Automatic detection biochemical analyzer

Abstract

The invention relates to an automatic detection biochemical analyzer. The automatic detection biochemical analyzer comprises a multi-way valve 1, a multi-way valve 2, a peristaltic pump I, a peristaltic pump II, a peristaltic pump III, a peristaltic pump IV, a sampling valve and other connecting pipelines, the multi-way valve 1 is mounted at a feeding position, and the multi-way valve 2 is mounted at a discharging position; the first peristaltic pump, the second peristaltic pump, the third peristaltic pump and the fourth peristaltic pump are power transmission pumps for sampling or other materials, and a switching path is carried out by matching a cam mechanism in the center with a spring. The detection pool is subjected to injection molding by adopting high-transmittance plastic according to the principle of a conventional biochemical analyzer, and is embedded in the circulating pipeline; except that the sampling valve needs to be installed in a sample pool, other components are connected through pipelines and then integrally assembled through a plastic plate with a fixed size, and then sterilization treatment is conducted; during use, each interface is aseptically connected with a sampling valve, a substrate and a waste liquid bag; a pump, a sampling valve, a multi-way valve and a cam mechanism are logically controlled by a microcomputer system, system constants are calibrated by a standard sample, and the concentration of a sample is calculated through the system constants calibrated by the standard sample.

Description

Technical Field

[0001] The present application relates to the field of biochemical detection, and more particularly to an automatic biochemical detection instrument. Background Art

[0002] In the field of biochemical detection, through specific biochemical reactions, substances with maximum light absorption at specific wavelengths are generated, thereby reflecting the concentration of the object being measured, that is, the absorption light ΔA=KCL, K is the molar absorption coefficient, C is the concentration of the absorbing substance, and L is the layer thickness of the liquid in the absorption light path. The K value is obtained by measuring substances with known concentrations, and then the unknown concentration of substances is estimated. By concentrating or diluting the concentration of the object being measured to fall within the detection range, the accurate concentration of the unknown substance is obtained by the absorption light intensity ΔA. This method requires manual sampling, dilution, reaction, injection, detection, reading, and calculation before the sample enters the detection instrument; the process is prone to human errors and the operation is cumbersome.

[0003] Aiming at the above key technologies, the present invention intends to solve the defects in traditional biochemical detection. Summary of the invention

[0004] In order to solve the tedious operations of manual sampling, dilution, reaction, injection, detection, reading and calculation, the present application provides an automated biochemical detector.

[0005] The present application provides an automated biochemical detector using the following technical solutions:

[0006] The present application replaces conventional manual sampling with automatic sampling by the system; manual mixing of reagent reactions is replaced with circulating mixing by a peristaltic pump, and the reading and calculation are automatically stored, calculated and recorded by the system.

[0007] Preferably, the automatic sampling described in the present application uses a peristaltic pump in combination with a multi-channel valve to quantitatively draw a certain volume of sample from the sample pipeline, and cooperates with fermentation filtration, automatic opening and closing of the sampling valve, and an isolation membrane to ensure that the sample is an undisturbed fermentation liquid or a stock solution with isolated large particles.

[0008] The sampling valve of the isolation membrane isolates the particulate matter in the sample, such as bacteria, cells, carriers and other background substances that affect the light absorption intensity, thereby improving the detection accuracy of the detector and, secondly, avoiding the deposition of particulate matter on the detector.

[0009] Preferably, an automated biochemical detector is equipped with a multi-channel valve; the multi-channel valve has one channel for substrate transmission, one channel for internal circulation, and one channel for sample entry into the system. Through parallel openings in the cylinder, the outer shell channels cooperate to stretch and the corresponding channels are opened.

[0010] Through the multi-channel valve, quantitative sampling flows through the sample from the sample book; switch the main circulation pipeline to the reaction cycle or add the substrate, cooperate with the peristaltic pump as the material transmission power, realize the automatic control of the substrate into the system, the automatic control of quantitative sampling, and the automatic control of reaction mixing. If some reactions require the addition of reagents in steps, a multi-channel valve is added in the middle of the main circulation pipeline accordingly. Preferably, an automated biochemical detector, which is powered by a peristaltic pump to transmit materials, is installed in the main circulation pipeline and the sampling pipeline respectively. If multiple reagents are required, a peristaltic pump can be added according to the number of reagents.

[0011] Preferably, an automated biochemical detector is provided with a measuring cell installed on the main circulation tube, the measuring cell is composed of a conventional absorbance detection module, and the absorbance value feedback value control system records and stores it.

[0012] A preferred automated biochemical detector, a circulation pipeline, and a detection module are installed in a temperature control box. The temperature control box provides corresponding environmental temperature control for a specific reaction.

[0013] In summary, this application includes the following technical effects:

[0014] An automated biochemical analyzer that can automatically detect the content of some biological process reagents in a sterile environment; the detection module can be quickly replaced, and the sample concentration system can automatically calculate; sampling is automated, dilution is automated, and timing is controlled; and system data can be exchanged with the detection target. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of an existing conventional biochemical analyzer.

[0016] Figure 2 This is a system diagram of the actual application of this application example.

[0017] Figure 3 This is the main circuit conduction state of the multi-channel valve in this application example.

[0018] Figure 4 This is the state of the main circulation path of the multi-channel valve sampling path being turned on in this application example.

[0019] Figure 5 This is the sampling valve structure of the sample system of the present application example.

[0020] Figure 6 This is a schematic diagram of the overall structure of the test run for this application.

[0021] Description of reference numerals:

[0022] Figure 2:201, sample system; 202, sampling valve; 203, multi-way valve; 204, peristaltic pump; 205, peristaltic pump; 206, peristaltic pump; 207, multi-way valve 2; 208, detection cell; 209, peristaltic pump; 210, substrate bottle.

[0023] Figure 3 : Multi-way valve; 301, valve core; 302, valve body; 303, spring.

[0024] Figure 4 : Multi-channel valve; 401, substrate interface; 402, valve core substrate and main line connection port; 403 valve core, sampling and main line connection port; 404, valve core main circulation pipeline interface 1; 405, valve core sample interface; 406, sample line direct discharge connection port; 407, valve core sample direct discharge interface; 408, valve core main circulation interface 2.

[0025] Figure 5 : Sampling valve; 501, isolation membrane; 502, sampling valve body; 503, sampling outlet.

[0026] Figure 6 : Biochemical detection module integration; 601, temperature control box; 602, peristaltic pump No. 1; 603, peristaltic pump No. 2; 604, first interface; 605, multi-channel valve 1; 606, second interface; 607, third interface; 608, peristaltic pump No. 3; 609, peristaltic pump No. 4; 610, fourth interface; 611, fifth interface; 612, multi-channel valve 2; 613, cam mechanism; 614, sixth interface. DETAILED DESCRIPTION

[0027] The following combination Figures 1 to 6 To explain in further detail:

[0028] Reference Figure 1 , Figure 1 The invention discloses an existing detection method of a biochemical analyzer, which includes manual sampling, sample dilution, substrate reaction, adding into a sample pool, detecting absorbance value, and calculating sample concentration after repeating once or multiple times with a standard sample.

[0029] This example discloses an automatic biochemical detector, referring to Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6, take out the particle-free sample from the target sample pool through the sampling valve and the isolation membrane at its front end, and fill the entire inner cavity of the sample pipeline through the peristaltic pump; the multi-channel valve simultaneously fills the entire main circulation pipeline through the peristaltic pumps on both sides. After the main circulation pipeline is filled, the channel switching of the multi-channel valve is used to quantitatively sample from the sample pipeline to the main circulation pipeline, the substrate and the target detection sample, and the peristaltic pumps on both sides of the main circulation pipeline are used to allow the sample and the reaction substrate to fully react, and the temperature control of the temperature control box is started during the reaction. After the reaction is completed, the absorbance is measured by the detection pool part, and then the concentration of the detection object is output by the microcomputer system according to the absorbance.

[0030] Reference Figure 3 , Figure 4 , Figure 5 , Figure 6 The multi-channel valve is installed in the main circulation pipeline. There are several channels. The present application adopts three preferred channels. The valve core (301) is connected to the substrate interface and the sampling interface to the main circulation pipeline under the action of the spring (303) and the cam mechanism (613). In specific operation, when the cam mechanism (613) is in a low position and the spring lifts the multi-channel valve 1 (605), the substrate and the circulation main channel are connected. The No. 1 peristaltic pump (602) and the No. 3 peristaltic pump (608) on both sides of the circulation main channel simultaneously pump the substrate into the system to fill the entire pipeline from the multi-channel valve. The waste discharge interface (611) of 2 (612) empties the air in the main circuit, fills it with substrate, and closes the peristaltic pump No. 1 (602) and the peristaltic pump No. 3 (608) on both sides of the main circuit; while filling the substrate, the peristaltic pump (205) on the sampling pipeline simultaneously draws the sample from the system from the pipeline and fills it into the sampling channel of the multi-channel valve core; when the substrate filling and the sampling channel are completed at the same time, the cam mechanism (613) turns to the high position, lifts the multi-channel valve core, and at the same time, the main circuit waste discharge channel is switched to conduct the main circuit circulation, completing the substrate filling and sample entry into the system.

[0031] Reference Figure 6 When the substrate filling is completed and the sample enters the system, the temperature control box (601) is started to control the temperature, and the rotation direction of the peristaltic pump motors on both sides of the main circulation pipeline is switched to the same, and the substrate and sample are circulated, and the reaction is kept warm. After the reaction is completed, the absorbance value of the current sample is measured by the detection (208). In this way, the sample is a sample with a known concentration of the standard. The detection system is input by the operator into the system. The system calculates the absorption coefficient and prepares for the measurement of samples of unknown concentration. According to ΔA=KCL, the K value is determined using a standard sample of known concentration. The system can set an initialization process, connect the standard sample in parallel at the sampling end, and the system automatically initializes whenever a sample needs to be measured, and calibrates the system K value with the standard sample.

[0032] Reference Figure 5Preferably, an automatic sampling valve is used for sampling. When the sample in the sample pool needs to be measured, the control system sends an instruction to open the sampling valve to open the sampling valve. After the material passes through the filter membrane (501), it flows out from the sampling outlet (503) and is sucked into the detection system by the peristaltic pump. Then, the system closes the sampling valve.

[0033] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. An automatic biochemical analyzer, characterized in that It includes a detection module that can detect samples in a sterile environment, a peristaltic pump No. 1 (602), a peristaltic pump No. 2 (603), a multi-channel valve 1 (605), a peristaltic pump No. 3 (608), a peristaltic pump No. 4 (609), a multi-channel valve 2 (612), and a cam mechanism (613), which are installed on a board, and hoses connect various accessories; a first interface (604), a second interface (606), a third interface (607), a fourth interface (610), a fifth interface (611), and a sixth interface (614); aseptic connection, a peristaltic pump head, a pipeline, a detection module, a multi-channel valve, and sterilization after the overall assembly is completed.

2. An automatic biochemical analyzer, characterized in that A multi-channel valve 1 (605) and a multi-channel valve 2 (612) are used for sampling, substrate, and emptying, and a cam mechanism (613) is used to switch the channels. The multi-channel valve is composed of a valve body (302), a valve core (301), and a spring (303). The valve core can switch the channels of the valve body according to the positioning.

3. An automatic biochemical analyzer, characterized in that The sampling valve (502) has a membrane (501) installed at the front end thereof for isolating particles. The opening and closing of the sampling valve (502) can be controlled pneumatically or electrically, and the valve has two material interfaces (503).

4. An automatic biochemical analyzer, characterized in that The detection pool (208) for detecting absorbance is embedded in the circulation pipeline and is made of highly transparent plastic or glass. It can be sterilized by radiation and ethylene oxide without any material change.

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