Gel composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of biomedical materials, and provides a gel composition as well as a preparation method and application thereof. The gel composition provided by the invention takes the combination of the recombinant mussel mucin, the Ectoine, the sodium hyaluronate and the carboxymethyl chitosan as main preparation raw materials, and the raw material components can play a very good synergistic effect, so that the gel composition not only has extremely good biocompatibility, safety and stability, but also can be used for preparing the gel composition. The skin damage symptom can be well relieved, skin moisturizing and wound healing after beauty treatment are promoted, damaged skin repairing is promoted, the skin barrier function can be improved, and the health condition of damaged skin can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedical materials, and more specifically, to a gel composition and a preparation method and application thereof. Background Art

[0002] Laser, photon, injection of drugs / materials and other methods or ways to make the skin more beautiful are increasingly widely used methods. However, these external force intervention methods cause tiny wounds or burns on the skin, which requires a very important link, namely postoperative repair. At present, various dressings are usually used for postoperative repair of the skin, among which gel is easy to use, widely applicable, and widely welcomed.

[0003] Human skin fibroblasts can synthesize and secrete extracellular matrices such as hyaluronic acid and collagen fibers. Hyaluronic acid has excellent water-locking function, which can enhance skin hydration, provide cells with an excellent external environment, and maintain skin moisture. Collagen fibers are the main structural proteins of the dermal extracellular matrix, which play a supporting and anti-tension role and make the skin full. However, when the skin is damaged, such as after surgery, the cellular microenvironment in the skin will deteriorate, hyaluronic acid, collagen, etc. will be damaged and reduced, and the skin barrier function will be reduced. To repair damaged skin after surgery, it is necessary to heal the wound, enhance the skin's barrier function, and improve the cell microenvironment. However, the current repair gel is not ideal in promoting wound healing, enhancing the skin's barrier function, and improving the cell microenvironment after surgery.

[0004] Therefore, it is very important and necessary for skin care and postoperative repair to provide a stable, safe and effective gel composition that helps to heal wounds after surgery, maintain or increase the level of extracellular matrix components, and repair damaged skin. Summary of the invention

[0005] The present invention aims to solve at least one of the technical problems existing in the above-mentioned prior art. To this end, the present invention provides a gel composition and a preparation method and application thereof. The gel composition provided by the present invention not only has the function of supplementing the extracellular matrix, but also can stimulate and promote the proliferation of fibroblasts, promote collagen formation, promote wound healing, repair damaged skin, reduce and improve the adverse state of skin or mucous membrane, and enhance the skin barrier function.

[0006] A first aspect of the present invention provides a gel composition.

[0007] Specifically, a gel composition includes the following preparation raw materials: recombinant mussel mucin, ectoine, sodium hyaluronate, and carboxymethyl chitosan.

[0008] The present invention utilizes a combination of recombinant mussel mucin, ectoine, sodium hyaluronate, and carboxymethyl chitosan to achieve synergistic effects. The gel composition has excellent biocompatibility, safety, and stability, can effectively alleviate skin damage symptoms, promote skin moisturizing and wound healing after damage (such as after cosmetic surgery), promote damaged skin repair, and improve skin health.

[0009] Preferably, the gel composition comprises the following raw materials in terms of weight percentage: 1%-8% recombinant mussel mucin, 0.3%-2% ectoine, 0.1%-0.6% sodium hyaluronate, and 0.03%-0.25% carboxymethyl chitosan.

[0010] Further preferably, the gel composition comprises the following raw materials in terms of weight percentage: 1%-6% recombinant mussel mucin, 0.5%-1.5% ectoine, 0.12%-0.5% sodium hyaluronate, and 0.04%-0.2% carboxymethyl chitosan.

[0011] Further preferably, the gel composition comprises the following raw materials in terms of weight percentage: 2%-5% recombinant mussel mucin, 0.6%-1% ectoine, 0.15%-0.5% sodium hyaluronate, and 0.04%-0.15% carboxymethyl chitosan.

[0012] Further preferably, the gel composition comprises the following raw materials in terms of weight percentage: 3%-5% recombinant mussel mucin, 0.8%-0.9% ectoine, 0.15%-0.3% sodium hyaluronate, and 0.04%-0.1% carboxymethyl chitosan.

[0013] More preferably, the gel composition comprises the following raw materials in terms of weight percentage: 4% recombinant mussel mucin, 0.8% ectoine, 0.15% sodium hyaluronate, and 0.06% carboxymethyl chitosan.

[0014] Preferably, the mass content of dopa group (DOPA group, 3,4-dihydroxyphenylalanine) in the recombinant mussel mucin is 0.3wt%-5wt%.

[0015] Further preferably, the mass content of dopa groups in the recombinant mussel mucin is 0.5wt%-4wt%.

[0016] Further preferably, the mass content of dopa groups in the recombinant mussel mucin is 0.5wt%-3wt%.

[0017] More preferably, the mass content of dopa groups in the recombinant mussel mucin is 0.8wt%-1.5wt%.

[0018] Preferably, the sodium hyaluronate is a combination of high molecular weight sodium hyaluronate, medium molecular weight sodium hyaluronate and low molecular weight sodium hyaluronate, and the mass ratio of high molecular weight, medium molecular weight and low molecular weight sodium hyaluronate is (0.3-1.5):(0.5-2):(0.5-2); the weight average molecular weight of the high molecular weight sodium hyaluronate is 1 million Daltons to 1.5 million Daltons, the weight average molecular weight of the medium molecular weight sodium hyaluronate is 150,000 Daltons to 450,000 Daltons, and the weight average molecular weight of the low molecular weight sodium hyaluronate is 10,000 Daltons to 50,000 Daltons. Compared with sodium hyaluronate of a single molecular weight, the combination of sodium hyaluronates of different molecular weights is more conducive to keeping the skin moisturized, promoting wound healing, and improving the health of the skin.

[0019] More preferably, the mass ratio of high molecular weight, medium molecular weight and low molecular weight sodium hyaluronate is (0.3-1):(0.5-1.5):(0.5-1.5).

[0020] More preferably, the mass ratio of high molecular weight, medium molecular weight and low molecular weight sodium hyaluronate is 1:1:1.

[0021] Preferably, the weight average molecular weight of the high molecular weight sodium hyaluronate is 1.1 million Daltons to 1.4 million Daltons, and / or the weight average molecular weight of the medium molecular weight sodium hyaluronate is 200,000 Daltons to 400,000 Daltons, and / or the weight average molecular weight of the low molecular weight sodium hyaluronate is 10,000 Daltons to 50,000 Daltons.

[0022] Further preferably, the weight average molecular weight of the high molecular weight sodium hyaluronate is 1.2 million Daltons to 1.3 million Daltons, the weight average molecular weight of the medium molecular weight sodium hyaluronate is 200,000 Daltons to 300,000 Daltons, and the weight average molecular weight of the low molecular weight sodium hyaluronate is 20,000 Daltons to 30,000 Daltons.

[0023] More preferably, the weight average molecular weight of the high molecular weight sodium hyaluronate is 1.2 million Daltons to 1.3 million Daltons, the weight average molecular weight of the medium molecular weight sodium hyaluronate is 250,000 Daltons to 300,000 Daltons, and the weight average molecular weight of the low molecular weight sodium hyaluronate is 20,000 Daltons to 30,000 Daltons.

[0024] Preferably, the pH value of the gel composition is 5.2-6.2. If the pH is too high, the gel composition is unstable, and if the pH is too low, the composition is not easy to form a gel. This pH range helps the gel composition to be suitable for use on the skin and has good stability.

[0025] More preferably, the pH value of the gel composition is 5.2-6.0.

[0026] More preferably, the pH value of the gel composition is 5.2-5.8.

[0027] More preferably, the pH value of the gel composition is 5.3, 5.4, 5.5 or 5.6.

[0028] Preferably, the raw materials for preparing the gel composition further include at least one of a pH regulator, an auxiliary agent, and water.

[0029] Preferably, the pH adjuster is at least one of sodium hydroxide, potassium hydroxide, sodium carbonate and potassium carbonate.

[0030] Preferably, the auxiliary agent is at least one of carbomer, p-hydroxyacetophenone, 1,2-hexanediol, 1,3-butylene glycol, and glycerol.

[0031] Preferably, the gel composition comprises the following adjuvants in percentage by weight: 0.4%-1% carbomer, 0.4%-1% p-hydroxyacetophenone, 0.4%-1% 1,2-hexanediol, 0-10% 1,3-butylene glycol, and 0-10% glycerol.

[0032] Further preferably, the gel composition comprises the following adjuvants in percentage by weight: 0.4%-0.8% carbomer, 0.4%-0.8% p-hydroxyacetophenone, 0.4%-0.8% 1,2-hexanediol, 1%-8% 1,3-butanediol, and 1%-8% glycerol.

[0033] Further preferably, the gel composition comprises the following adjuvants in percentage by weight: 0.5%-0.8% carbomer, 0.5%-0.8% p-hydroxyacetophenone, 0.5%-0.8% 1,2-hexanediol, 1%-6% 1,3-butanediol, and 3%-8% glycerol.

[0034] Further preferably, the gel composition comprises the following adjuvants in percentage by weight: 0.6%-0.8% carbomer, 0.6%-0.8% p-hydroxyacetophenone, 0.6%-0.8% 1,2-hexanediol, 3%-5% 1,3-butanediol, and 5%-8% glycerol.

[0035] More preferably, the gel composition comprises the following adjuvants in terms of weight percentage: 0.6% carbomer, 0.6% p-hydroxyacetophenone, 0.6% 1,2-hexanediol, 5% by mass of 1,3-butanediol, and 6% glycerol.

[0036] Carbomer can be used as a matrix and / or thickener, which is conducive to the uniform and stable presence of recombinant mussel mucin in the composition, so that the composition does not precipitate precipitates / insoluble matter. Parahydroxyacetophenone is conducive to enhancing the moisture retention of the gel composition, and is also conducive to the stability of the gel composition, making it difficult to deteriorate. 1,2-Hexanediol is conducive to enhancing the moisture retention of the gel composition, and is also conducive to the stability of the gel composition, making it difficult to deteriorate.

[0037] A second aspect of the present invention provides a method for preparing a gel composition.

[0038] A method for preparing a gel composition comprises the following steps:

[0039] Recombinant mussel mucin, sodium hyaluronate, carboxymethyl chitosan and ectoine are respectively prepared to obtain recombinant mussel mucin solution, sodium hyaluronate solution, carboxymethyl chitosan solution and ectoine solution, then the sodium hyaluronate solution, carboxymethyl chitosan solution and ectoine solution are mixed, and then the recombinant mussel mucin solution is added to obtain the gel composition.

[0040] Preferably, recombinant mussel mucin, sodium hyaluronate, carboxymethyl chitosan and ectoine are dissolved in water to prepare recombinant mussel mucin solution, sodium hyaluronate solution, carboxymethyl chitosan solution and ectoine solution respectively.

[0041] Preferably, after adding the recombinant mussel mucin solution, the pH value is adjusted to 5.2-6.2.

[0042] Preferably, the raw materials for preparing the gel composition include: water, glycerol, 1,3-butylene glycol, carbomer, p-hydroxyacetophenone, 1,2-hexanediol and a pH regulator. The preparation method of the gel composition specifically includes: first preparing a 1,2-hexanediol solution of p-hydroxyacetophenone, mixing water, glycerol and 1,3-butylene glycol, adding carbomer, a 1,2-hexanediol solution of p-hydroxyacetophenone, a sodium hyaluronate aqueous solution, a carboxymethyl chitosan aqueous solution and an ectoine aqueous solution in sequence, then adding a recombinant mussel mucin aqueous solution, mixing, defoaming, homogenizing, and then adding a pH regulator to adjust the pH to 5.2-6.2 to obtain a gel composition.

[0043] Further preferably, the preparation method of the gel composition specifically comprises: mixing water, glycerol and 1,3-butylene glycol under stirring, stirring at 250rpm-300rpm for 10min-30min, then adding carbomer, and standing until swelling is complete; then homogenizing at 1000rpm-5000rpm for 1min-10min, vacuuming and defoaming, adding 1,2-hexanediol solution of p-hydroxyacetophenone under stirring, and stirring at 250rpm-300rpm for 10min-30min to obtain a mixed solution; stirring the mixture The solution is mixed with a sodium hyaluronate aqueous solution, a carboxymethyl chitosan aqueous solution and an ectoine aqueous solution, and stirred at 250 rpm-300 rpm for 10 min-30 min; under stirring, the prepared recombinant mussel mucin aqueous solution is added, stirred at 250 rpm-300 rpm for 10 min-30 min, vacuumed for defoaming, and homogenized at 1000 rpm-5000 rpm for 1 min-10 min; then the pH is adjusted to 5.2-5.8 with a pH adjuster, and the mixture is stirred and mixed evenly; and filtered with a 200-mesh filter cloth to obtain a gel composition.

[0044] The preparation method of the invention is simple, controllable and easy to operate, and can prevent the components in the composition from being degraded, denatured or otherwise failing to meet quality requirements.

[0045] The third aspect of the present invention provides a use of the gel composition.

[0046] The invention discloses an application of a gel composition in preparing a skin preparation.

[0047] A fourth aspect of the present invention provides a skin preparation.

[0048] A skin preparation comprises the gel composition.

[0049] Preferably, the skin preparation is a skin repair preparation.

[0050] The skin preparation provided by the present invention is an external preparation used for applying to the surface of human skin, such as the surface of skin after cosmetic or medical treatment, the surface of skin with small wounds, or the surface of skin after sunburn, or the surface of skin after burns.

[0051] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0052] The gel composition provided by the present invention uses recombinant mussel mucin, ectoine, sodium hyaluronate, and carboxymethyl chitosan as main raw materials for preparation. The raw material components can play a good synergistic role, so that the gel composition not only has excellent biocompatibility, safety, and stability (can be stably placed at room temperature for at least 7 days, or even up to 15 days), but can also effectively alleviate the symptoms of skin damage, promote moisturizing and wound healing of damaged skin (such as after cosmetic surgery), promote the repair of damaged skin, can enhance the skin barrier function, and is beneficial to improving the health of damaged skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0053] Figure 1 The state diagram of the gel composition of Example 1 to Example 3 after being placed for 15 days;

[0054] Figure 2 The irritation test results of the normal control group, the gel composition of Example 1, and the La Mer Concentrated Repair Essence (fluorescence image);

[0055] Figure 3 This is a test result diagram of the repairing effect of the gel composition in Example 1 (fluorescence diagram);

[0056] Figure 4 This is the test result of the repairing effect of La Mer Concentrated Repair Essence (fluorescent image). DETAILED DESCRIPTION

[0057] In order to make the technical scheme of the present invention more clearly understood by those skilled in the art, the following embodiments are listed for illustration. It should be pointed out that the following embodiments do not limit the protection scope of the present invention.

[0058] Unless otherwise specified, the raw materials, reagents or devices used in the following examples can be obtained from conventional commercial sources or by existing known methods.

[0059] The raw materials used in the examples and comparative examples of the present invention are:

[0060] Recombinant mussel mucin: DOPA group mass content is 1wt% and 5wt% (DOPA group mass content = DOPA group mass / recombinant mussel mucin mass × 100%), sourced from Wuhu Interfil Biological Products Industry Research Institute Co., Ltd.

[0061] Ekedoin: from Zhuhai Bairui Pharmaceutical Technology Co., Ltd.;

[0062] Sodium hyaluronate with different weight-average molecular weights was sourced from Shandong Focus Freda Biological Co., Ltd.;

[0063] Carboxymethyl chitosan: from Qingdao Honghai Biotechnology Co., Ltd., with a weight average molecular weight of 50,000-60,000 Daltons and a carboxymethyl substitution degree of 93.1%;

[0064] 10% sodium hydroxide: a 10% sodium hydroxide aqueous solution by mass.

[0065] Example 1

[0066] A gel composition includes the raw materials shown in Table 1 below (the total amount of each raw material added is 100wt%):

[0067] Table 1 Preparation materials and contents of the gel composition of Example 1

[0068]

[0069] The preparation method of the above-mentioned gel composition comprises the following steps:

[0070] (1) first prepare phase A (sodium hyaluronate aqueous solution), phase C (1,2-hexanediol solution of p-hydroxyacetophenone), phase D (carboxymethyl chitosan aqueous solution), phase E1 (ectoine aqueous solution), and phase F (sodium hydroxide aqueous solution);

[0071] (2) Add the water in phase B to the container, start stirring, add glycerol and 1,3-butylene glycol, stir for 15 minutes (250rpm-300rpm), homogenize for 3 minutes (3000rpm), add Carbomer 980 in phase B, stop homogenizing, and let stand for 10 hours; after the standing is over, start homogenization, homogenize for 3 minutes (3000rpm), and vacuum to defoam; then, add phase C first while stirring, and stir for 15 minutes (250rpm-300rpm); Then add phase A, phase D and phase E1, and stir for 15 minutes (250rpm-300rpm); then add the prepared phase E2 (recombinant mussel mucin aqueous solution), and stir for 15 minutes (250rpm-300rpm); vacuum defoam and homogenize for 3 minutes (3000rpm), then add phase F to adjust the pH to 5.4-5.8, add water to the total amount (i.e. to 100%), stir evenly, and filter with a 200-mesh filter cloth to obtain a clear and transparent gel composition.

[0072] Example 2

[0073] A gel composition includes the raw materials shown in Table 2 below (the total amount of each raw material added is 100wt%):

[0074] Table 2 Preparation materials and contents of the gel composition of Example 2

[0075]

[0076] The preparation method of the above gel composition is the same as that of Example 1.

[0077] Example 3

[0078] A gel composition includes the raw materials shown in Table 3 below (the total amount of each raw material added is 100 wt%):

[0079] Table 3 Preparation materials and contents of the gel composition of Example 3

[0080]

[0081]

[0082] The preparation method of the above gel composition is the same as that of Example 1.

[0083] Example 4

[0084] A gel composition, which differs from Example 1 in that the recombinant mussel mucin with a dopa group content of 1 wt% is replaced by a recombinant mussel mucin with a dopa group content of 5 wt%.

[0085] Example 5

[0086] A gel composition, which differs from Example 1 in that the large, medium and small molecular weight sodium hyaluronates are all replaced with an equal amount of sodium hyaluronate with a weight average molecular weight of 1.21 million Daltons to obtain a gel composition. It was found that in the process of preparing the gel composition, the mixed system was relatively viscous and it took longer to mix evenly.

[0087] Comparative Example 1

[0088] A gel composition, which differs from Example 1 in that the amount of ectoine added is replaced with 0.1wt%, and the amounts of other ingredients except water remain unchanged, to obtain a gel composition (put into a small bottle, and visually observed to have good fluidity when tilted).

[0089] Comparative Example 2

[0090] A gel composition, which differs from Example 1 in that carboxymethyl chitosan is replaced with chitosan of equal weight. It is found that chitosan is difficult to dissolve and disperse evenly; after all materials are mixed, the system has insoluble matter and is difficult to disperse evenly; it is difficult to prepare the product and conduct subsequent tests.

[0091] Product effect testing

[0092] 1. Appearance, color and skin feel of the gel composition

[0093] 25 healthy adults, aged 25-45 years old, with an average age of 30 years old, were selected and randomly divided into 1-5 groups, with 5 people in each group. The left hand of each group tested one sample in Examples 1-5, and the right hand tested the sample in Comparative Example 1. The specific operation was: wash both hands and dry them, apply 0.3 mL of the test product on the back of the hands, collect skin feel evaluation after application, and collect skin feel evaluation again 1 hour after application (without washing in between).

[0094] Results: The gel compositions obtained in Examples 1-3, when applied to the back of the hand, felt moisturized and acceptable; the gel composition obtained in Example 4 (slightly yellowish in color), when applied to the back of the hand, felt moisturized and acceptable; the gel composition obtained in Example 5 was relatively viscous, and when applied to the back of the hand, it felt sticky and had a poor skin feel. The gel composition obtained in Comparative Example 1, when applied to the back of the hand, was slightly thinner, had relatively higher fluidity, felt moisturized and acceptable, but had a slightly lower moisturizing degree and a slightly shorter duration than the gel composition in Example 1 (the moisturizing degree was reduced after 1 hour of application).

[0095] The gel compositions obtained in Examples 1-3 were placed in a small transparent glass bottle, sealed, and placed at room temperature at 25°C. It was found that the gel composition obtained in Example 2 changed color significantly after 7 days and turned yellow, indicating deterioration and instability ( Figure 1 B); there was no significant change in the gel compositions of Examples 1 and 3 after 15 days (see Example 1 and 3 for the gel compositions, respectively). Figure 1 A. Figure 1 C).

[0096] 2. Effect of gel composition on the expression of inflammatory factors in human immortalized keratinocytes

[0097] Preparation of samples: Take 0.039 g of the gel composition prepared in Example 1, add water to 100 g, mix well, and prepare before use.

[0098] Normal control group: cells were not irradiated with UV light and contained no samples;

[0099] Model control group: cells were exposed to ultraviolet light (80mJ / cm 2 ), without samples.

[0100] Then refer to the "Cosmetic Soothing Efficacy Test-In Vitro Skin Keratinocyte Inflammatory Factor Test Method T / GDCQMA 005-2024" to design the test:

[0101] Cell strain: immortalized human keratinocytes; cell generation: at least 2 generations; cell number per group: 6×10 5 Pieces / hole;

[0102] Cells were seeded in 6-well plates (6×10 5 / well), incubated at 37 ° C, 5% CO2 for 24 hours; after the incubation, the culture medium was removed, D-Hanks was washed 1-2 times, and then the sample group and the model control group were exposed to ultraviolet light (80mJ / cm 2 ); after the modeling was completed, the normal control group (not subjected to UV irradiation) and the model control group were replaced with fresh culture medium, and the sample group was added with fresh culture medium containing the sample, and continued to be incubated at 37°C, 5% CO2 for 24 hours; total RNA of each experimental group was extracted, cDNA was synthesized, and q-PCR (quantitative PCR) was used to detect the gene expression of β-actin (β-actin) and the target gene; β-actin was used as the internal reference of gene expression, and the relative RNA expression of the target gene was calculated according to the following formula (1);

[0103] Relative RNA expression = 2 ΔΔC(t) Formula (1)

[0104] Wherein, ΔΔC(t)=ΔC(t) model control group-ΔC(t) sample group, ΔC(t)=target gene C(t)-β-actin C(t); wherein C(t) represents the cycle threshold.

[0105] Table 4 Test results of the effect of the gel composition of Example 1 on the expression of inflammatory factors in human immortalized keratinocytes

[0106]

[0107]

[0108] Among them, the p value in the table is compared with the model control group, and p<0.05 indicates a significant difference.

[0109] According to the results in the above table, the gel composition provided in Example 1 of the present invention has a good effect on reducing the expression of inflammatory factors in damaged cells for three different genes, namely, TNF-α gene, IL-1α gene, and IL-6 gene, which is beneficial to the repair of damaged cells and skin repair.

[0110] 4. Irritation test

[0111] Sample preparation method: Take 1g of the gel composition of Example 1 and add water to 100g to obtain the test sample, which is prepared immediately before use. Take 1g of La Mer concentrated repair essence purchased from the market and add water to 100g to obtain the control sample.

[0112] The zebrafish test method was used, using transgenic neutrophil green fluorescent zebrafish (MPX), fish age: 2 days after fertilization (2dpf); according to the laboratory standard feeding and breeding methods, in line with the requirements of the international AAALAC certification, the adult fish were raised and bred.

[0113] Randomly select zebrafish in a 6-well plate, with 15 in each well; according to the set concentration, the test sample is dissolved (or dispersed) in water and added to the sample, and a normal control group (without the test sample) is set up at the same time, the volume of each well is 3 mL, and three biological replicates are performed, and the cells are incubated in the dark at 28°C for 18 hours; 10 zebrafish are randomly selected from each experimental group and photographed under a fluorescence microscope, and the data are analyzed and collected using advanced image processing software, and the number of neutrophils in the skin of zebrafish (N) is analyzed, and the irritation of the sample is calculated according to the following formula (2) to determine whether the test product is mild and non-irritating;

[0114] Irritation (%) = [(sample group N) - (normal control group N)] / (normal control group N) Formula (2)

[0115] The results are shown in the table below and Figure 2 (normal control group, sample of composition of Example 1 and sample of La Mer concentrated repair essence lotion), wherein, Figure 2 The dotted line area is the quantitative area.

[0116] Table 5 Irritation test results

[0117] sample Sample concentration Irritant P-value Test results Example 1 1％ -10％ >0.05 Not significant La Mer Essence 1％ -7％ >0.05 Not significant

[0118] In the table, p<0.05 indicates a significant difference.

[0119] According to the results in the above table, the gel composition provided by the present invention is non-irritating and is milder than the commercially available product La Mer Concentrated Repair Essence.

[0120] 5. Repair efficacy test

[0121] Sample group preparation method: Take 1g of the gel composition of Example 1 and add water to 100g to obtain the test sample, which is prepared immediately before use. Take 1g of La Mer concentrated repair essence purchased from the market and add water to 100g to obtain the control sample.

[0122] The zebrafish test method was adopted, using transgenic neutrophil green fluorescent zebrafish (MPX), fish age: 3 days after fertilization (2dpf); according to the laboratory standard feeding and breeding methods, in line with the requirements of international AAALAC certification, the adult fish were raised and bred.

[0123] Randomly select zebrafish, and use a scalpel to remove the tail fin of the zebrafish to establish a zebrafish tail fin injury and inflammation model; transfer the model zebrafish to a 6-well plate, with 15 fish in each well; according to the set concentration, dissolve (or disperse) the sample to be tested in water and add it to the sample, and set up a normal control group (without the test sample) and a model control group (i.e., the injury and inflammation model), each well has a capacity of 3 mL, and three biological replicates are performed, and incubated at 28°C in the dark for 5 hours; 10 zebrafish are randomly selected from each experimental group and photographed under a fluorescence microscope, and the data are analyzed and collected using advanced image processing software, and the number of neutrophils in the zebrafish tail fin area (N) is analyzed, and the irritation of the sample is calculated according to the following formula (3) to determine whether the test product has a repair effect;

[0124] Repair effect (%) = [(model control group N) - (sample group N)] / [(model control group N) - (normal control group N)] * 100% Formula (3)

[0125] The results are shown in the table below and Figure 3 (Sample prepared in Example 1) and Figure 4 (Control sample prepared with La Mer Essence Lotion). Figure 3 and Figure 4 The dotted line area is the quantitative area.

[0126] Table 6 Repair efficacy test results

[0127] sample Sample concentration Repair effect P-value Test results Example 1 1％ 53％ <0.001 Significant La Mer Essence 1％ 38％ <0.05 Significant

[0128] In the table, p<0.05 indicates a significant difference.

[0129] According to the results in the above table, the sample provided in Example 1 of the present invention has a good repairing effect on damaged skin, can enhance the skin barrier function, and is beneficial to improving the health of damaged skin.

Claims

1. A gel composition, characterized in that: The preparation raw materials include: recombinant mussel mucin, ectoine, sodium hyaluronate and carboxymethyl chitosan.

2. The gel composition according to claim 1, characterized in that The gel composition comprises the following raw materials in terms of weight percentage: 1%-8% recombinant mussel mucin, 0.3%-2% ectoine, 0.1%-0.6% sodium hyaluronate, and 0.03%-0.25% carboxymethyl chitosan.

3. The gel composition according to claim 1, characterized in that The mass content of dopa groups in the recombinant mussel mucin is 0.3wt%-5wt%.

4. The gel composition according to claim 1, characterized in that The sodium hyaluronate is a combination of high molecular weight sodium hyaluronate, medium molecular weight sodium hyaluronate and low molecular weight sodium hyaluronate, and the mass ratio of high molecular weight, medium molecular weight and low molecular weight sodium hyaluronate is (0.3-1.5):(0.5-2):(0.5-2); the weight average molecular weight of the high molecular weight sodium hyaluronate is 1 million Daltons to 1.5 million Daltons, the weight average molecular weight of the medium molecular weight sodium hyaluronate is 150,000 Daltons to 450,000 Daltons, and the weight average molecular weight of the low molecular weight sodium hyaluronate is 10,000 Daltons to 50,000 Daltons.

5. The gel composition according to claim 1, characterized in that The raw materials for preparing the gel composition also include at least one of a pH regulator, an auxiliary agent, and water.

6. The gel composition according to claim 5, characterized in that The auxiliary agent is at least one of carbomer, p-hydroxyacetophenone, 1,2-hexanediol, 1,3-butylene glycol and glycerol.

7. The gel composition according to claim 6, characterized in that The gel composition comprises the following auxiliary agents in terms of weight percentage: 0.4%-1% of carbomer, 0.4%-1% of p-hydroxyacetophenone, 0.4%-1% of 1,2-hexanediol, 0-10% of 1,3-butylene glycol, and 0-10% of glycerol.

8. The method for preparing the gel composition according to any one of claims 1 to 7, characterized in that: The steps include: Recombinant mussel mucin, sodium hyaluronate, carboxymethyl chitosan and ectoine are respectively prepared to obtain recombinant mussel mucin solution, sodium hyaluronate solution, carboxymethyl chitosan solution and ectoine solution, then the sodium hyaluronate solution, carboxymethyl chitosan solution and ectoine solution are mixed, and then the recombinant mussel mucin solution is added to obtain the gel composition.

9. Use of the gel composition according to any one of claims 1 to 7 in the preparation of a skin preparation.

10. A skin preparation, characterized in that: The invention comprises the gel composition according to any one of claims 1 to 7.

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