Liquid-phase fluorescence detector

Abstract

The invention relates to a liquid-phase fluorescence detector, which solves the problems of incapability of detecting ultraviolet fluorescent substances, detection accuracy, automatic detection degree and efficiency. According to the technical scheme, a constant-temperature sample incubation support, a constant-temperature detection agent incubation support, a constant-temperature sample detection support and a fluorescence detection head part moving longitudinally and transversely are arranged on a base, and a light source light path of an LED detection light source and a detection light path provided with a fluorescence detection sensor are arranged on a detection head body. A sample cup used for detection is characterized in that the cup bottom is a plane, the cup body is conical, and the sample cup is made of a high-light-transmittance GPPS (General Purpose Polystyrene) material. The invention has the advantages of wide range of detected fluorescent substances, implementation of batch detection of samples, automatic control of the whole detection process, high detection efficiency, accurate and reliable detection results, mutual verification, low cost and the like, and can be widely used for medical detection, chemical substance analysis and the like.

Description

Technical Field

[0001] The invention belongs to an inspection instrument based on fluorescent marking for detecting and analyzing chemical substances, in particular to a liquid phase fluorescence detector used for medical examination. Background Art

[0002] The fluorescence detectors used in the prior art (such as dry fluorescence analyzers, real-time nucleic acid amplification analyzers, and constant temperature nucleic acid amplification analyzers) all use laser light excitation wavelengths in the blue and green bands. Due to the low cost of blue and green laser lights, they have been widely used in nucleic acid amplification analyzers and constant temperature nucleic acid amplification analyzers in the prior art, but the fluorescence detector can only detect fluorescent substances in the blue and green bands. Due to the high cost of ultraviolet laser lights and the large size of ultraviolet laser lights, the large-scale application of ultraviolet laser lights in fluorescence analyzers is limited.

[0003] Although LED ultraviolet lamps have advantages such as a wide wavelength range and low cost, even if an OD6 filter is used for LED ultraviolet lamps, there are still 422nm and other bands of emitted light (detected light) after filtering. These detected light bands that cannot be completely filtered reflect seriously during the ultraviolet light test, resulting in inaccurate test values. For example, when a dry fluorescence analyzer uses a 365nm band (ultraviolet light) to test card-type detection reagents, the ultraviolet light not only irradiates the fluorescent substance, but also irradiates the card detection box. The light reflected by the card detection box causes a large deviation in the test value. In the prior art, the light source of the real-time nucleic acid amplification analyzer and the constant temperature nucleic acid amplification analyzer irradiates the sample cup from the top. Due to the different liquid levels in the sample cup, the ultraviolet light cannot always be focused on the liquid surface, and the test cannot be carried out. The sample cup of the prior art is made of PP material, which has strong reflection of ultraviolet light and large reflection interference; the fluorescent substance is at the bottom, and the distance between the fluorescent substance and the light source is far, the reflection interference is large, and the test results are inaccurate. In summary, the liquid phase fluorescence detector publicly used in the prior art is still unable to detect ultraviolet fluorescent substances (ultraviolet fluorescent substances include fluorescent brightener 28, AMCA, AMCA-X, ABQ, DAPI, Acridline, Alexa, Fluor350, Fluor405, BFP, Calcein, Violet, Calcofluor White, Dansyl, DIFUM, FAST BLUE, Flourspheres Blue, hoechst33258, hoechst33342, Pacific Blue, PBFI, Y66H, True Blue, etc.), which greatly affects the detection and use scope of the liquid phase fluorescence detector. Summary of the invention

[0004] In order to solve the technical problems of reducing the cost of ultraviolet light source, increasing the detection of ultraviolet fluorescent substances, improving the detection accuracy and the degree of automated detection and efficiency of existing fluorescence detectors, the present invention discloses a liquid phase fluorescence detector.

[0005] The technical solution adopted by the present invention to achieve the purpose of the invention is: a liquid phase fluorescence detector, including: an inspection instrument base, an inspection instrument upper cover, a fluorescence detection head part, a detection head movement control part and a sample cup.

[0006] The base of the inspection instrument is provided with a constant temperature sample incubation bracket, a constant temperature detection agent incubation bracket and a constant temperature sample detection bracket. The constant temperature sample incubation bracket and the constant temperature sample detection bracket are respectively provided with a group of sample cup accommodating holes for being detected, and the constant temperature detection agent incubation bracket is provided with a group of sample cup accommodating holes for reagent incubation.

[0007] The fluorescence detection head part comprises: a detection head body, an LED detection light source, a light source flat lens, a sampling end flat lens, a detection end flat lens, a light source filter, a detection filter, a dichroic mirror and a fluorescence detection sensor.

[0008] On the detection head body, the light source optical path is composed of an LED detection light source, a light source flat lens, a light source filter, a dichroic mirror and a sampling end flat lens; the detection optical path is composed of a sampling end flat lens, a dichroic mirror, a detection filter and a detection end flat lens; the receiving end of the fluorescence detection sensor is connected to the detection end flat lens of the detection optical path.

[0009] The fluorescent detection head part is connected to the sliding frame in a longitudinally controllable movement through the detection head body and the longitudinal transmission part, and the sliding frame and the guide rail transverse transmission part are connected to the inspection instrument base in a transversely controllable movement.

[0010] The upper cover of the inspection instrument is provided with operation doors corresponding to the sample cup receiving hole and the reagent incubation cup receiving hole.

[0011] The sample cup has a flat bottom and a conical body, and is made of a light-transmitting material.

[0012] The beneficial effects of the present invention are: a wide range of fluorescent substances can be detected, batch detection of samples can be implemented, the detection process is fully automatically controlled, the detection efficiency is high, the detection results are accurate and reliable, and the cost is low. It can be widely used in medical detection and analysis of chemical substances.

[0013] The present invention is described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Attached Figure 1 It is a schematic diagram of the disassembly of the inspection instrument base and the inspection instrument upper cover of the present invention.

[0015] Attached Figure 2 It is a schematic diagram of the structure of the detection head part of the present invention.

[0016] Attached Figure 3 For attachment Figure 2 Schematic diagram of the A-direction structure.

[0017] In the attached drawings, 1. inspection instrument base, 2. constant temperature sample incubation bracket, 3. constant temperature detection agent incubation bracket, 4. constant temperature sample detection bracket, 5. detection sample cup receiving hole, 6. reagent incubation sample cup receiving hole, 7. detection head, 8. LED detection light source, 9-1. light source flat lens, 9-2. sampling end flat lens, 9-3. detection end flat lens, 10-1. light source filter, 10-2. detection filter, 11. dichroic mirror, 12. fluorescence detection sensor, 13. sliding rack, 14. guide rail, 15. inspection instrument cover, 16. operation cabin door, 17. sample cup, 18. heating plate. DETAILED DESCRIPTION

[0018] A liquid phase fluorescence detector comprises: an inspection instrument base, an inspection instrument upper cover, a fluorescence detection head part, a detection head movement control part and a sample cup.

[0019] The inspection instrument base 1 is provided with a constant temperature sample incubation bracket 2, a constant temperature detection agent incubation bracket 3 and a constant temperature sample detection bracket 4. The constant temperature sample incubation bracket 2 and the constant temperature sample detection bracket 4 are respectively provided with a group of sample cup accommodating holes 5 for being detected, and the constant temperature detection agent incubation bracket 3 is provided with a group of sample cup accommodating holes 6 for reagent incubation.

[0020] The sample cup 17 in the sample cup accommodating hole 5 on the constant temperature sample incubation support 2 and the constant temperature sample detection support 4 is used for sample heating, incubation and detection.

[0021] The sample cup 17 in the sample cup receiving hole 6 on the constant temperature detection agent incubation bracket 3 is used for detection agent incubation.

[0022] The fluorescence detection head part includes: a detection head body 7, an LED detection light source 8, a light source flat lens 9-1, a sampling end flat lens 9-2, a detection end flat lens 9-3, a light source filter 10-1, a detection filter 10-2, a dichroic mirror 11 and a fluorescence detection sensor 12.

[0023] On the detection head body 7, the light source optical path is composed of the LED detection light source 8, the light source flat lens 9-1, the light source filter 10-1, the dichroic mirror 11 and the sampling end flat lens 9-2, and the detection optical path is composed of the sampling end flat lens 9-2, the dichroic mirror 11, the detection filter 10-2 and the detection end flat lens 9-3. The light receiving end of the fluorescence detection sensor 12 is connected to the detection optical path.

[0024] When in use, the sampling end flat lens 9 - 2 corresponds to the bottom surface of the sample cup 17 on the constant temperature sample detection bracket 4 , and the detection end flat lens 9 - 3 corresponds to the light receiving end of the fluorescence detection sensor 12 .

[0025] The LED detection light source 8 is an ultraviolet lamp, and the ultraviolet light emitted is transmitted through the light source flat lens 9-1, filtered by the light source filter 10-1, separated by the dichroic mirror 11, and transmitted by the sampling end flat lens 9-2 to emit ultraviolet light with a wavelength of 365nm, and irradiates the fluorescent substance in the sample cup 17.

[0026] The fluorescent substance in the sample cup 17 absorbs ultraviolet light and emits strong light that is transmitted by the sampling end flat lens 9-2, separated by the dichroic mirror 11, filtered by the detection filter 10-2, and transmitted by the detection end flat lens 9-3 before being received by the light receiving end of the fluorescence detection sensor 12.

[0027] The fluorescent detection head part is connected to the detection head body 7 and the sliding frame 13 through the longitudinal transmission part to form a longitudinal controllable movement connection, and the sliding frame 13 and the guide rail 14 are connected to the inspection instrument base 1 through the transverse transmission part to form a transverse controllable movement connection.

[0028] When in use, under the control of the control system, the longitudinal movement between the detection head body 7 and the sliding frame 13 and the lateral movement between the sliding frame 13 and the guide rail 14 enable the detection head body 7 to detect the sample cups 17 on the constant temperature sample detection bracket 4 one by one.

[0029] The upper cover 15 of the inspection instrument is provided with operation doors 16 corresponding to the sample cup receiving hole 5 and the reagent incubation cup receiving hole 6 respectively.

[0030] When in use, the operation door 16 is opened to implement the exchange operation of the sample cup receiving hole 5 and the sample cup 17 on the reagent incubation cup receiving hole 6.

[0031] The sample cup 17 has a flat bottom and a conical, highly transparent cup body.

[0032] The sample cup 17 with a flat bottom makes it easier for the fluorescent material to be evenly distributed on the flat bottom.

[0033] The sample cup 17 with a conical cup body facilitates the accumulation of fluorescent substances at the bottom of the cup body.

[0034] In the embodiment of the present invention, the constant temperature sample incubation support 2 and the constant temperature sample detection support 4 are respectively provided with heating plates 18 to keep the sample cup 17 in a constant temperature state.

[0035] In the embodiment of the present invention, two sets of light source optical paths and detection optical paths are arranged on the detection head body 7 of the fluorescence detection head part. The two sets of light source optical paths and detection optical paths can respectively emit light of different wavelengths (such as ultraviolet light of 365nm wavelength or blue light of 470nm wavelength), and the two sets of optical paths sequentially perform two tests on the same sample cup 17, which can expand the detection range or mutually verify the detection of fluorescent substances.

Claims

1. A liquid phase fluorescence detector, comprising: The inspection instrument base, the inspection instrument upper cover, the fluorescence detection head part, the detection head movement control part and the sample cup are characterized by: The inspection instrument base (1) is provided with a constant temperature sample incubation support (2), a constant temperature detection agent incubation support (3) and a constant temperature sample detection support (4); the constant temperature sample incubation support (2) and the constant temperature sample detection support (4) are respectively provided with a group of sample cup receiving holes (5) for being detected, and the constant temperature detection agent incubation support (3) is provided with a group of sample cup receiving holes (6) for reagent incubation; The fluorescence detection head part comprises: a detection head body (7), an LED detection light source (8), a light source flat lens (9-1), a sampling end flat lens (9-2), a detection end flat lens (9-3), a light source filter (10-1), a detection filter (10-2), a dichroic mirror (11) and a fluorescence detection sensor (12); On the detection head body (7), a light source optical path is formed by an LED detection light source (8), a light source flat lens (9-1), a light source filter (10-1), a dichroic mirror (11) and a sampling end flat lens (9-2); a detection optical path is formed by the sampling end flat lens (9-2), the dichroic mirror (11), the detection filter (10-2) and the detection end flat lens (9-3); and a light receiving end of the fluorescence detection sensor (12) is connected to the detection end flat lens (9-3) of the detection optical path; The fluorescent detection head part is connected to the detection head body (7) and the sliding frame (13) through the longitudinal transmission part to form a longitudinal controllable movement connection, and the sliding frame (13) and the guide rail (14) are connected to the inspection instrument base (1) through the transverse transmission part to form a transverse controllable movement connection; The upper cover (15) of the inspection instrument is provided with operation doors (16) corresponding to the sample cup receiving hole (5) and the reagent incubation cup receiving hole (6). The sample cup (17) has a flat bottom and a conical light-transmitting cup body.

2. A liquid phase fluorescence detector according to claim 1, characterized in that: The constant temperature sample incubation support (2) and the constant temperature sample detection support (4) are provided with heating plates (18) respectively.

3. A liquid phase fluorescence detector according to claim 1 or 2, characterized in that: On the detection head body (7) of the fluorescent detection head part, two sets of light source optical paths and detection optical paths are arranged.

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