Phage fermentation equipment with sterile foam discharging function and fermentation process thereof

Abstract

The invention provides bacteriophage fermentation equipment with a sterile foam discharging function and a fermentation process of the bacteriophage fermentation equipment, and relates to the technical field of ferment.The bacteriophage fermentation equipment comprises a fermentation tank body and a sealing cover body, the sealing cover body is provided with a foam compression cylinder body and a foam conveying channel, and a piston is slidably installed in the foam compression cylinder body; a foam feeding channel and a foam discharging channel are arranged on the foam compression cylinder body, a foam feeding valve and a foam discharging valve are correspondingly arranged at the foam feeding channel and the foam discharging channel, the foam feeding valve and the foam discharging valve are in transmission connection with a cam shaft driving mechanism, and the foam feeding channel is communicated with the foam conveying channel; a vertical foam conveying assembly and a transverse foam conveying assembly are rotationally mounted on the inner side of the foam conveying channel, pressure reduction and pressurization are conducted on the interior of the foam compression cylinder body through a piston, opening or closing of a foam inlet valve and a foam discharging valve is matched, and therefore the foam suction action, the compression foam breaking action and the one-way foam discharging action of the foam compression cylinder body are achieved; therefore, one-way and continuous discharge of foam is realized, and sterile foam removal of fermentation liquor in the fermentation tank body is ensured.

Description

Technical Field

[0001] The invention relates to the technical field of fermentation, in particular to bacteriophage fermentation equipment with aseptic foam discharge function and a fermentation process thereof. Background Art

[0002] During the phage fermentation process, gas is produced, and the gas forms bubbles in the fermentation liquid. The bubbles rise to the top of the fermentation liquid and form foam on the top of the fermentation liquid. A large amount of foam gathered above the fermentation liquid will not only hinder the transfer of oxygen, affect the normal respiration of the bacteria in the fermentation liquid, and reduce the fermentation efficiency, but also in order to accommodate the foam and prevent overflow, the charging factor of the fermenter will be reduced, thereby reducing the equipment utilization rate. Therefore, it is necessary to perform a defoaming operation inside the fermenter, and at the same time, it is necessary to ensure that the air inside the tank does not contact the air outside the tank. Mechanical defoaming and chemical defoaming are commonly used to clean the foam in the prior art. Mechanical defoaming is to break the foam with the help of mechanical force, and the foam in the tank is not discharged. Chemical defoaming is to add a defoaming agent to the fermentation liquid to eliminate the foam. If the selection is inappropriate, it will affect the growth of the bacteria or the generation of metabolites, and increase the chance of infection in operation.

[0003] Therefore, there is a need for a bacteriophage fermentation device and a fermentation process thereof with a sterile foam discharge function that can solve the above-mentioned problems. Summary of the invention

[0004] The present invention proposes a bacteriophage fermentation device with a sterile foam discharge function and a fermentation process thereof. The interior of a foam compression cylinder is decompressed and pressurized by a piston, and a foam inlet valve and a foam discharge valve are opened or closed in coordination, thereby realizing the foam compression cylinder's foam absorption, foam compression and foam breaking, and unidirectional foam discharge, thereby realizing unidirectional and continuous discharge of foam, and ensuring the sterile foam removal of the fermentation tank.

[0005] The technical solution of the present invention is achieved in this way: The bacteriophage fermentation equipment with aseptic foam removal function comprises a fermentation tank body, the top of which is provided with a sealing cover body, and the sealing cover body is provided with: A foam compression cylinder, a piston is slidably mounted in the foam compression cylinder, the piston is connected to a crankshaft driving mechanism, a foam inlet channel and a foam discharge channel are provided on the foam compression cylinder, a foam inlet valve and a foam discharge valve are correspondingly provided at the foam inlet channel and the foam discharge channel, the foam inlet valve and the foam discharge valve are drivingly connected to a camshaft driving mechanism, and the camshaft driving mechanism is used to drive the foam inlet valve and the foam discharge valve to open alternately and close synchronously; A foam conveying channel, wherein the foam conveying channel is vertically arranged on the inner side of the sealing cover body, the foam inlet channel is connected to the foam conveying channel, and a foam vertical conveying component and a foam horizontal conveying component are rotatably installed on the inner side of the foam conveying channel from bottom to top in sequence, and the foam horizontal conveying component corresponds to the foam inlet channel.

[0006] As a preferred technical solution, a foam compression and crushing chamber is formed between the foam compression cylinder, the piston, the foam inlet valve and the foam outlet valve.

[0007] As a preferred technical solution, the camshaft driving mechanism includes a driving shaft rotatably mounted on the sealing cover body, and two cams are fixedly mounted on the driving shaft, and the angle between the axes of symmetry of the two cams is 180 degrees and they are symmetrically arranged along the driving shaft, the foam inlet valve includes a foam inlet block, and the foam discharge valve includes a foam discharge block, and the two cams correspond one-to-one to the foam inlet block and the foam discharge block.

[0008] As a preferred technical solution, the foam inlet block is arranged at the connecting port of the foam inlet channel and the foam compression chamber, and a foam inlet driving rod is slidably installed on the foam inlet channel, one end of the foam inlet driving rod extends into the foam inlet channel and is fixedly connected to the foam inlet block, and a reset element is arranged between the other end of the foam inlet driving rod and the foam inlet channel, and the end portion abuts against the outer peripheral surface of the corresponding cam.

[0009] As a preferred technical solution, the foam discharge block is arranged at the connecting port between the foam discharge channel and the foam compression chamber, and a foam discharge driving rod is slidably installed on the foam discharge channel, one end of the foam discharge driving rod extends into the foam discharge channel and is fixedly connected to the foam discharge block, and the reset element is arranged between the other end of the foam discharge driving rod and the foam discharge channel, and the end portion abuts against the outer peripheral surface of the corresponding cam.

[0010] As a preferred technical solution, the crankshaft drive mechanism includes two connecting rods rotatably mounted on the sealing cover body, one of the connecting rods is drivingly connected to the drive shaft, a first motor is fixedly mounted on the sealing cover body, the first motor is drivingly connected to the other connecting rod, a crank arm is fixedly mounted on one end of the two connecting rods which are opposite to each other, an eccentric rod which is eccentrically mounted is fixedly connected between the two crank arms, and the piston rod on the piston is hinged to the eccentric rod.

[0011] As a preferred technical solution, the foam vertical conveying component includes foam spiral vertical conveying blades, and the foam transverse conveying component includes a plurality of foam discharge transverse pushing blades. A rotating shaft is rotatably installed in the fermentation tank body, and the rotating shaft is vertically arranged. The foam spiral vertical conveying blades and the foam discharge transverse pushing blades are fixedly installed on the rotating shaft.

[0012] As a preferred technical solution, a plurality of stirring blades and a plurality of conical defoaming paddles are fixedly mounted on the rotating shaft. The conical defoaming paddles and the stirring blades are arranged from bottom to top below the foam spiral vertical conveying blades. The conical defoaming paddles are all horizontally arranged cylinders, and each of the cylinders is a cone with openings at both ends. Each of the conical defoaming paddles is fixedly mounted on the rotating shaft by a horizontally arranged mounting rod.

[0013] The fermentation process of the bacteriophage fermentation equipment with aseptic foam removal function specifically comprises the following steps: S1, the fermentation liquid is fermented in the sealed space formed by the fermentation tank body and the sealing cover body, and the foam formed by the fermentation is accumulated above the fermentation liquid; S2. When it is necessary to discharge foam from the fermentation tank, the foam vertical conveying component and the foam horizontal conveying component convey the foam toward the foam inlet channel; S3, before foam suction, the foam inlet valve is in an open state, the foam discharge valve is in a closed state, the piston moves toward the outside of the foam compression cylinder, the pressure in the foam compression cylinder decreases, and part of the foam in the foam delivery channel is adsorbed into the foam compression cylinder by negative pressure, that is, the foam suction action of the foam compression cylinder is completed; S4, after the foam suction action is completed, the foam inlet valve is closed, the piston moves toward the inner side of the foam compression cylinder, and the foam is compressed and broken, that is, the compression and foam breaking action is completed; S5. After the foam breaking action is completed, at this time, the foam inlet valve is in a closed state, and the foam discharge valve is opened. The liquid formed after the foam breaks is discharged from the foam discharge channel, completing the one-way foam discharge action, that is, completing one aseptic foam discharge, and so on, the foam inside the fermentation tank body can be continuously discharged.

[0014] By adopting the above technical solution, the beneficial effects of the present invention are as follows: Since the bacteriophage fermentation equipment with aseptic foam discharge function and the fermentation process thereof include a foam compression cylinder and a foam discharge channel, during the use of the equipment, the fermentation liquid is fermented in a sealed space formed by the fermentation tank body and the sealing cover body, and when it is necessary to discharge the foam on the upper layer of the fermentation liquid, the foam vertical conveying component on the inner side of the foam conveying channel conveys the foam on the upper layer of the fermentation liquid to the direction of the foam transverse conveying component, and the foam transverse conveying component then conveys the foam to the direction of the foam inlet channel. At the same time, the foam inlet valve is in an open state, the foam discharge valve is in a closed state, the piston moves toward the outside of the foam compression cylinder, the pressure in the foam compression cylinder is reduced, and part of the foam in the foam delivery channel is adsorbed into the foam compression cylinder by negative pressure, that is, the foam compression cylinder foam suction action is realized, after the foam suction action is completed, the foam inlet valve is closed, the piston moves toward the inside of the foam compression cylinder, the foam is compressed and broken, that is, the compression and foam breaking action is realized, after the foam breaking action is completed, the foam discharge valve is opened, and the liquid formed after the foam is broken is discharged from the foam discharge channel, and at this time the foam inlet valve is in a closed state, that is, the one-way foam discharge action is realized.

[0015] In the present invention, the interior of the foam compression cylinder is decompressed and pressurized by a piston, and the foam inlet valve and the foam discharge valve are opened or closed in coordination, thereby realizing the foam compression cylinder's foam absorption, foam compression and foam breaking, and unidirectional foam discharge, thereby realizing unidirectional and continuous discharge of foam, and ensuring aseptic foam removal of the fermentation liquid in the fermenter.

[0016] Compared with the existing mechanical defoaming, the mechanical defoaming reintroduces the liquid gathered by the foam into the tank body, which will cause secondary pollution. The mechanical defoaming method can only eliminate part of the foam, especially when the amount of foam is large and the foam generation density is high, the defoaming effect is not ideal. The present invention discharges the foam unidirectionally and continuously, avoiding contamination of the fermentation liquid. The continuous discharge of foam avoids the accumulation of foam and has a good foam cleaning effect.

[0017] Since the bacteriophage fermentation equipment with aseptic foam discharge function and its fermentation process include a foam compression cylinder, during the aseptic foam discharge process, the end of the two cams close to the driving shaft is the proximal end of the cam, and the other end away from the driving shaft is the distal end of the cam. When the foam inlet driving rod and the foam discharge driving rod respectively abut against the vicinity of the corresponding proximal ends of the cams, the foam inlet block closes the connecting port connecting the foam inlet channel and the foam compression chamber, that is, the foam inlet valve is in a closed state, and the foam discharge block closes the connecting port connecting the foam discharge channel and the foam compression chamber, that is, the foam discharge valve is in a closed state.

[0018] When the foam compression cylinder performs the foam suction action, with the rotation of the driving shaft, the foam inlet driving rod abuts against the distal end of the corresponding cam, that is, the foam inlet block opens the connecting port connecting the foam inlet channel and the foam compression chamber, and because the angle between the symmetry axes of the two cams is 180 degrees and they are symmetrically arranged along the driving shaft, the foam discharge driving rod at this time abuts against the proximal end of the corresponding cam, that is, the foam discharge block closes the connecting port connecting the foam discharge channel and the foam compression chamber, thereby realizing the opening of the foam inlet valve and the closing of the foam discharge valve during foam suction, thereby avoiding the contact between the air in the fermentation tank and the air outside the fermentation tank during foam suction, thereby realizing aseptic foam suction.

[0019] When the foam compression cylinder compresses and breaks the foam, with the rotation of the driving shaft, the foam inlet driving rod and the foam discharge driving rod again abut against the proximal end of the corresponding cam, that is, the foam inlet valve and the foam discharge valve are in a closed state at the same time. At this time, a foam compression and crushing chamber is formed between the foam compression cylinder, the piston, the foam inlet valve and the foam discharge valve. As the piston continuously compresses the air in the foam compression and crushing chamber, the foam is crushed.

[0020] When the foam compression cylinder is performing the foam discharging action, as the driving shaft rotates, the foam discharging driving rod abuts against the distal end of the corresponding cam, that is, the foam discharging block opens the connecting port connecting the foam discharging channel and the foam compression chamber, and because the angle between the symmetry axes of the two cams is 180 degrees and they are symmetrically arranged along the driving shaft, the foam inlet driving rod at this time abuts against the proximal end of the corresponding cam, that is, the foam inlet block closes the connecting port connecting the foam inlet channel and the foam compression chamber, thereby realizing the closing of the foam inlet valve and the opening of the foam discharging valve during foam discharging, thereby avoiding the contact between the air in the fermentation tank and the air outside the fermentation tank during foam discharging, thereby realizing aseptic foam discharging.

[0021] In the present invention, the movement of the foam inlet block and the foam discharge block is realized by the cooperation of the foam inlet driving rod, the foam discharge driving rod and the two cams, thereby realizing the alternate opening and synchronous closing of the foam inlet valve and the foam discharge valve.

[0022] Since the rotating shaft is also fixedly installed with a stirring blade and a conical defoaming paddle, during the use of the equipment, as the rotating shaft rotates, the stirring blade, the conical defoaming paddle and the mounting rod will stir the fermentation liquid, and some bubbles will move to the upper layer of the fermentation liquid to form foam on the top of the fermentation liquid. The conical defoaming paddle can puncture some of the bubbles in the fermentation liquid. At the same time, the cylindrical structure can also reduce the entry of air into the fermentation liquid, thereby achieving defoaming of the fermentation liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0024] Figure 1 This is a reference diagram of the state of the foam compression cylinder when foam is entering; Figure 2 This is a reference diagram of the state of the foam compression cylinder when discharging foam; Figure 3 It is a structural schematic diagram of a sealing cover; Figure 4 It is a schematic diagram of the structure of two cams.

[0025] Wherein: 1. fermentation tank body; 2. sealing cover body; 3. foam compression cylinder body; 4. piston; 5. foam inlet channel; 6. foam discharge channel; 7. foam inlet valve; 8. foam discharge valve; 9. foam conveying channel; 10. foam compression crushing chamber; 11. driving shaft; 12. cam; 13. symmetric axis; 14. foam inlet block; 15. foam discharge block; 16. foam inlet driving rod; 17. compression spring; 18. foam discharge driving rod; 19. connecting rod; 20. crank arm; 21. eccentric rod; 22. foam spiral vertical conveying blade; 23. foam discharge horizontal pushing blade; 24. rotating shaft; 25. stirring blade; 26. conical defoaming paddle; 27. mounting rod; 28. bolt; 29. ​​liquid discharge port; 30. liquid inlet port; 31. valve; 32. first motor; 33. second motor; 34. synchronous pulley; 35. synchronous belt. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] like Figure 1-Figure 4 As shown in the figure, the bacteriophage fermentation equipment with aseptic foam discharge function includes a fermentation tank body 1, a sealing cover body 2 is arranged on the top of the fermentation tank body 1, and the sealing cover body 2 is provided with: A foam compression cylinder 3 is provided with a piston 4 slidably mounted in the foam compression cylinder 3, the piston 4 is connected to a crankshaft driving mechanism, a foam inlet channel 5 and a foam discharge channel 6 are provided on the foam compression cylinder 3, the foam discharge channel 6 is communicated with the outside of the fermentation tank body 1, a foam inlet valve 7 and a foam discharge valve 8 are provided at the foam inlet channel 5 and the foam discharge channel 6 respectively, the foam inlet valve 7 and the foam discharge valve 8 are transmission-connected with a camshaft driving mechanism, and the camshaft driving mechanism is used to drive the foam inlet valve 7 and the foam discharge valve 8 to open alternately and close synchronously.

[0028] The foam conveying channel 9 is vertically arranged on the inner side of the sealing cover body 2, and the foam inlet channel 5 is connected to the foam conveying channel 9. The inner side of the foam conveying channel 9 is rotated from bottom to top with a foam vertical conveying component and a foam horizontal conveying component corresponding to the foam inlet channel 5.

[0029] A foam compression crushing chamber 10 is formed between the foam compression cylinder 3 , the piston 4 , the foam inlet valve 7 and the foam outlet valve 8 .

[0030] like Figure 3 and Figure 4 As shown together, the camshaft driving mechanism includes a driving shaft 11 rotatably mounted on the sealing cover body 2, and two cams 12 are fixedly mounted on the driving shaft 11. The angle between the symmetry axes 13 of the two cams 12 is 180 degrees and they are symmetrically arranged along the driving shaft 11. The foam inlet valve 7 includes a foam inlet block 14, and the foam discharge valve 8 includes a foam discharge block 15. The two cams 12 correspond to the foam inlet block 14 and the foam discharge block 15 one by one.

[0031] In addition, a foam inlet block 14 is arranged at the connecting port where the foam inlet channel 5 is connected to the foam compression chamber, and a foam inlet driving rod 16 is slidably installed on the foam inlet channel 5. One end of the foam inlet driving rod 16 extends into the foam inlet channel 5 and is fixedly connected to the foam inlet block 14, and a reset element is arranged between the other end of the foam inlet driving rod 16 and the foam inlet channel 5, and the end portion abuts against the outer peripheral surface of the corresponding cam 12.

[0032] In addition, a foam discharge block 15 is arranged at the connecting port between the foam discharge channel 6 and the foam compression chamber, and a foam discharge driving rod 18 is slidably installed on the foam discharge channel 6. One end of the foam discharge driving rod 18 extends into the foam discharge channel 6 and is fixedly connected to the foam discharge block 15. A reset element is arranged between the other end of the foam discharge driving rod 18 and the foam discharge channel 6, and the end portion abuts against the outer peripheral surface of the corresponding cam 12.

[0033] In the present invention, the reset element comprises a compression spring 17, which can quickly reset the foam inlet block 14 and the foam discharge block 15, thereby quickly closing the foam inlet channel 5 and the foam discharge channel 6 respectively.

[0034] Secondly, the crankshaft drive mechanism includes two connecting rods 19 rotatably mounted on the sealing cover body 2, one of the connecting rods 19 is transmission-connected to the drive shaft 11, a first motor 32 is fixedly mounted on the sealing cover body 2, the first motor 32 is transmission-connected to the other connecting rod 19, a crank arm 20 is fixedly mounted on one end of the two connecting rods 19 which are arranged opposite to each other, an eccentric rod 21 which is eccentrically mounted is fixedly connected between the two crank arms 20, and the piston rod on the piston 4 is hinged to the eccentric rod 21.

[0035] The foam vertical conveying component includes a foam spiral vertical conveying blade 22, and the foam horizontal conveying component includes a plurality of foam discharge horizontal pushing blades 23. A rotating shaft 24 is rotatably installed in the fermentation tank body 1, and the rotating shaft 24 is vertically arranged. The foam spiral vertical conveying blade 22 and the foam discharge horizontal pushing blade 23 are both fixedly installed on the rotating shaft 24.

[0036] like Figure 3 As shown, a plurality of stirring blades 25 and a plurality of conical defoaming paddles 26 are fixedly mounted on the rotating shaft 24. The conical defoaming paddles 26 and the stirring blades 25 are arranged from bottom to top below the foam spiral vertical conveying blades 22. The conical defoaming paddles 26 are all horizontally arranged cylinders, and each cylinder is a cone with openings at both ends. Each conical defoaming paddle 26 is fixedly mounted on the rotating shaft 24 by a horizontally arranged mounting rod 27.

[0037] The sealing cover 2 is fixedly mounted on the open top of the fermentation tank body 1 by bolts 28 .

[0038] A liquid discharge port 29 is provided at the bottom of the fermentation tank body 1 , and a liquid inlet 30 is provided on the sealing cover body 2 . A valve 31 is provided at both the liquid discharge port 29 and the liquid inlet 30 .

[0039] A second motor 33 is fixedly mounted on the sealing cover body 2 , and the second motor 33 is transmission-connected to the rotating shaft 24 .

[0040] A synchronous pulley 34 is fixedly mounted on the connecting rod 19 and the driving shaft 11 , respectively, and a synchronous belt 35 is wound between the two synchronous pulleys 34 .

[0041] The fermentation process of the bacteriophage fermentation equipment with aseptic foam discharge function specifically includes the following steps: S1. The fermentation liquid is fermented in the sealed space formed by the fermentation tank body 1 and the sealing cover body 2, and the foam formed by the fermentation accumulates above the fermentation liquid.

[0042] S2. When it is necessary to discharge foam from the fermentation tank body 1, the foam vertical conveying assembly and the foam horizontal conveying assembly convey the foam toward the foam inlet channel 5.

[0043] S3. Before foam suction, the foam inlet valve 7 is in an open state, the foam discharge valve 8 is in a closed state, the piston 4 moves toward the outside of the foam compression cylinder 3, the pressure in the foam compression cylinder 3 decreases, and part of the foam in the foam delivery channel 9 is adsorbed into the foam compression cylinder 3 by the negative pressure, thus completing the foam suction action of the foam compression cylinder 3.

[0044] S4, after the foam suction action is completed, the foam inlet valve 7 is closed, the piston 4 moves toward the inside of the foam compression cylinder 3, and the foam is compressed and broken, that is, the compression and foam breaking action is completed.

[0045] S5, after the foam breaking action is completed, at this time, the foam inlet valve 7 is in a closed state, and the foam discharge valve 8 is opened. The liquid formed after the foam is broken is discharged from the foam discharge channel 6, completing the one-way foam discharge action, that is, completing a sterile foam discharge, and so on, the foam inside the fermentation tank body 1 can be continuously discharged.

[0046] The working process of the present invention is as follows: In the first step, during the use of the present device, a sealed fermentation space is formed inside the fermentation tank body 1 and the sealing cover body 2, and the fermentation liquid is located in the fermentation tank body 1 and fermented. When it is necessary to discharge foam from the fermentation tank body 1, the second motor 33 drives the rotating shaft 24 to rotate, and the stirring blades 25, the conical defoaming paddle 26 and the mounting rod 27 stir the fermentation liquid, and the bubbles in the fermentation liquid move upward to form foam on the surface of the fermentation liquid.

[0047] In the second step, the foam spiral vertical conveying blade 22 conveys the foam toward the foam discharge lateral pushing blade 23 , and the foam discharge lateral pushing blade 23 conveys the foam toward the foam inlet channel 5 .

[0048] In the third step, driven by the first motor 32 , the foam inlet valve 7 is opened, the piston 4 moves toward the outside of the foam compression cylinder 3 , the pressure in the foam compression and crushing chamber 10 is reduced, and the foam in the foam conveying channel 9 enters the foam compression and crushing chamber 10 through the foam inlet channel 5 .

[0049] In the fourth step, driven by the first motor 32 , the foam inlet valve 7 is closed, the piston 4 moves toward the inside of the foam compression cylinder 3 , the pressure in the foam compression and crushing chamber 10 increases, and the foam in the foam compression and crushing chamber 10 is crushed.

[0050] In the fifth step, the foam discharge valve 8 is opened, and the liquid formed after the foam is broken is discharged from the foam discharge channel 6. After the liquid is discharged, the foam discharge valve 8 is closed, and an aseptic foam discharge action is completed. This reciprocating process can continuously discharge the foam in the fermentation tank body 1.

[0051] In summary, the present invention reduces the pressure and increases the pressure inside the foam compression cylinder through the piston, and cooperates with the opening or closing of the foam inlet valve and the foam discharge valve, thereby realizing the foam compression cylinder's foam absorption, foam compression and one-way foam discharge, and further realizing the one-way and continuous discharge of foam, ensuring the aseptic defoaming of the fermentation liquid in the fermenter.

[0052] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A bacteriophage fermentation device with aseptic foam removal function, comprising a fermentation tank body, a sealing cover body is provided on the top of the fermentation tank body, characterized in that: The sealing cover is provided with: A foam compression cylinder, a piston is slidably mounted in the foam compression cylinder, the piston is connected to a crankshaft driving mechanism, a foam inlet channel and a foam discharge channel are provided on the foam compression cylinder, a foam inlet valve and a foam discharge valve are correspondingly provided at the foam inlet channel and the foam discharge channel, the foam inlet valve and the foam discharge valve are drivingly connected to a camshaft driving mechanism, and the camshaft driving mechanism is used to drive the foam inlet valve and the foam discharge valve to open alternately and close synchronously; A foam conveying channel, wherein the foam conveying channel is vertically arranged on the inner side of the sealing cover body, the foam inlet channel is connected to the foam conveying channel, and a foam vertical conveying component and a foam horizontal conveying component are rotatably installed on the inner side of the foam conveying channel from bottom to top in sequence, and the foam horizontal conveying component corresponds to the foam inlet channel.

2. The bacteriophage fermentation equipment with aseptic foam discharge function according to claim 1, characterized in that: A foam compression and crushing chamber is formed between the foam compression cylinder, the piston, the foam inlet valve and the foam discharge valve.

3. The bacteriophage fermentation equipment with aseptic foam discharge function according to claim 1, characterized in that: The camshaft driving mechanism includes a driving shaft rotatably mounted on the sealing cover body, and two cams are fixedly mounted on the driving shaft. The angle between the symmetry axes of the two cams is 180 degrees and the two cams are symmetrically arranged along the driving shaft. The foam inlet valve includes a foam inlet block, and the foam discharge valve includes a foam discharge block. The two cams correspond one to one with the foam inlet block and the foam discharge block.

4. The bacteriophage fermentation equipment with aseptic foam discharge function according to claim 3, characterized in that: The foam inlet block is arranged at the connecting port between the foam inlet channel and the foam compression chamber, and a foam inlet driving rod is slidably installed on the foam inlet channel, one end of the foam inlet driving rod extends into the foam inlet channel and is fixedly connected to the foam inlet block, and a reset element is arranged between the other end of the foam inlet driving rod and the foam inlet channel, and the end portion abuts against the outer peripheral surface of the corresponding cam.

5. The bacteriophage fermentation equipment with aseptic foam discharge function according to claim 4, characterized in that: The foam discharge block is arranged at the connecting port between the foam discharge channel and the foam compression chamber, a foam discharge driving rod is slidably installed on the foam discharge channel, one end of the foam discharge driving rod extends into the foam discharge channel and is fixedly connected to the foam discharge block, and the reset element is arranged between the other end of the foam discharge driving rod and the foam discharge channel, and the end portion abuts against the outer peripheral surface of the corresponding cam.

6. The bacteriophage fermentation equipment with aseptic foam discharge function according to claim 3, characterized in that: The crankshaft driving mechanism includes two connecting rods rotatably mounted on the sealing cover body, one of the connecting rods is drivingly connected to the driving shaft, a first motor is fixedly mounted on the sealing cover body, the first motor is drivingly connected to the other connecting rod, a crank arm is fixedly mounted on one end of the two connecting rods that are opposite to each other, an eccentric rod is fixedly connected eccentrically between the two crank arms, and the piston rod on the piston is hinged to the eccentric rod.

7. The bacteriophage fermentation equipment with aseptic foam discharge function according to claim 1, characterized in that: The foam vertical conveying component includes foam spiral vertical conveying blades, and the foam transverse conveying component includes a plurality of foam discharge transverse pushing blades. A rotating shaft is rotatably installed in the fermentation tank body, and the rotating shaft is vertically arranged. The foam spiral vertical conveying blades and the foam discharge transverse pushing blades are fixedly installed on the rotating shaft.

8. The bacteriophage fermentation equipment with aseptic foam discharge function according to claim 7, characterized in that: A plurality of stirring blades and a plurality of conical defoaming paddles are also fixedly mounted on the rotating shaft. The conical defoaming paddles and the stirring blades are arranged from bottom to top below the foam spiral vertical conveying blades. The conical defoaming paddles are all horizontally arranged cylinders, and each of the cylinders is a cone with openings at both ends. Each of the conical defoaming paddles is fixedly mounted on the rotating shaft through a horizontally arranged mounting rod.

9. The fermentation process of the bacteriophage fermentation equipment with aseptic foam discharge function according to any one of claims 1 to 8, characterized in that: The specific steps include: S1, the fermentation liquid is fermented in the sealed space formed by the fermentation tank body and the sealing cover body, and the foam formed by the fermentation is accumulated above the fermentation liquid; S2. When it is necessary to discharge foam from the fermentation tank, the foam vertical conveying component and the foam horizontal conveying component convey the foam toward the foam inlet channel; S3, before foam suction, the foam inlet valve is in an open state, the foam discharge valve is in a closed state, the piston moves toward the outside of the foam compression cylinder, the pressure in the foam compression cylinder decreases, and part of the foam in the foam delivery channel is adsorbed into the foam compression cylinder by negative pressure, that is, the foam suction action of the foam compression cylinder is completed; S4, after the foam suction action is completed, the foam inlet valve is closed, the piston moves toward the inner side of the foam compression cylinder, and the foam is compressed and broken, that is, the compression and foam breaking action is completed; S5. After the foam breaking action is completed, at this time, the foam inlet valve is in a closed state, and the foam discharge valve is opened. The liquid formed after the foam breaks is discharged from the foam discharge channel, completing the one-way foam discharge action, that is, completing one aseptic foam discharge, and so on, the foam inside the fermentation tank body can be continuously discharged.

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