Efficient PCV2 virus proliferation and purification system

Abstract

The invention discloses an efficient PCV2 virus proliferation and purification system which comprises a linear actuator integrated operation table, a linear actuator is fixedly installed on a bearing platform of the integrated operation table, a mechanical arm is fixedly installed on a sliding platform of the linear actuator, and the mechanical arm is located between hidden machine covers of two hidden protectors. Hidden machine covers of the two hidden protectors are fixedly installed on a bearing platform of the integrated operation table in a mirror symmetry mode, and proliferation equipment or purification equipment is fixedly installed in the two hidden protectors respectively. Efficient control and biological safety measures are integrated, and through cooperative work of the linear actuator and the operation table, the grabbing efficiency of the mechanical arm is greatly improved, and manual intervention is reduced. And the hidden protector closes the equipment, so that the biological safety is guaranteed, and virus leakage is prevented. The cavity design optimizes resource management and reduces waste liquid pollution. Modular equipment is convenient to maintain, the flexibility and adaptability of the system are enhanced, and various experiment requirements are met.

Description

Technical Field

[0001] The invention relates to the technical field of porcine circovirus proliferation and purification, in particular to a highly efficient PCV2 virus proliferation and purification system. Background Art

[0002] PCV2 infection can cause porcine circovirus-associated disease (PCVAD), including postweaning multisystemic wasting syndrome (PMWS), dermatitis and nephropathy syndrome (PDNS), reproductive disorders, respiratory diseases and intestinal diseases, etc., causing huge economic losses to the global pig industry. PCV2 infection can also induce immunosuppression in pigs, increase susceptibility to other pathogens, and reduce the immune effect of vaccines.

[0003] At present, the proliferation of PCV2 virus is mainly carried out through cell culture system, and the commonly used host cells include porcine kidney epithelial cells (PK-15). During the cell culture process, PCV2 virus completes its life cycle through steps such as adsorption, invasion, replication and release. In order to obtain high titer PCV2 virus, it is necessary to optimize the culture conditions, including culture medium composition, temperature, pH value and culture time.

[0004] Purification of PCV2 virus is a complex process that aims to isolate high-purity virus particles from cell culture. Common purification methods include differential centrifugation, density gradient centrifugation, ultrafiltration and chromatography.

[0005] In the field of virology research and biopharmaceuticals, efficient proliferation and purification of PCV2 (porcine circovirus type 2) is a key step to ensure the quality of vaccines and diagnostic reagents. However, in the layout of traditional laboratories or production workshops, proliferation equipment and purification equipment are often dispersed, which not only increases the distance between equipment, but also makes the virus stock solution or samples face a very high risk of contamination during transportation. Due to the high sensitivity and strong infectivity of the virus, any slight contamination may have a serious impact on the experimental results or product quality, and even cause a biosafety accident.

[0006] Specifically, during the transportation process, the stock solution or sample may be damaged due to poor container sealing, careless operation or microbial contamination in the environment. In addition, the decentralized equipment layout also leads to some other problems such as extended transportation time and increased risk of sample exposure in an open environment. These factors work together to reduce the overall efficiency of virus proliferation and purification, increase costs, and pose a major safety hazard.

[0007] Therefore, it is very necessary to invent an efficient PCV2 virus proliferation and purification system. Summary of the invention

[0008] The present invention provides an efficient PCV2 virus proliferation and purification system, and the present invention provides the following technical solutions: comprising a linear actuator integrated operating table, a mechanical arm, a hidden protector, a proliferation device and a purification device, wherein the linear actuator is fixedly mounted on the bearing platform of the integrated operating table, a mechanical arm is fixedly mounted on the sliding platform of the linear actuator, and the mechanical arm is located between two hidden machine covers of the hidden protectors, and the two hidden machine covers of the hidden protectors are fixedly mounted on the bearing platform of the integrated operating table in a mirror-symmetrical manner, wherein the proliferation device or the purification device is fixedly mounted in the two hidden protectors respectively;

[0009] A flexible clamp is fixedly mounted at the output end of the robotic arm, and is used to grab tools used in the proliferation or purification process.

[0010] Preferably, a guide rail frame is fixedly mounted on the bearing platform, a storage cover is fixedly mounted on the guide rail frame, an isolation transparent plate is slidably mounted inside the guide rail frame and the storage cover, and an operation panel is fixedly mounted on the outer side of the guide rail frame.

[0011] Preferably, the insulating transparent plate is provided with a raised handle, and a sealing groove is provided below the insulating transparent plate, and the sealing groove is opened on the bearing platform.

[0012] Preferably, a back plate is fixedly mounted on the bearing platform, and a temperature control device is fixedly mounted on the back plate and the two hidden protectors, and the temperature control device is used to control the temperature of the sealed space below.

[0013] Preferably, a disinfection pipeline is fixedly installed on the back plate, the two hidden protectors and the guide rail frame, the disinfection pipeline is located below the temperature control device, and a disinfection lamp is fixedly installed on the inner side of the disinfection pipeline, and the disinfection lamp is used to disinfect and sterilize the space below.

[0014] Preferably, the disinfection pipeline and the disinfection lamp are not arranged at the exhaust port of the temperature control device, so as not to interfere with the direction of the airflow discharged from the temperature control device.

[0015] Preferably, a plurality of disinfection spray heads are arranged below the disinfection pipeline, and the disinfection pipeline is a pipe structure with a square outside and a round inside.

[0016] Preferably, the integrated operating table includes a transverse slide groove, a waste liquid collection groove, a cover plate, and an integrated box, wherein the transverse slide groove and the waste liquid collection groove are opened on the surface of the bearing platform, wherein a linear actuator is fixedly installed on the inner side of the transverse slide groove; a cover plate is embedded in the opening of the waste liquid collection groove, and the cover plate and the waste liquid collection groove together form a waste liquid collection channel, and a bearing platform is fixedly installed above the integrated box.

[0017] Preferably, a plurality of cavities are provided in the integrated box, and worktables are provided at the openings of two of the cavities. The worktables are rotatably mounted on the inner side of the carrying platform, and the output end of the servo motor fixedly mounted on the carrying platform is fixed to one end of the corresponding worktable. The servo motor is used to control the opening and closing of the worktable.

[0018] Preferably, the cavity is divided into a waste liquid collection chamber, a tool placement chamber and a disinfection chamber, and the tools required for virus proliferation or purification are placed inside the tool placement chamber, wherein workbenches are provided at the openings of the tool placement chamber and the disinfection chamber, and a positioning seat is fixedly installed above one of the workbenches, and the positioning seat is used to position the test tube rack.

[0019] Preferably, the waste liquid collection chamber and the disinfection chamber of the cavity are connected to the collection box for storing waste liquid internally through a three-way pipeline and a pump body, and the collection box for storing disinfectant internally is connected to the disinfection chamber of the cavity through a pipeline and a pump body.

[0020] Preferably, the hidden protector also includes a hidden groove, a carrier packaging plate and a driving component. A plurality of hidden grooves are arranged in the hidden hood. A carrier packaging plate is rotatably installed at the opening of the hidden groove. Each of the carrier packaging plates is fixed to the corresponding output end of the driving component, and the driving component is fixedly installed on the outside of the hidden hood.

[0021] Preferably, a proliferation device and a purification device are fixedly installed on the inner side of each carrier packaging plate, and the proliferation device and the purification device are arranged in a hidden groove.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. The integrated control of the integrated operating table and the linear actuator of the present invention enables the robotic arm to quickly and accurately grasp and place the tools required in the proliferation and purification process, greatly reducing manual intervention and operation time, and significantly improving the overall operation efficiency.

[0024] 2. The design of the hidden protector of the present invention encloses the proliferation equipment and purification equipment in a hidden hood, effectively isolating the potential dangers in the virus handling process, not only protecting the operators from the risk of infection, but also greatly reducing the possibility of virus leakage, ensuring biosafety.

[0025] 3. The temperature control device and disinfection pipeline of the present invention provide the best environmental conditions for virus proliferation and purification. The temperature control device accurately controls the temperature, while the disinfection pipeline and disinfection lamp regularly kill viruses and bacteria, ensuring that the temperature and sanitary conditions in the operating space are in the optimal state, ensuring the safety and cleanliness of the working environment.

[0026] 4. The waste liquid collection chamber, tool placement chamber, disinfection chamber and other cavities designed inside the integrated operating table of the present invention realize the effective management of resources and centralized treatment of waste liquid. This design not only improves work efficiency, but also significantly reduces the pollution of waste liquid to the environment and improves resource utilization efficiency.

[0027] 5. The proliferation device and purification device of the present invention are installed in a rotatable carrier packaging plate, and the opening and closing thereof are controlled by a driving component. This modular design not only simplifies the maintenance and replacement process of the equipment, but also improves the flexibility and scalability of the system, so that the system can easily adapt to different experimental and production requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0029] Figure 2 It is a structural schematic diagram of the integrated operating table of the present invention.

[0030] Figure 3 It is a partial cross-sectional structural schematic diagram of the integrated operating table of the present invention.

[0031] Figure 4 It is a schematic diagram of the bottom structure of the integrated operating table of the present invention.

[0032] Figure 5 It is a schematic diagram of the structure of the temperature control device of the present invention when it is not installed.

[0033] Figure 6 The present invention Figure 5 Another perspective structural diagram of .

[0034] Figure 7 It is a schematic diagram of the structure of the hidden protector of the present invention.

[0035] Figure 8 It is a schematic diagram of the structure of the hidden protector of the present invention when it is closed.

[0036] In the figure:

[0037] Linear actuator 1, integrated operating table 2, carrying platform 21, horizontal slide 22, sealing groove 23, waste liquid collection groove 24, cover plate 25, integrated box 26, robotic arm 3, hidden protector 4, hidden machine cover 41, hidden groove 42, carrier packaging plate 43, driving component 44, proliferation equipment 5, purification equipment 5A, guide rail frame 6, storage cover 7, isolation transparent plate 8, operation panel 9, temperature control equipment 10, pump body 11, three-way pipe 12, collection box 13, disinfection pipe 14, disinfection lamp 15, back plate 16. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] As attached Figure 1-8 As shown:

[0040] The present invention provides an efficient PCV2 virus proliferation and purification system, comprising a linear actuator 1, an integrated operating table 2, a mechanical arm 3, a hidden protector 4, a proliferation device 5 and a purification device 5A, wherein the linear actuator 1 is fixedly mounted on a carrying platform 21 of the integrated operating table 2, and the mechanical arm 3 is fixedly mounted on a sliding platform of the linear actuator 1, and the mechanical arm 3 is located between two hidden machine covers 41 of the hidden protectors 4, and the two hidden machine covers 41 of the hidden protectors 4 are fixedly mounted on the carrying platform 21 of the integrated operating table 2 in a mirror-symmetrical manner, wherein the proliferation device 5 or the purification device 5A is fixedly mounted in the two hidden protectors 4 respectively.

[0041] Furthermore, the robot arm 3 is a core operating component, and a flexible clamp is fixedly installed at its output end. This flexible clamp is flexible in design and can safely and accurately grasp various tools used in the proliferation or purification process, such as test tubes, pipettes, etc., greatly improving the accuracy and efficiency of the operation.

[0042] Furthermore, a guide rail frame 6 is installed on the carrying platform 21, and a storage cover 7 is fixed above the guide rail frame. An isolation transparent plate 8 is slidably installed in the guide rail frame 6 and the storage cover 7. The isolation transparent plate 8 not only provides a visual observation window during operation, but also facilitates the operator to push and pull through the raised handle on it. The sealing groove 23 below it ensures that the isolation transparent plate 8 can be effectively sealed when closed to prevent external pollution from entering the operating area.

[0043] Furthermore, in order to ensure the best environmental conditions for virus proliferation and purification, a temperature control device 10 is installed on the back plate 16 and the two hidden protectors 4. At the same time, in order to ensure the sterility of the operating space, a disinfection pipeline 14 is also fixed on the back plate 16, the hidden protector 4 and the guide frame 6, and a disinfection lamp 15 is installed inside the disinfection pipeline 14. The disinfection lamp 15 is started regularly, and the space is fully disinfected through the spray head below to ensure the cleanliness of the operating environment. It is specially designed that the position of the disinfection pipeline 14 and the disinfection lamp 15 avoids conflict with the exhaust port of the temperature control device 10, ensuring the normal operation of the temperature control system.

[0044] Furthermore, the integrated operating table 2 integrates multiple functions, including a horizontal slide 22, a waste liquid collection tank 24, a cover plate 25 and an integrated box 26. A plurality of cavities 28 are provided in the integrated box 26, which are used for waste liquid collection, tool placement and disinfection, respectively. The workbench 29 is opened and closed by a servo motor 20, which is convenient for taking and placing items. In particular, the positioning seat 21 in the tool placement cavity can accurately fix the test tube rack and improve the operating efficiency.

[0045] Further, the waste liquid collection chamber and the disinfection chamber are connected to the collection box 13 through the three-way pipeline 12 and the pump body 11, realizing the automatic management of waste liquid and disinfectant. This design reduces manual operation, improves work efficiency, and ensures the safe treatment of waste liquid and disinfectant. After the cover plate 25 above the disinfection chamber is opened, the mechanical arm 3 can be used to clamp the contaminated tools such as test tubes and pipettes, and immerse them in the disinfection machine in the disinfection chamber for disinfection treatment. The waste liquid collection chamber is connected to the waste liquid collection tank 24, which is used to collect the waste liquid flowing inside the waste liquid collection tank 24. The waste liquid can be the waste liquid generated during the proliferation or purification process, and can also be used to collect the disinfectant sprayed by the subsequent disinfection pipeline 14. The pipeline 12 and the pump body 11 are used to extract the waste liquid and pump it into the collection box 13 for its subsequent treatment. The disinfectant agent in the other collection box 13 re-injects the disinfectant into the disinfection chamber to achieve subsequent disinfection treatment.

[0046] Furthermore, the hidden protector 4 safely encapsulates the proliferation device 5 and the purification device 5A in the hidden cover 41 through the design of the hidden groove 42, the carrier encapsulation plate 43 and the driving component 44. The carrier encapsulation plate 43 is controlled to open and close by the driving component 44, which is convenient for the maintenance and replacement of the equipment, while keeping the operating space clean and closed, reducing the risk of contamination.

[0047] Furthermore, the proliferation equipment 5 and the purification equipment 5A are respectively some types of equipment suitable for the proliferation and purification of porcine circovirus, such as a proliferation dish, a preliminary centrifuge, a high-speed centrifuge and a chromatography purifier.

[0048] Here’s how it works:

[0049] First, the system initialization and preparation phase:

[0050] System startup: The operator starts the system, the integrated operating table 2 starts to supply power, and components such as the linear actuator 1, the temperature control device 10, the disinfection pipeline 14 and the disinfection lamp 15 enter the standby state.

[0051] Environmental preparation: The temperature control device 10 starts to adjust the temperature in the operating space according to the preset parameters to ensure the best environmental conditions for virus proliferation and purification. At the same time, the disinfection lamp 15 in the disinfection pipeline 14 is turned on to perform preliminary disinfection of the operating space to kill potential bacteria and viruses.

[0052] Equipment inspection: The robot arm 3 performs self-inspection through its flexible gripper to ensure that the gripper is flexible and can accurately grasp the tool. At the same time, check whether the proliferation device 5 and the purification device 5A are in normal working condition.

[0053] Then, the virus proliferation stage:

[0054] Tool grabbing: According to the preset program, the robotic arm 3 uses flexible grippers to accurately grab the tools required in the proliferation process, such as test tubes, pipettes, etc., and moves to the top of the proliferation device 5.

[0055] Proliferation operation: The robot arm 3 delivers the tool into the proliferation device 5 to perform virus inoculation, culture and other proliferation operations. During this process, the operator can observe the operation through the isolation transparent plate 8, but the isolation transparent plate remains closed to prevent external contamination.

[0056] Environmental maintenance: The temperature control device 10 continuously monitors and adjusts the temperature, and the disinfection pipeline 14 and the disinfection lamp 15 are started regularly to disinfect the operation space to ensure the sterility of the proliferation environment.

[0057] Next, the virus purification stage:

[0058] Tool transfer: After the proliferation is completed, the robot arm 3 starts again to transfer the propagated virus sample and the required tools from the proliferation device 5 to the top of the purification device 5A.

[0059] Purification operation: The robot arm 3 sends the tool into the purification device 5A to perform virus purification operations, such as centrifugation, filtration and other steps, to remove impurities and improve the purity of the virus.

[0060] Waste liquid treatment: The waste liquid generated during the purification process is collected through the waste liquid collection chamber and automatically discharged into the collection box 13 through the three-way pipeline 12 and the pump body 11 to achieve centralized treatment of the waste liquid.

[0061] Finally, the finishing and cleaning phase:

[0062] Tool return: The robot arm 3 puts the used tools back into the tool placement cavity and accurately fixes items such as test tube racks through the positioning seat 21.

[0063] Comprehensive disinfection: The disinfection pipeline 14 and the disinfection lamp 15 are started again to comprehensively disinfect the operating space to ensure that no virus remains.

[0064] System shutdown: The operator checks the status of each system component and turns off the system power after confirming that everything is correct, thus ending the proliferation and purification process.

[0065] Through the above detailed workflow, the efficient PCV2 virus proliferation and purification system provided by the present invention can automatically and efficiently complete the virus proliferation and purification tasks while ensuring the safety and cleanliness of the operating environment.

[0066] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An efficient PCV2 virus proliferation and purification system, characterized by: The invention comprises a linear actuator (1), an integrated operating table (2), a mechanical arm (3), a hidden protector (4), a proliferation device (5) and a purification device (5A), wherein the linear actuator (1) is fixedly mounted on a bearing platform (21) of the integrated operating table (2), a mechanical arm (3) is fixedly mounted on a sliding platform of the linear actuator (1), the mechanical arm (3) is located between two hidden machine covers (41) of the hidden protectors (4), the hidden machine covers (41) of the two hidden protectors (4) are fixedly mounted on the bearing platform (21) of the integrated operating table (2) in a mirror-symmetrical manner, and the proliferation device (5) or the purification device (5A) is fixedly mounted in the two hidden protectors (4) respectively; A flexible clamp is fixedly mounted on the output end of the mechanical arm (3) and is used to grab tools used in the proliferation or purification process.

2. The highly efficient PCV2 virus proliferation and purification system according to claim 1, characterized in that: A guide rail frame (6) is fixedly mounted on the bearing platform (21), a storage cover (7) is fixedly mounted on the guide rail frame (6), an isolation transparent plate (8) is slidably mounted inside the guide rail frame (6) and the storage cover (7), and an operation panel (9) is fixedly mounted on the outer side of the guide rail frame (6).

3. A highly efficient PCV2 virus proliferation and purification system as claimed in claim 2, characterized in that: The insulating transparent plate (8) is provided with a raised handle and a sealing groove (23) is provided below the insulating transparent plate. The sealing groove (23) is provided on the bearing platform (21).

4. A highly efficient PCV2 virus proliferation and purification system as claimed in claim 3, characterized in that: A back plate (16) is fixedly mounted on the carrying platform (21), and a temperature control device (10) is fixedly mounted on the back plate (16) and the two hidden protectors (4), and the temperature control device (10) is used to control the temperature of the sealed space below.

5. The highly efficient PCV2 virus proliferation and purification system according to claim 4, characterized in that: A disinfection pipeline (14) is fixedly installed on the back plate (16), the two hidden protectors (4) and the guide rail frame (6); the disinfection pipeline (14) is located below the temperature control device (10); a disinfection lamp (15) is fixedly installed inside the disinfection pipeline (14); and the disinfection lamp (15) is used to disinfect and sterilize the space below.

6. A highly efficient PCV2 virus proliferation and purification system as claimed in claim 5, characterized in that: The disinfection pipeline (14) and the disinfection lamp (15) are not arranged at the exhaust port of the temperature control device (10), and thus do not interfere with the direction of the airflow discharged from the temperature control device (10).

7. A highly efficient PCV2 virus proliferation and purification system as claimed in claim 6, characterized in that: A plurality of disinfection spray heads are arranged below the disinfection pipeline (14), and the disinfection pipeline (14) is a pipe structure with a square outside and a round inside.

8. The highly efficient PCV2 virus proliferation and purification system according to claim 1, characterized in that: The integrated operating table (2) comprises a transverse slide groove (22), a waste liquid collection groove (24), a cover plate (25), and an integrated box (26); the transverse slide groove (22) and the waste liquid collection groove (24) are arranged on the surface of a bearing platform (21), wherein a linear actuator (1) is fixedly installed inside the transverse slide groove (22); a cover plate (25) is embedded in the opening of the waste liquid collection groove (24), and the cover plate (25) and the waste liquid collection groove (24) together form a waste liquid collection channel; and a bearing platform (21) is fixedly installed above the integrated box (26).

9. A highly efficient PCV2 virus proliferation and purification system as claimed in claim 8, characterized in that: A plurality of cavities (28) are arranged in the integrated box (26), wherein work tables (29) are arranged at the openings of two of the cavities (28), and the work tables (29) are rotatably mounted on the inner side of the bearing platform (21). The output end of the servo motor (20) fixedly mounted on the bearing platform (21) is fixed to one end of the corresponding work table (29), and the servo motor (20) is used to control the opening and closing of the work table (29).

10. The highly efficient PCV2 virus proliferation and purification system according to claim 9, characterized in that: The cavity (28) is divided into a waste liquid collection cavity, a tool placement cavity and a disinfection cavity. Tools required for virus proliferation or purification are placed inside the tool placement cavity. Workbenches (29) are arranged at the openings of the tool placement cavity and the disinfection cavity. A positioning seat (21) is fixedly installed above one of the workbenches (29). The positioning seat (21) is used to position a test tube rack.

11. A highly efficient PCV2 virus proliferation and purification system as claimed in claim 10, characterized in that: The waste liquid collection chamber and the disinfection chamber of the cavity (28) are connected to a collection box (13) storing waste liquid internally through a three-way pipeline (12) and a pump body (11), and the collection box (13) storing disinfectant internally is connected to the disinfection chamber of the cavity (28) through a pipeline and a pump body (11).

12. The highly efficient PCV2 virus proliferation and purification system according to claim 1, characterized in that: The hidden protector (4) further comprises a hidden slot (42), a carrier packaging plate (43) and a driving component (44); a plurality of hidden slots (42) are arranged in the hidden hood (41); a carrier packaging plate (43) is rotatably mounted at the opening of the hidden slot (42); each of the carrier packaging plates (43) is fixed to an output end of a corresponding driving component (44); and the driving component (44) is fixedly mounted on the outside of the hidden hood (41).

13. A highly efficient PCV2 virus proliferation and purification system as claimed in claim 12, characterized in that: A proliferation device (5) and a purification device (5A) are fixedly installed on the inner side of each carrier packaging plate (43), and the proliferation device (5) and the purification device (5A) are arranged in the hidden groove (42).

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