Aromatic molecular imprinting fluorescence sensor preparation method and fluorescence intensity detection device
By preparing an aromatic molecular imprinted fluorescence sensor at room temperature and combining it with a fluorescence intensity detection device, the problem of difficulty in quickly and accurately detecting polycyclic aromatic hydrocarbons in the prior art is solved, and a low-cost and high-accuracy detection effect is achieved.
Patent Information
- Application Number
- CN202411820265.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-11
- Publication Date
- 2025-03-11
AI Technical Summary
The prior art is difficult to quickly and accurately detect the content of polycyclic aromatic hydrocarbons in the environment at room temperature, and the traditional methods are costly to operate, have high energy consumption and are inconvenient for rapid on-site screening.
Using the preparation method of aromatic molecular imprinting fluorescence sensor, a fluorescent probe structure with excellent fluorescence characteristics and reusable is prepared by embedding carbon quantum dots into aromatic molecular polymers, and the fluorescence intensity detection device is used for detection.
It realizes the rapid and accurate detection of the content of aromatic molecules in the environment at room temperature, and the detection device is simple in structure, low in cost, and has high accuracy and repeatability.
Smart Images
Figure CN119595604A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of fluorescence detection, and in particular to a method for preparing an aromatic hydrocarbon molecular imprinting fluorescence sensor and a fluorescence intensity detection device. Background Art
[0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of organic pollutants widely present in the environment. They have attracted much attention due to their unique chemical properties and biological activities. This class of compounds has been shown to have significant carcinogenic, teratogenic and mutagenic effects, commonly referred to as the "three-cause" effect. Due to these characteristics, the presence of PAHs not only threatens the health and stability of the ecosystem, but also seriously endangers human life and safety. Considering their semi-volatile characteristics, PAHs can be transmitted to distant places through the air; at the same time, they are easily accumulated in organisms and are difficult to decompose or degrade naturally. This means that once released into the environment, PAHs may have long-term or even permanent effects on the ecological environment. In view of this, how to effectively monitor and control the content of PAHs in the environment has become an urgent problem to be solved.
[0003] So far, the most commonly used technical means in practical applications include but are not limited to high performance liquid chromatography, gas chromatography, thin layer scanning analysis and paper chromatography. Although these methods can provide relatively accurate results, they also have some obvious disadvantages: first, the high operating costs make large-scale applications unrealistic; second, most detection processes require the support of high temperature conditions, which not only increases the difficulty of the experiment but also increases the energy consumption level; finally, for situations that require rapid on-site screening, the above traditional methods are obviously not convenient and efficient enough. In recent years, new sensing technologies based on the principle of fluorescence have brought new hope to this field. Unfortunately, some of the related products currently available on the market are often complex in structure and expensive.
[0004] Therefore, it is necessary to invent a method and device for quickly identifying aromatic molecules at room temperature. Summary of the invention
[0005] In view of the above existing technical problems, the present invention aims to invent a method for preparing an aromatic hydrocarbon molecular imprinting fluorescence sensor and a fluorescence intensity detection device, which can quickly and accurately detect the content of aromatic hydrocarbon molecules in the environment in real time.
[0006] The present invention discloses a method for preparing an aromatic hydrocarbon molecular imprinting fluorescence sensor, comprising the following steps:
[0007] Preparation of S1 mixed solution: acetonitrile is added into the reactor, and then aromatic molecules and α-methylacrylic acid are added into the acetonitrile, and after the addition is completed, ultrasonic vibration treatment is performed to obtain a mixed solution;
[0008] S2 Preparation of aromatic molecular polymer: Add oxalic acid dimethyl acrylate, azobisisobutyronitrile and carbon quantum dots to the mixed solution obtained in step S1, replace oxygen with nitrogen after the addition is completed, heat in a water bath for reaction, and after the reaction is completed, vacuum dry the reaction solution to obtain an aromatic molecular polymer;
[0009] S3 Preparation of aromatic hydrocarbon molecular imprinting fluorescence sensor: The aromatic hydrocarbon molecular polymer obtained in step S2 is eluted with methanol acetic acid eluent until the ultraviolet absorption spectrometer detects that there is no ultraviolet absorption peak of aromatic hydrocarbon molecules in the eluent, thereby obtaining an aromatic hydrocarbon molecular imprinting fluorescence sensor.
[0010] Preferably, in the step of preparing the S1 mixed solution, the molar ratio of the aromatic hydrocarbon molecules to α-methylacrylic acid is 1:(3.9-4.1); the molar ratio of the aromatic hydrocarbon molecules to acetonitrile is 1:(19-58).
[0011] Preferably, in the S2 aromatic hydrocarbon molecule polymer preparation step, the molar ratio of oxalic acid dimethacrylate to aromatic hydrocarbon molecules is (5-15):1.
[0012] Preferably, in the S2 aromatic molecular polymer preparation step, the molar ratio of the azobisisobutyronitrile to the aromatic molecules is 1:(0.7-0.9); the mass ratio of the carbon quantum dots to the azobisisobutyronitrile is 1:(3.9-4.1).
[0013] Preferably, in the S2 aromatic molecular polymer preparation step, the water bath heating temperature is 50-70°C.
[0014] Preferably, in the step of preparing the aromatic hydrocarbon molecular imprinting fluorescent sensor, the volume fraction of the acetic acid is 5-15%.
[0015] The present invention also discloses a fluorescence intensity detection device for an aromatic hydrocarbon molecular imprinting fluorescence sensor, wherein a shell is arranged above a sample storage pool, a dustproof glass is arranged inside one end of the shell close to the sample storage pool, a first cavity is arranged directly above the dustproof glass, an ultraviolet light absorption filter, a second focusing lens, a photodiode, and a light-shielding box are arranged in sequence from bottom to top at one end of the first cavity close to the bottom of the shell, a second cavity is arranged in the oblique direction of the dustproof glass, and an interference filter, a first focusing lens, and an ultraviolet light source are arranged in sequence from bottom to top at one end of the second cavity close to the bottom of the shell.
[0016] Preferably, the sample storage pool is provided with a groove directly below the dustproof glass for storing the sample to be tested.
[0017] Preferably, an ultraviolet light absorbing filter is arranged at one end of the first cavity close to the dust-proof glass, a light-shielding box is arranged outside the first cavity at an end away from the dust-proof glass, the photodiode is arranged inside the first cavity at an end away from the dust-proof glass, and the second focusing lens is arranged between the photodiode and the ultraviolet light absorbing filter.
[0018] Preferably, the interference filter is arranged at one end of the second cavity close to the dust-proof glass, the ultraviolet light source is arranged at one end of the second cavity away from the dust-proof glass, and the first focusing lens is arranged between the interference filter and the ultraviolet light source; the ultraviolet light source is wrapped with a protective cover around it, and the ultraviolet light source switch is arranged outside the shell, and it passes through the shell to be connected to the ultraviolet light source.
[0019] Compared with the prior art, the present invention has the following beneficial effects:
[0020] The invention provides a method for preparing an aromatic hydrocarbon molecular imprinting fluorescent sensor. In the method for preparing the aromatic hydrocarbon molecular imprinting fluorescent sensor, carbon quantum dots are used as carriers, the carbon quantum dots are embedded in an aromatic hydrocarbon molecular polymer (aromatic hydrocarbon template molecules are removed), and then the aromatic hydrocarbon molecular imprinting fluorescent sensor is made into a fluorescent probe structure. The fluorescence spectrum thereof is measured by using a fluorescence detection device. When the excitation slit and the emission slit are 10 nm and 30 nm respectively, the obtained fluorescent sensor has excellent fluorescence characteristics. Meanwhile, the aromatic hydrocarbon molecular imprinting fluorescent sensor also has the advantage of being reusable. After the fluorescence intensity of the mixture is measured after adding solutions containing aromatic hydrocarbon molecules of different concentrations using the fluorescent sensor, the fluorescent sensor can be eluted with an eluent until an ultraviolet absorption spectrometer detects that the eluted solution does not contain an ultraviolet absorption peak of aromatic hydrocarbon molecules. The cleanly eluted fluorescent sensor can be reused after being tested to meet the standards. The aromatic hydrocarbon molecular imprinting fluorescent sensor prepared by the method can specifically identify aromatic hydrocarbon molecules, and the content of aromatic hydrocarbon molecules in the environment can be detected in real time by measuring the fluorescence intensity using a fluorescence spectrophotometer.
[0021] The present invention also provides a fluorescence intensity detection device for the aromatic hydrocarbon molecular imprinting fluorescence sensor. The detection device has a simple structure, low detection cost and high accuracy. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0023] Figure 1 : The scatter plot of Example 1 in Table 1 is drawn with the natural logarithm of the aromatic hydrocarbon molecule concentration lnC as the abscissa and F1 / F2 as the ordinate
[0024] Figure 2 : The scatter plot of Example 2 in Table 1 is drawn with the natural logarithm of the aromatic hydrocarbon molecule concentration lnC as the abscissa and F1 / F2 as the ordinate
[0025] Figure 3 : The scatter plot of Example 3 in Table 1 is drawn with the natural logarithm of the aromatic hydrocarbon molecule concentration lnC as the abscissa and F1 / F2 as the ordinate
[0026] Figure 4 : The scatter plot of Example 4 in Table 1 is drawn with the natural logarithm of the aromatic hydrocarbon molecule concentration lnC as the abscissa and F1 / F2 as the ordinate
[0027] Figure 5 :Schematic diagram of the rapid detection principle of aromatic hydrocarbon molecular imprinting fluorescence sensor
[0028] Figure 6 :Schematic diagram of the structure of the fluorescence intensity detection device
[0030] Explanation of reference numerals: 1 UV light source switch; 2 UV light source; 3 protective cover; 4 first focusing lens; 5 interference filter; 6 dust-proof glass; 7 sample storage pool; 8 UV light absorption filter; 9 second focusing lens; 10 photodiode; 11 light-shielding box; 12 housing; 13 first cavity; 14 second cavity DETAILED DESCRIPTION
[0031] The following examples are provided for a better understanding of the present invention, but are not intended to limit the best mode of implementation, nor to limit the content and protection scope of the present invention. Any product identical or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior arts shall fall within the protection scope of the present invention.
[0032] If no specific experimental steps or conditions are specified in the examples, the conventional experimental steps or conditions described in the literature in the field can be used. If no manufacturer is specified for the reagents or instruments used, they are all conventional reagent products that can be obtained commercially.
[0033] Example 1: A method for preparing an aromatic hydrocarbon molecular imprinting fluorescence sensor, comprising the following steps:
[0034] Preparation of S1 mixed solution: 1 mmol of aromatic hydrocarbon molecules and 3.9 mmol of α-methylacrylic acid are added to 10 ml of acetonitrile, and then ultrasonic vibration treatment is performed to fully dissolve them to obtain a mixed solution;
[0035] S2 Preparation of aromatic molecular polymer: Add 5 mmol of ethylene glycol dimethacrylate, 0.18 g of azobisisobutyronitrile and 0.046 g of carbon quantum dots (CDs) to the mixed solution obtained in step S1, introduce nitrogen to remove oxygen, then heat in a water bath at 50°C, fully react for 24 hours, and then vacuum dry to obtain an aromatic molecular polymer (MIP);
[0036] S3 Preparation of aromatic molecular imprinting fluorescence sensor: The aromatic molecular polymer obtained in step 2 is eluted with methanol acetic acid eluent, wherein the volume fraction of acetic acid is 5%, until the ultraviolet absorption spectrometer detects that there is no ultraviolet absorption peak of aromatic molecules in the eluent, thereby obtaining an aromatic molecular imprinting fluorescence sensor.
[0037] Example 2: A method for preparing an aromatic hydrocarbon molecular imprinting fluorescence sensor, comprising the following steps:
[0038] Preparation of S1 mixed solution: 1 mmol of aromatic hydrocarbon molecules and 4 mmol of α-methylacrylic acid are added to 20 ml of acetonitrile, and then subjected to ultrasonic vibration treatment to fully dissolve them to obtain a mixed solution;
[0039] S2 Preparation of aromatic molecular polymer: Add 10 mmol of ethylene glycol dimethacrylate, 0.2 g of azobisisobutyronitrile and 0.05 g of carbon quantum dots (CDs) to the mixed solution obtained in step S1, introduce nitrogen to remove oxygen, then heat in a water bath at 60°C, fully react for 24 hours, and then vacuum dry to obtain an aromatic molecular polymer (MIP);
[0040] S3 Preparation of aromatic molecular imprinting fluorescence sensor: The aromatic molecular polymer obtained in step 2 is eluted with methanol acetic acid eluent, wherein the volume fraction of acetic acid is 10%, until the ultraviolet absorption spectrometer detects that there is no ultraviolet absorption peak of aromatic molecules in the eluent, thereby obtaining an aromatic molecular imprinting fluorescence sensor.
[0041] Example 3: A method for preparing an aromatic hydrocarbon molecular imprinting fluorescence sensor, comprising the following steps:
[0042] Preparation of S1 mixed solution: 1 mmol of aromatic hydrocarbon molecules and 4.1 mmol of α-methylacrylic acid are added to 30 ml of acetonitrile, and then ultrasonic vibration treatment is performed to fully dissolve them to obtain a mixed solution;
[0043] S2 Preparation of aromatic molecular polymer: 15 mmol of ethylene glycol dimethacrylate, 0.23 g of azobisisobutyronitrile and 0.056 g of carbon quantum dots (CDs) were added to the mixed solution obtained in step S1, nitrogen was introduced to remove oxygen, and then heated in a water bath at 70°C, fully reacted for 24 hours, and then vacuum dried to obtain an aromatic molecular polymer (MIP);
[0044] S3 Preparation of aromatic molecular imprinting fluorescence sensor: The aromatic molecular polymer obtained in step 2 is eluted with methanol acetic acid eluent, wherein the volume fraction of acetic acid is 15%, until the ultraviolet absorption spectrometer detects that there is no ultraviolet absorption peak of aromatic molecules in the eluent, thereby obtaining an aromatic molecular imprinting fluorescence sensor.
[0045] Example 4: A method for preparing an aromatic hydrocarbon molecular imprinting fluorescence sensor, comprising the following steps:
[0046] Preparation of S1 mixed solution: 1 mmol of aromatic hydrocarbon molecules and 4 mmol of α-methylacrylic acid are added to 20 ml of acetonitrile, and then subjected to ultrasonic vibration treatment to fully dissolve them to obtain a mixed solution;
[0047] S2 Preparation of aromatic molecular polymer: Add 10 mmol of ethylene glycol dimethacrylate and 0.2 g of azobisisobutyronitrile to the mixed solution obtained in step S1, introduce nitrogen to remove oxygen, then heat in a water bath at 60°C, fully react for 24 hours, and then vacuum dry to obtain aromatic molecular polymer (MIP);
[0048] S3 Preparation of aromatic molecular imprinting fluorescence sensor: The aromatic molecular polymer obtained in step 2 is eluted with methanol acetic acid eluent, wherein the volume fraction of acetic acid is 10%, until the ultraviolet absorption spectrometer detects that there is no ultraviolet absorption peak of aromatic molecules in the eluent, thereby obtaining an aromatic molecular imprinting fluorescence sensor.
[0049] Standard solutions containing aromatic molecules at different concentrations were prepared, and then added to the aromatic molecular imprinting fluorescent sensors prepared in Examples 1 to 4 to obtain a mixture. The mixture was then fully oscillated for 30 minutes under light-proof conditions to allow the aromatic molecules to be fully adsorbed on the surface of the fluorescent probe. Finally, the fluorescence intensity of each mixed solution was measured using a fluorescence intensity detection device, with the excitation and emission slits being 10 nm and 30 nm, respectively, and the fluorescence intensity at 620 nm was recorded. The recorded data are shown in the following table:
[0050] Table 1
[0051]
[0052]
[0053] Note: lnC is the natural logarithm of the concentration of aromatic molecules; F1 is the initial fluorescence intensity of the fluorescent probe; F2 is the fluorescence intensity after adding solutions containing aromatic molecules of different concentrations
[0054] Figure 1 , 2 , 3, and 4 are scatter plots corresponding to Examples 1 to 4 in Table 1. Figures 1 to 4The scatter plots are all drawn with the natural logarithm of the aromatic hydrocarbon molecule concentration, lnC, as the horizontal axis and F1 / F2 as the vertical axis. Figures 1 to 4 It can be seen intuitively that the ratio of fluorescence intensity F1 / F2 shows a strong linear correlation with the concentration of aromatic molecules, which means that the concentration of aromatic molecules can be determined by fluorescence quenching. Among them, the detection effect of the aromatic molecular imprinting fluorescence sensor prepared in Example 2 is the best because Figure 1 , 2 , 3, 4 scatter plot R 2 It can be seen from the value that Figure 2 R 2 The value is closest to 1, indicating that its discreteness is the smallest and the linear relationship is the best.
[0055] Comparative Example 1: The aromatic hydrocarbon molecular imprinted fluorescent sensor prepared in Examples 1 to 4 was washed with an eluent after use until the ultraviolet absorption spectrometer detected that the eluent did not contain the ultraviolet absorption peak of aromatic hydrocarbon molecules, and then the fluorescence intensity of the eluted aromatic hydrocarbon molecular imprinted fluorescent sensor was detected to obtain F3, and F3 was compared with the initial fluorescence intensity F1 to obtain the following table data:
[0056] Table 2
[0057] Example 1 2 3 4 <![CDATA[F3 / F1]]> 0.98 1.0 0.97 0.99
[0058] It can be seen from Table 2 that the fluorescence intensity of the aromatic hydrocarbon molecular imprinted fluorescence sensor after elution with the eluent is almost the same as the initial fluorescence intensity. Therefore, the aromatic hydrocarbon molecular imprinted fluorescence sensor provided by the present invention also has the advantage of repeatability.
[0059] Embodiment 5: A fluorescence intensity detection device of an aromatic hydrocarbon molecular imprinting fluorescence sensor, wherein a shell 12 is arranged above a sample storage pool 7, a dustproof glass 6 is arranged at one end of the shell 12 close to the sample storage pool 7, a first cavity 13 is arranged directly above the dustproof glass 6, and an ultraviolet light absorption filter 8, a second focusing lens 9, a photodiode 10, and a light-proof box 11 are arranged in the first cavity 13; wherein the ultraviolet light absorption filter 8 is arranged at one end of the first cavity 13 close to the dustproof glass 6, a photodiode 10 is arranged at one end of the first cavity 13 away from the dustproof glass 6, a light-proof box cover 12 is arranged above the light-proof box 11, the second focusing lens 9 is arranged between the photodiode 10 and the ultraviolet light absorption filter 8, and the light-proof box 11 is arranged outside the end of the shell 12 away from the sample storage pool 7. A second cavity 14 is provided on the oblique side of the dustproof glass 6, and an interference filter 5, a first focusing lens 4 and an ultraviolet light source 2 are provided in the second cavity 14; wherein the interference filter 5 is provided at one end of the second cavity 14 close to the dustproof glass 6, the ultraviolet light source 2 is provided at one end of the second cavity 14 away from the dustproof glass 6, and the first focusing lens 4 is provided between the interference filter 5 and the ultraviolet light source 2; the ultraviolet light source 2 is wrapped with a protective cover 3 around it, and the ultraviolet light source switch 1 is provided outside the housing 12 and connected to the ultraviolet light source 2 through the housing 12. The sample storage pool 7 is provided with a groove at a position directly below the dustproof glass 6 for storing the sample to be tested.
[0060] The device uses a light source switch 1 to control the ultraviolet light source 2 to generate an ultraviolet light source of a specific wavelength, and then converges the light source to a specified position through a first focusing lens 4. The interference filter 5 and a dustproof glass 6 are also included on its path, and finally irradiate the sample solution in the sample pool 7. Under the stimulation of the ultraviolet light source 2, the excited fluorescence of the sample solution passes through the ultraviolet absorption filter 8, and then is transmitted to the photodiode 10 through the second focusing lens 9 and converted into an electrical signal, and then the obtained electrical signal output value is displayed on the display screen. The ultraviolet light source 2 irradiates the sample solution at an angle of 45°, and the ultraviolet absorption filter 8 can absorb the ultraviolet light source, which reduces the interference of the ultraviolet light source on the fluorescence intensity detection and improves the accuracy of fluorescence detection.
[0061] In summary, the preparation method of an aromatic hydrocarbon molecular imprinting fluorescence sensor and a fluorescence intensity detection device provided by the present invention can realize efficient and rapid detection of aromatic hydrocarbon molecules at room temperature; the aromatic hydrocarbon molecular imprinting fluorescence sensor can also be used repeatedly, so that the detection cost is low. At the same time, the fluorescence detection device has a simple structure, fast detection and high accuracy.
[0062] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived from these are still within the scope of protection of the invention.
Claims
1. A method for preparing an aromatic hydrocarbon molecular imprinted fluorescence sensor, characterized in that: The following steps are involved: Preparation of S1 mixed solution: acetonitrile is added into the reactor, and then aromatic molecules and α-methylacrylic acid are added into the acetonitrile, and after the addition is completed, ultrasonic vibration treatment is performed to obtain a mixed solution; S2 Preparation of aromatic molecular polymer: Add oxalic acid dimethyl acrylate, azobisisobutyronitrile and carbon quantum dots to the mixed solution obtained in step S1, replace oxygen with nitrogen after the addition is completed, heat in a water bath for reaction, and after the reaction is completed, vacuum dry the reaction solution to obtain an aromatic molecular polymer; S3 Preparation of aromatic hydrocarbon molecular imprinting fluorescence sensor: The aromatic hydrocarbon molecular polymer obtained in step S2 is eluted with methanol acetic acid eluent until the ultraviolet absorption spectrometer detects that there is no ultraviolet absorption peak of aromatic hydrocarbon molecules in the eluent, thereby obtaining an aromatic hydrocarbon molecular imprinting fluorescence sensor.
2. The method for preparing an aromatic hydrocarbon molecular imprinted fluorescence sensor according to claim 1, characterized in that: In the S1 mixed solution preparation step, the molar ratio of the aromatic hydrocarbon molecules to α-methyl acrylic acid is 1:(3.9-4.1); the molar ratio of the aromatic hydrocarbon molecules to acetonitrile is 1:(19-58).
3. The method for preparing an aromatic hydrocarbon molecular imprinted fluorescence sensor according to claim 1, characterized in that: In the S2 aromatic hydrocarbon molecule polymer preparation step, the molar ratio of oxalic acid dimethacrylate to aromatic hydrocarbon molecules is (5-15):
1.
4. The method for preparing an aromatic hydrocarbon molecular imprinted fluorescent sensor according to claim 1, characterized in that: In the S2 aromatic molecular polymer preparation step, the molar ratio of the azobisisobutyronitrile to the aromatic molecules is 1:(0.7-0.9); the mass ratio of the carbon quantum dots to the azobisisobutyronitrile is 1:(3.9-4.1).
5. The method for preparing an aromatic hydrocarbon molecular imprinted fluorescent sensor according to claim 1, characterized in that: In the S2 aromatic molecular polymer preparation step, the water bath heating temperature is 50-70°C.
6. The method for preparing an aromatic hydrocarbon molecular imprinted fluorescence sensor according to claim 1, characterized in that: In the preparation step of the aromatic hydrocarbon molecular imprinting fluorescent sensor, the volume fraction of the acetic acid is 5-15%.
7. A fluorescence intensity detection device for an aromatic hydrocarbon molecular imprinting fluorescence sensor, characterized in that: A shell (12) is arranged above a sample storage pool (7); a dustproof glass (6) is arranged inside one end of the shell (12) close to the sample storage pool (7); a first cavity (13) is arranged directly above the dustproof glass (6); an ultraviolet light absorption filter (8), a second focusing lens (9), a photodiode (10), and a light-shielding box (11) are arranged in sequence from bottom to top at one end of the first cavity (13) close to the bottom of the shell (12); a second cavity (14) is arranged in an oblique direction of the dustproof glass (6); an interference filter (5), a first focusing lens (4), and an ultraviolet light source are arranged in sequence from bottom to top at one end of the second cavity (14) close to the bottom of the shell (12).
8. The fluorescence intensity detection device of an aromatic hydrocarbon molecular imprinting fluorescence sensor according to claim 7, characterized in that: The sample storage pool (7) is provided with a groove at a position directly below the dustproof glass (6) for storing the sample to be tested.
9. The fluorescence intensity detection device of an aromatic hydrocarbon molecular imprinting fluorescence sensor according to claim 7, characterized in that: The ultraviolet light absorption filter (8) is arranged at one end of the first cavity (13) close to the dustproof glass (7), a light-shielding box (11) is arranged outside the first cavity (13) at one end away from the dustproof glass (7), the photodiode (10) is arranged inside the first cavity (13) at one end away from the dustproof glass (7), and the second focusing lens (9) is arranged between the photodiode (10) and the ultraviolet light absorption filter (8).
10. The fluorescence intensity detection device of an aromatic hydrocarbon molecular imprinting fluorescence sensor according to claim 7, characterized in that: The interference filter (5) is arranged at one end of the second cavity (14) close to the dustproof glass (6), the ultraviolet light source (2) is arranged at one end of the second cavity (14) away from the dustproof glass (6), and the first focusing lens (4) is arranged between the interference filter (5) and the ultraviolet light source (2); the ultraviolet light source (2) is wrapped with a protective cover (3) around it, and the ultraviolet light source switch (1) is arranged outside the housing (12) and passes through the housing (12) to be connected to the ultraviolet light source (2).