Microbial fermentation culture device and method for antibiotic preparation

Abstract

The invention discloses a microbial fermentation culture device and method for antibiotic preparation, and aims to improve the feeding precision of antibiotic raw materials and the stability of a culture environment. The device comprises a fixed base, a direction shaft, a culture machine shell, a heating plate, a cooling sleeve, a feeding table and a feeding mechanism. The feeding mechanism is driven by a servo motor, and automatic conveying of raw materials is achieved. A stepping motor and a stirring paddle are arranged in the culture machine shell and used for stirring raw materials. The device is further provided with a temperature sensor, a dosing pipeline and a cooling medium circulating system, so that culture conditions are accurately controlled. The using method comprises the four steps of material feeding, inoculation, culture and antibiotic generation, and the production efficiency and the product quality are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of antibiotics, in particular to a microbial fermentation culture device and method for preparing antibiotics. Background Art

[0002] Antibiotics are a class of drugs widely used in the medical and veterinary fields to prevent or treat bacterial infections. Different antibiotics have different chemical structures, and the methods of producing antibiotics are therefore also different. Among them, fermentation culture equipment can provide a suitable growth and development environment for biological living cells or enzymes, enabling them to proliferate and produce biological cells.

[0003] The microbial fermentation and culture device can provide a good biological reaction environment. The multi-parameter control system can regulate the temperature, pressure, gas composition, pH value, ion concentration and other parameters in the reaction process, so as to ensure that the microorganisms carry out metabolic reactions in a good growth environment. The microbial fermentation and culture device can provide the necessary growth matrix and nutrients to promote the metabolic reactions of microorganisms and control the generation rate and type of metabolites to a certain extent. The reaction conditions can also be optimized by controlling parameters such as stirring speed, ventilation rate and fluidity in the tank to promote microbial metabolic reactions. The microbial fermentation and culture device can effectively increase the content and purity of microbial metabolites. Different centrifugation, ultrafiltration, gel filtration and other technologies can be used in the tank to remove microbial cells and impurities and improve product purity. At the same time, the products in the reaction can also be quantitatively and qualitatively analyzed to optimize the reaction conditions and improve the product yield.

[0004] However, the traditional fermentation culture device has the following disadvantages:

[0005] In the process of preparing antibiotic raw materials, traditional fermentation and culture devices mainly rely on manual operation to inject raw materials through pipelines. Manual feeding requires precise control of the amount and time of raw materials, but it is often difficult to achieve the ideal accuracy in actual operation, which can easily lead to uneven feeding or errors, affecting the fermentation effect. Traditional fermentation and culture devices have limitations in heating and cooling methods, and it is difficult to quickly respond to temperature changes, resulting in an unstable culture environment. Summary of the invention

[0006] The object of the present invention is to provide a microbial fermentation culture device and method for preparing antibiotics to solve the problems raised in the above background technology.

[0007] To achieve the above object, the present invention provides the following technical solution: a microbial fermentation and culture device for preparing antibiotics, comprising:

[0008] Fixed base;

[0009] A direction axis is rotatably connected to the top of the fixed base to adjust the direction of the culture housing;

[0010] The culture housing is fixedly mounted on the top of the directional axis and serves as a culture environment for microbial fermentation;

[0011] The heating plate is fixedly installed inside the culture housing to provide the temperature required by the culture environment;

[0012] A cooling jacket is fixedly connected to the middle of the culture housing and adjusts the temperature outside the culture housing through a cooling medium;

[0013] The feed table is fixedly installed in the middle of the side of the culture housing and serves as an auxiliary structure for raw material input;

[0014] The feeding mechanism is fixedly installed on the top of the feeding platform, and comprises a raw material shell and a conveying shell, the sides of which are fixedly connected to convey the antibiotic raw materials into the culture machine shell.

[0015] Furthermore, the feeding mechanism also includes:

[0016] The servo motor is fixedly mounted on the side of the top of the raw material shell to drive the entire feeding process;

[0017] The rotating shaft, driven by a servo motor, passes through the limit plate and drives the subsequent components to rotate;

[0018] A turntable, which is connected to the rotating shaft through a mounting shaft and a direction plate, and drives the deflection of the direction plate;

[0019] The sealing pad slides inside the conveying shell, generating attraction to extract the raw materials when sliding;

[0020] The fixing frame and the direction seat are fixedly connected with the sealing gasket, so that the sliding direction of the sealing gasket can be controlled;

[0021] The active umbrella-shaped bevel gear and the driven umbrella-shaped bevel gear are respectively mounted on the rotating shaft and the first stirring paddle, and drive stirring through meshing;

[0022] A conveying pipeline, connecting the conveying shell and the culture shell;

[0023] An extraction pipeline connecting the raw material shell and the conveying shell;

[0024] Feed pipe, adding antibiotic raw materials into the raw material shell.

[0025] Furthermore, the side of the conveying shell away from the raw material shell is fixedly connected to a conveying pipe extending to the interior of the culture shell, the bottom end of the side of the raw material shell is fixedly connected to an extraction pipe extending to the interior of the conveying shell, and the bottom end of the raw material shell is fixedly connected to the feeding table to realize automatic conveying of the raw materials.

[0026] Furthermore, a stepper motor is fixedly mounted in the middle of the top of the culture housing, and a second stirring paddle located inside the culture housing is fixedly mounted at the output end of the stepper motor for stirring the raw materials during the culture process.

[0027] Furthermore, a drug adding pipeline is fixedly connected to the side of the top of the culture casing for adding necessary drugs or gases; a temperature sensor extending to the inside of the culture casing is fixedly installed on the other side of the top of the culture casing for real-time monitoring of the temperature inside the culture casing.

[0028] Furthermore, a gear body is fixedly installed in the middle of the direction axis, a positioning plate is fixedly installed on the side of the top of the fixed base away from the direction axis, a pushing cylinder is fixedly installed in the middle of the positioning plate, a movable plate is fixedly installed on the movable end of the pushing cylinder, the outer side of the gear body is meshed and connected with a plurality of teeth fixedly arranged on the side of the movable plate, and a movable block slidably connected to the fixed base is fixedly installed at the bottom end of the movable plate, and the rotation of the culture casing is realized by driving the pushing cylinder.

[0029] Furthermore, the liquid inlet of the cooling jacket is fixedly connected to a liquid inlet pipe, and the liquid outlet of the cooling jacket is fixedly connected to a liquid discharge pipe, which are used for the circulation of cooling medium and the adjustment of the temperature outside the culture casing.

[0030] The method for using the microbial fermentation culture device for preparing antibiotics comprises the following steps:

[0031] Step 1: Material feeding: injecting the prepared antibiotic raw material into the culture housing through the feeding mechanism, and introducing air to increase oxygen;

[0032] Step 1: Inoculation of materials: inoculating the screened microorganisms for preparing antibiotics into a culture housing;

[0033] Step 1: Cultivate the material, maintain a certain temperature in the cultivation shell for 12-24 hours, during which the temperature sensor displays the temperature in real time, and the heating plate or cooling jacket heats or cools as needed;

[0034] Step 1: Produce antibiotics. The target antibiotics are continuously produced in the culture housing for 3-5 days.

[0035] Compared with the prior art, the present invention has the following beneficial effects:

[0036] 1. The sealing gasket of the feeding mechanism of the present invention slides along the conveying shell to generate attraction, so that the extraction pipeline extracts the raw materials for antibiotic culture stored in the raw material shell. The extracted raw materials are transported to the culture shell along the conveying pipeline as the sealing gasket slides down, replacing the traditional manual feeding, and the operation is simple and convenient;

[0037] 2. The present invention is provided with a heating plate cooling jacket. When the heating wire in the heating plate is energized, it generates heat to heat the antibiotic raw materials in the culture casing, or the condensate in the cooling jacket is cooled from the outside of the culture casing, so that the culture casing can cultivate the raw materials in the culture casing at a fixed temperature. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 is a cross-sectional view of the present invention;

[0039] Figure 2 It is a partial schematic diagram of the present invention;

[0040] Figure 3 It is a connection diagram of the culture housing and the heating plate of the present invention;

[0041] Figure 4 is a cross-sectional view of the feeding mechanism of the present invention;

[0042] Figure 5 It is a partial schematic diagram of the feeding mechanism of the present invention;

[0043] Figure 6 It is a side view of the feeding mechanism of the present invention;

[0044] Figure 7 It is a flow chart of the present invention.

[0045] In the figure: 1, fixed base; 2, gear body; 3, movable block; 4, positioning plate; 5, push cylinder; 6, movable plate; 7, direction shaft; 8, culture housing; 9, temperature sensor; 10, stepper motor; 11, dosing pipeline; 12, feeding mechanism; 1201, raw material housing; 1202, feeding pipeline; 1203, servo motor; 1204, active umbrella-shaped helical gear; 1205, rotating shaft; 1206, turntable; 1207 , limit plate; 1208, mounting shaft; 1209, direction plate; 1210, direction seat; 1211, fixing frame; 1212, conveying pipe; 1213, sealing gasket; 1214, extraction pipe; 1215, conveying shell; 1216, first stirring paddle; 1217, driven umbrella-shaped bevel gear; 13, feeding table; 14, cooling jacket; 15, discharge pipe; 16, inlet pipe; 17, second stirring paddle; 18, heating plate. DETAILED DESCRIPTION

[0046] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0047] See also Figure 1-Figure 7 The present invention provides a microbial fermentation and culture device for preparing antibiotics, comprising a fixed base 1, the side portion of the top of the fixed base 1 is rotatably connected with a direction shaft 7, the top of the direction shaft 7 is fixedly installed with a culture casing 8, the interior of the culture casing 8 is fixedly installed with a heating plate 18, the middle of the culture casing 8 is fixedly connected with a cooling jacket 14, the middle of the side of the culture casing 8 is fixedly installed with a feed table 13, the top of the feed table 13 is fixedly installed with a feed mechanism 12, the feed mechanism 12 comprises a raw material shell 1201 and a conveying shell 1215, the side of the raw material shell 1201 is fixedly connected with the side of the conveying shell 1215, the heating plate 18 is started after being energized, the heating wire in the heating plate 18 is heated after being energized, and the heating plate 18 is heated from inside the culture casing 8.

[0048] A servo motor 1203 is fixedly installed on the side of the top of the raw material shell 1201, a limit plate 1207 is fixedly installed on the other side of the top of the raw material shell 1201, a rotating shaft 1205 that passes through the limit plate 1207 is fixedly installed on the output end of the servo motor 1203, a turntable 1206 is fixedly installed on one end of the rotating shaft 1205, a mounting shaft 1208 is fixedly installed on one end of the turntable 1206, a direction plate 1209 is installed in the middle of the mounting shaft 1208, a sealing gasket 1213 is slidably connected to the inside of the conveying shell 1215, and a sealing gasket 1213 is fixedly installed on the top of the sealing gasket A fixed frame 1211, a direction seat 1210 is fixedly installed on the top of the fixed frame 1211, the interior of the direction seat 1210 is rotatably connected to the side opposite to the direction plate 1209, an active umbrella-shaped bevel gear 1204 is fixedly installed on the middle of the rotating shaft 1205, a first stirring paddle 1216 is rotatably connected to the top of the inner wall of the conveying shell 1215, a driven umbrella-shaped bevel gear 1217 is fixedly installed on the top of the first stirring paddle 1216, the outer side of the active umbrella-shaped bevel gear 1204 is meshedly connected with the outer side of the driven umbrella-shaped bevel gear 1217, and the servo motor 1203 is started after being energized.

[0049] The servo motor 1203 drives the rotating shaft 1205 to rotate, and the rotating shaft 1205 drives the active umbrella-shaped bevel gear 1204 to rotate. The active umbrella-shaped bevel gear 1204 contacts the driven umbrella-shaped bevel gear 1217, and the driven umbrella-shaped bevel gear 1217 drives the first stirring paddle 1216 to rotate, so as to stir the antibiotic raw material in the raw material shell 1201. After the rotating shaft 1205 rotates, it drives the installation shaft 1208 to rotate, and then drives the direction plate 1209 to move synchronously. The direction plate 1209 is angularly deflected relative to the direction seat 1210, and the sealing gasket 1213 slides relative to the conveying shell 1215. When the sealing gasket 1213 slides, an attraction force is generated, so that the extraction pipe 1214 extracts the raw material in the raw material shell 1201.

[0050] The side of the conveying shell 1215 away from the raw material shell 1201 is fixedly connected to a conveying pipe 1212 extending to the interior of the culture shell 8, the bottom end of the side of the raw material shell 1201 is fixedly connected to an extraction pipe 1214 extending to the interior of the conveying shell 1215, the surface of the raw material shell 1201 is fixedly connected to a feeding pipe 1202, and the bottom end of the raw material shell 1201 is fixedly connected to the feeding platform 13. The prepared antibiotic raw materials are injected into the raw material shell 1201 through the feeding pipe 1202, and the raw materials in the raw material shell 1201 are conveyed to the conveying shell 1215 through the extraction pipe 1214, and are conveyed to the culture shell 8 through the conveying pipe 1212.

[0051] A stepper motor 10 is fixedly installed in the middle of the top of the culture shell 8, and a second stirring paddle 17 located inside the culture shell 8 is fixedly installed at the output end of the stepper motor 10. The stepper motor 10 starts after being powered on, and the stepper motor 10 drives the second stirring paddle 17 to rotate, and the second stirring paddle 17 stirs the raw materials in the culture shell 8.

[0052] The side of the top of the culture shell 8 is fixedly connected to a dosing pipe 11, and the other side of the top of the culture shell 8 is fixedly installed with a temperature sensor 9 extending to the inside of the culture shell 8. The temperature sensor 9 senses the temperature inside the culture shell 8 in real time, and the gas and drugs required for fermentation culture are injected into the culture shell 8 through the dosing pipe 11.

[0053] A gear body 2 is fixedly installed in the middle of the direction shaft 7, a positioning plate 4 is fixedly installed on the side of the top of the fixed base 1 away from the direction shaft 7, a pushing cylinder 5 is fixedly installed in the middle of the positioning plate 4, and a movable plate 6 is fixedly installed on the movable end of the pushing cylinder 5. The outer side of the gear body 2 is meshed and connected with a plurality of teeth fixedly arranged on the side of the movable plate 6, and a movable block 3 slidingly connected with the fixed base 1 is fixedly installed on the bottom end of the movable plate 6. The pushing cylinder 5 performs telescopic and deflection movements, and the pushing cylinder 5 pushes the movable plate 6 from the side. A plurality of teeth fixedly arranged on the side of the movable plate 6 contact with the teeth on the outer side of the gear body 2. The movable plate 6 is driven by friction to rotate the gear body 2, and the gear body 2 drives the direction shaft 7 to move synchronously. The direction shaft 7 drives the culture casing 8 to move synchronously to stir the antibiotic raw materials prepared in the culture casing 8.

[0054] The liquid inlet of the cooling jacket 14 is fixedly connected to a liquid inlet pipe 16, and the liquid outlet of the cooling jacket 14 is fixedly connected to a liquid discharge pipe 15. The cooling liquid is injected into the cooling jacket 14 through the liquid inlet pipe 16. The cooling jacket 14 is cooled from the outside of the culture shell 8, and the cooling liquid is discharged to the outside through the liquid discharge pipe 15.

[0055] The method for using the microbial fermentation culture device for preparing antibiotics of the present invention comprises the following steps:

[0056] Step 1: Material feeding: The prepared antibiotics are injected into the culture housing 8 through the feeding mechanism 12, and air is introduced to increase oxygen;

[0057] Step 2, material inoculation: the microorganisms for preparing antibiotics screened by the screening device are inoculated into the culture housing 8;

[0058] Step 3, material culture: maintain a certain temperature in the culture housing 8 for 12-24 hours, the temperature sensor 9 displays the temperature in the culture housing 8 in real time, the heating plate 18 heats the antibiotic raw material in the culture housing 8 or the cooling jacket 14 cools the antibiotic raw material from the outside of the culture housing 8 to form bacteria;

[0059] Step 4: Produce antibiotics: The culture housing 8 produces target antibiotics for a duration of 3-5 days.

[0060] The servo motor 1203 of the present invention is started after being powered on, and the servo motor 1203 drives the rotating shaft 1205 to rotate, and the rotating shaft 1205 drives the active umbrella-shaped bevel gear 1204 to rotate, and the active umbrella-shaped bevel gear 1204 contacts the driven umbrella-shaped bevel gear 1217, and the driven umbrella-shaped bevel gear 1217 drives the first stirring paddle 1216 to rotate, so as to stir the antibiotic raw material in the raw material shell 1201, and the rotating shaft 1205 rotates and drives the installation shaft 1208 to rotate and then drives the direction plate 1209 to move synchronously, and the direction plate 1209 is deflected at an angle relative to the direction seat 1210, and the sealing gasket 1213 slides relative to the conveying shell 1215, and when the sealing gasket 1213 slides An attractive force is generated, so that the extraction pipe 1214 extracts the raw material in the raw material shell 1201, and the raw material for preparing antibiotics is injected into the raw material shell 1201 through the feed pipe 1202. The raw material in the raw material shell 1201 is transported to the conveying shell 1215 through the extraction pipe 1214, and is transported to the culture shell 8 through the conveying pipe 1212. The cooling liquid is injected into the cooling jacket 14 through the liquid inlet pipe 16. The cooling jacket 14 cools down from the outside of the culture shell 8. The heating plate 18 is started after being energized. The heating wire in the heating plate 18 heats up after being energized. The heating plate 18 is heated from inside the culture shell 8, and the microorganisms for preparing antibiotics screened by the screening equipment are connected to the culture shell 8.

[0061] A certain temperature is maintained in the culture casing 8 for 12-24 hours, the temperature sensor 9 displays the temperature in the culture casing 8 in real time, the heating plate 18 heats the antibiotic raw material in the culture casing 8 or the cooling sleeve 14 cools the antibiotic raw material from the outside of the culture casing 8 to form bacteria; the culture casing 8 produces the target antibiotic for a duration of 3-5 days.

[0062] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A microbial fermentation and culture device for preparing antibiotics, characterized in that: It includes: A fixed base (1); A direction shaft (7) is rotatably connected to the top of the fixed base (1) to adjust the direction of the culture housing; A culture housing (8) is fixedly mounted on the top of the directional shaft (7) and serves as a culture environment for microbial fermentation; A heating plate (18) is fixedly installed inside the culture housing (8) to provide a temperature required by the culture environment; A cooling jacket (14) is fixedly connected to the middle of the culture housing (8) and adjusts the temperature outside the culture housing (8) through a cooling medium; A feed table (13) is fixedly mounted in the middle of the side of the culture housing (8) and serves as an auxiliary structure for raw material input; The feeding mechanism (12) is fixedly mounted on the top of the feeding platform (13), and comprises a raw material shell (1201) and a conveying shell (1215), the sides of which are fixedly connected to convey the antibiotic raw material into the culture machine shell (8).

2. The microbial fermentation and culture device for preparing antibiotics according to claim 1, characterized in that: The feeding mechanism (12) further comprises: A servo motor (1203) is fixedly mounted on the side of the top of the raw material shell (1201) and is used to drive the entire feeding process; The rotating shaft (1205) is driven by the servo motor (1203), passes through the limit plate (1207), and drives the subsequent components to rotate; A rotating disk (1206), the rotating disk (1206) is connected to the rotating shaft (1205) through a mounting shaft (1208) and a direction plate (1209), and drives the deflection of the direction plate; The sealing pad (1213) slides inside the conveying shell (1215) and generates attraction to extract the raw materials when sliding; The fixing frame (1211) and the direction seat (1210) are fixedly connected to the sealing gasket (1213), so that the sliding direction of the sealing gasket can be controlled; The active bevel gear (1204) and the driven bevel gear (1217) are respectively mounted on the rotating shaft (1205) and the first stirring paddle (1216), and are driven to stir by meshing; A conveying pipeline (1212) connecting the conveying shell (1215) and the culture shell (8); An extraction pipeline (1214) connecting the raw material shell (1201) and the conveying shell (1215); The feed pipe (1202) adds the antibiotic raw material into the raw material shell (1201).

3. The microbial fermentation and culture device for preparing antibiotics according to claim 1, characterized in that: The side of the conveying shell (1215) away from the raw material shell (1201) is fixedly connected to a conveying pipe (1212) extending to the interior of the culture machine shell (8), and the bottom end of the side of the raw material shell (1201) is fixedly connected to an extraction pipe (1214) extending to the interior of the conveying shell (1215). The bottom end of the raw material shell (1201) is fixedly connected to the feeding platform (13) to realize automatic conveying of the raw materials.

4. The microbial fermentation and culture device for preparing antibiotics according to claim 1, characterized in that: A stepper motor (10) is fixedly mounted in the middle of the top of the culture housing (8), and a second stirring paddle (17) located inside the culture housing (8) is fixedly mounted at the output end of the stepper motor (10) for stirring the raw materials during the culture process.

5. The microbial fermentation and culture device for preparing antibiotics according to claim 1, characterized in that: The side portion of the top of the culture housing (8) is fixedly connected to a drug adding pipeline (11) for adding necessary drugs or gases; the other side of the top of the culture housing (8) is fixedly installed with a temperature sensor (9) extending into the interior of the culture housing (8) for real-time monitoring of the temperature inside the culture housing.

6. The microbial fermentation and culture device for preparing antibiotics according to claim 1, characterized in that: A gear body (2) is fixedly installed in the middle of the direction shaft (7); a positioning plate (4) is fixedly installed on the side of the top of the fixed base (1) away from the direction shaft (7); a pushing cylinder (5) is fixedly installed in the middle of the positioning plate (4); a movable plate (6) is fixedly installed on the movable end of the pushing cylinder (5); the outer side of the gear body (2) is meshed with a plurality of teeth fixedly arranged on the side of the movable plate (6); a movable block (3) slidably connected to the fixed base (1) is fixedly installed at the bottom end of the movable plate (6); and the rotation of the culture casing is achieved by driving the pushing cylinder.

7. The microbial fermentation and culture device for preparing antibiotics according to claim 1, characterized in that: The liquid inlet of the cooling jacket (14) is fixedly connected to a liquid inlet pipeline (16), and the liquid outlet of the cooling jacket (14) is fixedly connected to a liquid discharge pipeline (15), which are used for the circulation of cooling medium and the adjustment of the temperature outside the culture housing (8).

8. The method for using the microbial fermentation culture device for preparing antibiotics according to any one of claims 1 to 7, comprising the following steps: Step 1: Material feeding: injecting the prepared antibiotic raw material into the culture housing (8) through the feeding mechanism (12), and introducing air to increase oxygen; Step 1: inoculating materials, inoculating the screened microorganisms for preparing antibiotics into a culture housing (8); Step 1: Cultivate the material, and maintain a certain temperature in the cultivation housing (8) for 12-24 hours, during which the temperature sensor (9) displays the temperature in real time, and the heating plate (18) or the cooling jacket (14) performs heating or cooling as required; Step 1: Produce antibiotics. The target antibiotics are continuously produced in the culture housing (8) for a period of 3-5 days.

Images