Transgenic corn detection device

Abstract

The invention relates to the technical field of gene detection devices, in particular to a transgenic corn detection device which comprises a detection box body, a first cover body and a second cover body, the rear half part of the upper side of the detection box body is detachably connected with the first cover body, and the front half part of the upper side of the detection box body is detachably connected with the second cover body. According to the invention, through the arrangement of the detection box body, the first cover body, the second cover body, the storage assembly, the mounting assembly and the detection clamping plate, the operation steps and the judgment standard of the device are relatively simple, a conclusion can be intuitively drawn through naked eyes, the requirement on a user is relatively low, and the device is small, portable and convenient to use. The device can be used only by being matched with a mobile power supply, a handheld homogenizer and a pipettor, the instruments and the device can be installed in a suitcase together, field detection is achieved, reagents used by the device can be stored and transported at normal temperature, the reaction time is short, and detection can be rapidly completed.

Description

Technical Field

[0001] The present invention relates to the technical field of gene detection devices, in particular to a genetically modified corn detection device. Background Art

[0002] Real-time fluorescence quantitative PCR technology is a genetically modified plant detection technology. It is a nucleic acid level detection that not only has the advantages of high sensitivity and speed, but also can perform both qualitative and quantitative detection. Currently, genetically modified corn detection devices that apply real-time fluorescence quantitative PCR technology are often used to detect genetically modified corn.

[0003] Some existing genetically modified corn detection devices have some problems during use: first, the operation steps and judgment criteria of the existing genetically modified corn detection devices are relatively complicated, and the requirements for users are high; second, the existing genetically modified corn detection devices are large in size and lack portability. Therefore, a genetically modified corn detection device is proposed to address the above problems. Summary of the invention

[0004] The purpose of the present invention is to provide a genetically modified corn detection device to solve the problems that the existing genetically modified corn detection devices have complex operation steps and judgment criteria, high requirements for users, and large size and insufficient portability.

[0005] To achieve the above object, the present invention provides the following technical solutions: A genetically modified corn detection device comprises a detection box, a first cover and a second cover, the upper rear half of the detection box is detachably connected to the first cover, the upper front half of the detection box is detachably connected to the second cover, the inner rear half of the detection box is provided with a storage assembly, the inner front half of the detection box is provided with a mounting assembly, the upper side of the mounting assembly is provided with a detection card plate, and the detection card plate is located in the detection box; the detection box comprises a heat insulation box and a partition plate, the inner side of the heat insulation box is fixedly connected to the partition plate, and the partition plate divides the heat insulation box into a first bin body and a second bin body, the left and right sides of the first bin body are fixedly connected to the first lock buckle, the left and right sides of the second bin body are fixedly connected to the second lock buckle, the outer side of the second bin body is rotatably connected to a rotating handle, the inner side of the first bin body is fixedly connected to a group of positioning plates, the inner side of the second bin body is fixedly connected to a uniform heat plate, and the first cover comprises a first heat insulation cover and a limiting sleeve , two limit sleeves are fixedly connected to the lower side of the first heat insulation cover, and the left and right sides of the first heat insulation cover are fixedly connected with third lock buckles, the second cover body includes a second heat insulation cover and a controller, the upper front half of the second heat insulation cover is fixedly connected with the controller, the lower front half of the second heat insulation cover is fixedly connected with a temperature sensor, the mounting holes of the second heat insulation cover are fixedly connected with color sensors, and the upper sides of the color sensors are fixedly connected with indicator lights, a pair of symmetrically distributed power plugs are fixedly connected to the lower side of the second heat insulation cover, a limit frame is fixedly connected to the lower side of the second heat insulation cover, and the left and right sides of the second heat insulation cover are fixedly connected with fourth lock buckles, the storage assembly includes a positioning plate and a limit block, and the left and right halves of the positioning plate are fixedly connected with a group of limit blocks, a first centrifuge tube is arranged in the limit groove of a group of limit blocks located on the left, and a second centrifuge tube is arranged in the limit groove of a group of limit blocks located on the right.

[0006] Preferably, the installation assembly includes a uniform heat plate and a heater, the heater is fixedly connected in the fixing groove of the uniform heat plate, a pair of symmetrically distributed power sockets are fixedly connected to the upper side of the uniform heat plate, a limiting frame is fixedly connected to the upper side of the uniform heat plate, the detection card board includes a card board body and a detection test tube, the detection test tubes are fixedly connected in the installation slot holes of the card board body, two tin foil seals are adhered to the upper side of the card board body, and a protective plate is fixedly connected to the lower side of the card board body.

[0007] Preferably, a rotating seat is provided on both sides of the left and right sides of the second warehouse body, the first lock buckle matches the third lock buckle, the second lock buckle matches the fourth lock buckle, the second insulation cover is provided with sixteen evenly distributed installation holes, the sealing covers of the first centrifuge tube and the second centrifuge tube are both fitted with the first insulation cover, and the sealing covers of the first centrifuge tube and the second centrifuge tube are both located in the limiting sleeve.

[0008] Preferably, the inner side of the limit block is provided with a limit groove, the first centrifuge tube and the second centrifuge tube are both composed of a tube body and a sealing cover, the lower side of the uniform heating plate is provided with a fixing groove, the power sockets are matched with power plugs, and the limit frame is composed of four vertical axes with card slots.

[0009] Preferably, the card board body is provided with sixteen evenly distributed mounting slots, the upper side of the card board body is fitted with the limit frame, the upper side of the detection test tube and the upper side of the card board body are both located in the same horizontal plane, the lower side of the tin foil seal is pasted with the detection test tube, and the protective plate is located on the inner side of the limit frame.

[0010] Preferably, the first centrifuge tube contains a lysis solution for rapid extraction of plant genomes at room temperature, the lysis solution in the first centrifuge tube consists of .M guanidine hydrochloride, .M NaCl, mMTrisHCl and mM EDTA, the second centrifuge tube contains a reconstitution solution, the detection test tube contains a freeze-dried amplification reagent, the freeze-dried amplification reagent in the detection test tube consists of a primer set, buffer, BstDNA polymerase and a freeze-dried protective agent.

[0011] Compared with the prior art, the present invention has the following beneficial effects: In the present invention, by providing a detection box, a first cover, a second cover, a storage assembly, an installation assembly and a detection card board, the operation steps and judgment criteria of the device are relatively simple, and conclusions can be drawn intuitively by the naked eye, with low requirements for users, and the device is small and portable, and can be used with only a mobile power supply, a handheld homogenizer and a pipette. The above instruments can be packed in a suitcase together with the device to achieve detection in the field. The reagents used in the device can be stored and transported at room temperature, and the reaction time is short, so the detection can be completed quickly. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the internal structure of the present invention; Figure 3 This is a schematic diagram of the installation structure of the positioning plate of the present invention; Figure 4 It is a schematic diagram of the structure of the storage assembly of the present invention; Figure 5 This is a schematic diagram of the structure of the installation assembly of the present invention; Figure 6 It is a cross-sectional view of the installation assembly structure of the present invention; Figure 7 This is a schematic diagram of the structure of the detection card board of the present invention; Figure 8 This is a schematic diagram of the protective plate installation structure of the present invention; Fig. 9 This is a schematic diagram of the first cover structure of the present invention; Fig.10 It is a cross-sectional view of the first cover structure of the present invention; Fig.11 This is a schematic diagram of the second cover structure of the present invention; Fig.12 is a cross-sectional view of the second cover structure of the present invention; Fig.13 It is a top view of the main structure of the card board of the present invention; Fig.14 It is a top view of the second thermal insulation cover structure of the present invention.

[0013] In the figure: 1. detection box; 11. heat insulation box; 12. partition plate; 13. first bin; 14. second bin; 15. first lock; 16. second lock; 17. rotating handle; 2. first cover; 21. first heat insulation cover; 22. limit sleeve; 23. third lock; 3. second cover; 31. second heat insulation cover; 32. controller; 33. temperature sensor; 34. color sensor; 35. indicator light; 36. power plug; 37. limit frame; 38. fourth lock; 4. storage assembly; 41. positioning plate; 42. limit block; 43. first centrifuge tube; 44. second centrifuge tube; 5. installation assembly; 51. uniform heating plate; 52. heater; 53. power socket; 54. limit frame; 6. detection card board; 61. card board body; 62. detection test tube; 63. tin foil seal; 64. protective plate. DETAILED DESCRIPTION

[0014] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0015] In the description of the present invention, it is necessary to understand that the directions or positional relationships indicated by directional words such as "front, back, up, down, left, right", "lateral, vertical, perpendicular, horizontal" and "top, bottom" are usually based on the directions or positional relationships shown in the drawings. They are only for the convenience of describing the present invention and simplifying the description. Unless otherwise specified, these directional words do not indicate or imply that the devices or elements referred to must have a specific direction or be constructed and operated in a specific direction. Therefore, they cannot be understood as limiting the scope of protection of the present invention. The directional words "inside and outside" refer to the inside and outside relative to the contours of each component itself.

[0016] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. If not otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of the present invention.

[0017] See also Figure 1-14 , the present invention provides a technical solution: A genetically modified corn detection device comprises a detection box 1, a first cover 2 and a second cover 3, wherein the upper rear half of the detection box 1 is detachably connected to the first cover 2, the upper front half of the detection box 1 is detachably connected to the second cover 3, the inner rear half of the detection box 1 is provided with a storage component 4, the inner front half of the detection box 1 is provided with a mounting component 5, the upper side of the mounting component 5 is provided with a detection card board 6, and the detection card board 6 is located in the detection box 1; the detection box 1 comprises a heat insulation box 11 and a partition plate 12, the heat insulation box 11 A partition plate 12 is fixedly connected to the inner side of the heat insulation box 11, and the partition plate 12 divides the heat insulation box 11 into a first warehouse body 13 and a second warehouse body 14. The left and right sides of the first warehouse body 13 are fixedly connected with a first lock buckle 15, and the left and right sides of the second warehouse body 14 are fixedly connected with a second lock buckle 16. The outer side of the second warehouse body 14 is rotatably connected with a rotating handle 17. A group of positioning plates 41 are fixedly connected to the inner side of the first warehouse body 13, and a uniform heat plate 51 is fixedly connected to the inner side of the second warehouse body 14. The first cover body 2 includes a first heat insulation cover 21 and a limit sleeve 22. The lower side of the first insulation cover 21 is fixedly connected with two limit sleeves 22, and the left and right sides of the first insulation cover 21 are fixedly connected with third lock buckles 23. The second cover body 3 includes a second insulation cover 31 and a controller 32. The upper front half of the second insulation cover 31 is fixedly connected with the controller 32, and the lower front half of the second insulation cover 31 is fixedly connected with a temperature sensor 33. The installation holes of the second insulation cover 31 are fixedly connected with color sensors 34, and the upper side of the color sensors 34 is fixedly connected with indicator lights 35. A pair of symmetrically distributed power plugs 36 are fixedly connected to the lower side of the cover 31, a limiting frame 37 is fixedly connected to the lower side of the second insulation cover 31, and a fourth lock buckle 38 is fixedly connected to the left and right sides of the second insulation cover 31. The storage assembly 4 includes a positioning plate 41 and a limiting block 42. The left and right halves of the positioning plate 41 are fixedly connected to a group of limiting blocks 42. A first centrifuge tube 43 is provided in the limiting groove of the group of limiting blocks 42 on the left side, and a second centrifuge tube 44 is provided in the limiting groove of the group of limiting blocks 42 on the right side.

[0018] Furthermore, the installation component 5 includes a uniform heating plate 51 and a heater 52, the heater 52 is fixedly connected in the fixed groove of the uniform heating plate 51, a pair of symmetrically distributed power sockets 53 are fixedly connected to the upper side of the uniform heating plate 51, a limiting frame 54 is fixedly connected to the upper side of the uniform heating plate 51, the detection card board 6 includes a card board body 61 and a detection test tube 62, the detection test tube 62 is fixedly connected in the installation slot holes of the card board body 61, two tin foil seals 63 are adhered to the upper side of the card board body 61, and a protective plate 64 is fixedly connected to the lower side of the card board body 61. The detection card board 6 can be heated by the installation component 5, and the sample can be detected by the detection card board 6.

[0019] Furthermore, a rotating seat is provided on both sides of the left and right sides of the second warehouse body 14, the first lock buckle 15 matches the third lock buckle 23, the second lock buckle 16 matches the fourth lock buckle 38, the second insulation cover 31 is provided with sixteen evenly distributed installation holes, the sealing covers of the first centrifuge tube 43 and the second centrifuge tube 44 are both fitted with the first insulation cover 21, and the sealing covers of the first centrifuge tube 43 and the second centrifuge tube 44 are both located in the limiting sleeve 22, the result can be interpreted through the second cover body 3, and the lysate and the re-solution solution can be stored through the storage component 4.

[0020] Furthermore, the inner side of the limit block 42 is provided with a limit groove, the first centrifuge tube 43 and the second centrifuge tube 44 are both composed of a tube body and a sealing cover, a fixing groove is provided on the lower side of the uniform heating plate 51, the power socket 53 is matched with the power plug 36, and the limit frame 54 is composed of four vertical axes with card slots. The detection card plate 6 can be engaged through the installation component 5 so that the detection card plate 6 no longer moves.

[0021] Furthermore, the card board body 61 is provided with sixteen evenly distributed installation slots, the upper side of the card board body 61 is fitted with the limit frame 37, the upper side of the detection test tube 62 and the upper side of the card board body 61 are both located on the same horizontal plane, the lower side of the tin foil seal 63 is pasted with the detection test tube 62, and the protective plate 64 is located on the inner side of the limit frame 54, and the freeze-dried amplification reagent can be stored through the detection card board 6.

[0022] Furthermore, the first centrifuge tube 43 contains a lysis solution for rapid extraction of plant genomes at room temperature, the lysis solution in the first centrifuge tube 43 is composed of 0.1M guanidine hydrochloride, 0.5M NaCl, 50mM TrisHCl and 2mM EDTA, the second centrifuge tube 44 contains a reconstitution solution, the detection test tube 62 contains a freeze-dried amplification reagent, the freeze-dried amplification reagent in the detection test tube 62 is composed of a primer set, Buffer, BstDNA polymerase and a freeze-dried protective agent, and the freeze-dried amplification reagent in the detection card plate 6 can be used to specifically identify the sixteen gene loci of corn.

[0023] Workflow: The device needs to be used with lysis solution, rehydration solution, and freeze-dried amplification reagent. The lysis solution used by the device is composed of 0.1M guanidine hydrochloride, 0.5M NaCl, 50mM TrisHCl and 2mM EDTA. The rehydration solution used by the device is an existing reagent. The freeze-dried amplification reagent used by the device is composed of a primer set, Buffer, BstDNA polymerase and a freeze-dried protective agent. The primer set is composed of corn DBN9936 transformant, DBN9501 transformant, Ruifeng 125 transformant, MIR162 transformant, Bt11 transformant, GA21 transformant, ND207 transformant, MON810 transformant, TC1507 transformant, MIR604 transformant, 59122 transformant, MO The N89034 transformant, MON88017 transformant, NK603 transformant, and DP4114 transformant templates are designed and obtained, and have the advantage of high specificity. The buffer is composed of Tris-HCl, MgSO4, KCl, phenol red, dNTPs, Tween20, and betaine, and the lyophilization protective agent is composed of trehalose, dextran, and mannitol. The sixteen mounting holes of the second heat-insulating cover 31 and the sixteen mounting slots of the card body 61 correspond to fifteen transgenic gene sites of corn and one corn internal standard gene site. During the detection process, the sample can be used for detection after the transgenic genome is fully released in the lysate. Phenol red is a weakly alkaline pH indicator with a color change range of pH6.8 (yellow) to pH8.0 (red), if the sample contains the target fragment, LAMP amplification can be achieved. During the LAMP reaction, each time a base is extended, the DNA polymerase will release a hydrogen ion, thereby gradually reducing the pH value of the system, and the system is displayed in yellow or light yellow. If the sample does not contain the target fragment, LAMP amplification cannot be achieved, the pH of the system does not decrease, and the system is displayed in red. Therefore, it is possible to determine whether the sample contains the transgenic target based on the color change of the reaction solution. The mounting holes of the second insulation cover 31 are fixedly connected with the color sensor 34, and the mounting slots of the card body 61 are fixedly connected with the detection test tube 62 The color of the reaction solution can be detected by the color sensor 34. If the reaction solution in the test tube 62 turns red, the indicator light 35 turns red. If the reaction solution in the test tube 62 does not turn red, the indicator light 35 does not turn red. Before using the device, the device is moved to a designated position by rotating the handle 17 and the power is turned on. The controller 32 of the device is electrically connected to the temperature sensor 33, the color sensor 34, the indicator light 35, and the power plug 36 respectively. The heater 52 is electrically connected to the two power sockets 53. The two power plugs 36 and the two power sockets 53 can be plugged in. The power plug 36 and the power socket 53 After plugging in, the device can operate normally. The manual operation controller 32 can adjust the operating status of the temperature sensor 33, the color sensor 34, the indicator light 35, and the heater 52. The partition plate 12 divides the heat insulation box 11 into the first warehouse body 13 and the second warehouse body 14. The temperature sensor 33, the color sensor 34, and the heater 52 are all existing instruments, and the above are all prior arts. When using the device, first release the lock of the third lock buckle 23 and the first lock buckle 15, remove the first insulation cover 21 and the limit sleeve 22, and pull out the first centrifuge tube 43 and the second centrifuge tube 44 fixed by the positioning plate 41 and the limit block 42 upwards, and then the first centrifuge tube 43 and the second centrifuge tube 44 can be removed from the first Take out the first centrifuge tube 43 and the second centrifuge tube 44 from the bin 13 for use, then release the lock of the fourth lock 38 and the second lock 16, and then take out the card plate body 61 from the second bin 14 for use, then take out 5 mg corn kernel powder or 5*2 mm corn leaves, add them to the first centrifuge tube 43 containing 0.4 mL lysis solution, use a handheld homogenizer to fully homogenize and lyse for 30 seconds to release corn nucleic acid, then take 0.025 mL of the above corn nucleic acid, add it to the second centrifuge tube 44 containing 0.4 mL reconstitution solution, vortex and oscillate for 5 seconds to fully mix for use, then tear off the tin foil seal 63, take 0.025mL of the above-mentioned reconstituted solution is added into a plurality of detection test tubes 62 containing freeze-dried amplification reagents and mixed thoroughly. Then, the card board body 61 is placed in the second bin body 14, the protective plate 64 is engaged by the limit frame 54, and the second heat-insulating cover 31 is reinstalled on the heat-insulating box 11, so that the power plug 36 is plugged into the power socket 53, and the lower side of the limit frame 37 is abutted against the card board body 61, and the detection test tube 62 can be fixed by the card board body 61 and the protective plate 64, and the temperature sensor 33, the color sensor 34, the indicator light 35, and the heater 52 are started by the controller 32. The heater 52 is used for heating, and the temperature is detected by the temperature sensor 33. The controller 32 can control the operating state of the heater 52. The heat is dissipated by the uniform heating plate 51, so that a constant temperature is maintained in the second chamber 14 and the detection test tube 62 is evenly heated. The result can be judged after thirty minutes of constant temperature reaction. The user can choose to judge with the naked eye and directly take out the card board body 61 to observe the detection test tube 62. If the liquid in the detection test tube 62 turns yellow or orange, the result is positive and the sample contains genetically modified organisms. If the liquid in the detection tube 62 turns red, the result is negative and the sample does not contain genetically modified organisms. The user can also choose the device to automatically read. After the reaction is completed, if the indicator light 35 lights up, it is positive, and if the indicator light 35 does not light up, it is negative. The operation steps and judgment criteria of the device are relatively simple, and the conclusion can be drawn intuitively by the naked eye. The requirements for users are relatively low, and the device is small and portable. It only needs to be equipped with a mobile power supply, a handheld homogenizer and a pipette. The above instruments can be packed in a suitcase with the device to achieve field detection. The reagents used in the device can be stored and transported at room temperature, and the reaction time is short, so the detection can be completed quickly. .

[0024] This article uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only used to help understand the method and core ideas of the present invention. The above is only a preferred implementation of the present invention. It should be pointed out that due to the limitations of textual expression and the objective existence of infinite specific structures, ordinary technicians in this technical field can make several improvements, modifications or changes without departing from the principles of the present invention, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the inventive concept and technical solution to other occasions without improvement, should be regarded as the protection scope of the present invention.

Claims

1. A genetically modified corn detection device, comprising a detection box (1), a first cover (2) and a second cover (3), characterized in that: The upper rear half of the detection box (1) is detachably connected to a first cover (2), the upper front half of the detection box (1) is detachably connected to a second cover (3), the inner rear half of the detection box (1) is provided with a storage assembly (4), the inner front half of the detection box (1) is provided with a mounting assembly (5), the upper side of the mounting assembly (5) is provided with a detection card board (6), and the detection card board (6) is located in the detection box (1); the detection box (1) comprises a heat insulation box (11) and a partition plate (12), the inner side of the heat insulation box (11) is fixedly connected to the partition plate (12), and the partition plate ( 12) The heat-insulating box (11) is divided into a first bin body (13) and a second bin body (14), the first bin body (13) is fixedly connected to first lock buckles (15) on both left and right sides, the second bin body (14) is fixedly connected to second lock buckles (16) on both left and right sides, the second bin body (14) is rotatably connected to a rotatable handle (17) on the outer side of the second bin body (14), the first bin body (13) is fixedly connected to a group of positioning plates (41) on the inner side, the second bin body (14) is fixedly connected to a uniform heat plate (51) on the inner side, the first cover body (2) comprises a first heat-insulating cover (21) and a limiting sleeve (22), the first heat-insulating cover (21) The lower side of the first heat insulation cover (21) is fixedly connected to two limit sleeves (22), the left and right sides of the first heat insulation cover (21) are fixedly connected to third lock buckles (23), the second cover body (3) comprises a second heat insulation cover (31) and a controller (32), the upper front half of the second heat insulation cover (31) is fixedly connected to the controller (32), the lower front half of the second heat insulation cover (31) is fixedly connected to a temperature sensor (33), the mounting holes of the second heat insulation cover (31) are fixedly connected to color sensors (34), the upper side of the color sensors (34) are fixedly connected to indicator lights (35), and the second heat insulation cover (31) is fixedly connected to the upper side of the color sensors (34). ) is fixedly connected to the lower side of a pair of symmetrically distributed power plugs (36), the lower side of the second heat insulation cover (31) is fixedly connected to a limit frame (37), the left and right sides of the second heat insulation cover (31) are fixedly connected to fourth lock buckles (38), the storage assembly (4) comprises a positioning plate (41) and a limit block (42), the left half and the right half of the positioning plate (41) are fixedly connected to a group of limit blocks (42), the limit grooves of the group of limit blocks (42) on the left side are provided with a first centrifuge tube (43), and the limit grooves of the group of limit blocks (42) on the right side are provided with a second centrifuge tube (44).

2. A genetically modified corn detection device according to claim 1, characterized in that: The mounting assembly (5) comprises a uniform heating plate (51) and a heater (52), the heater (52) being fixedly connected in a fixing groove of the uniform heating plate (51), a pair of symmetrically distributed power supply sockets (53) being fixedly connected to the upper side of the uniform heating plate (51), a limit frame (54) being fixedly connected to the upper side of the uniform heating plate (51), the detection card (6) comprising a card body (61) and a detection test tube (62), the detection test tube (62) being fixedly connected in the mounting groove holes of the card body (61), two tin foil seals (63) being adhered to the upper side of the card body (61), and a protective plate (64) being fixedly connected to the lower side of the card body (61).

3. A genetically modified corn detection device according to claim 1, characterized in that: The left and right sides of the second bin body (14) are both provided with a rotating seat, the first locking buckle (15) is matched with the third locking buckle (23), the second locking buckle (16) is matched with the fourth locking buckle (38), the second heat-insulating cover (31) is provided with sixteen evenly distributed mounting holes, the sealing covers of the first centrifuge tube (43) and the second centrifuge tube (44) are both fitted with the first heat-insulating cover (21), and the sealing covers of the first centrifuge tube (43) and the second centrifuge tube (44) are both located in the limiting sleeve (22).

4. A transgenic corn detection device according to claim 2, characterized in that: The inner side of the limit block (42) is provided with a limit groove, the first centrifuge tube (43) and the second centrifuge tube (44) are both composed of a tube body and a sealing cover, the lower side of the uniform heating plate (51) is provided with a fixing groove, the power socket (53) is matched with the power plug (36), and the limit frame (54) is composed of four vertical axes provided with slots.

5. A genetically modified corn detection device according to claim 2, characterized in that: The card plate body (61) is provided with sixteen evenly distributed mounting slots, the upper side of the card plate body (61) is in contact with the limit frame (37), the upper side of the detection test tube (62) and the upper side of the card plate body (61) are both located on the same horizontal plane, the lower side of the tin foil seal (63) is attached to the detection test tube (62), and the protective plate (64) is located on the inner side of the limit frame (54).

6. A genetically modified corn detection device according to claim 2, characterized in that: The first centrifuge tube (43) contains a lysis solution for rapid extraction of plant genomes at room temperature, the lysis solution in the first centrifuge tube (43) is composed of 0.1M guanidine hydrochloride, 0.5M NaCl, 50mM TrisHCl and 2mM EDTA, the second centrifuge tube (44) contains a reconstitution solution, the detection test tube (62) contains a freeze-dried amplification reagent, the freeze-dried amplification reagent in the detection test tube (62) is composed of a primer set, a buffer, a BstDNA polymerase and a freeze-dried protective agent.

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