Sample detection device and detection method

Abstract

The invention provides a sample detection device and a detection method, the sample detection device comprises a sampling rod and a piston, a sample treatment liquid is pre-stored above the piston and is in contact with the sampling rod, and when the sampling rod is pulled out for sampling, the sampling rod can automatically extend under the cooperation of a sampling rod sleeve, so that the piston can be pushed away when the sampling is completed and the detection device is inserted; the device is simple in structure, convenient to use and low in cost, the accuracy and precision of detection can be ensured, and a more reliable detection result can be quickly provided.

Description

[0001] This application claims the priority of the Chinese prior application, application number: 202311138617.5, and filing date September 5, 2023. The specification, claims, abstract and set of drawings of that application are all cited as part of this application. Technical Field

[0002] The present invention belongs to the field of medical devices, and in particular relates to a sample detection device and a detection method. Background Art

[0003] Sample testing is an important method in the fields of biomedicine, environmental monitoring, chemical analysis, etc. It usually requires mixing the sample liquid with one or more liquids and then testing it through instruments or test strips. However, most of the existing sample testing devices are large in size and inconvenient to carry. They also require a lot of time and materials to collect, process, transfer and clean samples before and after testing, which is not only inefficient but also easy to cause sample contamination or loss.

[0004] In order to solve the above problems, some portable sample detection devices have been developed. For example, CN111269825A discloses a rapid nucleic acid POCT integrated microfluidic kit and its preparation method and application, which integrates the nucleic acid extraction, amplification and detection functions to achieve closed-tube nucleic acid extraction and amplification detection integration. However, the above portable sample detection device still has some shortcomings: on the one hand, it is necessary to open and close the box or mounting seat and other components many times during the collection and detection process, which may easily lead to leakage or volatilization of the sample liquid or mixed liquid; on the other hand, when the mixed liquid is tested, it needs to be transferred to the instrument or test paper and other components, which not only increases the operation steps and consumables costs, but also may affect the accuracy and sensitivity of the detection.

[0005] Therefore, in view of the need for testing the mixture of sample liquid and sample processing liquid, it is urgent to develop an integrated, closed, portable sample detection device to solve the problems in the prior art. Summary of the invention

[0006] To solve the above problems, the present invention provides a sample detection device and a detection method, including a sampling rod and a piston. The sample processing liquid is pre-stored above the piston and contacts the sampling rod. When the sampling rod is pulled out for sampling, it can be automatically extended with the cooperation of the sampling rod sleeve, so that when the sampling is completed and the detection device is inserted, the piston can be pushed open, so that the sample processing liquid containing the sample to be tested flows downward and contacts the sample detection component, thereby completing the detection. The device has a simple structure, is easy to use, and has low cost. It can also ensure the accuracy and precision of the detection, and quickly provide more reliable detection results.

[0007] In one aspect, the present invention provides a sample detection device, the device comprising a sample collection component, a sample processing component and a sample detection component; the sample processing component is filled with a sample processing liquid and a piston, and the piston is used to isolate the sample processing liquid and the sample detection component;

[0008] The sample collection component includes a sampling rod, which can be extended and push the piston after sampling is completed, so that the sample liquid to be tested contacts the sample detection component;

[0009] The sample detection component is used to detect the sample liquid to be tested.

[0010] The sample detection device provided by the present invention cleverly realizes that the sampling rod can be extended after sampling is completed by designing the structure of the sample collection component, thereby automatically pushing open the piston, allowing the sample processing liquid to flow downward and contact the sample detection component, thereby realizing automatic control of the liquid in the device when the device is fully closed.

[0011] Furthermore, the sampling rod is also covered with a sampling rod cover, and the sampling rod is provided with a first limiting mechanism and a second limiting mechanism in sequence from bottom to top. In the initial state, the lower end of the sampling rod cover is combined with the first limiting mechanism. After the sampling rod completes sampling, the lower end of the sampling rod cover is separated from the first limiting mechanism and combined with the second limiting mechanism, thereby extending the sampling rod and pushing the piston.

[0012] The present invention designs the structure of the sampling rod and the sampling rod cover so that when the sampling rod is pulled out of the detection device for sampling, the sampling rod cover can change from a state of being combined with the first limiting mechanism to a state of being combined with the second limiting mechanism, and once the sampling rod cover leaves the first limiting mechanism, it is difficult to recover and cannot return to the position of the first limiting mechanism.

[0013] Furthermore, both the first limiting mechanism and the second limiting mechanism have a section of structure that gradually thickens from bottom to top, and there is a groove at the connection between the first limiting mechanism and the second limiting mechanism, where the cross-section of the sampling rod suddenly decreases from large to small; the maximum size of the second limiting mechanism is larger than the maximum size of the first limiting mechanism.

[0014] The suddenly reduced groove forms a platform structure at the connection between the first limiting mechanism and the second limiting mechanism, and the platform structure can prevent the sampling rod sleeve from returning to the first limiting mechanism.

[0015] In some embodiments, the maximum size of the first limiting mechanism needs to ensure that the sampling rod sleeve can smoothly leave after being subjected to upward force, and the maximum size of the second limiting mechanism needs to ensure that the sampling rod sleeve still cannot leave the second limiting mechanism after being subjected to upward force.

[0016] Furthermore, the first limiting mechanism and the second limiting mechanism are respectively the first inverted cone and the second inverted cone; an elastic sheet is provided at the lower end of the sampling rod sleeve. In the initial state, the elastic sheet is expanded and combined with the first inverted cone. After the sampling is completed, the elastic sheet leaves the first inverted cone and slides into the groove and is combined with the second inverted cone. The elastic sheet retracts inward and can no longer be combined with the first inverted cone, and the sampling rod is extended.

[0017] In order to ensure that the elastic sheet at the lower end of the sampling rod is evenly stressed, the first limiting mechanism and the second limiting mechanism are preferably in the shape of an inverted frustum, thereby making the process of the sampling rod moving from the first limiting mechanism to the second limiting mechanism smoother.

[0018] In some embodiments, the cross-section of the upper end of the second rounded cone is larger than the cross-section of the upper end of the first rounded cone, so that the sampling rod sleeve is difficult to continue to stretch upward after moving to the second limiting mechanism.

[0019] It can be understood that the size of the cross-section of the upper end of the first inverted cone needs to be designed to just enable the elastic sheet at the lower end of the sampling rod sleeve to overcome a small amount of resistance and pass through when stretched upwards. In other words, the size of the cross-section of the upper end of the first inverted cone needs to be within a safe range where the elastic sheet can expand and not be damaged; and the size of the cross-section of the upper end of the second inverted cone is such that the sampling rod sleeve cannot be pulled out even if it is stretched upwards with force.

[0020] In some embodiments, the sampling rod sleeve is a hollow cylindrical structure, and the cross-section of the cylinder gradually shrinks downward; the elastic sheet at the lower end of the sampling rod sleeve can be directly cut on a circle of the cylindrical wall at the lower end of the sampling rod sleeve, and the number of elastic sheets can be two or more, which are symmetrically arranged.

[0021] When the sampling rod sleeve is inserted into the sampling rod from the lower end of the sampling rod, the sampling rod sleeve first contacts the first inverted cone on the sampling rod. Since the size of the upper part of the first inverted cone is larger than the original size of the elastic sheet on the sampling rod, the elastic sheet is expanded and closely attached to the outer wall of the first inverted cone. The sampling rod in this state is installed in the sample detection device and is in the initial state. The initial state of the present invention refers to that the sample detection device has not been used yet; the working state refers to that the sampling rod has completed sampling and is inserted for detection.

[0022] When sampling is required, during the process of pulling out the sampling rod from the sample detection device, the sampling rod sleeve moves upward from the first inverted cone position, and the elastic sheet is first expanded at the first limiting mechanism, and then enters the suddenly shrinking groove after crossing the first limiting mechanism, so that the elastic sheet immediately shrinks and fits the groove. At this time, the platform formed by the suddenly shrinking groove has a blocking effect on the elastic sheet, and even if it is compressed, it is difficult to make the elastic sheet return to the first limiting mechanism.

[0023] Furthermore, the sample collection component also includes a cup cover and a cup shoulder, and the cup shoulder is mounted on the cup body of the sample detection device and is combined with the cup body as an integral whole; the upper end of the cup shoulder can be detachably combined with the cup cover, and in the initial state, the cup cover and the cup shoulder are combined as an integral whole; the cup cover and the cup shoulder are separated when sampling.

[0024] In some embodiments, the inner side of the cup cover and the outer side of the upper end of the cup shoulder are provided with mutually matching threads, so that the cup cover and the cup shoulder are combined together through the threads.

[0025] Furthermore, the cup shoulder is provided with a hollow through hole, the sampling rod and the sampling rod sleeve are inserted into the cup body through the through hole, and the top end of the sampling rod sleeve is fixed to the cup cover as a whole.

[0026] The top of the sampling rod sleeve and the cup cover can be an integrally formed structure or a bonded structure. When the cup cover is opened, the sampling rod sleeve and the sampling rod pass through the cup shoulder and are pulled out, while the cup shoulder remains above the cup body and will not be pulled out because it is fixed as an integral structure with the cup body.

[0027] Furthermore, a resistance block is provided in the cup shoulder or on the sampling rod. In the initial state, the sampling rod and the cup shoulder are positioned and fixed by the resistance block, thereby providing resistance for the sampling rod to separate from the cup shoulder.

[0028] The existence of the resistance block makes it difficult to pull out the sampling rod and the sampling rod cover. The resistance brought by the resistance block must be overcome. At this time, compared with overcoming the resistance brought by the resistance block, the first limiting mechanism on the sampling rod brings less resistance to the sampling rod cover. Therefore, the sampling rod cover will first overcome the resistance of the first limiting mechanism and move to the second limiting mechanism, causing the sampling rod to be stretched. Then the sampling rod will overcome the resistance of the resistance block until it is completely pulled out.

[0029] Furthermore, an upper cavity and a lower cavity are provided in the cup body, and the upper cavity is provided with a connecting hole connected to the lower cavity. In the initial state, the piston is stuck on the connecting hole through a sealing ring, and the sample processing liquid is stored in the upper cavity sealed by the piston.

[0030] Furthermore, the lower cavity is composed of a base and the lower side of the cup body; the sample detection component includes a reagent strip and a card slot, the reagent strip is installed on the card slot and is connected to the lower cavity, when the piston is pushed open and falls by the sampling rod, the sample processing liquid containing the sample to be tested flows into the lower cavity and contacts the reagent strip.

[0031] This means that in the initial state, the piston divides the sample detection device into two cavities, the upper cavity is equipped with a sampling rod, and the sampling end of the sampling rod is immersed in the sample processing liquid; the lower cavity is connected to the sample end of the test reagent strip, and the lower cavity is basically empty in the initial state. In the working state, the extended sampling rod is inserted and the piston is pushed open and dropped, and the sample processing liquid containing the sample to be tested flows into the lower cavity and contacts the sample end of the reagent strip, thereby starting the test.

[0032] The sealing ring on the piston can ensure that the sample processing liquid is sealed in the upper cavity and does not leak into the lower cavity in the initial state.

[0033] In some methods, the volume of the sample processing liquid and the height of the sample end of the reagent strip can be controlled to precisely control the amount of sample processing liquid that the test strip contacts, thereby avoiding the problem of false negatives and false positives caused by low-concentration and high-concentration samples, making the test results more reliable.

[0034] In another aspect, the present invention provides a method for detecting a sample, the method comprising the following steps:

[0035] (1) The cup cover of the sample detection device is separated upward from the cup shoulder. During the separation process, the sampling rod sleeve slides upward from the first limiting mechanism of the sampling rod to the second limiting mechanism, and then the resistance block of the sampling rod slides into the cup shoulder;

[0036] (2) Sampling is completed using a sampling stick;

[0037] (3) The sampling rod with the cup cover is installed back into the sample detection device. The cup cover is assembled with the cup shoulder. During the assembly process, the resistance block of the sampling rod goes downward under the cup shoulder. The sampling rod pushes the piston open, and the sample liquid to be tested flows into the groove at the bottom of the cup body and contacts the reagent strip for sample detection.

[0038] In some embodiments, in step (1), the cup cover is separated from the cup shoulder by unscrewing the cup cover upwards. During the upward rotation of the cup cover, the sampling rod sleeve gradually slides upwards from the first limiting mechanism of the sampling rod to the second limiting mechanism, and then the resistance block of the sampling rod slides into the cup shoulder, and the sampling rod is successfully taken out.

[0039] The present invention has the following beneficial effects:

[0040] 1. By improving the structure of the sampling rod and the sampling rod sleeve, a first limiting mechanism and a second limiting mechanism are set on the sampling rod, so that the sampling rod sleeve can move upward on the sampling rod to extend the sampling rod as a whole. The length is sufficient to push open the lower piston, so that the sample liquid can be controlled to enter the lower cavity to contact the reagent strip and complete the detection;

[0041] 2. Both the first limiting mechanism and the second limiting mechanism are designed to be inverted truncated cone shapes, so that the elastic sheet at the lower end of the sampling rod can be evenly stressed, and the process of the sampling rod moving from the first limiting mechanism to the second limiting mechanism is smoother;

[0042] 3. By designing the resistance block, the first limit mechanism and the second limit mechanism to cooperate ingeniously, the sampling rod is extended during the extraction process and will not be shortened, thereby successfully completing the test;

[0043] 4. Realize automatic control of the liquid in the device when the device is fully closed;

[0044] 5. The device has a simple structure, is easy to use, and has low cost. It can ensure the accuracy and precision of the test, quickly provide more reliable test results, and can be tested anytime and anywhere. There are no professional requirements for users. Various people can use the sample detection device provided by the present invention to obtain effective test results more conveniently according to different needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 is a schematic diagram of the appearance structure of the sample detection device;

[0046] Figure 2 is a cross-sectional view of a sample detection device;

[0047] Figure 3 An exploded view of the sample detection device;

[0048] Figure 4 This is the schematic diagram of the extended sampling rod;

[0049] Figure 5 is a physical picture of the sample detection device, wherein the upper left picture is a front view, the upper right picture is a side view, and the lower picture is a top view;

[0050] Figure 6 This is the schematic diagram of pulling out, stretching and pushing open the piston of the sampling rod. DETAILED DESCRIPTION

[0051] The preferred embodiments of the present invention are further described in detail below in conjunction with the accompanying drawings. It should be pointed out that the following embodiments are intended to facilitate the understanding of the present invention and do not have any limiting effect on it. All features disclosed in the embodiments of the present invention, or all steps in the methods or processes disclosed, except for mutually exclusive features and / or steps, can be combined in any way.

[0052] Example 1: Sample detection device

[0053] The structure of the sample detection device 1 provided in this embodiment is as follows Figure 1 to Figure 6 As shown. Figure 1 and Figure 2 As shown, the sample detection device 1 provided in this embodiment includes a sample collection component 1, a sample processing component 2 and a sample detection component 3; the sample processing component 3 is filled with a sample processing liquid 4 and a piston 5, and the piston 5 is used to isolate the sample processing liquid 4 and the sample detection component 3; the sample collection component 1 includes a sampling rod 6, and the sampling rod 6 can extend and push the piston 5 after the sampling is completed, so that the sample liquid to be tested (including a mixed liquid of the sample processing liquid and the sample to be tested) is in contact with the sample detection component 3; the sample detection component 3 is used to detect the sample liquid to be tested.

[0054] like Figures 2 to 5 The sampling rod 6 is also covered with a sampling rod sleeve 7, and the sampling rod 6 is provided with a first limiting mechanism 8 and a second limiting mechanism 9 from bottom to top. In the initial state, the lower end of the sampling rod sleeve 7 is combined with the first limiting mechanism 8. After the sampling rod 6 completes sampling, the lower end of the sampling rod sleeve 7 is separated from the first limiting mechanism 8 and combined with the second limiting mechanism 9, thereby extending the sampling rod 6 and pushing the piston 5 open.

[0055] Preferably, both the first limiting mechanism 8 and the second limiting mechanism 9 have a section of structure that gradually thickens from bottom to top, and a groove 10 where the cross section of the sampling rod suddenly decreases from large to small exists at the connection between the first limiting mechanism 8 and the second limiting mechanism 9; the maximum size of the second limiting mechanism 9 (that is, the portion with the largest cross section) is larger than the maximum size of the first limiting mechanism 8. The suddenly reduced groove 10 forms a platform structure 11 at the connection between the first limiting mechanism 8 and the second limiting mechanism 9, and the platform structure 11 can prevent the sampling rod sleeve 7 from returning to the first limiting mechanism 8. The maximum size of the first limiting mechanism 8 needs to ensure that the sampling rod sleeve 7 can leave smoothly after being subjected to an upward force, and the maximum size of the second limiting mechanism 9 needs to ensure that the sampling rod sleeve 7 cannot leave the second limiting mechanism 9 after being subjected to an upward force.

[0056] The first limiting mechanism 8 and the second limiting mechanism 9 are respectively the first rounded platform 12 and the second rounded platform 13; the lower end of the sampling rod sleeve 7 is provided with an elastic sheet 14. Figure 4 As shown, in the initial state ( Figure 4 The elastic sheet 14 is expanded and combined with the first rounded platform 12, and the sampling is completed ( Figure 4 The elastic sheet 14 leaves the first inverted truncated platform 12, slides into the groove 10, and combines with the second inverted truncated platform 13. The elastic sheet 14 is retracted inward and can no longer be combined with the first inverted truncated platform 12, and the sampling rod 6 is extended. The inverted truncated platform-shaped structural design can make the elastic sheet 14 at the lower end of the sampling rod 6 evenly stressed, so that the process of moving the sampling rod 6 from the first limiting mechanism 8 to the second limiting mechanism 9 is smoother. The upper end cross section 15 of the second inverted truncated platform 13 is larger than the upper end cross section 16 of the first inverted truncated platform 12, so it is difficult for the sampling rod sleeve 7 to continue to stretch upward after moving to the second inverted truncated platform 13. At the same time, the size of the upper end cross section 16 of the first beveled platform 12 needs to be designed to just allow the elastic sheet 14 at the lower end of the sampling rod sleeve 7 to overcome a small resistance and pass through when stretched upward. In other words, the size of the upper end cross section 16 of the first beveled platform 12 needs to be within a safe range where the elastic sheet 14 expands and is not damaged; and the size of the upper end cross section 15 of the second beveled platform 13 is such that the sampling rod sleeve 7 cannot be pulled out even if it is stretched upward with force.

[0057] like Figure 3, the sampling rod sleeve 7 is a hollow cylindrical structure, and the cross-section of the cylinder gradually shrinks downward; the elastic sheet 14 at the lower end of the sampling rod sleeve 7 can be directly cut on a circle of cylindrical wall at the lower end of the sampling rod sleeve 7, and the number of elastic sheets 14 can be two or more, symmetrically arranged. When the sampling rod sleeve 7 starts to be inserted into the sampling rod 6 from the lower end of the sampling rod 6, the sampling rod sleeve 7 first contacts the first inverted platform 12 on the sampling rod 6. Since the size above the first inverted platform 12 is larger than the original size of the elastic sheet 14 on the sampling rod 6, the elastic sheet 14 is enlarged and tightly attached to the outer wall of the first inverted platform 12. In this state, the sampling rod 6 is installed in the sample detection device 1 and is in the initial state. The initial state means that the sample detection device 1 has not been used yet; the working state means that the sampling rod 6 has completed sampling and is inserted for detection. When sampling is required, during the process of pulling out the sampling rod 6 from the sample detection device 1, the sampling rod sleeve 7 moves upward from the first inverted table 12, and the elastic sheet 14 is first expanded at the first limiting mechanism 8, and then enters the suddenly shrinking groove 10 after crossing the first limiting mechanism 8, so that the elastic sheet 14 immediately shrinks and fits the groove 10. At this time, the platform structure 11 formed by the suddenly shrinking groove 10 has a blocking effect on the elastic sheet 14, and even if it is compressed, it is difficult to make the elastic sheet 14 return to the first limiting mechanism 8.

[0058] like Figure 2 and Figure 3 The sample collection component 1 also includes a cup cover 17 and a cup shoulder 18. The cup shoulder 18 is placed on the cup body 19 of the sample detection device 1 and is integrated with the cup body 19. The upper end of the cup shoulder 18 can be detachably assembled with the cup cover 17. In the initial state, the cup cover 17 and the cup shoulder 18 are integrated. When sampling, the cup cover 17 and the cup shoulder 18 are separated. In this embodiment, the inner side of the cup cover 17 and the outer side of the upper end of the cup shoulder 18 are provided with mutually matching threads, so that the cup cover 17 and the cup shoulder 18 are assembled together through the threads.

[0059] like Figure 3 The cup shoulder 18 is provided with a hollow through hole 20, through which the sampling rod 6 and the sampling rod sleeve 7 are inserted into the cup body 19, and the top of the sampling rod sleeve 7 is fixed as a whole with the cup cover 17. The top of the sampling rod sleeve 7 and the cup cover 17 can be an integrally formed structure or a structure bonded together. When the cup cover 17 is opened, the sampling rod sleeve 7 and the sampling rod 6 pass through the cup shoulder 18 and are pulled out, while the cup shoulder 18 is fixed as an integral structure with the cup body 19 and remains above the cup body 19 and will not be pulled out.

[0060] A resistance block 21 is provided in the cup shoulder 18 or on the sampling rod 6. The resistance block 21 and the first limiting mechanism 8 and the second limiting mechanism 9 act together to stretch and pull out the sampling rod 6. The process of pushing the piston 5 after sampling is as shown in FIG. Figure 6 As shown: In the initial state ( Figure 6The left figure, the cup body is omitted), the sampling rod 6 and the cup shoulder 18 are positioned and fixed by the resistance block 21, which provides resistance for the sampling rod 6 to be separated from the cup shoulder 18. The existence of the resistance block 21 makes it difficult to pull out the sampling rod 6 and the sampling rod sleeve 7. The resistance brought by the resistance block 21 must be overcome. At this time, compared with overcoming the resistance brought by the resistance block 21, the first limiting mechanism 8 on the sampling rod 6 brings less resistance to the sampling rod sleeve 7. Therefore, the sampling rod sleeve 7 will first overcome the resistance of the first limiting mechanism 8 and move to the second limiting mechanism 9, causing the sampling rod 6 to be stretched, and then the sampling rod 6 will overcome the resistance of the resistance block 21 until it is completely pulled out ( Figure 6 In the middle picture, the cup body is omitted). Figure 6 As shown in the right figure, after the extended sampling rod 6 is inserted, the piston 5 is pushed away and dropped, and the sample processing liquid containing the sample to be tested (the sample liquid to be tested) flows into the lower cavity 22 and contacts the sample end of the reagent strip, thereby starting the test.

[0061] like Figure 2 The cup body 19 is provided with an upper cavity 25 and a lower cavity 22. The upper cavity 25 is provided with a connecting hole 26 and communicated with the lower cavity 22. In the initial state, the piston 5 is stuck on the connecting hole 26 through the sealing ring 27, and the sample processing liquid 4 is stored in the upper cavity 25 sealed by the piston 5. The lower cavity 22 is composed of a base 28 and the lower side of the cup body 19; the sample detection component 3 includes a reagent strip and a card slot 29. The reagent strip is installed in the card slot 29 and communicated with the lower cavity 22. When the piston 5 is pushed away and dropped by the sampling rod 6, the sample processing liquid containing the sample to be tested flows into the lower cavity 22 and contacts the reagent strip.

[0062] It is equivalent to that in the initial state, the piston 5 divides the sample detection device 1 into two upper and lower cavities, the upper cavity 25 is equipped with a sampling rod 6, and the sampling end of the sampling rod 6 is immersed in the sample processing liquid; the lower cavity 22 is connected to the sample end of the detection reagent strip, and the lower cavity 22 is basically empty in the initial state. In the working state, the extended sampling rod 6 is inserted and the piston 5 is pushed away and dropped, and the sample processing liquid containing the sample to be tested flows into the lower cavity 22 and contacts the sample end of the reagent strip, thereby starting the detection. The sealing ring 27 on the piston 5 can achieve that in the initial state, the sample processing liquid is sealed in the upper cavity 25 and will not leak into the lower cavity 22. By controlling the volume of the sample processing liquid 4 and the height of the sample end of the reagent strip, the amount of the test strip 23 contacting the sample processing liquid 4 is accurately controlled, avoiding the problem of false negatives and false positives caused by low-concentration and high-concentration samples, making the detection results more reliable.

[0063] The detection method of the sample detection device 1 provided in this embodiment includes the following steps:

[0064] (1) The cup cover 17 of the sample detection device 1 is separated upward from the cup shoulder 18. During the separation process, the sampling rod sleeve 7 slides upward from the first limiting mechanism 8 of the sampling rod 6 to the second limiting mechanism 9, and then the resistance block 21 of the sampling rod 6 slides into the cup shoulder 18;

[0065] (2) Sampling is completed using the sampling rod 6;

[0066] (3) The sampling rod 6 with the cup cover 17 is installed back into the sample detection device 1, and the cup cover 17 is assembled with the cup shoulder 18. During the assembly process, the resistance block 21 of the sampling rod 6 moves downward under the cup shoulder 18, and the sampling rod 6 pushes the piston 5. The sample liquid to be tested flows into the lower cavity 22 at the bottom of the cup body 19 and contacts the reagent strip for sample detection.

[0067] Preferably, the cup cover 17 is separated from the cup shoulder 18 by unscrewing the cup cover 17 upwards. During the upward rotation of the cup cover 17, the sampling rod sleeve 7 gradually slides upwards from the first limiting mechanism 8 of the sampling rod 6 to the second limiting mechanism 9, and then the resistance block 21 of the sampling rod 6 slides into the cup shoulder 18, and the sampling rod 6 is smoothly taken out.

[0068] The application of the present invention is not limited thereto. For example, the scope of application of the present invention in reagent detection can be expanded. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention shall be based on the scope defined by the claims.

Claims

1. A sample detection device, characterized in that: It includes a sample collection component, a sample processing component and a sample detection component; the sample processing component is filled with a sample processing liquid and a piston, and the piston is used to isolate the sample processing liquid and the sample detection component; The sample collection component includes a sampling rod, which can be extended and push the piston after sampling is completed, so that the sample liquid to be tested contacts the sample detection component; The sample detection component is used to detect the sample liquid to be tested.

2. The sample detection device according to claim 1, characterized in that: The sampling rod is also covered with a sampling rod cover, and the sampling rod is provided with a first limiting mechanism and a second limiting mechanism from bottom to top. In the initial state, the lower end of the sampling rod cover is combined with the first limiting mechanism. After the sampling rod completes sampling, the lower end of the sampling rod cover is separated from the first limiting mechanism and combined with the second limiting mechanism, thereby extending the sampling rod and pushing the piston.

3. The sample detection device according to claim 2, characterized in that: The first limiting mechanism and the second limiting mechanism both have a section of structure that gradually thickens from bottom to top, and there is a groove at the connection between the first limiting mechanism and the second limiting mechanism, where the cross section of the sampling rod suddenly decreases from large to small; the maximum size of the second limiting mechanism is larger than the maximum size of the first limiting mechanism.

4. The sample detection device according to claim 3, characterized in that: The first limiting mechanism and the second limiting mechanism are respectively the first inverted cone and the second inverted cone, the upper end cross-section of the second inverted cone is larger than the upper end cross-section of the first inverted cone; an elastic sheet is provided at the lower end of the sampling rod sleeve, and in the initial state, the elastic sheet is expanded and combined with the first inverted cone. After the sampling is completed, the elastic sheet leaves the first inverted cone, slides into the groove, and is combined with the second inverted cone. The elastic sheet retracts inward and can no longer be combined with the first inverted cone, and the sampling rod is extended.

5. The sample detection device according to claim 4, characterized in that: The sample collection component also includes a cup cover and a cup shoulder, wherein the cup shoulder is mounted on the cup body of the sample detection device and is integrated with the cup body; the upper end of the cup shoulder can be detachably combined with the cup cover, and in the initial state, the cup cover and the cup shoulder are integrated; the cup cover and the cup shoulder are separated when sampling.

6. The sample detection device according to claim 5, characterized in that: The cup shoulder is provided with a hollow through hole, the sampling rod and the sampling rod sleeve are inserted into the cup body through the through hole, and the top end of the sampling rod sleeve is fixed to the cup cover as a whole.

7. The sample detection device according to claim 6, characterized in that: A resistance block is arranged in the cup shoulder or on the sampling rod. In the initial state, the sampling rod and the cup shoulder are positioned and fixed by the resistance block, so as to provide resistance for the sampling rod to separate from the cup shoulder.

8. The sample detection device according to claim 7, characterized in that: The cup body is provided with an upper cavity and a lower cavity, the upper cavity is provided with a connecting hole connected with the lower cavity, in the initial state, the piston is stuck on the connecting hole through a sealing ring, and the sample processing liquid is stored in the upper cavity sealed by the piston.

9. The sample detection device according to claim 8, characterized in that: The lower cavity is composed of a base and the lower side of the cup body; the sample detection component includes a reagent strip and a card slot, the reagent strip is installed on the card slot and is connected to the lower cavity, when the piston is pushed open and falls by the sampling rod, the sample processing liquid containing the sample to be tested flows into the lower cavity and contacts the reagent strip.

10. A method for detecting a sample, characterized in that: The following steps are involved: (1) The cup cover of the sample detection device is separated upward from the cup shoulder. During the separation process, the sampling rod sleeve slides upward from the first limiting mechanism of the sampling rod to the second limiting mechanism, and then the resistance block of the sampling rod slides into the cup shoulder; (2) Sampling is completed using a sampling stick; (3) The sampling rod with the cup cover is installed back into the sample detection device. The cup cover is assembled with the cup shoulder. During the assembly process, the resistance block of the sampling rod enters under the cup shoulder, the sampling rod pushes the piston, and the sample liquid to be tested flows into the groove at the bottom of the cup body and contacts the reagent strip for sample detection.

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