Ultraviolet LED lamp bead sterilization efficiency evaluation device and method

Abstract

The invention relates to the technical field of microbiological detection devices, in particular to an ultraviolet LED lamp bead sterilization efficiency evaluation device and method. According to the device, a lamp bead supporting rod is vertically installed on the side of a glass slide limiting platform, and a lamp bead limiting clamp is installed on the lamp bead supporting rod and is horizontal to the glass slide limiting platform; the concave disc type glass slide is placed on the glass slide limiting platform; the platform supporting legs are mounted below the glass slide limiting platform; a scale height adjusting device is arranged at the upper end of the lamp bead supporting rod and used for adjusting the height of the lamp bead supporting rod. A fixed positioning rod is arranged at the 0-scale position of the lamp bead supporting rod; an irradiance test hole is formed in the glass slide limiting platform; the lamp bead limiting clamp is provided with a clamp limiting hole, a lamp bead limiting groove and a clamp fixing opening, and the lamp bead supporting rod is sleeved with the lamp bead limiting clamp through the clamp limiting hole. And the lamp bead limiting groove is used for placing a test lamp bead. The method has the advantages that errors of operation or random factors are reduced; the reliability of evaluating the efficiency of the ultraviolet lamp beads for inactivating the microorganisms is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial detection devices, and in particular to a device and method for evaluating the sterilization efficiency of ultraviolet LED lamp beads. Background Art

[0002] Because ultraviolet light has the characteristics of short wavelength and high photon energy, it can effectively destroy the DNA / RNA genetic material or protective structure in viruses and bacteria and achieve the purpose of inactivating microorganisms. It is regarded as a broad-spectrum, high-efficiency light source for inactivating viruses and bacteria. Mercury lamp ultraviolet light sources will gradually withdraw from the stage of history, while semiconductor-based ultraviolet LED devices have the advantages of small size, concentrated luminous wavelength, no ozone generation, and environmental friendliness. They are the main light source to replace mercury lamps in the field of microbial inactivation and will play a core role in medical and other fields.

[0003] As semiconductor ultraviolet LED lamp beads are gradually applied in the field of microbial inactivation, accurate detection of the inactivation efficiency of LED lamp beads from different manufacturers and batches has become a focus. The main factors affecting the inactivation efficiency of deep ultraviolet LED lamp beads are as follows: 1. The wavelength of the lamp bead: Due to the differences in the protective structure of different microorganisms, different types of genetic material, different sizes and other factors, they have different inactivation sensitivities to different wavelengths; 2. The ultraviolet light power density (i.e. irradiance) received by the plane where the microorganism is located: Under the same wavelength irradiation, the higher the irradiance received by the microorganism, the better the inactivation effect; 3. Other environmental factors: such as the distance between the microorganism and the lamp bead, the environmental humidity, etc., mainly affect the inactivation efficiency by affecting the irradiance received by the microorganism.

[0004] However, the existing UV lamp bead inactivation method has the problem that the inactivation detection process is not uniform and accurate. The process is affected by a variety of random factors such as the operator's technique, the dosage of the microbial droplets used, and the shape of the droplets, which reduces the reliability of the evaluation of the inactivation effect of LED products. It is not conducive to the accurate testing and comparison of the inactivation efficiency of different lamp beads. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a device and method for evaluating the sterilization efficiency of ultraviolet LED lamp beads.

[0006] The first object of the present invention is to provide an ultraviolet LED lamp bead sterilization efficiency evaluation device, comprising: a slide limiting platform, a lamp bead support rod, a concave disc type slide, a lamp bead limiting fixture and a platform support leg; The lamp bead support rod is vertically installed on the side of the slide limiting platform, and the lamp bead limiting fixture is installed on the lamp bead support rod and is horizontal to the slide limiting platform; the concave disc type slide is placed on the slide limiting platform; the platform support leg is installed under the slide limiting platform to support the slide limiting platform; The upper end of the lamp bead support rod is provided with a scale height adjustment device for adjusting the height of the lamp bead support rod; a fixed positioning rod is provided at the 0 scale position of the lamp bead support rod; The slide limiting platform is provided with an irradiance test hole, which is used to align the light emission center of the lamp bead with the center of the concave disc slide; the size of the irradiance test hole matches the ultraviolet irradiator used; The lamp bead limiting clamp is provided with a clamp limiting hole, a lamp bead limiting groove and a clamp fixing port, and is connected to the lamp bead support rod through the clamp limiting hole; after the lamp bead limiting clamp is adjusted to a predetermined position along the lamp bead support rod, the lamp bead limiting clamp is fixed to the lamp bead support rod by locking the clamp fixing port; the lamp bead limiting groove is used to place the test lamp bead to ensure that the position of the lamp bead is unified each time it is tested.

[0007] Preferably, a concave disc-shaped slide glass is provided with a concave disc and a liquid collecting groove, wherein the concave disc is used to contain microbial liquid, and the liquid collecting groove is used to collect excess liquid.

[0008] Preferably, the area of ​​the concave disk is the same as the effective detection area of ​​the ultraviolet irradiator used, and the diameter of the concave disk does not exceed 1 cm and the depth does not exceed 1 mm.

[0009] Preferably, a slide positioning rod is provided on one side of the concave disc-shaped slide, and the slide positioning rod extends to the fixed positioning rod of the lamp bead support rod; by comparing whether the slide positioning rod and the upper surface of the fixed positioning rod are horizontal, it is determined whether the upper surface of the microbial liquid is aligned with the 0 scale of the lamp bead support rod.

[0010] Preferably, the fixed positioning rod is connected to the detachable positioning rod; the detachable positioning rod is used to compare whether the 0 scale line of the lamp bead support rod and the upper surface of the ultraviolet irradiator are on the same plane.

[0011] Preferably, the sterilization is the inactivation of viruses or bacteria.

[0012] The second object of the present invention is to provide a method for evaluating the sterilization efficiency of ultraviolet LED lamp beads, using the above-mentioned device for evaluation, comprising the following steps: S1. Fix the photosensitive surface of the UV irradiator at the irradiance test port of the slide limit platform, and use the fixed positioning rod of the lamp bead support rod to align the photosensitive surface of the UV irradiator with the 0 scale position of the lamp bead support rod; S2. Adjust the lamp bead limit fixture to the specified scale, turn on the lamp bead using the test required current, measure the lamp bead irradiance under experimental conditions and record it; S3. Place the concave disc-shaped slide on the slide limiting platform, and use the slide positioning rod to align the upper surface of the concave disc-shaped slide with the 0 scale position of the lamp support rod; S4. Align the center of the concave plate with the center of the lamp bead; drop the microbial liquid into the concave plate and use the flat plate to hang off the excess liquid; S5. Turn on the lamp beads to perform inactivation experiments; S6. Turn off the lamp beads after the experiment, absorb a certain amount of inactivated microbial liquid and conduct culture or biological tissue infection experiments to confirm the microbial inactivation rate of the lamp beads; safely handle the materials that have come into contact with the microbial liquid.

[0013] Preferably, in step S4, the volume of the microbial liquid dripped into the concave dish is at least 5% larger than the volume of the concave dish to ensure that the microbial liquid fills the concave dish; excess liquid is scraped into the liquid collection tank of the concave dish-type slide.

[0014] Preferably, in step S3, the detachable positioning rod on the lamp bead support rod is first removed, the concave disc type slide is placed on the slide limiting platform, and the slide positioning rod is used to make the upper surface of the concave disc type slide flush with the upper surface of the fixed positioning rod.

[0015] Preferably, the flat plate is a disposable flat plate made of hydrophobic material.

[0016] Compared with the prior art, the present invention can achieve the following beneficial effects: Through the strict limit operation of the device, errors caused by random factors such as the test operator's technique, solution dosage, microbial droplet dosage, droplet shape, etc. can be prevented, thereby increasing the reliability of the evaluation of the efficiency of ultraviolet lamp beads in inactivating microorganisms. Different batches of lamp beads can be evaluated with very small errors. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of a UV LED lamp bead sterilization efficiency evaluation device provided according to an embodiment of the present invention.

[0018] Figure 2 Schematic diagram of the structure of a glass slide limiting platform provided according to an embodiment of the present invention.

[0019] Figure 3 Schematic diagram of the structure of a concave disc type slide glass provided according to an embodiment of the present invention.

[0020] Figure 4 Schematic diagram of the structure of a lamp bead limiting clamp provided according to an embodiment of the present invention.

[0021] Reference numerals: 1. Slide limiting platform; 101. Irradiance test hole; 2. Lamp support rod; 201. Scale height adjustment device; 202. Fixed positioning rod; 203. Removable positioning rod; 3. Concave disc slide; 301. slide positioning rod; 302. concave plate; 303. liquid collection tank; 4. Lamp bead limiting fixture; 401. Fixture fixing port; 402. Fixture limiting hole; 403. Lamp bead limiting groove; 5. Platform support legs. DETAILED DESCRIPTION

[0022] Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings. In the following description, the same modules are represented by the same reference numerals. In the case of the same reference numerals, their names and functions are also the same. Therefore, the detailed description thereof will not be repeated.

[0023] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and do not constitute a limitation of the present invention.

[0024] See also Figure 1-Figure 4 The present invention provides an ultraviolet LED lamp bead sterilization efficiency evaluation device, comprising: a slide limiting platform 1, a lamp bead support rod 2, a concave disc type slide 3, a lamp bead limiting fixture 4 and a platform support leg 5; The lamp bead support rod 2 is vertically mounted on the side of the slide limiting platform 1, and the lamp bead limiting fixture 4 is mounted on the lamp bead support rod 2 and is horizontal to the slide limiting platform 1; the lamp bead limiting fixture 4 can move up and down along the direction of the lamp bead support rod 2 and can be completely fixed at a selected position; The concave disc type glass slide 3 is placed on the glass slide limiting platform 1; The platform support legs 5 are installed below the slide limiting platform 1 and are used to support the slide limiting platform 1; The upper end of the lamp bead support rod 2 is provided with a scale height adjustment device 201 for adjusting the height of the lamp bead support rod 2; a fixed positioning rod 202 is provided at the 0 scale position of the lamp bead support rod 2, and the fixed positioning rod 202 is connected to the detachable positioning rod 203; A concave plate 302 and a liquid collecting groove 303 are provided on the concave plate type slide glass 3. The concave plate 302 is used to hold microbial liquid, and the liquid collecting groove 303 is used to collect excess liquid. A slide glass positioning rod 301 is provided on one side of the concave plate type slide glass 3. The slide glass positioning rod 301 extends to the fixed positioning rod 202 of the lamp bead support rod 2. By comparing whether the upper surface of the slide glass positioning rod 301 is horizontal with the upper surface of the fixed positioning rod 202, it can be determined whether the upper surface of the microbial liquid surface is aligned with the 0 scale of the lamp bead support rod 2. Preferably, the area of ​​the concave disk 302 is the same as the effective detection area of ​​the ultraviolet irradiator used, but the maximum diameter does not exceed 1 cm; the depth of the concave disk 302 is not greater than 1 mm; An irradiance test hole 101 is provided on the slide limiting platform 1, and the irradiance test hole 101 is used to align the light emission center of the lamp bead with the center of the concave disk 302 on the concave disk type slide 3; the size of the irradiance test hole 101 is consistent with the ultraviolet radiation meter used; The lamp bead limiting fixture 4 is provided with a fixture limiting hole 402, a lamp bead limiting groove 403 is provided on one side, and a fixture fixing port 401 is provided on the other side; the lamp bead limiting fixture 4 is sleeved on the lamp bead support rod 2 through the fixture limiting hole 402, which can prevent the lamp bead limiting fixture 4 from shaking left and right; after the lamp bead limiting fixture 4 is adjusted to a predetermined position along the lamp bead support rod 2, the lamp bead limiting fixture 4 is fixed to the lamp bead support rod 2 by locking the fixture fixing port 401; a test lamp bead is placed in the lamp bead limiting groove 403 to ensure that the position of the lamp bead remains uniform each time the test is performed; When conducting an experiment to evaluate the efficiency of microorganism inactivation of ultraviolet lamp beads, firstly, the photosensitive surface of the ultraviolet irradiator is passed through the irradiance test hole 101, and the detachable positioning rod 203 is connected to the fixed positioning rod 202, and the scale height adjustment device 201 is used to make the 0 scale line of the lamp bead support rod 2 with scale and the upper surface of the ultraviolet irradiator on the same plane; After removing the UV irradiator, remove the detachable positioning rod 203, place the concave disc type slide 3 on the slide limiting platform 1, adjust the height of the lamp bead support rod 2, so that the upper surface of the fixed positioning rod 202 is flush with the upper surface of the slide positioning rod 301, so that the upper surface of the slide concave disc 302 is aligned with the 0 scale position of the lamp bead support rod 2. At this time, the distance between the lamp bead and the upper surface of the microbial droplet is equal to the distance between the lamp bead and the photosensitive surface of the irradiator.

[0025] The purpose of the lamp bead support rod 2 is to keep the distance between the photosensitive surface of the ultraviolet irradiator and the upper surface of the microbial droplet and the lamp bead consistent, so as to make the irradiance of the microbial liquid consistent with the test value of the ultraviolet irradiator.

[0026] The arrangement of the lamp bead limiting groove 403 and the glass slide limiting platform 1 is to align the luminous center of the lamp bead with the irradiance test port at the center of the glass slide concave plate 302; to align the detection center of the ultraviolet irradiator and the center of the concave plate 302 with the luminous center of the lamp bead, so as to eliminate the test error caused by the deviation of the position of the lamp bead and the microbial droplet in different batches of tests. After the above steps are completed, a slight excess of microbial liquid is dripped into the glass slide concave plate 302 through a pipette, and the excess liquid is swept into the liquid collection tank 303 using a flat liquid plate, and then the ultraviolet lamp bead can be turned on for the microbial inactivation experiment. Based on the above steps, the relative positions between the photosensitive surface of the irradiator, the microbial liquid surface and the luminous surface of the lamp bead can be kept consistent each time the test is performed.

[0027] The present invention provides a method for evaluating the sterilization efficiency of ultraviolet LED lamp beads, which is evaluated by using the above device. The core idea of ​​the lamp bead inactivation experimental method using the device is to strictly limit the shape of microbial droplets, control the distance between the lamp bead and the microbial liquid surface, and strictly limit the microbial droplets and the lamp bead to be on the same vertical line, thereby eliminating the influence of various random factors on the evaluation of microbial inactivation efficiency.

[0028] The method specifically comprises the following steps: S1. Fix the photosensitive surface of the UV irradiator to the irradiance test port 101 of the slide limiting platform 1, and use the fixed positioning rod 202 of the lamp support rod 2 to align the photosensitive surface of the UV irradiator with the 0 scale position of the lamp support rod 2; S2. Adjust the lamp bead limit fixture 4 to the specified scale, turn on the lamp bead using the test required current, measure the lamp bead irradiance under experimental conditions and record it; S3. Place the concave disc-shaped slide 3 on the slide limiting platform 1, and use the slide positioning rod 301 to align the upper surface of the concave disc-shaped slide 3 with the 0 scale position of the lamp support rod 2; S4. Align the center of the concave plate 302 with the center of the lamp bead; drip the microbial liquid into the concave plate 302, and use the flat plate to sweep the excess liquid into the liquid collection tank 303, and then safely dispose of the flat plate; S5. Turn on the lamp beads to perform inactivation experiments; S6. After the experiment is over, turn off the lamp beads, use a pipette to absorb a certain amount of inactivated microbial liquid, and then carry out culture or biological tissue infection experiments according to normal procedures, and compare with the control group to confirm the microbial inactivation rate of the lamp beads; safely dispose of the materials that have been in contact with the microbial droplets, such as the concave disc-shaped slide 3.

[0029] In a specific embodiment, in step S3, the detachable positioning rod 203 on the lamp bead support rod 2 is first removed, the concave disc type glass slide 3 is placed on the glass slide limiting platform 1, and the glass slide positioning rod 301 is used to make the upper surface of the concave disc type glass slide 3 flush with the upper surface of the fixed positioning rod 202.

[0030] In a specific embodiment, the flat plate is a disposable flat plate made of hydrophobic material, the purpose of which is to unify the shape and thickness of microbial droplets and prevent the random shape of the droplets from affecting the test results.

[0031] In a specific embodiment, the volume of the microbial liquid dripped into the concave dish 302 should be at least 5% larger than the volume of the concave dish 302 to ensure that the microbial liquid can fill the concave dish 302; the excess liquid is scraped into the liquid collection tank 303; The liquid collection tank 303 is used in conjunction with a flat liquid plate made of a hydrophobic material. When culture fluid is dripped into the concave plate 302 of the slide glass, the surface tension causes the liquid to assume a convex shape, and the shape is relatively random due to factors such as vibration, which will affect the irradiance of the received ultraviolet light. Therefore, by dripping a slightly excessive amount of culture fluid and using the flat liquid plate, the convex portion can be smoothed out, making it completely consistent with the size and shape of the concave plate 302, thereby eliminating experimental errors.

[0032] It should be understood that the various forms of processes shown above can be used to reorder, add or delete steps. For example, the steps described in the disclosure of the present invention can be performed in parallel, sequentially or in different orders, as long as the desired results of the technical solution disclosed in the present invention can be achieved, and this document does not limit this.

[0033] The above specific implementations do not constitute a limitation on the protection scope of the present invention. It should be understood by those skilled in the art that various modifications, combinations, sub-combinations and substitutions can be made according to design requirements and other factors. Any modification, equivalent substitution and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A UV LED lamp bead sterilization efficiency evaluation device, characterized in that: include: Glass slide limiting platform, lamp bead support rod, concave disc type glass slide, lamp bead limiting fixture and platform support legs; The lamp bead support rod is vertically installed on the side of the slide limiting platform, and the lamp bead limiting fixture is installed on the lamp bead support rod and is horizontal to the slide limiting platform; the concave disc type slide is placed on the slide limiting platform; the platform support leg is installed under the slide limiting platform to support the slide limiting platform; The upper end of the lamp bead support rod is provided with a scale height adjustment device for adjusting the height of the lamp bead support rod; a fixed positioning rod is provided at the 0 scale position of the lamp bead support rod; The slide limiting platform is provided with an irradiance test hole, which is used to align the light emission center of the lamp bead with the center of the concave disc slide; the size of the irradiance test hole matches the ultraviolet irradiator used; The lamp bead limiting clamp is provided with a clamp limiting hole, a lamp bead limiting groove and a clamp fixing port, and is connected to the lamp bead support rod through the clamp limiting hole; after the lamp bead limiting clamp is adjusted to a predetermined position along the lamp bead support rod, the lamp bead limiting clamp is fixed to the lamp bead support rod by locking the clamp fixing port; the lamp bead limiting groove is used to place the test lamp bead to ensure that the position of the lamp bead is unified each time it is tested.

2. The UV LED lamp bead sterilization efficiency evaluation device according to claim 1, characterized in that: The concave disc type slide glass is provided with a concave disc and a liquid collecting groove, wherein the concave disc is used to contain microbial liquid, and the liquid collecting groove is used to collect excess liquid.

3. The UV LED lamp bead sterilization efficiency evaluation device according to claim 2, characterized in that: The area of ​​the concave disk is the same as the effective detection area of ​​the ultraviolet irradiator used, and the diameter of the concave disk does not exceed 1 cm and the depth does not exceed 1 mm.

4. A UV LED lamp bead sterilization efficiency evaluation device according to any one of claims 1 to 3, characterized in that: A slide positioning rod is provided on one side of the concave disc slide, and the slide positioning rod extends to the fixed positioning rod of the lamp bead support rod; by comparing whether the slide positioning rod is level with the upper surface of the fixed positioning rod, it is determined whether the upper surface of the microbial liquid is aligned with the 0 scale of the lamp bead support rod.

5. The UV LED lamp bead sterilization efficiency evaluation device according to claim 1, characterized in that: The fixed positioning rod is connected to the detachable positioning rod; the detachable positioning rod is used to compare whether the 0 scale line of the lamp bead supporting rod and the upper surface of the ultraviolet irradiator are on the same plane.

6. The UV LED lamp bead sterilization efficiency evaluation device according to claim 1, characterized in that: The sterilization is to inactivate viruses or bacteria.

7. A method for evaluating the sterilization efficiency of ultraviolet LED lamp beads, using the device described in any one of claims 1 to 5 for evaluation, characterized in that: The steps include: S1. Fix the photosensitive surface of the UV irradiator at the irradiance test port of the slide limit platform, and use the fixed positioning rod of the lamp bead support rod to align the photosensitive surface of the UV irradiator with the 0 scale position of the lamp bead support rod; S2. Adjust the lamp bead limit fixture to the specified scale, turn on the lamp bead using the test required current, measure the lamp bead irradiance under experimental conditions and record it; S3. Place the concave disc-shaped slide on the slide limiting platform, and use the slide positioning rod to align the upper surface of the concave disc-shaped slide with the 0 scale position of the lamp support rod; S4. Align the center of the concave plate with the center of the lamp bead; Drop the microbial liquid into the concave dish and use a flat plate to hang off the excess liquid; S5. Turn on the lamp beads to perform inactivation experiments; S6. Turn off the lamp beads after the experiment, absorb a certain amount of inactivated microbial liquid and conduct culture or biological tissue infection experiments to confirm the microbial inactivation rate of the lamp beads; safely handle the materials that have come into contact with the microbial liquid.

8. A method for evaluating the sterilization efficiency of ultraviolet LED lamp beads according to claim 7, characterized in that: In step S4, the volume of the microbial liquid dripped into the concave dish is at least 5% larger than the volume of the concave dish, ensuring that the microbial liquid fills the concave dish; the excess liquid is scraped into the liquid collection tank of the concave dish type slide.

9. A method for evaluating the sterilization efficiency of ultraviolet LED lamp beads according to claim 7, characterized in that: In step S3, the detachable positioning rod on the lamp bead support rod is first removed, the concave disc type slide is placed on the slide limiting platform, and the slide positioning rod is used to make the upper surface of the concave disc type slide flush with the upper surface of the fixed positioning rod.

10. A method for evaluating the sterilization efficiency of ultraviolet LED lamp beads according to claim 7, characterized in that: The flat liquid plate is a disposable flat liquid plate made of a hydrophobic material.

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