Process and equipment for synthesizing semeglutide

Abstract

The equipment comprises a synthesis box, a culture medium is arranged in the middle of the inner side of the synthesis box, a stirring mechanism is arranged above the culture medium, the stirring mechanism is located on the inner wall of the synthesis box, the output end of the stirring mechanism is located on the inner side of the culture medium, a nutrition collection box is arranged on one side of the stirring mechanism, and the nutrition collection box is arranged on the other side of the synthesis box. One side of the bottom surface of the nutrition collection box is communicated with a measurement assembly, and the bottom of the measurement assembly is connected with an extraction conveying part; according to the device, various different nutrient solutions can be independently absorbed, a fixed amount of single nutrient solution is conveyed into the culture medium through the measuring assembly and the extracting and conveying part, absorption and growth of a carrier are provided, and after the nutrient solutions are supplemented, stirring and mixing of the nutrient solutions in the culture medium can be automatically completed by replacing different nutrient solutions, so that the nutrient solutions are completely supplemented. And the growth of the carrier is completely mechanically and automatically operated, so that the manual intervention is reduced, and the operation precision and the culture efficiency are higher.

Description

Technical Field

[0001] The present invention relates to the field of semaglutide synthesis, and in particular to a semaglutide synthesis process and equipment. Background Art

[0002] Semaglutide is a drug used to treat type 2 diabetes and is also used for weight management. It is a glucagon-like peptide-1 (GLP-1) receptor agonist that stimulates insulin secretion and inhibits glucagon secretion by mimicking the effects of GLP-1, thereby lowering blood sugar levels. In addition, semaglutide can slow gastric emptying, increase satiety, and help with weight control.

[0003] The synthesis process of semaglutide mainly includes biosynthesis and chemical synthesis. The biosynthesis method is to complete the synthesis of semaglutide through the steps of construction of genetically engineered strains, fermentation, protein purification, chemical synthesis, product refining and analysis.

[0004] The construction of genetically engineered strains during the synthesis of semaglutide is to clone the gene sequence of semaglutide into a suitable expression vector (Escherichia coli) and achieve the production of the target protein through a fermentation process. However, during the fermentation of the vector, the vector requires a good culture environment and the supplementation of culture components. The existing fermentation method requires manual control of the nutritional supplement required for the culture, which makes the synthesis steps of semaglutide more complicated, the operation requirements are high, and it is difficult to synthesize quickly.

[0005] Therefore, we made improvements to this and proposed a synthesis process and equipment for semaglutide. Summary of the invention

[0006] The purpose of the present invention is to address the high requirements for the supplementation operation of the existing carrier culture nutrients.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following synthesis process of semaglutide and its equipment to improve the above-mentioned problems.

[0008] The specific application is as follows:

[0009] A synthesis process and equipment of semaglutide, comprising a synthesis box, wherein a culture medium is provided in the middle of the inner side of the synthesis box, a stirring mechanism is provided above the culture medium, the stirring mechanism is located on the inner wall of the synthesis box, the output end of the stirring mechanism is located on the inner side of the culture medium, a nutrient collection box is provided on one side of the stirring mechanism, a measuring component is connected to one side of the bottom surface of the nutrient collection box, an extraction and conveying part is connected to the bottom of the measuring component, suction is provided to the inside of the measuring component when the extraction and conveying part is started, an energy storage box is provided on the upper side of one end of the extraction and conveying part away from the measuring component, the side of the energy storage box away from the extraction and conveying part is connected to the inside of the nutrient collection box, and high-pressure air is provided to the inside of the nutrient collection box when the energy storage box is started;

[0010] As a preferred technical solution of the present application, a liquid storage tank is provided on the inner side of the front face of the synthesis box, nutrient bottles arranged in sequence are placed inside the liquid storage tank, a control panel is provided directly above the liquid storage tank, the control panel is fixed on the top surface of the synthesis box, a synthesis chamber is provided on the inner side of the liquid storage tank, the culture medium is located at the inner bottom of the synthesis chamber, the stirring mechanism is located at the top surface of the synthesis chamber, a collecting bucket is provided on one side of the top of the culture medium, the collecting bucket is located directly below the measuring component, the collecting bucket is connected with the inner side of the culture medium, a purification box is provided on the side of the culture medium away from the collecting bucket, and the purification box is connected with the bottom of the outer wall of the collecting bucket.

[0011] As a preferred technical solution of the present application, the stirring mechanism includes a driving motor, which is fixed on the inner side of the top surface of the synthesis chamber, the output end of the driving motor is connected to a driving gear, the bottom surface of the driving gear is connected to a driving shaft, the bottom end of the driving shaft is connected to a stirring plate, the bottom surface of the stirring plate is connected to annular stirring rods distributed equidistantly, the stirring rods are penetrated into the inner side of the culture medium, a speed gear is provided on the side of the driving gear close to the nutrient collection box, the speed gear is rotatably connected to the top surface of the synthesis chamber, the speed gear is meshed with the driving gear, the bottom surface of the speed gear is connected to a transmission pulley, a transmission belt is sleeved on the outer side of the transmission pulley, and the other end of the transmission belt is transmission-sleeved with the nutrient collection box.

[0012] As a preferred technical solution of the present application, the nutrient collection box includes a collection tray, a ring-shaped fixed rod is fixed on the outer side of the top surface of the collection tray, the other end of the fixed rod is fixed to the top surface of the synthesis chamber, a collection groove is provided on the inner side of the collection tray, an adjustment tray is fitted and slidably sealed on the top of the inner side of the collection groove, an adjustment shaft is fixed at the center of the top surface of the adjustment tray, the other end of the adjustment shaft is rotatably connected to the inner side of the top surface of the synthesis chamber, an annular equidistantly distributed nutrient tube is connected to the outer side of the top surface of the collection tray, one end of the nutrient tube connected to the collection tray is communicated with the inside of the collection groove, and the other end of the nutrient tube is respectively passed through the inner sides of multiple nutrient bottles inside the liquid storage tank, a first one-way valve is provided on the lower side of a single nutrient tube inside the adjustment tray, the top of the first one-way valve is communicated with the inside of the nutrient tube, and the bottom of the first one-way valve is communicated with the lower part of the inner side of the collection groove, a driven pulley is fixed on the top of the outer wall of the adjustment shaft, and one end of the transmission belt away from the transmission pulley is sleeved on the outer side of the driven pulley, a liquid outlet is provided on one side of the bottom surface of the collection tray, and the bottom of the liquid outlet is communicated with the inner side of the measuring component.

[0013] As a preferred technical solution of the present application, the first one-way valve includes a valve stem, both ends of which are fixed to the inner wall of the adjusting disk, symmetrical valve plates are provided on both sides of the middle part of the valve stem, a fixedly connected hinge rod is passed through the inner side of one end of the valve plate close to the valve stem, a return spring is sleeved on the outer side of the hinge rod, the position of the hinge rod sleeved with the return spring is hinged to the inner side of the valve stem, and both ends of the return spring are respectively fixed to the outer wall of the hinge rod and the inner wall of the valve stem.

[0014] As a preferred technical solution of the present application, the measuring component includes a measuring tube, the top of the measuring tube is fixed to the bottom surface of the nutrient collection box, a ring-shaped measuring cylinder is opened in the middle of the inner side of the measuring tube, and liquid collecting grooves are opened at the upper and lower ends of the measuring cylinder. The top of the liquid collecting groove is connected with the liquid outlet, and the bottom of the liquid collecting groove is connected with the measuring cylinder. A liquid spray nozzle is opened on one side of the bottom of the measuring tube, and the liquid spray nozzle is connected with the inside of the liquid collecting groove. A drip cone block is provided below the liquid spray nozzle, and the drip cone block is fixed to the bottom surface of the measuring tube.

[0015] As a preferred technical solution of the present application, the extraction and conveying part includes an extraction tube, a sealing plug is slidably fitted on the inner side of the extraction tube, a pulling screw is connected to the center of one side of the sealing plug for limited rotation, a driving motor is fixed to the outer wall of the end of the extraction tube away from the sealing plug, a driving screw is connected to the output end of the driving motor, the driving screw is meshed with the driving motor, and an end of the extraction tube connected to the sealing plug is connected to a pneumatic tube, and the end of the pneumatic tube away from the extraction tube is connected to the inner side of the liquid collecting tank.

[0016] As a preferred technical solution of the present application, the side of the driving screw away from the driving motor is connected to an energy storage driving rod, the outer surface of the energy storage driving rod is sleeved with an energy storage pulley, the outer side of the energy storage pulley is sleeved with an energy storage transmission belt, and the other end of the energy storage transmission belt is transmission-connected to the energy storage box.

[0017] The cam is an airtight container, the air outlet is located at the top of the container, and the container is connected to the air outlet of the container, and the container is closed by the air filter ...

[0018] As a preferred technical solution of the present application, an energy storage transmission part is provided below the energy storage box, and the energy storage transmission part includes an energy storage transmission shaft, and both ends of the energy storage transmission shaft are rotatably connected to the inner wall of the synthesis chamber, and energy storage wheels are fixed to the outer walls of both sides of the energy storage transmission shaft located below the energy storage box, and a belt pulley is provided between the energy storage wheels, and the belt pulley is fixedly sleeved on the outer wall of the energy storage transmission shaft, and one end of the energy storage transmission belt away from the energy storage belt pulley is sleeved on the outside of the belt pulley, and an energy storage toggle rod is fixed on the upper side of the outer wall of the energy storage wheel, and the energy storage toggle rod is inserted between the positioning rod and the one-way elastic block.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] In the scheme of the present application: through the setting of the nutrient collection box inside the synthesis chamber, a variety of different nutrient solutions can be absorbed separately, and a fixed amount of a single nutrient solution can be delivered to the inside of the culture medium through the measuring component and the extraction and delivery part to provide the absorption and growth of the carrier, and after the nutrient solution is replenished, the stirring and mixing of the nutrient solution inside the culture medium can be automatically completed by replacing different nutrient solutions, so that the growth of the carrier is completely mechanically and fully automated, reducing human intervention, and the operation accuracy and culture efficiency are higher.

[0021] 1. The present invention is provided with a stirring mechanism. After the nutrient solution enters the culture medium, the driving motor can be used to drive the stirring plate to rotate, so that the stirring rod stirs and mixes the nutrient solution and the carrier inside the culture medium, so that the nutrient solution is evenly transported to multiple positions inside the culture medium, avoiding the accumulation of carriers, and improving the culture safety of the carriers and the growth efficiency of the carriers.

[0022] 2. The present invention provides a nutrient collection box at the inner top of the synthesis chamber, which can collect multiple nutrient solutions and transport the nutrient solution of a single nutrient tube to the inside of the nutrient collection box, thereby realizing automatic transportation of nutrient solution to the culture medium, reducing the manual operation process, reducing the difficulty of carrier culture operation, and enabling large-scale rapid culture.

[0023] 3. The present invention has a measuring component connected to the bottom surface of the nutrient collection box, which can absorb the nutrient solution inside the nutrient collection box through the measuring tube and measuring cylinder inside the measuring component, and use the measuring cylinder to achieve high-precision extraction of the nutrient solution and transport it to the inside of the culture medium, thereby improving the culture quality of the carrier and increasing the amount of liquid extracted to lower the pH value, thereby ensuring that the culture environment inside the culture medium is in a safe culture state. At the same time, the opening of the liquid collecting tank allows the nutrient solution to adhere to the inner wall of the liquid collecting tank through its own viscosity, thereby avoiding liquid flow down and ensuring the accuracy of nutrient solution extraction.

[0024] 4. The present invention provides an extraction and conveying part at the bottom of the measuring component, which can provide suction for the interior of the measuring component to absorb the nutrient solution, allowing the nutrient solution to enter the inner side of the measuring component. At the same time, the extraction and conveying part utilizes the meshing drive of the pulling screw and the driving screw to control the sliding distance of the sealing plug with high precision, and accurately control the suction of the sealing plug on the interior of the measuring component, thereby ensuring that the measuring component has a high-precision nutrient solution absorption function.

[0025] 5. The present invention provides an energy storage box between the extraction and conveying part and the nutrient collection box. After the extraction and conveying part drives the measuring component to absorb the nutrient solution, it can simultaneously drive the energy storage block inside the energy storage box to slide when it rotates in reverse to reset the sealing plug, so that air and rebound tension are accumulated on one side of the energy storage box. When the driving screw is reversely rotated 90°, the energy storage box can complete the energy storage and release, and the accumulated air is filled into the inner side of the nutrient collection box in a high-pressure state, and all the nutrient solution absorbed in the measuring cylinder is sent into the inner side of the culture medium, completing the automatic addition of the nutrient solution.

[0026] 6. In the present invention, through the transmission connection between the stirring mechanism and the nutrient collection box, after the energy storage box transports the nutrient solution inside the measuring component, the stirring mechanism can drive the collection plate inside the nutrient collection box to rotate while stirring the nutrient solution, thereby changing the connectivity between the first one-way valve and other nutrient solution connecting ports, thereby realizing the function of replacing, extracting and transporting multiple nutrient solutions. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 A schematic structural diagram of a preferred embodiment of a synthesis process and equipment of semaglutide provided by the present invention;

[0028] Figure 2 for Figure 1 A schematic cross-sectional view of the structure of the synthesis chamber shown;

[0029] Figure 3 for Figure 2 The schematic diagram of the structure inside the synthesis chamber shown;

[0030] Figure 4 for Figure 3 The enlarged schematic diagram of the side view structure of the nutrition bottle shown;

[0031] Figure 5 for Figure 4 The structure of the nutrient collection box and the enlarged schematic diagram of the structure of the extraction and transportation part are shown;

[0032] Figure 6 for Figure 5 The structure of the nutrient collection box shown is an exploded and enlarged schematic diagram of the cross-section of the middle part;

[0033] Figure 7 for Figure 6 The structure of the first one-way valve is shown in an enlarged cross-sectional schematic diagram;

[0034] Figure 8 for Figure 5 The structural cross-sectional enlarged schematic diagram of the measuring component shown;

[0035] Fig. 9 for Figure 5 An enlarged schematic diagram of the structure of the energy storage box shown;

[0036] Fig.10 for Fig. 9 The schematic diagram of the structure of the energy storage box is shown as an exploded and enlarged cross-sectional view;

[0037] Fig.11 for Figure 5 The structural schematic diagram of the stirring mechanism shown;

[0038] Fig.12 for Figure 8 The structure of the measuring component and the extraction and conveying part is shown in an enlarged schematic cross-sectional view.

[0039] Indicated in the figure:

[0040] 1. Synthesis box; 11. Liquid storage tank; 12. Synthesis room; 13. Control panel; 14. Nutrition bottle;

[0041] 2. Culture medium; 21. Collection bucket; 22. Purification box;

[0042] 3. stirring mechanism; 31. driving motor; 32. driving gear; 33. driving shaft; 34. stirring plate; 35. stirring rod; 36. speed change gear; 37. driving pulley; 38. driving belt;

[0043] 4. Nutrition collection box; 41. Collection plate; 42. Fixed rod; 43. Collection slot; 44. Nutrition tube; 45. Adjustment shaft; 46. Adjustment plate; 47. First one-way valve; 471. Valve stem; 472. Valve plate; 473. Hinge rod; 474. Return spring; 48. Driven pulley; 49. Liquid outlet;

[0044] 5. Measuring assembly; 51. Measuring tube; 52. Liquid collecting tank; 53. Measuring cylinder; 54. Liquid spraying nozzle; 55. Dropping cone block;

[0045] 6. Extraction and conveying part; 61. Extraction pipe; 62. Sealing plug; 63. Drawing screw; 64. Driving motor; 65. Driving screw; 66. Energy storage driving rod; 67. Energy storage pulley; 68. Energy storage transmission belt; 69. Air pressure pipe;

[0046] 7. Energy storage box; 71. Energy storage block; 72. Air vent; 73. Second one-way valve; 74. Exhaust pipe; 75. Third one-way valve; 76. Positioning rod; 77. Energy storage transmission part; 771. Energy storage transmission shaft; 772. Energy storage wheel; 773. Belt pulley; 774. Energy storage toggle rod; 78. One-way elastic stop block; 79. Tension spring. DETAILED DESCRIPTION

[0047] In order to enable those skilled in the art to better understand the scheme of the present invention, the technical scheme in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0048] As described in the background art, the operation requirements for supplementing nutrients in carrier culture are high.

[0049] In order to solve this technical problem, the present invention provides a synthesis process of semaglutide and its equipment, which are used to automatically supplement the nutrient solution inside the culture medium, and quickly and accurately supplement the culture medium with multiple nutrient solutions for carrier growth and stir and mix the culture medium.

[0050] Specifically, please refer to Figure 1-Figure 12 The synthesis process and equipment of semaglutide specifically include:

[0051] A synthesis box 1, wherein a culture medium 2 is provided in the middle of the inner side of the synthesis box 1, a stirring mechanism 3 is provided above the culture medium 2, the stirring mechanism 3 is located on the inner wall of the synthesis box 1, the output end of the stirring mechanism 3 is located on the inner side of the culture medium 2, a nutrient collection box 4 is provided on one side of the stirring mechanism 3, a measuring component 5 is connected to one side of the bottom surface of the nutrient collection box 4, an extraction and conveying part 6 is connected to the bottom of the measuring component 5, and suction is provided to the inside of the measuring component 5 when the extraction and conveying part 6 is started, an energy storage box 7 is provided on the upper side of one end of the extraction and conveying part 6 away from the measuring component 5, and a side of the energy storage box 7 away from the extraction and conveying part 6 is connected to the inner side of the nutrient collection box 4, and high-pressure air is provided to the inside of the nutrient collection box 4 when the energy storage box 7 is started;

[0052] The present invention provides a synthesis process and equipment for semaglutide. Through the arrangement of a nutrient collection box 4 inside a synthesis chamber 12, a plurality of different nutrient solutions can be absorbed separately, and a fixed amount of a single nutrient solution can be delivered to the inside of a culture medium 2 through a measuring component 5 and an extraction and delivery part 6 to provide absorption and growth of the carrier. After the nutrient solution is replenished, the stirring and mixing of the nutrient solution inside the culture medium 2 can be automatically completed by replacing different nutrient solutions, so that the growth of the carrier is completely mechanically and fully automated, thereby reducing human intervention and improving operation accuracy and culture efficiency.

[0053] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings.

[0054] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features and technical solutions in the embodiments may be combined with each other.

[0055] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0056] Example 1

[0057] Please refer to Figure 1-Figure 12A synthesis process and equipment of semaglutide, wherein a liquid storage tank 11 is provided on the inner side of the front of the synthesis box 1, and nutrient bottles 14 are placed in sequence on the inner side of the liquid storage tank 11, and a control panel 13 is provided directly above the liquid storage tank 11, and the control panel 13 is fixed on the top surface of the synthesis box 1, and a synthesis chamber 12 is provided on the inner side of the liquid storage tank 11, and the culture medium 2 is located at the inner bottom of the synthesis chamber 12, and the stirring mechanism 3 is located at the inner top surface of the synthesis chamber 12, and a collecting bucket 21 is provided on one side of the top of the culture medium 2, and the collecting bucket 21 is located directly below the measuring component 5, and the collecting bucket 21 is connected with the inner side of the culture medium 2, and a purification box 22 is provided on the side of the culture medium 2 away from the collecting bucket 21, and the purification box 22 is connected with the bottom of the outer wall of the collecting bucket 21.

[0058] The stirring mechanism 3 includes a driving motor 31, which is fixed on the inner side of the top surface of the synthesis chamber 12. The output end of the driving motor 31 is connected to a driving gear 32. The bottom surface of the driving gear 32 is connected to a driving shaft 33. The bottom end of the driving shaft 33 is connected to a stirring disk 34. The bottom surface of the stirring disk 34 is connected to annular stirring rods 35 that are evenly distributed. The stirring rods 35 are inserted into the inner side of the culture medium 2. A speed change gear 36 is provided on the side of the driving gear 32 close to the nutrient collection box 4. The speed change gear 36 is rotatably connected to the top surface of the synthesis chamber 12. The speed change gear 36 is meshed with the driving gear 32. A transmission pulley 37 is connected to the bottom surface of the speed change gear 36. A transmission belt 38 is sleeved on the outer side of the transmission pulley 37. The other end of the transmission belt 38 is transmission sleeved with the nutrient collection box 4.

[0059] By setting up the stirring mechanism 3, after the nutrient solution enters the culture medium 2, the driving motor 31 can be used to drive the stirring disk 34 to rotate, so that the stirring rod 35 can stir and mix the nutrient solution and the carrier inside the culture medium 2, so that the nutrient solution is evenly transported to multiple positions inside the culture medium 2, thereby avoiding the accumulation of carriers and improving the culture safety and growth efficiency of the carriers.

[0060] Example 2

[0061] The synthesis process and equipment of semaglutide provided in Example 1 were further optimized. Specifically, Figure 1-Figure 12The nutrient collection box 4 includes a collection plate 41, a ring-shaped fixed rod 42 is fixed to the outer side of the top surface of the collection plate 41, and the other end of the fixed rod 42 is fixed to the top surface of the synthesis chamber 12. A collection groove 43 is provided on the inner side of the collection plate 41, and an adjustment plate 46 is sealed and slidably arranged on the top of the inner side of the collection groove 43. An adjustment shaft 45 is fixed at the center of the top surface of the adjustment plate 46, and the other end of the adjustment shaft 45 is rotatably connected to the inner side of the top surface of the synthesis chamber 12. The outer side of the top surface of the collection plate 41 is connected to an annular equidistantly distributed nutrient tube 44, and the nutrient tube 44 connects one end of the collection plate 41 with the inside of the collection groove 43 The other ends of the nutrition tubes 44 are respectively passed through the inner sides of the multiple nutrition bottles 14 inside the liquid storage tank 11, and the first one-way valve 47 is provided inside the adjusting disk 46 at the lower side of the single nutrition tube 44. The top of the first one-way valve 47 is connected with the inside of the nutrition tube 44, and the bottom of the first one-way valve 47 is connected with the lower inner side of the collecting tank 43. A driven pulley 48 is fixed to the top of the outer wall of the adjusting shaft 45, and one end of the transmission belt 38 away from the transmission pulley 37 is fitted on the outer side of the driven pulley 48. A liquid outlet 49 is provided on one side of the bottom surface of the collecting disk 41, and the bottom of the liquid outlet 49 is connected with the inner side of the measuring component 5.

[0062] The first one-way valve 47 includes a valve stem 471, both ends of which are fixed to the inner wall of the adjusting disk 46, and symmetrical valve plates 472 are provided on both sides of the middle of the valve stem 471. A fixedly connected hinge rod 473 is passed through the inner side of one end of the valve plate 472 close to the valve stem 471, and a return spring 474 is sleeved on the outer side of the hinge rod 473. The position where the hinge rod 473 sleeves the return spring 474 is hinged to the inner side of the valve stem 471, and both ends of the return spring 474 are respectively fixed to the outer wall of the hinge rod 473 and the inner wall of the valve stem 471.

[0063] A nutrient collection box 4 is provided at the inner top of the synthesis chamber 12, which can collect multiple nutrient solutions and transport the nutrient solution from a single nutrient tube to the inside of the nutrient collection box, thereby realizing automatic transport of nutrient solution to the culture medium 2, reducing the manual operation process, reducing the difficulty of carrier culture operation, and enabling large-scale rapid culture.

[0064] Example 3

[0065] The synthesis process and equipment of semaglutide provided in Example 1 or 2 are further optimized. Specifically, Figure 1-Figure 12As shown, the measuring component 5 includes a measuring tube 51, the top of which is fixed to the bottom surface of the nutrient collection box 4, a measuring cylinder 53 distributed in an annular manner is provided in the middle of the inner side of the measuring tube 51, and liquid collecting grooves 52 are provided at the upper and lower ends of the measuring cylinder 53. The top of the liquid collecting groove 52 is connected to the liquid outlet 49, and the bottom of the liquid collecting groove 52 is connected to the measuring cylinder 53. A liquid spray nozzle 54 is provided on one side of the bottom of the measuring tube 51, and the liquid spray nozzle 54 is connected to the inside of the liquid collecting groove 52. A drip cone block 55 is provided below the liquid spray nozzle 54, and the drip cone block 55 is fixed to the bottom surface of the measuring tube 51.

[0066] The bottom surface of the nutrient collection box 4 is connected to a measuring component 5, which can absorb the nutrient solution inside the nutrient collection box through the measuring tube 51 and the measuring cylinder 53 inside the measuring component 5, and use the measuring cylinder 53 to achieve high-precision extraction of the nutrient solution and transport it to the inside of the culture medium 2, thereby improving the culture quality of the carrier. At the same time, it can increase the extraction amount of the liquid medicine that lowers the pH value, thereby ensuring that the culture environment inside the culture medium 2 is in a safe culture state. At the same time, the opening of the liquid collecting tank 52 can allow the nutrient solution to adhere to the inner wall of the liquid collecting tank 52 through its own viscosity, thereby avoiding the liquid from flowing down and ensuring the accuracy of nutrient solution extraction.

[0067] Example 4

[0068] The synthesis process and equipment of semaglutide provided in Examples 1-3 were further optimized. Specifically, Figure 1-Figure 12 As shown, the extraction and conveying part 6 includes an extraction tube 61, a sealing plug 62 is slidably fitted on the inner side of the extraction tube 61, and a pulling screw 63 is connected to the center of one side of the sealing plug 62 for limited rotation. A driving motor 64 is fixed to the outer wall of the end of the extraction tube 61 away from the sealing plug 62, and a driving screw 65 is connected to the output end of the driving motor 64, and the driving screw 65 is meshed with the driving motor 64. The end of the extraction tube 61 connected to the sealing plug 62 is connected to a pneumatic tube 69, and the end of the pneumatic tube 69 away from the extraction tube 61 is connected to the inner side of the liquid collecting tank 52.

[0069] The side of the driving screw 65 away from the driving motor 64 is connected to an energy storage driving rod 66, and the outer surface of the energy storage driving rod 66 is sleeved with an energy storage pulley 67. The outer side of the energy storage pulley 67 is sleeved with an energy storage transmission belt 68, and the other end of the energy storage transmission belt 68 is transmission-connected to the energy storage box 7.

[0070] An extraction and conveying part 6 is provided at the bottom of the measuring component 5, which can provide suction for the inside of the measuring component 5 to absorb the nutrient solution, allowing the nutrient solution to enter the inside of the measuring component 5. At the same time, the extraction and conveying part 6 is driven by the engagement of the pulling screw 63 and the driving screw 65, so as to control the sliding distance of the sealing plug 62 with high precision, and accurately control the suction of the sealing plug 62 on the inside of the measuring component 5, thereby ensuring that the measuring component 5 has a high-precision nutrient solution absorption function.

[0071] Example 5

[0072] The synthesis process and equipment of semaglutide provided in Examples 1-4 were further optimized. Specifically, Figure 1-Figure 12 As shown, an energy storage block 71 is slidably connected to the inner side of the energy storage box 7, and a vent 72 with two ends passing through is opened at the center of the energy storage block 71. A second one-way valve 73 is provided on the inner side of the top of one end of the vent 72. An air outlet pipe 74 is connected to the top of the end of the outer wall of the energy storage box 7 connected to the second one-way valve 73. A third one-way valve 75 is fixed to the position where the energy storage box 7 is connected to the air outlet pipe 74. The end of the air outlet pipe 74 away from the energy storage box 7 is communicated with the inner side of the liquid outlet 49. Symmetrical one-way elastic stoppers are fixed to the outer walls of both sides of the energy storage block 71 78, one end of the one-way elastic stopper 78 elastically slides on the inner side of the energy storage block 71, and the other end of the one-way elastic stopper 78 penetrates the outer wall of the energy storage box 7 and extends to the outside of the energy storage box 7. The end of the one-way elastic stopper 78 located on the outside of the energy storage box 7 slides on the inner side of the energy storage box 7, and a positioning rod 76 is fixed to the outer wall of the energy storage box 7 near the one-way elastic stopper 78. Symmetrical tension springs 79 are fixed on both sides of the bottom of one end of the energy storage box 7 near the second one-way valve 73, and the other end of the tension spring 79 is fixed to the outer wall of the energy storage block 71.

[0073] An energy storage transmission part 77 is provided below the energy storage box 7, and the energy storage transmission part 77 includes an energy storage transmission shaft 771. Both ends of the energy storage transmission shaft 771 are rotatably connected to the inner wall of the synthesis chamber 12. The energy storage transmission shaft 771 is located below the energy storage box 7 and has energy storage wheels 772 fixed on the outer walls on both sides. A belt pulley 773 is provided between the energy storage wheels 772. The belt pulley 773 is fixedly sleeved on the outer wall of the energy storage transmission shaft 771. One end of the energy storage transmission belt 68 away from the energy storage pulley 67 is sleeved on the outside of the belt pulley 773. An energy storage toggle rod 774 is fixed on the upper side of the outer wall of the energy storage wheel 772. The energy storage toggle rod 774 is penetrated between the positioning rod 76 and the one-way elastic block 78.

[0074] An energy storage box 7 is provided between the extraction and conveying part 6 and the nutrient collection box 4. After the extraction and conveying part 6 drives the measuring component 5 to complete the absorption of the nutrient solution, when the sealing plug 62 is reset by reverse rotation, the energy storage block 71 inside the energy storage box 7 can be driven to slide at the same time, so that air and rebound tension are accumulated on one side of the energy storage box 7. When the driving screw 65 is reversely rotated 90°, the energy storage box 7 can complete the energy storage and release, and the accumulated air is filled into the inner side of the nutrient collection box 4 in a high-pressure state, and all the nutrient solution absorbed in the measuring cylinder 53 is sent into the inner side of the culture medium 2, thereby completing the automatic addition of the nutrient solution.

[0075] Example 6

[0076] The present invention also discloses a synthesis process of semaglutide, comprising the following steps:

[0077] S1: Synthetic fusion of a polypeptide encoding gene containing the semaglutide precursor sequence;

[0078] This process first obtains the amino acid sequence of semaglutide and determines the sequence required for precursor synthesis;

[0079] Fusion has a well-expressed characteristic protein;

[0080] Designing a gene encoding a fusion polypeptide based on the amino acid sequence of the selected fusion partner and semaglutide precursor;

[0081] Optimize gene sequences through software tools;

[0082] After the design is completed, the gene encoding the fusion polypeptide is obtained by chemical synthesis;

[0083] S2: Connect the synthesized coding gene to the expression vector to construct a recombinant expression vector containing the coding gene. This vector is selected to be Escherichia coli:

[0084] S3: Inject the vector (E. coli) containing the coding gene into the culture medium;

[0085] S4: Place the components required for carrier culture in the culture medium, add inducers and components required for fermentation (carbon source, nitrogen source, inorganic salt);

[0086] Carbon sources included yeast powder, glucose, glycerol, and lactose;

[0087] Nitrogen sources include organic nitrogen sources (peptone) and inorganic nitrogen sources (ammonium salts, nitrates)

[0088] Inorganic salts are used to maintain pH and osmotic pressure;

[0089] S5: After fermentation, the target polypeptide is separated from the culture broth by protein purification technology;

[0090] S6: The purified peptide is modified through chemical synthesis steps, including protection and deprotection of side chains, formation of amide bonds, etc., to obtain semaglutide molecules with the correct three-dimensional structure:

[0091] S7: The synthesized semaglutide undergoes further purification steps to improve the purity and quality of the product, and analytical methods are used to ensure that the structure and activity of the product meet the requirements.

[0092] Working steps:

[0093] The culture medium 2 containing the carrier is placed inside the synthesis chamber 12, and then the control panel 13 is controlled according to the nutrition required by the carrier to start the drive motor 64 inside the extraction and conveying part 6. When the drive motor 64 is started, it drives the drive screw 65 and the energy storage drive rod 66 to rotate.

[0094] The rotation of the driving screw 65 will drive the pulling screw 63 meshing with it to rotate, and the rotation of the pulling screw 63 will drive the sealing plug 62 to slide on the inner wall of the extraction tube 61, and the sealing plug 62 will provide suction to the inside of the air pressure tube 69, and the air pressure tube 69 will absorb the air inside the measuring cylinder 53 inside the measuring component 5, so that the measuring cylinder 53 provides suction to the inside of the collecting tank 43. When the inside of the collecting tank 43 is in a high pressure state, the first one-way valve 47 inside the regulating disk 46 will open, so that the inside of the collecting tank 43 The nutrient solution inside the nutrient tube 44 connected to the first one-way valve 47 is absorbed. The nutrient solution will move to the inside of the measuring cylinder 53 under the continuous absorption of the sealing plug 62. When the nutrient solution to be absorbed arrives, the driving motor 64 is started to rotate in the reverse direction. Then the driving screw 65 will drive the pulling screw 63 to rotate in the reverse direction, pushing the sealing plug 62 to slide toward the inside of the extraction tube 61, and the extracted air will be discharged into the inside of the air pressure tube 69. At this time, the discharged air will be discharged through the liquid spray nozzle 54 connected to the liquid collecting tank 52.

[0095] It should be noted that: according to the amount of nutrient solution that needs to be absorbed, the moving distance of the sealing plug 62 can be controlled by starting the driving rotation stroke of the driving motor 64, so that the amount of air extracted by the sealing plug 62 can be accurately controlled, thereby achieving high-precision nutrient solution extraction.

[0096] When the driving motor 64 rotates in the reverse direction, the energy storage driving rod 66 will drive the energy storage pulley 67 to rotate, and the energy storage pulley 67 will drive the belt pulley 773 to rotate through the energy storage transmission belt 68. When the belt pulley 773 rotates, the energy storage wheel 772 coaxially connected to the belt pulley 773 will rotate synchronously. When the energy storage wheel 772 rotates, the energy storage toggle rod 774 connected to the top of the energy storage wheel 772 will rotate and push the one-way elastic block 78 to slide. When the one-way elastic block 78 slides, it will drive the energy storage block 71 to slide on the inner side of the energy storage box 7. During the sliding process of the energy storage block 71, tension will be applied to the tension spring 79, allowing the tension spring 79 to continue to have elastic force. At the same time, when the energy storage block 71 slides, due to the front of the energy storage box 7 When the energy storage block 71 is in a sealed state, the second one-way valve 73 at the end of the energy storage block 71 will open, and air will be provided to the front of the energy storage block 71 through the vent 72. When the energy storage toggle rod 774 is rotated to nearly 90°, the energy storage toggle rod 774 will be separated from the one-way elastic stopper 78. At this time, under the pulling of the tension spring 79, the air in front of the energy storage block 71 will be squeezed and filled into the interior of the liquid outlet 49 through the third one-way valve 75 in a high-pressure state. The bottom of the regulating disk 46 at the upper part of the liquid outlet 49 is provided with a first one-way valve 47 to seal the nutrient tube 44. Then, air will be filled into the interior of the liquid collecting tank 52 through the liquid outlet 49, and all the nutrient solution in the measuring cylinder 53 will be sprayed to the position of the liquid spray nozzle 54, thereby completing the addition of nutrient solution.

[0097] It should be noted that: when the drive motor 64 is in the driving state, when the energy storage toggle rod 774 inside the energy storage transmission part 77 rotates to the limit, the transmission engaged on the outside of the belt pulley 773 will slide with the outer wall of the belt pulley 773, so that the drive motor 64 can no longer drive it, and at the same time will not affect the driving work of the drive motor 64 to drive the pulling screw 63.

[0098] It should be noted that after the energy of the energy storage box 7 is released, the reverse rotation of the driving screw 65 drives the sealing plug 62 to reset. When the nutrient solution inside the measuring component 5 is sprayed out, air can be provided to the position where the extraction tube 61 is connected to the measuring component 5 to transport air to the inside of the measuring component 5, thereby preventing the nutrient solution from entering the inside of the extraction tube 61. When the sealing plug 62 is reset, since a pulley is used between the energy storage box 7 and the extraction and conveying part 6, the energy storage toggle rod 774 will stop rotating after being blocked by the positioning rod 76.

[0099] When the measuring component 5 is to be drawn with nutrient solution again, the energy storage toggle rod 774 rotates to the outer wall of the one-way elastic block 78, and the energy storage toggle rod 774 will push the one-way elastic block 78 to retract to the inside of the energy storage block 71. When the energy storage toggle rod 774 completely enters between the positioning rod 76 and the one-way elastic block 78, the one-way elastic block 78 will be ejected outward by the elastic force, thereby forming elastic energy storage again.

[0100] When a nutrient solution is added, the driving motor 31 is started to drive the driving gear 32 to rotate. When the driving gear 32 rotates, it will synchronously drive the driving shaft 33 and the speed change gear 36 to rotate.

[0101] When driven to rotate, the stirring disk 34 is driven to rotate, and the rotation of the stirring disk 34 is driven to rotate the stirring rod 35, and the rotation of the stirring rod 35 is used to stir and mix the culture components inside the culture medium 2 to which the nutrient solution is added.

[0102] While the stirring shaft rotates, the driving gear 32 drives the speed change gear 36 to rotate. The rotation of the speed change gear 36 will drive the coaxial transmission pulley 37 to rotate. The rotation of the transmission pulley 37 will drive the driven pulley 48 to rotate through the transmission belt 38. When the impulse pulley rotates, the coaxially connected adjustment shaft 45 will rotate. When the adjustment shaft 45 rotates, the adjustment disk 46 coaxial with it will rotate. The rotation of the adjustment disk 46 will move the opening with the first one-way valve 47 to the bottom of another nutrient tube 44 on the side of the collecting tank 43. Under the change of the stroke of the speed change gear 36, the driving gear 32 rotates multiple circles to stir the culture medium 2 evenly, and then the first one-way valve 47 will be moved to the bottom of the second nutrient tube 44. At this time, continue the above-mentioned extraction and conveying part 6 start-up process to allow the nutrient solution to continue to be added to the inside of the culture medium 2.

[0103] By continuing the above two steps, multiple nutrient solutions can be added. When the nutrient solution is added, the carrier inside the culture medium 2 is fermented and the fermented carrier can be absorbed into the inner side through the purification box 22 to perform chemical synthesis steps for synthesis and purification.

[0104] In the present invention, through the transmission connection between the stirring mechanism 3 and the nutrient collection box 4, after the energy storage box 7 transports the nutrient solution inside the measuring component 5, the stirring mechanism 3 stirs the nutrient solution, and drives the collection plate 41 inside the nutrient collection box 4 to rotate, thereby changing the connectivity between the first one-way valve and other nutrient solution connecting ports, thereby realizing the function of replacing, extracting and transporting multiple nutrient solutions.

[0105] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0106] Obviously, the embodiments described above are only some embodiments of the present invention, rather than all embodiments. The preferred embodiments of the present invention are given in the accompanying drawings, but they do not limit the patent scope of the present invention. The present invention can be implemented in many different forms. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive. Although the present invention has been described in detail with reference to the aforementioned embodiments, for those skilled in the art, it is still possible to modify the technical solutions recorded in the aforementioned specific embodiments, or to perform equivalent replacements for some of the technical features therein. Any equivalent structure made using the contents of the specification and drawings of the present invention, directly or indirectly used in other related technical fields, is also within the scope of patent protection of the present invention.

Claims

1. A synthesis device for semaglutide, characterized in that: The invention comprises a synthesis box (1), wherein a culture medium (2) is provided in the middle of the inner side of the synthesis box (1), a stirring mechanism (3) is provided above the culture medium (2), the stirring mechanism (3) is located on the inner wall of the synthesis box (1), the output end of the stirring mechanism (3) is located on the inner side of the culture medium (2), a nutrient collection box (4) is provided on one side of the stirring mechanism (3), a measuring component (5) is connected to one side of the bottom surface of the nutrient collection box (4), an extraction and conveying part (6) is connected to the bottom of the measuring component (5), and the extraction and conveying part (6) provides suction force to the inside of the measuring component (5) when the extraction and conveying part (6) is in a started state, an energy storage box (7) is provided on the upper side of one end of the extraction and conveying part (6) away from the measuring component (5), the side of the energy storage box (7) away from the extraction and conveying part (6) is connected to the inner side of the nutrient collection box (4), and the energy storage box (7) provides high-pressure air to the inside of the nutrient collection box (4) when the energy storage box (7) is in a started state.

2. A semaglutide synthesis device according to claim 1, characterized in that: A liquid storage tank (11) is provided on the inner side of the front of the synthesis box (1), and nutrient bottles (14) are placed in sequence on the inner side of the liquid storage tank (11). A control panel (13) is provided directly above the liquid storage tank (11), and the control panel (13) is fixed on the top surface of the synthesis box (1). A synthesis chamber (12) is provided on the inner side of the liquid storage tank (11), and the culture medium (2) is located at the inner bottom of the synthesis chamber (12). The stirring mechanism (3) is located on the inner top surface of the synthesis chamber (12). A collecting hopper (21) is provided on one side of the top of the culture medium (2), and the collecting hopper (21) is located directly below the measuring component (5). The collecting hopper (21) is connected to the inner side of the culture medium (2). A purification box (22) is provided on the side of the culture medium (2) away from the collecting hopper (21), and the purification box (22) is connected to the bottom of the outer wall of the collecting hopper (21).

3. A semaglutide synthesis device according to claim 2, characterized in that: The stirring mechanism (3) comprises a driving motor (31), the driving motor (31) being fixed on the inner side of the top surface of the synthesis chamber (12), the output end of the driving motor (31) being connected to a driving gear (32), the bottom surface of the driving gear (32) being connected to a driving shaft (33), the bottom end of the driving shaft (33) being connected to a stirring plate (34), the bottom surface of the stirring plate (34) being connected to annular stirring rods (35) equidistantly distributed, the stirring rods (35) being inserted into the culture medium (2 ) inside, a speed change gear (36) is provided on the side of the driving gear (32) close to the nutrient collection box (4), the speed change gear (36) is rotatably connected to the top surface of the synthesis chamber (12), the speed change gear (36) is meshed with the driving gear (32), the bottom surface of the speed change gear (36) is connected to a transmission pulley (37), a transmission belt (38) is sleeved on the outside of the transmission pulley (37), and the other end of the transmission belt (38) is transmission sleeved with the nutrient collection box (4).

4. A semaglutide synthesis device according to claim 3, characterized in that: The nutrient collection box (4) comprises a collection plate (41), the outer side of the top surface of the collection plate (41) is fixed with annularly distributed fixing rods (42), the other end of the fixing rods (42) is fixed to the inner top surface of the synthesis chamber (12), the inner side of the collection plate (41) is provided with a collection groove (43), the inner top of the collection groove (43) is fitted with an adjustment plate (46) for sliding sealing, the center of the top surface of the adjustment plate (46) is fixed with an adjustment shaft (45), the other end of the adjustment shaft (45) is rotatably connected to the inner side of the inner top surface of the synthesis chamber (12), the outer side of the top surface of the collection plate (41) is connected with annularly equidistantly distributed nutrient tubes (44), one end of the nutrient tubes (44) connecting the collection plate (41) with the inner connection of the collection groove (43) The other ends of the nutrition tubes (44) are respectively inserted into the inner sides of a plurality of nutrition bottles (14) inside the liquid storage tank (11); a first one-way valve (47) is provided inside the adjustment disk (46) and located below a single nutrition tube (44); the top of the first one-way valve (47) is communicated with the inside of the nutrition tube (44); the bottom of the first one-way valve (47) is communicated with the lower inner side of the collecting tank (43); a driven pulley (48) is fixed to the top of the outer wall of the adjustment shaft (45); one end of the transmission belt (38) away from the transmission pulley (37) is sleeved on the outer side of the driven pulley (48); a liquid outlet (49) is provided on one side of the bottom surface of the collecting disk (41); the bottom of the liquid outlet (49) is communicated with the inner side of the measuring component (5).

5. The synthesis device of semaglutide according to claim 4, characterized in that: The first one-way valve (47) includes a valve stem (471), both ends of the valve stem (471) are fixed to the inner wall of the adjusting disk (46), symmetrical valve plates (472) are provided on both sides of the middle of the valve stem (471), a hinge rod (473) is fixedly connected to the inner side of one end of the valve plate (472) close to the valve stem (471), a return spring (474) is sleeved on the outer side of the hinge rod (473), the position where the hinge rod (473) sleeves the return spring (474) is hinged to the inner side of the valve stem (471), and both ends of the return spring (474) are respectively fixed to the outer wall of the hinge rod (473) and the inner wall of the valve stem (471).

6. The synthesis device of semaglutide according to claim 5, characterized in that: The measuring assembly (5) comprises a measuring tube (51), the top end of which is fixed to the bottom surface of the nutrient collection box (4), a measuring cylinder (53) arranged in an annular manner is provided in the middle of the inner side of the measuring tube (51), a liquid collecting groove (52) is provided at the upper and lower ends of the measuring cylinder (53), the top of the liquid collecting groove (52) is connected to the liquid outlet (49), the bottom of the liquid collecting groove (52) is connected to the measuring cylinder (53), a liquid spraying nozzle (54) is provided on one side of the bottom of the measuring tube (51), the liquid spraying nozzle (54) is connected to the inside of the liquid collecting groove (52), a dripping cone block (55) is provided below the liquid spraying nozzle (54), and the dripping cone block (55) is fixed to the bottom surface of the measuring tube (51).

7. The synthesis device of semaglutide according to claim 1, characterized in that: The extraction and conveying part (6) includes an extraction tube (61), a sealing plug (62) is slidably fitted on the inner side of the extraction tube (61), and a pulling screw (63) is connected to the center of one side of the sealing plug (62) for limited rotation. A driving motor (64) is fixed to the outer wall of the end of the extraction tube (61) away from the sealing plug (62), and a driving screw (65) is connected to the output end of the driving motor (64), and the driving screw (65) is meshed with the driving motor (64). The end of the extraction tube (61) connected to the sealing plug (62) is connected to a pneumatic tube (69), and the end of the pneumatic tube (69) away from the extraction tube (61) is connected to the inner side of the liquid collecting tank (52).

8. The semaglutide synthesis device according to claim 7, characterized in that: The side of the driving screw rod (65) away from the driving motor (64) is connected to an energy storage driving rod (66), the outer surface of the energy storage driving rod (66) is sleeved with an energy storage pulley (67), the outer side of the energy storage pulley (67) is sleeved with an energy storage transmission belt (68), and the other end of the energy storage transmission belt (68) is transmission-connected to the energy storage box (7).

9. The semaglutide synthesis device according to claim 8, characterized in that: An energy storage block (71) is slidably connected to the inner side of the energy storage box (7). A vent (72) with two ends passing through is provided at the center of the energy storage block (71). A second one-way valve (73) is provided on the inner side of the top of one end of the vent (72). An air outlet pipe (74) is connected to the top of the end of the outer wall of the energy storage box (7) connected to the second one-way valve (73). A third one-way valve (75) is fixed to the position of the energy storage box (7) connected to the air outlet pipe (74). The end of the air outlet pipe (74) away from the energy storage box (7) is connected to the inner side of the liquid outlet (49). Symmetrical one-way elastic stoppers (75) are fixed to the outer walls of both sides of the energy storage block (71). 8), one end of the one-way elastic stopper (78) elastically slides inside the energy storage block (71) in a limited manner, the other end of the one-way elastic stopper (78) penetrates the outer wall of the energy storage box (7) and extends to the outside of the energy storage box (7), the end of the one-way elastic stopper (78) located on the outside of the energy storage box (7) slides inside the energy storage box (7), a positioning rod (76) is fixed at a position of the outer wall of the energy storage box (7) close to the one-way elastic stopper (78), symmetrical tension springs (79) are fixed on both sides of the bottom of one end of the energy storage box (7) close to the second one-way valve (73), and the other end of the tension spring (79) is fixed to the outer wall of the energy storage block (71).

10. The semaglutide synthesis device according to claim 9, characterized in that: An energy storage transmission part (77) is provided below the energy storage box (7), and the energy storage transmission part (77) comprises an energy storage transmission shaft (771), and both ends of the energy storage transmission shaft (771) are rotatably connected to the inner wall of the synthesis chamber (12). Energy storage wheels (772) are fixed to the outer walls of both sides of the energy storage transmission shaft (771) located below the energy storage box (7), and a belt pulley (773) is provided between the energy storage wheels (772). The belt pulley (773) is fixedly sleeved on the outer wall of the energy storage transmission shaft (771), and one end of the energy storage transmission belt (68) away from the energy storage belt pulley (67) is sleeved on the outer side of the belt pulley (773). An energy storage toggle rod (774) is fixed on the upper side of the outer wall of the energy storage wheel (772), and the energy storage toggle rod (774) is inserted between the positioning rod (76) and the one-way elastic stopper (78).

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