Method for cultivating lasiosphaera seu calvatia by soil-rotten pine mycorrhizal fungi substitute and inoculation auxiliary device

Abstract

The invention discloses a method for substitute cultivation of puffball by soil-rotten pine mycorrhizal fungi and an inoculation auxiliary device, and relates to the technical field of puffball inoculation, the device comprises a fixed sleeve, a T-shaped push rod is slidably connected in the fixed sleeve in a penetrating mode, and the device further comprises an inoculation leakage-proof mechanism and an auxiliary matching mechanism. Real-time protection can be carried out when a fixed sleeve is not used for carrying out material taking and inoculation on puffball strains, a relatively closed space is formed at the material taking end of the fixed sleeve, microorganisms, dust and other pollutants in the external environment are effectively prevented from entering the container, and therefore the high purity and activity of the subsequently-taken strains are kept; and moreover, the material taking end of the fixed sleeve can be closed and covered after the fixed sleeve is used for taking materials, so that the situation that strains in the fixed sleeve fall off and leak out in the material taking, moving and inoculating process of an operator is effectively avoided, and the consistency of the number of the strains inoculated every time is ensured.

Description

Technical Field

[0001] The invention relates to the technical field of puffball inoculation, and in particular to a method for cultivating puffballs using soil-rotten pine wood mycorrhizal fungi as a substitute material and an inoculation auxiliary device. Background Art

[0002] In the prior art, the use of soil-rotten pine wood mycorrhizal fungi as a substitute for the cultivation of puffball inoculation refers to a technology that uses soil-rotten pine wood and the mycorrhizal fungi on it as a culture medium to replace the traditional culture medium to cultivate puffball. When used, it can improve the soil environment, increase soil fertility, and provide good nutritional conditions for the growth of puffball, thereby promoting its growth and development.

[0003] In the process of puffball cultivation, a related inoculator is usually needed, which is inserted into a container containing puffball fungi to extract the puffball fungi, and then the inoculator after extracting the material is moved to the fungus bag position, and the puffball fungi material taken from the inoculator is transported to the fungus bag to complete the inoculation of the puffball fungi. However, the existing inoculator structure is usually composed of a sleeve and a push rod, and the front end of the sleeve is used to extract the puffball fungi. Due to the single structure of the sleeve and the long exposure to the external environment, when the material is not extracted and inoculated, the sleeve in an exposed state is The material-taking end cannot prevent pollutants such as microorganisms and dust in the external environment from entering the inoculator, thereby contaminating the strains, reducing their purity and activity, and affecting the subsequent cultivation effect. At the same time, when the operator uses the sleeve to take materials and move the inoculation, due to the lack of a stable closed covering mechanism, the strains inside the sleeve are prone to falling or leaking, which not only leads to inconsistent numbers of strains inoculated each time, but also increases the risk of strain waste and contamination, thereby affecting the cultivation effect of puffball strains and reducing the success rate and yield of cultivation.

[0004] In view of this, the present invention proposes a method for cultivating puffballs using soil-rotten pine wood mycorrhizal fungi as a substitute material and an inoculation auxiliary device to make up for and improve the shortcomings of the prior art. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a method for cultivating puffballs using soil-rotten pine wood mycorrhizal fungi as a substitute and an inoculation auxiliary device to solve the corresponding technical problems raised in the above background technology.

[0006] To achieve the above purpose, the technical solution adopted by the present invention is: a method for inoculating puffballs with soil-rotten pine mycorrhizal fungi as a substitute for material cultivation, the steps of the inoculation method comprising:

[0007] Step 1: Prepare the inoculation environment and materials required for inoculation;

[0008] Step 2: Use an inoculator to inoculate the puffball strains;

[0009] Step 3: Seal the inoculated bags and carry out subsequent management.

[0010] The step 1 includes: first, ensuring that the inoculation room is strictly disinfected, preparing the inoculation gun, bacteria, disinfected bacteria bags and substitute materials, and the inoculation gun needs to be debugged in advance to ensure a large spraying area and sufficient pressure.

[0011] The step 2 comprises: after disinfecting the inoculation gun tip with 75% alcohol, inserting it into the bottom of the fungus bag, starting the inoculation gun, and spraying the fungus evenly from bottom to top until the fungus fills the bottom of the fungus bag, taking care to avoid spraying the fungus outside the bag to ensure a moderate inoculation amount.

[0012] The step three comprises: after the inoculation is completed, quickly seal the bag with a sponge block or a plastic cover to ensure that the tightness is appropriate and there is no leakage, move the bag to the culture room, control the temperature at 16-26°C, maintain a weak light or dark environment, observe the growth of the bag every day, and perform ventilation and humidity adjustment to keep the temperature constant.

[0013] The inoculation auxiliary device for the cultivation of puffballs with soil-rotten pine mycorrhizal fungi as a substitute material comprises a fixed sleeve, a T-shaped push rod is slidably connected to the inside of the fixed sleeve, and also comprises: an inoculation leak-proof mechanism and an auxiliary matching mechanism, and the inoculation leak-proof mechanism and the auxiliary matching mechanism are both arranged at one end of the fixed sleeve away from the T-shaped push rod;

[0014] The inoculation leak-proof mechanism is used to protect the fixed sleeve when it is not inoculated, and to close the end of the fixed sleeve away from the T-shaped push rod after the fixed sleeve is inoculated;

[0015] The auxiliary matching mechanism is used in conjunction with the inoculation leakage prevention mechanism.

[0016] Preferably, the inoculation leak-proof mechanism includes a first operating frame and a second operating frame which are slidably connected to the outer surface of the fixed sleeve, and the first operating frame and the second operating frame are fixedly connected, the outer surface of the first operating frame is slidably connected to a rectangular movable frame, and the outer surfaces of both sides of the first operating frame are symmetrically provided with sliding grooves, and sliding columns are slidably connected in the sliding grooves, and the end of the sliding column away from the first operating frame is fixedly connected to the rectangular movable frame.

[0017] Preferably, the inoculation leakage prevention mechanism also includes a half-frame protective cover symmetrically arranged on the end of the fixed sleeve away from the T-shaped push rod, the half-frame protective cover is symmetrically fixedly connected with a driven plate on the away side, the driven plate is fixedly connected with a first elastic telescopic column between the end facing the rectangular movable frame and the first operating frame, the half-frame protective cover is symmetrically fixedly connected with fixed columns on both sides, and a linkage plate is rotatably connected between the fixed column and the sliding column.

[0018] Preferably, an ear plate is symmetrically fixedly connected to the outer surface of one end of the second operating frame away from the first operating frame, a push plate is fixedly connected to the side of the fixed sleeve away from the first operating frame, and a second elastic telescopic column is symmetrically fixedly connected between the push plate and the ear plate.

[0019] Preferably, the auxiliary cooperation mechanism includes a fixed plate fixedly connected to the outer surface of the rectangular movable frame, a support column is fixedly connected to the outer surface of one end of the first operating frame away from the rectangular movable frame, an end of the support column away from the first operating frame is fixedly connected to a U-shaped positioning frame, an end of the fixed plate facing the U-shaped positioning frame is fixedly connected to a connecting column, and an end of the connecting column away from the fixed plate is fixedly connected to a limiting chuck.

[0020] Preferably, sliding rods are symmetrically penetrated and slidably connected on both sides of the U-shaped positioning frame, and the opposite ends of the sliding rods are fixedly connected with inclined blocks. The opposite sides of the inclined blocks facing the limit chuck are arranged with inclined surfaces, and the opposite sides of the inclined blocks away from the limit chuck are provided with limiting slots. A reset spring is fixedly connected between the inclined block and the U-shaped positioning frame, and the reset spring is sleeved on the outer surface of the sliding rod.

[0021] Preferably, the sliding rods are fixedly connected to movable plates at one end away from each other, wherein a pull rod is fixedly connected to one side of the movable plates away from the U-shaped positioning frame, support plates are symmetrically fixedly connected to both sides of the U-shaped positioning frame, a center axis is rotatably connected to the side of the support plate away from the first operating frame, a center plate is fixedly connected to the outer surface of one end of the center axis away from the support plate, and follower plates are symmetrically rotatably connected between the two ends of the center plate and the movable plate.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) Through the setting of the inoculation leak-proof mechanism, the two half-frame protective covers that are initially locked together can not only provide real-time protection when the fixed sleeve is not used to take materials for inoculation of puffball fungi, but also form a relatively closed space at the taking end of the fixed sleeve, effectively preventing microorganisms, dust and other pollutants in the external environment from entering the container, thereby maintaining the high purity and activity of the subsequent taking materials. In addition, the setting of the half-frame protective cover can also close and cover the taking end of the fixed sleeve after the fixed sleeve is used to take materials, thereby effectively preventing the fungi inside the fixed sleeve from falling or leaking during the process of the operator taking materials and moving for inoculation, ensuring the consistency of the number of fungi inoculated each time, making the cultivation process of puffball more stable and reliable, thereby improving the success rate and yield of cultivation.

[0024] (2) By setting up an auxiliary matching mechanism, utilizing the design of the inclined surface on the inclined block and the limit slot, and cooperating with the synchronous follow-up movement of the limit chuck, when the limit chuck moves, the inclined surface can provide a guiding function, so that the limit chuck can be stably moved to the limit chuck for limitation, thereby achieving effective limitation of the position of the rectangular moving frame after movement, avoiding the rectangular moving frame from loosening during the subsequent operation of the operator to retrieve the puffball species, causing the half-frame protective cover to reset and affecting the retrieval and inoculation of the puffball species. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of a preferred embodiment of the present invention;

[0026] Figure 2 It is a schematic diagram of the disassembled structure of the fixed sleeve and the T-shaped push rod shown in the present invention;

[0027] Figure 3 It is a schematic diagram of the structure of the connection of the rectangular movable frame shown in the present invention;

[0028] Figure 4 This is a schematic diagram of the structure of the connection of the half-frame protective cover shown in the present invention;

[0029] Figure 5 It is a structural schematic diagram of the connection between the first operating frame and the second operating frame shown in the present invention;

[0030] Figure 6 It is a schematic diagram of the structure of the U-shaped positioning frame connection shown in the present invention;

[0031] Figure 7 The present invention shows Figure 6 Schematic diagram of the method structure at A.

[0032] The numbers in the figure are:

[0033] 1. Fixed sleeve; 2. T-shaped push rod;

[0034] 3. Inoculation leak prevention mechanism; 301. First operating frame; 302. Second operating frame; 303. Slide; 304. Rectangular moving frame; 305. Sliding column; 306. Linkage plate; 307. Fixed column; 308. Half-frame protective cover; 309. Follower plate; 310. First elastic telescopic column; 311. Ear plate; 312. Second elastic telescopic column; 313. Push plate;

[0035] 4. Auxiliary matching mechanism; 401. Fixed plate; 402. Connecting column; 403. Limiting chuck; 404. Supporting column; 405. U-shaped positioning frame; 406. Sliding rod; 407. Return spring; 408. Bevel block; 409. Limiting slot; 410. Pull rod; 411. Moving plate; 412. Supporting plate; 413. Center axis; 414. Center plate; 415. Follower plate. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] Embodiment 1 of the present invention:

[0038] A method for inoculating puffballs with soil-rotten pine wood mycorrhizal fungi as a substitute for material cultivation, the inoculation method comprising the following steps:

[0039] Step 1: Prepare the inoculation environment and materials required for inoculation;

[0040] Step 2: Use an inoculator to inoculate the puffball strains;

[0041] Step 3: Seal the inoculated bags and conduct follow-up management.

[0042] Step one includes: first, ensure that the inoculation room has been strictly disinfected, prepare the inoculation gun, bacteria, disinfected bacteria bags and substitute materials (such as sawdust, wheat bran, etc.), and the inoculation gun needs to be debugged in advance to ensure a large spraying area and sufficient pressure.

[0043] Step 2 includes: disinfecting the inoculation gun tip with 75% alcohol, inserting it into the bottom of the bag, starting the inoculation gun, and spraying the bacteria evenly from bottom to top until the bottom of the bag is filled with bacteria. Be careful to avoid spraying the bacteria outside the bag to ensure that the inoculation amount is appropriate.

[0044] Step three includes: After the inoculation is completed, quickly seal the bag with a sponge or plastic cover to ensure that it is appropriately tight and there is no leakage. Move the bag to the culture room, control the temperature between 16 and 26 degrees Celsius, maintain a weak light or dark environment, observe the growth of the bag every day, and adjust ventilation and humidity to keep the temperature constant.

[0045] Embodiment 2 of the present invention:

[0046] Please refer to Figures 1 to 7As shown, the soil-rotten pine wood mycorrhizal fungus substitute material cultivation puffball inoculation auxiliary device comprises a fixed sleeve 1, the interior of the fixed sleeve 1 is penetrated by a T-shaped push rod 2 which is slidably connected, and also comprises: an inoculation leak-proof mechanism 3 and an auxiliary matching mechanism 4, and the inoculation leak-proof mechanism 3 and the auxiliary matching mechanism 4 are both arranged at one end of the fixed sleeve 1 away from the T-shaped push rod 2;

[0047] The inoculation leak-proof mechanism 3 is used to protect the fixed sleeve 1 when it is not inoculated, and to close the end of the fixed sleeve 1 away from the T-shaped push rod 2 after the fixed sleeve 1 is inoculated;

[0048] The auxiliary matching mechanism 4 is used in conjunction with the inoculation leak prevention mechanism 3;

[0049] The inoculation leak-proof mechanism 3 includes a first operating frame 301 and a second operating frame 302 which penetrate and slide to the outer surface of the fixed sleeve 1, and the first operating frame 301 and the second operating frame 302 are fixedly connected, the outer surface of the first operating frame 301 is slidably connected to a rectangular movable frame 304, and the outer surfaces of both sides of the first operating frame 301 are symmetrically provided with sliding grooves 303, and sliding columns 305 are slidably connected in the sliding grooves 303, and one end of the sliding column 305 away from the first operating frame 301 is fixedly connected to the rectangular movable frame 304;

[0050] The inoculation leak-proof mechanism 3 also includes a half-frame protective cover 308 symmetrically arranged at one end of the fixed sleeve 1 away from the T-shaped push rod 2, a driven plate 309 is symmetrically fixedly connected to the side away from the half-frame protective cover 308, a first elastic telescopic column 310 is fixedly connected between the end of the driven plate 309 facing the rectangular movable frame 304 and the first operating frame 301, fixed columns 307 are symmetrically fixedly connected to both sides of the half-frame protective cover 308, and a linkage plate 306 is rotatably connected between the fixed column 307 and the sliding column 305;

[0051] An ear plate 311 is symmetrically fixedly connected to the outer surface of one end of the second operating frame 302 away from the first operating frame 301 , a push plate 313 is fixedly connected to the side of the fixed sleeve 1 away from the first operating frame 301 , and a second elastic telescopic column 312 is symmetrically fixedly connected between the push plate 313 and the ear plate 311 .

[0052] The effects achieved by this embodiment are as follows: compared with the prior art, through the setting of the inoculation leak-proof mechanism 3, by using the two half-frame protective covers 308 that are initially locked with each other, not only can real-time protection be provided when the fixed sleeve 1 is not used to take materials for inoculation of the puffball strains, so that the material taking end of the fixed sleeve 1 forms a relatively closed space, effectively preventing microorganisms, dust and other pollutants in the external environment from entering the interior of the container, thereby maintaining the high purity and activity of the subsequent material taking strains, and the setting of the half-frame protective cover 308 can also close and cover the material taking end of the fixed sleeve 1 after the fixed sleeve 1 is used to take materials, thereby effectively preventing the strains inside the fixed sleeve 1 from falling or leaking during the process of the operator taking materials and moving for inoculation, ensuring the consistency of the number of strains inoculated each time, making the puffball cultivation process more stable and reliable, thereby improving the success rate and yield of the cultivation.

[0053] Further examples:

[0054] Please refer to Figures 1 to 7 As shown, the auxiliary matching mechanism 4 includes a fixed plate 401 fixedly connected to the outer surface of the rectangular moving frame 304, a support column 404 is fixedly connected to the outer surface of one end of the first operating frame 301 away from the rectangular moving frame 304, and a U-shaped positioning frame 405 is fixedly connected to the end of the support column 404 away from the first operating frame 301, and a connecting column 402 is fixedly connected to the end of the fixed plate 401 facing the U-shaped positioning frame 405, and a limited position chuck 403 is fixedly connected to the end of the connecting column 402 away from the fixed plate 401;

[0055] The U-shaped positioning frame 405 is symmetrically penetrated by sliding rods 406 for sliding connection on both sides, and the opposite ends of the sliding rods 406 are fixedly connected with inclined blocks 408, and the opposite sides of the inclined blocks 408 facing the limiting chuck 403 are all set as inclined surfaces, and the opposite sides of the inclined blocks 408 away from the limiting chuck 403 are provided with limiting grooves 409, and a return spring 407 is fixedly connected between the inclined block 408 and the U-shaped positioning frame 405, and the return spring 407 is sleeved on the outer surface of the sliding rod 406;

[0056] The sliding rod 406 is fixedly connected to a moving plate 411 at one end away from the other, wherein a pull rod 410 is fixedly connected to one side of the moving plate 411 away from the U-shaped positioning frame 405, and support plates 412 are symmetrically fixedly connected to both sides of the U-shaped positioning frame 405, and a central axis 413 is rotatably connected to the side of the support plate 412 away from the first operating frame 301, and a central plate 414 is fixedly connected to the outer surface of one end of the central axis 413 away from the support plate 412, and follower plates 415 are symmetrically rotatably connected between the two ends of the central plate 414 and the moving plate 411.

[0057] The effects achieved by this embodiment are as follows: compared with the prior art, through the setting of the auxiliary matching mechanism 4, utilizing the design of the inclined surface on the inclined block 408 and the limiting slot 409, and cooperating with the synchronous following movement of the limiting chuck 403, when the limiting chuck 403 moves, the inclined surface can provide a guiding function, so that the limiting chuck 403 can be stably moved to the limiting chuck 403 for limitation, thereby achieving effective limitation of the position of the rectangular moving frame 304 after movement, avoiding the rectangular moving frame 304 from loosening during the subsequent operator's collection of puffball species, causing the half-frame protective cover 308 to reset and affecting the collection and inoculation of the puffball species.

[0058] The complete usage steps and working principle of the above embodiment are as follows:

[0059] In the initial state, the T-shaped push rod 2 is slidably set on the fixed sleeve 1, and when the puffball fungus is not taken, the T-shaped push rod 2 is at the rear end of the fixed sleeve 1, and the front end of the fixed sleeve 1 is used to take the puffball fungus. When taking the material, the operator can insert the fixed sleeve 1 into the container containing the fungus to take the fungus. After the taking action is completed, the fixed sleeve 1 can be inserted into the fungus bag, and the material taken at the front end of the fixed sleeve 1 can be pushed out by pushing the T-shaped push rod 2, so that the fungus enters the fungus bag, thereby completing the taking and inoculation of the puffball fungus.

[0060] Before inserting the fixed sleeve 1 into the container containing the bacterial strains to extract the bacterial strains, Figures 1 to 4 As shown, since the first operating frame 301 is slidably provided on the outer surface of the fixed sleeve 1, and the rectangular moving frame 304 is slidably provided on the outer surface of the first operating frame 301, and the outer surface of the first operating frame 301 is symmetrically provided with a slide groove 303, a slide post 305 is slidably provided in the slide groove 303, and the slide post 305 and the rectangular moving frame 304 are fixedly connected, when in use, the operator can control the first operating frame 301, and based on the first operating frame 301, push the rectangular moving frame 304 on the first operating frame 301 to make the rectangular moving frame 304 move. The T-shaped moving frame 304 moves toward the front end of the fixed sleeve 1 (i.e., the end away from the T-shaped push rod 2), and the sliding column 305 will move synchronously in the sliding groove 303. In addition, because the front end of the fixed sleeve 1 is symmetrically provided with two half-frame protective covers 308, the two half-frame protective covers 308 are initially connected to each other and cover the front end of the fixed sleeve 1, so as to protect the front end of the fixed sleeve 1 (i.e., the material collection and inoculation end) when not in use, so as to avoid damage to the front end of the fixed sleeve 1 and affect the subsequent collection and inoculation of puffball bacteria. Figure 3 and Figure 4As shown, a driven plate 309 is fixedly arranged on the side away from the two half-frame protective covers 308, and a first elastic telescopic column 310 is fixedly arranged on the driven plate 309 at one end facing the rectangular movable frame 304 and the outer wall of the first operating frame 301, and a fixed column 307 is symmetrically fixedly arranged on the half-frame protective cover 308, and a linkage plate 306 is rotatably arranged between the fixed column 307 and the sliding column 305. Therefore, when the operator pushes the rectangular movable frame 304 toward the half-frame protective cover 308 to make the sliding column 305 slide synchronously on the outer wall of the first operating frame 301, the linkage plate 306 is synchronously rotated by the push of the sliding column 305, and is driven by the fixed column 307 arranged at one end away from the sliding column 305, so that the two The half-frame protective cover 308 moves in the opposite direction therebetween. It should be noted that the two linkage plates 306 initially form a V-shape. When the sliding column 305 pushes the linkage plate 306, the two linkage plates 306 can be driven to open outward, thereby opening the two half-frame protective covers 308, thereby releasing the clamping relationship between the two half-frame protective covers 308 and increasing the distance between the two half-frame protective covers 308 to facilitate the subsequent passage of the fixed sleeve 1. At the same time, since the half-frame protective cover 308 is connected to the first operating frame 301 through the driven plate 309 and the first elastic telescopic column 310, when the half-frame protective cover 308 is driven to move outward, the driven plate 309 moves outward synchronously and drives the first elastic telescopic column 310 to stretch, such as Figures 4 to 6As shown, since a fixed plate 401 is fixedly provided on the rectangular movable frame 304, and the fixed plate 401 is fixedly connected to the limiting chuck 403 on one side facing the half-frame protective cover 308 through a connecting column 402, and a support column 404 is fixedly provided on the outer surface of one end of the first operating frame 301 facing the half-frame protective cover 308 in the same direction as the fixed plate 401, and a U-shaped positioning frame 405 is fixedly provided on the support column 404. When the operator pushes the rectangular movable frame 304 in the direction of the half-frame protective cover 308, the position of the fixed plate 401 is synchronously changed, and the limiting chuck 403 is synchronously driven to be pushed in the direction of the U-shaped positioning frame 405. Since sliding rods 406 are symmetrically provided on both sides of the U-shaped positioning frame 405 for sliding, inclined blocks 408 are fixedly provided on the opposite ends of the sliding rods 406, and initially, the opposite surfaces of the two inclined blocks 408 facing one end of the rectangular movable frame 304 are both set as inclined surfaces, and the inclined surfaces are The spacing between them gradually decreases from one end facing the rectangular moving frame 304 to the end away from the rectangular moving frame 304. As the rectangular moving frame 304 continues to move, the limiting chuck 403 will enter between the two inclined blocks 408, and the outer surface of the limiting chuck 403 will contact the facing surfaces of the two inclined blocks 408. After the limiting chuck 403 continues to be pushed, under the action of the inclined surface, the two inclined blocks 408 will be moved in opposite directions between them, and the sliding rod 406 will be moved synchronously at the two ends of the U-shaped positioning frame 405. Because a return spring 407 is fixedly arranged between the U-shaped positioning frame 405 and the inclined block 408, and the return spring 407 is sleeved on the outer surface of the sliding rod 406, when the inclined block 408 is pushed outward by the limiting chuck 403 and drives the sliding rod 406 to move synchronously through the U-shaped positioning frame 405, the return spring 407 is compressed synchronously. Figure 6 As shown, because the two inclined blocks 408 are both provided with limiting slots 409 on the opposite sides of one end away from the rectangular moving frame 304, as the limiting chuck 403 continues to move, when the limiting chuck 403 completely passes through the inclined surface, it will enter between the limiting slots 409, and the outward thrust generated by the limiting chuck 403 on the inclined block 408 disappears. Under the elastic force of the reset spring 407, the two inclined blocks 408 are moved toward the middle for reset, and the limiting chuck 403 is also synchronously stuck in the limiting slots 409. The setting of the limiting slots 409 is utilized to limit the position of the limiting chuck 403, thereby realizing the position limitation of the rectangular moving frame 304 after movement, avoiding the rectangular moving frame 304 from loosening in the subsequent material taking process, causing the half-frame protective cover 308 to reset and affecting the material taking and inoculation of the puffball strains;

[0061] After the operator opens the two half-frame protective covers 308 by moving the rectangular moving frame 304 and limits the limit chuck 403 by using the design of the limit card slot 409, and simultaneously limits the position of the half-frame protective covers 308 after opening, the operator can continue to take the first operating frame 301, such as Figure 1 and Figure 2 As shown, since the first operating frame 301 and the second operating frame 302 are fixedly connected, the ear plate 311 is symmetrically fixedly arranged on the second operating frame 302, the rear end of the fixed sleeve 1 (i.e., the end away from the semi-frame protective cover 308) is fixedly connected to the push plate 313, and the second elastic telescopic column 312 is symmetrically fixedly arranged between the push plate 313 and the ear plate 311. The operator can continue to push the push plate 313 toward the container containing the puffball strains based on the first operating frame 301 and the second operating frame 302, so that the second elastic telescopic column 312 is compressed, and the front end of the fixed sleeve 1 can be inserted into the container to extract the puffball strains in the container. After the extraction is completed, the pushing control of the push plate 313 can be released, so that the push plate 313 moves in the direction away from the second operating frame 302 under the elastic force of the second elastic telescopic column 312, and the position of the fixed sleeve 1 is restored. The design of the second elastic telescopic column 312 makes it easy for the fixed sleeve 1 to be inserted into and out of the container, as shown in FIG. Figure 6 and Figure 7As shown, since one end of the sliding rod 406 away from the inclined block 408 is fixedly connected to the moving plate 411, and a pull rod 410 is fixedly provided on one of the moving plates 411, and support plates 412 are symmetrically fixedly provided on both sides of the U-shaped positioning frame 405, a central axis 413 is rotatably provided on the support plate 412, a central plate 414 is fixedly provided on the outer surface of the central axis 413, and a follower plate 415 is symmetrically rotatably provided between the central plate 414 and the moving plate 411, after the fixed sleeve 1 finishes the material taking action and resets, the operator can pull the pull rod 410 outward to make the moving plate 411 move synchronously, and under the connection action of the follower plate 415, the central plate 414 can be synchronously driven to rotate with the central axis 413 as the axis, thereby driving the other follower plate 415 to move, and the other moving plate 411 is synchronously pushed outward, at this time, the two sliding The rod 406 slides outward synchronously, and can pull the inclined block 408 to move synchronously, thereby increasing the distance between the two inclined blocks 408. Through the elastic force of the first elastic telescopic column 310 mentioned above, the two half-frame protective covers 308 can be pushed in the directions facing each other, and under the connection of the linkage plate 306, the rectangular movable frame 304 is synchronously driven to return to the initial position. As the rectangular movable frame 304 moves, the limit chuck 403 is synchronously separated from the limit slot 409. At this time, the two half-frame protective covers 308 will re-form a closed clamping relationship, and the front end of the fixed sleeve 1 will be covered and covered, so as to achieve the blocking of the material taking port of the fixed sleeve 1, and avoid the situation that the bacteria taken by the fixed sleeve 1 falls or leaks during the movement of the operator, resulting in different numbers of bacteria inoculated each time, which affects the cultivation of puffballs.

[0062] The circuits and controls involved in the present invention are all prior art and will not be described in detail here.

[0063] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for inoculating puffballs with soil-rotten pine mycorrhizal fungi as a substitute for material cultivation, characterized in that: The steps of the inoculation method include: Step 1: Prepare the inoculation environment and materials required for inoculation; Step 2: Use an inoculator to inoculate the puffball strains; Step 3: Seal the inoculated bags and carry out subsequent management.

2. A method for inoculating puffballs with soil-rotten pine mycorrhizal fungi as a substitute for material cultivation according to claim 1, characterized in that: The step 1 comprises: first, disinfecting the inoculation room, preparing an inoculation gun, bacteria, disinfected bacteria bags and substitute materials; The second step comprises: after disinfecting the inoculation gun tip with 75% alcohol, inserting it into the bottom of the bacteria bag, starting the inoculation gun, and spraying the bacteria evenly from bottom to top.

3. A method for inoculating puffballs with soil-rotten pine mycorrhizal fungi as a substitute for material cultivation according to claim 2, characterized in that: The step three includes: after the inoculation is completed, the fungus bag is sealed and moved to the culture room, the temperature of the culture room is adjusted to 16-26°C to maintain the growth environment of the fungus, the growth of the fungus bag is observed every day, and ventilation and humidity are adjusted to keep the temperature constant.

4. An auxiliary device for inoculating puffballs with soil-rotten pine mycorrhizal fungi as a substitute for material cultivation, applied to a method for inoculating puffballs with soil-rotten pine mycorrhizal fungi as a substitute for material cultivation as claimed in any one of claims 1 to 3, comprising a fixed sleeve (1), wherein a T-shaped push rod (2) is slidably connected to the inside of the fixed sleeve (1), characterized in that: It also includes: an inoculation leak-proof mechanism (3) and an auxiliary matching mechanism (4), and the inoculation leak-proof mechanism (3) and the auxiliary matching mechanism (4) are both arranged at one end of the fixed sleeve (1) away from the T-shaped push rod (2); The inoculation leak-proof mechanism (3) is used to provide protection when the fixed sleeve (1) is not inoculated, and to close the end of the fixed sleeve (1) away from the T-shaped push rod (2) after the fixed sleeve (1) is inoculated; The auxiliary matching mechanism (4) is used in conjunction with the inoculation leakage prevention mechanism (3).

5. The soil-rotten pine mycorrhizal fungus substitute material cultivation puffball inoculation auxiliary device according to claim 4, characterized in that: The inoculation leak prevention mechanism (3) includes a first operating frame (301) and a second operating frame (302) which are slidably connected to the outer surface of the fixed sleeve (1), and the first operating frame (301) and the second operating frame (302) are fixedly connected, the outer surface of the first operating frame (301) is slidably connected to a rectangular movable frame (304), and the outer surfaces of both sides of the first operating frame (301) are symmetrically provided with sliding grooves (303), and a sliding column (305) is slidably connected in the sliding groove (303), and the sliding column (305) is fixedly connected to the rectangular movable frame (304) at one end away from the first operating frame (301).

6. The soil-rotten pine mycorrhizal fungus substitute material cultivation puffball inoculation auxiliary device according to claim 5, characterized in that: The inoculation leak prevention mechanism (3) also includes a semi-frame protective cover (308) symmetrically arranged on one end of the fixed sleeve (1) away from the T-shaped push rod (2), and a driven plate (309) is symmetrically fixedly connected to the side away from the semi-frame protective cover (308), and a first elastic telescopic column (310) is fixedly connected between the end of the driven plate (309) facing the rectangular movable frame (304) and the first operating frame (301), and fixed columns (307) are symmetrically fixedly connected on both sides of the semi-frame protective cover (308), and a linkage plate (306) is rotatably connected between the fixed column (307) and the sliding column (305).

7. The soil-rotten pine wood mycorrhizal fungus substitute material cultivation puffball inoculation auxiliary device according to claim 5, characterized in that: An ear plate (311) is symmetrically fixedly connected to the outer surface of one end of the second operating frame (302) away from the first operating frame (301), a push plate (313) is fixedly connected to the side of the fixed sleeve (1) away from the first operating frame (301), and a second elastic telescopic column (312) is symmetrically fixedly connected between the push plate (313) and the ear plate (311).

8. The soil-rotten pine mycorrhizal fungus substitute material cultivation puffball inoculation auxiliary device according to claim 5, characterized in that: The auxiliary matching mechanism (4) comprises a fixing plate (401) fixedly connected to the outer surface of the rectangular movable frame (304); a support column (404) is fixedly connected to the outer surface of one end of the first operating frame (301) away from the rectangular movable frame (304); an end of the support column (404) away from the first operating frame (301) is fixedly connected to a U-shaped positioning frame (405); an end of the fixing plate (401) facing the U-shaped positioning frame (405) is fixedly connected to a connecting column (402); and an end of the connecting column (402) away from the fixing plate (401) is fixedly connected to a limiting chuck (403).

9. The soil-rotten pine wood mycorrhizal fungus substitute material cultivation puffball inoculation auxiliary device according to claim 8, characterized in that: The U-shaped positioning frame (405) has sliding rods (406) symmetrically penetrated and slidably connected on both sides, and the opposite ends of the sliding rods (406) are fixedly connected with inclined blocks (408), and the opposite side of the inclined block (408) facing the limiting chuck (403) is set as an inclined surface, and the opposite side of the inclined block (408) away from the limiting chuck (403) is provided with a limiting groove (409), and a reset spring (407) is fixedly connected between the inclined block (408) and the U-shaped positioning frame (405), and the reset spring (407) is sleeved on the outer surface of the sliding rod (406).

10. The soil-rotten pine wood mycorrhizal fungus substitute material cultivation puffball inoculation auxiliary device according to claim 9, characterized in that: The sliding rod (406) is fixedly connected to a moving plate (411) at one end away from the other end, and a pull rod (410) is fixedly connected to one side of the moving plate (411) away from the U-shaped positioning frame (405), and support plates (412) are symmetrically fixedly connected to both sides of the U-shaped positioning frame (405), and the support plate (412) is rotatably connected to the side away from the first operating frame (301) The center axis (413) is fixedly connected to the outer surface of one end away from the support plate (412) The center plate (414) is fixedly connected to the outer surface of one end of the center axis (413) away from the support plate (412), and follower plates (415) are symmetrically rotatably connected between the two ends of the center plate (414) and the moving plate (411).

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