Metal-modified antibacterial mask as well as preparation method and application thereof

Abstract

The invention provides a metal-modified antibacterial mask as well as a preparation method and application thereof. The metal-modified antibacterial mask sequentially comprises a non-woven fabric, an antibacterial layer and a non-woven fabric from inside to outside, wherein the antibacterial layer is a fiber material loaded with a metal coating, and the metal-loaded coating is realized by soaking the fiber material in a metal ion precursor solution and adding a reducing agent to perform an in-situ reduction reaction; the fiber material loaded with the metal coating and the bacteria are co-cultured, growth and reproduction of the bacteria can be effectively inhibited, and metal ions form a uniform and stable antibacterial layer on the antibacterial layer, so that a lasting and long-acting sterilization effect is achieved, and efficient removal of the bacteria is realized.

Description

Technical Field

[0001] The invention relates to the technical field of nanomedicine, and in particular to a preparation method and application of a metal-modified antibacterial mask. Background Art

[0002] Masks are a kind of conventional protective equipment used to filter the air entering the mouth and nose, and play an important role in preventing and blocking the spread of germs. For bacterial microorganisms, wearing masks can also largely prevent them from spreading through air, saliva, droplets, etc., thereby reducing the harm caused by pathogens to human health. However, ordinary medical masks mainly intercept microorganisms through physical barriers but cannot kill bacteria. Bacteria will continue to multiply on the non-woven fabric outside the mask, and pathogens may even spread from person to person again, which is a huge hidden danger to human health and environmental hygiene.

[0003] The rapid development of metal coating technology has provided new ideas and methods for the further development and application of medical antibacterial masks. Metal coating can give masks an efficient sterilization function, and can kill common bacteria (Mycobacterium tuberculosis, Pseudomonas aeruginosa, Acinetobacter baumannii) without other auxiliary conditions (light, heat), making it more and more widely used in medical and health care, food packaging and public facilities.

[0004] Ordinary medical masks intercept microorganisms through physical barriers but cannot kill bacteria. The risks of microbial reproduction, secondary transmission, and hidden dangers to human health and the environment are problems that have not been solved.

[0005] Therefore, the related technology of the preparation method of the novel antibacterial mask in the prior art still needs to be further improved. Summary of the invention

[0006] In view of this, the present invention proposes a metal-modified antibacterial mask and a preparation method and application thereof, which solve the technical problems in the prior art that conventional masks cannot kill bacteria, cause microbial reproduction or secondary transmission, and pose hidden dangers to human health and the environment.

[0007] The technical solution of the present invention is achieved in this way:

[0008] In one aspect, the present invention provides a metal-modified antibacterial mask, which comprises, from inside to outside, a non-woven fabric, an antibacterial layer, and a non-woven fabric;

[0009] Wherein, the antibacterial layer is a fiber material loaded with a metal coating, and the loaded metal coating is achieved by soaking the fiber material in a metal ion precursor solution and adding a reducing agent to carry out an in-situ reduction reaction.

[0010] On the basis of this technical solution, further preferably, the fiber material includes gauze or non-woven fabric.

[0011] In a second aspect, based on this technical solution, the present invention also provides a method for preparing the metal-modified antibacterial mask described in the first aspect, comprising the following steps:

[0012] The fiber material is immersed in a metal ion precursor solution, a reducing agent is added, and an in-situ reduction reaction is performed at 25-29° C., so that a metal coating is formed on the surface of the fiber material, thereby obtaining a fiber material loaded with a metal coating;

[0013] Cleaning the fiber material loaded with the metal coating, and drying it to obtain the antibacterial layer;

[0014] The non-woven fabric, the antibacterial layer and the non-woven fabric are assembled in sequence from the inside to the outside to obtain the metal-modified antibacterial mask.

[0015] On the basis of this technical solution, further preferably, the metal ion precursor solution includes one of a silver ammonia solution, a copper ammonia solution or a zinc ammonia solution.

[0016] On the basis of this technical solution, further preferably, the concentration of the metal ion precursor solution is 5 to 15 wt %.

[0017] On the basis of this technical solution, it is further preferred that the soaking time is 36 to 48 hours, and the drying conditions are 45-60° C. and drying for 5-7 hours.

[0018] On the basis of this technical solution, further preferably, the reducing agent includes glucose or ascorbic acid, and the reaction time of adding the reducing agent is 30 min-180 min.

[0019] On the basis of this technical solution, it is further preferred that the molar ratio of the reducing agent to the metal ion precursor solution is 1:10 to 1:20.

[0020] In a third aspect, the present invention further provides the use of the antibacterial mask obtained by the metal-modified antibacterial mask described in the first aspect or the preparation method of the metal-modified antibacterial mask described in the second aspect in food packaging and medical surgery.

[0021] The metal-modified antibacterial mask, preparation method and application provided by the present invention have the following beneficial effects compared with the prior art:

[0022] The antibacterial layer is the key part, which is composed of fiber materials loaded with metal coatings. The fiber materials loaded with metal coatings use metal ion precursors (such as silver ammonia solution, copper ammonia solution, etc.) and fiber materials (gauze, non-woven fabrics, etc.) as raw materials. By using at least one reducing agent such as glucose and ascorbic acid, the metal ions are reduced to metal elements on the surface of the fiber material, and then deposited on the surface of the fiber material to form a metal coating.

[0023] The fiber material loaded with metal coating is co-cultured with bacteria to effectively inhibit the growth and reproduction of bacteria. The metal ions form a uniform and stable antibacterial layer in the antibacterial layer, thereby achieving a long-lasting bactericidal effect and realizing efficient removal of bacteria. Highly efficient antibacterial function can be achieved without any external conditions (light, heat).

[0024] The new antibacterial mask can effectively avoid the secondary pollution of the environment caused by discarded masks; the required preparation materials are green and pollution-free, meeting the requirements of sustainable development; the preparation method is simple and easy to operate and promote. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 Schematic diagram of ordinary woven cotton cloth and antibacterial layer in Example 1 of the present invention;

[0027] Figure 2 This is a SEM and EDS schematic diagram of the antibacterial layer of Example 2 of the present invention;

[0028] Figure 3 This is the XPS spectrum of the antibacterial layer of Example 2 of the present invention;

[0029] Figure 4 This is a schematic diagram of ROS induction of the antibacterial layer of Application Example 1 of the present invention;

[0030] Figure 5 The antibacterial performance results of the antibacterial layer of Application Example 1 of the present invention against Mycobacterium tuberculosis (M. tuberculosis), Pseudomonas aeruginosa (P. aeruginosa) and Acinetobacter baumannii (A. baumannii) are shown. DETAILED DESCRIPTION

[0031] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] The invention provides a metal-modified antibacterial mask, a preparation method and an application thereof, which can realize a high-efficiency antibacterial function without any external conditions (light, heat); the antibacterial layer of the mask is a fiber material loaded with a metal coating, which is covered with a layer of uniform and stable metal coating, thereby achieving a long-lasting and long-lasting bactericidal effect; the preparation process of the antibacterial layer comprises taking 5 pieces of textile cotton cloth with a specification of 2cm*3cm, placing them in a 200ml copper ammonia solution in a soaking form, standing for 48h, adding 15g of ascorbic acid and standing for 60min, washing with deionized water, and drying at room temperature to obtain the antibacterial layer of the copper metal coating.

[0033] Example 1

[0034] A metal-modified antibacterial mask, comprising, from inside to outside, a non-woven fabric, an antibacterial layer, and a non-woven fabric;

[0035] Wherein, the antibacterial layer is a fiber material loaded with a metal coating, and the loaded metal coating is achieved by soaking the fiber material in a metal ion precursor solution and adding a reducing agent to carry out an in-situ reduction reaction.

[0036] In a preferred embodiment, specifically, the fiber material comprises gauze.

[0037] In a preferred embodiment, specifically, the method for preparing the metal-modified antibacterial mask comprises the following steps:

[0038] Weigh 7.84 g of copper nitrate and add it to 100 ml of deionized water to dissolve, then add 7.35 ml of concentrated ammonia water dropwise until a blue precipitate is produced, and continue to add 14.65 ml of concentrated ammonia water dropwise until it is completely dissolved. Finally, adjust the volume to prepare 200 ml of 5 wt% copper ammonia solution.

[0039] Cut 8 pieces of textile cotton cloth with a specification of 2cm*3cm, soak them in 200ml of copper ammonia solution, let them stand for 48h, add 15g of ascorbic acid and let them stand for 60min, wash them with deionized water, and dry them at room temperature to obtain the antibacterial layer of copper metal coating.

[0040] After assembling the outer non-woven fabric, the antibacterial layer and the inner non-woven fabric, a new type of antibacterial mask modified with metal is obtained. A control group (ordinary textile cotton cloth) and a treatment group (antibacterial layer) are set up. ，Material characterization and analysis were carried out separately: scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS).

[0041] In a preferred embodiment, specifically, the concentration of the metal ion precursor solution is 5 wt %.

[0042] In a preferred embodiment, specifically, the reducing agent includes glucose or ascorbic acid.

[0043] In a preferred embodiment, specifically, the molar ratio of the reducing agent to the metal ion precursor solution is 1:25.

[0044] result: Figure 1 Schematic diagram of ordinary woven cotton cloth and antibacterial layer in Example 1 of the present invention, Figure 1 As shown, it can be seen that the obtained antibacterial layer is uniform light blue.

[0045] Example 2

[0046] A metal-modified antibacterial mask, comprising, from inside to outside, a non-woven fabric, an antibacterial layer, and a non-woven fabric;

[0047] Wherein, the antibacterial layer is a fiber material loaded with a metal coating, and the loaded metal coating is achieved by soaking the fiber material in a metal ion precursor solution and adding a reducing agent to carry out an in-situ reduction reaction.

[0048] In a preferred embodiment, specifically, the fiber material comprises non-woven fabric.

[0049] In a preferred embodiment, specifically, the method for preparing the metal-modified antibacterial mask comprises the following steps:

[0050] Weigh 19.77g of silver nitrate and add it to 100ml of deionized water to dissolve it. Add 8.68ml of concentrated ammonia water dropwise until a brown-yellow precipitate is produced. Continue to add 17.37ml of concentrated ammonia water dropwise until it is completely dissolved. Finally, make up to volume to prepare 200ml of 10wt% silver ammonia solution.

[0051] Cut 5 pieces of textile cotton cloth with a specification of 2cm*3cm, soak them in 200ml of silver ammonia solution, let them stand for 48h, add 15g of glucose and let them stand for 60min, wash them with deionized water, and dry them at room temperature to obtain the antibacterial layer of silver metal coating.

[0052] After assembling the outer non-woven fabric, the antibacterial layer and the inner non-woven fabric, a new type of metal-modified antibacterial mask is obtained.

[0053] In a preferred embodiment, specifically, the concentration of the metal ion precursor solution is 15 wt %.

[0054] In a preferred embodiment, specifically, the reducing agent includes glucose.

[0055] In a preferred embodiment, specifically, the molar ratio of the reducing agent to the metal ion precursor solution is 1:20.

[0056] result: Figure 2 , 3 They are respectively the SEM, EDS schematic diagram and XPS spectrum of the antibacterial layer of Example 2 of the present invention, as shown in Figure 2 , 3 As shown, the copper ions are evenly distributed on the fiber material.

[0057] Application Example 1

[0058] The bactericidal effect test of the antibacterial mask obtained in Example 1 specifically includes the following steps:

[0059] A control group (with ordinary textile cotton cloth) and a treatment group (with antibacterial layer) were set up. Two pieces of ordinary textile cotton cloth or antibacterial layer (2cm*3cm) were added to 1mM H2O2 solution, left to stand at room temperature, and samples were taken at 0min, 5min, 10min, 15min, 20min, and 30min, respectively. Then, ROS probe DCFH-DA was added and incubated for 30min. The fluorescence intensity of the solution at 488nm, i.e., the amount of ROS produced, was measured using an ELISA instrument.

[0060] Results: Figure 4 As shown, the results indicate that the Fenton-like catalyst activity of copper ions in the antibacterial layer is time-dependent. In the presence of H2O2, copper ions act as Fenton-like catalysts to catalyze H2O2 molecules to produce hydroxyl radicals (OH·), thereby killing bacteria.

[0061] A control group (with ordinary textile cotton cloth) and a treatment group (with antibacterial layer) were set up. 2 mL of Mycobacterium tuberculosis, Pseudomonas aeruginosa and Acinetobacter baumannii (concentration of OD600 = 0.1) were co-incubated with ordinary textile cotton cloth and the antibacterial layer in a 12-well plate, respectively, and cultured in an shaking incubator at 37°C and 150 rpm for 3 h. The antibacterial properties of the material were then verified by the dilution spot plate method and the plate colony counting method.

[0062] Results: The antibacterial layer prepared in Example 1 has the ability to kill Mycobacterium tuberculosis (M.tuberculosis), Pseudomonas aeruginosa (P.aeruginosa) and Acinetobacter baumannii (A.baumannii). Figure 5 As shown in (a), Figure 5 (b) in the figure shows the antibacterial layer covering the metal coating, which has a killing performance of more than 99% for three types of bacteria, indicating that the meltblown layer has a highly efficient bactericidal function.

[0063] In summary, the present invention provides a metal-modified antibacterial mask, a preparation method and an application thereof. An antibacterial layer composed of a fiber material loaded with a metal coating is provided, a metal ion precursor (such as a silver ammonia solution, a copper ammonia solution, etc.) and a fiber material (gauze, non-woven fabric, etc.) are used as raw materials, and at least one reducing agent such as glucose and ascorbic acid is used to reduce the metal ions on the surface of the fiber material to a metal element, which is deposited on the surface of the fiber material to form a metal coating. The fiber material loaded with the metal coating is co-cultured with bacteria, which can effectively inhibit the growth and reproduction of bacteria and achieve efficient removal of bacteria. The metal coating can give the mask an efficient sterilization function, and can kill common bacteria (Mycobacterium tuberculosis, Pseudomonas aeruginosa, Acinetobacter baumannii) without other auxiliary conditions (light, heat), so that it is more and more widely used in medical and health care, food packaging and public facilities.

[0064] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A metal-modified antibacterial mask, characterized in that: From inside to outside, it includes: non-woven fabric, antibacterial layer, non-woven fabric; Wherein, the antibacterial layer is a fiber material loaded with a metal coating, and the loaded metal coating is achieved by soaking the fiber material in a metal ion precursor solution and adding a reducing agent to carry out an in-situ reduction reaction.

2. The metal-modified antibacterial mask according to claim 1, characterized in that: The fiber material includes gauze or non-woven fabric.

3. The method for preparing a metal-modified antibacterial mask according to any one of claims 1 to 2, characterized in that: The following steps are involved: The fiber material is immersed in a metal ion precursor solution, a reducing agent is added, and an in-situ reduction reaction is performed at 25-29° C., so that a metal coating is formed on the surface of the fiber material, thereby obtaining a fiber material loaded with a metal coating; Cleaning the fiber material loaded with the metal coating, and drying it to obtain the antibacterial layer; The non-woven fabric, the antibacterial layer and the non-woven fabric are assembled in sequence from the inside to the outside to obtain the metal-modified antibacterial mask.

4. The method for preparing the metal-modified antibacterial mask according to claim 3, wherein: The metal ion precursor solution includes one of a silver ammonia solution, a copper ammonia solution or a zinc ammonia solution.

5. The method for preparing the metal-modified antibacterial mask as claimed in claim 3, characterized in that: The concentration of the metal ion precursor solution is 5-15 wt %.

6. The method for preparing the metal-modified antibacterial mask according to claim 3, characterized in that: The drying conditions are 45-60° C. and drying for 5-7 hours.

7. The method for preparing the metal-modified antibacterial mask according to claim 3, characterized in that: The soaking time is 36 to 48 hours.

8. The method for preparing the metal-modified antibacterial mask as claimed in claim 3, characterized in that: The reducing agent includes glucose or ascorbic acid, and the reaction time of adding the reducing agent is 30 min-180 min.

9. The method for preparing the metal-modified antibacterial mask according to claim 3, wherein: The molar ratio of the reducing agent to the metal ion precursor solution is 1:10 to 1:

20.

10. Application of the antibacterial mask obtained by the preparation method of the metal-modified antibacterial mask according to any one of claims 1 to 2 or the metal-modified antibacterial mask according to any one of claims 3 to 9 in food packaging and medical surgery.

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