Magnetic bead method nucleic acid extraction kit convenient for sample reservation

Abstract

The invention relates to the technical field of nucleic acid extraction, in particular to a paramagnetic particle method nucleic acid extraction kit convenient for sample reserving, which comprises a box body, an extraction hole arranged on the box body and a sample reserving piece, a clamping groove is arranged on one side of the box body, a baffle plate is slidably connected in the clamping groove, and the sample reserving piece is arranged in the clamping groove. The lower surface of the baffle is connected with the bottom of the box body through a first elastic piece. The sample reserving piece comprises a cuboid and an inserting plate, the inserting plate is arranged on one side of the cuboid and used for being inserted into the clamping groove, a plurality of sample reserving cavities are formed in the cuboid, the clamping groove, the baffle and the sample reserving piece are arranged, the inserting plate only needs to be inserted into the clamping groove, the sample reserving piece continues to be pressed downwards, the first sampling hole is communicated with the second sampling hole, and the sample reserving cavity is formed. The nucleic acid reagent in the extraction hole enters the sample reserving cavity through the second sampling hole and the first sampling hole, sample reserving is achieved, the cuboid is taken down after sample reserving is completed, sample reserving is simple, the size is small, and small space in a refrigerator is occupied.

Description

Technical Field

[0001] The invention relates to the technical field of nucleic acid extraction, and in particular to a magnetic bead method nucleic acid extraction kit which is convenient for sample retention. Background Art

[0002] The magnetic bead-based nucleic acid extraction kit is a high-tech product that combines biological science and nanomaterial science. It is a major breakthrough in my country's nucleic acid extraction and purification technology. It completely solves the long-term dependence on imports for nucleic acid extraction and purification in my country. Compared with traditional nucleic acid extraction methods, it can be combined with a fully automatic nucleic acid extractor to process multiple samples and achieve high-throughput biological operation requirements.

[0003] At present, the nucleic acid extraction kits used in the fully automatic nucleic acid extraction instruments on the market generally adopt multi-grid deep-well plates. The first column is the sample addition well, with lysis solution and magnetic beads added, the last column is the elution well, with elution solution added, and the middle columns are washing wells, with washing solution added. The magnetic bead method nucleic acid extraction steps are performed through the magnetic sleeve and magnetic rod assembly inside the fully automatic nucleic acid extraction instrument. For diseases such as influenza, avian influenza, hand, foot and mouth disease, and coronavirus, it is usually necessary to retain samples after the elution is completed for subsequent re-examination or testing of other items. Although only one nucleic acid sample is required for each row, the entire kit needs to be powdered with tape and stored in the refrigerator, which will take up a lot of space in the refrigerator. After the refrigerator is full, it is necessary to buy another refrigerator or discard the previous samples. Therefore, it is urgent to develop a magnetic bead method nucleic acid extraction kit that is convenient for sample retention and easy to store. Summary of the invention

[0004] The object of the present invention is to provide a magnetic bead method nucleic acid extraction kit that is convenient for sample retention, so as to solve the problem of inconvenience in sample retention mentioned in the background technology. The specific technical scheme is as follows:

[0005] To achieve the above-mentioned purpose and other related purposes, the present invention provides a magnetic bead method nucleic acid extraction kit that is convenient for sample retention, comprising a box body, an extraction hole arranged on the box body, and a sample retention member, wherein a card slot is arranged on one side of the box body, a baffle is slidably connected in the card slot, and the lower surface of the baffle is connected to the bottom of the box body through a first elastic member;

[0006] The sample retaining member comprises a rectangular parallelepiped and an inserting plate. The inserting plate is arranged on one side of the rectangular parallelepiped and is used to be inserted into the card slot. A plurality of sample retaining cavities are provided in the rectangular parallelepiped, and the number of the sample retaining cavities is the same as the number of the extraction holes used for elution.

[0007] Therefore, in this embodiment, there are nine sample retention cavities, and a first sampling hole is opened on the side wall of the sample retention cavity, and the first sampling hole extends outward and penetrates the plug plate;

[0008] A plurality of second sampling holes cooperating with the first sampling holes are provided on the side wall of the card slot, and the plurality of second sampling holes are respectively connected with the extraction holes on the adjacent side. The second sampling holes are used to guide the nucleic acid reagents in the extraction holes into the first sampling holes, and the baffle is used to block the second sampling holes.

[0009] Preferably, a plurality of liquid collection holes are provided on the top of the rectangular parallelepiped, and the plurality of liquid collection holes are connected to the sample retention cavity.

[0010] Preferably, the inner wall of the sample retention cavity is fixedly connected to a valve housing, a ball core is arranged in the valve housing, a guide hole is arranged on the valve housing, the ball core is in contact with the first sampling hole, one end of a spring is arranged on the side of the ball core away from the first sampling hole, the other end of the spring is arranged on the valve housing, the spring is used to push the ball core to seal the first sampling hole, the ball core is made of ferromagnetic metal material, and a positioning piece for fixing the sample retention piece is arranged in the slot.

[0011] Preferably, a second elastic member is provided on the inner wall of the slot, and the second elastic member is pressed against the baffle.

[0012] Preferably, U-shaped plates are respectively provided on both sides of the cuboid, the U-shaped plates and the plug-in plates are located at the same height, and the U-shaped plates are used for inserting the plug-in plates.

[0013] Preferably, the cuboid is made of PET material.

[0014] Preferably, the positioning member is a latch, and a socket is provided on the side wall of the slot.

[0015] Preferably, the first elastic member is a first U-shaped spring sheet, one side of the first U-shaped spring sheet is connected to the baffle, and the other side of the first U-shaped spring sheet is connected to the bottom plate of the box body.

[0016] Preferably, the second elastic member is a second U-shaped spring sheet, and both ends of the second U-shaped spring sheet are fixedly connected to the inner wall of the slot.

[0017] Beneficial effects of the present invention:

[0018] By setting the card slot, baffle and sample retention piece, you only need to insert the plug plate into the card slot and continue to press down the sample retention piece, the first sampling hole and the second sampling hole are connected, and the nucleic acid reagent in the extraction hole enters the sample retention cavity through the second sampling hole and the first sampling hole to achieve sample retention. After the sample retention is completed, the rectangular body is removed and then taped to the surface of the plug plate to seal the first sampling hole. Then the sample retention piece can be stored separately in the refrigerator. Sample retention is simple, compact, and occupies less space in the refrigerator.

[0019] By adding structures such as a valve housing and a ball core, after the fully automatic nucleic acid extractor completes the nucleic acid extraction work, only a movement program of a magnetic rod and a magnetic sleeve needs to be added to move the magnetic rod to one side of the rectangular parallelepiped. The magnetic rod adsorbs the ball core, causing the spring to be compressed, and the ball core releases the blockage of the first sampling hole. The nucleic acid reagent enters the valve housing from the second sampling hole and the first sampling hole, and flows into the sample retention cavity from the guide hole, thereby realizing automatic sample retention and making sample retention more convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 It is a schematic diagram of the overall structure of a magnetic bead method nucleic acid extraction kit for easy sample retention according to the present invention;

[0022] Figure 2 It is a schematic diagram of the structure for removing the retained sample from the card slot;

[0023] Figure 3 It is a structural schematic diagram of the retained sample;

[0024] Figure 4 It is a cross-sectional view of the retained sample;

[0025] Figure 5 It is a connection diagram of multiple sample parts;

[0026] Figure 6 yes Figure 2 A partial enlarged view of point A in the middle. DETAILED DESCRIPTION

[0027] The following is a further detailed description of a magnetic bead method nucleic acid extraction kit for convenient sample retention proposed by the present invention in conjunction with the accompanying drawings and specific embodiments. According to the following description, the advantages and features of the present invention will become clearer. It should be noted that the accompanying drawings are all in a very simplified form and are not in precise proportions, and are only used to conveniently and clearly assist in explaining the purpose of the embodiments of the present invention.

[0028] In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0029] It should be noted that the illustrations provided in the following embodiments are only used to illustrate the basic concept of the present invention in a schematic manner, and thus the illustrations only show components related to the present invention rather than being drawn according to the number, shape and size of components in actual implementation. In actual implementation, the form, quantity and proportion of each component may be changed arbitrarily, and the component layout may also be more complicated.

[0030] To this end, the present invention provides a magnetic bead method nucleic acid extraction kit that is convenient for sample retention, such as Figure 1-6 As shown, it includes a box body 100, an extraction hole 110 arranged on the box body 100, and a sample retaining member 200. A card slot 120 is arranged on one side of the box body 100, and a baffle 130 is slidably connected in the card slot 120. The lower surface of the baffle 130 is connected to the bottom of the box body 100 through a first elastic member 140.

[0031] The sample retaining member 200 includes a rectangular parallelepiped 210 and an inserting plate 220. The inserting plate 220 is provided on one side of the rectangular parallelepiped 210. The inserting plate 220 is used to be inserted into the card slot 120. A plurality of sample retaining cavities 211 are provided in the rectangular parallelepiped 210. The number of the sample retaining cavities 211 is the same as the number of the extraction holes 110 used for elution. Therefore, in this embodiment, there are nine sample retaining cavities 211. A first sampling hole 212 is provided on the side wall of the sample retaining cavity 211. The first sampling hole 212 extends outward and penetrates the inserting plate 220.

[0032] A plurality of second sampling holes cooperating with the first sampling hole 212 are provided on the side wall of the card slot 120, and the plurality of second sampling holes are respectively connected with the extraction hole 110 on the adjacent side, and the second sampling holes are used to guide the nucleic acid reagent in the extraction hole 110 into the first sampling hole 212, and the baffle 130 is used to block the second sampling holes.

[0033] When sampling is needed, the plug plate 220 only needs to be inserted into the card slot 120, the plug plate 220 contacts the baffle 130, and the sample retaining piece 200 continues to be pressed down. The baffle 130 is pressed down to release the blockage of the second sampling hole, and then the plug plate 220 moves down. After the cuboid 210 moves to the bottom, the first sampling hole 212 is connected with the second sampling hole, and the nucleic acid reagent in the extraction hole 110 enters the sample retaining cavity 211 through the second sampling hole and the first sampling hole 212 to achieve sample retention. After the sampling is completed, the cuboid 210 is removed and then taped to the surface of the plug plate 220 to seal the first sampling hole 212. Then the sample retaining piece 200 can be stored separately in the refrigerator. Sampling is simple and the volume is compact.

[0034] Furthermore, a plurality of liquid collection holes 213 are provided on the top of the cuboid 210, and the plurality of liquid collection holes 213 are connected to the sample retention cavity 211. The liquid collection holes 213 are used to serve as a connection during the sample retention operation, so as to avoid the problem that the nucleic acid reagent is difficult to pass through the first sampling hole 212 due to the air pressure in the sample retention cavity 211. After the sample retention is completed, a sealing tape is pasted on the top of the cuboid to achieve sealing.

[0035] Furthermore, the inner wall of the sample retention cavity 211 is fixedly connected with a valve housing 214, a ball core 215 is arranged in the valve housing 214, a guide hole 216 is arranged on the valve housing 214, the ball core 215 is in contact with the first sampling hole 212, one end of a spring 217 is arranged on the side of the ball core 215 away from the first sampling hole 212, the other end of the spring 217 is arranged on the valve housing 214, the spring 217 is used to push the ball core 215 to block the first sampling hole 212, the ball core 215 is made of ferromagnetic metal material, and a positioning piece 160 for fixing the sample retention piece 200 is arranged in the card slot 120; when in use, the sample retention piece 200 is first inserted into the card slot 120 and positioned by the positioning piece 160, at this time the first sampling hole 212 It is connected to the second sampling hole. At this time, the ball core 215 is blocked in the first sampling hole 212. Then the nucleic acid extraction kit is placed in the fully automatic magnetic bead nucleic acid extractor. Then the nucleic acid extractor performs normal nucleic acid extraction operations. After the extraction is completed, a movement program of a magnetic rod and a magnetic sleeve is added. The magnetic rod is moved to one side of the rectangular parallelepiped 210. The magnetic rod adsorbs the ball core 215, so that the spring 217 is compressed. The ball core 215 releases the blockage of the first sampling hole 212. The nucleic acid reagent enters the valve housing 214 from the second sampling hole and the first sampling hole 212, and flows into the sample retention cavity 211 from the guide hole 216, so that automatic sample retention can be achieved. After the sample retention part 200 is taken out, the ball core 215 blocks the first sampling hole 212 again under the push of the spring 217 to achieve closure.

[0036] Furthermore, a second elastic member 150 is provided on the inner wall of the slot 120, and the second elastic member 150 is pressed on the baffle 130 to push the baffle 130 so that the baffle 130 fits more closely to the side wall of the slot 120, thereby improving the effect of the baffle 130 in blocking the second sampling hole and avoiding liquid leakage. When the plug-in plate 220 is inserted, the second elastic member 150 will squeeze the plug-in plate 220 so that the plug-in plate 220 fits more closely to the side wall of the slot 120, thereby improving the connection tightness between the second sampling hole and the first sampling hole 212 and avoiding the nucleic acid reagent from leaking from between the first sampling hole 212 and the second sampling hole.

[0037] Furthermore, U-shaped plates 230 are respectively provided on both sides of the rectangular parallelepiped 210. The U-shaped plates 230 and the plug plates 220 are located at the same height. The U-shaped plates 230 are used for inserting the plug plates 220. By inserting another plug plate 220 between the U-shaped plates 230, multiple sample pieces 200 can be engaged, which is convenient for arrangement and storage.

[0038] Furthermore, the cuboid 210 is made of PET material, which will not affect the magnetic attraction performance of the magnetic rod to the spherical core 215.

[0039] Furthermore, the positioning member 160 is a latch, and a plug hole 170 is provided on the side wall of the slot 120. Of course, the positioning member 160 can also be a snap-fit ​​structure, a clamping structure, etc.

[0040] Furthermore, the first elastic member 140 is a first U-shaped spring sheet, one side of which is connected to the baffle 130 , and the other side of which is connected to the bottom plate of the box body 100 .

[0041] Furthermore, the second elastic member 150 is a second U-shaped spring piece, and both ends of the second U-shaped spring piece are fixedly connected to the inner wall of the slot 120 .

[0042] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A magnetic bead method nucleic acid extraction kit for easy sample retention, comprising a box body and an extraction hole arranged on the box body, characterized in that: It also includes a sample retaining member, wherein a card slot is provided on one side of the box body, a baffle is slidably connected in the card slot, and the lower surface of the baffle is connected to the bottom of the box body through a first elastic member; The sample retaining member includes a rectangular parallelepiped and an inserting plate. The inserting plate is provided on one side of the rectangular parallelepiped, and the inserting plate is used to be inserted into the card slot. A plurality of sample retaining cavities are provided in the rectangular parallelepiped, and the number of the sample retaining cavities is the same as the number of the extraction holes used for elution. Therefore, in this embodiment, there are nine sample retaining cavities, and a first sampling hole is provided on the side wall of the sample retaining cavity, and the first sampling hole extends outward and penetrates the inserting plate; A plurality of second sampling holes cooperating with the first sampling holes are provided on the side wall of the card slot, and the plurality of second sampling holes are respectively connected with the extraction holes on the adjacent side. The second sampling holes are used to guide the nucleic acid reagents in the extraction holes into the first sampling holes, and the baffle is used to block the second sampling holes.

2. A magnetic bead method nucleic acid extraction kit for easy sample retention according to claim 1, characterized in that: A plurality of liquid collection holes are provided on the top of the rectangular parallelepiped, and the plurality of liquid collection holes are connected to the sample retention cavity.

3. A magnetic bead method nucleic acid extraction kit for easy sample retention according to claim 2, characterized in that: The inner wall of the sample retention cavity is fixedly connected to a valve housing, a ball core is arranged in the valve housing, a guide hole is arranged on the valve housing, the ball core is in contact with the first sampling hole, one end of a spring is arranged on the side of the ball core away from the first sampling hole, the other end of the spring is arranged on the valve housing, the spring is used to push the ball core to block the first sampling hole, the ball core is made of ferromagnetic metal material, and a positioning piece for fixing the sample retention piece is arranged in the slot.

4. A magnetic bead method nucleic acid extraction kit for easy sample retention according to claim 3, characterized in that: A second elastic member is disposed on the inner wall of the slot, and the second elastic member is pressed against the baffle.

5. A magnetic bead method nucleic acid extraction kit for easy sample retention according to claim 4, characterized in that: U-shaped plates are respectively arranged on both sides of the cuboid, the U-shaped plates and the plugging plates are located at the same height, and the U-shaped plates are used for inserting the plugging plates.

6. A magnetic bead method nucleic acid extraction kit for easy sample retention according to claim 1, characterized in that: The cuboid is made of PET material.

7. A magnetic bead method nucleic acid extraction kit for easy sample retention according to claim 3, characterized in that: The positioning member is a latch pin, and a plug hole is arranged on the side wall of the slot.

8. A magnetic bead method nucleic acid extraction kit for easy sample retention according to claim 1, characterized in that: The first elastic member is a first U-shaped spring sheet, one side of the first U-shaped spring sheet is connected to the baffle, and the other side of the first U-shaped spring sheet is connected to the bottom plate of the box body.

9. A magnetic bead method nucleic acid extraction kit for easy sample retention according to claim 4, characterized in that: The second elastic member is a second U-shaped spring piece, and both ends of the second U-shaped spring piece are fixedly connected to the inner wall of the slot.

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