Complete ammoxidation and anaerobic ammoxidation coupled denitrification process control device and method

Abstract

The invention discloses a complete ammoxidation and anaerobic ammoxidation coupled denitrification process control device and method, belongs to the technical field of control devices, and compared with a traditional denitrification technology, a COM-ANA synergetic denitrification technology has the advantages that the influence on the environment is small, the generated secondary pollution is less, the cost of an organic carbon source is saved, the denitrification efficiency is improved on the previous basis, and the environmental pollution is reduced. And moreover, the method has better adaptive capacity and can keep a stable treatment effect. According to the method, an experimental basis, a data basis, an experience basis and a theoretical basis are provided for a synergistic system of complete ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria, COM-ANA coupling enhanced nitrogen removal contribution, a nitrogen removal mechanism and a regulation and control principle are clarified, a new approach of microbial nitrogen removal is developed, and a new idea of a nitrogen removal bacteria synergistic nitrogen removal system is provided.

Description

Technical Field

[0001] The invention relates to a control device, in particular to a control device and method for a complete ammonia oxidation and anaerobic ammonia oxidation coupled denitrification process, belonging to the technical field of control devices. Background Art

[0002] Nitrogen is the main component of water eutrophication, and nitrogen pollution mainly comes from agricultural fertilization, industrial wastewater, domestic sewage and rainwater runoff.

[0003] Excess nitrogen can cause excessive growth of aquatic plants such as algae, consume oxygen in the water, lead to the death of fish and other aquatic life, and ultimately destroy the ecosystem.

[0004] Traditional chemical denitrification technology is widely used in wastewater treatment, but its shortcomings and limitations cannot be ignored.

[0005] For example, the denitrification process using chemical agents may release gases such as nitrogen oxides, produce toxic chemical by-products, and have high operating costs.

[0006] Microbial denitrification is a process that removes nitrogen pollutants from water bodies through microbial metabolic activities, which helps to restore damaged aquatic ecosystems, improve water quality and support the growth of aquatic organisms.

[0007] Microbial denitrification technology has been widely used in many fields. The relevant technologies and methods are relatively mature, with low operating costs, environmental protection and low carbon, and no secondary pollution, which is in line with my country's current concept of sustainable development.

[0008] With the deepening of research, new microbial denitrification technologies (such as anaerobic ammonia oxidation, complete ammonia oxidation, etc.) are constantly being developed, improving denitrification efficiency and treatment capacity.

[0009] COM-ANA technology is a new type of wastewater denitrification treatment technology that mainly uses the anaerobic ammonium oxidation process to efficiently remove nitrogen pollutants in wastewater. This technology combines the effects of anaerobic ammonium oxidizing bacteria and complete ammonia oxidizing bacteria, and can achieve efficient nitrogen removal at relatively low energy consumption.

[0010] The basic principle is that under anaerobic conditions, anaerobic ammonium oxidizing bacteria use ammonia nitrogen as an electron donor and nitrite nitrogen as an electron acceptor to carry out anaerobic ammonium oxidation reaction.

[0011] In the process of complete ammonia oxidizing bacteria consuming ammonia nitrogen to produce nitrate nitrogen, there will be a process of accumulation of nitrite nitrogen. Therefore, in the synergistic system with anaerobic ammonia oxidizing bacteria, it plays the role of providing nitrite for anaerobic ammonia oxidizing bacteria, and finally completely oxidizes ammonia nitrogen into nitrogen gas.

[0012] Compared with traditional nitrifying bacteria, complete ammonia oxidizing bacteria have a stronger affinity for ammonia nitrogen and are more active in anaerobic and oligotrophic states, so they are more compatible with anaerobic ammonium oxidizing bacteria. Compared with denitrifying bacteria, anaerobic ammonium oxidizing bacteria do not require additional organic carbon sources, which reduces costs, generates less residual sludge during the treatment process, reduces the burden of subsequent treatment, and avoids the production of intermediates.

[0013] Therefore, COM-ANA technology is an economical and environmentally friendly wastewater treatment technology that efficiently converts ammonia nitrogen and nitrite into nitrogen gas by utilizing anaerobic ammonium oxidation reaction. However, COM-ANA technology has high requirements on reaction conditions, such as temperature, pH value, ammonia nitrogen concentration, etc. Slight changes in these conditions may have a significant impact on the nitrogen removal effect, resulting in system instability. Therefore, a complete ammonia oxidation and anaerobic ammonia oxidation coupled denitrification process control device and method are designed to solve the above problems. Summary of the invention

[0014] The main purpose of the present invention is to provide a device and method for controlling a denitrification process of complete ammonia oxidation and anaerobic ammonia oxidation coupling.

[0015] The purpose of the present invention can be achieved by adopting the following technical solutions:

[0016] A denitrification process control device for coupling complete ammonia oxidation and anaerobic ammonia oxidation includes a magnetic stirrer, an outer shell is placed on the top of the magnetic stirrer, a rubber plug with holes is inserted into the top of the outer shell, and a detection group is connected to the reactor through a PLC monitor;

[0017] The outer shell is connected to the outlet peristaltic pump through the MBR membrane, and the outlet peristaltic pump is also connected to the outlet bucket;

[0018] The outer shell is connected to the water bath via a water bath peristaltic pump or directly connected to the water bath via a valve;

[0019] The reactor is connected to the water inlet barrel through a water inlet peristaltic pump.

[0020] Preferably, the detection group includes a pH meter, a temperature sensor, and a DO meter;

[0021] The PLC monitor is connected to the pH meter, the temperature sensor and the DO meter, and the other ends of the pH meter, the temperature sensor and the DO meter are inserted into the reactor through the rubber plug with holes.

[0022] Preferably, a biological filler is arranged inside the reactor, and a heat-insulating layer is arranged between the outer side of the reactor and the outer shell.

[0023] Preferably, a sampling tube is connected to the outer top of the reactor through a rubber plug with holes, and a rotor is arranged at the inner bottom of the reactor.

[0024] The control method of the denitrification process of complete ammonia oxidation and anaerobic ammonia oxidation coupling comprises the following steps:

[0025] Step 1: pH meter, temperature sensor, DO meter parameter test rod are inserted into the reactor through the rubber plug with holes, fixed by the iron frame, and the screen data is monitored by the PLC monitor. The MBR membrane is connected to the outlet pipe through the rubber plug with holes;

[0026] Step 2: Select activated sludge from the anaerobic ammonia oxidation activated sludge in the laboratory UASB process, with a VSS of 4-6 mg / L. Take out the granular activated sludge, take a certain amount of sludge sample, and then put the activated sludge and water into the reactor in equal proportion until the biological filler is immersed, and turn on the magnetic stirrer to speed up the stirring speed to turn the granular sludge into flocculent sludge, so that the sludge is inoculated on the filler as soon as possible, and then connect to new water for anaerobic culture in the dark;

[0027] The sludge samples collected during the period were subjected to total microbial DNA extraction using the FastDNA Spin Kit product, and specific nitrogen metabolism functional gene primers were used to perform polymerase chain reaction on Comammox, Anammox, AOB, AOA, NOB and Denitrifier, and then tested on a real-time quantitative PLC monitor;

[0028] Step 3: After the reactor is built, the temperature in the reactor is controlled by a water bath to be 31.0±1.6°C, the pH in the reactor is 7.3±1.6, no aeration treatment is performed during the process, the DO is 0.05±0.02, and the PLC monitor (13) monitors the screen data. The influent ammonia nitrogen concentration is adjusted to be stable at 60 mg / L, and the nitrite nitrogen concentration is gradually reduced to 0 mg / L after adjustment, thus promoting the formation of a synergistic system between complete ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria;

[0029] Step 4: Maintain the activity of complete ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria through continuous inlet and outlet water at the same time. According to the relationship between the inlet flow rate and the reaction volume, set an appropriate hydraulic retention time of 4h, and adjust the inlet ammonia nitrogen concentration and the inlet nitrite nitrogen concentration to ensure the synergistic system state of complete ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria. Use a UV-visible spectrophotometer to detect the inlet and outlet ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen concentrations to determine the reactor operation status and denitrification efficiency and make timely adjustments. Clean the MBR membrane regularly, observe the changes in the color and odor of the attachments, and calibrate the pH meter and DO meter regularly.

[0030] Beneficial technical effects of the present invention:

[0031] The complete ammonia oxidation and anaerobic ammonia oxidation coupled denitrification process control device and method provided by the present invention, compared with the traditional denitrification technology, the COM-ANA collaborative denitrification technology has less impact on the environment, produces less secondary pollution, saves the cost of organic carbon source, improves the denitrification efficiency on the basis of the previous one, and has good adaptability and can maintain a stable treatment effect. The method of the present invention provides an experimental basis, a data basis, an empirical basis and a theoretical basis for the complete ammonia oxidizing bacteria and the anaerobic ammonia oxidizing bacteria to achieve a synergistic system, clarifies the contribution of COM-ANA coupling to enhance denitrification, the denitrification mechanism and the regulation principle, opens up a new way for microbial denitrification, and provides a new idea for the denitrification system of denitrifying bacteria. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a structural schematic diagram of a preferred embodiment of a device and method for controlling a denitrification process coupled with complete ammonia oxidation and anaerobic ammonia oxidation according to the present invention.

[0033] In the figure: 1-reactor, 2-water inlet bucket, 3-water inlet peristaltic pump, 4-water outlet bucket, 5-water outlet peristaltic pump, 6-water bath, 7-water bath peristaltic pump, 8-magnetic stirrer, 9-pH meter, 10-temperature sensor, 11-DO meter, 12-MBR membrane, 13-PLC monitor, 14-biological filler, 15-sampling tube, 16-rubber plug with holes, 17-rotor, 18-insulation layer. DETAILED DESCRIPTION

[0034] In order to make the technical solution of the present invention more clear and specific to those skilled in the art, the present invention is further described in detail below in conjunction with embodiments and drawings, but the implementation manner of the present invention is not limited thereto.

[0035] Reactor 1, water inlet bucket 2, water inlet peristaltic pump 3, water outlet bucket 4, water outlet peristaltic pump 5, water bath 6, water bath peristaltic pump 7, magnetic stirrer 8, pH meter 9, DO meter 10, temperature sensor 11, PLC monitor 12, MBR membrane 13, biological filler 14.

[0036] The reactor 1 is a continuous flow MBBR reactor with a volume of 1.2L, wherein a stirring rotor and an external bottom magnetic stirrer 8 are provided at the bottom of the reactor, and a biological filler 14 is placed in the reactor 1, and the biological filler 14 used is a K5 carrier, which is filled to about 30% of the reaction volume, and the water inlet bucket 2 is connected to the water inlet peristaltic pump 3, and the water inlet peristaltic pump 3 is connected to the water inlet pipe and connected to the water inlet outside the reactor 1 wall, and the water bath 6 is connected to the water bath peristaltic pump 7 through a water pipe, and the water pipe is connected to insert into the outer annular insulation layer inside the reactor 1, and the insulation layer outlet is connected to the water pipe and inserted into the other end of the water bath 6 to form a heat preservation reflux. The parameter test rods of the pH meter 9, DO meter 10, and temperature sensor 11 extend into the reactor through a rubber plug with holes, are fixed by an iron frame, and the screen data is monitored by a plc monitor 12, and the MBR membrane 13 is connected to the outlet pipe through a rubber plug with holes.

[0037] 2. Sludge inoculation and microbial culture;

[0038] The activated sludge used was taken from the anaerobic ammonia oxidation activated sludge in the laboratory UASB process, with a VSS of 4-6 mg / L. The granular activated sludge was taken out, and a certain amount of sludge samples were taken. The activated sludge and the water were then put into the reactor in equal proportions until the filler was submerged, and the magnetic stirrer was turned on to speed up the stirring speed to make the granular sludge become flocculent sludge, so that the sludge was inoculated on the filler as soon as possible, and the new water was connected to the light-proof anaerobic culture. Because the sludge was well domesticated and the sludge activity was high, it was not screened or aerated before being put into the new reactor. The sludge samples taken during the period were used to extract the total microbial DNA using the FastDNA Spin Kit (Qbiogene, Carlsbad, CA) product, and the specific nitrogen metabolism functional gene primers were used to perform polymerase chain reaction (PCR) on Comammox, Anammox, AOB, AOA, NOB and Denitrifier, and then detected on a real-time quantitative PCR instrument (ABI, QuantStudio 5, US).

[0039] 3. The reaction system is running.

[0040] After the reactor was built, the water bath controlled the temperature inside the reactor to 31.0±1.6℃, the pH inside the reactor to 7.3±1.6, no aeration treatment was performed during the period, the DO was 0.05±0.02, and the PLC monitor monitored the screen data. The inlet ammonia nitrogen concentration was adjusted to stabilize at 60mg / L, and the nitrite nitrogen concentration was gradually reduced to 0mg / L after adjustment, thus promoting a synergistic system between complete ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria. At the same time, the activity of complete ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria is maintained by continuous inlet and outlet water. According to the relationship between the inlet flow rate and the reaction volume, an appropriate hydraulic retention time (HRT) of 4 hours is set, and the inlet ammonia nitrogen concentration and the inlet nitrite nitrogen concentration are adjusted to ensure the synergistic system state of complete ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria. The concentrations of inlet and effluent ammonia nitrogen, nitrite nitrogen and nitrate nitrogen are detected by ultraviolet-visible spectrophotometer to judge the operating status and denitrification efficiency of the reactor and make timely adjustments. The MBR membrane is cleaned regularly, the color and odor changes of the attachments are observed, and the dissolved oxygen meter and pH meter are calibrated regularly.

[0041] The above description is only a further embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solutions and concepts of the present invention within the scope disclosed by the present invention, which belong to the protection scope of the present invention.

Claims

1. A denitrification process control device for coupling complete ammonia oxidation and anaerobic ammonia oxidation, characterized by: It comprises a magnetic stirrer (8), an outer shell (19) is placed on the top of the magnetic stirrer (8), a rubber plug with holes (16) is inserted into the top of the outer shell (19), and a detection group is connected to the reactor (1) through a PLC monitor (13); The outer shell (19) is connected to the water outlet peristaltic pump (5) through the MBR membrane (12), and the water outlet peristaltic pump (5) is also connected to the water outlet bucket (4); The outer shell (19) is connected to the water bath (6) via a water bath peristaltic pump (7) or directly connected to the water bath (6) via a valve; The reactor (1) is connected to the water inlet barrel (2) via a water inlet peristaltic pump (3).

2. The device and method for controlling the denitrification process of total ammonia oxidation and anaerobic ammonia oxidation coupling according to claim 1, characterized in that: The detection group includes a pH meter (9), a temperature sensor (10), and a DO meter (11); The PLC monitor (13) is connected to the pH meter (9), the temperature sensor (10) and the DO meter (11), and the other ends of the pH meter (9), the temperature sensor (10) and the DO meter (11) are inserted into the reactor (1) through the perforated rubber plug (16).

3. The device for controlling the denitrification process of total ammonia oxidation and anaerobic ammonia oxidation coupling according to claim 2 is characterized in that: A biological filler (14) is arranged inside the reactor (1), and a heat-insulating layer (18) is arranged between the outer side of the reactor (1) and the outer shell (19).

4. The device for controlling the denitrification process of total ammonia oxidation and anaerobic ammonia oxidation coupling according to claim 3 is characterized in that: A sampling tube (15) is connected to the outer top of the reactor (1) through a rubber plug with holes (16), and a rotor (17) is arranged at the inner bottom of the reactor (1).

5. The method for controlling the denitrification process of total ammonia oxidation and anaerobic ammonia oxidation coupling according to claim 4, characterized in that: The steps include: Step 1: The parameter test rods of the pH meter (9), the temperature sensor (10), and the DO meter (11) are inserted into the reactor (1) through the rubber plug with holes (16), fixed by an iron frame, and the screen data is monitored by the PLC monitor (13). The MBR membrane (12) is connected to the water outlet pipe through the rubber plug with holes (16); Step 2: Select activated sludge from the anaerobic ammonium oxidation activated sludge in the laboratory UASB process, with a VSS of 4-6 mg / L. Take out the granular activated sludge, take a certain amount of sludge sample, and then put the activated sludge and the water into the reactor (1) in equal proportion until the biological filler (14) is immersed, and turn on the magnetic stirrer (8) to increase the stirring speed to make the granular sludge turn into flocculent sludge, so that the sludge is inoculated on the filler as soon as possible, and then connect to new water for anaerobic culture in the dark; The sludge samples collected during the period were used to extract total microbial DNA using the FastDNA Spin Kit product, and specific nitrogen metabolism functional gene primers were used for polymerase chain reaction for Comammox, Anammox, AOB, AOA, NOB and Denitrifier, and then detected on a real-time quantitative PLC monitor (13); Step 3: After the reactor (1) is built, the water bath (6) controls the temperature in the reactor (1) to be 31.0±1.6°C, the pH in the reactor (1) to be 7.3±1.6, no aeration treatment is performed during the process, the DO is 0.05±0.02, and the PLC monitor (13) monitors the screen data. The influent ammonia nitrogen concentration is adjusted to be stable at 60 mg / L, and the nitrite nitrogen concentration is adjusted to gradually decrease to 0 mg / L, thereby promoting the complete ammonia oxidizing bacteria and the anaerobic ammonia oxidizing bacteria to form a synergistic system; Step 4: Maintain the activity of complete ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria by continuous influent and effluent at the same time. According to the relationship between influent flow rate and reaction volume, set an appropriate hydraulic retention time of 4 hours, and adjust the influent ammonia nitrogen concentration and influent nitrite nitrogen concentration to ensure the coordinated system state of complete ammonia oxidizing bacteria and anaerobic ammonia oxidizing bacteria. Use UV-visible spectrophotometer to detect the influent and effluent ammonia nitrogen, nitrite nitrogen and nitrate nitrogen concentrations to judge the reactor operation state and denitrification efficiency and make timely adjustments. Clean the MBR membrane regularly (12), observe the changes in the color and odor of the attached matter, and calibrate the pH meter (9) and DO meter (11) regularly.

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