Slow-release bacteriostatic skin cleansing lotion containing nano-silver microcapsules as well as preparation method and application of skin cleansing lotion

Abstract

The invention relates to the technical field of cleansing lotion, and discloses a sustained-release bacteriostatic cleansing lotion containing nano-silver microcapsules. Comprising the following raw materials: potassium cocamidoglycinate, sodium lauroyl glutamate, cocamidopropyl betaine, cocoanut fatty acid diethanolamide, fatty acid methyl ester ethoxylate, tea saponin, COSE-Ag NPs microcapsules, glycerol, EDTA (Ethylene Diamine Tetraacetic Acid) disodium, citric acid, deionized water, methylparaben, oat beta-glucan, a honeysuckle flower extract and a Chinese soapberry fruit extracting solution. According to the invention, nano-silver (COSE) AgNPs is prepared from a cornus officinalis kernel extract, and a technology of coating the nano-silver with microcapsules is applied to the skin cleansing lotion. The invention finds that the nano-silver microcapsule has an obvious slow-release antibacterial effect when being applied to the skin cleansing lotion, the FMEE has good hard water decontamination capability, the micron-level foam is relatively fine, and the surface of human skin can be kept clean for a long time.

Description

Technical Field

[0001] The invention belongs to the technical field of preparing nano-silver from cornus officinalis kernel extract and applying it to skin cleansing lotion, and particularly relates to a skin cleansing lotion containing nano-silver microcapsules for sustained-release and antibacterial effects, a preparation method and uses thereof. Technical Background

[0002] Miners are eroded and polluted by coal dust, debris, oil stains, germs and other problems for a long time. In particular, germs will enter the skin through the pores of miners' sweat, causing skin diseases. Generally, the water in mining areas has high hardness and is rich in calcium and magnesium ions. Ordinary soap will form insoluble stearates in hard water, thus reducing its decontamination ability. Because the top three skin diseases caused by coal black are callus, tinea pedis and tinea manuum, which seriously affect the physical and mental health of miners, it is urgent to develop an antibacterial cleanser. Summary of the invention

[0003] The purpose of the present invention is to provide a skin cleanser containing nano-silver microcapsules for sustained-release and antibacterial effects, which comprises the following raw materials: potassium cocamide glycinate, sodium lauroyl glutamate, cocamidopropyl betaine, coconut oil fatty acid diethanolamide, fatty acid methyl ester ethoxylate, tea saponin, COSE@AgNPs microcapsules, glycerin, disodium EDTA, citric acid, deionized water, methylparaben, oat β-glucan, honeysuckle extract, and soapberry extract.

[0004] Nanosilver is a new antibacterial product based on nanomaterial technology, namely, COSE@AgNPs, which has more stable physical and chemical properties than silver ions. As an antibacterial agent, nanosilver belongs to the non-antibiotic class and can overcome bacterial resistance. The mechanism of action of nanosilver is that nanosilver particles directly enter the bacteria and combine with the sulfhydryl group (-SH) of the oxygen metabolism enzyme, suffocating the bacteria to death, thereby exerting a bactericidal effect.

[0005] Preferably, the proportions of each raw material component are as follows: 4-8 mL of potassium cocamide glycinate, 2-4 mL of sodium lauroyl glutamate, 8-12 mL of cocamidopropyl betaine, 1-2 mL of coconut oil fatty acid diethanolamide, 0.5-1 mL of fatty acid methyl ester ethoxylate, 0.8-1.2 g of tea saponin, 0.8-1.2 g of COSE@AgNPs microcapsules, 4-6 g of glycerol, 0.08-0.12 g of disodium EDTA, 1-2 mL of citric acid, 40-80 mL of deionized water, 0.2 g of methyl paraben, 1.4-1.8 g of oat β-glucan, 0.3-0.7 g of honeysuckle extract, and 0.3-0.7 mL of soapberry extract.

[0006] Preferably, the preferred proportions of each raw material component are as follows: 6 mL of potassium cocamide glycinate, 3 mL of sodium lauroyl glutamate, 10 mL of cocamidopropyl betaine, 1.5 mL of coconut oil fatty acid diethanolamide, 1 mL of fatty acid methyl ester ethoxylate, 1 g of tea saponin, 1 g of COSE@AgNPs microcapsules, 5 g of glycerol, 0.1 g of disodium EDTA, 1.5 mL of citric acid, 50 mL of deionized water, 0.2 g of methyl paraben, 1.6 g of oat β-glucan, 0.5 g of honeysuckle extract, and 0.5 mL of soapberry extract.

[0007] Preferably, the COSE@AgNPs microcapsules include the following raw materials: COSE@AgNPs, liquid paraffin, gelatin, gum arabic, an appropriate amount of 10% acetic acid solution, an appropriate amount of 20% sodium hydroxide solution, an appropriate amount of 37% formaldehyde solution, and distilled water, and the wall material wrapped around the outside of the raw materials is a mixture of gelatin and gum arabic.

[0008] Preferably, the preparation method of the COSE@AgNPs is as follows:

[0009] The dried cornus fruit core is roughly weighed and powdered, and ethanol is placed in an ultrasonically cleaned and dried conical flask to form a mixture, and the conical flask is placed in a refrigerator. The mixture is then extracted at an ultrasonic power of 360W and a temperature of 55°C, and filtered while hot, and evaporated using a rotary evaporator to obtain a concentrate, and the concentrate is centrifuged to obtain a supernatant, which is the cornus fruit core extract;

[0010] Mix 7.5mmol / L silver nitrate and 0.1g / mL cornus kernel extract, adjust the pH value to 8, and then perform ultrasound at a power of 320W and a temperature of 45°C for 30 minutes, followed by shaking on a shaker and additional ultraviolet irradiation.

[0011] A method for preparing a skin cleanser containing nano-silver microcapsules for sustained-release and antibacterial effects comprises the following steps:

[0012] Step 1: Add 50 mL of deionized water to a 250 mL beaker and heat the water bath to 70 °C.

[0013] Step 2: Add potassium cocamide glycinate and sodium lauroyl glutamate into the beaker, then place the beaker in a water bath and stir; after stirring thoroughly, add cocamidopropyl betaine and tea saponin, and stir with a glass rod until thoroughly mixed. Keep the temperature at 70°C during the stirring process;

[0014] Step 3: Add coconut oil fatty acid diethanolamide, fatty acid methyl ester ethoxylate, disodium EDTA, methyl paraben, and glycerol, and stir for 30 minutes until evenly mixed; after cooling to 50°C, add oat β-glucan, honeysuckle extract, and soapberry extract, and stir for 10 minutes until evenly mixed; adjust the pH value to 6.5 with citric acid, and then add COSE@AgNPs microcapsules.

[0015] Step 4: Add appropriate amount of sodium chloride to thicken, pour out, seal and package, and after standing, obtain the finished product of COSE@AgNPs microcapsule cleanser.

[0016] Microcapsule technology is a technology that uses materials such as polymers (capsule materials) to encapsulate solids, liquids or gases (core materials). The resulting particles are microcapsules. Microcapsule technology has many excellent functions, such as reducing the amount of antimicrobial agents used to reduce toxicity, reducing direct damage to the human body caused by antimicrobial agents, and achieving a slow release effect. Combining with antimicrobial nanosilver can prolong the antibacterial effect.

[0017] A skin cleanser containing nano silver microcapsules for sustained-release antibacterial effect is used for cleaning and antibacterial of human body surface, fatty acid methyl ester ethoxylate (FMEE) is used to have good hard water decontamination ability, the foam is relatively fine at the micron level, and the antibacterial effect of COSE@AgNPs microcapsules is long-lasting.

[0018] The nanosilver required by the present invention is prepared from an extract of cornus fruit kernels. The Chinese medicinal cornus is the dried mature pulp of cornus of the Cornaceae family. A large number of kernels produced during the ripening and harvesting of cornus fruit have not been effectively utilized, and the kernels are often discarded or even burned, which not only causes a huge waste of resources, but also damages the ecological environment. Therefore, the present invention uses cornus fruit kernel extract to prepare nanosilver particles (nanosilver particles are: COSE@AgNPs), which not only have broad-spectrum bactericidal properties, but also have good biocompatibility. The rational use of cornus fruit kernels is a systematic study and practice of resource value innovation, resource utilization and industrial development in the process of industrialization of traditional Chinese medicine resources, thereby reducing pollution to the environment and increasing the full utilization of resources.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The nanosilver prepared by COSE@AgNPs of the present invention from the extract of cornus fruit kernels not only has broad-spectrum bactericidal properties, but also has good biocompatibility. The rational use of cornus fruit kernels is a systematic study and practice of resource value innovation, resource utilization and industrial development in the process of industrialization of traditional Chinese medicine resources, thereby reducing pollution to the environment and increasing the full utilization of resources.

[0021] The COSE@AgNPs microcapsules of the present invention are applied to the preparation and application of a special cleansing lotion for coal mines. The microcapsules are made of gelatin-arabic gum as the capsule material, and COSE@AgNPs are used as the capsule core to make microcapsules and are innovatively applied to the cleansing lotion. When the cleansing lotion of the present invention is used for bathing, the nanosilver microcapsules are broken by slight touch or friction, and the nanosilver is slowly released, which not only prolongs the antibacterial effect of the nanosilver, but also avoids the irritation of the silver to the skin.

[0022] The present invention uses cornus officinalis kernel extract to prepare nanosilver (COSE@AgNPs) and uses microcapsule encapsulation technology to apply to skin cleansing lotion. The present invention finds that nanosilver microcapsules applied to skin cleansing lotion have obvious sustained-release and antibacterial effects, and fatty acid methyl ester ethoxylate (FMEE) has good hard water decontamination ability, and the foam is relatively fine at the micron level, which can keep the human skin surface clean for a long time. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 A is the dynamic light scattering technology image of COSE@AgNPs of the present invention, and B is the particle size distribution of COSE@AgNPs;

[0024] Figure 2 This is the electron microscope (TEM) image of COSE@AgNPs of the present invention;

[0025] Figure 3 The wavelength-absorbance curve of COSE@AgNPs of the present invention;

[0026] Figure 4 is the standard curve of COSE@AgNPs of the present invention;

[0027] Figure 5 A is the DPPH clearance rate of COSE@AgNPs of the present invention, and B is the ABST of COSE@AgNPs. + Clearance rate;

[0028] Figure 6 The wavelength-absorbance curve of the nanosilver, nanosilver microcapsules and hollow microcapsules of the present invention;

[0029] Figure 7 A is a bright field micrograph of the COSE@AgNPs microcapsules of the present invention, and B is a fluorescence micrograph of the COSE@AgNPs microcapsules;

[0030] Figure 8 A is the dynamic light scattering technology image of COSE@AgNPs microcapsules of the present invention, and B is the particle size distribution of COSE@AgNPs microcapsules;

[0031] Fig. 9The effects of different concentrations of the soapberry extract of the present invention on the cleansing lotion foam;

[0032] Fig.10 A is a micrograph of the foam of the cleansing lotion of the present invention, and B is a micrograph of the foam of a commercially available ordinary shower gel;

[0033] Fig.11 The effect of different concentrations of tea saponin on the foaming power of the cleansing lotion of the present invention;

[0034] Fig.12 is the ΔKP value of the surfactant of the present invention in hard water;

[0035] Fig.13 A is the antibacterial effect of the skin cleanser of the present invention on Staphylococcus aureus, and B is the antibacterial effect of the skin cleanser on Escherichia coli; wherein 1 is a skin cleanser, 2 is a nanosilver microcapsule skin cleanser, 3 is a nanosilver microcapsule + essential oil microcapsule skin cleanser, 4 is an essential oil microcapsule skin cleanser, and 5 is a commercially available shower gel; DETAILED DESCRIPTION

[0036] The following will disclose multiple embodiments of the present invention with diagrams. For the purpose of clear description, many physical details will be described together in the following description. However, it should be understood that these physical details should not be used to limit the present invention. In other words, in some embodiments of the present invention, these physical details are not necessary. In addition, for the purpose of simplifying the diagram, some conventional structures and components will be depicted in a simple schematic manner in the diagram.

[0037] In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the fact that ordinary technicians in the field can implement it. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0038] Example 1

[0039] The dried cornus kernels were crushed and sieved through 65 mesh. 20.0 g was accurately weighed and added into 200 mL of 50% ethanol by volume. The mixture was placed in an ultrasonically cleaned and dried conical flask, and soaked in a 4°C refrigerator for about 2 h. The mixture was extracted for 2 h at an ultrasonic power of 360 W and a temperature of 55°C. The mixture was filtered while hot and evaporated to 40 mL using a rotary evaporator. The resulting solution was further centrifuged at 5000 r / min for 10 min, and the supernatant was taken. The supernatant was the cornus kernel extract and stored in a 4°C refrigerator for later use.

[0040] Mix 7.5mmol / L silver nitrate and 0.1g / mL cornus officinalis kernel extract, adjust the pH to 8, and then use 320W power, 45℃ temperature, ultrasound for 30min, and then shake on a shaker and add UV irradiation for 5min. The solution changes from orange to brown, indicating that COSE@AgNPs are generated. The particle size of COSE@AgNPs is about 13.53-99.42nm, and the average particle size is 45.42nm. Figure 1 Scanning electron microscopy (TEM) images show that the generated COSE@AgNPs are highly dispersed, small-sized, nearly spherical particles, see Figure 2 .

[0041] Example 2

[0042] The preparation method of the COSE@AgNPs microcapsules of the present invention is as follows: liquid paraffin and nanosilver are fused, 6 mL of liquid paraffin and nanosilver mixed solution, 4 mL of distilled water, 2 g of gum arabic are prepared into colostrum according to the dry gel method, and then 0.5 g of gum arabic is added according to the prescription amount, and water is slowly added to 50 mL. Preparation of 5% gelatin solution: 2.5 g of gelatin + 50 mL of water is soaked and swollen (50°C water bath)

[0043] Transfer the solution into a beaker. Add 50 mL of 5% gelatin solution and place in a 50-55°C water bath, stir and mix. While stirring continuously, add 10% acetic acid solution to adjust the pH to 3.8. Add 200 mL of distilled water at 30°C, cool naturally until the temperature reaches about 30°C, place in an ice water bath to below 10°C, add 1.5 mL of 37% formaldehyde solution, stir for 15 minutes, and then adjust the pH to 8-9 with 20% sodium hydroxide, continue stirring for 20 minutes, let stand for a while, and filter to obtain solid COSE@AgNPs microcapsule powder.

[0044] Example 3

[0045] A skin cleanser containing COSE@AgNPs microcapsules for sustained-release and antibacterial effects is referred to as skin cleanser, which is composed of the following raw materials: potassium cocamide glycinate, sodium lauroyl glutamate, cocamidopropyl betaine, coconut oil fatty acid diethanolamide, fatty acid methyl ester ethoxylate, tea saponin, COSE@AgNPs microcapsules, glycerin, disodium EDTA, citric acid, deionized water, methyl paraben, oat β-glucan, honeysuckle extract, and soapberry extract. The COSE@AgNPs skin cleanser is prepared as follows:

[0046] Add 50 mL of deionized water to a 250 mL beaker and heat the water bath to 70 °C.

[0047] Add potassium cocamide glycinate and sodium lauroyl glutamate to the beaker. Place the beaker in a water bath and stir. After stirring thoroughly, add cocamidopropyl betaine and tea saponin, and stir with a glass rod until completely mixed. Keep the temperature at 70°C during the stirring process. Add coconut oil fatty acid diethanolamide, fatty acid methyl ester ethoxylate, disodium EDTA, glycerin, and methyl paraben and stir for 30 minutes until evenly mixed; after cooling to 50°C, add oat β-glucan, honeysuckle extract, soapberry extract, and appropriate amount of essence, and stir for 10 minutes until evenly mixed; adjust the pH value to 6.5 with citric acid, and add COSE@AgNPs microcapsule solid powder. Add appropriate amount of sodium chloride to thicken, pour out, seal and package, and get the finished cleanser after standing.

[0048] Example 4

[0049] Taking COSE@AgNPs as reference, the performance of COSE@AgNPs was characterized;

[0050] (1) Determination of characteristic peaks of COSE@AgNPs: Weigh a certain amount of COSE@AgNPs, dissolve and dilute with distilled water to prepare a COSE@AgNPs solution of a certain concentration, and use distilled water as a blank control. Perform full wavelength scanning in the range of 300-700 nm to determine the characteristic peaks of nanosilver, such as Figure 3 .

[0051] (2) Drawing of COSE@AgNPs standard curve: Weigh 0.03 g COSE@AgNPs into a 50 mL volumetric flask, dilute to volume with distilled water, shake well, and label the stock solution; accurately measure 20, 40, 60, 80, 100, and 120 μL of the stock solution into five 50 mL centrifuge tubes, label them 1, 2, 3, 4, 5, and 6 in sequence; shake well the five 50 mL centrifuge tubes, and use a pipette to draw 200 μL into a 96-well microplate. Measure the UV absorbance at the maximum absorption wavelength. Repeat three times and take the average value. Figure 4 .

[0052] (3) Free radical scavenging rate experiment of COSE@AgNPs

[0053] Determination of DPPH free radical scavenging rate: Weigh 0.005g of DPPH standard, dilute to 50.00mL with anhydrous ethanol, and obtain a mass concentration of 0.1mg / mL, which is recorded as solution A; take 24mL of A solution (0.1 mg / mL) and add 36mL of anhydrous ethanol to obtain 0.04mg / mL, which is recorded as solution B. Use a pipette to accurately measure 2mL of COSE@AgNPs solution (0.2, 0.4, 0.6, 0.8, 1.2, 1.4mg / mL) in a 10mL test tube, add the corresponding reagents in sequence, see Table 1, mix well, and stand in the dark for 30min. Take the supernatant and measure the absorbance of each at 517nm in a spectrophotometer, and use ascorbic acid solution (Vc) as a positive control.

[0054] Table 1 DPPH free radical scavenging rate determination sample addition table

[0055]

[0056] The DPPH free radical scavenging rate was calculated as follows:

[0057]

[0058] ABTS + Determination of free radical scavenging rate: Weigh 0.1918g ABTS and add distilled water to a 50mL volumetric flask. Take 0.0662g potassium persulfate and dilute to a 100mL volumetric flask with distilled water. Use a pipette to accurately measure the same volume of 7mmol / LABTS solution and 2.45mmol / L potassium persulfate solution, mix evenly, and react overnight in the dark for 12h to obtain ABTS storage solution, which is recorded as solution C. Use a pipette to accurately measure 2mL of COSE@AgNPs solution (0.02, 0.04, 0.06, 0.08, 0.12, 0.14mg / mL) in a 10mL test tube, add the corresponding reagents in sequence, see Table 2, mix evenly, let stand at room temperature for 10min, and measure the absorbance of each at 734nm in a spectrophotometer, with ascorbic acid solution (Vc) as a positive control.

[0059] Table 2 ABTS+ free radical scavenging rate determination sample addition table

[0060]

[0061] ABTS + The free radical scavenging rate is calculated as follows:

[0062]

[0063] The prepared COSE@AgNPs particles were coated with compounds (natural antioxidants) extracted from the kernels of Cornus officinalis, and they showed certain antioxidant properties. As the mass concentration of COSE@AgNPs increased from 0.2 mg / mL to 1.2 mg / mL, its ability to scavenge DPPH free radicals showed a significant enhancement trend, and its scavenging rate reached 78.42%. Figure 5 As shown in A. With the increase of concentration, the improvement of clearance rate is also continuously enhanced. There is a dose-effect relationship between the scavenging ability of COSE@AgNPs for DPPH free radicals and concentration. The IC50 of COSE@AgNPs for DPPH free radical scavenging rate is 62.84μg / mL. With the increase of COSE@AgNPs mass concentration, the scavenging rate of ABTS + The free radical scavenging capacity is on an upward trend. When the mass concentration of COSE@AgNPs is 120 μg / mL, its scavenging capacity for ABTS + The free radical scavenging ability is the strongest, reaching 65.86%. Figure 5 As shown in B, COSE@AgNPs reacts with ABTS + The IC50 of free radical scavenging rate is 87.31 μg / mL.

[0064] (3) Prepare microcapsules containing 9.6 mg / mL COSE@AgNPs and hollow microcapsules of the same concentration of COSE@AgNPs and compare them by measuring the absorbance using UV-visible light. Figure 6 , it was found that the characteristic peaks of COSE@AgNPs almost disappeared, proving that COSE@AgNPs had been encapsulated by microcapsules.

[0065] According to the method of Example 2, a hollow microcapsule and a COSE@AgNPs microcapsule were prepared. A nanosilver microcapsule was prepared, and COSE@AgNPs mass G1 was added thereto. Finally, the mass of the hollow microcapsule G2 and the mass of the COSE@AgNPs microcapsule G3 were weighed. The encapsulation efficiency of the COSE@Ag NPs microcapsule was calculated to be 82.03% by the following formula, indicating that the encapsulation effect was good and a large amount of COSE@AgNPs were encapsulated in the microcapsule.

[0066]

[0067] By using a fluorescence microscope and an inverted microscope to observe the COSE@AgNPs microcapsules, it was found that there was an obvious fluorescence reaction inside the microcapsules, indicating that the COSE@AgNPs were encapsulated in the microcapsules. Figure 7 .

[0068] The size of COSE@AgNPs microcapsules was observed by using a laser particle size analyzer. The particle size of the synthesized nanosilver microcapsules in the present invention is about 2 to 6 μm, with an average particle size of 4.078 μm. The particle size of most COSE@AgNPs microcapsules is distributed in the range of 3 to 5 μm, and the particle size distribution is relatively concentrated. Figure 8 .

[0069] The skin cleanser of the present invention uses the Chinese medicinal material soapberry extract, and the effect of the amount of the soapberry extract on the foam performance of the skin cleanser is explored through experiments.

[0070] The dosage of soapberry extract in this product experiment is 0%, 0.5%, 1%, 1.5%, 2%, 2.5%, and 3%. First, put 30mL of cleanser into a centrifuge tube, shake it for 5 minutes, and then calculate the volume of foam per minute. The experiment was used to test the effect of different contents of soapberry extract on the foaming volume of cleanser. Fig. 9 . According to the experimental results, when the concentration of soapberry extract is 1%, the foaming volume is the smallest, the foam is relatively fine and lasts the longest, and the stability is the best. Therefore, this experiment selects the soapberry extract with a concentration of 1% for the best effect. As the skin cleanser of the present invention, its foaming power is good, and the foam is at the micron level, reaching 53.04±4.59μm, which is significantly different from the control, as shown in Table 3 and Fig.10 .

[0071] Table 3 Foam particle size of cleansing lotion

[0072]

[0073] Note: ** indicates extremely significant (p value < 0.01).

[0074] The cleansing lotion of the present invention also uses a new herbal surfactant "tea saponin" to explore the effect of the amount of tea saponin on the performance of the cleansing lotion through experiments.

[0075] According to the experimental results, the dosage of tea saponin is proposed to be 1.0%, 2.0%, 4.0%, 6.0%, and 8.0%. First, 30mL of tea saponin of each concentration is placed in a centrifuge tube, shaken for 5 minutes, and then the volume of foam per minute is calculated. The experiment was used to test the effect of different contents of tea saponin on the foaming volume of the cleanser. Fig.11 The experimental results show that when the tea saponin concentration is 1.0%, the foaming volume is the largest, the duration is the longest, and the stability is the best. Therefore, in this experiment, the tea saponin concentration of 1.0% is the best.

[0076] Since the mineral ions in the water will have adverse effects on any kind of surfactant, it will seriously affect the performance of the cleanser. Since the calcium and magnesium ions in the water can combine with the hydrophilic group of the surfactant by van der Waals force, these calcium salts or magnesium salts will not only reduce the effective activity of the surfactant, but also increase the Krafft point KP of the surfactant. The more the Krafft point increases, the greater the decrease in the cleaning ability of the surfactant and the weaker the resistance to hard water. This experiment measured the hardness of the water used in the local coal mine in Pingdingshan City and selected three surfactants, fatty acid methyl ester ethoxylate (FMEE), fatty alcohol polyoxyethylene ether sodium sulfate (AES), and sodium dodecyl sulfate (K12), to compare their resistance to hard water. The results showed that the above surfactants were tested with water used on the coal mine site, and it was found that the Krafft value change (△KP) of fatty acid methyl ester ethoxylate was the smallest, indicating that FMEE has the strongest resistance to hard water, see Fig.12 .

[0077] The cleansing lotion can not only kill bacteria and inhibit bacteria, but also compare the various indicators of the cleansing lotion with other brands on the market. The results are shown in Table 4. It is found that the cleansing lotion has good detergency and foaming power, is mild and non-irritating, and is moisturizing.

[0078] Table 4 Comparison of physical and chemical properties of cleansing lotion

[0079]

[0080] The skin cleanser of the present invention was tested by selecting 50 volunteers to try the invention, and the various indicators of the skin cleanser were evaluated and the sensory index was evaluated by questionnaire scoring (scoring standard: unqualified: 2 points; qualified: 4 points; good: 6 points; good: 8 points; excellent: 10 points). Finally, the scoring results are summarized as shown in Table 5. Through the above data, it can be found that the overall sensory evaluation of the skin cleanser of this product is good.

[0081] Table 5 Sensory evaluation of cleansing lotion by volunteers

[0082]

[0083]

[0084] The present invention uses COSE@AgNPs microcapsules, also known as nanosilver microcapsules, and the nanosilver microcapsule cleansing lotion, nanosilver microcapsule + essential oil microcapsule cleansing lotion, cleansing lotion, essential oil microcapsule cleansing lotion and commercially available shower gel obtained according to the technical scheme to conduct antibacterial experiments on Staphylococcus aureus (S.aureus) and Escherichia coli (E.coli) using the "Oxford cup method". Fig.13 The antibacterial effects were compared by measuring the diameter of the inhibition zone.

[0085] It was found that the antibacterial effect of the present invention was good, especially the antibacterial effect of the nano-silver microcapsule + essential oil microcapsule cleanser was the best;

[0086] Take Staphylococcus aureus and Escherichia coli suspension 500 μ L respectively with pipette gun and add to sterilized culture dish, the agar medium after dissolving is cooled to about 50 ℃, pour into culture dish with aseptic operation, put horizontal position and rotate plate rapidly, after mixing, take Oxford cup and add nano silver microcapsule cleanser, nano silver microcapsule+essential oil microcapsule cleanser, cleanser, essential oil microcapsule cleanser, commercially available shower gel, each 50 μ L is placed on culture medium plate respectively, culture at 37 ℃, and measure the size of inhibition circle with vernier ruler. From table 6 data, it can be analyzed that the present invention has antibacterial effect for two kinds of bacteria. Compared with commercially available shower gel, the antibacterial effect of essential oil microcapsule cleanser, nano silver microcapsule cleanser, nano silver microcapsule+essential oil microcapsule cleanser reaches extremely significant difference, which proves that nano silver and essential oil are made into microcapsules and added to cleanser and there will be significant antibacterial effect.

[0087] Table 6 Evaluation table of the diameter of the antibacterial zone of different types of cleansing lotions

[0088]

[0089]

[0090] Note: ** indicates extremely significant (p value < 0.01).

[0091] The above description is only an embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A skin cleanser containing nano-silver microcapsules for sustained-release and antibacterial effects, characterized in that: The invention comprises the following raw materials: potassium cocamide glycinate, sodium lauroyl glutamate, cocamidopropyl betaine, coconut oil fatty acid diethanolamide, fatty acid methyl ester ethoxylate, tea saponin, COSE@AgNPs microcapsule, glycerin, disodium EDTA, citric acid, deionized water, methylparaben, oat β-glucan, honeysuckle extract and soapberry extract.

2. The cleansing lotion according to claim 1, characterized in that The proportions of each raw material component are as follows: potassium cocamide glycinate 4-8 mL, sodium lauroyl glutamate 2-4 mL, cocamidopropyl betaine 8-12 mL, coconut oil fatty acid diethanolamide 1-2 mL, fatty acid methyl ester ethoxylate 0.5-1 mL, tea saponin 0.8-1.2 g, COSE@AgNPs microcapsule 0.8-1.2 g, glycerol 4-6 g, disodium EDTA 0.08-0.12 g, citric acid 1-2 mL, deionized water 40-80 mL, methyl paraben 0.2 g, oat β-glucan 1.4-1.8 g, honeysuckle extract 0.3-0.7 g, and soapberry extract 0.3-0.7 mL.

3. The cleansing lotion according to claim 1, characterized in that The preferred proportions of each raw material component are as follows: 6 mL of potassium cocamide glycinate, 3 mL of sodium lauroyl glutamate, 10 mL of cocamidopropyl betaine, 1.5 mL of coconut oil fatty acid diethanolamide, 1 mL of fatty acid methyl ester ethoxylate, 1 g of tea saponin, 1 g of COSE@AgNPs microcapsules, 5 g of glycerol, 0.1 g of disodium EDTA, 1.5 mL of citric acid, 50 mL of deionized water, 0.2 g of methyl paraben, 1.6 g of oat β-glucan, 0.5 g of honeysuckle extract, and 0.5 mL of soapberry extract.

4. The cleansing lotion according to claim 1, characterized in that The COSE@AgNPs microcapsule comprises the following raw materials: COSE@AgNPs, liquid paraffin, gelatin, gum arabic, an appropriate amount of 10% acetic acid solution, an appropriate amount of 20% sodium hydroxide solution, 37% formaldehyde solution, and distilled water. The wall material wrapped outside the raw materials is a mixture of gelatin and gum arabic.

5. The skin cleanser according to claim 4, characterized in that: The preparation method of the COSE@AgNPs is as follows: The dried cornus fruit core is roughly weighed and powdered, and ethanol is placed in an ultrasonically cleaned and dried conical flask to form a mixture, and the conical flask is placed in a refrigerator. The mixture is then extracted at an ultrasonic power of 360W and a temperature of 55°C, and filtered while hot, and evaporated using a rotary evaporator to obtain a concentrate, and the concentrate is centrifuged to obtain a supernatant, which is the cornus fruit core extract; Mix 7.5mmol / L silver nitrate and 0.1g / mL cornus kernel extract, adjust the pH value to 8, and then perform ultrasound at a power of 320W and a temperature of 45°C for 30 minutes, followed by shaking on a shaker and additional ultraviolet irradiation.

6. A method for preparing a skin cleanser containing nano-silver microcapsules for sustained-release and antibacterial effects according to any one of claims 1 to 5, characterized in that: The steps include: Step 1: Add 50 mL of deionized water to a beaker and heat a water bath to 70°C. Step 2: Add potassium cocamide glycinate and sodium lauroyl glutamate into the beaker, then place the beaker in a water bath and stir; after stirring thoroughly, add cocamidopropyl betaine and tea saponin, and stir with a glass rod until thoroughly mixed. Keep the temperature at 70°C during the stirring process; Step 3: Add coconut oil fatty acid diethanolamide, fatty acid methyl ester ethoxylate, disodium EDTA, methyl paraben, and glycerol, and stir until uniformly mixed; after cooling to 50°C, add oat β-glucan, honeysuckle extract, and soapberry extract, and stir until uniformly mixed; adjust the pH value to 6.5 with citric acid, and then add COSE@AgNPs microcapsules; Step 4: Add appropriate amount of sodium chloride to thicken, pour out, seal and package, and after standing, obtain the finished product of COSE@AgNPs microcapsule cleanser.

7. Use of a skin cleanser containing nano-silver microcapsules for sustained-release antibacterial effect, characterized in that: The skin cleanser containing nano-silver microcapsules for sustained-release and antibacterial effects according to any one of claims 1 to 5 is used for cleaning and inhibiting bacteria on the surface of the human body.

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