Stable optical collection system for flow cytometer

Abstract

The invention discloses a stable optical collection system for a flow cytometer, which comprises a dual-laser optical collection system, an optical data acquisition system, an optical data storage and query system, an optical data abnormity warning system, a central control system, an optical data detection cloud service system, a data uploading system and a terminal management system, the double-laser optical collection system is connected with the optical data acquisition system, and the optical data acquisition system is connected with the optical data storage and query system. According to the stable optical collection system for the flow cytometer, provided by the invention, double lasers adopt at most 8 fluorescence channels and 10 detection channels, the stable optical collection system is arranged, spatial three-dimensional separation excitation and spatial three-dimensional separation receiving are adopted, crosstalk does not exist between the lasers, compensation is reduced, and a solid-state laser is adopted; and the collected data is sorted, analyzed, stored, inquired and subjected to terminal management, so that the optical collection and detection efficiency of the flow cytometer is improved.

Description

Technical Field

[0001] The invention relates to the field of flow cytometers, and in particular to a stable optical collection system for flow cytometers. Background Art

[0002] The stable optical collection system for flow cytometer is a method for performing optical detection inside the flow cytometer. The beam stabilization system is often used to compensate for vibration, shock, thermal drift or other unwanted fluctuations in the direction of the laser beam. It is suitable for situations where laser fluctuations or movement of optical components occur, but requires high precision and stability in the direction of the beam. With the continuous development of science and technology, people have higher and higher requirements for the manufacturing process of the optical collection system for flow cytometers.

[0003] The existing optical collection system for flow cytometers has certain drawbacks when in use. First, there may be crosstalk between lasers in the existing optical collection system for flow cytometers when in use, which requires compensation, reducing the detection accuracy of the flow cytometer and being inconvenient for people to use. In addition, the existing optical collection system for flow cytometers cannot conveniently perform optical data acquisition and management, which brings certain adverse effects to people's use process. For this reason, we propose a stable optical collection system for flow cytometers. Summary of the invention

[0004] Technical problems solved: In view of the shortcomings of the prior art, the present invention provides a stable optical collection system for a flow cytometer, which is equipped with dual lasers and uses up to 8 fluorescence channels and 10 detection channels. A stable optical collection system is set up, and spatial stereo separation excitation and spatial stereo separation reception are adopted. There is no crosstalk between lasers, and solid-state lasers are used to reduce compensation. The collected data can be sorted, analyzed, stored, queried and terminally managed, thereby improving the optical collection and detection efficiency of the flow cytometer, which can effectively solve the problems in the background technology.

[0005] Technical solution: To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: a stable optical collection system for a flow cytometer, comprising a dual-laser optical collection system, an optical data acquisition system, an optical data storage query system, an optical data abnormality warning system, a central control system, an optical data detection cloud service system, a data upload system and a terminal management system, the dual-laser optical collection system is connected to the optical data acquisition system, the optical data acquisition system is connected to the optical data storage query system, the optical data storage query system is connected to the optical data abnormality warning system and the central control system, the central control system is connected to the optical data detection cloud service system, the optical data detection cloud service system is connected to the data upload system, and the data upload system is connected to the terminal management system.

[0006] As a preferred technical solution of the present application, the dual laser optical collection system includes 8 fluorescence channel modules, 10 detection channel modules, a spatial stereo separation excitation module, a spatial stereo separation receiving module, a solid-state laser module, a photoelectric detection and collection module and an integrated stable collection module. The 8 fluorescence channel modules, 10 detection channel modules, the spatial stereo separation excitation module, the spatial stereo separation receiving module, the solid-state laser module and the photoelectric detection and collection module are all connected to the integrated stable collection module.

[0007] As a preferred technical solution of the present application, the optical data acquisition system includes a data receiving module, a multi-channel module, a data acquisition module, a data input module, a data sorting module, a microprocessor, a data storage module, a data encryption module and a data output module. The data receiving module is connected to the multi-channel module, the multi-channel module is connected to the data acquisition module, the data acquisition module is connected to the data input module, the data input module is connected to the data sorting module, the data sorting module is connected to the microprocessor, and the microprocessor is connected to the data storage module and the data encryption module.

[0008] As a preferred technical solution of the present application, the optical data anomaly warning system includes a data verification module, a data configuration module, a data analysis module, a data monitoring module, a data alarm module, an abnormal display module and an abnormal alarm module. The data monitoring module is connected to the data configuration module and the data alarm module, the data configuration module is connected to the data verification module and the data analysis module, and the data alarm module is connected to the abnormal display module and the abnormal alarm module.

[0009] As a preferred technical solution of the present application, the optical data storage query system includes a database, a data decryption module, a data query module and an access module. The database is connected to the data decryption module, the data decryption module is connected to the data query module, and the data query module is connected to the access module.

[0010] As a preferred technical solution of the present application, the output ends of the 8-fluorescence channel module, 10 detection channel module, spatial stereo separation excitation module, spatial stereo separation receiving module, solid-state laser module and photoelectric detection and collection module are all connected to the input end of the integrated stable collection module.

[0011] As a preferred technical solution of the present application, the output end of the data receiving module is connected to the input end of the multi-channel module, the output end of the multi-channel module is connected to the input end of the data acquisition module, the output end of the data acquisition module is connected to the input end of the data input module, the output end of the data input module is connected to the input end of the data sorting module, the output end of the data sorting module is connected to the input end of the microprocessor, and the output end of the microprocessor is connected to the input end of the data storage module and the data encryption module.

[0012] As a preferred technical solution of the present application, the output ends of the data verification module and the data analysis module are connected to the input end of the data configuration module, the output end of the data configuration module is connected to the input end of the data monitoring module, the output end of the data monitoring module is connected to the input end of the data alarm module, and the output end of the data alarm module is connected to the input end of the abnormal display module and the abnormal alarm module.

[0013] Beneficial effects: Compared with the prior art, the present invention provides a stable optical collection system for a flow cytometer, which has the following beneficial effects: the stable optical collection system for a flow cytometer is configured with dual lasers using up to 8 fluorescence channels and 10 detection channels, a stable optical collection system is set up, spatial stereo separation excitation and spatial stereo separation reception are used, there is no crosstalk between lasers, solid-state lasers are used to reduce compensation, and the collected data are sorted, analyzed, stored, queried and terminally managed, thereby improving the optical collection and detection efficiency of the flow cytometer, the output ends of the 8 fluorescence channel modules, 10 detection channel modules, spatial stereo separation excitation modules, spatial stereo separation receiving modules, solid-state laser modules and photoelectric detection and collection modules are all connected to the input end of the integrated stable collection module, the multi-channel integration performs optical detection and collection, spatial stereo separation excitation and spatial stereo separation reception are used, there is no crosstalk between different laser channels, no compensation is required, high stability after vibration is maintained, and detection accuracy is improved, the entire stable optical collection system for the flow cytometer has a simple structure, is easy to operate, and has a better effect than the traditional method. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The figure is a schematic diagram of the overall structure of a stable optical collection system for a flow cytometer according to the present invention.

[0015] Figure 2 The present invention is a schematic structural diagram of a dual-laser optical collection system in a stable optical collection system for a flow cytometer.

[0016] Figure 3 The present invention is a schematic diagram of the structure of a data acquisition system in a stable optical collection system for a flow cytometer.

[0017] Figure 4 The present invention is a schematic structural diagram of a data storage and query system in a stable optical collection system for a flow cytometer.

[0018] Figure 5 The present invention is a schematic structural diagram of an optical data abnormality warning system in a stable optical collection system for a flow cytometer. DETAILED DESCRIPTION

[0019] The technical scheme of the present invention will be clearly and completely described below in conjunction with the accompanying drawings and specific embodiments, but it will be understood by those skilled in the art that the following described embodiments are part of embodiments of the present invention, rather than all embodiments, and are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present invention. If specific conditions are not specified in the embodiments, they are carried out according to normal conditions or conditions recommended by the manufacturer. If the manufacturer is not specified in the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0020] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0021] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0022] like Figure 1-5As shown, a stable optical collection system for a flow cytometer includes a dual-laser optical collection system, an optical data acquisition system, an optical data storage query system, an optical data abnormality warning system, a central control system, an optical data detection cloud service system, a data upload system and a terminal management system. The dual-laser optical collection system is connected to the optical data acquisition system, the optical data acquisition system is connected to the optical data storage query system, the optical data storage query system is connected to the optical data abnormality warning system and the central control system, the central control system is connected to the optical data detection cloud service system, the optical data detection cloud service system is connected to the data upload system, and the data upload system is connected to the terminal management system. The dual lasers are configured to use up to 8 fluorescence channels and 10 detection channels, a stable optical collection system is set up, spatial stereo separation excitation and spatial stereo separation reception are used, there is no crosstalk between lasers, solid-state lasers are used to reduce compensation, and the collected data are sorted, analyzed, stored, queried and managed by terminals, thereby improving the optical collection and detection efficiency of the flow cytometer.

[0023] Furthermore, the dual-laser optical collection system includes an 8-fluorescence channel module, a 10-detection channel module, a spatial stereo separation excitation module, a spatial stereo separation receiving module, a solid-state laser module, a photoelectric detection collection module and an integrated stable collection module. The 8-fluorescence channel module, the 10-detection channel module, the spatial stereo separation excitation module, the spatial stereo separation receiving module, the solid-state laser module and the photoelectric detection collection module are all connected to the integrated stable collection module.

[0024] Furthermore, the optical data acquisition system includes a data receiving module, a multi-channel module, a data acquisition module, a data input module, a data sorting module, a microprocessor, a data storage module, a data encryption module and a data output module. The data receiving module is connected to the multi-channel module, the multi-channel module is connected to the data acquisition module, the data acquisition module is connected to the data input module, the data input module is connected to the data sorting module, the data sorting module is connected to the microprocessor, and the microprocessor is connected to the data storage module and the data encryption module.

[0025] Furthermore, the optical data anomaly warning system includes a data verification module, a data configuration module, a data analysis module, a data monitoring module, a data alarm module, an abnormal display module and an abnormal alarm module. The data monitoring module is connected to the data configuration module and the data alarm module, the data configuration module is connected to the data verification module and the data analysis module, and the data alarm module is connected to the abnormal display module and the abnormal alarm module.

[0026] Furthermore, the optical data storage query system includes a database, a data decryption module, a data query module and an access module. The database is connected to the data decryption module, the data decryption module is connected to the data query module, and the data query module is connected to the access module.

[0027] Furthermore, the output ends of the 8-fluorescence channel module, the 10-detection channel module, the spatial stereo separation excitation module, the spatial stereo separation receiving module, the solid-state laser module and the photoelectric detection collection module are all connected to the input end of the integrated stable collection module.

[0028] Furthermore, the output end of the data receiving module is connected to the input end of the multi-channel module, the output end of the multi-channel module is connected to the input end of the data acquisition module, the output end of the data acquisition module is connected to the input end of the data input module, the output end of the data input module is connected to the input end of the data sorting module, the output end of the data sorting module is connected to the input end of the microprocessor, and the output end of the microprocessor is connected to the input end of the data storage module and the data encryption module.

[0029] Furthermore, the output ends of the data verification module and the data analysis module are connected to the input end of the data configuration module, the output end of the data configuration module is connected to the input end of the data monitoring module, the output end of the data monitoring module is connected to the input end of the data alarm module, and the output end of the data alarm module is connected to the input end of the abnormal display module and the abnormal alarm module.

[0030] Working principle: The output ends of the 8-fluorescence channel module, 10 detection channel module, spatial stereo separation excitation module, spatial stereo separation receiving module, solid-state laser module and photoelectric detection and collection module are all connected to the input end of the integrated stable collection module. Multi-channel integration is used for optical detection and collection, and spatial stereo separation excitation and spatial stereo separation reception are used. There is no crosstalk between different laser channels and no compensation is required. High stability after vibration is maintained to improve detection accuracy. The dual laser configuration uses up to 8 fluorescence channels and 10 detection channels. A stable optical collection system is set up, using spatial stereo separation excitation and spatial stereo separation reception. There is no crosstalk between lasers. Solid-state lasers are used to reduce compensation. The collected data is organized, analyzed, stored, queried and terminally managed to improve the optical collection and detection efficiency of the flow cytometer.

[0031] It should be noted that, in this article, relational terms such as first and second (number one, number two), etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "including one..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0032] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A stable optical collection system for a flow cytometer, comprising a dual-laser optical collection system, an optical data acquisition system, an optical data storage and query system, an optical data abnormality warning system, a central control system, an optical data detection cloud service system, a data upload system and a terminal management system, characterized in that: The dual-laser optical collection system is connected to the optical data acquisition system, the optical data acquisition system is connected to the optical data storage query system, the optical data storage query system is connected to the optical data abnormality warning system and the central control system, the central control system is connected to the optical data detection cloud service system, the optical data detection cloud service system is connected to the data upload system, and the data upload system is connected to the terminal management system.

2. A stable optical collection system for a flow cytometer according to claim 1, characterized in that: The dual-laser optical collection system includes 8 fluorescence channel modules, 10 detection channel modules, a spatial stereo separation excitation module, a spatial stereo separation receiving module, a solid-state laser module, a photoelectric detection collection module and an integrated stable collection module. The 8 fluorescence channel modules, 10 detection channel modules, the spatial stereo separation excitation module, the spatial stereo separation receiving module, the solid-state laser module and the photoelectric detection collection module are all connected to the integrated stable collection module.

3. A stable optical collection system for a flow cytometer according to claim 1, characterized in that: The optical data acquisition system includes a data receiving module, a multi-channel module, a data acquisition module, a data input module, a data sorting module, a microprocessor, a data storage module, a data encryption module and a data output module. The data receiving module is connected to the multi-channel module, the multi-channel module is connected to the data acquisition module, the data acquisition module is connected to the data input module, the data input module is connected to the data sorting module, the data sorting module is connected to the microprocessor, and the microprocessor is connected to the data storage module and the data encryption module.

4. A stable optical collection system for a flow cytometer according to claim 1, characterized in that: The optical data anomaly warning system includes a data verification module, a data configuration module, a data analysis module, a data monitoring module, a data alarm module, an abnormal display module and an abnormal alarm module. The data monitoring module is connected to the data configuration module and the data alarm module, the data configuration module is connected to the data verification module and the data analysis module, and the data alarm module is connected to the abnormal display module and the abnormal alarm module.

5. A stable optical collection system for a flow cytometer according to claim 1, characterized in that: The optical data storage query system comprises a database, a data decryption module, a data query module and an access module. The database is connected to the data decryption module, the data decryption module is connected to the data query module, and the data query module is connected to the access module.

6. A stable optical collection system for a flow cytometer according to claim 2, characterized in that: The output ends of the 8-fluorescence channel module, the 10-detection channel module, the spatial stereo separation excitation module, the spatial stereo separation receiving module, the solid-state laser module and the photoelectric detection collection module are all connected to the input end of the integrated stable collection module.

7. A stable optical collection system for a flow cytometer according to claim 3, characterized in that: The output end of the data receiving module is connected to the input end of the multi-channel module, the output end of the multi-channel module is connected to the input end of the data acquisition module, the output end of the data acquisition module is connected to the input end of the data input module, the output end of the data input module is connected to the input end of the data sorting module, the output end of the data sorting module is connected to the input end of the microprocessor, and the output end of the microprocessor is connected to the input ends of the data storage module and the data encryption module.

8. A stable optical collection system for a flow cytometer according to claim 4, characterized in that: The output ends of the data verification module and the data analysis module are both connected to the input end of the data configuration module, the output end of the data configuration module is connected to the input end of the data monitoring module, the output end of the data monitoring module is connected to the input end of the data alarm module, and the output end of the data alarm module is connected to the input end of the abnormal display module and the abnormal alarm module.

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