High performance liquid chromatograph

Abstract

The invention relates to the technical field of chromatographic instruments, in particular to a high performance liquid chromatograph which comprises a chromatographic instrument body, a reagent bottle storage box is fixedly mounted at the upper end of the chromatographic instrument body, a sample injection pipe head is mounted in the chromatographic instrument body, and the upper end of the sample injection pipe head extends into the reagent bottle storage box. A reagent bottle fixing assembly is installed at the bottom of the reagent bottle storage box, a purification assembly is arranged on the side portion of the reagent bottle fixing assembly, and the purification assembly is fixedly installed on the inner side wall of the reagent bottle storage box. Meanwhile, the upper end and the lower end of the reagent bottle are flexibly clamped and fixed, the stability of the reagent bottle in the detection process is guaranteed, meanwhile, a purification structure is matched, air in the storage box can be purified in time, the situation that different reagents are crossed and mixed during detection is avoided, and the accuracy of final atlas data is improved.

Description

Technical Field

[0001] The invention relates to the technical field of chromatographs, in particular to a high performance liquid chromatograph. Background Art

[0002] High performance liquid chromatography uses the difference in adsorption force, distribution coefficient or other affinity between the components in the sample in two incompatible phases (stationary phase and mobile phase) as the basis for separation. Its working principle is: the mobile phase in the reservoir is pumped into the system by a high-pressure pump, the sample solution enters the mobile phase through the injector, and is loaded into the chromatographic column by the mobile phase. The chromatographic column is equipped with a stationary phase. Due to the different distribution coefficients of the components of the sample solution in the two phases, when they move relative to each other in the two phases, after repeated adsorption-analysis distribution processes, the components have a large difference in movement speed, and are separated into individual components and flow out of the column in turn. After these outflowing components pass through the detector, their sample concentrations are converted into electrical signals and transmitted to the recorder, and then printed out in the form of a spectrum.

[0003] At present, in actual use, the existing liquid chromatographs usually store the reagent bottles in the PCR plate on the top of the chromatograph. The size of the holes on the PCR plate for placing the reagent bottles is fixed, and it is impossible to place reagent bottles of different sizes. At the same time, during the detection process, the traction of the injection system will cause the reagent bottles to overturn, thereby affecting the smoothness of the detection process. In addition, in order to avoid negative pressure inside the reagent bottle during detection, a sealing film is usually used at the end for incomplete sealing. Even so, if the test sample is a volatile reagent, the inside of the storage box will be contaminated, and cross-mixing may occur when two different reagents are detected, thereby reducing the accuracy of the final chromatographic data. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a high performance liquid chromatograph, which ensures the stability of the reagent bottles during the detection process by providing a structure that can store reagent bottles of different sizes and flexibly clamping and fixing the upper and lower ends of the reagent bottles. At the same time, in conjunction with the purification structure, the air inside the storage box can be purified in time, avoiding cross-mixing when detecting different reagents, thereby improving the accuracy of the final spectrum data.

[0005] The technical solution adopted by the present invention to solve the above technical problems is: a high performance liquid chromatograph, including a chromatograph body, a reagent bottle storage box is fixedly installed on the upper end of the chromatograph body, a sample injection tube head is installed inside the chromatograph body, and the upper end of the sample injection tube head extends into the reagent bottle storage box, a reagent bottle fixing assembly is installed at the bottom of the reagent bottle storage box, a purification assembly is arranged on the side of the reagent bottle fixing assembly, and the purification assembly is fixedly installed on the inner side wall of the reagent bottle storage box; the reagent bottle fixing assembly includes a base, a base is fixedly arranged in the middle of the bottom of the reagent bottle storage box, a mounting groove is provided on the upper end surface of the base, an inverted T-shaped slide is rotatably arranged in the mounting groove, a cylindrical chamber is provided in the middle of the upper end of the T-shaped slide, an annular skirt is fixedly arranged at the position near the lower end of the edge of the T-shaped slide, and an L-shaped support rod is evenly installed along its circumference at the position above the annular skirt on the side wall of the T-shaped slide, and the end of the L-shaped support rod away from the T-shaped slide is fixedly connected with a bottom fixing unit, an electric push rod is fixedly installed in the cylindrical chamber, and an upper end clamping unit is fixedly connected to the upper end of the electric push rod.

[0006] Preferably, a fixing plate is provided in the middle of the upper end of the reagent bottle storage box, and cover plates are symmetrically hinged on both sides of the fixing plate, and a ball head handle rod is fixedly installed at a position in the middle of the upper end surface of the cover plate away from the fixing plate.

[0007] Preferably, the upper end surface of the base is provided with limiting holes evenly distributed along its circumference near the edge, a cylindrical through hole is provided above the limiting hole, the annular skirt and the L-shaped support rod are penetrated by the cylindrical through hole, and the cylindrical through hole and the limiting hole correspond one to one.

[0008] Preferably, the outer edge of the upper end surface of the annular skirt is provided with scale lines along its circumference.

[0009] Preferably, the bottom fixing unit includes a support plate, and the end of the L-shaped support rod away from the T-shaped slide is fixedly connected to the support plate, and the upper end surface of the support plate is evenly provided with rectangular sliding grooves along its circumference, and a clamping rod is slidably installed in the rectangular sliding groove through a limit spring, and an arc-shaped flexible clamping block is fixedly connected to the upper end of the clamping rod, and the concave arc surfaces of the arc-shaped flexible clamping blocks are all facing the middle of the support plate.

[0010] Preferably, a buffer protrusion is symmetrically arranged in the middle of the upper end surface of the support plate and between each rectangular slide groove, and the interior of the buffer protrusion is a hollow structure.

[0011] Preferably, the upper end surface of the arc-shaped flexible clamping block is in a downwardly inclined arc surface structure near the concave arc surface, so as to facilitate the bottom of the reagent bottle to separate the arc-shaped flexible clamping block.

[0012] Preferably, the upper end clamping unit includes a mounting column, the upper end of the electric push rod is fixedly connected to the mounting column, the side wall of the mounting column is evenly provided with strip slide grooves along its circumference, a sliding rod is slidably arranged in the strip slide groove, a preload spring is fixedly installed between the upper end of the sliding rod and the top of the strip slide groove, the end of the sliding rod away from the mounting column is fixedly connected with a hollow pressure cap, and the side wall of the mounting column near the lower end is evenly installed with L-shaped limit rods along its circumference, and the lower end of the L-shaped limit rod is aligned with the cylindrical through hole and the limit hole.

[0013] Preferably, the purification component includes a purification cylinder, which is fixedly installed on the inner wall of the reagent bottle storage box, a piston rod is slidably installed inside the purification cylinder, a purification plate is fixedly installed below the piston rod, an air inlet pipe is installed in the middle of the lower end of the purification cylinder, a No. 1 one-way valve is provided on the air inlet pipe, an air inlet hood is installed at the end of the air inlet pipe, an air outlet pipe is connected to the side wall of the purification cylinder near the lower end, the air outlet pipe extends to the outside of the reagent bottle storage box and a No. 2 one-way valve is installed on the air outlet pipe.

[0014] Preferably, the upper end of the piston rod is fixedly connected with a linkage plate, the linkage plate is connected to the telescopic end of the reciprocating cylinder at a position away from the end of the piston rod, and the lower end of the reciprocating cylinder is fixedly installed at the bottom of the reagent bottle storage box.

[0015] Beneficial effects of the present invention:

[0016] 1. The reagent bottle fixing assembly can support and pre-tighten the lower ends of reagent bottles of different sizes through the bottom fixing unit, and cooperate with the upper end clamping unit that can clamp the upper ends of reagent bottles of different sizes, thereby ensuring the stability of the reagent bottles during the detection process. The fixing methods are all flexible, which can avoid damage to the reagent bottles.

[0017] 2. The electric push rod can drive the upper clamping unit downward to clamp and fix the reagent bottle while forming a negative pressure inside the purification component, which can purify the air inside the storage box in time, avoiding cross-mixing when detecting different reagents and improving the accuracy of the final spectrum data. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0019] Figure 1 It is a three-dimensional structural schematic diagram of the present invention; Figure 2 The present invention Figure 1 Schematic diagram of the three-dimensional structure after removing the chromatograph body; Figure 3 The present invention Figure 2 A schematic diagram of the three-dimensional structure after removing the cover plate and the ball handle bar; Figure 4 The present invention Figure 3A schematic diagram of the three-dimensional structure after removing the fixing plate and part of the side wall of the reagent bottle storage box; Figure 5 It is a schematic diagram of the three-dimensional connection structure of the reagent bottle fixing assembly in the present invention; Figure 6 It is a schematic diagram of the three-dimensional structure of the bottom fixing unit in the present invention; Figure 7 It is a schematic diagram of the three-dimensional structure of the upper end pressing unit in the present invention; Figure 8 It is a schematic diagram of the three-dimensional structure of the purification component in the present invention.

[0020] In the figure: 1. Chromatograph body; 2. Reagent bottle storage box; 21. Fixing plate; 22. Cover plate; 23. Ball handle bar; 3. Sample injection tube head; 4. Reagent bottle fixing assembly; 41. Base; 42. T-shaped slide seat; 43. Annular skirt; 44. L-shaped support rod; 45, bottom fixing unit; 451, support plate; 4511, buffer protrusion; 452, rectangular slide groove; 453, limit spring; 454, clamping rod; 455, arc-shaped flexible clamping block; 46. ​​Electric push rod; 47, upper end clamping unit; 471, mounting column; 472, strip slide; 473, preload spring; 474, slide rod; 475, hollow pressure cap; 476, L-shaped limit rod; 5. Purification assembly; 51. Purification cylinder; 52. Piston rod; 521. Linkage plate; 522. Reciprocating cylinder; 53. Purification plate; 54. Air inlet pipe; 55. No. 1 one-way valve; 56. Air inlet hood; 57. Air outlet pipe; 58. No. 2 one-way valve. DETAILED DESCRIPTION

[0021] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0022] Embodiment 1:

[0023] See also Figures 1 to 8 A high performance liquid chromatograph comprises a chromatograph body 1, a reagent bottle storage box 2 is fixedly installed on the upper end of the chromatograph body 1, an injection tube head 3 is installed inside the chromatograph body 1, and the upper end of the injection tube head 3 extends into the reagent bottle storage box 2, and a reagent bottle fixing component 4 is installed at the bottom of the reagent bottle storage box 2.

[0024] A fixing plate 21 is arranged in the middle of the upper end of the reagent bottle storage box 2, and cover plates 22 are symmetrically hinged on both sides of the fixing plate 21. A ball handle rod 23 is fixedly installed in the middle of the upper end surface of the cover plate 22 away from the fixing plate 21.

[0025] The reagent bottle fixing assembly 4 includes a base 41, which is fixedly arranged in the middle of the bottom of the reagent bottle storage box 2, and an installation groove is provided on the upper end surface of the base 41, in which an inverted T-shaped slide 42 is rotatably arranged, and a cylindrical chamber is provided in the middle of the upper end of the T-shaped slide 42, and an annular skirt 43 is fixedly arranged at the edge of the T-shaped slide 42 near the lower end, and an L-shaped support rod 44 is evenly installed along the circumference of the side wall of the T-shaped slide 42 above the annular skirt 43, and the end of the L-shaped support rod 44 away from the T-shaped slide 42 is fixedly connected to a bottom fixing unit 45, and an electric push rod 46 is fixedly installed in the cylindrical chamber, and an upper end clamping unit 47 is fixedly connected to the upper end of the electric push rod 46.

[0026] The upper end surface of the base 41 is evenly provided with limiting holes near the edge along its circumference, and a cylindrical through hole is arranged above the limiting hole. The annular skirt 43 and the L-shaped support rod 44 are penetrated by the cylindrical through hole, and the cylindrical through hole and the limiting hole correspond one to one.

[0027] The outer edge of the upper end surface of the annular skirt 43 is provided with scale lines along its circumference.

[0028] The bottom fixing unit 45 includes a support plate 451, and the end of the L-shaped support rod 44 away from the T-shaped slide seat 42 is fixedly connected to the support plate 451, and the upper end surface of the support plate 451 is evenly provided with rectangular sliding grooves 452 along its circumference, and a clamping rod 454 is slidably installed in the rectangular sliding groove 452 through a limit spring 453, and the upper end of the clamping rod 454 is fixedly connected to an arc-shaped flexible clamping block 455, and the concave arc surfaces of the arc-shaped flexible clamping blocks 455 are all facing the middle of the support plate 451.

[0029] A buffer protrusion 4511 is symmetrically arranged in the middle of the upper end surface of the support plate 451 and between each rectangular slide groove 452 , and the buffer protrusion 4511 is hollow inside.

[0030] The upper end surface of the arc-shaped flexible clamping block 455 is in a downwardly inclined arc surface structure near the concave arc surface, so that the arc-shaped flexible clamping block 455 can be easily placed on the bottom of the reagent bottle.

[0031] The upper end clamping unit 47 includes a mounting column 471, the upper end of the electric push rod 46 is fixedly connected to the mounting column 471, the side wall of the mounting column 471 is evenly provided with a strip slide groove 472 along its circumference, a slide rod 474 is slidably arranged in the strip slide groove 472, a preload spring 473 is fixedly installed between the upper end of the slide rod 474 and the top of the strip slide groove 472, the end of the slide rod 474 away from the mounting column 471 is fixedly connected with a hollow pressure cap 475, and the side wall of the mounting column 471 is evenly provided with an L-shaped limit rod 476 along its circumference near the lower end, and the lower end of the L-shaped limit rod 476 is aligned with the cylindrical through hole and the limit hole.

[0032] In this embodiment, the upper end of the injection tube head 3 near the end is a flexible tube. When the injection tube head 3 is in use, the flexible tube can cooperate with the injection tube head 3 so that it can be plugged into the reagent bottle.

[0033] During specific operation, before the high performance liquid chromatograph is operated, the right side ball head handle rod 23 is first manually grasped and the right side cover plate 22 is opened upwards, and then the reagent bottle is placed inside the arc-shaped flexible clamp block 455 and pressed downwards, at which time the limit spring 453 will be stretched, and at the same time the clamp rod 454 will drive the arc-shaped flexible clamp block 455 to move outwards, and then the pressing of the reagent bottle is stopped, and the reaction force of the limit spring 453 will drive the arc-shaped flexible clamp block 455 to move inwards, thereby achieving the purpose of clamping the lower end of a single reagent bottle, and then the bottom fixing unit 45 is rotated to make the next reagent bottle placement station rotate to the previous placement area, and the above operation is repeated, so that each support plate 451 is placed with a reagent bottle and the lower end of the reagent bottle is initially clamped and fixed, and the right side cover plate 22 is closed, and then the upper right end of the reagent bottle storage box 2 is sealed; Start the electric push rod 46, and the electric push rod 46 drives the mounting column 471, the sliding rod 474 and the hollow pressure cap 475 to move downward, and the hollow pressure cap 475 will be sleeved on the outer side of the upper end of the reagent bottle, and then the electric push rod 46 will continue to move, and the preload spring 473 will be compressed, and the reaction force of the preload spring 473 will be further applied to the hollow pressure cap 475, so that the hollow pressure cap 475 applies a downward pressure to the reagent bottle, thereby pressing and fixing the reagent bottle, and the buffer protrusion 4511 of the hollow structure can be deformed adaptively, so as to avoid excessive pressure applied by the hollow pressure cap 475 and damage to the reagent bottle; During the downward movement, the mounting column 471 will also drive the L-shaped limit rod 476 to move downward, thereby passing through the cylindrical through hole and the limit hole in sequence, and the mounting column 471, the T-shaped slide 42 and the base 41 are fixed and limited by the L-shaped limit rod 476. At this time, the three will not be able to rotate at will, thereby ensuring the stability of the reagent bottle. The ball head handle rod 23 on the left is manually held and the cover plate 22 on the left is opened upward, and then the injection tube head 3 is inserted into the corresponding reagent bottle, the cover plate 22 on the left is closed, the high performance liquid chromatograph is powered on and the switch is turned on, and then the injection tube head 3 can pump the reagent in the reagent bottle into the chromatograph for detection, thereby obtaining the concentration data of various components in the reagent.

[0034] Embodiment 2:

[0035] The technical solution is basically the same as that of the first embodiment, see Figure 3 , Figure 4 , Figure 5 as well as Figure 8 The difference is that: a purification component 5 is arranged on the side of the reagent bottle fixing component 4, and the purification component 5 is fixedly installed on the inner wall of the reagent bottle storage box 2.

[0036] The purification component 5 includes a purification cylinder 51, which is fixedly installed on the inner wall of the reagent bottle storage box 2, a piston rod 52 is slidably installed inside the purification cylinder 51, and a purification plate 53 is fixedly installed below the piston rod 52. An air inlet pipe 54 is installed in the middle of the lower end of the purification cylinder 51, and a No. 1 one-way valve 55 is provided on the air inlet pipe 54. An air inlet hood 56 is installed at the end of the air inlet pipe 54. An air outlet pipe 57 is connected to the side wall of the purification cylinder 51 near the lower end, and the air outlet pipe 57 extends to the outside of the reagent bottle storage box 2 and a No. 2 one-way valve 58 is installed on the air outlet pipe 57.

[0037] The upper end of the piston rod 52 is fixedly connected with a linkage plate 521 , and the linkage plate 521 is connected to the telescopic end of the reciprocating cylinder 522 at a position away from the end of the piston rod 52 . The lower end of the reciprocating cylinder 522 is fixedly installed at the bottom of the reagent bottle storage box 2 .

[0038] During specific operation, if volatile gas components are detected during the reagent detection process, it means that volatile reagent components remain in the air inside the reagent bottle storage box 2. At this time, the reciprocating cylinder 522 is opened, and the piston rod 52 is driven by the reciprocating cylinder 522 to reciprocate up and down, thereby continuously sucking the volatile gas inside the reagent bottle storage box 2 into the purification cylinder 51, and then being absorbed and purified by the purification plate 53 and discharged through the outlet pipe 57; After the inspection of the current reagent bottle is completed, the reciprocating cylinder 522 stops moving, and the upper clamping unit 47 is driven by the electric push rod 46 to move upward, so that the lower end of the L-shaped limit rod 476 can be separated from the limit hole on the base 41. At this time, the left cover 22 is opened again manually, and the sampling tube head 3 is first pulled out from the reagent bottle that has been inspected, and then the bottom fixing unit 45 is rotated 60 degrees. The scale line set on the outer edge of the upper end surface of the annular skirt 43 facilitates manual faster and more accurate completion of the 60-degree rotation action. Subsequently, the L-shaped limit rod 476 is driven downward again by the electric push rod 46 to limit the base 41, and then the upper end of the sampling tube head 3 is reinserted into the new reagent bottle, and the above operations are repeated to complete the reagent component detection process in the subsequent reagent bottle.

[0039] It should be noted that during the 60-degree rotation of the bottom fixing unit 45, the hollow pressure cap 475 can still limit and fix the reagent bottle. In addition, after each reagent bottle conversion is completed, the test data within 5-10 seconds before the new test is not included in the experimental results. After each reagent bottle conversion is completed, the new reagent bottle should face the purification component 5. The purpose is to facilitate the insertion of the sample injection tube head 3 and the new reagent bottle.

[0040] The working principle of the present invention when in use: 1: Before the high performance liquid chromatograph is operated, first manually hold the ball-head handle rod 23 on the right side and open the cover plate 22 on the right side upwards, then place the reagent bottle inside the arc-shaped flexible clamp block 455 and press downwards, at this time, the limit spring 453 will be stretched, and at the same time, the clamp rod 454 will drive the arc-shaped flexible clamp block 455 to move outwards, then stop pressing the reagent bottle, and the reaction force of the limit spring 453 will drive the arc-shaped flexible clamp block 455 to move inwards, so as to achieve the purpose of clamping the lower end of a single reagent bottle, then rotate the bottom fixing unit 45 to make the next reagent bottle placement station rotate to the previous placement area, repeat the above operation, so that reagent bottles are placed on each support plate 451 and the lower ends of the reagent bottles are initially clamped and fixed, close the cover plate 22 on the right side, and then the upper right end of the reagent bottle storage box 2 is sealed; 2. After the above step 1 is completed, the electric push rod 46 is started, and the mounting column 471, the sliding rod 474 and the hollow pressure cap 475 are driven by the electric push rod 46 to move downward, and the hollow pressure cap 475 will be sleeved on the outer side of the upper end of the reagent bottle, and then the electric push rod 46 will continue to move, and the preload spring 473 will be compressed, and the reaction force of the preload spring 473 will be further applied to the hollow pressure cap 475, so that the hollow pressure cap 475 applies a downward pressure to the reagent bottle, thereby pressing and fixing the reagent bottle, and the buffer protrusion 4511 of the hollow structure can be deformed adaptively, so as to avoid excessive pressure applied by the hollow pressure cap 475 and damage the reagent bottle; 3: The mounting column 471 will also drive the L-shaped limiting rod 476 to move downward during the downward movement, thereby passing through the cylindrical through hole and the limiting hole in sequence, and the mounting column 471, the T-shaped slide 42 and the base 41 are fixed and limited by the L-shaped limiting rod 476. At this time, the three will not be able to rotate at will, thereby ensuring the stability of the reagent bottle. The ball head handle rod 23 on the left is manually held and the cover plate 22 on the left is opened upward, and then the injection tube head 3 is inserted into the corresponding reagent bottle, the cover plate 22 on the left is closed, the high performance liquid chromatograph is powered on and the switch is turned on, and then the injection tube head 3 can pump the reagent in the reagent bottle into the chromatograph for detection, thereby obtaining the concentration data of various components in the reagent; Fourth: During the reagent detection process, if volatile gas components are detected, it means that volatile reagent components remain in the air inside the reagent bottle storage box 2. At this time, the reciprocating cylinder 522 is opened, and the reciprocating cylinder 522 drives the piston rod 52 to reciprocate up and down, thereby continuously sucking the volatile gas inside the reagent bottle storage box 2 into the purification cylinder 51, and then being absorbed and purified by the purification plate 53 and discharged through the outlet pipe 57; 5: After the inspection of the current reagent bottle is completed, the reciprocating cylinder 522 stops moving, and the upper clamping unit 47 is driven by the electric push rod 46 to move upward, so that the lower end of the L-shaped limit rod 476 can be separated from the limit hole on the base 41. At this time, the left cover 22 is opened again manually, and the sampling tube head 3 is first pulled out from the reagent bottle that has been inspected, and then the bottom fixing unit 45 is rotated 60 degrees. The scale line set on the outer edge of the upper end surface of the annular skirt 43 facilitates manual faster and more accurate completion of the 60-degree rotation action. Subsequently, the L-shaped limit rod 476 is driven downward again by the electric push rod 46 to limit the base 41, and then the upper end of the sampling tube head 3 is reinserted into the new reagent bottle, and the above operations are repeated to complete the reagent component detection process in the subsequent reagent bottle.

[0041] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of the present invention is defined by the attached claims and their equivalents.

Claims

1. A high performance liquid chromatograph, comprising a chromatograph body (1), a reagent bottle storage box (2) being fixedly mounted on the upper end of the chromatograph body (1), a sample injection tube head (3) being mounted inside the chromatograph body (1), and an upper end of the sample injection tube head (3) extending into the reagent bottle storage box (2), characterized in that: A reagent bottle fixing assembly (4) is installed at the bottom of the reagent bottle storage box (2), a purification assembly (5) is arranged on the side of the reagent bottle fixing assembly (4), and the purification assembly (5) is fixedly installed on the inner wall of the reagent bottle storage box (2); The reagent bottle fixing assembly (4) comprises: A base (41), a base (41) is fixedly arranged in the middle of the bottom of the reagent bottle storage box (2), and a mounting groove is provided on the upper end surface of the base (41); A T-shaped slide (42), an inverted T-shaped slide (42) rotatably disposed in the mounting groove, and a cylindrical chamber is provided in the middle of the upper end of the T-shaped slide (42); An annular skirt (43), wherein an annular skirt (43) is fixedly provided at a position near the lower end of the edge of the T-shaped slide seat (42); L-shaped support rods (44), the side wall of the T-shaped slide seat (42) is located above the annular skirt (43) and is evenly installed with L-shaped support rods (44) along its circumference; A bottom fixing unit (45), the end of the L-shaped support rod (44) away from the T-shaped slide seat (42) is fixedly connected to the bottom fixing unit (45); An electric push rod (46) is fixedly installed in the cylindrical chamber; An upper end clamping unit (47) is fixedly connected to the upper end of the electric push rod (46).

2. A high performance liquid chromatograph according to claim 1, characterized in that: A fixing plate (21) is provided in the middle of the upper end of the reagent bottle storage box (2), and cover plates (22) are symmetrically hinged on both sides of the fixing plate (21). A ball-head handle rod (23) is fixedly mounted in the middle of the upper end surface of the cover plate (22) away from the fixing plate (21).

3. A high performance liquid chromatograph according to claim 1, characterized in that: The upper end surface of the base (41) is provided with limit holes evenly arranged along its circumference near the edge, and a cylindrical through hole is arranged above the limit hole. The annular skirt (43) and the L-shaped support rod (44) are penetrated by the cylindrical through hole, and the cylindrical through hole and the limit hole correspond to each other one by one.

4. A high performance liquid chromatograph according to claim 1, characterized in that: The outer edge of the upper end surface of the annular skirt (43) is provided with scale lines along its circumference.

5. A high performance liquid chromatograph according to claim 1, characterized in that: The bottom fixing unit (45) comprises a support plate (451), the end of the L-shaped support rod (44) away from the T-shaped slide seat (42) is fixedly connected to the support plate (451), the upper end surface of the support plate (451) is evenly provided with rectangular slide grooves (452) along its circumference, a clamping rod (454) is slidably installed in the rectangular slide groove (452) via a limit spring (453), and an arc-shaped flexible clamping block (455) is fixedly connected to the upper end of the clamping rod (454), and the concave arc surface of the arc-shaped flexible clamping block (455) is facing the middle of the support plate (451).

6. A high performance liquid chromatograph according to claim 5, characterized in that: A buffer protrusion (4511) is symmetrically arranged in the middle of the upper end surface of the support plate (451) and between the rectangular slide grooves (452), and the interior of the buffer protrusion (4511) is a hollow structure.

7. A high performance liquid chromatograph according to claim 5, characterized in that: The upper end surface of the arc-shaped flexible clamping block (455) is in a downwardly inclined arc surface structure near the concave arc surface, so that the bottom of the reagent bottle can separate the arc-shaped flexible clamping block (455).

8. A high performance liquid chromatograph according to claim 1, characterized in that: The upper end clamping unit (47) comprises a mounting column (471), the upper end of the electric push rod (46) is fixedly connected to the mounting column (471), the side wall of the mounting column (471) is evenly provided with a strip slide groove (472) along its circumference, a slide rod (474) is slidably arranged in the strip slide groove (472), a preload spring (473) is fixedly installed between the upper end of the slide rod (474) and the top of the strip slide groove (472), the end of the slide rod (474) away from the mounting column (471) is fixedly connected to a hollow pressure cap (475), and an L-shaped limit rod (476) is evenly installed on the side wall of the mounting column (471) near the lower end along its circumference, and the lower end of the L-shaped limit rod (476) is aligned with the columnar through hole and the limit hole.

9. A high performance liquid chromatograph according to claim 8, characterized in that: The purification component (5) comprises a purification cylinder (51), the purification cylinder (51) is fixedly mounted on the inner wall of the reagent bottle storage box (2), a piston rod (52) is slidably mounted inside the purification cylinder (51), a purification plate (53) is fixedly mounted below the piston rod (52), an air inlet pipe (54) is mounted in the middle of the lower end of the purification cylinder (51), a first check valve (55) is mounted on the air inlet pipe (54), an air inlet cover (56) is mounted at the end of the air inlet pipe (54), an air outlet pipe (57) is connected to the side wall of the purification cylinder (51) near the lower end, the air outlet pipe (57) extends to the outside of the reagent bottle storage box (2) and a second check valve (58) is mounted on the air outlet pipe (57).

10. A high performance liquid chromatograph according to claim 9, characterized in that: The upper end of the piston rod (52) is fixedly connected to a linkage plate (521), and a position of the linkage plate (521) away from the end of the piston rod (52) is connected to the telescopic end of the reciprocating cylinder (522), and the lower end of the reciprocating cylinder (522) is fixedly mounted on the bottom of the reagent bottle storage box (2).

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