System and method for evaluating performance of low-temperature nitrifying bacteria agent

Abstract

The invention relates to the technical field of sewage treatment, and particularly discloses a low-temperature nitrifying bacterial agent performance evaluation system and method.The system comprises a water inlet unit, a bacterial agent activation module, an evaluation reactor, a low-temperature circulation unit, an online monitoring unit and an automatic control unit; the water inlet unit is respectively connected with the fungicide activation module and the evaluation reactor; the microbial inoculum activation module is used for accurately putting a low-temperature nitrifying microbial inoculum into the evaluation reactor; the evaluation reactor is used for evaluating the ammonia nitrogen removal capability of the microbial inoculum under a low-temperature condition; the low-temperature circulating unit is used for controlling the temperature of the evaluation reactor; and the online monitoring unit is used for monitoring and evaluating the reactor and sending a monitoring signal to the automatic control unit. The device has the advantages of being reasonable in structural design, reliable and stable in operation, high in automation and intelligence degree, wide in application range and the like, and can simulate an actual low-temperature environment and accurately and efficiently evaluate and evaluate the performance of the low-temperature nitrifying bacterium agent so as to screen out efficient nitrifying bacterium agents suitable for low-temperature application of different water qualities.

Description

Technical Field

[0001] The present invention relates to the technical field of sewage treatment, and in particular to a system and method for evaluating the performance of a low-temperature nitrifying bacteria agent. Background Art

[0003] Some nitrifying bacteria, such as Pseudomonas, Acinetobacter, and Bacillus, still have good ammonia nitrogen removal capabilities in low-temperature environments, but many bacterial agents on the market do not have low-temperature nitrification effects when actually used. At present, there is a lack of accurate, efficient evaluation systems and methods that can simulate actual low-temperature environments to evaluate the performance of low-temperature nitrifying agents, making it difficult to compare and screen the quality of low-temperature nitrifying agents of different brands or batches. Therefore, it is of great significance to provide a system and method for evaluating the performance of low-temperature nitrifying agents that is not affected by temperature, has a high degree of automation, and is user-friendly.

[0004] Based on this technical background, the present invention studies a low-temperature nitrifying bacteria agent performance evaluation system and method. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a low-temperature nitrifying bacteria agent performance evaluation system and method. The system has the advantages of reasonable structural design, reliable and stable operation, high degree of automation and intelligence, and wide range of applications. It can simulate the actual low-temperature environment, accurately and efficiently evaluate and assess the performance of low-temperature nitrifying bacteria agents, so as to screen out high-efficiency nitrifying bacteria agents suitable for low-temperature applications in different water qualities.

[0006] In order to achieve the above-mentioned object, the first aspect of the present invention provides a low-temperature nitrifying bacteria agent performance evaluation system, including a water inlet unit, a bacteria agent activation module, an evaluation reactor, a low-temperature circulation unit, an online monitoring unit and an automatic control unit;

[0007] The water inlet unit is connected to the bacterial agent activation module and the evaluation reactor respectively;

[0008] The bacterial agent activation module is used to evaluate the activation and recovery of the bacterial agent and accurately put the low-temperature nitrifying bacterial agent into the evaluation reactor;

[0009] The evaluation reactor is connected to the low-temperature circulation unit and is used to evaluate the ability of the bacterial agent to remove ammonia nitrogen under low-temperature conditions;

[0010] The low temperature circulation unit is used to control the temperature of the evaluation reactor;

[0011] The online monitoring unit is connected to the evaluation reactor and the automatic control unit respectively, and is used to monitor the evaluation reactor and send a monitoring signal to the automatic control unit.

[0012] The second aspect of the present invention provides a method for evaluating the performance of a low-temperature nitrifying bacteria agent in the above-mentioned device, comprising:

[0013] Bacterial agent activation: determine the bacterial growth curve and clarify the optimal conditions required for bacterial agent activation;

[0014] Bacterial agent component analysis: Analyze the COD content and bacterial component composition of the bacterial agent;

[0015] Bacterial agent nitrification performance test: The nitrification performance of the bacterial agent is tested through the operation process of preparation, activated sludge addition, substrate addition, bacterial agent addition and reaction start-up;

[0016] Reaction system activity analysis: analysis or determination of one or more of sludge concentration, sludge settling performance, biological phase, enzyme activity, adenosine triphosphate and specific oxygen consumption rate;

[0017] Result output and analysis: The system data is monitored in real time using an online monitoring unit, and the system data is transmitted to a data processing center, through which the ammonia nitrogen removal rate, ammonia nitrogen degradation rate, nitrification rate, and nitrite accumulation rate are automatically calculated for performance evaluation.

[0018] The beneficial effects of the present invention include:

[0019] (1) The low-temperature nitrifying bacteria performance evaluation system proposed in the present invention has the advantages of reasonable structural design, reliable and stable operation, high degree of automation and intelligence, and wide application range. It can simulate the actual low-temperature environment and accurately and efficiently evaluate and assess the performance of low-temperature nitrifying bacteria, so as to screen out high-efficiency nitrifying bacteria suitable for low-temperature application in different water qualities.

[0020] (2) The low-temperature nitrifying bacteria performance evaluation system proposed in the present invention can monitor the reaction parameters in real time and obtain the calculated indicators through the data processing system, so as to obtain data efficiently and reliably; at the same time, it can accurately simulate the low-temperature environment and accurately evaluate the performance of the low-temperature nitrifying bacteria; the scientific evaluation indicators can comprehensively reflect the activity and nitrification performance of the bacteria in the low-temperature environment, so as to ensure the stability and effectiveness of the bacteria in practical applications.

[0021] (3) The low-temperature nitrifying bacteria performance evaluation system proposed in the present invention is helpful to screen high-quality nitrifying bacteria in low-temperature environments, screen out low-temperature and high-efficiency nitrifying bacteria suitable for different water qualities, and improve the nitrogen removal efficiency in applications such as sewage treatment. It has a high degree of self-control and a strong operability of the evaluation method, so that ordinary personnel can quickly measure the nitrification performance of low-temperature nitrifying bacteria in batches.

[0022] Other features and advantages of the present invention will be described in detail in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The above and other objects, features and advantages of the present invention will become more apparent through a more detailed description of exemplary embodiments of the present invention with reference to the accompanying drawings.

[0024] Figure 1 This is a schematic diagram of the structure of the low-temperature nitrifying bacteria agent performance evaluation system proposed in the present invention.

[0025] Figure 2 The figure is a flow chart of a specific implementation of the low-temperature nitrifying bacteria agent performance evaluation system proposed in the present invention.

[0026] Figure 3 This is a schematic diagram of the bacterial composition analysis of the bacterial agent in a specific implementation of the low-temperature nitrifying bacterial agent performance evaluation system proposed in the present invention. DETAILED DESCRIPTION

[0027] The preferred embodiments of the present invention will be described in more detail below. Although the preferred embodiments of the present invention are described below, it should be understood that the present invention can be implemented in various forms and should not be limited to the embodiments set forth herein.

[0028] The present invention provides a low-temperature nitrifying bacteria agent performance evaluation system, such as Figure 1 As shown, it includes a water inlet unit, a bacterial agent activation module, an evaluation reactor, a low-temperature circulation unit, an online monitoring unit and an automatic control unit;

[0029] The water inlet unit is connected to the bacterial agent activation module and the evaluation reactor respectively;

[0030] The bacterial agent activation module is used to evaluate the activation and recovery of the bacterial agent and accurately put the low-temperature nitrifying bacterial agent into the evaluation reactor;

[0031] The evaluation reactor is connected to the low-temperature circulation unit to evaluate the ability of the bacterial agent to remove ammonia nitrogen under low-temperature conditions;

[0032] The low temperature circulation unit is used to control the temperature of the evaluation reactor;

[0033] The online monitoring unit is connected to the evaluation reactor and the automatic control unit respectively, and is used for monitoring the evaluation reactor and sending the monitoring signal to the automatic control unit.

[0034] The present invention has the advantages of reasonable structural design, reliable and stable operation, high degree of automation and intelligence, and wide application range. It can simulate the actual low-temperature environment, accurately and efficiently test and evaluate the performance of low-temperature nitrifying bacteria agents, so as to screen out high-efficiency nitrifying bacteria agents suitable for low-temperature application in different water qualities.

[0035] According to the present invention, the water inlet unit comprises a water inlet tank, a metering pump and a delivery pipeline;

[0036] The inlet water tank is used to store the sewage or water required for evaluation;

[0037] The metering pump is used to accurately deliver sewage or experimental water according to the set flow rate to ensure the stability of the substrate concentration during the reaction process;

[0038] The bacterial agent activation module is equipped with a heating module, a stirring device, an aeration module and a high-precision metering device to realize the revival and quantitative delivery of the bacterial agent, ensuring that the initial inoculation amount of the bacterial agent is accurate and consistent in each evaluation.

[0039] According to the present invention, the evaluation reactor is cylindrical, with a low-temperature circulation layer and a heat preservation layer arranged on the outside in sequence, a sampling port reserved on the top for the installation of online instruments, a stirring device and an aeration module arranged inside, and a plurality of water inlet and outlet valves arranged for water inlet and outlet and sampling respectively;

[0040] The low temperature circulation unit is connected to the low temperature circulation layer, and controls the temperature range of the reaction system to be -5°C to 90°C;

[0041] The low temperature circulation unit is a low temperature water bath or a semiconductor refrigeration sheet;

[0042] The aeration module is an aeration unit with a flow meter, which is used to control the aeration volume in real time. It can control the dissolved oxygen range from 0 to 8 mg / L.

[0043] The stirring device is a stirrer with controllable stirring speed, and the stirrer is a two-blade paddle stirrer, which is used to fully mix the sewage and the sludge.

[0044] In the present invention, the reaction parameters can be monitored in real time, and the calculated indicators can be obtained through the data processing system, so that the data can be obtained efficiently and reliably; at the same time, the low-temperature environment can be accurately simulated, and the performance of the low-temperature nitrifying bacteria agent can be accurately evaluated; the scientific evaluation indicators can comprehensively reflect the activity and nitrification performance of the bacteria agent under the low-temperature environment, so as to ensure the stability and effectiveness of the bacteria agent in practical applications.

[0045] According to the present invention, the online monitoring unit includes a temperature sensor, an online monitor for pH, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, sludge concentration meter, and dissolved oxygen, which are arranged at a preset position of the evaluation reactor;

[0046] Online monitoring signals of temperature sensor, pH, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, sludge concentration meter and dissolved oxygen are sent to the automatic control unit.

[0047] According to the present invention, the automatic control unit includes a programmable controller, a memory, a data processing center and a data display panel;

[0048] The programming controller is connected to the bacterial agent activation module and is used to control the stirring speed, activation temperature, dissolved oxygen concentration and bacterial agent dosage of the bacterial agent activation module;

[0049] The programmable controller is connected with the evaluation reactor, the water inlet unit and the low temperature circulation unit, and is used to control the stirring speed, dissolved oxygen concentration, temperature of the evaluation reactor, and the time and amount of water inlet and outlet of the evaluation reactor;

[0050] The programming controller is connected to the online monitoring unit and is used to transmit the monitoring signal of the online monitoring unit to the data processing center for processing and display the data results on the data display panel.

[0051] The present invention is helpful to screen high-quality nitrifying bacteria agents in low-temperature environments, screen out low-temperature and high-efficiency nitrifying bacteria agents suitable for different water qualities, and improve nitrogen removal efficiency in applications such as sewage treatment; the degree of self-control is high and the evaluation method is highly operable, so that ordinary personnel can quickly measure the nitrification performance of low-temperature nitrifying bacteria agents in batches.

[0052] The present invention also provides a method for evaluating the performance of a low-temperature nitrifying bacteria agent in the above device, comprising:

[0053] Bacterial agent activation: determine the bacterial growth curve and clarify the optimal conditions required for bacterial agent activation;

[0054] Bacterial agent component analysis: Analyze the COD content and bacterial component composition of the bacterial agent;

[0055] Bacterial agent nitrification performance test: The nitrification performance of the bacterial agent is tested through the operation process of preparation, activated sludge addition, substrate addition, bacterial agent addition and reaction start-up;

[0056] Reaction system activity analysis: analysis or determination of one or more of sludge concentration, sludge settling performance, biological phase, enzyme activity, adenosine triphosphate and specific oxygen consumption rate;

[0057] Result output and analysis: Use the online monitoring unit to monitor the system data in real time and transmit the system data to the data processing center. The data processing center automatically calculates the ammonia nitrogen removal rate, ammonia nitrogen degradation rate, nitrification rate and nitrite accumulation rate for performance evaluation.

[0058] According to the present invention, the optimal conditions include dissolved oxygen concentration of the bacterial agent, temperature, growth time, initial concentration and whether a carbon source is added;

[0059] Analysis of the bacterial composition of the inoculant includes:

[0060] Extract the whole genomic DNA of the bacterial agent;

[0061] Amplify the V3-V4 region of the whole genomic DNA;

[0062] The purified amplified product is sequenced, and the sequencing includes determining the amplified product, screening the sequence, purifying the sequence, splicing and filtering the sequence, identifying and removing invalid sequences, and classifying the sequence.

[0063] According to the present invention, the nitrification performance of the bacterial agent is tested through the operation process of preparation, activated sludge addition, substrate addition, bacterial agent addition and reaction start-up, including:

[0064] Preparation: Set the temperature of the evaluation reactor to 4-15℃ through the automatic control unit, set the water inlet and outlet time, water inlet and outlet volume, set the amount of bacterial agent added, and set the data monitoring frequency;

[0065] Dosing of activated sludge: Choose whether to add activated sludge according to the actual situation. If it is necessary to add activated sludge, add the actual activated sludge to the evaluation reactor;

[0066] Substrate addition: slowly add nitrogen-containing substrate to the evaluation reactor through the water inlet unit according to the set value, so that the initial substrate concentration reaches the predetermined value;

[0067] Bacterial agent addition: according to the evaluation requirements, add activated or unactivated bacterial agents to the evaluation reactor;

[0068] Reaction start: After the bacterial agent is added, the reaction timer is started. During the reaction, the water inlet unit continuously replenishes the substrate according to the substrate consumption to maintain a relatively stable substrate concentration.

[0069] Preferably, the sludge concentration is measured by an online suspended solids monitor;

[0070] Sludge settling performance was determined by sludge settling ratio and sludge volume index;

[0071] Biophase analysis observes the microbial population in the sludge through a microscope to assess the ecological health and treatment capacity of the sludge;

[0072] The enzyme activity determination is to analyze and monitor the biodegradability of sludge in real time by detecting the dehydrogenase activity in the sludge;

[0073] The determination of adenosine triphosphate is to measure the adenosine triphosphate content in each reaction time period by using an adenosine triphosphate detection kit to compare the microbial activity and biological threat index in the sludge at different periods;

[0074] The specific oxygen consumption rate is determined by controlling aeration through an automatic control unit to keep the dissolved oxygen in the system at a certain value, then recording the change curve of dissolved oxygen over time and calculating the k value, where k / MLSS is the specific oxygen consumption rate.

[0075] According to the present invention, the system data includes system ammonia nitrogen concentration, sludge concentration, nitrate nitrogen concentration and nitrite nitrogen concentration;

[0076] The data collection and monitoring frequency of the online monitoring unit is 30 minutes to 60 minutes per time;

[0077] The data processing center automatically calculates the ammonia nitrogen removal rate, ammonia nitrogen degradation rate, nitrification rate and nitrite accumulation rate, and performs performance evaluation including:

[0078] The data processing center calculates the ammonia nitrogen removal rate, ammonia nitrogen degradation rate, nitrification rate and nitrite accumulation rate based on the system data, and displays the results on the display panel;

[0079] Compare and analyze the evaluation results of this test with the preset standards or the evaluation results of other similar bacterial agents to determine whether the low-temperature nitrifying bacterial agent meets the requirements and the quality of its performance, so as to ensure the stability and effectiveness of the bacterial agent in practical applications;

[0080] The calculation formulas for ammonia nitrogen removal rate, ammonia nitrogen degradation rate, nitrification rate and nitrite accumulation rate are:

[0081] Ammonia nitrogen removal rate (%) = (initial ammonia nitrogen concentration - final ammonia nitrogen concentration) / initial ammonia nitrogen concentration × 100%;

[0082] Ammonia nitrogen degradation rate (mg N / L / h) = (initial ammonia nitrogen concentration - ammonia nitrogen concentration at a certain time) / reaction time;

[0083] Nitrification rate (mg N / L / h) = (nitrate nitrogen concentration at a certain moment - initial nitrate nitrogen concentration) / reaction time;

[0084] Nitrite nitrogen accumulation rate (%) = (nitrite nitrogen concentration at a certain moment / (initial ammonia nitrogen concentration - ammonia nitrogen concentration at a certain moment)) × 100%.

[0085] The present invention will be described in more detail below by way of examples.

[0086] Embodiment 1:

[0087] like Figure 1 As shown, this embodiment proposes a low-temperature nitrifying bacteria agent performance evaluation system, including: a water inlet unit, a bacteria agent activation module, an evaluation reactor, a low-temperature circulation unit, an online monitoring unit, and an automatic control unit;

[0088] The water inlet unit is connected to the bacterial agent activation module and the evaluation reactor respectively, and the unit includes a water inlet tank, a metering pump and a delivery pipeline; the water inlet tank is used to store the sewage required for the evaluation; the metering pump can accurately deliver the sewage according to the set flow rate to ensure the stability of the substrate concentration during the reaction process;

[0089] The bacterial agent activation module is used to evaluate the activation and recovery of the bacterial agent and accurately put the low-temperature nitrifying bacterial agent into the evaluation reactor; it is equipped with a heating module, a stirring device, an aeration module and a high-precision metering device, which can realize the revival and quantitative delivery of the bacterial agent, ensuring that the initial inoculation amount of the bacterial agent is accurate and consistent in each evaluation;

[0090] The evaluation reactor is used to evaluate the ammonia nitrogen removal capacity of the bacterial agent under low temperature conditions. It is equipped with a stirring device and an aeration module. The reactor is equipped with multiple inlet and outlet valves, which are used for water inlet and outlet and sampling. The reactor is connected to a low-temperature water bath, and the outer layer is equipped with insulation. A sampling port is reserved on the top of the reactor for the installation of online instruments. The reactor is cylindrical, and a low-temperature circulation layer is set on the outside.

[0091] The low temperature circulation unit is connected to the reactor and is used for the temperature control of the reactor. The temperature range of the reaction system can be controlled within the range of -5℃ to 90℃.

[0092] The online monitoring unit is connected to the evaluation reactor and the automatic control unit, and includes a temperature sensor, pH, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, sludge concentration, and an online monitor for dissolved oxygen, which are arranged at a preset position of the reactor; the online monitoring signals of the temperature sensor, pH, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, sludge concentration, and dissolved oxygen are sent to the automatic control unit;

[0093] The automatic control unit includes a programmable controller, a memory, a data processing center, and a data display panel; the controller is connected to the microbial agent activation module to control its stirring speed, activation temperature, dissolved oxygen concentration, and microbial agent dosage; the controller is connected to the evaluation reactor and the low-temperature circulation unit to control the reactor stirring speed, dissolved oxygen concentration, temperature, and the time and amount of water inlet and outlet of the reactor; the controller is connected to the online monitoring unit, and the monitoring signal of the online monitoring unit will be transmitted to the data processing center for processing, and the data results will be displayed on the data display panel;

[0094] The aeration device is an aeration unit with a flow meter, which can control the aeration volume in real time and the dissolved oxygen range can be controlled to 0-8mg / L;

[0095] The stirring device is a stirrer with controllable stirring speed, and the stirrer is a two-blade paddle stirrer which can fully and evenly mix the sewage and the sludge.

[0096] like Figure 2 As shown, this embodiment provides a method for evaluating the performance of a low-temperature nitrifying bacteria agent, comprising the following steps:

[0097] (1) The sewage from the primary sedimentation tank and the sludge from the aerobic tank of a sewage treatment plant in Beijing were taken as the test subjects for the low-temperature nitrifying bacteria agent. After the sludge samples were taken back, they were placed in a 4°C refrigerator for 2-3 days to reduce the sludge activity. The sewage was cooled for 1 day to achieve a simulated low-temperature environment.

[0098] (2) Bacterial agent activation: Two low-temperature nitrifying bacterial agents were selected, and the bacterial agent activation module was equipped with a test bacterial agent with a concentration of 100 g / L. Aeration was performed to maintain the dissolved oxygen at 1 mg / L at room temperature for about 6 hours;

[0099] (3) Analysis of bacterial composition: COD content detection; Bacterial composition analysis, COD content analysis of bacterial agent is shown in Table 1;

[0100] Table 1 Analysis of COD content of bacterial agent

[0101]

[0102] From Table 1, it can be seen that the COD concentration of the two bacterial agents added to the 5L system is less than 0.1, which can be ignored;

[0103] Figure 3 The bacterial genus composition of the two bacterial agents is analyzed. Bacillus is the main bacterial genus of the two bacterial agents, occupying a dominant position.

[0104] (4) Test of nitrification performance of bacterial agent: The test of nitrification performance of bacterial agent is as follows: ① Preparation: cool the circulating water and the system, set the time to 12h, and control the system temperature at 6-7℃; mix the cooled sewage and activated sludge in the storage device so that the system volume is 5L and the sludge concentration is maintained at 5000mg / L; set the sewage inlet flow rate to 10mL / min; set the bacterial agent dosage to 500mg / L; set the data monitoring frequency to times / h; ② Activated sludge addition: the sludge concentration is maintained at 5000mg / L; ③ Substrate addition: the sewage inlet flow rate of the primary sedimentation tank of the sewage treatment plant is 10mL / min; ④ Bacterial agent addition: the bacterial agent dosage is 500mg / L of the activated bacterial agent; ⑤ Reaction start: after the bacterial agent is added, start the reaction timer. During the reaction, the water inlet unit continuously replenishes the substrate according to the substrate consumption to maintain the relative stability of the substrate concentration;

[0105] (5) Analysis of the activity of the reaction system: The concentration of adenosine triphosphate (ATP) was selected to characterize the activity of the system. The ATP concentration can be used to evaluate the activity of microorganisms under different environmental influences. The QuenchGone21 Wastewater (QG21W TM ) The reagent kit detects total ATP and extracellular ATP in the system, and calculates the intracellular ATP mass concentration and the biological threat index (BSI, Biological Stress Index);

[0106] According to the data in Table 2, after adding the bacterial agent, the cATP content of the system increased and the biological threat index decreased, indicating that the activity of the system was enhanced;

[0107] Table 2 Intracellular ATP concentration and BSI index of the system

[0108]

[0109] (6) Result output and analysis:

[0110] Data at different times were selected to calculate ammonia nitrogen removal rate, ammonia nitrogen degradation rate, nitrification rate, and nitrite accumulation rate. The comparative calculation results are shown in Tables 3 to 9 respectively;

[0111] Table 3 Ammonia nitrogen concentrations at different time periods for different low-temperature nitrifying bacteria

[0112]

[0113] Table 4 Nitrate nitrogen concentrations at different time periods using different low-temperature nitrifying bacteria

[0114]

[0115] Table 5 Nitrite nitrogen concentrations at different time periods using different low-temperature nitrifying bacteria

[0116]

[0117]

[0118] Table 6 Ammonia nitrogen removal rate of different low-temperature nitrifying bacteria in different time periods (%)

[0119]

[0120] Table 7 Ammonia nitrogen degradation rate of different low-temperature nitrifying bacteria at different time periods (mg N / L / h)

[0121]

[0122] Table 8 Nitrification rate of different low-temperature nitrifying bacteria in different time periods (mg N / L / h)

[0123]

[0124] Table 9 Nitrite accumulation rate at different time periods of different low-temperature nitrifying bacteria (%)

[0125]

[0126] From the calculated data in Tables 3 to 9, we can see the ammonia nitrogen removal capacity, nitrification capacity, and nitrite accumulation of bacterial agent 1 and bacterial agent 2 in sewage treatment under low temperature environment. Combined with the activity analysis of the reaction system in Table 2, bacterial agent 2 is a bacterial agent with good low-temperature nitrification ability.

[0127] The low-temperature nitrifying bacteria agent performance evaluation system proposed in the embodiment of the present invention has the advantages of reasonable structural design, reliable and stable operation, high degree of automation and intelligence, and wide application range. It can simulate the actual low-temperature environment, accurately and efficiently evaluate and assess the performance of the low-temperature nitrifying bacteria agent, so as to screen out high-efficiency nitrifying bacteria agents suitable for low-temperature applications in different water qualities.

[0128] The embodiments of the present invention have been described above, and the above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and changes will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments.

Claims

1. A low-temperature nitrifying bacteria agent performance evaluation system, characterized in that: It includes water inlet unit, bacterial agent activation module, evaluation reactor, low temperature circulation unit, online monitoring unit and automatic control unit; The water inlet unit is connected to the bacterial agent activation module and the evaluation reactor respectively; The bacterial agent activation module is used to evaluate the activation and recovery of the bacterial agent and accurately put the low-temperature nitrifying bacterial agent into the evaluation reactor; The evaluation reactor is connected to the low-temperature circulation unit and is used to evaluate the ability of the bacterial agent to remove ammonia nitrogen under low-temperature conditions; The low temperature circulation unit is used to control the temperature of the evaluation reactor; The online monitoring unit is connected to the evaluation reactor and the automatic control unit respectively, and is used to monitor the evaluation reactor and send a monitoring signal to the automatic control unit.

2. The system according to claim 1, characterized in that The water inlet unit includes a water inlet tank, a metering pump and a delivery pipeline; The water inlet tank is used to store sewage or water for evaluation; The metering pump is used to accurately deliver sewage or experimental water according to the set flow rate to ensure the stability of the substrate concentration during the reaction process; The microbial agent activation module is equipped with a heating module, a stirring device, an aeration module and a high-precision metering device, which are used to realize the revival and quantitative delivery of the microbial agent, ensuring that the initial inoculation amount of the microbial agent is accurate and consistent in each evaluation.

3. The system according to claim 1, characterized in that The evaluation reactor is cylindrical, with a low-temperature circulation layer and a heat preservation layer arranged on the outside in sequence, a sampling port reserved on the top for the installation of online instruments, a stirring device and an aeration module arranged inside, and multiple water inlet and outlet valves arranged for water inlet and outlet and sampling respectively; The low temperature circulation unit is connected to the low temperature circulation layer, and controls the temperature range of the reaction system to be -5°C to 90°C; The low temperature circulation unit is a low temperature water bath or a semiconductor refrigeration sheet; The aeration module is an aeration unit with a flow meter, which is used to control the aeration volume in real time, and the dissolved oxygen range can be controlled to be 0-8 mg / L; The stirring device is a stirrer with controllable stirring speed, and the stirrer is a two-blade paddle stirrer, which is used to fully and evenly mix the sewage and the sludge.

4. The system according to claim 1, characterized in that The online monitoring unit includes a temperature sensor, a pH, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, a sludge concentration meter, and an online monitor for dissolved oxygen, which are arranged at a preset position of the evaluation reactor; The online monitoring signals of the temperature sensor, pH, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, sludge concentration meter and dissolved oxygen are sent to the automatic control unit.

5. The system according to claim 1, characterized in that The automatic control unit includes a programmable controller, a memory, a data processing center and a data display panel; The programming controller is connected to the bacterial agent activation module and is used to control the stirring speed, activation temperature, dissolved oxygen concentration and bacterial agent dosage of the bacterial agent activation module; The programmable controller is connected to the evaluation reactor, the water inlet unit and the low-temperature circulation unit, and is used to control the stirring speed, dissolved oxygen concentration, temperature of the evaluation reactor, and the time and amount of water inlet and outlet of the evaluation reactor; The programming controller is connected to the online monitoring unit and is used to transmit the monitoring signal of the online monitoring unit to the data processing center for processing and display the data result on the data display panel.

6. A method for evaluating the performance of a low-temperature nitrifying bacteria agent, characterized in that: include: Bacterial agent activation: determine the bacterial growth curve and clarify the optimal conditions required for bacterial agent activation; Bacterial agent component analysis: Analyze the COD content and bacterial component composition of the bacterial agent; Bacterial agent nitrification performance test: The nitrification performance of the bacterial agent is tested through the operation process of preparation, activated sludge addition, substrate addition, bacterial agent addition and reaction start-up; Reaction system activity analysis: analysis or determination of one or more of sludge concentration, sludge settling performance, biological phase, enzyme activity, adenosine triphosphate and specific oxygen consumption rate; Result output and analysis: The system data is monitored in real time using an online monitoring unit, and the system data is transmitted to a data processing center, through which the ammonia nitrogen removal rate, ammonia nitrogen degradation rate, nitrification rate, and nitrite accumulation rate are automatically calculated for performance evaluation.

7. The system according to claim 6, characterized in that The optimal conditions include dissolved oxygen concentration of the bacterial agent, temperature, growth time, initial concentration and whether a carbon source is added; Analysis of the bacterial composition of the inoculant includes: Extract the whole genomic DNA of the bacterial agent; amplifying the V3-V4 region of the whole genomic DNA; The purified amplified product is sequenced, and the sequencing includes determining the amplified product, screening the sequence, purifying the sequence, splicing and filtering the sequence, identifying and removing invalid sequences, and classifying the sequence.

8. The system according to claim 6, characterized in that The nitrification performance of the bacterial agent is tested through the operation process of preparation, activated sludge addition, substrate addition, bacterial agent addition and reaction start-up, including: Preparation: Set the temperature of the evaluation reactor to 4-15℃ through the automatic control unit, set the water inlet and outlet time, water volume, bacterial agent dosage, and data monitoring frequency; Adding activated sludge: whether to add activated sludge is required according to the actual situation. If it is required, the activated sludge actually used is added to the evaluation reactor; Substrate addition: slowly adding nitrogen-containing substrate into the evaluation reactor according to the set value through the water inlet unit, so that the initial substrate concentration reaches a predetermined value; Adding bacterial agent: according to the evaluation requirements, adding activated or unactivated bacterial agent to the evaluation reactor; Reaction start: After the bacterial agent is added, the reaction timer is started. During the reaction, the water inlet unit continuously replenishes the substrate according to the substrate consumption to maintain a relatively stable substrate concentration.

9. The system according to claim 6, characterized in that The sludge concentration is measured by an online suspended matter monitor; The sludge settling performance is measured by sludge settling ratio and sludge volume index; The analysis of the biophase is to observe the microbial population in the sludge through a microscope to evaluate the ecological health status and treatment capacity of the sludge; The enzyme activity is determined by detecting the dehydrogenase activity in the sludge, analyzing and real-time monitoring the biodegradability of the sludge; The determination of adenosine triphosphate is to measure the adenosine triphosphate content in each reaction time period by using an adenosine triphosphate detection kit to compare the microbial activity and biological threat index in the sludge at different periods; The specific oxygen consumption rate is determined by controlling aeration through an automatic control unit to keep the dissolved oxygen in the system at a certain value, then recording the change curve of dissolved oxygen over time and obtaining the k value, wherein k / MLSS is the specific oxygen consumption rate.

10. The system according to claim 6, characterized in that The system data include system ammonia nitrogen concentration, sludge concentration, nitrate nitrogen concentration and nitrite nitrogen concentration; The data collection and monitoring frequency of the online monitoring unit is 30 minutes to 60 minutes per time; The data processing center automatically calculates the ammonia nitrogen removal rate, ammonia nitrogen degradation rate, nitrification rate and nitrite accumulation rate, and performs performance evaluation including: The data processing center calculates the ammonia nitrogen removal rate, ammonia nitrogen degradation rate, nitrification rate and nitrite accumulation rate based on the system data, and displays the results on the display panel; Compare and analyze the evaluation results of this test with the preset standards or the evaluation results of other similar bacterial agents to determine whether the low-temperature nitrifying bacterial agent meets the requirements and the quality of its performance, so as to ensure the stability and effectiveness of the bacterial agent in practical applications; The calculation formulas for the ammonia nitrogen removal rate, ammonia nitrogen degradation rate, nitrification rate and nitrite accumulation rate are respectively: Ammonia nitrogen removal rate (%) = (initial ammonia nitrogen concentration - final ammonia nitrogen concentration) / initial ammonia nitrogen concentration × 100%; Ammonia nitrogen degradation rate (mg N / L / h) = (initial ammonia nitrogen concentration - ammonia nitrogen concentration at a certain time) / reaction time; Nitrification rate (mg N / L / h) = (nitrate nitrogen concentration at a certain moment - initial nitrate nitrogen concentration) / reaction time; Nitrite nitrogen accumulation rate (%) = (nitrite nitrogen concentration at a certain moment / (initial ammonia nitrogen concentration - ammonia nitrogen concentration at a certain moment)) × 100%.

Images