Environment-friendly biological plasticizer for biological specimen preservation and preparation method of environment-friendly biological plasticizer

Abstract

The invention relates to the technical field of plasticizers, in particular to an environment-friendly biological plasticizer for biological specimen preservation and a preparation method of the environment-friendly biological plasticizer. The environment-friendly biological plasticizer for preserving the biological specimen is prepared from the following raw materials in parts by weight: 10 to 20 parts of natural resin, 10 to 20 parts of carnauba wax, 20 to 30 parts of vegetable oil and 2 to 5 parts of functional additive, the environment-friendly biological plasticizer for preserving the biological specimens, prepared by the invention, not only has a better effect in the aspect of keeping the cellular morphology of the biological specimens and can effectively reduce the deformation and atrophy of the cells, but also has excellent environment-friendly performance and effectively ensures the quality of the biological specimens.

Description

Technical Field

[0001] The invention relates to the technical field of plasticizers, in particular to an environmentally friendly bioplasticizer for preserving biological specimens and a preparation method thereof. Background Art

[0002] In many fields such as biological science research and natural history museum display, the long-term and effective preservation of biological specimens is crucial. Traditional biological specimen preservation methods mainly rely on chemical preservatives and plasticizers.

[0003] Chemical preservatives such as formaldehyde, although they have certain antiseptic and bactericidal abilities and can prevent the corruption and degradation of biological tissues, formaldehyde has a strong irritating odor and is extremely harmful to human health. It is difficult to degrade in the environment and will have a negative impact on the ecological balance. Traditional plasticizers such as phthalates are widely used in the plasticization of biological specimens. However, phthalates are persistent and bioaccumulative in the natural environment, which will have an impact on the ecological environment.

[0004] Based on this, the present invention provides an environmentally friendly bioplasticizer for preserving biological specimens and a preparation method thereof to solve the above-mentioned technical problems. Summary of the invention

[0005] The purpose of the present invention is to provide an environmentally friendly bioplasticizer for preserving biological specimens and a preparation method thereof. The prepared environmentally friendly bioplasticizer for preserving biological specimens not only has a good effect in maintaining the cell morphology of biological specimens and can effectively reduce cell deformation and atrophy, but also has excellent environmental protection performance, effectively ensuring its quality.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] The first aspect of the present invention provides an environmentally friendly bioplasticizer for preserving biological specimens, which is composed of the following raw materials in parts by weight: 10 to 20 parts of natural resin, 10 to 20 parts of carnauba wax, 20 to 30 parts of vegetable oil and 2 to 5 parts of functional additives.

[0008] The present invention is further configured as follows: the natural resin is selected from any one of rosin and benzoin resin.

[0009] The present invention is further configured as follows: the vegetable oil is selected from any one of linseed oil and sunflower seed oil.

[0010] The present invention is further configured as follows: the functional auxiliary agent is formed by mixing rosemary extract and lavender extract in a mass ratio of 1:0.2-0.4.

[0011] The present invention is further configured as follows: the preparation process of the rosemary extract is as follows:

[0012] After the rosemary leaves are naturally air-dried, they are crushed into 100-200 meshes to obtain rosemary leaf powder;

[0013] The rosemary leaf powder is ultrasonically treated in a solvent at a solid-liquid ratio of 0.02 to 0.05 g / mL;

[0014] After the ultrasonic treatment is completed, the mixture is filtered, and the obtained filtrate is concentrated and dried to obtain a rosemary extract;

[0015] Wherein, the solvent is any one of ethanol or water;

[0016] The drying is freeze-drying at -20°C for 5 to 6 hours.

[0017] The present invention is further configured as follows: the ultrasonic power of the ultrasonic treatment is 360-380W, the ultrasonic temperature is 30-35°C, and the ultrasonic time is 30-33min.

[0018] The present invention is further configured as follows: the preparation process of the lavender extract is as follows:

[0019] The lavender petals are placed in an enzyme dispersion solution and treated at 45 to 55° C. for 45 to 60 minutes, and then the obtained enzymatic hydrolyzate is placed in a microwave extraction tank;

[0020] Adding 1.5-2.5% of the volume of the enzymatic hydrolysate to the microwave extraction tank, and microwave treatment at 65-70° C. for 100-120 min;

[0021] After suction filtration, the obtained filtrate is concentrated and dried to obtain lavender extract;

[0022] The drying step is performed at 50-60° C. to a constant weight.

[0023] The present invention is further configured as follows: the enzyme dispersion is obtained by mixing and activating the enzyme with water, the mass ratio of the enzyme to water is 0.03-0.06:1, and the enzyme is a mixture of cellulase, pectinase and glucanase in a mass ratio of 1:0.22-0.32:0.08-0.12.

[0024] The present invention is further configured as follows: the power of the microwave is 300-320W.

[0025] The second aspect of the present invention also provides a method for preparing the above-mentioned environmentally friendly bioplasticizer for preserving biological specimens, comprising the following steps:

[0026] Step 1: Accurately weigh natural resin, carnauba wax, vegetable oil and functional additives and set aside;

[0027] Step 2: put the natural resin, carnauba wax and vegetable oil into a mixing device, and stir and mix at a temperature of 70 to 80° C. for 20 to 30 minutes;

[0028] Step 3: Cool down to 40-50° C., add functional additives thereto, and continue stirring and mixing for 15-20 minutes to obtain an environmentally friendly bioplasticizer product for preserving biological specimens.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] In the present invention, natural resin, carnauba wax, vegetable oil and functional additives are used as raw materials. The natural resin, carnauba wax and vegetable oil are put into a mixing device and stirred and mixed, and then the functional additives are added after cooling, and the stirring and mixing are continued to obtain an environmentally friendly bioplasticizer product for preserving biological specimens. The environmentally friendly bioplasticizer prepared by the present invention for preserving biological specimens not only has a good effect in maintaining the cell morphology of biological specimens, but can also effectively reduce the deformation and atrophy of cells, and also has excellent environmental protection performance, effectively ensuring its quality and quality. The environmentally friendly bioplasticizer for preserving biological specimens provided by the present invention and the preparation method thereof have a broader market prospect and are more suitable for promotion. DETAILED DESCRIPTION

[0031] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Example 1

[0033] The present embodiment provides an environmentally friendly bioplasticizer for preserving biological specimens, which is composed of the following raw materials in parts by weight: 10 parts of natural resin, 10 parts of carnauba wax, 20 parts of vegetable oil and 2 parts of functional additives.

[0034] Among them, rosin is selected as the natural resin.

[0035] In this embodiment, it should be noted that rosin was purchased from Shandong Huayu Chemical Technology Co., Ltd.

[0036] The vegetable oil of choice is linseed oil.

[0037] The functional auxiliary agent is prepared by mixing rosemary extract and lavender extract in a mass ratio of 1:0.2.

[0038] Further, the preparation process of rosemary extract is as follows:

[0039] After the rosemary leaves are naturally air-dried, they are crushed into 100 meshes to obtain rosemary leaf powder;

[0040] The rosemary leaf powder was ultrasonically treated in a solvent at a solid-liquid ratio of 0.02 g / mL;

[0041] After the ultrasonic treatment is completed, the mixture is filtered, and the obtained filtrate is concentrated and dried to obtain a rosemary extract;

[0042] Wherein, the solvent is ethanol;

[0043] The drying is freeze-drying at -20°C for 5 hours.

[0044] Furthermore, the ultrasonic power of the ultrasonic treatment is 360 W, the ultrasonic temperature is 30° C., and the ultrasonic time is 30 min.

[0045] In addition, the preparation process of the lavender extract is as follows:

[0046] Lavender petals were placed in enzyme dispersion at 45°C for 45 minutes, and then the obtained enzymatic hydrolyzate was placed in a microwave extraction tank;

[0047] Add 1.5% of the volume of the enzymatic hydrolysate in anhydrous ethanol into the microwave extraction tank, and microwave the mixture at 65°C for 100 min.

[0048] After suction filtration, the obtained filtrate is concentrated and dried to obtain lavender extract;

[0049] The drying is carried out at 50° C. to a constant weight.

[0050] Furthermore, the enzyme dispersion is obtained by mixing and activating the enzyme with water, wherein the mass ratio of the enzyme to water is 0.03:1, and the enzyme is a mixture of cellulase, pectinase and glucanase in a mass ratio of 1:0.22:0.08.

[0051] The power of the microwave is 300W.

[0052] In addition, this embodiment also provides a method for preparing the above-mentioned environmentally friendly bioplasticizer for preserving biological specimens, comprising the following steps:

[0053] Step 1: Accurately weigh natural resin, carnauba wax, vegetable oil and functional additives and set aside;

[0054] Step 2: put the natural resin, carnauba wax and vegetable oil into a mixing device, and stir and mix at a temperature of 70° C. for 20 minutes;

[0055] Step 3: Cool down to 40° C., add functional additives thereto, and continue stirring and mixing for 15 minutes to obtain an environmentally friendly bioplasticizer product for preserving biological specimens.

[0056] Example 2

[0057] The preparation method of the environmentally friendly bioplasticizer for biological specimen preservation provided in this embodiment is basically the same as that in Example 1, except that: the specific raw material composition and the specific preparation method of the environmentally friendly bioplasticizer for biological specimen preservation in this embodiment are different; the specific raw material composition and the specific preparation method of the environmentally friendly bioplasticizer for biological specimen preservation in this embodiment are as follows:

[0058] An environmentally friendly bioplasticizer for preserving biological specimens is composed of the following raw materials in parts by weight: 12 parts of natural resin, 12 parts of carnauba wax, 22 parts of vegetable oil and 3 parts of functional additives.

[0059] Among them, the natural resin is benzoin resin.

[0060] In this embodiment, it should be noted that the benzoin resin was purchased from Jiangsu Caiwei Biotechnology Co., Ltd.

[0061] The vegetable oil used is sunflower seed oil.

[0062] The functional additive is prepared by mixing rosemary extract and lavender extract in a mass ratio of 1:0.3.

[0063] Further, the preparation process of rosemary extract is as follows:

[0064] After the rosemary leaves are naturally air-dried, they are crushed into 150 meshes to obtain rosemary leaf powder;

[0065] The rosemary leaf powder was ultrasonically treated in a solvent at a solid-liquid ratio of 0.03 g / mL;

[0066] After the ultrasonic treatment is completed, the mixture is filtered, and the obtained filtrate is concentrated and dried to obtain a rosemary extract;

[0067] Wherein, the solvent is water;

[0068] The drying is freeze-drying at -20°C for 5 hours.

[0069] Furthermore, the ultrasonic power of the ultrasonic treatment was 362 W, ​​the ultrasonic temperature was 31° C., and the ultrasonic time was 31 min.

[0070] In addition, the preparation process of the lavender extract is as follows:

[0071] Lavender petals were placed in enzyme dispersion at 47°C for 47 minutes, and then the obtained enzymatic hydrolyzate was placed in a microwave extraction tank;

[0072] Add 1.6% of the volume of the enzymatic hydrolysate in anhydrous ethanol into the microwave extraction tank, and microwave for 105 min at 66°C;

[0073] After suction filtration, the obtained filtrate is concentrated and dried to obtain lavender extract;

[0074] The drying step is performed at 52° C. to a constant weight.

[0075] Furthermore, the enzyme dispersion is obtained by mixing and activating the enzyme with water, wherein the mass ratio of the enzyme to water is 0.04:1, and the enzyme is a mixture of cellulase, pectinase and glucanase in a mass ratio of 1:0.24:0.09.

[0076] The power of the microwave is 305W.

[0077] In addition, this embodiment also provides a method for preparing the above-mentioned environmentally friendly bioplasticizer for preserving biological specimens, comprising the following steps:

[0078] Step 1: Accurately weigh natural resin, carnauba wax, vegetable oil and functional additives and set aside;

[0079] Step 2: put the natural resin, carnauba wax and vegetable oil into a mixing device, and stir and mix at a temperature of 72° C. for 22 minutes;

[0080] Step 3: Cool down to 42° C., add functional additives thereto, and continue stirring and mixing for 16 minutes to obtain an environmentally friendly bioplasticizer product for preserving biological specimens.

[0081] Example 3

[0082] The preparation method of the environmentally friendly bioplasticizer for biological specimen preservation provided in this embodiment is basically the same as that in Example 1, except that: the specific raw material composition and the specific preparation method of the environmentally friendly bioplasticizer for biological specimen preservation in this embodiment are different; the specific raw material composition and the specific preparation method of the environmentally friendly bioplasticizer for biological specimen preservation in this embodiment are as follows:

[0083] An environmentally friendly bioplasticizer for preserving biological specimens is composed of the following raw materials in parts by weight: 15 parts of natural resin, 15 parts of carnauba wax, 25 parts of vegetable oil and 4 parts of functional additives.

[0084] Among them, rosin is selected as the natural resin.

[0085] In this embodiment, it should be noted that rosin was purchased from Shandong Huayu Chemical Technology Co., Ltd.

[0086] The vegetable oil of choice is linseed oil.

[0087] The functional additive is prepared by mixing rosemary extract and lavender extract in a mass ratio of 1:0.3.

[0088] Further, the preparation process of rosemary extract is as follows:

[0089] After the rosemary leaves are naturally air-dried, they are crushed into 150 meshes to obtain rosemary leaf powder;

[0090] The rosemary leaf powder was ultrasonically treated in a solvent at a solid-liquid ratio of 0.04 g / mL;

[0091] After the ultrasonic treatment is completed, the mixture is filtered, and the obtained filtrate is concentrated and dried to obtain a rosemary extract;

[0092] Wherein, the solvent is ethanol;

[0093] The drying is freeze-drying at -20°C for 6 hours.

[0094] Furthermore, the ultrasonic power of the ultrasonic treatment was 370 W, the ultrasonic temperature was 33° C., and the ultrasonic time was 32 min.

[0095] In addition, the preparation process of the lavender extract is as follows:

[0096] Lavender petals were placed in enzyme dispersion at 50°C for 52 minutes, and then the resulting enzymatic hydrolyzate was placed in a microwave extraction tank;

[0097] Add 2% of the volume of the enzymatic hydrolysate in anhydrous ethanol into the microwave extraction tank, and microwave for 110 min at 67°C;

[0098] After suction filtration, the obtained filtrate is concentrated and dried to obtain lavender extract;

[0099] The drying is carried out at 55° C. to a constant weight.

[0100] Furthermore, the enzyme dispersion is obtained by mixing and activating the enzyme with water, wherein the mass ratio of the enzyme to water is 0.05:1, and the enzyme is a mixture of cellulase, pectinase and glucanase in a mass ratio of 1:0.27:0.1.

[0101] The power of the microwave is 310W.

[0102] In addition, this embodiment also provides a method for preparing the above-mentioned environmentally friendly bioplasticizer for preserving biological specimens, comprising the following steps:

[0103] Step 1: Accurately weigh natural resin, carnauba wax, vegetable oil and functional additives and set aside;

[0104] Step 2: put the natural resin, carnauba wax and vegetable oil into a mixing device, and stir and mix at a temperature of 75° C. for 25 minutes;

[0105] Step 3: Cool down to 45° C., add functional additives thereto, and continue stirring and mixing for 17 minutes to obtain an environmentally friendly bioplasticizer product for preserving biological specimens.

[0106] Example 4

[0107] The preparation method of the environmentally friendly bioplasticizer for biological specimen preservation provided in this embodiment is basically the same as that in Example 1, except that: the specific raw material composition and the specific preparation method of the environmentally friendly bioplasticizer for biological specimen preservation in this embodiment are different; the specific raw material composition and the specific preparation method of the environmentally friendly bioplasticizer for biological specimen preservation in this embodiment are as follows:

[0108] An environmentally friendly bioplasticizer for preserving biological specimens is composed of the following raw materials in parts by weight: 18 parts of natural resin, 18 parts of carnauba wax, 28 parts of vegetable oil and 4 parts of functional additives.

[0109] Among them, rosin is selected as the natural resin.

[0110] In this embodiment, it should be noted that rosin was purchased from Shandong Huayu Chemical Technology Co., Ltd.

[0111] The vegetable oil of choice is linseed oil.

[0112] The functional additive is prepared by mixing rosemary extract and lavender extract in a mass ratio of 1:0.3.

[0113] Further, the preparation process of rosemary extract is as follows:

[0114] After the rosemary leaves are naturally air-dried, they are crushed to 200 meshes to obtain rosemary leaf powder;

[0115] The rosemary leaf powder was ultrasonically treated in a solvent at a solid-liquid ratio of 0.05 g / mL;

[0116] After the ultrasonic treatment is completed, the mixture is filtered, and the obtained filtrate is concentrated and dried to obtain a rosemary extract;

[0117] Wherein, the solvent is ethanol;

[0118] The drying is freeze-drying at -20°C for 6 hours.

[0119] Furthermore, the ultrasonic power of the ultrasonic treatment was 375 W, the ultrasonic temperature was 34° C., and the ultrasonic time was 32 min.

[0120] In addition, the preparation process of the lavender extract is as follows:

[0121] Lavender petals were placed in enzyme dispersion at 52°C for 57 minutes, and then the resulting enzymatic hydrolyzate was placed in a microwave extraction tank;

[0122] Add 2.2% of the volume of the enzymatic hydrolysate in anhydrous ethanol into the microwave extraction tank, and microwave for 115 min at 68°C;

[0123] After suction filtration, the obtained filtrate is concentrated and dried to obtain lavender extract;

[0124] The drying step is performed at 58° C. to a constant weight.

[0125] Furthermore, the enzyme dispersion is obtained by mixing and activating the enzyme with water, wherein the mass ratio of the enzyme to water is 0.05:1, and the enzyme is a mixture of cellulase, pectinase and glucanase in a mass ratio of 1:0.3:0.11.

[0126] The power of the microwave is 315W.

[0127] In addition, this embodiment also provides a method for preparing the above-mentioned environmentally friendly bioplasticizer for preserving biological specimens, comprising the following steps:

[0128] Step 1: Accurately weigh natural resin, carnauba wax, vegetable oil and functional additives and set aside;

[0129] Step 2: put the natural resin, carnauba wax and vegetable oil into a mixing device, and stir and mix at a temperature of 78° C. for 28 minutes;

[0130] Step 3: Cool down to 40-50° C., add functional additives thereto, and continue stirring and mixing for 18 minutes to obtain an environmentally friendly bioplasticizer product for preserving biological specimens.

[0131] Example 5

[0132] The preparation method of the environmentally friendly bioplasticizer for biological specimen preservation provided in this embodiment is basically the same as that in Example 1, except that: the specific raw material composition and the specific preparation method of the environmentally friendly bioplasticizer for biological specimen preservation in this embodiment are different; the specific raw material composition and the specific preparation method of the environmentally friendly bioplasticizer for biological specimen preservation in this embodiment are as follows:

[0133] An environmentally friendly bioplasticizer for preserving biological specimens is composed of the following raw materials in parts by weight: 20 parts of natural resin, 20 parts of carnauba wax, 30 parts of vegetable oil and 5 parts of functional additives.

[0134] Among them, rosin is selected as the natural resin.

[0135] In this embodiment, it should be noted that rosin was purchased from Shandong Huayu Chemical Technology Co., Ltd.

[0136] The vegetable oil of choice is linseed oil.

[0137] The functional auxiliary agent is prepared by mixing rosemary extract and lavender extract in a mass ratio of 1:0.4.

[0138] Further, the preparation process of rosemary extract is as follows:

[0139] After the rosemary leaves are naturally air-dried, they are crushed to 200 meshes to obtain rosemary leaf powder;

[0140] The rosemary leaf powder was ultrasonically treated in a solvent at a solid-liquid ratio of 0.05 g / mL;

[0141] After the ultrasonic treatment is completed, the mixture is filtered, and the obtained filtrate is concentrated and dried to obtain a rosemary extract;

[0142] Wherein, the solvent is ethanol;

[0143] The drying is freeze-drying at -20°C for 6 hours.

[0144] Furthermore, the ultrasonic power of the ultrasonic treatment was 380 W, the ultrasonic temperature was 35° C., and the ultrasonic time was 33 min.

[0145] In addition, the preparation process of the lavender extract is as follows:

[0146] Lavender petals were placed in enzyme dispersion at 55°C for 60 minutes, and then the obtained enzymatic hydrolyzate was placed in a microwave extraction tank;

[0147] Add 2.5% of the volume of the enzymatic hydrolysate in anhydrous ethanol into the microwave extraction tank, and microwave for 120 min at 70°C;

[0148] After suction filtration, the obtained filtrate is concentrated and dried to obtain lavender extract;

[0149] The drying is carried out at 60° C. to a constant weight.

[0150] Furthermore, the enzyme dispersion is obtained by mixing and activating the enzyme with water, wherein the mass ratio of the enzyme to water is 0.06:1, and the enzyme is a mixture of cellulase, pectinase and glucanase in a mass ratio of 1:0.32:0.12.

[0151] The power of the microwave is 320W.

[0152] In addition, this embodiment also provides a method for preparing the above-mentioned environmentally friendly bioplasticizer for preserving biological specimens, comprising the following steps:

[0153] Step 1: Accurately weigh natural resin, carnauba wax, vegetable oil and functional additives and set aside;

[0154] Step 2: put the natural resin, carnauba wax and vegetable oil into a mixing device, and stir and mix at a temperature of 80° C. for 30 minutes;

[0155] Step 3: Cool down to 50° C., add functional additives thereto, and continue stirring and mixing for 20 minutes to obtain an environmentally friendly bioplasticizer product for preserving biological specimens.

[0156] Performance test: The samples of the environmentally friendly bioplasticizers provided in Examples 1 to 5 are respectively marked as Examples 1 to 5; and the relevant properties of the environmentally friendly bioplasticizers provided in Examples 1 to 5 are respectively tested as follows:

[0157] 1. Curing morphology test: The test method is to take the environmentally friendly bioplasticizer samples provided in Examples 1 to 5 as Example 1 to 5 groups, respectively, and take a commercially available bioplasticizer (Foshan Mingchenda Silicone Co., Ltd., Mingchenda Silicone) as a control group; the experimental material is mouse liver tissue; the specimens are processed according to conventional methods; after 6 weeks, a group of 10 experts respectively score the cell morphology changes and take the average value (0-10, 0 for no change, 10 for severe deformation).

[0158] 2. Environmental testing: The testing method is to use gas chromatography-mass spectrometry (GC-MS) to detect the release of volatile organic compounds (VOC) on samples treated with plasticizers.

[0159] The obtained test data are recorded in Table 1 and Table 2 below:

[0160] Table 1 Test results of curing morphology performance of each group

[0161]

[0162]

[0163] Table 2 Environmental performance test results of each group

[0164] Group <![CDATA[VOC emission rate (mg / m 3 )]]> Example 1 Group 1.2 Example 2 Group 1.1 Example 3 Group 1.2 Example 4 group 1.3 Example 5 group 1.2 Control group 5.2

[0165] By comparing and analyzing the relevant data in Table 1 and Table 2, it can be seen that the environmentally friendly bioplasticizer for biological specimen preservation prepared by the present invention not only has a good effect in maintaining the cell morphology of the biological specimen, can effectively reduce the deformation and atrophy of the cell, but also has excellent environmental performance, effectively ensuring its quality. This shows that the environmentally friendly bioplasticizer for biological specimen preservation and the preparation method thereof provided by the present invention have a broader market prospect and are more suitable for promotion.

[0166] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0167] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. An environmentally friendly bioplasticizer for preserving biological specimens, characterized in that: It is composed of the following raw materials in parts by weight: 10-20 parts of natural resin, 10-20 parts of carnauba wax, 20-30 parts of vegetable oil and 2-5 parts of functional additives.

2. The environmentally friendly bioplasticizer for preserving biological specimens according to claim 1, characterized in that: The natural resin is selected from any one of rosin and benzoin resin.

3. The environmentally friendly bioplasticizer for preserving biological specimens according to claim 1, characterized in that: The vegetable oil is selected from any one of linseed oil and sunflower seed oil.

4. The environmentally friendly bioplasticizer for preserving biological specimens according to claim 1, characterized in that: The functional auxiliary agent is prepared by mixing rosemary extract and lavender extract in a mass ratio of 1:0.2-0.

4.

5. The environmentally friendly bioplasticizer for preserving biological specimens according to claim 4, characterized in that: The preparation process of the rosemary extract is as follows: After the rosemary leaves are naturally air-dried, they are crushed into 100-200 meshes to obtain rosemary leaf powder; The rosemary leaf powder is ultrasonically treated in a solvent at a solid-liquid ratio of 0.02 to 0.05 g / mL; After the ultrasonic treatment is completed, the mixture is filtered, and the obtained filtrate is concentrated and dried to obtain a rosemary extract; Wherein, the solvent is any one of ethanol or water; The drying is freeze-drying at -20°C for 5 to 6 hours.

6. The environmentally friendly bioplasticizer for preserving biological specimens according to claim 5, characterized in that: The ultrasonic treatment has an ultrasonic power of 360-380 W, an ultrasonic temperature of 30-35° C., and an ultrasonic time of 30-33 min.

7. The environmentally friendly bioplasticizer for preserving biological specimens according to claim 4, characterized in that: The preparation process of the lavender extract is as follows: The lavender petals are placed in an enzyme dispersion solution and treated at 45 to 55° C. for 45 to 60 minutes, and then the obtained enzymatic hydrolyzate is placed in a microwave extraction tank; Adding 1.5-2.5% of the volume of the enzymatic hydrolysate to the microwave extraction tank, and microwave treatment at 65-70° C. for 100-120 min; After suction filtration, the obtained filtrate is concentrated and dried to obtain lavender extract; The drying step is performed at 50-60° C. to a constant weight.

8. The environmentally friendly bioplasticizer for preserving biological specimens according to claim 7, characterized in that: The enzyme dispersion is obtained by mixing and activating the enzyme with water, the mass ratio of the enzyme to water is 0.03-0.06:1, and the enzyme is prepared by mixing cellulase, pectinase and glucanase in a mass ratio of 1:0.22-0.32:0.08-0.

12.

9. The environmentally friendly bioplasticizer for preserving biological specimens according to claim 7, characterized in that: The power of the microwave is 300-320W.

10. A method for preparing an environmentally friendly bioplasticizer for preserving biological specimens according to any one of claims 1 to 9, characterized in that: The following steps are involved: Step 1: Accurately weigh natural resin, carnauba wax, vegetable oil and functional additives and set aside; Step 2: put the natural resin, carnauba wax and vegetable oil into a mixing device, and stir and mix at a temperature of 70 to 80° C. for 20 to 30 minutes; Step 3: Cool down to 40-50° C., add functional additives thereto, and continue stirring and mixing for 15-20 minutes to obtain an environmentally friendly bioplasticizer product for preserving biological specimens.

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