Plant growth regulator for increasing tillering buds of blackberry lily as well as preparation method and application of plant growth regulator

Abstract

The invention discloses a plant growth regulator for increasing tillering buds of blackberry lily as well as a preparation method and application of the plant growth regulator, and relates to the technical field of blackberry lily planting, and the plant growth regulator is prepared from the following components: 200 to 700 mg / L of 6-benayl aminopurine, 200 to 500 mg / L of naphthylacetic acid and 300 to 800 mg / L of indolebutyric acid. The growth regulator can effectively improve the tillering capability of the blackberry lily, accelerate the propagation speed and realize large-scale production of the blackberry lily.

Description

Technical Field

[0001] The invention relates to the technical field of Belamcanda chinensis planting, and more particularly to a plant growth regulator for increasing tillering buds of Belamcanda chinensis, and a preparation method and application thereof. Background Art

[0002] Belamcanda chinensis L. is a perennial herb of the genus Belamcanda in the family Iridaceae. It has bright colors, beautiful plant shape, strong adaptability, high ornamental value, and is widely used in gardens. It can be widely used in understory ornamental vegetation, green space planting, grassland embellishment, patterned flower beds, etc. It is a special flower resource with great ecological development and utilization value in the Northeast Forest Region. Under natural growth conditions, the tillering ability of Belamcanda is often limited by many factors, and the tillering reproduction coefficient is small, making it difficult to give full play to its growth potential. This not only affects the luxuriance of Belamcanda plants, limits the large-scale promotion and application of fine varieties, but also restricts its ornamental effect and commercial value to a certain extent.

[0003] Tillering is a special branching characteristic formed during the growth and development of plants. Tillering determines the robustness level of individual development and the number of populations under reasonable cultivation conditions. Plant growth regulators can promote the germination of plant lateral buds. External application of plant growth regulators will enhance the tillering ability of plants, greatly improve the tillering reproduction coefficient, accelerate the reproduction speed, and achieve large-scale production. Plant growth regulators play an important role in the occurrence and growth and development of tillering buds. Existing technologies are all general regulators that can promote plant growth and tillering, which are not very targeted.

[0004] Therefore, using Belamcanda chinensis as a material and targeting the specificity of its tillering ability, developing an efficient and targeted plant growth regulator to significantly improve its tillering ability is a technical problem that needs to be solved urgently by technicians in this field. Summary of the invention

[0005] In view of this, the present invention provides a plant growth regulator for increasing tillering buds of Belamcanda chinensis, and a preparation method and application thereof.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] A first aspect of an embodiment of the present invention provides a plant growth regulator for increasing tillering buds of Belamcanda chinensis, comprising the following components:

[0008] 6-Benzylaminopurine 200-700 mg / L, naphthylacetic acid 200-500 mg / L and indolebutyric acid 300-800 mg / L.

[0009] The second aspect of the embodiment of the present invention provides a method for preparing the above-mentioned plant growth regulator, using water as a solvent, adding 6-benzylaminopurine, naphthylacetic acid and indolebutyric acid thereto to make their final concentrations of 200-700 mg / L, 200-500 mg / L and 300-800 mg / L, respectively, and mixing them evenly to obtain a plant growth regulator.

[0010] The third aspect of the embodiments of the present invention provides the use of the plant growth regulator in promoting tillering of Belamcanda oleifera.

[0011] The fourth aspect of the embodiments of the present invention provides a method for increasing tillering buds of Belamcanda chinensis. When the Belamcanda chinensis seedlings grow to a height of 5 to 8 cm, the plant growth regulator is used for root irrigation at a concentration of 50 to 80 ml per plant, once every 7 to 10 days, and continuously for 3 to 4 times.

[0012] In the preliminary test, it was found that the effect of using a single plant growth regulator on the tillering of Belamcanda chinensis was not obvious. Compared with the single agent treatment, the compound treatment was more effective. From the distribution of endogenous hormone content and their ratios, it is difficult to judge which hormone plays a decisive regulatory role. In fact, the concentration effect of a single hormone is not only affected by the effects of other hormones, but also depends on its concentration at a special action site and the sensitivity of plant cells to it, that is, for the entire growth and development process, various hormones play a synergistic and chain role. The inventor tried a combination of various plant growth regulators. After many ratio tests, it was found that the combined use of 6-benzylaminopurine, naphthylacetic acid and indolebutyric acid can effectively improve the tillering ability of Belamcanda chinensis within a specific ratio range, and synergistically enhance the effect. When the combination of 6-BA, NAA and IBA was used in the concentration ratio of 500mg / L×300mg / L×500mg / L, the tillering proliferation effect was the best, and the tillering coefficient reached 6.1, which was more significant than the effect of single plant growth regulator treatment on the tillering ability of Belamcanda chinensis, achieving the goal of large-scale production. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0014] Figure 1 The attached figure shows the tillering situation of Belamcanda chinensis plants when treated with different combinations of plant growth regulators. DETAILED DESCRIPTION

[0015] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0016] Example 1

[0017] Using water as solvent, 6-benzylaminopurine, naphthylacetic acid and indolebutyric acid were added thereto to make the final concentrations of 600 mg / L, 400 mg / L and 600 mg / L respectively, and the mixture was mixed evenly to obtain a plant growth regulator.

[0018] Example 2

[0019] Using water as solvent, 6-benzylaminopurine, naphthylacetic acid and indolebutyric acid were added thereto to make the final concentrations of 400 mg / L, 300 mg / L and 600 mg / L respectively, and the mixture was mixed evenly to obtain a plant growth regulator.

[0020] Example 3

[0021] Using water as solvent, 6-benzylaminopurine, naphthylacetic acid and indolebutyric acid were added thereto to make the final concentrations of 500 mg / L, 300 mg / L and 500 mg / L respectively, and the mixture was mixed evenly to obtain a plant growth regulator.

[0022] Example 4

[0023] A method for increasing tillering buds of Belamcanda chinensis:

[0024] Step 1. Obtain plants: The best time to cut the rhizomes of Belamcanda chinensis is in spring. Select plants with a diameter of 3 to 4 cm as mother plants. Select the current-year lateral buds with a diameter of 1 to 1.5 cm on the mother plants, separate them from the rhizomes of the mother plants, and then soak them in a 500-fold solution of 25% carbendazim wettable powder for 1 hour for sterilization. The tools used for cutting should be disinfected in advance, and be as careful as possible to avoid damaging the young buds during operation.

[0025] Step 2, planting: Plant the lateral buds of the current year treated in step 1 into a flower pot filled with a substrate; the substrate is a mixture of garden soil, peat and river sand, and the volume ratio of the substrate is 3:1:1, and all the Belamcanda plants are kept at the same temperature, humidity and light;

[0026] Step 3, applying regulators: When the plants planted in step 2 grow to a height of 5 to 8 cm, the plant growth regulator combination formula of Example 1 is used for treatment, and the roots of the Belamcanda oleifera are watered at a rate of 50 to 80 ml / plant. Watering is performed once every 7 days for 3 consecutive times, which can promote the tillering coefficient of the Belamcanda oleifera to be increased.

[0027] The treatment method of the control group was: watering without adding any plant growth regulators, and the amount of watering for the roots was 50-80 ml / plant. Watering was done once every 8 days for 4 consecutive times.

[0028] The experimental results showed that the tillering coefficient of the treated Belamcanda ternata reached 4.4, which was 1.85 times that of the control sample, and the plants were in good growth condition.

[0029] Example 5

[0030] A method for increasing tillering buds of Belamcanda chinensis:

[0031] Step 1, obtain plants: The best time to cut the rhizomes of Belamcanda chinensis is in spring. Select plants with a diameter of 3 to 4 cm as mother plants, select the current-year lateral buds with a diameter of 1 to 1.5 cm on the mother plants, separate them from the rhizomes of the mother plants, and then soak them in a 500-fold solution of 25% carbendazim wettable powder for 0.7 hours for sterilization. The tools used for cutting should be disinfected in advance, and be as careful as possible to avoid damaging the young buds during operation;

[0032] Step 2, planting: Plant the lateral buds of the current year treated in step 1 into a flower pot filled with a substrate; the substrate is a mixture of garden soil, peat and river sand, and the volume ratio of the substrate is 3:1:1, and all the Belamcanda plants are kept at the same temperature, humidity and light;

[0033] Step 3, applying a regulator: When the plants planted in step 2 grow to a height of 5 to 8 cm, the plant growth regulator of Example 3 is used for treatment, and the roots of the Belamcanda oleifera are watered at an amount of 50 to 80 ml / plant. Watering is performed once every 8 days for 4 consecutive times, which can promote the tillering coefficient of the Belamcanda oleifera to be increased.

[0034] The treatment method of the control group was: watering without adding any plant growth regulators, and the amount of watering for the roots was 50-80 ml / plant. Watering was done once every 8 days for 4 consecutive times.

[0035] The experimental results showed that the tillering coefficient of the treated Belamcanda ternata reached 6.1, which was 2.57 times that of the control sample. The plants were in good growth condition and the overall ornamental value was significantly improved.

[0036] Example 6

[0037] A method for increasing tillering buds of Belamcanda chinensis:

[0038] Step 1, obtain plants: The best time to cut the rhizomes of Belamcanda chinensis is in spring. Select plants with a diameter of 3 to 4 cm as mother plants, select the current year's lateral buds with a diameter of 1 to 1.5 cm on the mother plants, separate them from the rhizomes of the mother plants, and then soak them in 500 times the liquid of 25% carbendazim wettable powder for 0.5 hours for sterilization. The tools used for cutting should be disinfected in advance, and be as careful as possible to avoid damaging the young buds during operation;

[0039] Step 2, planting: Plant the lateral buds of the current year treated in step 1 into a flower pot filled with a substrate; the substrate is a mixture of garden soil, peat and river sand, and the volume ratio of the substrate is 3:1:1, and all the Belamcanda plants are kept at the same temperature, humidity and light;

[0040] Step 3: When the plants planted in step 2 grow to a height of 5 to 8 cm, the plant growth regulator of Example 2 is used for treatment, and the roots of the Belamcanda oleifera are watered at a rate of 50 to 80 ml per plant. Watering is performed once every 10 days for 3 consecutive times, which can promote the tillering coefficient of the Belamcanda oleifera to be increased.

[0041] The treatment method of the control group was: watering without adding any plant growth regulators, and the amount of watering for the roots was 50-80 ml / plant. Watering was done once every 8 days for 4 consecutive times.

[0042] The experimental results showed that the tillering coefficient of the treated Belamcanda ternata reached 5.3, which was 2.23 times that of the control sample, and the plants were in good growth condition.

[0043] Control example: In the preliminary test, we found that the use of a single plant growth regulator had no obvious effect on the tillering of Belamcanda chinensis, as shown in Table 1.

[0044] Table 1 Single factor and level design

[0045]

[0046]

[0047] Note: Tillering coefficient = average tillering number - 1; Different lowercase letters next to numbers in the same column indicate significant differences (P<0.05), the same as in the following table.

[0048] Comparison example

[0049] Compared with the single agent treatment, the combined treatment had a more obvious effect. When 6-BA was combined with NAA or 6-BA was combined with IBA, it had a significant effect on the tillering of Belamcanda chinensis. See Table 2 for details.

[0050] Table 2 Two-factor and level design

[0051]

[0052] On this basis, we tried a variety of plant growth regulator combination treatment methods. After many ratio tests, we found that the combined application of 6-benzylaminopurine, naphthylacetic acid and indolebutyric acid can more effectively improve the tillering ability of Belamcanda chinensis within a specific ratio range, and achieve synergistic effects. Figure 1 and Table 3.

[0053] Table 3 Three factors and level design

[0054]

[0055]

[0056] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0057] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A plant growth regulator for increasing tillering buds of Belamcanda chinensis, characterized in that: It is composed of the following ingredients: 6-Benzylaminopurine 200-700 mg / L, naphthylacetic acid 200-500 mg / L and indolebutyric acid 300-800 mg / L.

2. The method for preparing the plant growth regulator according to claim 1, characterized in that: Using water as solvent, 6-benzylaminopurine, naphthylacetic acid and indolebutyric acid are added thereto to make their final concentrations respectively 200-700 mg / L, 200-500 mg / L and 300-800 mg / L, and the mixture is mixed evenly to obtain a plant growth regulator.

3. Use of the plant growth regulator according to claim 1 in promoting tillering of Belamcanda chinensis.

4. A method for increasing tillering buds of Belamcanda chinensis, characterized in that: When the seedlings of Belamcanda chinensis grow to a height of 5 to 8 cm, the plant growth regulator according to claim 1 is used for root irrigation at a rate of 50 to 80 ml per plant, once every 7 to 10 days, and continuously for 3 to 4 times.

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