HDA isothermal amplification kit for detecting bursaphelenchus xylophilus

Abstract

The invention relates to the technical field of isothermal amplification kits, and discloses an HDA isothermal amplification kit for detecting bursaphelenchus xylophilus, which comprises a kit body, a base plate fixedly mounted at the bottom of the kit body, and a storage assembly arranged at the side position of the kit body, the storage assembly comprises an adjusting mechanism arranged at the position of the inner wall of the kit body, a limiting mechanism is installed at the position of the right side wall of the kit body, an auxiliary mechanism is arranged at the position of the inner wall of the kit body and connected with one end of the adjusting mechanism, and the adjusting mechanism comprises a mounting frame; the mounting frame is fixedly mounted on the inner wall of the kit body. The device solves the problems that the positions of the kits are adjusted from left to right by rotating a screw rod, but the number of the kits is effective, and the internal space of the kits is difficult to fully utilize, so that certain limitation exists when the device is used.

Description

Technical Field

[0001] The invention relates to the technical field of isothermal amplification kits, in particular to a HDA isothermal amplification kit for detecting pine wood nematodes. Background Art

[0002] Pine wood nematodes are common pine parasites. The adult body is about 1 mm long. The tail of the female is nearly conical with a rounded end. The tail of the male is like a bird's claw and bends toward the ventral side. It is transmitted by vector insects such as Monochamus alternatus, thus causing pine wood nematode disease. The needles of the infected pine trees are yellow-brown or reddish-brown, and the whole tree dries up and dies, and eventually rots. The test kit is a box for containing chemical reagents such as chemical components, drug residues, and virus types. It is generally used in hospitals and pharmaceutical companies. Therefore, it is necessary to use an HDA isothermal amplification kit for detecting pine wood nematodes;

[0003] For example, Chinese patent CN213951200U discloses an HDA isothermal amplification kit for detecting pine wood nematodes, wherein a threaded rod is arranged inside the kit body, a sliding rod is arranged inside the kit body, a slider is arranged on the side surface of the sliding rod, a threaded block is arranged on the side surface of the threaded rod, a heating box is arranged on the top of the threaded block, the left side of the kit body is sealed, and a groove is opened on the right side. Reagents that are not needed are located on the left side of the kit body, and used reagents are located inside the groove on the right side of the kit body, which can prevent accidents when taking them out, thereby protecting the reagents;

[0004] However, there are certain defects in this patent. The device adjusts the position of the reagent box from left to right by rotating the screw, but the number of reagent boxes is limited and it is difficult to fully utilize its internal space, which leads to certain limitations when the device is used. Summary of the invention

[0005] The purpose of the present invention is to provide a HDA isothermal amplification kit for detecting pine wood nematodes, so as to achieve the purpose of solving the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solution: an HDA isothermal amplification kit for detecting pine wood nematodes, comprising a kit body,

[0007] A pad fixedly mounted on the bottom of the reagent kit body;

[0008] and a storage component arranged at the side of the reagent box body;

[0009] The storage assembly includes an adjustment mechanism disposed on the inner wall of the reagent box body;

[0010] A limiting mechanism is installed on the right side wall of the reagent box body;

[0011] An auxiliary mechanism is arranged on the inner wall of the reagent box body, and the auxiliary mechanism is connected to one end of the regulating mechanism.

[0012] Preferably, a protective cover is hingedly mounted on the top side wall of the reagent box body via a hinge, the bottom of the protective cover is arranged in contact with the top of the reagent box body, and a handle is fixedly mounted on the top of the protective cover.

[0013] Preferably, the adjusting mechanism comprises a mounting frame, the mounting frame is fixedly mounted on the inner wall of the reagent box body, the inner wall of the mounting frame is slidably mounted with a sliding column 2, the top of the sliding column 2 is fixedly mounted with a heating box 2, the bottom of the heating box 2 is slidably connected to the top of the mounting frame, the side wall of the sliding column 2 is contacted with an adjusting rod, one end of the adjusting rod is fixedly mounted with a rectangular block, the inner wall of the rectangular block is mounted with a screw 1, one end of the screw 1 movably penetrates the inner wall of the reagent box body and extends to the outside of the reagent box body, and a rotating disk 1 is transmission-mounted, the inner wall of the mounting frame is slidably mounted with a sliding column 1, the top of the sliding column 1 is fixedly mounted with a heating box 1, the bottom of the heating box 1 is slidably connected to the top of the mounting frame, the inner wall of the sliding column 1 is mounted with a screw 2, one end of the screw 2 movably penetrates the inner wall of the reagent box body and extends to the outside of the reagent box body, and a rotating disk 2 is transmission-mounted.

[0014] Preferably, the bottom of the rectangular block is slidably connected to the inner wall of the reagent box body, the side wall of the screw rod 1 is threadedly connected to the inner wall of the rectangular block, and the inner wall of the sliding column 1 is threadedly connected to the side wall of the screw rod 2.

[0015] Preferably, the limiting mechanism includes a mounting plate, the mounting plate is fixedly mounted on the right side wall of the test kit body, a plug-in rod is slidably mounted on the inner wall of the mounting plate, a pull plate is fixedly mounted on the bottom of the plug-in rod, a limiting plate is slidably mounted on the top of the plug-in rod, the side wall of the limiting plate is fixedly connected to the side wall of the protective cover, a connecting spring is fixedly mounted on the bottom of the mounting plate, and the bottom of the connecting spring is fixedly connected to the top of the pull plate.

[0016] Preferably, the diameter of the side wall of the plug-in rod is the same as the inner wall diameter of the running groove provided on the inner wall of the limiting plate, and the side wall of the plug-in rod is slidably connected to the inner wall of the circular groove.

[0017] Preferably, the auxiliary mechanism includes a compression spring and a limit rod, one end of the compression spring is fixedly connected to the inner wall of the mounting frame, the other end of the compression spring is fixedly installed with a displacement plate, the side wall of the displacement plate is slidably connected to the inner wall of the mounting frame, the limit rod is fixedly connected to the inner wall of the heating box, and the side wall of the limit rod is slidably installed with a partition.

[0018] Preferably, there are four pads, the four pads are equal in shape and size, and the four pads are respectively fixedly mounted at the four corners of the bottom of the reagent kit body.

[0019] The present invention provides a HDA isothermal amplification kit for detecting pine wood nematodes. It has the following beneficial effects:

[0020] (1) According to the present invention, the operator can rotate the rotating disk 2 and the rotating disk 1 separately as needed. When the rotating disk 2 is rotated, the rotating disk 2 will drive the position of the screw rod 2 to rotate. Since the side wall of the screw rod 2 is threadedly connected to the inner wall of the sliding column 1, the screw rod 2 can effectively drive the sliding column 1 and the heating box 1 to slide on the inner wall of the installation frame when the screw rod 2 is rotated, so as to move them to the right side of the installation frame. When the operator rotates the position of the rotating disk 1, the rotating disk 1 drives the position of the screw rod 1 to rotate. Since the side wall of the screw rod 1 is threadedly connected to the inner wall of the rectangular block, the screw rod 1 can drive the rectangular block to the right side when the screw rod 1 is rotated. Under the action of the adjusting rod, the rectangular block drives the sliding column 2 and the heating box 2 to move. The heating box 2 and the sliding column 2 slide from left to right on the installation frame, which is convenient for the operator to use the heating box 2. The problem that the existing device adjusts the position of the reagent kit from left to right by rotating the screw rod is solved, but the number of reagent kits is limited and it is difficult to make full use of the internal space thereof, resulting in certain limitations when the device is used.

[0021] (2) When the operator needs to use the heating box and open the protective cover, the operator can directly pull down the pull plate, which drives the plug-in rod to slide on the inner wall of the limit plate. The pull plate will also pull one end of the connecting spring. When one end of the plug-in rod is separated from the inner wall of the limit plate, the operator can pull the handle, and the handle will drive the protective cover to rotate on the top of the reagent box body. The position of the protective cover can be effectively limited by the limiting mechanism.

[0022] (3) The present invention can use the displacement plate and the compression spring to enable the sliding column 2 to effectively slide to its original position when it is not squeezed by the adjustment rod. By designing a partition on the side wall of the limit rod, different reagents can be effectively separated and stored, which is convenient for operators to better use the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the appearance structure of the present invention;

[0024] Figure 2 It is a schematic diagram of the side structure of the present invention;

[0025] Figure 3 It is a schematic diagram of a top-view cross-sectional structure of the present invention;

[0026] Figure 4 It is a structural schematic diagram of the regulating mechanism of the present invention;

[0027] Figure 5 For the present invention Figure 1 A partial enlarged view of middle A.

[0028] In the figure: 1. reagent box body; 2. pad; 3. protective cover; 4. storage assembly; 41. adjustment mechanism; 411. mounting frame; 412. screw rod 1; 413. rotating disk 1; 414. rotating disk 2; 415. rectangular block; 416. adjustment rod; 417. sliding column 1; 418. screw rod 2; 419. heating box 1; 4110. heating box 2; 4111. sliding column 2; 42. limiting mechanism; 421. limiting plate; 422. plug-in rod; 423. mounting plate; 424. pulling plate; 425. connecting spring; 43. auxiliary mechanism; 431. compression spring; 432. displacement plate; 433. limiting rod; 434. partition; 5. handle. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0030] Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0031] In the description of the present invention, it is to be understood that the terms “center”, “longitudinal”, “lateral”, “length”, “width”, “thickness”, “up”, “down”, “front”, “back”, “left”, “right”, “vertical”, “horizontal”, “top”, “bottom”, “inside”, “outside”, “clockwise”, “counterclockwise”, “axial”, “radial”, “circumferential”, etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0032] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0033] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, specific embodiments of the present invention are now described with reference to the accompanying drawings.

[0034] Example 1: A preferred embodiment of the HDA isothermal amplification kit for detecting pine wood nematodes provided by the present invention is as follows: Figures 1 to 5 As shown: an HDA isothermal amplification kit for detecting pine wood nematodes, comprising a kit body 1, a protective cover 3 is hingedly mounted on the top side wall of the kit body 1 through a hinge, the bottom of the protective cover 3 is arranged in contact with the top of the kit body 1, and a handle 5 is fixedly mounted on the top of the protective cover 3, and an operator can open the position of the protective cover 3 through the handle.

[0035] A pad 2 is fixedly mounted on the bottom of the reagent box body 1. There are four pads 2. The four pads 2 are of equal shape and size. The four pads 2 are respectively fixedly mounted at the four corners of the bottom of the reagent box body 1.

[0036] and a storage component 4 disposed at the side of the reagent box body 1;

[0037] The storage assembly 4 includes an adjustment mechanism 41 disposed on the inner wall of the reagent box body 1;

[0038] A limit mechanism 42 is installed on the right side wall of the reagent box body 1;

[0039] An auxiliary mechanism 43 is disposed on the inner wall of the reagent box body 1 , and the auxiliary mechanism 43 is connected to one end of the adjustment mechanism 41 .

[0040] The adjustment mechanism 41 includes a mounting frame 411, which is fixedly mounted on the inner wall of the reagent box body 1, a sliding column 4111 is slidably mounted on the inner wall of the mounting frame 411, a heating box 4110 is fixedly mounted on the top of the sliding column 4111, and the bottom of the heating box 4110 is slidably connected to the top of the mounting frame 411, and an adjustment rod 416 is provided in contact with the side wall of the sliding column 4111, and a rectangular block 415 is fixedly mounted on one end of the adjustment rod 416, and a screw 412 is mounted on the inner wall of the rectangular block 415, and the screw 412 One end of the reagent box 418 is movably penetrated through the inner wall of the reagent box body 1 and extends to the outside of the reagent box body 1, and a rotating disk 413 is installed for transmission. A sliding column 417 is slidably installed on the inner wall of the mounting frame 411. A heating box 419 is fixedly installed on the top of the sliding column 417. The bottom of the heating box 419 is slidably connected to the top of the mounting frame 411. A screw 418 is installed on the inner wall of the sliding column 417. One end of the screw 418 is movably penetrated through the inner wall of the reagent box body 1 and extends to the outside of the reagent box body 1, and a rotating disk 414 is installed for transmission.

[0041] In this embodiment, the bottom of the rectangular block 415 is slidably connected to the inner wall of the reagent box body 1, the side wall of the screw rod 1 412 is threadedly connected to the inner wall of the rectangular block 415, and the inner wall of the sliding column 1 417 is threadedly connected to the side wall of the screw rod 2 418.

[0042] In the specific implementation process, the operator can rotate the rotating disk 2 414 and the rotating disk 1 413 respectively as needed. When the rotating disk 2 414 is rotated, the rotating disk 2 414 will drive the position of the screw 2 418 to rotate. Since the side wall of the screw 2 418 is threadedly connected with the inner wall of the sliding column 1 417, the screw 2 418 can effectively drive the position of the sliding column 1 417 and the heating box 1 419 to slide on the inner wall of the installation frame 411 when rotating, so as to move them to the right side of the installation frame 411. The operator rotates the rotating disk 1 414. 13 is rotated, the rotating disk 1 413 drives the screw 1 412 to rotate, because the side wall of the screw 1 412 is threadedly connected with the inner wall of the rectangular block 415, the screw 1 412 can drive the rectangular block 415 to move to the right position when it rotates, and the rectangular block 415 drives the sliding column 2 4111 and the heating box 2 4110 to move under the action of the adjusting rod 416, and the heating box 2 4110 and the sliding column 2 4111 slide from left to right on the mounting frame 411, so that the operator can use the heating box 2 4110 conveniently.

[0043] Example 2: Based on Example 1, a preferred embodiment of the HDA isothermal amplification kit for detecting pine wood nematodes provided by the present invention is as follows: Figures 1 to 5As shown: the limiting mechanism 42 includes a mounting plate 423, the mounting plate 423 is fixedly mounted on the right side wall of the reagent box body 1, a plug-in rod 422 is slidably mounted on the inner wall of the mounting plate 423, a pull plate 424 is fixedly mounted on the bottom of the plug-in rod 422, a limiting plate 421 is slidably mounted on the top of the plug-in rod 422, the side wall of the limiting plate 421 is fixedly connected to the side wall of the protective cover 3, a connecting spring 425 is fixedly mounted on the bottom of the mounting plate 423, and the bottom of the connecting spring 425 is fixedly connected to the top of the pull plate 424.

[0044] In this embodiment, the diameter of the side wall of the plug rod 422 is the same as the inner wall diameter of the running groove provided on the inner wall of the limiting plate 421 , and the side wall of the plug rod 422 is slidably connected to the inner wall of the circular groove.

[0045] During the specific implementation process, when the operator uses the device, when the heating box needs to be used, and when the protective cover 3 needs to be opened, the pull plate 424 can be directly pulled down, and the pull plate 424 drives the plug-in rod 422 to slide on the inner wall of the limit plate 421. The pull plate 424 will also pull one end of the connecting spring 425. When one end of the plug-in rod 422 is separated from the inner wall of the limit plate 421, the operator can pull the position of the handle 5, and the handle 5 will drive the protective cover 3 to rotate on the top of the reagent box body 1. The position of the protective cover 3 can be effectively limited by the limiting mechanism 42.

[0046] Example 3: Based on Example 1 and Example 2, a preferred embodiment of the HDA isothermal amplification kit for detecting pine wood nematodes provided by the present invention is as follows: Figures 1 to 5 As shown: the auxiliary mechanism 43 includes a compression spring 431 and a limiting rod 433, one end of the compression spring 431 is fixedly connected to the inner wall of the mounting frame 411, the other end of the compression spring 431 is fixedly installed with a displacement plate 432, the side wall of the displacement plate 432 is slidably connected to the inner wall of the mounting frame 411, the limiting rod 433 is fixedly connected to the inner wall of the heating box 419, and the side wall of the limiting rod 433 is slidably installed with a partition 434.

[0047] In the specific implementation process, the displacement plate 432 and the compression spring 431 can enable the sliding column 4111 to effectively slide to the original position when it is not squeezed by the adjustment rod 416. By designing a partition 434 on the side wall of the limiting rod 433, different reagents can be effectively separated and stored.

[0048] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0049] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A HDA isothermal amplification kit for detecting pine wood nematodes, comprising a kit body (1), A pad (2) fixedly mounted on the bottom of the reagent kit body (1); and a storage component (4) arranged at the side of the reagent box body (1); characterized in that: The storage component (4) comprises an adjustment mechanism (41) arranged on the inner wall of the reagent box body (1); A limiting mechanism (42) is installed at the right side wall of the reagent box body (1); An auxiliary mechanism (43) is provided on the inner wall of the reagent box body (1), and the auxiliary mechanism (43) is connected to one end of the regulating mechanism (41).

2. The HDA isothermal amplification kit for detecting pine wood nematodes according to claim 1, characterized in that: A protective cover (3) is hingedly mounted on the top side wall of the reagent box body (1) via a hinge, the bottom of the protective cover (3) is arranged in contact with the top of the reagent box body (1), and a handle (5) is fixedly mounted on the top of the protective cover (3).

3. The HDA isothermal amplification kit for detecting pine wood nematodes according to claim 1, characterized in that: The adjustment mechanism (41) comprises a mounting frame (411), wherein the mounting frame (411) is fixedly mounted on the inner wall of the reagent box body (1), a sliding column 2 (4111) is slidably mounted on the inner wall of the mounting frame (411), a heating box 2 (4110) is fixedly mounted on the top of the sliding column 2 (4111), the bottom of the heating box 2 (4110) is slidably connected to the top of the mounting frame (411), an adjustment rod (416) is provided in contact with the side wall of the sliding column 2 (4111), a rectangular block (415) is fixedly mounted on one end of the adjustment rod (416), a screw 1 (412) is mounted on the inner wall of the rectangular block (415), and the screw 1 (412) is fixedly mounted on the inner wall of the rectangular block (415). One end of a screw rod (412) movably penetrates the inner wall of the reagent box body (1) and extends to the outside of the reagent box body (1), and is transmission-mounted with a rotating disk (413); a sliding column (417) is slidably mounted on the inner wall of the mounting frame (411); a heating box (419) is fixedly mounted on the top of the sliding column (417); the bottom of the heating box (419) is slidably connected to the top of the mounting frame (411); a screw rod (418) is mounted on the inner wall of the sliding column (417); one end of the screw rod (418) movably penetrates the inner wall of the reagent box body (1) and extends to the outside of the reagent box body (1), and is transmission-mounted with a rotating disk (414).

4. The HDA isothermal amplification kit for detecting pine wood nematodes according to claim 3, characterized in that: The bottom of the rectangular block (415) is slidably connected to the inner wall of the reagent box body (1), the side wall of the screw rod 1 (412) is threadedly connected to the inner wall of the rectangular block (415), and the inner wall of the sliding column 1 (417) is threadedly connected to the side wall of the screw rod 2 (418).

5. The HDA isothermal amplification kit for detecting pine wood nematodes according to claim 1, characterized in that: The limiting mechanism (42) comprises a mounting plate (423), wherein the mounting plate (423) is fixedly mounted on the right side wall of the reagent box body (1), a plug-in rod (422) is slidably mounted on the inner wall of the mounting plate (423), a pull plate (424) is fixedly mounted on the bottom of the plug-in rod (422), a limiting plate (421) is slidably mounted on the top of the plug-in rod (422), a side wall of the limiting plate (421) is fixedly connected to the side wall of the protective cover (3), a connecting spring (425) is fixedly mounted on the bottom of the mounting plate (423), and the bottom of the connecting spring (425) is fixedly connected to the top of the pull plate (424).

6. The HDA isothermal amplification kit for detecting pine wood nematodes according to claim 5, characterized in that: The diameter of the side wall of the plug-in rod (422) is the same as the diameter of the inner wall of the running groove provided on the inner wall of the limiting plate (421), and the side wall of the plug-in rod (422) is slidably connected to the inner wall of the circular groove.

7. The HDA isothermal amplification kit for detecting pine wood nematodes according to claim 1, characterized in that: The auxiliary mechanism (43) comprises a compression spring (431) and a limiting rod (433), one end of the compression spring (431) is fixedly connected to the inner wall of the mounting frame (411), the other end of the compression spring (431) is fixedly mounted with a displacement plate (432), the side wall of the displacement plate (432) is slidably connected to the inner wall of the mounting frame (411), the limiting rod (433) is fixedly connected to the inner wall of the heating box (419), and a partition (434) is slidably mounted on the side wall of the limiting rod (433).

8. The HDA isothermal amplification kit for detecting pine wood nematodes according to claim 1, characterized in that: There are four pads (2), the four pads (2) are of equal shape and size, and the four pads (2) are respectively fixedly mounted at the four corners of the bottom of the reagent box body (1).

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