Device for fermenting gas-consuming microorganisms and absorbing and utilizing carbon dioxide

Abstract

The invention relates to a device for fermenting by utilizing gas-feeding microorganisms and absorbing and utilizing carbon dioxide, which is characterized in that a stirring shaft with an air passage is arranged in each fermentation tank, an aeration stirring paddle is arranged on a first stirring shaft, a gas storage tank pipeline is communicated with the upper end of the first air passage, and the first air passage is communicated with an inner cavity of the aeration stirring paddle; the first fermentation tank is communicated with the upper end of a second air channel through a pipeline, the lower end of the second air channel is communicated with an inner cavity of the air spraying pipe A. The lower end of a third stirring shaft is provided with an air spraying pipe B. The second fermentation tank is communicated with the upper end of a third air channel through a pipeline, and the lower end of the third air channel is communicated with an inner cavity of the air spraying pipe B. The third fermentation tank is communicated with the upper end of a fourth air channel through a pipeline, the lower end of the fourth air channel is communicated with an inner cavity of the aeration pipe, all the fermentation tanks are sequentially connected through fermentation liquid pipelines, and the volumes of the fermentation tanks are sequentially halved. According to the invention, the absorption and utilization rate of CO2 can be increased to more than 90% at one time.

Description

Technical Field

[0001] The invention relates to the technical field of microbial fermentation, in particular to a device for utilizing gas-eating microorganisms for fermentation and absorbing and utilizing carbon dioxide. Background Art

[0002] With the development of social economy, the concentration of carbon dioxide in the atmosphere continues to rise, and the large-scale removal and comprehensive utilization of carbon dioxide has become one of the important issues that cannot be avoided in the field of energy decision-making and energy management.

[0003] In the prior art, chemical catalytic methods (such as the Fischer-Tropsch synthesis) can be used to convert gases containing carbon dioxide, carbon monoxide and / or hydrogen, such as industrial waste gas or synthesis gas, into various fuels and chemicals. With the development of technology, microbial gas fermentation methods that can biologically fix the above gases have gradually been promoted and applied. Compared with chemical catalytic methods, microbial gas fermentation methods have mild process conditions, low energy consumption, high product specificity, high yield and stronger toxicity resistance, and therefore have good development prospects.

[0004] In the field of biotechnology, fermentation by aerobic microorganisms is considered a promising method for CO2 absorption and utilization. Aerobic microorganisms are a major type of chemo-carbon-fixing bacteria, belonging to the gram-positive anaerobic Clostridium. Currently known aerobic microorganisms include Clostridium ljungdahlii, Clostridium autoethanogenum, Clostridium carboxidivorans, and Acetobacterium woodii. In addition to fixing C1 gases such as CO / CO2, aerobic microorganisms can also be converted into an important metabolic intermediate, acetyl-CoA, after a series of catalytic reactions, and then synthesized into various products through various branch metabolic pathways, such as acetic acid, ethanol, butanol, hexanol and other chemicals and fuels, so it has a good prospect for industrial application.

[0005] However, most of the tanks used for gas-eating microbial fermentation in the prior art are single-tank fermentation systems, and their CO2 absorption and utilization rate is only 50-60%, which means that a large amount of CO2 cannot be effectively absorbed, which not only causes waste of raw gas, but also increases the cost and complexity of exhaust gas treatment.

[0006] Patent CN112300971B discloses a method for improving carbon monoxide utilization rate of Clostridium aerovorum by high-throughput gene synthesis, but the carbon monoxide utilization rate of the engineered Clostridium aerovorum in this method is ≥30.0%, preferably ≥37.5%, and more preferably ≥40.0%.

[0007] Patent CN105039416B discloses a method for improving the fermentation efficiency of anaerobic aerobic microorganisms. The fermentation system is still a single-tank system, but the method uses a hollow cellulose membrane to filter and retain the bacterial liquid of the anaerobic aerobic microorganisms in the fermentation system, and then back-washes it with a culture medium and ferments the retained bacteria again, thereby achieving multiple continuous fermentation effects. However, it can be seen from the embodiments of the patent that the production of alcohol substances such as ethanol by this method needs to be further improved. Summary of the invention

[0008] The purpose of the present invention is to provide a device for utilizing gas-eating microorganisms for fermentation and absorption and utilization of carbon dioxide. The device adopts a plurality of fermentation tanks with volumes halved successively in series to realize multi-stage utilization of CO2. At the same time, different types of stirring shaft structures are designed for tanks of different volumes. The absorption and utilization rate of CO2 can be increased to more than 90% at one time, and the output of alcohol products is also greatly improved.

[0009] The objective of the present invention is achieved through the following technical solutions:

[0010] A device for utilizing gas-eating microorganisms for fermentation and absorbing and utilizing carbon dioxide, comprising a first fermentation tank, a second fermentation tank, a third fermentation tank, a fourth fermentation tank and a gas storage tank, wherein a first stirring shaft is provided inside the first fermentation tank, and an aeration stirring paddle with aeration holes of paddle blades is provided on the first stirring shaft, a first airway is provided inside the first stirring shaft, and the gas storage tank is connected with the upper end of the first airway through an air inlet pipeline, a connecting hole connecting the first airway and the inner cavity of the aeration stirring paddle is provided on the shaft wall of the first stirring shaft, a second stirring shaft is provided inside the second fermentation tank, and an injection pipe A with multiple gas nozzle assemblies is provided at the lower end of the second stirring shaft, a second airway is provided inside the second stirring shaft, and the upper end of the first fermentation tank is connected with the upper end of the second airway through a first air outlet pipeline, and the lower end of the second airway is connected with the inner cavity of the injection pipe A, a third stirring shaft is provided inside the third fermentation tank A shaft is provided, and an air jet pipe B with a plurality of air jet nozzles is provided at the lower end of the third stirring shaft, a third air channel is provided inside the third stirring shaft, and the upper end of the second fermentation tank is communicated with the upper end of the third air channel through the second air outlet pipeline, and the lower end of the third air channel is communicated with the inner cavity of the air jet pipe B, a honeycomb guide cylinder is provided on the third stirring shaft, a fourth stirring shaft is provided inside the fourth fermentation tank, and an aeration pipe with aeration holes is provided at the lower end of the fourth stirring shaft, a fourth air channel is provided inside the fourth stirring shaft, and the upper end of the third fermentation tank is communicated with the upper end of the fourth air channel through the third air outlet pipeline, and the lower end of the fourth air channel is communicated with the inner cavity of the aeration pipe, the first fermentation tank, the second fermentation tank, the third fermentation tank and the fourth fermentation tank are connected in sequence through corresponding fermentation liquid pipelines and their volumes are halved in sequence, and fermentation liquid feeding pipes are provided at the upper ends of the first fermentation tank, the second fermentation tank, the third fermentation tank and the fourth fermentation tank.

[0011] The gas nozzle assembly includes a reducing nozzle, a connecting frame and a spray rod, wherein the spray rod is provided with a suction nozzle at one end and an output nozzle at the other end, the reducing nozzle is installed on the injection pipe A, and the reducing nozzle and the suction nozzle are respectively arranged on both sides of the connecting frame.

[0012] A first gas outlet is provided at the upper end of the first fermentation tank, and the first gas outlet is connected to the upper end of the second gas channel through a first gas outlet pipeline; a second gas outlet is provided at the upper end of the second fermentation tank, and the second gas outlet is connected to the upper end of the third gas channel through a second gas outlet pipeline; a third gas outlet is provided at the upper end of the third fermentation tank, and the third gas outlet is connected to the upper end of the fourth gas channel through a third gas outlet pipeline; a fourth gas outlet is provided at the upper end of the fourth fermentation tank, and the fourth gas outlet is connected to a gas output pipeline.

[0013] An air intake flow meter is provided on the air intake pipeline, a gas output flow meter is provided on the gas output pipeline, and an air outlet flow control valve is provided on the first air outlet pipeline, the second air outlet pipeline, and the third air outlet pipeline.

[0014] A feeding port is provided at the upper end of the first fermentation tank, a first liquid inlet is provided at one side of the first fermentation tank, a second liquid outlet is provided at one side of the second fermentation tank and a second liquid inlet is provided at the other side, a third liquid outlet is provided at one side of the third fermentation tank and a third liquid inlet is provided at the other side, a fourth liquid outlet is provided at one side of the fourth fermentation tank, wherein the first liquid inlet is connected to the second liquid outlet through a first fermentation liquid pipeline, the second liquid inlet is connected to the third liquid outlet through a second fermentation liquid pipeline, and the third liquid inlet is connected to the fourth liquid outlet through a third fermentation liquid pipeline, and a discharge port is provided at the lower end of the first fermentation tank, the lower end of the second fermentation tank, the lower end of the third fermentation tank and the lower end of the fourth fermentation tank.

[0015] The first fermentation liquid pipeline, the second fermentation liquid pipeline and the third fermentation liquid pipeline are all provided with a fermentation liquid flow meter and a fermentation liquid pipeline control valve.

[0016] A first feed port is provided at the upper end of the first fermentation tank, a second feed port is provided at the upper end of the second fermentation tank, a third feed port is provided at the upper end of the third fermentation tank, and a fourth feed port is provided at the upper end of the fourth fermentation tank. The first feed port, the second feed port, the third feed port and the fourth feed port are respectively connected to a feed main pipe through corresponding fermentation liquid feed pipes, the feed main pipe is connected to a feed tank, and the fermentation liquid feed pipe is provided with a feed flow control valve.

[0017] The first fermentation tank has a first drive assembly at the upper end for driving the first stirring shaft to rotate, the second fermentation tank has a second drive assembly at the upper end for driving the second stirring shaft to rotate, the third fermentation tank has a third drive assembly at the upper end for driving the third stirring shaft to rotate, and the fourth fermentation tank has a fourth drive assembly at the upper end for driving the fourth stirring shaft to rotate.

[0018] The first drive assembly, the second drive assembly, the third drive assembly and the fourth drive assembly have the same structure, and all include a stirring reduction motor, a transmission belt and a stirring shaft adapter, wherein a rotating shaft sleeve is provided inside the stirring shaft adapter and is sleeved on the corresponding pipeline, a driven pulley is sleeved on the outside of the stirring shaft adapter, a driving pulley is provided on the output shaft of the stirring reduction motor, and the driving pulley is connected to the driven pulley through a transmission belt.

[0019] The first fermentation tank, the second fermentation tank, the third fermentation tank and the fourth fermentation tank are all provided with sensor installation ports and sampling ports; the first stirring shaft is also provided with a first defoaming paddle, the second stirring shaft is also provided with a second defoaming paddle and a stirring blade A, the third stirring shaft is also provided with a third defoaming paddle, and the third defoaming paddle is arranged on the upper side of the honeycomb guide tube, and the fourth stirring shaft is also provided with a fourth defoaming paddle and a stirring blade B.

[0020] The advantages and positive effects of the present invention are:

[0021] 1. The present invention uses a plurality of fermentation tanks with volumes halved in sequence to achieve multi-stage utilization of CO2. Different types of stirring shaft structures are designed according to tanks of different volumes, which can increase the absorption and utilization rate of CO2 to more than 90% at one time, and the output of alcohol products is also greatly improved.

[0022] 2. In the first fermentation tank with the largest volume, the aeration stirring paddle on the first stirring shaft performs aeration through the paddle aeration holes, and the bubbles can be dispersed by the paddles to achieve a more dispersed gas distribution.

[0023] 3. In the second fermentation tank of the present invention, the gas nozzle assembly on the jet pipe A includes a reduced diameter nozzle, a connecting frame and a spray rod, wherein the gas is first sprayed from the reduced diameter nozzle to the hollow part of the connecting frame, at which time its static pressure energy is converted into velocity energy and the pressure of the suction nozzle is also reduced, and under the spraying action of the gas, the fermentation liquid enters the spray rod together with the gas and is sprayed out through the output nozzle, so that the gas and liquid phases in the spray rod develop from preliminary mixing to intense mixing oscillation, and generate gas dynamic foaming and pressure foaming, so that the fermentation liquid and air are crushed into a large number of tiny particle groups, creating a highly turbulent, expanded and continuously renewed emulsified interface between gas and liquid, and at the same time can improve the gas solubility, and significantly reduce the stirring power required by the stirring paddle, reduce the shear force damage of the stirring paddle to the fermentation bacterial liquid, which can meet the gas distribution needs of the second fermentation tank with a volume reduced by half.

[0024] 4. In the third fermentation tank of the present invention, taking into account the characteristics of the countercurrent flow of the fermentation liquid and the gas, a honeycomb guide tube is arranged on the third stirring shaft, which not only increases the solid-liquid contact area but also has a guiding effect and is beneficial to the mass transfer and gas distribution of the fermentation medium. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a structural schematic diagram of the present invention,

[0026] Figure 2 for Figure 1 The structural diagram of the first fermentation tank.

[0027] Figure 3 for Figure 2 A in the enlarged view,

[0028] Figure 4 for Figure 2 The enlarged view of point B in the figure,

[0029] Figure 5 for Figure 1 The structural diagram of the second fermentation tank,

[0030] Figure 6 for Figure 5 Schematic diagram of the structure of the gas nozzle assembly.

[0031] Figure 7 for Figure 1 The structural diagram of the third fermentation tank.

[0032] Figure 8 for Figure 1 The structural diagram of the fourth fermentation tank,

[0033] Fig. 9 The figure is a schematic diagram of the effect of an application example of the present invention.

[0034] Among them, 1 is the first fermentation tank, 101 is the stirring reduction motor, 102 is the transmission belt, 103 is the first stirring shaft, 1031 is the first defoaming paddle, 1032 is the aeration stirring paddle, 10321 is the paddle aeration hole, 10322 is the connecting channel, 1033 is the stirring shaft adapter, 10331 is the rotating shaft sleeve, 10332 is the driven pulley, 1034 is the first air channel, 104 is the first air outlet, 105 is the first feeding port, 106 is the first liquid inlet, 107 is the sensor installation port, 108 is the take-out port, The sample port is 109, the material outlet is 110, the material feeding port is 2, the second fermentation tank is 201, the second liquid outlet is 202, the second liquid inlet is 203, the second stirring shaft is 2031, the second defoaming paddle is 2032, the stirring blade A is 2033, the second air channel is 204, the second gas outlet is 205, the second feeding port is 206, the second driving assembly is 207, the jet pipe A is 208, the gas nozzle assembly is 2081, the reduced diameter nozzle is 2082, the suction nozzle is 2083, the spray rod is 2084, and the output nozzle is nozzle, 2085 is a connecting frame, 3 is a third fermentation tank, 301 is a third liquid outlet, 302 is a second liquid inlet, 303 is a third stirring shaft, 304 is a third gas outlet, 305 is a third feed port, 306 is a third driving assembly, 307 is an air jet pipe B, 308 is an air jet nozzle, 309 is a honeycomb guide tube, 4 is a fourth fermentation tank, 401 is a fourth liquid outlet, 402 is a gas output flow meter, 403 is a fourth stirring shaft, 4031 is a fourth defoaming paddle, 4032 is a stirring blade B, 4033 is an aeration air pipe, 404 is the fourth air outlet, 405 is the fourth feed port, 406 is the fourth drive assembly, 5 is a gas storage tank, 6 is a feed tank, 7 is a feed main pipe, 701 is a fermentation liquid feed pipe, 8 is an air inlet pipeline, 801 is an air inlet flow meter, 9 is a first air outlet pipeline, 10 is a second air outlet pipeline, 11 is a third air outlet pipeline, 12 is an air outlet flow control valve, 13 is a first fermentation liquid pipeline, 14 is a second fermentation liquid pipeline, 15 is a third fermentation liquid pipeline, 16 is a fermentation liquid flow meter, and 17 is a fermentation liquid pipeline control valve. DETAILED DESCRIPTION

[0035] The present invention will be further described below in conjunction with the accompanying drawings.

[0036] like Figures 1 to 9 As shown, the present invention comprises a first fermentation tank 1, a second fermentation tank 2, a third fermentation tank 3, a fourth fermentation tank 4 and a gas storage tank 5, wherein as Figures 1 to 3As shown, a first stirring shaft 103 is provided inside the first fermentation tank 1, and an aeration stirring paddle 1032 with a blade aeration hole 10321 is provided on the first stirring shaft 103, a first air channel 1034 is provided inside the first stirring shaft 103, and the gas storage tank 5 is connected to the upper end of the first air channel 1034 through the air inlet pipeline 8, and a connecting channel 10322 connecting the first air channel 1034 and the inner cavity of the aeration stirring paddle 1032 is provided on the shaft wall of the first stirring shaft 103, as shown in FIG. Figure 5 As shown, the second fermentation tank 2 is provided with a second stirring shaft 203 inside, and the lower end of the second stirring shaft 203 is provided with an air injection pipe A207 with multiple gas nozzle assemblies 208, the second stirring shaft 203 is provided with a second air channel 2033 inside, and the upper end of the first fermentation tank 1 is connected to the upper end of the second air channel 2033 through the first air outlet pipeline 9, and the lower end of the second air channel 2033 is connected to the inner cavity of the air injection pipe A207, as shown in FIG. Figure 7 As shown, the third fermentation tank 3 is provided with a third stirring shaft 303 inside, and the lower end of the third stirring shaft 303 is provided with an air jet pipe B307 with multiple air jet nozzles 308, the third stirring shaft 303 is provided with a third air channel inside, and the upper end of the second fermentation tank 2 is connected to the upper end of the third air channel through the second air outlet pipeline 10, and the lower end of the third air channel is connected to the inner cavity of the air jet pipe B307, and the third stirring shaft 303 is provided with a honeycomb guide tube 309, as shown in FIG. Figure 8 As shown, the fourth fermentation tank 4 is provided with a fourth stirring shaft 403, and the lower end of the fourth stirring shaft 403 is provided with an aeration pipe 4033 with aeration holes, the fourth stirring shaft 403 is provided with a fourth airway, and the upper end of the third fermentation tank 3 is connected to the upper end of the fourth airway through the third air outlet pipeline 11, and the lower end of the fourth airway is connected to the inner cavity of the aeration pipe 4033. Figure 1 As shown, the first fermentation tank 1, the second fermentation tank 2, the third fermentation tank 3 and the fourth fermentation tank 4 are connected in sequence through corresponding fermentation liquid pipelines and their volumes are halved in sequence, and the upper ends of the first fermentation tank 1, the second fermentation tank 2, the third fermentation tank 3 and the fourth fermentation tank 4 are all provided with fermentation liquid feeding pipes 701.

[0037] like Figures 1 to 9As shown, in this embodiment, a first gas outlet 104 is provided at the upper end of the first fermentation tank 1, and the first gas outlet 104 is connected to the upper end of the second gas channel 2033 through a first gas outlet pipeline 9, a second gas outlet 204 is provided at the upper end of the second fermentation tank 2, and the second gas outlet 204 is connected to the upper end of the third gas channel through a second gas outlet pipeline 10, a third gas outlet 304 is provided at the upper end of the third fermentation tank 3, and the third gas outlet 304 is connected to the upper end of the fourth gas channel through a third gas outlet pipeline 11, a fourth gas outlet 404 is provided at the upper end of the fourth fermentation tank 4, and the fourth gas outlet 404 is connected to a gas output pipeline. When the present invention is working, CO2 in the gas storage tank 5 is input into the first air channel 1034 in the first stirring shaft 103 in the first fermentation tank 1 through the air inlet pipeline 8, and is output into the tank body of the first fermentation tank 1 through the paddle liquid aeration holes 10321 on each aeration stirring paddle 1032 during the rotation of the first stirring shaft 103, and then the fermentation gas in the first fermentation tank 1 is output from the first air outlet 104 and enters the second air channel 203 in the second stirring shaft 203 through the first air outlet pipeline 9, and is ejected into the tank body of the second fermentation tank 2 through the jet pipe A207 at the lower end thereof during the rotation of the second stirring shaft 203, and then the second fermentation tank 2 is The fermentation gas in tank 2 is output from the second gas outlet 204 and enters the third air channel in the third stirring shaft 303 through the second gas outlet pipeline 10, and then is ejected into the tank body of the third fermentation tank 3 through the jet pipe B307 at the lower end of the third stirring shaft 303. Then, the fermentation gas in the third fermentation tank 3 is output from the third gas outlet 304 and enters the fourth air channel in the fourth stirring shaft 403 through the third gas outlet pipeline 11, and is output into the tank body of the fourth fermentation tank 4 through the aeration pipe 4033 at the lower end of the fourth stirring shaft 403. Finally, the excess gas in the fourth fermentation tank 4 can enter the gas output pipeline through the fourth gas outlet 404 and be discharged.

[0038] First, one of the purposes of the present invention is to solve the problem of low CO2 absorption utilization rate (60% in this embodiment) in the existing single fermentation tank system. Assuming that CO2 intake 1m 3 If the exhaust gas is 0.4m 3 The present invention realizes multi-stage utilization of CO2 by connecting multiple fermentation tanks in series, and the volume of each fermentation tank is halved successively, so that its theoretical utilization efficiency is as follows:

[0039]

[0040] Secondly, the present invention designs different types of stirring shaft structures according to different volume tank conditions. In the above-mentioned gas flow process, in the first fermentation tank 1 with the largest volume, the aeration stirring paddle 1032 on the first stirring shaft 103 is aerated through the paddle aeration hole 10321, and the bubbles can be dispersed by the paddle to achieve a more dispersed gas distribution. In the second fermentation tank 2, the gas nozzle assembly 208 on the jet pipe A207 is as shown in FIG. Figure 6 As shown, it includes a reduced diameter nozzle 2081, a connecting frame 2085 and a spray rod 2083, wherein the spray rod 2083 is provided with a suction nozzle 2082 at one end and an output nozzle 2084 at the other end, the reduced diameter nozzle 2081 is installed on the injection pipe A207, and the reduced diameter nozzle 2081 and the suction nozzle 2082 are respectively arranged on both sides of the connecting frame 2085, and the gas is first sprayed from the reduced diameter nozzle 2081 to the hollow part of the connecting frame 2085, at which time its static pressure energy is converted into velocity energy, and the pressure of the suction nozzle 2082 is also reduced. Under the spraying action of the gas, the fermentation liquid enters the spray rod 2083 together with the gas, and is sprayed out through the output nozzle 2084, so that the gas and liquid phases in the spray rod 2083 are initially mixed and developed. It is a violent mixing and oscillation, and produces gas dynamic foaming and pressure foaming, so that the fermentation liquid and air are crushed into a large number of tiny particle groups, creating a highly turbulent, expanding and constantly renewed emulsified interface between gas and liquid, and at the same time can improve the gas solubility, and significantly reduce the stirring power required by the stirring paddle, and reduce the shear force damage of the stirring paddle to the fermentation liquid. In the third fermentation tank 3, since the flow direction of the fermentation liquid is opposite to that of the gas, the honeycomb guide tube 309 on the third stirring shaft 303 increases the solid-liquid contact area and has a guiding effect, which is beneficial to the mass transfer and gas distribution of the fermentation medium. Finally, in the fourth fermentation tank 4, the aeration pipe 4033 at the lower end of the fourth stirring shaft 403 can make full use of the residual fermentation gas, which can further improve the gas utilization rate. The present invention can ensure the CO2 absorption utilization rate of the actual equipment system through the above-mentioned structural combination design, such as Fig. 9 As shown, in one application example of the present invention, the absorption utilization rate of CO2 can be increased to more than 90% at one time, and the acetic acid output is also greatly improved.

[0041] like Figure 1 and Figure 8 As shown, in this embodiment, the air intake pipeline 8 is provided with an air intake flow meter 801, and the gas output pipeline is provided with a gas output flow meter 402 for real-time monitoring of the air intake and output volume of the present invention. Figure 1 As shown, in this embodiment, the first gas outlet pipeline 9, the second gas outlet pipeline 10, and the third gas outlet pipeline 11 are all provided with gas outlet flow control valves 12 to control the gas outlet flow of each fermentation tank.

[0042] like Figures 1 to 9 As shown, in this embodiment, a feeding port 101 is provided at the upper end of the first fermentation tank 1, a first liquid inlet 106 is provided at one side of the first fermentation tank 1, a second liquid outlet 201 is provided at one side of the second fermentation tank 2, and a second liquid inlet 202 is provided at the other side, a third liquid outlet 301 is provided at one side of the third fermentation tank 3, and a third liquid inlet 302 is provided at the other side, and a fourth liquid outlet 401 is provided at one side of the fourth fermentation tank 4, wherein the first liquid inlet 106 is connected to the second liquid outlet 201 through a first fermentation liquid pipeline 13, the second liquid inlet 202 is connected to the third liquid outlet 301 through a second fermentation liquid pipeline 14, and the third liquid inlet 302 is connected to the fourth liquid outlet 401 through a third fermentation liquid pipeline 15, and a discharge port 109 is provided at the lower end of the first fermentation tank 1, the lower end of the second fermentation tank 2, the lower end of the third fermentation tank 3 and the lower end of the fourth fermentation tank 4. When the present invention is working, the feeding port 101 is used to feed a certain amount of fermentation liquid into the first fermentation tank 1. At the same time, the flow direction of the fermentation liquid in each tank is opposite to that of the gas, that is, the fermentation liquid flows along the fourth fermentation tank 4, the third fermentation tank 3, and the second fermentation tank 2, and is finally enriched in the first fermentation tank 1. Figures 1 to 9 As shown, the first fermentation tank 1 is provided with a first feed port 105 at the upper end, the second fermentation tank 2 is provided with a second feed port 205 at the upper end, the third fermentation tank 3 is provided with a third feed port 305 at the upper end, and the fourth fermentation tank 4 is provided with a fourth feed port 405 at the upper end. The first feed port 105, the second feed port 205, the third feed port 305 and the fourth feed port 405 are respectively connected to a feed main pipe 7 through corresponding fermentation liquid feed pipes 701, and the feed main pipe 7 is connected to a feed tank 6. The above structure is used to supplement fresh culture medium into each tank body, and the fermentation liquid feed pipe 701 is provided with a feed flow control valve to control the feed amount.

[0043] In addition, Figure 1 As shown, in this embodiment, the first fermentation liquid pipeline 13, the second fermentation liquid pipeline 14, and the third fermentation liquid pipeline 15 are all provided with a fermentation liquid flow meter 16 and a fermentation liquid pipeline control valve 17 to control the fermentation liquid flow and pipeline opening and closing.

[0044] like Figures 1 to 9As shown, the first fermentation tank 1 is provided with a first driving assembly at the upper end thereof for driving the first stirring shaft 103 to rotate, the second fermentation tank 2 is provided with a second driving assembly 206 at the upper end thereof for driving the second stirring shaft 203 to rotate, the third fermentation tank 3 is provided with a third driving assembly 306 at the upper end thereof for driving the third stirring shaft 303 to rotate, and the fourth fermentation tank 4 is provided with a fourth driving assembly 406 at the upper end thereof for driving the fourth stirring shaft 403 to rotate. In this embodiment, the first driving assembly, the second driving assembly 206, the third driving assembly 306 and the fourth driving assembly 406 have the same structure and all include a stirring reduction motor 101, a transmission belt 102 and a stirring shaft adapter 1033, wherein the first driving assembly 206, the second driving assembly 206 and the third driving assembly 306 and the fourth driving assembly 406 are provided with ... Figure 3 Taking the first driving component shown as an example, the stirring shaft adapter 1033 is arranged at the upper end of the first stirring shaft 103, and a rotating sleeve 10331 is provided inside the stirring shaft adapter 1033 and is sleeved on the output end of the intake pipe 8, so that the stirring shaft adapter 1033 is rotationally connected with the intake pipe 8, and at the same time, the intake pipe 8 is connected to the first air duct 1034 inside the first stirring shaft 103, and a driven pulley 10322 is sleeved on the outer side of the stirring shaft adapter 1033, and a driving pulley is provided on the output shaft of the stirring reduction motor 101, and the driving pulley is connected to the driven pulley 10322 through a transmission belt 102, and the stirring shaft adapters of the remaining driving components are replaced with rotationally connected with the corresponding output ends of the outlet pipe. Figure 2 As shown, each fermentation tank body is provided with a bearing at a position where the stirring shaft passes through to support its rotation.

[0045] like Figure 2 As shown, the first fermentation tank 1, the second fermentation tank 2, the third fermentation tank 3, and the fourth fermentation tank 4 are all provided with a sensor installation port 107 and a sampling port 108. The sensor installation port 107 can be used to install appropriate sensors according to actual needs, such as temperature sensors, pressure sensors, etc., and the sampling port 108 is used for sampling and analysis.

[0046] like Figure 2 As shown, in this embodiment, the first stirring shaft 103 is further provided with a first defoaming paddle 1031. Figure 5 As shown, in this embodiment, the second stirring shaft 203 is further provided with a second defoaming paddle 2031 and a stirring paddle A2032. Figure 7 As shown, in this embodiment, the third stirring shaft 303 is further provided with a third defoaming paddle, and the third defoaming paddle is arranged on the upper side of the honeycomb guide tube 309, as shown in FIG. Figure 8 As shown, in this embodiment, the fourth stirring shaft 403 is further provided with a fourth defoaming paddle 4031 and a stirring blade B4032.

[0047] The working principle of the present invention is:

[0048] When the present invention is working, CO2 in the gas storage tank 5 is input into the first air channel 1034 in the first stirring shaft 103 through the air inlet pipeline 8, and is output into the tank body of the first fermentation tank 1 through the paddle liquid aeration holes 10321 on each aeration stirring paddle 1032 during the rotation of the first stirring shaft 103, and then the fermentation gas in the first fermentation tank 1 is output from the first air outlet 104 and enters the second air channel 203 in the second stirring shaft 203 through the first air outlet pipeline 9, and is ejected into the tank body of the second fermentation tank 2 through the jet pipe A207 at the lower end thereof during the rotation of the second stirring shaft 203, and then the fermentation gas in the second fermentation tank 2 is output from the first air outlet 104 and enters the second air channel 203 in the second stirring shaft 203. The fermentation gas is output from the second gas outlet 204 and enters the third gas channel in the third stirring shaft 303 through the second gas outlet pipeline 10, and the jet pipe B307 at the lower end of the third stirring shaft 303 is ejected into the tank body of the third fermentation tank 3, and then the fermentation gas in the third fermentation tank 3 is output from the third gas outlet 304 and enters the fourth gas channel in the fourth stirring shaft 403 through the third gas outlet pipeline 11, and is output into the tank body of the fourth fermentation tank 4 through the aeration pipe 4033 at the lower end of the fourth stirring shaft 403, and finally the excess gas in the fourth fermentation tank 4 can enter the gas output pipeline and be discharged from the fourth gas outlet 404. The flow direction of the fermentation liquid is opposite to that of the gas, that is, the fermentation liquid flows along the fourth fermentation tank 4, the third fermentation tank 3, and the second fermentation tank 2, and is finally enriched in the first fermentation tank 1, and at the same time, the feed tank 6 replenishes fresh culture medium into each tank body.

[0049] The fermentation bacteria used in the present invention are gas-eating microbial strains, including Clostridiumljungdahlii, Clostridium autoethanogenum, Clostridium carboxidivorans and Acetobacterium woodii.

[0050] The present invention realizes multi-stage utilization of CO2 by connecting multiple fermentation tanks in series, and the volume of each fermentation tank is halved successively, so that the CO2 utilization efficiency can be increased to more than 90%, and the output of acetic acid / alcohol products will also be greatly increased. In addition, the present invention designs different types of stirring shaft structures according to different volume tanks to ensure the improvement of CO2 absorption utilization rate.

[0051] Several test examples are listed below to further illustrate the working principle of the present invention.

[0052] In this test example, the gas-eating microorganism strain used is Clostridium ljungdahlii, and the specific process is as follows:

[0053] 1. Inoculate 5-10% v / v of Clostridium Yongdal into an anaerobic bottle containing PETC medium, and fill the bottle with a mixture of CO2:H2 at a ratio of 6:4, and maintain the pressure at 0.12

[0054] MPa, temperature 37°C, culture 48-72 hours.

[0055] The PETC culture medium formula is:

[0056] Component A: NH4Cl 1g / L, KCl 0.1g / L, MgSO4·7H2O 0.2g / L, NaCl 0.8g / L, KH2PO4 0.1g / L;

[0057] Component B: MnSO4·H2O 0.011g / L / MnCl2·4H2O 0.013g / L, CoCl·2H2O

[0058] 0.002g / L, ZnSO4·7H2O 0.002g / L, NiCl·6H2O 0.0002g / L,

[0059] Na2WO4·2H2O 0.25mg / L, Na2SeO3 0.2mg / L, NaMoO4·2H2O 2mg / L; Component C: CaCl2·7H2O 0.023g / L;

[0060] Component D: aminotriacetic acid 0.02 g / L;

[0061] Component E: yeast powder 0.5g / L, cysteine ​​0.1g / L, cysteine ​​hydrochloride 1.5-3.0g / L, NaHCO3 0.99g / L;

[0062] Component F: Vitamins (1000x): Biotin 10 mg / L, Folic acid 20 mg / L, Vitamin B6 100 mg / L, Thiamine 250 mg / L, Riboflavin 50 mg / L, Niacin 50 mg / L, Calcium pantothenate 40 mg / L, Cyanocobalamin 50 mg / L, Para-aminobenzoic acid 50 mg / L, Lipoic acid 50 mg / L;

[0063] Prepare a stock solution and add 1 mL per liter of culture medium;

[0064] Component G: FeSO4·7H2O 4mg / L;

[0065] Adjust pH to 5.6-5.8.

[0066] 2. Single tank test.

[0067] C. jundalii was cultured to OD 600When the OD was about 0.5, it was inoculated into a 20L seed tank for expansion culture. The culture conditions were PETC medium, 37°C, gas ratio H2:CO2 was 6:4, pressure was 0.12MPa, and culture was continued until OD 600 To about 3 hours, and then inoculated into the first fermentation tank 1 through the feeding port 110 for cultivation, the fermentation tank pressure is 1.8Mpa, the inoculation ratio is 10% to 20%, the culture conditions are the same as the 20L seed tank, the initial stirring rate is 200rpm, the initial ventilation rate is maintained at 0.6L / min, when the bacteria begin to consume gas, the ventilation volume and stirring rate are gradually increased to 2L / min and 400rpm, the feeding tank 6 begins to continuously replenish fresh culture medium from the first feeding port 105, and at the same time, the excess fermentation liquid is discharged to the microfiltration device through the discharge port 109 to achieve the separation of the fermentation bacteria and the fermentation product, and the overall dilution rate is controlled at 1.0 / day. During the fermentation process, the fermentation status is monitored by temperature sensors, pH sensors, and OPR sensors. After the fermentation is completed, the fermentation liquid is passed into the filtration collection system through the discharge port 109 to collect the fermentation product.

[0068] During the fermentation period, samples were taken from the sampling port every 6 hours.

[0069] All samples were tested by spectrophotometer at 600 nm to determine the bacterial biomass, and high performance liquid chromatography (HPLC) was used to determine the product level. The detection conditions were: liquid chromatography (Agilent 1260B, Agilent Technologies, USA), equipped with a UV detector and Aminex HPX-87H column (Bio-Rad, USA), column temperature 50 ° C, mobile phase 5 mM sulfuric acid aqueous solution, flow rate 0.6 mL / min.

[0070] Tail gas analysis: The fermentation tail gas components were detected by a gas chromatograph connected to the headspace of the fermentation tank. The detection conditions were: Huifen gas chromatograph (HF 601), injection volume of 1 mL, injection port temperature of 250 ° C, chromatographic column of TDX-01 (30m × 0.32mm × 0.5μm), TCD detector temperature of 300 ° C, carrier gas (argon) flow rate of 30mL / min. The column oven temperature program was: starting at 60 ° C, then increasing at a rate of 5 ° C / min to 80 ° C (10min), and then increasing at 10 ° C / min to 170 ° C (6min), and maintaining for 1min.

[0071] The sampling interval is 15 minutes.

[0072] Experimental results:

[0073] After 7 days of fermentation, the CO2 utilization rate was 56.9% and the average daily acetic acid production was 36.4 g / L ( Fig. 9 shown).

[0074] 3. Double tank test.

[0075] The fermentation method, culture of strains, sampling and analysis methods are the same as the single tank test. The volume of each fermenter is gradually reduced. When the diameter-to-height ratio (D / H) remains unchanged, the volume of the second fermenter 2 is half of the first fermenter 1. The tank pressure is reduced successively. The tank pressure of the first fermenter 1 is 1.8Mpa, and the tank pressure of the second fermenter 2 is 1.5Mpa. The gas flow rate entering the first fermenter 1 is 2L / min, and the ventilation flow rate from the first fermenter 1 to the second fermenter 2 is set to 0.8L / min. The raw gas is first introduced into the first fermenter 1, and the fermentation tail gas of the first fermenter 1 is then introduced into the second fermenter 2, and finally discharged from the gas outlet of the second fermenter 2, and part of it is sampled and detected by gas chromatography. In addition, the fermentation liquid in the second fermentation tank 2 is passed to the first fermentation tank 1, and fresh culture medium is supplemented from the feed tank 6 to the second fermentation tank 2 and the first fermentation tank 1, maintaining the overall dilution rate at 1.0 / day, and the excess fermentation liquid is discharged to the filtration collection system through the discharge port 109, and the fermentation product is enriched in the first fermentation tank 1, and finally the fermentation bacteria and the fermentation product are separated by the microfiltration device.

[0076] After 7 days of fermentation, the CO2 utilization rate of the double tank series fermentation reached 80.2% ( Fig. 9 The average daily production of acetic acid is 44.2 g / L.

[0077] 4. Three-can test.

[0078] The fermentation method, culture of strains, sampling and analysis methods are the same. The volume of each fermentation tank is gradually reduced. When the diameter-to-height ratio (D / H) remains unchanged, the volume is half of the previous fermentation tank. The tank pressure is reduced successively. The tank pressure of the first fermentation tank 1 is 1.8Mpa, the tank pressure of the second fermentation tank 2 is 1.5Mpa, and the tank pressure of the third fermentation tank 3 is 1.2Mpa. The gas flow rate entering the first fermentation tank 1 is 3L / min, the ventilation flow rate from the first fermentation tank 1 to the second fermentation tank 2 is set to 1.2L / min, and the gas flow rate from the second fermentation tank 2 to the third fermentation tank 3 is 0.5L / min. The raw gas is passed into the first fermentation tank 1 and the second fermentation tank 2 in sequence, and finally discharged from the gas outlet of the third fermentation tank 3. The fermentation product is enriched in the first fermentation tank 1 from the third fermentation tank 3, and the feed tank 6 replenishes fresh culture medium to the third fermentation tank 3, the second fermentation tank 2 and the first fermentation tank 1, respectively, maintaining the overall dilution rate at 1.0 / day, and the excess fermentation liquid is discharged to the filtration collection system through the discharge port 109, and finally the fermentation bacteria and the fermentation product are separated by the microfiltration device.

[0079] After 7 days of fermentation, the gas utilization rate of the three-tank series fermentation reached 89.6% ( Fig. 9 The average daily production of acetic acid is 48.6 g / L.

[0080] 5. Four-can test.

[0081] The fermentation method, culture of strains, sampling and analysis methods are the same. The volume of each fermentation tank is gradually reduced. When the diameter-to-height ratio (D / H) remains unchanged, the volume is half of the previous fermentation tank, and the tank pressure is reduced successively. The tank pressure of the first fermentation tank 1 is 1.8Mpa, the tank pressure of the second fermentation tank 2 is 1.5Mpa, the tank pressure of the third fermentation tank 3 is 1.2Mpa, and the tank pressure of the fourth fermentation tank 4 is 0.9Mpa. The gas flow rate entering the first fermentation tank 1 is 4L / min, the ventilation flow rate from the first fermentation tank 1 to the second fermentation tank 2 is set to 1.6L / min, the gas flow rate from the second fermentation tank 2 to the third fermentation tank 3 is 0.6L / min, and the gas flow rate from the third fermentation tank 3 to the fourth fermentation tank 4 is 0.3L / min. The raw gas passes through the first fermentation tank 1, the second fermentation tank 2, the third fermentation tank 3, and the fourth fermentation tank 4 in sequence, and is finally discharged from the gas outlet of the fourth fermentation tank 4. The fermentation product is enriched in the first fermentation tank 1 from the fourth fermentation tank 4, and the feed tank 6 replenishes fresh culture medium to the fourth fermentation tank 4, the third fermentation tank 3, the second fermentation tank 2 and the first fermentation tank 1, respectively, maintaining the overall dilution rate at 1.0 / day, and the excess fermentation liquid is discharged to the filtration collection system through the discharge port 109, and finally the fermentation bacteria and the fermentation product are separated by the microfiltration device.

[0082] The gas utilization rate of the four-tank series fermentation reached 94.3% ( Fig. 9 The average daily production of acetic acid was 54.2 g / L.

[0083] 6. Four-tank series continuous fermentation using industrial simulated gas as raw material.

[0084] In addition to CO2 and H2, the tail gas emitted by coal chemical enterprises also contains a certain proportion of CO, CH4, N2 and other components. This embodiment uses a mixed gas of N2:H2:CO:CO2:CH4=25:35:10:20:10 to simulate the coal chemical tail gas. Continuous ventilation and feeding are used during the fermentation process. The fermentation method, activation culture of the strain, fermentation control parameters, sampling and analysis methods are the same as the four-tank series fermentation.

[0085] After 15 days of fermentation, the comprehensive utilization rate of CO2 reached 95.5%, the average daily production of acetic acid was 50.36g / L, and the average daily production of ethanol was 8.35g / L.

[0086] In addition, it can be seen from the above test examples that the present invention can flexibly start the number of fermentation tanks according to needs, and the present invention can produce one or more fermentation products according to the components and composition ratios of the gas raw materials, which can improve the flexibility of use and scope of application of the present invention.

Claims

1. A device for utilizing fermentation of gas-eating microorganisms and absorption and utilization of carbon dioxide, characterized in that: The invention comprises a first fermentation tank (1), a second fermentation tank (2), a third fermentation tank (3), a fourth fermentation tank (4) and a gas storage tank (5), wherein a first stirring shaft (103) is provided inside the first fermentation tank (1), and an aeration stirring paddle (1032) with a paddle aeration hole (10321) is provided on the first stirring shaft (103), a first airway (1034) is provided inside the first stirring shaft (103), and the gas storage tank (5) is connected to the upper end of the first airway (1034) through an air inlet pipeline (8), and a shaft wall of the first stirring shaft (103) is provided with a gas inlet pipeline (8) connected to the first airway (1034). 4) and the inner cavity of the aeration stirring blade (1032), a second stirring shaft (203) is provided inside the second fermentation tank (2), and a gas injection pipe A (207) with a plurality of gas nozzle assemblies (208) is provided at the lower end of the second stirring shaft (203), a second air channel (2033) is provided inside the second stirring shaft (203), and the upper end of the first fermentation tank (1) is connected to the upper end of the second air channel (2033) through the first gas outlet pipeline (9), and the lower end of the second air channel (2033) is connected to the inner cavity of the gas injection pipe A (207), and the third fermentation tank (3) is provided inside A third stirring shaft (303) is provided, and a gas jet pipe B (307) with a plurality of gas jet nozzles (308) is provided at the lower end of the third stirring shaft (303), a third air channel is provided inside the third stirring shaft (303), and the upper end of the second fermentation tank (2) is connected to the upper end of the third air channel through a second gas outlet pipeline (10), and the lower end of the third air channel is connected to the inner cavity of the gas jet pipe B (307), a honeycomb guide tube (309) is provided on the third stirring shaft (303), and a fourth stirring shaft (403) is provided inside the fourth fermentation tank (4), and an aeration pipe with aeration holes is provided at the lower end of the fourth stirring shaft (403). (4033), a fourth air duct is provided inside the fourth stirring shaft (403), and the upper end of the third fermentation tank (3) is connected to the upper end of the fourth air duct through the third air outlet pipeline (11), and the lower end of the fourth air duct is connected to the inner cavity of the aeration pipe (4033), the first fermentation tank (1), the second fermentation tank (2), the third fermentation tank (3) and the fourth fermentation tank (4) are connected in sequence through corresponding fermentation liquid pipelines and the volumes are halved in sequence, and the upper ends of the first fermentation tank (1), the second fermentation tank (2), the third fermentation tank (3) and the fourth fermentation tank (4) are all provided with fermentation liquid feeding pipes (701).

2. The device for utilizing fermentation of gas-eating microorganisms and absorption and utilization of carbon dioxide according to claim 1, characterized in that: The gas nozzle assembly (208) comprises a reducing nozzle (2081), a connecting frame (2085) and a spray rod (2083), wherein the spray rod (2083) is provided with a suction nozzle (2082) at one end and an output nozzle (2084) at the other end, the reducing nozzle (2081) is installed on the injection pipe A (207), and the reducing nozzle (2081) and the suction nozzle (2082) are respectively arranged on both sides of the connecting frame (2085).

3. The device for utilizing fermentation of gas-eating microorganisms and absorption and utilization of carbon dioxide according to claim 1, characterized in that: The first fermentation tank (1) is provided with a first gas outlet (104) at the upper end, and the first gas outlet (104) is connected to the upper end of the second gas channel (2033) through a first gas outlet pipeline (9); the second fermentation tank (2) is provided with a second gas outlet (204) at the upper end, and the second gas outlet (204) is connected to the upper end of the third gas channel through a second gas outlet pipeline (10); the third fermentation tank (3) is provided with a third gas outlet (304) at the upper end, and the third gas outlet (304) is connected to the upper end of the fourth gas channel through a third gas outlet pipeline (11); the fourth fermentation tank (4) is provided with a fourth gas outlet (404) at the upper end, and the fourth gas outlet (404) is connected to a gas output pipeline.

4. The device for utilizing fermentation of gas-eating microorganisms and absorption and utilization of carbon dioxide according to claim 2, characterized in that: The air inlet pipeline (8) is provided with an air inlet flow meter (801), the gas output pipeline is provided with a gas output flow meter (402), and the first air outlet pipeline (9), the second air outlet pipeline (10), and the third air outlet pipeline (11) are all provided with an air outlet flow control valve (12).

5. The device for utilizing fermentation of gas-eating microorganisms and absorption and utilization of carbon dioxide according to claim 1, characterized in that: The first fermentation tank (1) has a feeding port (101) at its upper end, a first liquid inlet (106) at one side of the first fermentation tank (1), a second liquid outlet (201) at one side of the second fermentation tank (2) and a second liquid inlet (202) at the other side, a third liquid outlet (301) at one side of the third fermentation tank (3) and a third liquid inlet (302) at the other side, and a fourth liquid outlet (401) at one side of the fourth fermentation tank (4), wherein the first liquid inlet (106) The first fermentation tank (1) is connected to the second liquid outlet (201) via a first fermentation liquid pipeline (13); the second liquid inlet (202) is connected to the third liquid outlet (301) via a second fermentation liquid pipeline (14); the third liquid inlet (302) is connected to the fourth liquid outlet (401) via a third fermentation liquid pipeline (15); and discharge ports (109) are provided at the lower ends of the first fermentation tank (1), the second fermentation tank (2), the third fermentation tank (3) and the fourth fermentation tank (4).

6. The device for utilizing fermentation of gas-eating microorganisms and absorption and utilization of carbon dioxide according to claim 5, characterized in that: The first fermentation liquid pipeline (13), the second fermentation liquid pipeline (14), and the third fermentation liquid pipeline (15) are all provided with a fermentation liquid flow meter (16) and a fermentation liquid pipeline control valve (17).

7. The device for utilizing fermentation of gas-eating microorganisms and absorption and utilization of carbon dioxide according to claim 1, characterized in that: The first fermentation tank (1) is provided with a first feed port (105) at the upper end, the second fermentation tank (2) is provided with a second feed port (205) at the upper end, the third fermentation tank (3) is provided with a third feed port (405) at the upper end, and the fourth fermentation tank (4) is provided with a fourth feed port (405) at the upper end. The first feed port (105), the second feed port (205), the third feed port (405) and the fourth feed port (405) are respectively connected to a feed main pipe (7) through corresponding fermentation liquid feed pipes (701). The feed main pipe (7) is connected to a feed tank (6). The fermentation liquid feed pipe (701) is provided with a feed flow control valve.

8. The device for utilizing fermentation of gas-eating microorganisms and absorption and utilization of carbon dioxide according to claim 1, characterized in that: The first fermentation tank (1) has a first drive assembly at its upper end for driving a first stirring shaft (103) to rotate, the second fermentation tank (2) has a second drive assembly (206) at its upper end for driving a second stirring shaft (203) to rotate, the third fermentation tank (3) has a third drive assembly (306) at its upper end for driving a third stirring shaft (303) to rotate, and the fourth fermentation tank (4) has a fourth drive assembly (406) at its upper end for driving a fourth stirring shaft (403) to rotate.

9. The device for utilizing fermentation of gas-eating microorganisms and absorption and utilization of carbon dioxide according to claim 1, characterized in that: The first drive assembly, the second drive assembly (206), the third drive assembly (306) and the fourth drive assembly (406) have the same structure and all include a stirring reduction motor (101), a transmission belt (102) and a stirring shaft adapter (1033), wherein the stirring shaft adapter (1033) is provided with a rotating shaft sleeve (10331) mounted on a corresponding pipeline, and the stirring shaft adapter (1033) is provided with a driven pulley (10322) on the outside, and a driving pulley is provided on the output shaft of the stirring reduction motor (101), and the driving pulley is connected to the driven pulley (10322) via a transmission belt (102).

10. The device for utilizing fermentation of gas-eating microorganisms and absorption and utilization of carbon dioxide according to claim 1, characterized in that: The first fermentation tank (1), the second fermentation tank (2), the third fermentation tank (3), and the fourth fermentation tank (4) are all provided with a sensor installation port (107) and a sampling port (108); the first stirring shaft (103) is also provided with a first defoaming paddle (1031), the second stirring shaft (203) is also provided with a second defoaming paddle (2031) and a stirring blade A (2032), the third stirring shaft (303) is also provided with a third defoaming paddle, and the third defoaming paddle is arranged on the upper side of the honeycomb guide tube (309), and the fourth stirring shaft (403) is also provided with a fourth defoaming paddle (4031) and a stirring blade B (4032).

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