiotic beverage was prepared in a manner substantially the same as in Example 1, except that the mixed fruit powder included 27 g of the sea buckthorn fruit powder and 18 g of the cranberry fruit powder; the remaining parameters, operations and preparation methods were the same as in Example 1.

[0036] Example 3

[0037] The mixed fruit powder, polypeptide and probiotic beverage were prepared in a manner substantially the same as in Example 1, except that the mixed fruit powder included 27 g of the sea buckthorn fruit powder and 18 g of the roxburghii fruit powder; the remaining parameters, operations and preparation methods were the same as in Example 1.

[0038] Example 4

[0039] The mixed fruit powder, polypeptide and probiotic beverage were prepared in a manner substantially the same as in Example 1, except that the mixed fruit powder included 40 g of the sea buckthorn fruit powder, 5 g of the mulberry fruit powder, 5 g of the cherry fruit powder and 5 g of the cranberry fruit powder; the remaining parameters, operations and preparation methods were the same as in Example 1.

[0040] Example 5

[0041] The mixed fruit powder, polypeptide and probiotic beverage were prepared in a manner substantially the same as in Example 1, except that the mixed fruit powder included 40 g of the sea buckthorn fruit powder, 5 g of the mulberry fruit powder, 5 g of the cherry fruit powder and 5 g of the cranberry fruit powder; the remaining parameters, operations and preparation methods were the same as in Example 1.

[0042] Example 6

[0043] The mixed fruit powder, polypeptide and probiotic beverage were prepared in a manner substantially the same as in Example 1, except that the mixed fruit powder included 40 g of the sea buckthorn fruit powder, 5 g of the mulberry fruit powder, 5 g of the roxburghii fruit powder and 5 g of the pomegranate fruit powder; the remaining parameters, operations and preparation methods were the same as in Example 1.

[0044] Example 7

[0045] The mixed fruit powder, polypeptide and probiotic beverage was prepared in a manner substantially the same as in Example 1, except that 10 g of oyster peptide, 10 g of sea cucumber peptide and 15 g of krill peptide were weighed to replace the oyster peptide; the remaining parameters, operations and preparation methods were the same as in Example 1.

[0046] Example 8

[0047] The mixed fruit powder, polypeptide and probiotic beverage were prepared in substantially the same manner as in Example 1, except that 20 g of Lactobacillus acidophilus JYLA-191 was weighed instead of Lactobacillus paracasei JLPF-131; the remaining parameters, operations and preparation methods were the same as in Example 1.

[0048] Example 9

[0049] The mixed fruit powder, polypeptide and probiotic beverage were prepared in substantially the same manner as in Example 1, except that 20 g of Bifidobacterium lactis JYBR-390 was weighed instead of Lactobacillus paracasei JLPF-131; the remaining parameters, operations and preparation methods were the same as in Example 1.

[0050] Example 9

[0051] Mixed fruit powder, polypeptide and probiotic drink were prepared in a manner substantially the same as in Example 1, except that 6 g of Lactobacillus paracasei JLPF-131, 6 g of Lactobacillus acidophilus JYLA-191 and 8 g of Bifidobacterium lactis JYBR-390 were weighed to replace Lactobacillus paracasei JLPF-131; and the remaining parameters, operation and preparation method were the same as in Example 1.

[0052] Example 10

[0053] A mixed fruit powder, polypeptide, and probiotic beverage was prepared in substantially the same manner as in Example 1, except that 3 g of polygonatum powder, 3 g of raspberry powder, and 3 g of wolfberry powder were added; the remaining parameters, operations, and preparation methods were the same as in Example 1.

[0054] In the technical scheme and some embodiments of the present invention, the mixed fruit powder includes a mixture of two or more of cherry fruit powder, roxburghii fruit powder, cranberry fruit powder, pomegranate fruit powder, sea buckthorn fruit powder and mulberry fruit powder.

[0055] Preferably, the cherry fruit powder is acerola fruit powder. Vitamin C is an important component that is indispensable for maintaining human life, preventing scurvy, improving human resistance, and has certain effects on beauty and cancer. Acerola cherry is rich in vitamin C, with a vitamin C content of 1677 mg per 100 grams of fruit, second only to kakadu plum and camu, 31 times that of lemon, 27 times that of strawberry, 18 times that of kiwi, and 7 times that of guava. It can provide vitamins A, E, P, vitamin B group, protein and a variety of mineral trace elements necessary for the human body, as well as its unique anti-aging factor-superoxide dismutase (SOD). Pick fresh cherry fruits, dry the fresh cherry fruits, grind them into powder, sieve them, and prepare cherry fruit powder, so the cherry fruit powder has anti-aging, antioxidant and whitening effects.

[0056] The main nutrients of roxburghii are as follows: ① Carbohydrates: The total amount of carbohydrates in roxburghii fruit is about 13%, and the main components are fiber, sucrose, glucose, fructose, etc. Among them, the content of cellulose is 4%-7%, sucrose is 1.4%-3.5%, glucose is 0.1%-0.9%, and fructose is 0.6%-1.2%; ② Protein and amino acids: protein is 0.6%-0.7%; amino acids include 18 kinds of amino acids such as aspartic acid, threonine, valine, methionine, phenylalanine, lysine, histidine and arginine. Among them, 8 kinds of amino acids are essential amino acids for human body, and 2 kinds of amino acids are semi-essential amino acids; ③ Vitamins and tannins: mainly vitamin C, vitamin P, vitamin B group (vitamin B1, B2, etc.), vitamin E, folic acid and carotene and other vitamins and tannic acid, among which the content of vitamin C is relatively high. Nutritionists Luo Dengyi and Professor Song Puju found that every 100 grams of sea buckthorn pulp contains 2075mg to 2725mg of vitamin C, which is 800 times that of apples, 400 times that of bananas, 100 times that of red oranges, 22 times that of tomatoes, and 10 times that of kiwis. It is called the "King of Vitamin C" and is the top of all fruits and vegetables; ④ Trace elements: The main springs are iron (Fe), zinc (Zn), strontium (Sr), selenium (Se), rubidium (Rb), manganese (Mn), copper (Cu), cobalt (Co), nickel (Ni), fluorine (F), aluminum (A1), vanadium (V), silicon (Si), etc. Among them, Fe, Zn, Se, Mn, Cu, Co, Ni, F, V and Si are essential trace elements for the human body; ⑤ Enzymes: mainly superoxide dismutase (SOD) and peroxidase. Fresh roxburgh fruits are picked, dried, ground into powder, sieved, and prepared into roxburgh fruit powder. Therefore, the roxburgh fruit powder can not only provide carbon source and trace elements for probiotics, but also has the functions of assisting in regulating immune function and delaying aging.

[0057] Cranberries contain proanthocyanidins, ellagic acid, phenolic acid, resveratrol and other substances, which have antibacterial effects. Many studies have shown that cranberries can inhibit the infection of Helicobacter pylori and have a good effect on preventing gastric ulcers, gastric cancer and duodenal ulcers; the flavonoids in cranberries have antioxidant functions in in vivo and in vitro tests, especially flavonols and proanthocyanidins have a preventive effect on atherosclerosis; fresh cranberries are picked, dried, ground into powder, sieved, and cranberry powder is prepared. Therefore, cranberry powder is beneficial for preventing gastric ulcers, gastric cancer, duodenal ulcers, anti-oxidation and preventing arteriosclerosis.

[0058] Pomegranate contains 17% carbohydrates, 79% water, 13%-17% sugar, vitamin C, and minerals such as calcium, phosphorus, and potassium. It also contains anthocyanins and pomegranate polyphenols, which can resist oxidation and protect the eyes. Pomegranate fruit powder is prepared by picking fresh pomegranate fruits, drying them, grinding them into powder, sieving them, and thus pomegranate fruit powder can not only provide carbon water and trace elements for probiotics, but also resist oxidation and protect eyesight.

[0059] The main role of sea buckthorn in beauty is sea buckthorn oil, which contains a large amount of vitamin E, vitamin A, flavonoids and SOD active ingredients that can effectively prevent free radicals to achieve anti-aging effects; fresh sea buckthorn fruits are picked, dried, ground into powder, sieved, and sea buckthorn fruit powder is prepared to make it anti-aging.

[0060] Mulberries are rich in protein, essential amino acids, total sugar, free acid, crude fiber, vitamins, minerals and other functional ingredients, and have high nutritional value. The vitamins they contain include VA, VC, VB, VE and carotene. Mulberries are rich in mineral elements, and calcium, phosphorus, iron, copper, zinc and other elements occupy a certain proportion in mulberries. Among them, selenium is relatively rich, with a content of 4.6μg / 100g; mulberries are also found to contain anti-aging trace elements molybdenum and strontium. Pick fresh mulberries, dry them, grind them into powder, sieve them, and prepare mulberry fruit powder, so that mulberries can supplement the carbon source and trace elements required by probiotics, and are beneficial to the body's anti-oxidation and anti-aging.

[0061] Oyster peptide is a small molecule oligopeptide that completely retains the original vitamins, trace elements, taurine and other nutrients of oysters. Oysters rich in nucleic acid are absorbed faster than single amino acids or proteins after ingestion by the human body and are easier to be absorbed by the body. It has more important biological activity in human metabolism and can effectively increase male serum testosterone levels.

[0062] The extraction method of oyster peptides includes the following steps:

[0063] 1. Cleaning steps: Clean the oysters according to the set cleaning time to form clean oysters.

[0064] 2. Steaming step: put water into the steaming device, put in the oysters by weight while heating, and stir simultaneously, stop heating after the temperature reaches the set insulation range, and keep warm according to the set insulation time, stop stirring after the insulation ends, and then let it stand according to the set standing time to form a soup.

[0065] 3. Soup draining step: extracting the primary soup using an extraction device and pumping it into a primary soup storage device.

[0066] 4. Hydrolysis step: pump the primary soup in the primary soup storage device into the hydrolysis device, heat it to the set sterilization temperature and maintain it for the set sterilization time, then cool it to the set enzymatic hydrolysis temperature, add the configuration liquid to adjust the pH value of the primary soup to neutral, add the animal protease preparation for hydrolysis, and maintain it for the set enzymatic hydrolysis time, after the enzymatic hydrolysis is completed, heat it to the set enzyme inactivation temperature and maintain it for the set enzyme inactivation time to form an enzyme inactivated hydrolyzate.

[0067] 5. Filtration step: the enzyme-killed hydrolyzate is put into diatomaceous earth and / or activated carbon and allowed to stand, and filtered into a clear liquid storage device using a filtering device, and then the clear liquid is circulated and filtered according to the set circulation filtration time using a circulation filtration device.

[0068] 6. Concentration step: Concentrate the clear liquid after the circulating filtration to a set concentration to form a clarified concentrated liquid.

[0069] 7. Spray drying step: spray drying the clarified concentrated liquid according to the set air inlet temperature and the set air outlet temperature to form oyster peptide powder.

[0070] Sea cucumber peptide is an extract of sea cucumber. It can regulate human functions, repair damaged cells, enhance human immunity, and has a series of effects such as anti-fatigue and anti-aging. The preparation method of sea cucumber peptide includes cleaning, extraction, enzymolysis, filtration, concentration and drying in sequence; the cleaning process is to soak the dry sea cucumber in deionized water at room temperature for 8h-10h, and the volume of deionized water is 4-6 times that of the dry sea cucumber; then the soaked dry sea cucumber is cleaned with deionized water. The extraction process is to first crush the sea cucumber and grind it, add deionized water equivalent to 8-9 times the weight of the raw material sea cucumber, then heat it to 110℃~120℃, keep it warm for 25min~50min, filter to remove insoluble residue, and obtain sea cucumber extract. The concentration process includes: evaporating and concentrating the filtered sea cucumber peptide solution to a solid content of 25% to 35% in the sea cucumber peptide solution, the evaporation concentration temperature is 55°C to 65°C, and the evaporation concentration pressure is controlled at -0.4MPa to -0.6MPa. The drying process includes: spray drying the concentrated sea cucumber peptide concentrate, the spray drying inlet temperature is 180°C to 190°C, and the outlet temperature is 85°C to 95°C, and the small molecule sea cucumber peptide powder is obtained after drying.

[0071] Krill peptides are made from Antarctic krill, which can significantly increase the survival time, oxygen consumption and swimming time of mice, increase LDH, liver glycogen content, LA clearance rate and liver and spleen weight; reduce LA and BUA content, and can increase the proliferation rate of mouse spleen lymphocytes, delayed hypersensitivity, antibody-producing cell number, serum hemolysin level, phagocyte carbon clearance ability and NK cell activity, and have the effects of significantly improving anti-fatigue, hypoxia resistance and anti-aging, and improving immunity. Krill peptides are made from fresh Antarctic krill or frozen Antarctic krill, which are crushed and then enzymatically hydrolyzed with trypsin, pepsin and papain.

[0072] In some embodiments of the present invention, the probiotics include one or more of Lactobacillus paracasei JLPF-131, Lactobacillus acidophilus JYLA-191 and Bifidobacterium lactis JYBR-390.

[0073] According to Chinese patent application 202110072512.9, the Lactobacillus paracasei JLPF-131 was deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration on July 8, 2019, with the deposit number: CGMCC NO.18098. Lactobacillus paracasei JLPF-131 can effectively improve high blood pressure, reduce body weight, and prevent obesity. At the same time, it can further reduce blood pressure by reducing the level of total cholesterol and low-density lipoprotein and increasing the level of high-density lipoprotein.

[0074] According to Chinese patent application 202110237827.4, the Lactobacillus acidophilus JYLA-191 in this article is deposited by the General Microbiology Center of the China Microbiological Culture Collection Administration, and its deposit number is: CGMCC No. 21371, and its deposit date is December 14, 2020. The Lactobacillus acidophilus JYLA-191 bacteria of the present invention can combine with cholesterol in the intestine, slow down the absorption of cholesterol, reduce the formation rate of lipoproteins in the blood, and reduce the cholesterol content in the circulatory system. The co-precipitation of Lactobacillus acidophilus JYLA-191 bacteria, free bile salts, and cholesterol accelerates the conversion of bile salts from a bound state to a free state, resulting in the secretion of more bile salts, which speeds up the metabolism of steroid substances in the digestive tract. In addition, co-precipitation with cholesterol can accelerate the conversion of cholesterol into bile acid in the liver, thereby reducing the cholesterol content in the blood.

[0075] According to Chinese patent application 202111479649.2, Bifidobacterium lactis JYBR-390 has the characteristics of strong intestinal colonization ability and ability to inhibit pathogens. It is used to prepare a product for treating constipation. The product has an obvious laxative effect. It was deposited in the General Microbiology Center of the China National Microbiological Culture Collection Administration on July 8, 2019, with the deposit number CGMCC NO.18093.

[0076] In the technical solution and some embodiments of the present invention, one or more of polygonatum sibiricum powder, raspberry powder and wolfberry powder can also be added.

[0077] Polygonatum sibiricum is a traditional Chinese medicine, which is the dried rhizome of Yunnan Polygonatum sibiricum, Polygonatum sibiricum or Polygonatum sibiricum of the Liliaceae family. It is often used for spleen and stomach qi deficiency, fatigue, insufficient stomach yin, dry mouth and poor appetite, dry cough due to lung deficiency, hemoptysis caused by overwork, insufficient essence and blood, soreness of waist and knees, premature graying of hair, internal heat and thirst. Polygonatum sibiricum powder is made by grinding ginger-shaped Polygonatum sibiricum, Polygonatum sibiricum and / or Polygonatum sibiricum into powder.

[0078] According to the records in "Ben Cao" and "Ming Yi Bie Lu" and other classics, raspberry has the effects of nourishing the kidney, consolidating essence, reducing urination, nourishing the liver and improving eyesight, anti-cancer, anti-oxidation, anti-aging, anti-bacterial and anti-inflammatory. It is often used to treat symptoms such as spermatorrhea, enuresis, frequent urination, impotence, premature ejaculation, blurred vision, etc. Preparation of raspberry powder: a. add water to raspberries and boil them, keep them slightly boiling for 1.5 hours, ensure that the raspberries are completely immersed during the boiling process, discharge the first extract, cover the liquid outlet with a filter bag for coarse filtration, and pressurize the raw materials in the pot with a stainless steel sieve plate to discharge the extract; b. add water to the filter residue for a second boiling, keep them slightly boiling for 1 hour, and discharge the second extract; c. combine the two extracts and send them to a storage tank, cool them to room temperature and let them stand for 24 hours, take the supernatant and filter it twice with a microporous filter membrane or cotton cake; d. inject the extract into a vacuum concentration tank, concentrate it to 45% of water content and store it for later use; e. dry the concentrated extract into extract powder by spray drying to obtain raspberry powder.

[0079] Lycium barbarum contains rich Lycium barbarum polysaccharides, which can promote immunity, anti-aging and anti-fatigue. Lycium barbarum fruit is dried and ground into powder to obtain Lycium barbarum powder.

[0080] Comparative Example 1

[0081] The probiotic beverage was prepared in a manner substantially the same as in Example 1, except that 80 portions of prebiotics were weighed to replace the mixed fruit powder and the peptide; the remaining parameters, operations and preparation methods were the same as in Example 1.

[0082] Comparing the core performance data of the product (drink) prepared in comparative example 1 and the products (drinks) prepared in examples 1 to 10, it can be seen that after the mixed fruit powder and polypeptide replace the prebiotics, the number of viable probiotics is basically the same. Therefore, the mixed fruit powder and polypeptide can provide the probiotics with the carbon source, nitrogen source and trace elements required for growth and reproduction, avoiding the influence of prebiotics on the intestine. The specific comparison results are as follows Figure 1 The sensory, flavor and viable bacteria count data of the beverages in each embodiment and comparative example are shown in the table.

[0083] The present invention has been introduced in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

[0084] Through the description of the above embodiments, those skilled in the art can clearly understand that the present invention can be implemented. Of course, the above listed situations are only examples, and the present invention is not limited thereto. Those skilled in the art should understand that other variations or simplifications according to the technical solution of the present invention can be appropriately applied to the present invention and should be included in the scope of the present invention.

Claims

1. A mixed fruit powder, polypeptide, and probiotic composition, characterized in that: Its active ingredients include the following components in parts by weight: 45-60 parts of mixed fruit powder, 30-50 parts of polypeptides and 5-25 parts of probiotics; wherein the mixed fruit powder includes a mixture of two or more of cherry fruit powder, roxburghii fruit powder, cranberry fruit powder, pomegranate fruit powder, sea buckthorn fruit powder and mulberry fruit powder; the polypeptide includes one or more of oyster peptide, sea cucumber peptide or krill peptide; the probiotics include one or more of Lactobacillus paracasei JLPF-131, Lactobacillus acidophilus JYLA-191 or Bifidobacterium lactis JYBR-390.

2. The mixed fruit powder, polypeptide and probiotic composition according to claim 1, characterized in that: The mixed fruit powder comprises the following components in parts by weight: 20-40 parts of seabuckthorn fruit powder and 5-10 parts of mulberry fruit powder; or 20-40 parts of seabuckthorn fruit powder and 5-10 parts of roxburghii fruit powder.

3. The mixed fruit powder, polypeptide and probiotic composition according to claim 1, characterized in that: The mixed fruit powder comprises the following components in parts by weight: 20-40 parts of seabuckthorn fruit powder, 5-10 parts of mulberry fruit powder, 5-10 parts of cherry fruit powder and 5-10 parts of cranberry fruit powder.

4. The mixed fruit powder, polypeptide and probiotic composition according to claim 1, characterized in that: The mixed fruit powder comprises the following components in parts by weight: 20-40 parts of seabuckthorn fruit powder, 5-10 parts of mulberry fruit powder, 5-10 parts of roxburghii fruit powder and 5-10 parts of pomegranate fruit powder.

5. The mixed fruit powder, polypeptide and probiotic composition according to claim 1, characterized in that: The mixed fruit powder comprises the following components in parts by weight: 20-40 parts of sea buckthorn fruit powder, 1-5 parts of mulberry fruit powder, 1-5 parts of cherry fruit powder, 1-5 parts of cranberry fruit powder, 1-5 parts of roxburghii fruit powder and 1-5 parts of pomegranate fruit powder.

6. The mixed fruit powder, polypeptide and probiotic composition according to claim 1, characterized in that: The polypeptide comprises the following components in parts by weight: 10-15 parts of oyster peptide, 10-15 parts of sea cucumber peptide and 10-15 parts of krill peptide.

7. The mixed fruit powder, polypeptide and probiotic composition according to claim 1, characterized in that: The probiotics include the following components in parts by weight: 5 to 10 parts by weight of Lactobacillus paracasei JLPF-131, 5 to 10 parts by weight of Lactobacillus acidophilus JYLA-191 and 5 to 10 parts by weight of Bifidobacterium lactis JYBR-390.

8. The mixed fruit powder, polypeptide and probiotic composition according to claim 1, characterized in that: It also includes one or more of polygonatum powder, raspberry powder and wolfberry powder.

9. The mixed fruit powder, polypeptide and probiotic composition according to claim 8, characterized in that: The invention comprises the following components in parts by weight: 1 to 5 parts of polygonatum sibiricum powder, 1 to 5 parts of raspberry powder and 1 to 5 parts of wolfberry powder.

10. A compound beverage using a mixture of fruit powder, polypeptide and probiotic composition, characterized in that: The raw materials include the following weight percentages: 75% to 85% of water and 15% to 25% of the mixed fruit powder, polypeptide and probiotic composition, and the sum of the weight percentages of the water and the mixed fruit powder, polypeptide and probiotic composition is 100%.

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