Sample adding control method and sample adding system

Abstract

The invention relates to a sample adding control method and a sample adding system. The sample adding control method comprises the following steps: cleaning a sample adding needle; the injection unit controls the sample adding needle to suck a first preset amount of gas at a first preset speed, and establishes a first gas column in the sample adding needle; the injection unit controls the sample adding needle to suck the sample adding liquid, and after liquid suction is completed, the injection unit controls the sample adding needle to discharge the sample adding liquid to the reaction container at a second preset speed. The first gas column can isolate cleaning liquid from sample adding liquid, so that the cleaning liquid and the sample adding liquid are prevented from being mutually contacted and mixed. Moreover, the injection unit can control the sample adding needle to suck a first preset amount of gas at a first preset speed, so that a stable and reliable first gas column is established in the sample adding needle, the amount of residual liquid on the inner wall of the sample adding needle after the first gas column is established is reduced as much as possible, the sample adding liquid is prevented from being mixed with the residual liquid, and the accuracy during trace sample adding is improved; and the accuracy of a measurement result is improved.

Description

Technical Field

[0001] The present application relates to the field of medical device technology, and in particular to a sample addition control method and a sample addition system. Background Art

[0002] Automatic analyzers analyze body fluids, measure multiple biochemical indicators, and conduct comprehensive analysis of the measured results, which is helpful for the diagnosis, prognosis and functional evaluation of diseases. Usually, automatic analyzers use a sample addition system to add samples and reagents to the reaction container, and the accuracy of the sample addition system will have the most direct impact on the measurement results. Therefore, the accuracy of the sample addition system is required to be high when transferring trace liquids.

[0003] When transferring samples and reagents after cleaning, the current sample injection system will have residual cleaning fluid on the inner wall of the injection needle. The cleaning fluid will mix with the sample or reagent sucked up by the injection needle, making it impossible to guarantee the accuracy of micro-amount injection, thus affecting the accuracy of the measurement results. Summary of the invention

[0004] Based on this, it is necessary to provide a sampling control method and a sampling system to address the problem that the residual liquid on the inner wall of the sampling needle mixes with the reagent or sample and affects the sampling accuracy. The method can realize the micro-sampling operation of the sampling liquid and can avoid the mixing of the sampling liquid and the residual liquid on the inner wall of the sampling needle as much as possible to ensure the accuracy of micro-sampling, thereby improving the accuracy of the measurement results.

[0005] A sample addition control method, comprising:

[0006] Clean the sample needle;

[0007] The injection unit controls the sample adding needle to absorb a first preset amount of gas at a first preset speed and establishes a first gas column in the sample adding needle;

[0008] The injection unit controls the sample adding needle to absorb the sample adding liquid. After the sample adding liquid is absorbed, the injection unit controls the sample adding needle to discharge the sample adding liquid into the reaction container at a second preset speed.

[0009] In one embodiment of the present application, the step of cleaning the sample injection needle at least includes:

[0010] Controlling the sample adding needle to move to the cleaning tank to clean the sample adding needle;

[0011] When cleaning the inner wall of the sample injection needle, controlling the injection unit to establish a preset amount of cleaning liquid;

[0012] When the inner wall cleaning valve is closed, the injection unit controls the sample addition needle to discharge the preset amount of cleaning liquid.

[0013] In one embodiment of the present application, the step of cleaning the sample injection needle at least further comprises:

[0014] When the sample adding needle discharges the preset liquid volume, the cleaning pool is controlled to maintain negative pressure to pump out the waste liquid, so that the cleaning liquid in the sample adding needle is flush with the needle tip of the sample adding needle.

[0015] In one embodiment of the present application, the sample addition control method includes at least one of the following items:

[0016] Item 1, the first preset speed ranges from 60 μL / s to 80 μL / s;

[0017] Item 2, the range of the second preset speed is 80 μL / s to 160 μL / s;

[0018] The third item, the second preset speed is greater than the first preset speed;

[0019] Item 4: the first preset amount is greater than the volume of the tip of the sample adding needle + the volume of the reduced diameter section of the sample adding needle × 2;

[0020] Fifthly, the sample adding needle absorbs the sample adding liquid at a third preset speed, and the range of the third preset speed is 40 μL / s to 80 μL / s.

[0021] In one embodiment of the present application, the first preset speed and the second preset speed are obtained by the following formula:

[0022]

[0023] Wherein, V is the linear flow velocity of the liquid in the sample needle, d is the inner diameter of the sample needle, l is the length of the sample pipe between the injection unit and the sample needle, R is the pipe resistance coefficient of the sample pipe, and Re is the Reynolds number of the liquid in the sample needle.

[0024] In one embodiment of the present application, the sample addition control method further includes:

[0025] When the Reynolds number of the liquid in the sample injection needle is less than 2000, calculating the linear flow rate of the liquid;

[0026] converting the linear flow rate into a volumetric flow rate;

[0027] Selecting multiple groups of data smaller than the volume flow rate as experimental flow rates, and selecting multiple groups of inhaled amounts of the first gas column as experimental gas amounts;

[0028] A repeatability experiment is performed on each of the experimental flow rates and each of the experimental gas volumes to determine the first preset speed and the first preset volume.

[0029] In one embodiment of the present application, the sample addition control method further includes:

[0030] The sample addition needle independently transfers each sample addition liquid into the reaction container;

[0031] After transferring the previous sample liquid and before aspirating the next sample liquid, the sample needle is cleaned, and the first gas column is established after the sample needle is cleaned;

[0032] or,

[0033] The sample addition control method further comprises:

[0034] After the sample adding needle is controlled to absorb a sample adding liquid, the outer wall of the sample adding needle is cleaned;

[0035] The injection unit controls the sample adding needle to absorb a second preset amount of gas and establishes a second gas column in the sample adding needle;

[0036] Controlling the sample adding needle to absorb another sample adding liquid;

[0037] After the liquid aspiration is completed, the injection unit controls the sample addition needle to discharge each sample addition liquid in the sample addition needle into the reaction container.

[0038] In one embodiment of the present application, the amount of sample liquid discharged from the injection unit into the reaction container is the sum of the aspirated amount and the predetermined amount;

[0039] The aspirated amount is the amount of the sample liquid aspirated by the sample needle, or the aspirated amount is the sum of the amount of the sample liquid aspirated by the sample needle and a second preset amount of the second gas column, and the second preset amount is smaller than the first preset amount.

[0040] A sample loading system, applied to the sample loading control method as described in any one of the above technical features, the sample loading system comprises an injection component, an inner wall cleaning component, an outer wall cleaning component and a cleaning pump;

[0041] The injection assembly comprises an injection unit, a sample addition needle and a sample addition pipeline, wherein the sample addition pipeline connects the injection unit and the sample addition needle;

[0042] The inner wall cleaning assembly is used to clean the inner wall of the injection needle, and comprises an inner wall cleaning pipeline and an inner wall cleaning valve, wherein the inner wall cleaning pipeline is connected with the cleaning pump and the injection unit, and the inner wall cleaning valve is arranged on the inner wall cleaning pipeline to control the on-off of the inner wall cleaning pipeline;

[0043] The outer wall cleaning component includes an outer wall cleaning pipeline, a cleaning tank, a negative pressure pump, a discharge pipeline and an outer wall cleaning valve. The outer wall cleaning pipeline connects the cleaning pump and the cleaning tank, the discharge pipeline connects the cleaning tank, the outer wall cleaning valve is arranged on the outer wall cleaning pipeline to control the opening and closing of the outer wall cleaning pipeline, and the negative pressure pump is arranged on the discharge pipeline to draw negative pressure into the cleaning tank.

[0044] In one embodiment of the present application, the cleaning pool includes a cleaning body and a plurality of cleaning holes arranged in the cleaning body, the cleaning body is connected to the outer wall cleaning pipeline and the drainage pipeline, and the plurality of cleaning holes are evenly arranged around the central axis of the cleaning body;

[0045] The cleaning hole is arranged obliquely relative to the central axis of the cleaning body and is aligned with the central axis of the cleaning body.

[0046] After adopting the above technical solution, this application has at least the following technical effects:

[0047] The sample addition control method and sample addition system of the present application, after cleaning the sample addition needle, the injection unit can control the sample addition needle to absorb gas to establish a first gas column in the sample addition needle, and the first gas column can isolate the cleaning liquid and the sample addition liquid to avoid contact and mixing between the cleaning liquid and the sample addition liquid. Moreover, when the injection unit establishes the first gas column in the sample addition needle, the injection unit can control the sample addition needle to absorb a first preset amount of gas at a first preset speed to establish a stable and reliable first gas column in the sample addition needle, and minimize the amount of residual liquid on the inner wall of the sample addition needle after the first gas column is established, thereby avoiding mixing of the sample addition liquid and the residual liquid, improving the accuracy of micro-sampling, and thereby improving the accuracy of the measurement results. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] FIG1 is a schematic structural diagram of a sample loading system according to an embodiment of the present application;

[0049] FIG2 is a flow chart of a sample addition control method of the sample addition system shown in FIG1 ;

[0050] FIG3 is a cleaning flow chart of the sample injection needle shown in FIG1 ;

[0051] FIG4 is a schematic diagram of the control of cleaning the sample addition needle of the sample addition system shown in FIG1 ;

[0052] FIG5 is a schematic diagram of the structure of a sample addition needle in the sample addition system shown in FIG1 ;

[0053] FIG6 is a flow chart of determining a first preset speed in the sample addition control method shown in FIG2 ;

[0054] FIG7 is a sample loading flow chart of an embodiment of a sample loading needle shown in FIG1 ;

[0055] FIG8 is a sample loading flow chart of another embodiment of the sample loading needle shown in FIG1 ;

[0056] FIG. 9 is a schematic diagram showing the distribution of the sample addition liquids and gas columns in the sample addition needle after the sample addition is performed using the method shown in FIG. 8 .

[0057] Wherein: 100, sample adding system; 110, injection assembly; 111, injection unit; 112, sample adding needle; 1121, needle tube; 1122, reducing section; 1123, needle tip; 113, sample adding pipeline; 120, inner wall cleaning assembly; 121, inner wall cleaning pipeline; 122, inner wall cleaning valve; 130, outer wall cleaning assembly; 131, outer wall cleaning pipeline; 132, outer wall cleaning valve; 133, cleaning tank; 134, negative pressure pump; 135, discharge pipeline; 140, cleaning pump; 200, first gas column; 300, second gas column; 400, reaction vessel. DETAILED DESCRIPTION

[0058] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0059] In the description of the present application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0060] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, if the term "plurality" appears, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0061] In this application, unless otherwise clearly specified and limited, if the terms "installed", "connected", "connected", "fixed" and the like appear, these terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0062] In the present application, unless otherwise clearly specified and limited, if there is a description that a first feature is "on" or "below" a second feature, etc., or the like, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0063] It should be noted that if an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. If an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are for illustrative purposes only and do not represent the only implementation method.

[0064] Usually, the automatic analyzer uses a sample loading system to add samples and reagents to the reaction container. The accuracy of the sample loading system will have the most direct impact on the measurement results, so the accuracy of the sample loading system is required to be high when transferring trace liquids. When the current sample loading system transfers samples and reagents after cleaning, cleaning liquid will remain on the inner wall of the sample loading needle. The cleaning liquid will mix with the sample or reagent sucked by the sample loading needle, and the accuracy of the micro-amount of sample loading cannot be guaranteed, which affects the accuracy of the measurement results.

[0065] Referring to Figures 1 and 2, for this purpose, the present application provides a sampling control method. Figure 1 is a structural schematic diagram of a sampling system 100 of an embodiment of the present application, and Figure 2 is a flow chart of a sampling control method of the sampling system 100 shown in Figure 1. The sampling control method is applied to the sampling system 100, and the sampling system 100 can transfer the sampling liquid to the reaction container 400. The sampling liquid here includes samples and reagents. The sampling system 100 can transfer the sample and the reagent to the reaction container 400, and the automatic analyzer processes the sample to measure the sample, thereby obtaining relevant parameters of the sample. Among them, the automatic analyzer is, for example, an immunoassay analyzer (such as an electrochemiluminescence analyzer) or a biochemical analyzer, etc. It should be noted that the sampling liquid is not limited to samples and reagents, but can also be any one of the samples and reagents.

[0066] The sample addition control method can realize the micro-sample addition operation of the sample addition liquid, and can avoid the sample addition liquid from mixing with the residual liquid on the inner wall of the sample addition needle 112 as much as possible, so as to ensure the accuracy of the micro-sample addition, thereby improving the accuracy of the measurement result. To better explain the sample addition control method, the specific structure of the sample addition system 100 is briefly described here.

[0067] In one embodiment, the sample adding system 100 includes an injection assembly 110, an inner wall cleaning assembly 120, an outer wall cleaning assembly 130 and a cleaning pump 140. The injection assembly 110 includes an injection unit 111, a sample adding needle 112 and a sample adding pipeline 113, and the sample adding pipeline 113 connects the injection unit 111 and the sample adding needle 112. The inner wall cleaning assembly 120 is used to clean the inner wall of the sample adding needle 112, and includes an inner wall cleaning pipeline 121 and an inner wall cleaning valve 122. The inner wall cleaning pipeline 121 connects the cleaning pump 140 and the injection unit 111. The inner wall cleaning valve 122 is arranged on the inner wall cleaning pipeline 121 to control the on and off of the inner wall cleaning pipeline 121. The outer wall cleaning component 130 includes an outer wall cleaning pipeline 131, an outer wall cleaning valve 132, a cleaning pool 133, a negative pressure pump 134 and a discharge pipeline 135. The outer wall cleaning pipeline 131 connects the cleaning pump 140 and the cleaning pool 133, the discharge pipeline 135 connects the cleaning pool 133, the outer wall cleaning valve 132 is arranged on the outer wall cleaning pipeline 131 to control the opening and closing of the outer wall cleaning pipeline 131, and the negative pressure pump 134 is arranged on the discharge pipeline 135 to draw negative pressure into the cleaning pool 133.

[0068] The injection assembly 110 is a component in the sample loading system 100 that performs the suction and discharge actions. The injection assembly 110 can absorb and discharge liquid, that is, the injection assembly 110 can absorb the sample loading liquid or discharge the sample loading liquid. The injection assembly 110 can also absorb and discharge gas. It is worth noting that no matter whether the sample loading liquid is a sample or a reagent, the actions performed by the injection assembly 110 are the same. The following text only describes in detail the process of transferring samples and reagents by the sample loading needle 112 in the specific embodiment of the transfer by the sample loading needle 112. In other embodiments, only the sample loading liquid replaces the sample or reagent. It should be noted that the gas can be, for example, air, or other gases that do not chemically react with the sample loading liquid.

[0069] The inner wall cleaning component 120 is used to clean the inner wall of the injection component 110, and the outer wall cleaning component 130 can clean the outer wall of the injection component 110. Before adding samples and after transferring the sample liquid, the injection component 110 needs to clean the outer wall and inner wall of the injection component 110 to clean the residual liquid on the inner wall and outer wall of the injection component 110, so as to avoid the sample liquid absorbed by the injection component 110 from contacting the residual liquid on the inner wall and outer wall of the injection component 110, thereby avoiding contamination of the sample liquid.

[0070] Specifically, the injection assembly 110 includes an injection unit 111, a sample needle 112, and a sample pipette 113. One end of the sample pipette 113 is connected to the injection unit 111, and the other end of the sample pipette 113 is connected to the sample needle 112, that is, the injection unit 111 is connected to the sample needle 112 through the sample pipette 113. The injection unit 111 can perform precise suction and discharge actions to control the sample needle 112 to perform suction and discharge actions. During suction, the injection unit 111 can suck the sample liquid or gas into the sample needle 112, and during discharge, the injection unit 111 can control the sample needle 112 to discharge the sample liquid or gas.

[0071] The injection unit 111 can reciprocate to control the sample needle 112 to perform suction and discharge actions and return to the initial position. When the injection unit 111 is in the initial position, the sample needle 112 does not perform suction action or completely discharge the sample liquid. After the injection unit 111 performs one suction and discharge action, the injection unit 111 returns to the initial position.

[0072] Optionally, the injection unit 111 is a precision plunger pump or a syringe. Optionally, the injection assembly 110 further includes a driving member, which is disposed at the end of the injection unit 111, and the driving member controls the injection unit 111 to reciprocate, thereby controlling the sample injection needle 112 to perform the suction and discharge actions. Optionally, the driving member is electrically connected to the controller of the automatic analyzer to realize automatic control of the injection assembly 110.

[0073] In the inner wall cleaning assembly 120, one end of the inner wall cleaning pipeline 121 is connected to the cleaning pump 140, and the other end of the inner wall cleaning pipeline 121 is connected to the injection unit 111. When the cleaning pump 140 is turned on, the cleaning pump 140 can transport the cleaning liquid to the injection unit 111 through the inner wall cleaning pipeline 121, and then transport it to the sample injection needle 112 through the injection unit 111 and the sample injection pipeline 113 to clean the inner wall of the sample injection needle 112, thereby reducing the sample injection liquid remaining on the inner wall of the sample injection needle 112 as much as possible, thereby avoiding affecting the next operation of the sample injection needle 112 to absorb the sample injection liquid.

[0074] The inner wall cleaning valve 122 is arranged on the inner wall cleaning pipeline 121 to control the on-off of the inner wall cleaning pipeline 121. When the inner wall cleaning valve 122 is opened, the inner wall cleaning pipeline 121 is a passage, and the cleaning pump 140 can deliver the cleaning liquid to the injection unit 111; when the inner wall cleaning valve 122 is closed, the inner wall cleaning pipeline 121 is an open circuit, and the cleaning pump 140 cannot deliver the cleaning liquid to the injection unit 111.

[0075] In this embodiment, the cleaning liquid is pure water. Of course, in other embodiments of the present application, the cleaning liquid can also be other liquids containing cleaning agents or other liquids that can achieve a cleaning effect.

[0076] One end of the outer wall cleaning component 130 is connected to the cleaning pump 140, and the other end extends to the waste water tank. The sample adding needle 112 moves to the outer wall cleaning component 130, and the waste liquid when cleaning the inner wall of the sample adding needle 112 can be discharged into the outer wall cleaning component 130. The outer wall of the sample adding needle 112 is cleaned by the outer wall cleaning component 130, and the cleaning liquid on the inner wall of the sample adding needle 112 is discharged at the same time.

[0077] In the outer wall cleaning assembly 130, one end of the outer wall cleaning pipeline 131 is connected to the cleaning pump 140, the other end of the outer wall cleaning pipeline 131 is connected to the cleaning tank 133, one end of the discharge pipeline 135 is connected to the cleaning tank 133, and the other end is connected to the waste water tank. When cleaning the outer wall of the sample adding needle 112, the sample adding needle 112 moves into the cleaning tank 133 and extends into the cleaning tank 133. When the cleaning pump 140 is turned on, the cleaning pump 140 can transport the cleaning liquid to the cleaning tank 133 through the outer wall cleaning pipeline 131, and at this time, the cleaning liquid can clean the outer wall of the sample adding needle 112.

[0078] The negative pressure pump 134 is arranged on the liquid discharge pipeline 135. When cleaning, the negative pressure pump 134 is turned on. The negative pressure pump 134 can pump negative pressure into the cleaning pool 133 and discharge the waste liquid through the liquid discharge pipeline 135. The negative pressure pump 134 uses negative pressure to pump and discharge the waste liquid in the cleaning pool 133 to prevent the waste liquid from remaining on the outer wall of the sample addition needle 112. Moreover, the waste liquid from the inner wall of the sample addition needle 112 is also discharged into the cleaning pool 133 and the waste liquid is discharged by the negative pressure pump 134.

[0079] The outer wall cleaning valve 132 is arranged on the outer wall cleaning pipeline 131 to control the on-off of the outer wall cleaning pipeline 131. When the outer wall cleaning valve 132 is opened, the outer wall cleaning pipeline 131 is a passage, and the cleaning pump 140 can deliver the cleaning liquid to the cleaning tank 133. When the outer wall cleaning valve 132 is closed, the outer wall cleaning pipeline 131 is an open circuit, and the cleaning pump 140 cannot deliver the cleaning liquid to the cleaning tank 133. Optionally, both the inner wall cleaning valve 122 and the outer wall cleaning valve 132 are solenoid valves, or other valves capable of realizing on-off control.

[0080] When cleaning the sample needle 112, the sample needle 112 is moved to the cleaning pool 133, and only the inner wall of the sample needle 112 can be cleaned, only the outer wall of the sample needle 112 can be cleaned, or both the inner wall and the outer wall of the sample needle 112 can be cleaned. Moreover, the cleaning of the inner wall and the cleaning of the outer wall of the sample needle 112 can be performed simultaneously or sequentially, depending on actual needs.

[0081] The specific implementable form of the sample loading control method of one embodiment is described below in conjunction with the specific structure of the sample loading system 100 .

[0082] Referring to FIG. 1 and FIG. 3 , FIG. 3 is a cleaning flow chart of the sample addition needle 112 shown in FIG. 1 . In one embodiment, the sample addition control method includes:

[0083] S100, cleaning the sample adding needle 112.

[0084] Before starting the first sample addition operation or the next sample addition operation, the sample addition needle 112 is cleaned by the inner wall cleaning component 120 and the outer wall cleaning component 130 to remove as much residual liquid as possible from the outer wall and the inner wall of the sample addition needle 112. In this way, when the sample addition needle 112 absorbs the sample addition liquid, it can prevent the residual liquid on the outer wall of the sample addition needle 112 from contaminating the sample addition liquid in the container, and it can also prevent the residual liquid on the inner wall of the sample addition needle 112 from contaminating the sample addition liquid absorbed by the sample addition needle 112, thereby improving the accuracy of the sample measurement result.

[0085] S200 , the injection unit 111 controls the sample adding needle 112 to absorb a first preset amount of gas at a first preset speed, and establishes a first gas column 200 in the sample adding needle 112 .

[0086] After the sample needle 112 is cleaned, there will be cleaning liquid in the sample needle 112. At this time, the injection unit 111 controls the sample needle 112 to absorb gas to establish a first gas column 200 (as shown in FIG. 9 ) in the sample needle 112. In this way, when the sample needle 112 absorbs the sample liquid again, the first gas column 200 can play an isolating role in the sample needle 112 to separate the cleaning liquid from the sample liquid, thereby preventing the cleaning liquid from mixing with the sample liquid, and further preventing the cleaning liquid from contaminating the sample liquid.

[0087] Moreover, when the injection unit 111 controls the sample adding needle 112 to absorb gas, it absorbs a first preset amount of gas at a first preset speed, so that a stable and reliable first gas column 200 can be established in the sample adding needle 112. The first gas column 200 of the first preset amount can effectively separate the cleaning liquid and the sample adding liquid, and can also reduce the residual liquid (residual cleaning liquid) on the inner wall of the sample adding needle 112 as much as possible.

[0088] When the sampling needle 112 absorbs a first preset amount of gas at a first preset speed, the gas can flow stably in the sampling needle 112, thereby improving the stability and reliability of gas absorption. At the same time, when the gas flows, it can also take away as much residual liquid as possible on the inner wall of the sampling needle 112 to avoid contact between the residual liquid on the inner wall of the sampling needle 112 and the sample liquid, thereby avoiding contamination, and ensuring the amount of sample liquid absorbed in the sampling needle 112, thereby improving the accuracy of micro-sampling.

[0089] It should be noted that the gas used to establish the gas column may be, for example, air, or other gases that do not chemically react with the sample liquid, which will not be described in detail below.

[0090] S300, the injection unit 111 controls the sample needle 112 to absorb the sample liquid. After the sample liquid is absorbed, the injection unit 111 controls the sample needle 112 to discharge the sample liquid into the reaction container 400 at a second preset speed.

[0091] After the first gas column 200 is established in the sample needle 112, the sample needle 112 is controlled to move to the sample position, and the injection unit 111 controls the sample needle 112 to absorb the sample liquid at the sample position. After the sample needle 112 completes the liquid absorption, the sample needle 112 is controlled to move from the sample position to the liquid discharge position, and the reaction container 400 is placed in the liquid discharge position. The sample needle 112 is controlled to move above the reaction container 400 at the liquid discharge position, and then the injection unit 111 controls the sample needle 112 to discharge the sample liquid into the reaction container 400 at the second preset speed to complete the sample addition operation.

[0092] After completing a sample addition operation, the automatic analyzer performs incubation and other treatments on the reaction container 400 to obtain relevant parameters of the sample. It is worth noting that the movement of the sample addition needle 112 in the present application is achieved by the driving mechanism of the sample addition needle 112, and the driving mechanism of the sample addition needle 112 is a conventional component in the automatic analyzer and is not described in detail here. Optionally, the reaction container 400 includes but is not limited to a reaction cup, and can also be a reaction slide, etc.

[0093] Moreover, when the sample needle 112 discharges the sample liquid into the reaction container 400 at the second preset speed, the sample liquid can be discharged under laminar flow, and at the same time, the dynamic stability of the sample liquid during discharge is ensured, splashing of the sample liquid is avoided, and the first gas column 200 can effectively play an isolation role.

[0094] When the sample adding system 100 of the present application is used for sample adding operation, the sample adding needle 112 is first controlled to move into the cleaning pool 133, and the inner wall cleaning assembly 120 and the outer wall cleaning assembly 130 are controlled to clean the outer wall and the inner wall of the sample adding needle 112 respectively. After the cleaning is completed, the sample adding needle 112 is removed from the cleaning pool 133. Then, the injection unit 111 controls the sample adding needle 112 to absorb a first preset amount of gas at a first preset speed to establish a first gas column 200 at the needle tip 1123 of the sample adding needle 112.

[0095] Subsequently, the sample needle 112 is controlled to move to the sample adding position, and the injection unit 111 controls the sample needle 112 to absorb the sample adding liquid. At this time, the first gas column 200 can isolate the cleaning liquid and the sample adding liquid to prevent the two from mixing. After the liquid aspiration is completed, the sample needle 112 is controlled to move to the top of the corresponding reaction container 400 in the liquid discharge position, and the injection unit 111 controls the sample needle 112 to discharge the sample adding liquid into the reaction container 400 at a second preset speed.

[0096] After the sample addition is completed, the sample addition needle 112 is controlled to repeat the steps S100 to S300 until all sample addition operations are completed. Meanwhile, the automatic analyzer performs an incubation operation on the sample in the reaction container 400. In this way, the operation efficiency of the instrument can be improved.

[0097] In the sample addition control method of the above embodiment, after cleaning the sample addition needle 112, the injection unit 111 can control the sample addition needle 112 to absorb gas to establish a first gas column 200 in the sample addition needle 112. The first gas column 200 can isolate the cleaning liquid from the sample addition liquid to prevent the cleaning liquid and the sample addition liquid from contacting and mixing with each other. Moreover, when the injection unit 111 establishes the first gas column 200 in the sample addition needle 112, the injection unit 111 can control the sample addition needle 112 to absorb a first preset amount of gas at a first preset speed to establish a stable and reliable first gas column 200 in the sample addition needle 112, and minimize the amount of residual liquid on the inner wall of the sample addition needle 112 after the first gas column 200 is established, thereby preventing the sample addition liquid from mixing with the residual liquid, improving the accuracy of micro-sampling, and thereby improving the accuracy of the measurement results.

[0098] Referring to FIG. 2 , in one embodiment, the step of cleaning the sample injection needle 112 at least includes:

[0099] S110 , controlling the sample adding needle 112 to move to the cleaning pool 133 to clean the sample adding needle 112 .

[0100] Before starting the first sample addition operation or the next sample addition operation, the sample addition needle 112 is cleaned by controlling the sample addition needle 112 to move into the cleaning tank 133, opening the cleaning pump 140, the inner wall cleaning valve 122 and the outer wall cleaning valve 132, and cleaning the inner wall and the outer wall of the sample addition needle 112 to clean the residual liquid on the inner wall and the outer wall of the sample addition needle 112. The cleaning waste liquid is collected and discharged through the cleaning tank 133.

[0101] It is worth noting that here, the inner wall and the outer wall of the sample adding needle 112 are cleaned at the same time, so the cleaning pump 140, the inner wall cleaning valve 122 and the outer wall cleaning valve 132 are opened at the same time. When only the outer wall is cleaned, only the cleaning pump 140 and the outer wall cleaning valve 132 can be opened, and when only the inner wall of the sample adding needle 112 is cleaned, only the cleaning pump 140 and the inner wall cleaning valve 122 are opened, which will not be repeated in the following text.

[0102] S120 , when cleaning the inner wall of the sample adding needle 112 , the injection unit 111 is controlled to create a preset amount of cleaning liquid.

[0103] While cleaning the inner wall, the injection unit 111 performs a reset operation, and then the injection unit 111 controls the sample addition needle 112 to perform a suction action to provide a pumping volume for subsequent actions of the injection unit 111 .

[0104] S130, when the inner wall cleaning valve 122 is closed, the injection unit 111 controls the sample adding needle 112 to discharge the preset amount of cleaning liquid.

[0105] After the sample adding needle 112 absorbs the preset amount of cleaning liquid, the cleaning pump 140 and the inner wall cleaning valve 122 are controlled to be closed, and then the injection unit 111 controls the sample adding needle 112 to discharge the preset amount of cleaning liquid.

[0106] It is understandable that when the inner wall cleaning valve 122 is closed, the inner wall cleaning valve 122 will generate a certain suction force, which will naturally form an air column at the needle tip 1123 of the sample adding needle 112. If the first air column 200 is directly established on the basis of the air column or the sample adding liquid is sucked, the stability of the first air column 200 will be affected and the sample adding liquid will be mixed with the inner wall of the sample adding needle 112, affecting the accuracy of the sample adding.

[0107] Therefore, in the sample addition control method of the present application, when cleaning the inner wall of the sample addition needle 112, the injection unit 111 first controls the sample addition needle 112 to absorb a preset amount of cleaning liquid, and after closing the inner wall cleaning valve 122, the injection unit 111 controls the sample addition needle 112 to discharge the preset amount of liquid. At this time, the air column naturally formed at the needle tip 1123 of the sample addition needle 112 will also be discharged together with the cleaning liquid.

[0108] Moreover, the injection unit 111 controls the preset liquid volume discharged by the sample needle 112 = the gas volume of the gas column naturally formed at the needle tip 1123 of the sample needle 112 + the volume of part of the cleaning liquid. In other words, the preset liquid volume is generally greater than the gas volume of the gas column naturally formed. When the injection unit 111 controls the sample needle 112 to discharge the preset liquid volume, the gas column at the sample needle 112 and a certain amount of cleaning liquid will be discharged. In this way, there is only cleaning liquid in the sample needle 112, and then a stable and reliable first gas column 200 can be established in the sample needle 112.

[0109] Referring to FIG. 3 , in one embodiment, the step of cleaning the sample injection needle 112 at least further comprises:

[0110] S140 , when the sample adding needle 112 discharges the preset amount of liquid, the cleaning pool 133 is controlled to maintain negative pressure to pump out the waste liquid, so that the cleaning liquid in the sample adding needle 112 is flush with the needle tip 1123 of the sample adding needle 112 .

[0111] When cleaning the sample needle 112, the negative pressure pump 134 is controlled to work, and the negative pressure pump 134 can continuously discharge the waste liquid in the cleaning tank 133. When the sample needle 112 discharges a preset amount of liquid, the cleaning tank 133 is controlled to maintain a negative pressure state, so that the air column naturally formed at the needle tip 1123 of the sample needle 112 will also be discharged together with the cleaning liquid.

[0112] In addition, when the cleaning liquid is discharged, the cleaning liquid may form droplets at the needle tip 1123 of the sample needle 112, and the droplets can be drawn away by the negative pressure of the negative pressure pump 134, ensuring that the cleaning liquid is flush with the needle tip 1123 of the sample needle 112, that is, after the sample needle 112 is cleaned, there is no gas in the sample needle 112, which is convenient for establishing the first gas column 200 in the sample needle 112 later.

[0113] S150 , controlling the sample adding needle 112 to be removed from the cleaning pool 133 .

[0114] The injection unit 111 controls the sample needle 112 to discharge a preset amount of liquid, and after ensuring that the cleaning liquid in the sample needle 112 is flush with the needle tip 1123 of the sample needle 112, the cleaning operation of the sample needle 112 is completed. At this time, the sample needle 112 can be removed from the cleaning pool 133, and then the first gas column 200 can be established in the sample needle 112.

[0115] After the inner wall of the sample needle 112 is cleaned, the injection unit 111 controls the sample needle 112 to discharge a preset amount of liquid while maintaining the negative pressure pump 134 to pump negative pressure into the cleaning pool 133. In this way, it is ensured that the air column generated at the needle tip 1123 of the sample needle 112 when the inner wall cleaning valve 122 is closed in step S120 is discharged, and the cleaning liquid in the sample needle 112 is flush with the needle tip 1123 of the sample needle 112, so that the sample needle 112 is completely filled with the cleaning liquid.

[0116] After the sample needle 112 is cleaned, the sample needle 112 is controlled to be removed from the cleaning pool 133. Then, the injection unit 111 controls the sample needle 112 to absorb a first preset amount of gas at a first preset speed to establish a stable and reliable first gas column 200 in the sample needle 112.

[0117] Referring to FIG. 1 to FIG. 4 , FIG. 4 is a control schematic diagram of the sample addition system 100 shown in FIG. 1 for cleaning the sample addition needle 112. Before starting the first sample addition operation or the next sample addition operation, the sample addition needle 112 is cleaned by first controlling the sample addition needle 112 to move into the cleaning tank 133, opening the cleaning pump 140, the inner wall cleaning valve 122, and the outer wall cleaning valve 132, and cleaning the inner wall and the outer wall of the sample addition needle 112 to clean the residual liquid on the inner wall and the outer wall of the sample addition needle 112. The cleaning waste liquid is collected and discharged through the cleaning tank 133.

[0118] While cleaning the inner wall, the injection unit 111 performs a reset operation, and then the injection unit 111 controls the sample needle 112 to perform an aspirating action, at which time the sample needle 112 can aspirate a preset amount of cleaning liquid. After the sample needle 112 aspirates the preset amount of cleaning liquid, the cleaning pump 140 and the inner wall cleaning valve 122 are controlled to close, and then the injection unit 111 controls the sample needle 112 to discharge the preset amount of liquid, and at the same time, the negative pressure pump 134 works, and the negative pressure pump 134 can continuously discharge the waste liquid in the cleaning tank 133.

[0119] While the sample needle 112 discharges the preset amount of liquid, the cleaning pool 133 is controlled to maintain a negative pressure state, so that the air column naturally formed at the needle tip 1123 of the sample needle 112 is also discharged together with the cleaning liquid. In this way, it is ensured that the air column generated at the needle tip 1123 of the sample needle 112 when the inner wall cleaning valve 122 is closed is discharged, and the cleaning liquid in the sample needle 112 is flush with the needle tip 1123 of the sample needle 112, so that the sample needle 112 is completely filled with the cleaning liquid.

[0120] After the sample adding needle 112 is cleaned, the sample adding needle 112 is controlled to be removed from the cleaning pool 133. Subsequently, the injection unit 111 controls the sample adding needle 112 to absorb a first preset amount of gas at a first preset speed to establish a stable and reliable first gas column 200 in the sample adding needle 112. At the same time, the outer wall cleaning valve 132 and the negative pressure pump 134 are controlled to be closed.

[0121] In one embodiment, the preset liquid volume ranges from 15 μL to 30 μL. Exemplarily, the preset liquid volume is 20 μL. When cleaning the inner wall of the sample adding needle 112, the injection unit 111 performs a reset action and then controls the sample adding needle 112 to absorb 20 μL of cleaning liquid. After closing the inner wall cleaning valve 122, the injection unit 111 controls the sample adding needle 112 to discharge 20 μL, where 20 μL = the amount of gas in the gas column + a certain amount of cleaning liquid. Of course, in other embodiments of the present application, the injection unit 111 may also control the sample adding needle 112 to absorb and discharge other preset liquid volumes.

[0122] In one embodiment, the first preset speed ranges from 60 μL / s to 80 μL / s. 60 μL / s to 80 μL / s is a relatively low speed, and when the injection unit 111 controls the sample needle 112 to absorb gas at the first preset speed, the sample needle 112 can absorb gas at a low speed, so as to establish a stable and reliable first gas column 200 in the sample needle 112.

[0123] Exemplarily, the range of the first preset speed is 80 μL / s. That is, the injection unit 111 controls the sample adding needle 112 to absorb the first preset amount of gas at a low speed at the first preset speed of 80 μL / s, so as to establish a stable and reliable first gas column 200 in the sample adding needle 112. In this way, the repeatability of the sample adding operation can be improved, and the accuracy of the sample adding can be improved.

[0124] In one embodiment, the second preset speed is in the range of 80 μL / s to 160 μL / s. When the second preset speed is in the range of 80 μL / s to 160 μL / s, the sample liquid in the sample needle 112 can be controlled to be discharged under laminar flow, while ensuring the dynamic stability of the sample liquid discharge and ensuring that the first gas column 200 can play an isolation role.

[0125] Moreover, when the injection unit 111 controls the sample needle 112 to discharge the sample liquid at a second preset speed of 80 μL / s to 160 μL / s, the sample liquid in the sample needle 112 can be discharged as much as possible, reducing the residual liquid on the inner wall of the sample needle 112 to ensure the accuracy of the sample addition.

[0126] In one embodiment, the second preset speed is greater than the first preset speed. That is, the injection unit 111 controls the speed at which the sample needle 112 absorbs gas to be less than the speed at which the sample needle 112 discharges the sample liquid.

[0127] In this way, the first gas column 200 can be stably established to reduce the cleaning liquid remaining on the inner wall of the sample needle 112 and avoid mixing of the sample liquid with the residual liquid. At the same time, it is ensured that the sample liquid in the sample needle 112 can be discharged as much as possible, reducing the sample liquid remaining on the inner wall of the sample needle 112 and ensuring the accuracy of the sample addition.

[0128] Referring to Fig. 5, Fig. 5 is a schematic diagram of the structure of the sample addition needle 112 in the sample addition system 100 shown in Fig. 1. The sample addition needle 112 includes a needle tube 1121, a diameter reducing section 1122 and a needle tip 1123. The diameter reducing section 1122 connects the needle tube 1121 and the needle tip 1123, and one end of the needle tube 1121 away from the diameter reducing section 1122 is connected to the sample addition pipeline 113. The needle tube 1121, the diameter reducing section 1122 and the needle tip 1123 are an integrated structure to ensure the structural strength of the sample addition needle 112.

[0129] In one embodiment, the first preset volume is greater than the volume of the needle tip 1123 of the sample adding needle 112 + the volume of the diameter reducing section 1122 of the sample adding needle 112 × 2. In this way, the first gas column 200 can effectively isolate the cleaning liquid and the sample adding liquid to prevent the cleaning liquid and the sample adding liquid from mixing.

[0130] In one embodiment, the first preset amount is in the range of 25 μL to 30 μL. When the first preset amount is in the range of 25 μL to 30 μL, after the first preset amount of gas establishes the first gas column 200 in the sample injection needle 112, it can effectively isolate the cleaning liquid and the sample injection liquid to prevent the two from mixing.

[0131] Exemplarily, the first preset amount is 30 μL. That is, the injection unit 111 controls the sample adding needle 112 to absorb the first preset amount of gas of 30 μL at a first preset speed of 80 μL / s to establish the first gas column 200 in the sample adding needle 112. Of course, in other embodiments of the present application, the sample adding needle 112 may also absorb other preset amounts of gas to establish the first gas column 200.

[0132] It can be understood that, generally, after the step of cleaning the sample injection needle 112 is completed, the sample injection needle 112 is filled with cleaning liquid. At this time, gas is sucked at a first preset speed to establish a first gas column 200 in the sample injection needle 112. The first preset speed corresponds to the linear flow rate of the cleaning liquid in the sample injection needle 112.

[0133] In one embodiment, the first preset speed and the second preset speed are obtained by a calculation formula:

[0134]

[0135] Wherein, V is the linear flow velocity (m / s) of the liquid in the sample addition needle 112, d is the inner diameter (mm) of the sample addition needle 112, l is the length (m) of the sample addition pipeline 113 between the injection unit 111 and the sample addition needle 112, R is the pipe resistance coefficient of the sample addition pipeline 113, and Re is the Reynolds number of the liquid in the sample addition needle 112.

[0136] The above calculation formula can calculate the first preset speed of the sample needle 112 to establish the first gas column 200, and can also calculate the second preset speed of the sample needle 112 to discharge the sample liquid, and can also calculate the speed of the sample needle 112 to absorb the sample liquid. Here, only the calculation formula for calculating the first preset speed is used as an example for explanation.

[0137] When calculating the first preset speed, the liquid in the sample needle 112 is a cleaning liquid, such as pure water. When the d of the sample needle 112 is 1.5 mm, the Reynolds number Re of the cleaning liquid is less than 2000, and the length l of the sample pipe 113, the density ρ of the cleaning liquid, and the pipe resistance coefficient R of the sample pipe 113 are all known, and the linear flow rate V can be calculated at this time. The first preset speed for the sample needle 112 to establish the first air column 200 is less than the linear speed V, and a suitable speed is selected as the first preset speed to establish a stable and reliable first air column 200 in the sample needle 112. After determining the first preset speed, the sample system 100 absorbs the first air column 200 at the first preset speed.

[0138] Referring to Figures 1, 2 and 6, Figure 6 is a flow chart of determining the first preset speed in the sample addition control method shown in Figure 2. In one embodiment, the sample addition control method can also determine the first preset speed, which further includes:

[0139] S210, when the Reynolds number of the liquid in the sample adding needle 112 is less than 2000, calculating the linear flow rate of the liquid.

[0140] The calculation of the first preset speed using the above calculation formula is used as an example for explanation. When the Reynolds number Re of the cleaning liquid is less than 2000, the flow state of the cleaning liquid is laminar flow, when the Reynolds number Re of the cleaning liquid is greater than 2000 and less than 4000, the flow state of the cleaning liquid transitions from laminar flow to turbulent flow, and when the Reynolds number Re of the cleaning liquid is greater than 4000, the flow state of the cleaning liquid is turbulent flow.

[0141] When the fluid state of the cleaning liquid in the sample injection needle 112 is in turbulence or transitions from laminar flow to turbulence, adjacent liquid layers will mix together, and irregular vortex motion will appear in the flow field, which will affect the stability of the first gas column 200 established in the sample injection needle 112. Therefore, it is necessary to calculate the linear flow rate of the cleaning liquid when the Reynolds number Re of the cleaning liquid is less than 2000.

[0142] Specifically, the actual Reynolds number of the cleaning liquid is first obtained, and the actual Reynolds number is compared with the above Reynolds number range to determine whether the flow state of the cleaning liquid is laminar flow, transitioning from laminar flow to turbulent flow, or turbulent flow. If the flow state of the cleaning liquid is transitioning from laminar flow to turbulent flow or turbulent flow, the sample injection needle 112 is kept stationary for a period of time, and then the actual Reynolds number of the cleaning liquid is obtained, until the flow state of the cleaning liquid is laminar flow, and the linear flow velocity is calculated by the above calculation formula.

[0143] S220, converting the linear flow rate into a volume flow rate.

[0144] It can be understood that linear flow rate refers to the speed at which a fluid passes through a certain cross-section, usually expressed as the distance the fluid passes per unit time, such as m / s; volume flow rate refers to the volume of fluid flowing through a certain cross-section per unit time, usually expressed in liters per minute (L / min), milliliters per minute (mL / min) or microliters per second (μL / s), etc.

[0145] It is worth noting that the method of converting the linear flow rate into the volume flow rate by using the inner diameter of the sample injection needle 112 is a prior art and will not be described in detail here.

[0146] The present application uses the above-mentioned calculation formula to calculate the linear speed of the sample adding needle 112 sucking gas to establish the first gas column 200, and then converts the linear speed into a volume flow rate. The speed of the sample adding needle 112 sucking gas to establish the first gas column 200 is measured by the volume flow rate to ensure the stability and reliability of the first gas column 200 in the sample adding needle 112.

[0147] S230, selecting multiple groups of data that are smaller than the volume flow rate as experimental flow rates, and selecting multiple groups of suction volumes of the first gas column 200 as experimental gas volumes.

[0148] After the linear velocity is converted into a volume flow rate, a sample addition repeatability experiment is required to determine a more appropriate first preset velocity and a first preset amount. Specifically, multiple sets of data are selected as experimental flow rates, each experimental flow rate is less than the volume flow rate and is different from each other; at the same time, multiple suction volumes are selected as the experimental gas volume of the first gas column 200, and each experimental gas volume is different from each other. A more appropriate first preset velocity and a first preset amount are determined based on each experimental flow rate and each experimental gas volume.

[0149] S240, performing a sample addition repeatability experiment on each of the experimental flow rates and each of the experimental gas volumes to determine the first preset speed and the first preset volume.

[0150] Each experimental flow rate and each experimental gas volume can be combined to perform a sample addition repeatability experiment. Specifically, a sample addition repeatability experiment is performed with a combination of an experimental flow rate and an experimental gas volume. At this time, after the sample addition needle 112 is cleaned, a first gas column 200 is established in the sample addition needle 112 according to the experimental flow rate and the experimental gas volume. Subsequently, the sample addition needle 112 absorbs the sample addition liquid and discharges the sample addition liquid into the reaction container 400 at a second preset speed. The average sample addition offset and the coefficient of variation (CV) in the sample addition operation are calculated.

[0151] The experimental flow rate is combined with another experimental gas volume to perform a sample addition repeatability experiment to obtain the average sample addition offset and coefficient of variation CV under the experimental flow rate and the experimental gas volume. This process is repeated to determine the average sample addition offset and coefficient of variation CV corresponding to each experimental flow rate and each experimental gas volume.

[0152] It can be understood that the closer the average sample addition offset is to zero, the higher the accuracy of the sample addition is, and the smaller the coefficient of variation CV is, the better the repeatability of the sample addition operation is. In this way, a suitable experimental speed can be selected as the first preset speed and a suitable experimental gas volume can be selected as the first preset volume according to the experimental results.

[0153] Moreover, in the sample addition repeatability experiment, the amount of sample sucked by the sample addition needle 112 is 5 μL to achieve the transfer of a micro-amount of sample, making it easier to determine the average sample addition offset in the sample addition repeatability experiment to determine the accuracy of the sample addition. Of course, in other embodiments of the present application, in the sample addition repeatability experiment, the sample addition needle 112 can also suck other amounts of sample to perform the sample addition repeatability experiment.

[0154] In this embodiment, the first preset speed and the first preset amount are determined to be more appropriate through the conclusion of the experiment. Under the condition of ensuring the consistency of other timing parameters, the accuracy and repeatability of the sample addition under multiple experimental conditions of transferring 5 μL of sample addition liquid are tested with a high-precision balance. Moreover, the experimental flow rates selected in this embodiment are 40 μL / s, 80 μL / s, and 120 μL / s, and the experimental gas volumes are 10 μL, 20 μL, and 30 μL. The specific experimental results are shown in Table 1.

[0155] Table 1

[0156]

[0157] It can be seen from the experimental data in Table 1 that the first preset speed below 80 μL / s has little effect on the improvement of sample aspiration repeatability and accuracy. Similarly, increasing the length of the first gas column 200 can effectively improve the accuracy of sample aspiration, and increasing the first preset volume to 30 μL can basically meet the industry standard for sample addition to ensure the accuracy of sample addition.

[0158] It is worth noting that in practice, if the time for absorbing the first air column 200 is limited, the longer the amount absorbed by the first air column 200 is, the greater the first preset speed for absorbing the first air column 200 will be, so it is also necessary to make a reasonable selection according to the conditions.

[0159] Referring to FIG. 1 and FIG. 7 , FIG. 7 is a sample addition flow chart of an embodiment of the sample addition needle 112 shown in FIG. 1 . In one embodiment, the sample addition control method further includes:

[0160] S310 , the sample adding needle 112 independently transfers each sample adding liquid into the reaction container 400 .

[0161] That is, the sample needle 112 transfers the sample liquid independently. After the sample needle 112 absorbs a sample liquid at the sample position, the sample needle 112 moves to the top of the reaction container 400 to discharge the sample liquid into the reaction container 400. Subsequently, the sample needle 112 absorbs another sample liquid and transfers the sample liquid to the reaction container 400, and so on and so forth, and each sample liquid is added to the reaction container 400 respectively.

[0162] Specifically, when the sample liquid is a sample and a reagent, after the sample needle 112 absorbs the sample at the sample adding position, the sample needle 112 moves to the top of the reaction container 400 to transfer the sample to the reaction container 400. Similarly, each required reagent is sequentially transferred to the reaction container 400 in this manner.

[0163] S320 , after transferring the previous sample liquid and before aspirating the next sample liquid, the sample needle 112 is cleaned, and the first gas column 200 is established after the sample needle 112 is cleaned.

[0164] That is to say, the sample adding needle 112 needs to be cleaned before the sample adding test, and the sample adding needle 112 also needs to be cleaned after each sample adding liquid is absorbed by the sample adding needle 112. Moreover, after the sample adding needle 112 is cleaned, a stable and reliable first gas column 200 needs to be established in the sample adding needle 112 to reduce the residual liquid on the inner wall of the sample adding needle 112 as much as possible.

[0165] In this embodiment, the sample addition needle 112 transfers the sample and the reagent independently, that is, the sample addition needle 112 absorbs the sample addition liquid once. After the sample addition needle 112 is cleaned and the first gas column 200 is established, the sample addition needle 112 independently absorbs the sample and transfers the sample to the reaction container 400. Then, the sample addition needle 112 is cleaned and the first gas column 200 is established in the above manner, and then the sample addition needle 112 is controlled to absorb the reagent of a single component and add it to the reaction container 400. Similarly, the required sample and reagent are added to the reaction container 400 in this way.

[0166] Referring to FIG. 1 and FIG. 8 , FIG. 8 is a sample addition flow chart of another embodiment of the sample addition needle 112 shown in FIG. 1 . In one embodiment, the sample addition control method further includes:

[0167] S330, after controlling the sample adding needle 112 to absorb a sample adding liquid, the outer wall of the sample adding needle 112 is cleaned.

[0168] After the sample needle 112 absorbs a type of sample liquid at the sample loading position, the sample needle 112 is transferred to the cleaning tank 133, and the outer wall of the sample needle 112 is cleaned by the cooperation of the cleaning pump 140 and the outer wall cleaning component 130 to avoid contamination when the sample needle 112 absorbs another sample liquid.

[0169] S340 , the injection unit 111 controls the sample adding needle 112 to absorb a second preset amount of gas, and establishes a second gas column 300 in the sample adding needle 112 .

[0170] After the outer wall of the sample needle 112 is cleaned, the sample needle 112 is removed from the cleaning pool 133, and then the sample needle 112 is controlled to absorb a second preset amount of gas to establish a second gas column 300 in the sample needle 112. The second gas column 300 can play an isolating role to isolate a sample liquid.

[0171] S350, controlling the sample addition needle 112 to absorb another sample addition liquid.

[0172] After the second gas column 300 is established in the sample addition needle 112, the sample addition needle 112 is controlled to move to the sample addition position, and the injection unit 111 controls the sample addition needle 112 to absorb another type of sample addition liquid. At this time, the second gas column 300 can isolate the two types of sample addition liquids to prevent them from mixing.

[0173] S360 , after the liquid aspiration is completed, the injection unit 111 controls the sample addition needle 112 to discharge the sample addition liquid in the sample addition needle 112 into the reaction container 400 .

[0174] After the sample adding needle 112 completes the aspiration of the sample liquid, the sample adding needle 112 is controlled to move to the top of the reaction container 400 to discharge each sample liquid into the reaction container 400. That is, each type of sample liquid is aspirated respectively and then added to the reaction container 400 at one time, and the sample liquid can also be mixed at the same time, that is, the sample adding needle 112 continuously aspirates samples.

[0175] Moreover, the sample needle 112 can add at least two types of sample liquids into the reaction container 400 at one time. The adjacent sample liquids are isolated by the second gas column 300, and before sucking another type of sample liquid, the outer wall of the sample needle 112 needs to be cleaned to avoid cross contamination.

[0176] In this embodiment, after the sample adding needle 112 absorbs a reagent, only the outer wall of the sample adding needle 112 is cleaned, and then, a second gas column 300 is established in the sample adding needle 112. The sample adding needle 112 then absorbs the next component of the reagent or sample. After the required reagents and samples are sucked into the sample adding needle 112, they are all added to the reaction container 400 at one time, which can also play the role of mixing the sample and the reagent.

[0177] It is worth noting that the order in which the sample needle 112 draws the sample and each component reagent can be set according to the model of the test item. Generally, the sample is drawn last. In this way, after being transferred to the reaction container 400, the sample is first added to the reaction container 400, and then the reagents of each component are added to the reaction container 400 respectively and mixed with the sample.

[0178] In the present application, there are two ways of adding samples to the sample needle 112. One is that after the sample needle 112 absorbs a sample liquid and transfers it to the reaction container 400, the sample needle 112 is cleaned and the first gas column 200 is established, and then another sample liquid is absorbed and transferred to the reaction container 400. The other is that the sample needle 112 continuously absorbs multiple sections of sample liquid, establishes the first gas column 200 before absorbing the sample liquid, and uses the second gas column 300 to isolate adjacent sample liquids. After the liquid absorption is completed, the sample needle 112 is transferred to the top of the reaction container 400.

[0179] It is worth noting that when the sample needle 112 discharges the sample liquid into the reaction container 400 in the above two ways, it is discharged at the second preset speed, so that the sample liquid can be controlled to flow in a laminar flow, and at the same time, the dynamic stability of the sample liquid during the discharge is ensured, and the first gas column 200 can play an isolation role. The second preset speed can be obtained by experimenting in the same way as the first preset speed, and will not be repeated here.

[0180] Optionally, the sample needle 112 absorbs the sample liquid at a third preset speed, and the third preset speed ranges from 40 μL / s to 80 μL / s. In this way, the sample liquid absorption speed can be guaranteed, and turbulence in the sample needle 112 can be avoided, thereby ensuring the stability of the sample liquid absorption.

[0181] The third preset speed can also be obtained by experimenting in the same manner as the first preset speed, which will not be described in detail here. Moreover, when the sample adding needle 112 absorbs the sample adding liquid at the sample adding position, the height control of the sample adding needle 112 generally adopts liquid level detection or liquid level decreases with the amount, so as to control a small section of the needle tip 1123 to enter below the liquid level to be absorbed, thereby reducing the liquid hanging on the needle tip 1123.

[0182] In one embodiment, the amount of liquid discharged from the injection unit 111 into the reaction container 400 is the sum of the amount of liquid suction and the predetermined amount. The amount of liquid suction is the amount of liquid suctioned by the sample needle 112, or the amount of liquid suction is the sum of the amount of liquid suctioned by the sample needle 112 and the second predetermined amount of the second gas column 300. The second predetermined amount is less than the first predetermined amount.

[0183] That is, when the injection unit 111 discharges the sample liquid into the reaction container 400, it is necessary to discharge a predetermined amount more than the sample liquid absorbed by the sample needle 112. The predetermined amount can be greater than the return error and ensure that the sample liquid is completely discharged. Optionally, the range of the predetermined amount is 5 μL to 10 μL. Exemplarily, the predetermined amount is 5 μL to ensure that the sample liquid is completely discharged from the sample needle 112.

[0184] When the sample needle 112 transfers the sample and the reagent independently, the amount of liquid discharged from the sample needle 112 to the reaction container 400 is the sum of the amount of liquid sucked by the sample needle 112 and the predetermined amount. When the sample needle 112 sucks multiple sections of liquid, the amount of liquid discharged from the sample needle 112 to the reaction container 400 is the sum of the amount of liquid sucked by the sample needle 112, the second preset amount of the second gas column 300, and the predetermined amount.

[0185] It should be noted that the second preset amount of the second gas column 300 is not limited in principle, as long as the second gas column 300 can achieve isolation of adjacent sample liquids. When the second preset amount is less than the first preset amount, the time for the sample needle 112 to establish the second gas column 300 can be reduced, thereby improving the operating efficiency of the instrument. Optionally, the second preset amount ranges from 3 μL to 10 μL.

[0186] Referring to Figures 1, 8 and 9, Figure 9 is a schematic diagram of the distribution of each sample addition liquid and gas column in the sample addition needle 112 after the sample addition method shown in Figure 8 is used. In a specific example, after the sample addition needle 112 is cleaned, a first preset amount of 30 μL of gas is sucked at a first preset speed of 80 μL / s to establish a first gas column 200, and the cleaning liquid T is located above the first gas column 200. Subsequently, the sample addition needle 112 sucks 70 μL of the first component reagent R1.

[0187] Then, after cleaning the outer wall of the sample adding needle 112, the sample adding needle 112 draws a second preset amount of gas (such as 5 μL, etc.) to establish the second gas column 300, and the sample adding needle 112 draws 80 μL of the second component reagent R2. After cleaning the outer wall of the sample adding needle 112, the sample adding needle 112 draws a second preset amount of gas (such as 3 μL, etc.) to establish the second gas column 300. The sample adding needle 112 draws 5 μL of the sample S. Subsequently, the sample adding needle 112 moves to the top of the reaction container 400, and adds the sample S, the second component reagent R2, and the first component reagent R1 into the reaction container 400 at a second preset speed.

[0188] It is worth noting that the volume flow rate of the sample addition liquid when the sample addition needle 112 controls the sample addition liquid to be discharged is a constant value. However, the flow rate of the sample addition liquid is different when it flows through different cross sections of the sample addition needle 112. Exemplarily, the flow rate u1 at the needle tube 1121 is greater than the flow rate u2 at the diameter reducing section 1122, as shown in FIG9 .

[0189] After the sample S, the second component reagent R2, and the first component reagent R1 in the reaction container 400 are incubated for a period of time (e.g., 9 minutes), the sample needle 112 is cleaned, and a first preset amount of 30 μL of gas is sucked at a first preset speed of 80 μL / s to establish the first gas column 200, and the cleaning liquid T is located above the first gas column 200. Subsequently, the sample needle 112 sucks 70 μL of the third component reagent R3 and transfers it to the reaction container 400. In this way, the sample and the reagents of each component are added to the reaction container 400.

[0190] It is worth noting that the amount of sample and each component reagent absorbed by the sample needle 112 can be set according to the model of the test item. In the sample addition operation of one embodiment, the amount of sample absorbed by the sample needle 112 is 5 μL to achieve the transfer of trace samples. Of course, in other embodiments of the present application, the sample needle 112 can also transfer other amounts of samples as required. Accordingly, the reagents are added according to the samples of the test items, which will not be repeated here.

[0191] In the sample addition control method of the present application, after cleaning the sample addition needle 112, the injection unit 111 can control the sample addition needle 112 to absorb gas to establish a first gas column 200 in the sample addition needle 112, and the first gas column 200 can isolate the cleaning liquid and the sample addition liquid to prevent the cleaning liquid and the sample addition liquid from contacting and mixing with each other. Moreover, when the injection unit 111 establishes the first gas column 200 in the sample addition needle 112, the injection unit 111 can control the sample addition needle 112 to absorb a first preset amount of gas at a first preset speed to establish a stable and reliable first gas column 200 in the sample addition needle 112, and minimize the amount of residual liquid on the inner wall of the sample addition needle 112 after the first gas column 200 is established, thereby preventing the sample addition liquid from mixing with the residual liquid, improving the accuracy and repeatability of micro-sampling, and thereby improving the accuracy of the measurement results.

[0192] Referring to FIG. 1 , the present application also provides a sample addition system 100, which is applied to a sample addition control method as in any of the above embodiments. It is worth noting that the structure of the sample addition system 100 has been mentioned above and will not be repeated here. When the sample addition system 100 of the present application is operated according to the above sample addition control method, the accuracy and precision of micro-liquid sample addition can be improved, thereby ensuring the accuracy of the measurement results.

[0193] It should be noted that, regardless of whether the sample needle 112 continuously absorbs the sample liquid or absorbs the sample liquid once, in order to prevent the residual liquid on the outer wall of the sample needle 112 from contaminating the next sample liquid, the present application can use the cleaning pool 133 alone to clean the outer wall of the sample needle 112, and can also use the inner wall cleaning component 120 to clean the inner wall when the sample needle 112 cleans the outer wall, and collect the waste liquid through the cleaning pool 133.

[0194] In one embodiment, the cleaning pool 133 includes a cleaning body and a plurality of cleaning holes arranged on the cleaning body, the cleaning body connects the outer wall cleaning pipeline 131 and the drainage pipeline 135, the plurality of cleaning holes are evenly arranged around the central axis of the cleaning body, and the cleaning holes are inclined relative to the central axis of the cleaning body and aligned with the central axis of the cleaning body.

[0195] The cleaning hole is arranged obliquely downward, and one end of the cleaning hole is aligned with the central axis of the cleaning body. In this way, after the sample adding needle 112 is inserted into the cleaning pool, the needle tip 1123 of the sample adding needle 112 can contact the intersection center of the extension lines of multiple cleaning holes in the cleaning pool, so that one end of each cleaning hole can be aligned with the outer wall of the sample adding needle 112.

[0196] When cleaning, the outer wall cleaning valve 132 is opened, and the high-pressure cleaning liquid is sprayed downward from the cleaning hole, and the cleaning hole can spray the cleaning liquid on the outer wall of the sample needle 112. Moreover, the uniformly designed cleaning holes can ensure that the cleaning liquid cleans the outer wall of the sample needle 112 from 360 degrees. At this time, the sample needle 112 only needs to drop to the confluence center of each cleaning hole to clean the outer wall, so as to ensure the cleaning effect of the outer wall of the sample needle 112. The cleaning pool 133 collects waste liquid, and the waste liquid is pumped out through the connected negative pressure pump 134.

[0197] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0198] The above-described embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be construed as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent application shall be subject to the attached claims.

Claims

1. A sample addition control method, characterized in that: include: Clean the sample needle; The injection unit controls the sample adding needle to absorb a first preset amount of gas at a first preset speed and establishes a first gas column in the sample adding needle; The injection unit controls the sample adding needle to absorb the sample adding liquid. After the sample adding liquid is absorbed, the injection unit controls the sample adding needle to discharge the sample adding liquid into the reaction container at a second preset speed.

2. The sample addition control method according to claim 1, characterized in that: The step of cleaning the sample adding needle at least comprises: Controlling the sample adding needle to move to the cleaning tank to clean the sample adding needle; When cleaning the inner wall of the sample injection needle, controlling the injection unit to establish a preset amount of cleaning liquid; When the inner wall cleaning valve is closed, the injection unit controls the sample addition needle to discharge the preset amount of cleaning liquid.

3. The sample addition control method according to claim 2, characterized in that: The step of cleaning the sample adding needle at least includes: When the sample adding needle discharges the preset liquid volume, the cleaning pool is controlled to maintain negative pressure to pump out the waste liquid, so that the cleaning liquid in the sample adding needle is flush with the needle tip of the sample adding needle.

4. The sample addition control method according to claim 1, characterized in that: The sample addition control method includes at least one of the following items: Item 1, the first preset speed ranges from 60 μL / s to 80 μL / s; Item 2, the range of the second preset speed is 80 μL / s to 160 μL / s; The third item, the second preset speed is greater than the first preset speed; Item 4: the first preset amount is greater than the volume of the tip of the sample adding needle + the volume of the reduced diameter section of the sample adding needle × 2; Fifthly, the sample adding needle absorbs the sample adding liquid at a third preset speed, and the range of the third preset speed is 40 μL / s to 80 μL / s.

5. The sample addition control method according to any one of claims 1 to 4, characterized in that: The first preset speed and the second preset speed are obtained by the following formula: Wherein, V is the linear flow velocity of the liquid in the sample needle, d is the inner diameter of the sample needle, l is the length of the sample pipe between the injection unit and the sample needle, R is the pipe resistance coefficient of the sample pipe, and Re is the Reynolds number of the liquid in the sample needle.

6. The sample addition control method according to claim 5, characterized in that: The sample addition control method further comprises: When the Reynolds number of the liquid in the sample injection needle is less than 2000, calculating the linear flow rate of the liquid; converting the linear flow rate into a volumetric flow rate; Selecting multiple groups of data smaller than the volume flow rate as experimental flow rates, and selecting multiple groups of inhaled amounts of the first gas column as experimental gas amounts; A repeatability experiment is performed on each of the experimental flow rates and each of the experimental gas volumes to determine the first preset speed and the first preset volume.

7. The sample addition control method according to any one of claims 1 to 4, characterized in that: The sample addition control method further comprises: The sample addition needle independently transfers each sample addition liquid into the reaction container; After transferring the previous sample liquid and before aspirating the next sample liquid, the sample needle is cleaned, and the first gas column is established after the sample needle is cleaned; or, The sample addition control method further comprises: After the sample adding needle is controlled to absorb a sample adding liquid, the outer wall of the sample adding needle is cleaned; The injection unit controls the sample adding needle to absorb a second preset amount of gas and establishes a second gas column in the sample adding needle; Controlling the sample adding needle to absorb another sample adding liquid; After the liquid aspiration is completed, the injection unit controls the sample addition needle to discharge each sample addition liquid in the sample addition needle into the reaction container.

8. The sample addition control method according to claim 7, characterized in that: The amount of sample liquid discharged from the injection unit into the reaction container is the sum of the aspirated amount and the predetermined amount; The aspirated amount is the amount of the sample liquid aspirated by the sample needle, or the aspirated amount is the sum of the amount of the sample liquid aspirated by the sample needle and a second preset amount of the second gas column, and the second preset amount is smaller than the first preset amount.

9. A sample adding system, characterized in that: Applied to the sample addition control method according to any one of claims 1 to 8, the sample addition system comprises an injection component, an inner wall cleaning component, an outer wall cleaning component and a cleaning pump; The injection assembly comprises an injection unit, a sample addition needle and a sample addition pipeline, wherein the sample addition pipeline connects the injection unit and the sample addition needle; The inner wall cleaning assembly is used to clean the inner wall of the injection needle, and comprises an inner wall cleaning pipeline and an inner wall cleaning valve, wherein the inner wall cleaning pipeline is connected with the cleaning pump and the injection unit, and the inner wall cleaning valve is arranged on the inner wall cleaning pipeline to control the on-off of the inner wall cleaning pipeline; The outer wall cleaning component includes an outer wall cleaning pipeline, a cleaning tank, a negative pressure pump, a discharge pipeline and an outer wall cleaning valve. The outer wall cleaning pipeline connects the cleaning pump and the cleaning tank, the discharge pipeline connects the cleaning tank, the outer wall cleaning valve is arranged on the outer wall cleaning pipeline to control the opening and closing of the outer wall cleaning pipeline, and the negative pressure pump is arranged on the discharge pipeline to draw negative pressure into the cleaning tank.

10. The sample loading system according to claim 9, characterized in that: The cleaning pool includes a cleaning body and a plurality of cleaning holes arranged in the cleaning body, the cleaning body is connected with the outer wall cleaning pipeline and the drainage pipeline, and the plurality of cleaning holes are evenly arranged around the central axis of the cleaning body; The cleaning hole is arranged obliquely relative to the central axis of the cleaning body and is aligned with the central axis of the cleaning body.

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