Method for improving sterilization and filtration loading capacity of lactoferrin liquid
By adopting the steps of coarse filtration, ultrafiltration desalination and concentration, refined filtration and sterilization filtration in lactoferrin liquid, the problem of difficult removal of particulate impurities in lactoferrin extraction solution is solved, significantly improving the sterilization filtration load, reducing production costs, and improving the taste of the product.
Patent Information
- Application Number
- CN202311088045.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-08-28
- Publication Date
- 2025-02-28
AI Technical Summary
The lactoferrin extraction solution contains a large amount of particle impurities, resulting in low membrane processing during sterilization and filtration, high production cost, and large volume and long time during filtration and sterilization, which affects the taste of the product.
The steps of crude filtration, ultrafiltration desalination concentration, refined filtration and sterilization filtration are adopted to remove impurities in the lactoferrin liquid through the combination of different membrane filtration technologies, and concentrate and desalinate them to increase the load of sterilization filtration.
It significantly increases the sterilization and filtration load of lactoferrin liquid, shortens the processing time, reduces production costs, and effectively removes salt in the solution, improving the taste of the product.
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Figure BDA0004416750830000041
Abstract
Description
Technical Field
[0001] The invention relates to a method for improving the sterilization filtration loading capacity of a lactoferrin liquid, and belongs to the technical field of dairy products. Background Art
[0002] The lactoferrin extraction solution contains a large amount of particulate impurities, which cannot effectively pass through the sterilization membrane during the sterilization filtration process. The membrane processing capacity during the production process is low and the production cost is high. Conventional filtration treatment does not perform ultrafiltration concentration and desalination treatment on lactoferrin. The volume is large and the processing time is long during the filtration and sterilization process, and residual salt will affect the taste of the product. After concentration, the concentration of particulate impurities is also enriched, making it impossible for them to pass through the sterilization membrane. The two technologies used alone cannot improve the filtration load and production efficiency. Summary of the invention
[0003] In order to solve the above technical problems, the object of the present invention is to provide a method for sterilizing and filtering a lactoferrin solution, which method can have a higher sterilizing and filtering load.
[0004] To achieve the above object, the present invention provides a method for improving the sterilization filtration loading capacity of lactoferrin solution, which comprises the steps of coarse filtration, ultrafiltration desalination concentration, fine filtration and sterilization filtration of the lactoferrin solution.
[0005] In the above method, the steps of coarse filtration, ultrafiltration desalination concentration, fine filtration and sterilizing filtration are performed in sequence.
[0006] In the above method, preferably, the concentration of the lactoferrin solution is 5%-15%.
[0007] In the above method, large particle impurities can be removed by coarse filtration. Preferably, the filter used in the coarse filtration is a filter with a filtration pore size of 2.5-4.0 microns.
[0008] In the above method, preferably, the ultrafiltration desalination and concentration comprises: ultrafiltration treatment of the lactoferrin solution after coarse filtration, the concentration multiple is 5-10 times, after obtaining the concentrated solution, adding water 2-10 times the volume of the concentrated solution thereto, and ultrafiltration and concentration again 5-10 times to obtain the lactoferrin concentrated solution.
[0009] In the above method, preferably, the ultrafiltration membrane device used in the ultrafiltration has a molecular weight cutoff of 5000-10000Da.
[0010] In the above method, preferably, the operating pressure of the ultrafiltration membrane device is 0.2-2.0 bar (preferably 0.5-2.0 bar).
[0011] In the above method, preferably, the ultrafiltration desalting and concentration process is repeated 3-6 times to obtain a lactoferrin concentrate.
[0012] In the above method, small particle impurities can be removed by fine filtration; preferably, the fine filtration is performed using a filter with a filtration pore size of 0.3-0.6 microns.
[0013] In the above method, preferably, the operating pressure of the sterilizing filtration is 0-2.5 bar.
[0014] In the above method, preferably, the sterilizing filtration is performed using a sterile filtration device.
[0015] According to a specific embodiment of the present invention, preferably, the method comprises the following steps:
[0016] (1) Coarse filtration of lactoferrin: Coarse filtration of the lactoferrin solution was performed at 4-25° C. using a filter with a pore size of 2.5-4.0 μm;
[0017] (2) Ultrafiltration desalination and concentration: ultrafiltration treatment is performed on the lactoferrin solution after coarse filtration at 4-25° C., and the concentration multiple is 5-10 times. After obtaining the concentrated solution, water with a volume of 2-10 times of the concentrated solution is added thereto, and ultrafiltration and concentration is performed again by 5-10 times. The process is repeated 3-6 times to obtain the lactoferrin concentrated solution. The ultrafiltration membrane equipment used is an ultrafiltration membrane equipment with a molecular weight cutoff of 5000-10000Da, and the operating pressure is 0.2-2.0bar. During the operation, the inlet and outlet temperatures of the membrane filtration equipment are 4-25° C.;
[0018] (3) Fine filtration of lactoferrin concentrate: Fine filtration of the concentrated and desalted lactoferrin solution using a filter having a pore size of 0.3-0.6 μm at 4-25° C. to obtain finely filtered lactoferrin solution;
[0019] (4) Sterile filtration: The finely filtered lactoferrin solution is subjected to sterile filtration treatment to obtain a sterile lactoferrin solution; wherein, a sterile sterile filtration device is used, the operating pressure is 0-2.5 bar, and the inlet and outlet temperatures of the sterile filtration device are 4-25°C.
[0020] The present invention can remove impurities in lactoferrin solution through the combination of different membrane filtration technologies, concentrate and desalt the lactoferrin solution, increase the loading capacity of the lactoferrin solution during sterilization filtration, and remove excess salt in the solution. DETAILED DESCRIPTION
[0021] In order to have a clearer understanding of the technical features, purposes and beneficial effects of the present invention, the technical solution of the present invention is now described in detail below, but it should not be construed as limiting the applicable scope of the present invention.
[0022] Example 1
[0023] This embodiment provides a method for increasing the sterilization filtration capacity of lactoferrin solution, comprising the following specific steps:
[0024] (1) Coarse filtration of lactoferrin: Coarse filtration of the lactoferrin solution was performed at 10-15°C using a filter with a pore size of 3.0 μm;
[0025] (2) Ultrafiltration desalination concentration (UF concentration): The lactoferrin solution after coarse filtration was subjected to ultrafiltration treatment at 10-15° C., and the concentration multiple was 10 times. After obtaining the concentrated solution, water with a volume twice that of the concentrated solution was added thereto, and ultrafiltration was performed again to concentrate the solution 3 times. The process was repeated 5 times to obtain the lactoferrin concentrated solution. The ultrafiltration membrane device used was an ultrafiltration membrane device with a molecular weight cutoff of 10,000 Da, and the operating pressure was 0.5 bar. During the operation, the inlet and outlet temperatures of the membrane filtration device were 10-15° C.;
[0026] (3) Fine filtration of lactoferrin concentrate: Fine filtration of the concentrated and desalted lactoferrin solution using a filter with a pore size of 0.5 μm at 10-15° C. to obtain finely filtered lactoferrin solution;
[0027] (4) Sterile filtration: The finely filtered lactoferrin solution is subjected to sterile filtration treatment to obtain a sterile lactoferrin solution; wherein, a sterile sterile filtration device is used, the operating pressure is 1.5 bar, and the inlet and outlet temperatures of the sterile filtration device are 10-15°C.
[0028] (5) Aseptic filling: Aseptically fill the product liquid into the corresponding packaging material.
[0029] Comparative Example 1
[0030] This comparative example provides a method for sterilizing and filtering a lactoferrin solution, comprising the following specific steps:
[0031] (1) Ultrafiltration desalination concentration (UF concentration): The lactoferrin solution was ultrafiltered at 10-15° C., and the concentration multiple was 10 times. After obtaining the concentrated solution, water with a volume twice that of the concentrated solution was added thereto, and the solution was ultrafiltered again and concentrated 3 times. The process was repeated 5 times to obtain the lactoferrin concentrated solution. The ultrafiltration membrane device used was an ultrafiltration membrane device with a molecular weight cutoff of 10,000 Da, and the operating pressure was 0.5 bar. During the operation, the inlet and outlet temperatures of the membrane filtration device were 10-15° C.;
[0032] (2) Coarse filtration of lactoferrin: Coarse filtration of the lactoferrin solution after ultrafiltration desalination concentration (UF concentration) was performed at 10-15°C using a filter with a pore size of 3.0 μm;
[0033] (3) Fine filtration of lactoferrin concentrate: Fine filtration of the concentrated and desalted lactoferrin solution using a filter with a pore size of 0.5 μm at 10-15° C. to obtain finely filtered lactoferrin solution;
[0034] (4) Sterile filtration: The finely filtered lactoferrin solution is subjected to sterile filtration treatment to obtain a sterile lactoferrin solution; wherein, a sterile sterile filtration device is used, the operating pressure is 2.0 bar, and the inlet and outlet temperatures of the sterile filtration device are 10-15°C.
[0035] (5) Aseptic filling: Aseptically fill the product liquid into the corresponding packaging material.
[0036] Comparative Example 2
[0037] This comparative example provides a method for sterilizing and filtering a lactoferrin solution, comprising the following specific steps:
[0038] (1) Coarse filtration of lactoferrin: Coarse filtration of the lactoferrin solution was performed at 10-15°C using a filter with a pore size of 3.0 μm;
[0039] (2) Fine filtration of lactoferrin concentrate: fine filtration of the lactoferrin concentrate after coarse filtration is performed at 10-15° C. using a filter with a pore size of 0.5 μm to obtain a finely filtered lactoferrin concentrate;
[0040] (3) Ultrafiltration desalination concentration (UF concentration): The lactoferrin solution after fine filtration was subjected to ultrafiltration treatment at 10-15° C., and the concentration multiple was 10 times. After obtaining the concentrated solution, water twice the volume of the concentrated solution was added thereto, and ultrafiltration was performed again to concentrate 3 times. The process was repeated 5 times to obtain the lactoferrin concentrated solution. The ultrafiltration membrane equipment used was an ultrafiltration membrane equipment with a molecular weight cutoff of 10,000 Da, the operating pressure was 1.0 bar, and the inlet and outlet temperatures of the membrane filtration equipment during operation were 10-15° C.;
[0041] (4) Sterile filtration: The finely filtered lactoferrin solution is subjected to sterile filtration treatment to obtain a sterile lactoferrin solution; wherein, a sterile sterile filtration device is used, the operating pressure is 2.0 bar, and the inlet and outlet temperatures of the sterile filtration device are 10-15°C.
[0042] (5) Aseptic filling: Aseptically fill the product liquid into the corresponding packaging material.
[0043] Comparative Example 3
[0044] This comparative example provides a method for sterilizing and filtering a lactoferrin solution, comprising the following specific steps:
[0045] Ultrafiltration desalination concentration (UF concentration): at 10-15°C, the lactoferrin solution is subjected to ultrafiltration treatment, the concentration multiple is 10 times, after obtaining the concentrated solution, water twice the volume of the concentrated solution is added thereto, ultrafiltration is performed again to concentrate 3 times, and the process is repeated 5 times to obtain the lactoferrin concentrated solution; the ultrafiltration membrane equipment used is an ultrafiltration membrane equipment with a molecular weight cutoff of 10000Da, the operating pressure is 1.0bar, and the inlet and outlet temperatures of the membrane filtration equipment during operation are 10-15°C;
[0046] Aseptic filling: Aseptically fill the product liquid into the corresponding packaging material.
[0047] The test results of the sterilization membrane filtration capacity of Example 1 and Comparative Examples 1-3 are shown in Table 1.
[0048] Table 1
[0049]
[0050] It can be seen from the data in Table 1 that the method of Example 1 can obtain a filtration load significantly higher than that of Comparative Examples 1-3.
[0051] The test results of salt content before and after ultrafiltration concentration desalination in Example 1 and Comparative Examples 1-3 are shown in Table 2.
[0052] Table 2
[0053] serial number Salt content before ultrafiltration concentration mol / L Salt content after ultrafiltration concentration mol / L Example 1 1.08 0.03-0.05 Comparative Example 1 1.06 0.03-0.05 Comparative Example 2 1.05 0.03-0.05 Comparative Example 3 1.08 0.03-0.05
[0054] It can be seen from the data in Table 2 that the salt content of the feed solution can be significantly reduced through ultrafiltration concentration and elution treatment.
Claims
1. A method for increasing the sterilization filtration loading capacity of a lactoferrin solution, comprising the steps of coarse filtration, ultrafiltration desalination concentration, fine filtration, and sterilization filtration of the lactoferrin solution.
2. The method according to claim 1, wherein: The concentration of the lactoferrin solution is 5%-15%.
3. The method according to claim 1, wherein: The filter used for the coarse filtration is a filter with a filtration pore size of 2.5-4.0 microns.
4. The method according to claim 1, wherein: The ultrafiltration desalination and concentration comprises: ultrafiltration treatment of the lactoferrin solution after coarse filtration, the concentration multiple is 5-10 times, after obtaining the concentrated solution, water 2-10 times the volume of the concentrated solution is added thereto, and ultrafiltration and concentration is performed again by 5-10 times to obtain the lactoferrin concentrated solution.
5. The method according to claim 4, wherein: The ultrafiltration membrane device used in the ultrafiltration has a molecular weight cutoff of 5000-10000Da.
6. The method according to claim 5, wherein: The operating pressure of the ultrafiltration membrane device is 0.2-2.0 bar (preferably 0.5-2.0 bar).
7. The method according to claim 4, wherein: The ultrafiltration desalting and concentration process is repeated 3-6 times to obtain a lactoferrin concentrate.
8. The method according to claim 1 or 4, wherein: The fine filtration is performed using a filter with a pore size of 0.3-0.6 microns.
9. The method according to claim 1 or 8, wherein: The operating pressure of the sterilizing filtration is 0-2.5 bar; Preferably, the sterilizing filtration is performed using a sterile filtration device.
10. The method according to claim 1 or 4, wherein: The method comprises the following steps: (1) Coarse filtration of lactoferrin: Coarse filtration of the lactoferrin solution was performed at 4-25° C. using a filter with a pore size of 2.5-4.0 μm; (2) Ultrafiltration desalination and concentration: The lactoferrin solution after the coarse filtration is subjected to ultrafiltration treatment at 4-25° C., and the concentration multiple is 5-10 times. After obtaining the concentrated solution, water with a volume of 2-10 times of the concentrated solution is added thereto, and ultrafiltration and concentration is performed again by 5-10 times. The process is repeated 3-6 times to obtain the lactoferrin concentrated solution. The ultrafiltration membrane device used is an ultrafiltration membrane device with a molecular weight cut-off of 5000-10000Da, and the operating pressure is 0.2-2.0bar (preferably 0.5-2.0bar). The inlet and outlet temperatures of the membrane filtration device during operation are 4-25° C.; (3) Fine filtration of lactoferrin concentrate: Fine filtration of the concentrated and desalted lactoferrin solution using a filter having a pore size of 0.3-0.6 μm at 4-25° C. to obtain finely filtered lactoferrin solution; (4) Sterile filtration: The finely filtered lactoferrin solution is subjected to sterile filtration treatment to obtain a sterile lactoferrin solution; wherein, a sterile sterile filtration device is used, the operating pressure is 0-2.5 bar, and the inlet and outlet temperatures of the sterile filtration device are 4-25°C.