Herbaceous plant hyaluronic acid pet ear washing liquid and preparation method thereof

Abstract

The invention discloses a herbaceous plant hyaluronic acid pet ear washing liquid and a preparation method thereof, and belongs to the technical field of pet cleaning care agents, the preparation method comprises the following steps: preparing a stabilizer, preparing a dispersing agent, preparing a first extracting solution, preparing a second extracting solution, and mixing; the step of mixing comprises the following steps: mixing the first extracting solution, the second extracting solution, sodium hyaluronate, hydrolyzed hyaluronic acid and a dispersing agent, and performing high-pressure homogenization to obtain the herbaceous plant hyaluronic acid pet ear washing liquid. The herbaceous plant hyaluronic acid pet ear washing liquid prepared by the invention has the effects of cleaning, sterilizing, moisturizing and repairing, and is strong in cleaning power, long in shelf life and good in stability.

Description

Technical Field

[0001] The invention relates to the technical field of cleaning and care agents for pets, and in particular to a herbal hyaluronic acid pet ear cleaning solution and a preparation method thereof. Background Art

[0002] Pets are not only the most loyal and reliable companions of human beings, but also the spiritual pillars of people's daily lives. Pets can provide people with emotional comfort in a unique way, help people reduce the burden of life, and maintain people's physical and mental health. However, if scientific maintenance methods are ignored, the companionship of pets may also have an adverse effect on people's health. Therefore, pet owners must do a good job of immunization, testing, cleaning, disinfection, etc. in accordance with regulations to avoid adverse effects of pet hygiene problems on people's health.

[0003] In pet breeding, pet cleaning and care are of vital importance, especially for their unique physiological structure. For example, the "L-shaped" ear canal of pets such as dogs and cats is prone to accumulation of earwax, ear wax, and necrotic keratinocytes. If it is not cleaned regularly, it will cause bacteria to breed, leading to ear diseases such as otitis externa and otitis interna. These diseases usually manifest as unbearable ear itching, redness and swelling of the auricle and ear canal, abnormal secretions with odor, and in severe cases, it may cause suppuration of the ear and emit a foul odor, and even cause blockage of the ear canal, tissue hyperplasia and hearing loss.

[0004] Given the close relationship between pets and their owners, improper cleaning and care of the "L-shaped" ear canals of pets such as dogs and cats may also pose a risk to the health of their owners. For example, pathogenic bacteria such as Malassezia in the ear canal may be transmitted to humans, causing skin diseases such as pityriasis versicolor, seborrheic dermatitis and folliculitis; mites in the ear canal may induce allergic reactions in humans.

[0005] As a commonly used cleaning and care agent for pets, pet ear wash can clean the ear canal of pets, prevent and treat ear diseases, and ensure the health and comfort of pets. With the continuous expansion of the pet consumption market, the market demand for pet ear wash is also growing. Therefore, the development of efficient and safe pet ear wash has become one of the urgent problems to be solved in the pet cleaning and care agent market.

[0006] Existing pet ear washes contain a large amount of chemical additives. Although they have strong cleaning power, they will irritate the skin of the pet's ear canal during use. Long-term use will lead to a decrease in the antibacterial ability of the pet's ear canal and easily cause inflammation of the skin in the pet's ear canal. In the experiment, it was found that the pet ear wash solution prepared by mixing herbal plant extracts with sodium hyaluronate can utilize the cleaning and bactericidal effects of the herbal plant extracts and the moisturizing and repairing effects of sodium hyaluronate, thereby obtaining a pet ear wash solution with cleaning, sterilization, moisturizing and repairing effects. However, the obtained pet ear wash solution has the following deficiencies: first, the cleaning power of the herbal plant extracts is insufficient, resulting in incomplete cleaning of the earwax, ear wax and necrotic keratinocytes in the pet's ear canal; second, the herbal plant extracts contain a large amount of active ingredients, which are easily ineffective, resulting in a short shelf life of the pet ear wash solution prepared by mixing the herbal plant extracts and sodium hyaluronate; third, the solubility of the herbal plant extracts and sodium hyaluronate is greatly affected by temperature, with a small solubility at low temperatures and a large solubility at high temperatures, resulting in poor stability of the pet ear wash solution prepared by mixing the herbal plant extracts and sodium hyaluronate, specifically, precipitation and stratification are prone to occur when high and low temperatures alternate. Summary of the invention

[0007] In view of the deficiencies in the prior art, the present invention provides a herbal hyaluronic acid pet ear wash liquid and a preparation method thereof. The prepared herbal hyaluronic acid pet ear wash liquid has cleaning, sterilization, moisturizing and repairing effects, and has strong cleaning power, long shelf life and good stability.

[0008] In order to solve the above technical problems, the technical solution adopted by the present invention is as follows: A method for preparing a herbal hyaluronic acid pet ear cleaning solution comprises the following steps: preparing a stabilizer, preparing a dispersant, preparing a first extract, preparing a second extract, and mixing; The stabilizer is prepared by mixing bamboo charcoal powder and distilled water, stirring at a stirring speed of 300-500 rpm for 50-60 min at room temperature, adding sodium dodecylbenzene sulfonate, stirring at a stirring speed of 300-500 rpm for 30-40 min at room temperature, adding cationic hyaluronic acid, stirring at a stirring speed of 300-500 rpm for 30-40 min at room temperature, adding sodium dodecylbenzene sulfonate, stirring at a stirring speed of 300-500 rpm for 50-60 min at room temperature, filtering, washing the filter residue with distilled water, and drying to obtain a stabilizer; In the preparation of the stabilizer, the mass ratio of bamboo charcoal powder, distilled water, sodium dodecylbenzene sulfonate, cationic hyaluronic acid, and sodium dodecylbenzene sulfonate is 200-210 g: 3700-4000 mL: 8.7-9 g: 5-5.2 g: 8.7-9 g; The particle size of the bamboo charcoal powder is 5 μm; The cationic hyaluronic acid has a number average molecular weight of 20 wDa and a degree of substitution of 0.15; The dispersant is prepared by mixing soybean protein and distilled water, stirring at a stirring speed of 300-500 rpm for 2-3 hours at room temperature, standing at 4-6°C for 20-25 hours, adding a sodium hydroxide aqueous solution to adjust the pH to 12-12.5, stirring at a stirring speed of 300-500 rpm for 50-60 minutes at room temperature, adding a hydrochloric acid aqueous solution to adjust the pH to 7-7.5, stirring at a stirring speed of 300-500 rpm for 50-60 minutes at room temperature, and then stirring at a stirring speed of 300-500 rpm for 30-40 minutes at 58-62°C, and stirring at a stirring speed of 300-500 rpm at 68-72°C for 30- 40min, stirring at 78-82°C at a stirring speed of 300-500rpm for 30-40min, stirring at 88-92°C at a stirring speed of 300-500rpm for 30-40min, transferring to an ice water bath and stirring at a stirring speed of 300-500rpm, adding cationic hyaluronic acid aqueous solution and polyethylene glycol 2000 aqueous solution, stirring at a stirring speed of 300-500rpm in an ice water bath for 50-60min, adding sodium dodecylbenzene sulfonate aqueous solution, stirring at a stirring speed of 300-500rpm in an ice water bath for 50-60min, centrifuging, taking a precipitate, washing the precipitate with distilled water, and drying to obtain a dispersant; In the preparation of the dispersant, the mass volume ratio of soybean protein, distilled water, cationic hyaluronic acid aqueous solution, polyethylene glycol 2000 aqueous solution, and sodium dodecylbenzene sulfonate aqueous solution is 50-52g:6000-6500mL:140-160mL:70-75mL:70-80mL; The mass concentration of the sodium hydroxide aqueous solution is 5%; The mass concentration of the hydrochloric acid aqueous solution is 5%; The mass concentration of the cationic hyaluronic acid aqueous solution is 4%; The mass concentration of the polyethylene glycol 2000 aqueous solution is 2%; The mass concentration of the sodium dodecylbenzenesulfonate aqueous solution is 5%; The first extract is prepared by washing and drying honeysuckle, coptis root, matricaria, sophora flavescens, witch hazel, dandelion leaf, and mint respectively, and grinding them to a particle size of 0.3 mm to obtain honeysuckle pollen, coptis root powder, matricaria powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder, and mint powder; then, honeysuckle pollen, coptis root powder, matricaria powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder, mint powder, and distilled water are mixed, and the mixture is allowed to stand at room temperature for 30-40 minutes, stirred and refluxed at 90-95° C. at a stirring speed of 300-400 rpm for 6-7 hours, cooled naturally to room temperature, filtered, and the filtrate is taken, the filtrate is centrifuged, the supernatant is taken, the supernatant is concentrated under reduced pressure to one seventh of the original volume, a stabilizer is added, and the mixture is stirred at room temperature at a stirring speed of 300-400 rpm for 50-60 minutes to obtain the first extract; In the preparation of the first extract, the honeysuckle powder, coptis powder, chamomile powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder, mint powder, and distilled water are used in a ratio of 80-90g:70-80g:100-115g:80-90g:80-90g:140-150g:70-80g:9000-9500mL; The dosage ratio of honeysuckle powder to stabilizer is 80-90g:40-42g; The second extract is prepared by washing aloe leaves and cypress, taking aloe leaf pulp, drying the aloe leaf pulp and cypress, respectively grinding them to a particle size of 0.3 mm to obtain aloe leaf pulp powder and cypress powder, mixing the aloe leaf pulp powder, cypress powder and distilled water, standing at room temperature for 30-40 minutes, stirring and reflux at 90-95° C. at a stirring speed of 300-400 rpm for 4-4.5 hours, cooling naturally to room temperature, filtering, taking the filtrate, and using the filtrate as the second extract; In the preparation of the second extract, the ratio of aloe leaf powder, cypress powder and distilled water is 100-110 g: 300-340 g: 700-760 mL; The mixing comprises mixing the first extract, the second extract, sodium hyaluronate, hydrolyzed hyaluronic acid, and the dispersant, and then high-pressure homogenizing at a pressure of 30-35 MPa for 25-30 minutes at room temperature to obtain a herbal hyaluronic acid pet ear wash solution; In the mixing, the dosage ratio of the first extract, the second extract, sodium hyaluronate, hydrolyzed hyaluronic acid, and the dispersant is 1200-1250 mL: 700-750 mL: 40-43 g: 17-19 g: 80-85 g; The molecular weight of the sodium hyaluronate is 50wDa; The molecular weight of the hydrolyzed hyaluronic acid is 10 kDa.

[0009] A herbal hyaluronic acid pet ear cleaning solution prepared by the above-mentioned preparation method.

[0010] Compared with the prior art, the present invention has the following beneficial effects: (1) The preparation method of the herbal hyaluronic acid pet ear wash liquid of the present invention comprises adding a stabilizer and a dispersant during the preparation of the herbal hyaluronic acid pet ear wash liquid, wherein the preparation method of the stabilizer comprises adsorbing a layer of sodium dodecylbenzene sulfonate on the surface of bamboo charcoal powder, then adding cationic hyaluronic acid, adsorbing the cationic hyaluronic acid on the surface of bamboo charcoal powder through the electrostatic adsorption between the anionic charge of the sodium dodecylbenzene sulfonate and the cationic charge of the cationic hyaluronic acid, then adding sodium dodecylbenzene sulfonate, and continuing to adsorb the sodium dodecylbenzene sulfonate on the surface of the bamboo charcoal powder through the electrostatic adsorption, and finally forming a layer of sodium dodecylbenzene sulfonate on the surface of the bamboo charcoal powder. A coating layer formed by sodium dodecylbenzene sulfonate and cationic hyaluronic acid, i.e., a stabilizer. After the stabilizer is added to the first extract, the bamboo charcoal in the stabilizer will adsorb the active ingredients of the first extract. The coating layer of the outer layer of the stabilizer can improve the stability of the bamboo charcoal after adsorption. In addition, the cationic hyaluronic acid in the coating layer cooperates with sodium dodecylbenzene sulfonate to ensure the cleaning and dispersing abilities of sodium dodecylbenzene sulfonate while avoiding the irritation of sodium dodecylbenzene sulfonate to the skin. The preparation method of the dispersant is to subject soybean protein to pH shift and heat treatment, thereby causing the structure of the soybean protein to change. The solubility of protein is reduced, and cationic hyaluronic acid and polyethylene glycol 2000 are added. Soy protein, cationic hyaluronic acid and polyethylene glycol 2000 are combined by intermolecular forces to form microparticles. Cationic hyaluronic acid and polyethylene glycol 2000 can improve the solubility of soy protein. Then sodium dodecylbenzene sulfonate is added. Sodium dodecylbenzene sulfonate is combined with the cationic hyaluronic acid in the microparticles through electrostatic adsorption to the outermost layer of the microparticles to obtain a dispersant. After the dispersant is mixed with the first extract, the second extract, sodium hyaluronate and hydrolyzed hyaluronic acid, the soy protein and sodium dodecylbenzene sulfonate in the dispersant are both Emulsifier can play a role of synergistic dispersion. During dispersion, soy protein, cationic hyaluronic acid and polyethylene glycol 2000 can be combined with the outermost layer of the substance to be dispersed. Sodium dodecylbenzene sulfonate further coats the substance to be dispersed through electrostatic adsorption, thereby encapsulating the substance to be dispersed in vesicles. The stability of the substance to be dispersed is improved through the vesicle effect. In addition, cationic hyaluronic acid is a polysaccharide, and it cooperates with polyethylene glycol 2000 to avoid the decomposition of the substance to be dispersed through the intermolecular force between the substance to be dispersed and the soy protein combined with cationic hyaluronic acid can also reduce the irritation of sodium dodecylbenzene sulfonate and promote the recovery of otitis. (2) The preparation method of the herbal hyaluronic acid pet ear wash liquid of the present invention has cleaning, sterilization, moisturizing and repairing effects, and has strong cleaning power. After the herbal hyaluronic acid pet ear wash liquid prepared by the present invention is used continuously for treating otitis in dogs and cats for 4 weeks, the symptoms of otitis completely disappear or are significantly improved, and there is no foreign matter or basically no foreign matter inside the ear. According to the QB / T2738-2012 test standard, the sterilization rate for Escherichia coli is 98.2-99.4%, the sterilization rate for Candida albicans is 99.1-99.2%, the sterilization rate for Staphylococcus aureus is 98.1-98.5%, and the sterilization rate for Pseudomonas aeruginosa is 97.5-98.6%; (3) The preparation method of the herbal hyaluronic acid pet ear wash liquid of the present invention has a long shelf life. After the herbal hyaluronic acid pet ear wash liquid prepared by the present invention is sealed and stored at room temperature for 6 months, according to the test standard QB / T 2738-2012, the bactericidal rate against Escherichia coli is 98.1-99.2%, the bactericidal rate against Candida albicans is 98.7-99.1%, the bactericidal rate against Staphylococcus aureus is 97.9-98.2%, and the bactericidal rate against Pseudomonas aeruginosa is 97.1-98.0%; (4) The preparation method of the herbal hyaluronic acid pet ear wash liquid of the present invention has good stability. The herbal hyaluronic acid pet ear wash liquid prepared by the present invention is placed in an environment of -4°C for 24 hours, in an environment of 25°C for 12 hours, and then in an environment of -4°C for 24 hours. After being placed in an environment of 25°C for 12 hours, there is no precipitation and stratification, and the pet ear wash liquid is easy to flow. DETAILED DESCRIPTION

[0011] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, the specific implementation methods of the present invention are now described.

[0012] Example 1 A method for preparing a herbal hyaluronic acid pet ear wash liquid, specifically comprising: 1. Preparation of stabilizer: 200g bamboo charcoal powder and 3700mL distilled water were mixed, stirred at room temperature at a stirring speed of 300rpm for 50min, 8.7g sodium dodecylbenzene sulfonate was added, stirred at room temperature at a stirring speed of 300rpm for 30min, 5g cationic hyaluronic acid was added, stirred at room temperature at a stirring speed of 300rpm for 30min, 8.7g sodium dodecylbenzene sulfonate was added, stirred at room temperature at a stirring speed of 300rpm for 50min, filtered, rinsed the filter residue with distilled water, and dried to obtain a stabilizer; The particle size of the bamboo charcoal powder is 5 μm; The cationic hyaluronic acid has a number average molecular weight of 20 wDa and a degree of substitution of 0.15; 2. Preparation of dispersant: 50 g of soy protein and 6000 mL of distilled water were mixed, stirred at room temperature at a stirring speed of 300 rpm for 2 h, allowed to stand at 4 ° C for 20 h, 5% sodium hydroxide aqueous solution was added to adjust the pH to 12, stirred at room temperature at a stirring speed of 300 rpm for 50 min, 5% hydrochloric acid aqueous solution was added to adjust the pH to 7, stirred at room temperature at a stirring speed of 300 rpm for 50 min, then stirred at 58 ° C at a stirring speed of 300 rpm for 30 min, stirred at 68 ° C at a stirring speed of 300 rpm for 30 min, and stirred at 78 ° C at a stirring speed of 300 rpm for 30 min. 0 rpm for 30 min, at 88 ° C for 30 min at a stirring speed of 300 rpm, transferred to an ice water bath and stirred at a stirring speed of 300 rpm, added 140 mL of a 4% cationic hyaluronic acid aqueous solution and 70 mL of a 2% polyethylene glycol 2000 aqueous solution, stirred at a stirring speed of 300 rpm for 50 min in an ice water bath, added 70 mL of a 5% sodium dodecylbenzene sulfonate aqueous solution, stirred at a stirring speed of 300 rpm for 50 min in an ice water bath, centrifuged, took a precipitate, rinsed the precipitate with distilled water, and dried to obtain a dispersant; 3. Prepare the first extract: wash and dry honeysuckle, coptis chinensis, matricaria, sophora flavescens, witch hazel, dandelion leaf and mint respectively, grind them to a particle size of 0.3 mm to obtain honeysuckle powder, coptis chinensis powder, matricaria powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder and mint powder, then add honeysuckle powder, coptis chinensis powder, matricaria powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder, mint powder and distilled water in an amount ratio of 80g:70g:100g:80g:80g: 140g:70g:9000mL was mixed, and then allowed to stand at room temperature for 30min, stirred and refluxed at 90°C at a stirring speed of 300rpm for 6h, naturally cooled to room temperature, filtered, and the filtrate was taken, the filtrate was centrifuged, and the supernatant was taken, and the supernatant was concentrated under reduced pressure to one seventh of the original volume, and a stabilizer was added, and the dosage ratio of honeysuckle pollen to stabilizer was controlled to 80g:40g, and stirred at room temperature at a stirring speed of 300rpm for 50min to obtain a first extract; 4. Prepare the second extract: wash the aloe leaves and cypress, take the aloe leaf pulp, dry the aloe leaf pulp and cypress, grind them to a particle size of 0.3 mm, and obtain aloe leaf pulp powder and cypress powder. Mix the aloe leaf pulp powder, cypress powder and distilled water in a dosage ratio of 100 g: 300 g: 700 mL, let stand at room temperature for 30 min, stir and reflux at 90 ° C at a stirring speed of 300 rpm for 4 h, cool naturally to room temperature, filter, take the filtrate, and use the filtrate as the second extract; 5. Mixing: Mix the first extract, the second extract, sodium hyaluronate, hydrolyzed hyaluronic acid, and the dispersant in a dosage ratio of 1200 mL: 700 mL: 40 g: 17 g: 80 g, and homogenize under high pressure at room temperature at a pressure of 30 MPa for 25 min to obtain a herbal hyaluronic acid pet ear wash solution; The molecular weight of the sodium hyaluronate is 50wDa; The molecular weight of the hydrolyzed hyaluronic acid is 10 kDa.

[0013] This embodiment also provides a herbal hyaluronic acid pet ear wash prepared by the aforementioned preparation method.

[0014] Example 2 A method for preparing a herbal hyaluronic acid pet ear wash liquid, specifically comprising: 1. Preparation of stabilizer: 205g bamboo charcoal powder and 3900mL distilled water were mixed, stirred at room temperature at a stirring speed of 400rpm for 55min, 8.8g sodium dodecylbenzene sulfonate was added, stirred at room temperature at a stirring speed of 400rpm for 35min, 5.1g cationic hyaluronic acid was added, stirred at room temperature at a stirring speed of 400rpm for 35min, 8.9g sodium dodecylbenzene sulfonate was added, stirred at room temperature at a stirring speed of 400rpm for 55min, filtered, rinsed the filter residue with distilled water, and dried to obtain a stabilizer; The particle size of the bamboo charcoal powder is 5 μm; The cationic hyaluronic acid has a number average molecular weight of 20 wDa and a degree of substitution of 0.15; 2. Preparation of dispersant: 51 g of soy protein and 6200 mL of distilled water were mixed, stirred at room temperature at a stirring speed of 400 rpm for 2.5 h, allowed to stand at 5 ° C for 22 h, 5% sodium hydroxide aqueous solution was added to adjust the pH to 12.2, stirred at room temperature at a stirring speed of 400 rpm for 55 min, 5% hydrochloric acid aqueous solution was added to adjust the pH to 7.2, stirred at room temperature at a stirring speed of 400 rpm for 55 min, then stirred at 60 ° C at a stirring speed of 400 rpm for 35 min, stirred at 70 ° C at a stirring speed of 400 rpm for 35 min, and stirred at 80 ° C for 35 min. Stirring at a stirring speed of 400 rpm for 35 min, stirring at a stirring speed of 400 rpm for 35 min at 90° C., transferring to an ice water bath and stirring at a stirring speed of 400 rpm, adding 150 mL of a 4% cationic hyaluronic acid aqueous solution and 72 mL of a 2% polyethylene glycol 2000 aqueous solution, stirring at a stirring speed of 400 rpm for 55 min in an ice water bath, adding 75 mL of a 5% sodium dodecylbenzene sulfonate aqueous solution, stirring at a stirring speed of 400 rpm for 55 min in an ice water bath, centrifuging, taking a precipitate, washing the precipitate with distilled water, and drying to obtain a dispersant; 3. Prepare the first extract: wash and dry honeysuckle, coptis chinensis, matricaria, sophora flavescens, witch hazel, dandelion leaf and mint respectively, grind them to a particle size of 0.3 mm to obtain honeysuckle powder, coptis chinensis powder, matricaria powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder and mint powder, then add honeysuckle powder, coptis chinensis powder, matricaria powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder, mint powder and distilled water in an amount ratio of 85g:75g:110g:85g:85g:1 45g:75g:9200mL were mixed, and allowed to stand at room temperature for 35min, stirred and refluxed at 92°C at a stirring speed of 350rpm for 6.5h, naturally cooled to room temperature, filtered, and the filtrate was taken, the filtrate was centrifuged, and the supernatant was taken, and the supernatant was concentrated under reduced pressure to one seventh of the original volume, and a stabilizer was added to control the dosage ratio of honeysuckle pollen to stabilizer to 85g:41g, and stirred at room temperature at a stirring speed of 350rpm for 55min to obtain a first extract; 4. Prepare the second extract: wash the aloe leaves and cypress, take the aloe leaf pulp, dry the aloe leaf pulp and cypress, grind them to a particle size of 0.3 mm, and obtain aloe leaf pulp powder and cypress powder. Mix the aloe leaf pulp powder, cypress powder and distilled water in a dosage ratio of 105g:320g:740mL, let stand at room temperature for 35min, stir and reflux at 92°C at a stirring speed of 350rpm for 4.5h, cool naturally to room temperature, filter, take the filtrate, and use the filtrate as the second extract; 5. Mixing: Mix the first extract, the second extract, sodium hyaluronate, hydrolyzed hyaluronic acid, and the dispersant in a ratio of 1230 mL: 720 mL: 42 g: 18 g: 83 g, and homogenize under high pressure at room temperature at a pressure of 33 MPa for 28 min to obtain a herbal hyaluronic acid pet ear wash solution; The molecular weight of the sodium hyaluronate is 50wDa; The molecular weight of the hydrolyzed hyaluronic acid is 10 kDa.

[0015] This embodiment also provides a herbal hyaluronic acid pet ear wash prepared by the aforementioned preparation method.

[0016] Example 3 A method for preparing a herbal hyaluronic acid pet ear wash liquid, specifically comprising: 1. Prepare a stabilizer: 210 g of bamboo charcoal powder and 4000 mL of distilled water were mixed, stirred at room temperature at a stirring speed of 500 rpm for 60 min, 9 g of sodium dodecylbenzene sulfonate was added, stirred at room temperature at a stirring speed of 500 rpm for 40 min, 5.2 g of cationic hyaluronic acid was added, stirred at room temperature at a stirring speed of 500 rpm for 40 min, 9 g of sodium dodecylbenzene sulfonate was added, stirred at room temperature at a stirring speed of 500 rpm for 60 min, filtered, rinsed the filter residue with distilled water, and dried to obtain a stabilizer; The particle size of the bamboo charcoal powder is 5 μm; The cationic hyaluronic acid has a number average molecular weight of 20 wDa and a degree of substitution of 0.15; 2. Preparation of dispersant: 52 g of soy protein and 6500 mL of distilled water were mixed, stirred at room temperature at a stirring speed of 500 rpm for 3 h, allowed to stand at 6 ° C for 25 h, 5% sodium hydroxide aqueous solution was added to adjust the pH to 12.5, stirred at room temperature at a stirring speed of 500 rpm for 60 min, 5% hydrochloric acid aqueous solution was added to adjust the pH to 7.5, stirred at room temperature at a stirring speed of 500 rpm for 60 min, then stirred at 62 ° C at a stirring speed of 500 rpm for 40 min, stirred at 72 ° C at a stirring speed of 500 rpm for 40 min, and stirred at 82 ° C at a stirring speed of 500 rpm for 40 min. The mixture was stirred at a stirring speed of 500 rpm for 40 min, stirred at a stirring speed of 500 rpm for 40 min at 92° C., transferred to an ice water bath and stirred at a stirring speed of 500 rpm, 160 mL of a 4% cationic hyaluronic acid aqueous solution and 75 mL of a 2% polyethylene glycol 2000 aqueous solution were added, stirred at a stirring speed of 500 rpm for 60 min in an ice water bath, 80 mL of a 5% sodium dodecylbenzene sulfonate aqueous solution were added, stirred at a stirring speed of 500 rpm for 60 min in an ice water bath, centrifuged, a precipitate was taken, the precipitate was rinsed with distilled water, and dried to obtain a dispersant; 3. Prepare the first extract: wash and dry honeysuckle, coptis chinensis, matricaria, sophora flavescens, witch hazel, dandelion leaf and mint respectively, grind them to a particle size of 0.3 mm to obtain honeysuckle powder, coptis chinensis powder, matricaria powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder and mint powder, then add honeysuckle powder, coptis chinensis powder, matricaria powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder, mint powder and distilled water in an amount ratio of 90g:80g:115g:90g:90g: 150g:80g:9500mL was mixed, and then allowed to stand at room temperature for 40min, stirred and refluxed at 95°C at a stirring speed of 400rpm for 7h, naturally cooled to room temperature, filtered, and the filtrate was taken, and the filtrate was centrifuged to take the supernatant, and the supernatant was concentrated under reduced pressure to one seventh of the original volume, and a stabilizer was added to control the dosage ratio of honeysuckle pollen to stabilizer to 90g:42g, and stirred at room temperature at a stirring speed of 400rpm for 60min to obtain a first extract; 4. Prepare the second extract: wash the aloe leaves and cypress, take the aloe leaf pulp, dry the aloe leaf pulp and cypress, grind them to a particle size of 0.3 mm, and obtain aloe leaf pulp powder and cypress powder. Mix the aloe leaf pulp powder, cypress powder and distilled water in a dosage ratio of 110 g: 340 g: 760 mL, let stand at room temperature for 40 min, stir and reflux at 95 ° C at a stirring speed of 400 rpm for 4.5 h, cool naturally to room temperature, filter, take the filtrate, and use the filtrate as the second extract; 5. Mixing: Mix the first extract, the second extract, sodium hyaluronate, hydrolyzed hyaluronic acid, and the dispersant in a ratio of 1250 mL: 750 mL: 43 g: 19 g: 85 g, and homogenize under high pressure at room temperature and a pressure of 35 MPa for 30 min to obtain a herbal hyaluronic acid pet ear wash solution; The molecular weight of the sodium hyaluronate is 50wDa; The molecular weight of the hydrolyzed hyaluronic acid is 10 kDa.

[0017] This embodiment also provides a herbal hyaluronic acid pet ear wash prepared by the aforementioned preparation method.

[0018] Comparative Example 1 The difference between this comparative example and Example 2 is that the first step of preparing the stabilizer is omitted, and in the third step of preparing the first extract, bamboo charcoal powder with a particle size of 5 μm is used instead of adding the stabilizer.

[0019] Comparative Example 2 The difference between this comparative example and Example 2 is that the dispersant preparation step in step 2 is omitted, and sodium dodecylbenzene sulfonate of equal mass is used instead of the dispersant in the mixing step in step 5.

[0020] Test Example 1 60 dogs and 60 cats with otitis were collected from a pet hospital in Qufu. The symptoms, age and physical condition of the 60 dogs and cats with otitis were guaranteed to be similar. The dogs and cats with otitis were randomly divided into six groups, each group including 10 dogs and 10 cats. For easy identification, they were marked as Groups 1-6. The herbal hyaluronic acid pet ear wash solution prepared in Examples 1-3 and Comparative Examples 1-2 were used in the treatment test of Groups 1-5, respectively, specifically, 1 mL of the herbal hyaluronic acid pet ear wash solution was dripped into the ear canal of the dog or cat every day, and massaged at the base of the ear for 30 seconds, and used continuously for 4 weeks. Group 6 was used as a blank control without using any ear wash solution; After the treatment trial, the recovery of otitis in each dog and cat in groups 1-6 was scored according to the following criteria: 8-10 points: Otitis symptoms completely disappeared or improved significantly; 5-7 points: Otitis symptoms improve and begin to get better; 0-4 points: no change or slight improvement in otitis symptoms; Then the scores of ear infection recovery in each group were averaged to obtain the average ear infection recovery score. The average ear infection recovery score was as follows:

[0021] After the treatment trial, the internal cleanliness of the ears of each dog and cat in Groups 1-6 was scored according to the following criteria: 8-10 points: There is no foreign body or almost no foreign body in the ear; 5-7 points: There is a foreign body inside the ear, but the foreign body is small; 0-4 points: There are many foreign objects in the ear; Then the scores of each group of internal ear cleaning were averaged to obtain the average cleaning score. The average internal cleaning scores obtained are as follows:

[0022] Test Example 2 According to the QB / T 2738-2012 test standard, the herbal hyaluronic acid pet ear wash prepared in Examples 1-3 and Comparative Examples 1-2 were tested for their bactericidal rates against Escherichia coli, Candida albicans, Staphylococcus aureus, and Pseudomonas aeruginosa. The test results are as follows:

[0023] Test Example 3 The herbal hyaluronic acid pet ear wash solution prepared in Example 1-3 and Comparative Example 1-2 was put into a packaging bottle, sealed and stored at room temperature for 6 months, and then the bactericidal rates of the herbal hyaluronic acid pet ear wash solution prepared in Example 1-3 and Comparative Example 1-2 against Escherichia coli, Candida albicans, Staphylococcus aureus, and Pseudomonas aeruginosa were tested according to the QB / T 2738-2012 test standard. The test results are as follows:

[0024] Test Example 4 The state of the herbal hyaluronic acid pet ear wash solution prepared in Example 1-3 and Comparative Example 1-2 was observed, and then subjected to freeze-thaw treatment, specifically, placed in an environment of -4°C for 24 hours, placed in an environment of 25°C for 12 hours, and then placed in an environment of -4°C for 24 hours. After being placed in an environment of 25°C for 12 hours, the appearance after freeze-thaw treatment was observed, and the observation results were as follows:

[0025] It can be seen from the above results that compared with the herbal hyaluronic acid pet ear wash liquid prepared in Example 2, the herbal hyaluronic acid pet ear wash liquid prepared in Comparative Example 1 has poor preparation effect on otitis and poor cleaning effect on the inside of the ear, a short shelf life and poor stability. The herbal hyaluronic acid pet ear wash liquid prepared in Comparative Example 2 has poor preparation effect on otitis and poor stability.

[0026] Unless otherwise specified, all percentages used in the present invention are by mass.

[0027] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing a herbal hyaluronic acid pet ear wash liquid, characterized in that: The method comprises the following steps: preparing a stabilizer, preparing a dispersant, preparing a first extracting solution, preparing a second extracting solution, and mixing; The method for preparing the stabilizer comprises mixing bamboo charcoal powder and distilled water, stirring at room temperature, adding sodium dodecylbenzene sulfonate, stirring at room temperature, adding cationic hyaluronic acid, stirring at room temperature, adding sodium dodecylbenzene sulfonate, stirring at room temperature, filtering, washing the filter residue with distilled water, and drying to obtain the stabilizer; The dispersant is prepared by mixing soybean protein and distilled water, stirring at room temperature, standing at 4-6°C, adding sodium hydroxide aqueous solution to adjust the pH to 12-12.5, stirring at room temperature, adding hydrochloric acid aqueous solution to adjust the pH to 7-7.5, stirring at room temperature, then stirring at 58-62°C, stirring at 68-72°C, stirring at 78-82°C, stirring at 88-92°C, transferring to an ice-water bath and starting stirring, adding cationic hyaluronic acid aqueous solution and polyethylene glycol 2000 aqueous solution, stirring in an ice-water bath, adding sodium dodecylbenzene sulfonate aqueous solution, stirring in an ice-water bath, centrifuging, taking a precipitate, washing the precipitate with distilled water, and drying to obtain a dispersant.

2. The method for preparing the herbal hyaluronic acid pet ear wash according to claim 1, characterized in that: In the preparation of the stabilizer, the mass ratio of bamboo charcoal powder, distilled water, sodium dodecylbenzene sulfonate, cationic hyaluronic acid, and sodium dodecylbenzene sulfonate is 200-210 g: 3700-4000 mL: 8.7-9 g: 5-5.2 g: 8.7-9 g; The particle size of the bamboo charcoal powder is 5 μm; The number average molecular weight of the cationic hyaluronic acid is 20 wDa, and the degree of substitution is 0.

15.

3. The method for preparing the herbal hyaluronic acid pet ear wash according to claim 1, characterized in that: In the preparation of the dispersant, the mass volume ratio of soybean protein, distilled water, cationic hyaluronic acid aqueous solution, polyethylene glycol 2000 aqueous solution, and sodium dodecylbenzene sulfonate aqueous solution is 50-52g:6000-6500mL:140-160mL:70-75mL:70-80mL; The mass concentration of the sodium hydroxide aqueous solution is 5%; The mass concentration of the hydrochloric acid aqueous solution is 5%; The mass concentration of the cationic hyaluronic acid aqueous solution is 4%; The mass concentration of the polyethylene glycol 2000 aqueous solution is 2%; The mass concentration of the sodium dodecylbenzenesulfonate aqueous solution is 5%.

4. The method for preparing the herbal hyaluronic acid pet ear wash according to claim 1, characterized in that: In the method for preparing the first extract, honeysuckle, coptis root, matricaria, sophora flavescens, witch hazel, dandelion leaf and mint are washed and dried respectively, and then ground to obtain honeysuckle pollen, coptis root powder, matricaria powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder and mint powder; then the honeysuckle pollen, coptis root powder, matricaria powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder, mint powder and distilled water are mixed, and the mixture is allowed to stand at room temperature, stirred and refluxed at 90-95° C., naturally cooled to room temperature, filtered, the filtrate is taken, the filtrate is centrifuged, the supernatant is taken, the supernatant is concentrated under reduced pressure to one seventh of the original volume, a stabilizer is added, and the mixture is stirred at room temperature to obtain the first extract.

5. The method for preparing the herbal hyaluronic acid pet ear wash according to claim 4, characterized in that: In the preparation of the first extract, the honeysuckle powder, coptis powder, chamomile powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder, mint powder, and distilled water are used in a ratio of 80-90g:70-80g:100-115g:80-90g:80-90g:140-150g:70-80g:9000-9500mL; The dosage ratio of honeysuckle powder to stabilizer is 80-90g:40-42g; The particle size of honeysuckle powder, coptis powder, chamomile powder, sophora flavescens powder, witch hazel powder, dandelion leaf powder and mint powder is 0.3 mm.

6. The method for preparing the herbal hyaluronic acid pet ear wash according to claim 1, characterized in that: The second extract is prepared by washing aloe leaves and cypress separately, taking aloe leaf pulp, drying the aloe leaf pulp and cypress, grinding them to obtain aloe leaf pulp powder and cypress powder, mixing the aloe leaf pulp powder, cypress powder and distilled water, leaving them to stand at room temperature, stirring and refluxing at 90-95°C, naturally cooling to room temperature, filtering, taking the filtrate, and using the filtrate as the second extract.

7. The method for preparing the herbal hyaluronic acid pet ear wash according to claim 6, characterized in that: In the preparation of the second extract, the ratio of aloe leaf powder, cypress powder and distilled water is 100-110 g: 300-340 g: 700-760 mL; The particle size of the aloe leaf powder and the cypress powder is 0.3 mm.

8. The method for preparing the herbal hyaluronic acid pet ear wash according to claim 1, characterized in that: The mixing comprises mixing the first extract, the second extract, sodium hyaluronate, hydrolyzed hyaluronic acid and the dispersant, and then performing high pressure homogenization to obtain a herbal hyaluronic acid pet ear wash solution.

9. The method for preparing the herbal hyaluronic acid pet ear wash according to claim 8, characterized in that: In the mixing, the dosage ratio of the first extract, the second extract, sodium hyaluronate, hydrolyzed hyaluronic acid, and the dispersant is 1200-1250 mL: 700-750 mL: 40-43 g: 17-19 g: 80-85 g; The molecular weight of the sodium hyaluronate is 50wDa; The molecular weight of the hydrolyzed hyaluronic acid is 10 kDa; The pressure of the high-pressure homogenization is 30-35 MPa, and the time is 25-30 min.

10. A herbal hyaluronic acid pet ear wash prepared by the preparation method according to any one of claims 1 to 9.

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