Winter Chinese cabbage bactericide and preparation method thereof

Abstract

The invention belongs to the field of botanical fungicide preparation, and particularly relates to a winter Chinese cabbage fungicide and a preparation method thereof. A potassium hydroxide solution is fully adsorbed by a prepared porous matrix through an esterification cross-linking reaction, an obtained efficient sterilization nanoparticle mixed viscous solution is extracted and concentrated to prepare a sterilization antiviral natural extract, and the prepared efficient sterilization suspension can effectively prevent and treat winter cabbage diseases; after polydimethylsiloxane and propylene glycol are mixed and emulsified, the disease-preventing and resistance-improving powder obtained through freeze drying is mixed in a formed breathable emulsion, and the prepared disease-preventing and resistance-improving composition can enable the Chinese cabbages in winter to maintain disease resistance for a long time; the mixture for promoting plant growth is uniformly mixed with the efficient sterilization component, and the prepared winter Chinese cabbage bactericide can efficiently prevent and treat diseases caused by pathogenic microorganisms, improves the disease resistance of plants, has biocompatibility and does not damage ecological balance.

Description

Technical Field

[0001] The invention belongs to the field of preparation of plant-derived fungicides, and specifically relates to a winter Chinese cabbage fungicide and a preparation method thereof. Background Art

[0002] Cabbage, as a vegetable with strong cold resistance, is an important edible crop in winter. It can grow normally at lower temperatures and can withstand temperatures as low as -3°C. The best time to plant winter cabbage is usually in autumn. Early-maturing varieties are sown in early to mid-August, medium- and late-maturing varieties are sown in late August to early September, and late-maturing varieties are sown in late August. Winter cabbage is rich in nutrients such as vitamin C, vitamin E, iron, potassium, vitamin A, etc. It is also rich in bioactive phytochemicals, such as glucosinolates, which have potential benefits such as antioxidant, antibacterial, and immune regulation.

[0003] However, the cold climate and humidity changes in winter also make cabbage susceptible to a variety of diseases. Downy mildew is one of the most common diseases of cabbage in winter, and its main manifestation is the appearance of a white mold layer on the back of the leaves. In severe cases, the leaves turn yellow and fall off. Soft rot mainly invades the cabbage through wounds, causing the tissue to soften and rot. Black spot disease mainly harms the leaves of cabbage, forming black spots and affecting photosynthesis. Viral disease is a major problem in cabbage production, which can cause symptoms such as mottled and wrinkled leaves. Cabbage dry heart disease begins to occur in the rosette stage, and dry edges appear on young leaves. The symptoms are more obvious in the heading stage, and the disease is mostly concentrated in the middle of the leaf head. The upper part of the leaf gradually becomes dry and yellow, the mesophyll becomes dry paper-like, and the veins are yellow-brown to dark black.

[0004] At present, the existing winter cabbage fungicide preparation technology has the following problems: first, the existing winter cabbage fungicide has no obvious control effect on diseases caused by bacteria, fungi and viruses, and the control effect on diseases is unstable. Most fungicides can only control diseases caused by a single microorganism; second, the existing winter cabbage fungicide is only effective for a certain period of time and cannot prevent and control cabbage diseases for a long time, and needs to be applied in large quantities for a long time; third, the existing winter cabbage fungicide falls into the soil, which will cause soil acidification, have an adverse effect on the beneficial microorganisms in the soil, and destroy the ecological balance. Summary of the invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a winter cabbage fungicide and a preparation method thereof. In order to solve the problems that the existing winter cabbage fungicide has no obvious control effect on diseases caused by pathogenic microorganisms, cannot control winter cabbage diseases for a long time, and destroys the ecological balance, the present invention uses a high-efficiency bactericidal suspension, a disease prevention and resistance enhancement composition, and a plant growth promoting mixture for mixing. The prepared winter cabbage fungicide can effectively control diseases caused by pathogenic microorganisms, maintain a bactericidal effect for a long time, has biocompatibility and does not destroy the ecological balance.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows: the present invention proposes a winter cabbage fungicide, and the raw materials for preparing the winter cabbage fungicide specifically include the following components in parts by weight: 70-90 parts of high-efficiency bactericidal suspension, 30-40 parts of disease prevention and resistance enhancement composition, 4-8 parts of methionine, 3-7 parts of methyl gallate, 6-9 parts of p-chlorophenoxyacetic acid, and 2-5 parts of allantoin.

[0007] Preferably, the raw materials for preparing the high-efficiency bactericidal suspension include the following components in parts by weight: 11-13 parts of Herba Euphorbiae Ichthyophthirius, 7-10 parts of Rhizoma Cyrthospermi, 8-12 parts of Viola yedoensis, 4-7 parts of Phyllanthus urinaria, 9-13 parts of potassium diformate, 26-33 parts of citrus fiber powder, 11-14 parts of citric acid, 1.8-2.6 parts of trisodium citrate, 8-12 parts of potassium hydroxide, and 12-16 parts of potassium alginate.

[0008] Preferably, the raw materials for preparing the disease prevention and resistance enhancement composition include the following components in parts by weight: 2-4 parts of salicylic acid, 3-5 parts of magnesium chloride, 3-6 parts of α-mannosidase, 17-24 parts of polydimethylsiloxane, 8-11 parts of propylene glycol, 25-31 parts of oxidized starch, and 13-16 parts of glutaraldehyde.

[0009] Preferably, the method for preparing the highly effective bactericidal suspension comprises the following steps: S1. Put Herba Dijincao, Cyperus rotundus, Viola yedoensis, and Herba Lycopodii into a pulverizer with a power of 1.5-1.8 kW, the pulverization temperature is 23-28°C, the pulverization time is 15-20 min, the pulverization speed is 3500-4500 r / min, and after pulverization, put it into an ultrasonic extractor with a power of 1.3-1.6 kW with a 50% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 40-45°C, the extraction time is 8-13 min, and after extraction, put it into a herbal concentrator with a power of 2.5-3.5 kW, the concentration temperature is 40-60°C, the concentration time is 35-45 min, and the concentration speed is 10-20 r / min to obtain a bactericidal and antiviral natural extract; S2, put potassium diformate and ultrapure water into a stirring tank with a power of 1.5-1.8 kW, the stirring temperature is 25-30°C, the stirring time is 8-12 min, the stirring speed is 150-180 r / min, and after complete dissolution, a potassium diformate solution is obtained; S3, placing the citrus fiber powder, the potassium diformate solution prepared in S2, citric acid, trisodium citrate, and N-methylpyrrolidone into a reactor with a power of 2.2-2.5 kW, the reaction temperature is 90-100°C, the reaction time is 1.3-1.6 h, the reaction speed is 200-300 r / min, and an esterification cross-linking reaction is performed to obtain a porous matrix; S4, put potassium hydroxide and ultrapure water into a magnetic stirrer with a power of 1.6-1.8 kW, the stirring temperature is 28-33°C, the stirring time is 10-15 min, the stirring speed is 320-350 r / min, after dissolution, add the porous matrix prepared by S3, the stirring temperature is 25-30°C, the stirring time is 20-25 min, the stirring speed is 400-600 r / min, stir for sufficient adsorption, put into a vacuum freeze nano spray dryer with a power of 2.3-2.8 kW, the vacuum degree is -0.05 MPa, the freeze nano spray drying temperature is -40°C, the freeze nano spray drying particle size is 100 nm, the freeze nano spray drying time is 2.5-3h, freeze spray drying is performed to obtain highly efficient bactericidal nanoparticles; S5. Put potassium alginate and ultrapure water into a stirring tank with a power of 1.5-1.8 kW, stir at a temperature of 60-80°C, stir for 10-15 min, and stir at a speed of 200-240 r / min to obtain a viscous solution; S6. Put the bactericidal and antiviral natural extract prepared by S1 and the high-efficiency bactericidal nanoparticles prepared by S4 into the viscous solution prepared by S5, stir at a temperature of 30-36°C, for 13-18 min, at a speed of 260-280 r / min, and stir to mix to obtain a high-efficiency bactericidal suspension.

[0010] Furthermore, in S1, the solid-liquid ratio of the Herba Euphorbiae Ichthyophthirius and the 50% ethanol solution is 1.5:1 g / mL, and in S2, the mass fraction of the potassium diformate in ultrapure water is 35-39%.

[0011] Furthermore, in S3, the solid-liquid ratio of the citrus fiber powder to N-methylpyrrolidone is 0.2:1 g / mL, in S4, the mass fraction of the potassium hydroxide in ultrapure water is 36-42%, and in S5, the mass fraction of the potassium alginate in ultrapure water is 2-4%.

[0012] Preferably, the method for preparing the disease prevention and resistance enhancement composition specifically comprises the following steps: L1. Put salicylic acid, magnesium chloride, α-mannosidase and 50% ethanol solution into a stirrer with a power of 1.2-1.5 kW, stir at a temperature of 23-28°C, stir for 10-15 min, stir at a speed of 180-220 r / min, mix and dissolve to obtain a disease resistance enhancing preparation; L2, putting polydimethylsiloxane and propylene glycol into a high-speed stirrer with a power of 2.5 kW, the high-speed stirring temperature is 25-30°C, the high-speed stirring speed is 6000-8000 r / min, the high-speed stirring time is 20-30 min, and high-speed stirring is emulsified to obtain a breathable emulsion; L3, putting oxidized starch, glutaraldehyde and ultrapure water into a reactor with a power of 2.7-2.9 kW, reacting at a temperature of 50°C, a reaction speed of 200 r / min, and a reaction time of 2.1-2.3 h to obtain a microporous foaming material; L4, putting the microporous foam material prepared by L3 into the disease resistance enhancement preparation prepared by L1, stirring at a temperature of 23-28°C, stirring for 14-18 min, stirring at a speed of 230-250 r / min, mixing, putting into a freeze dryer with a power of 1.6-2.2 kW, freeze drying pressure of 300 Pa, reduced pressure drying temperature of -45°C, freeze drying for 12 h, heating to -25°C and freeze drying for 8 h, freeze drying at 15°C for 5 h, to obtain a disease prevention and resistance enhancement powder; L5. Put the disease-preventing and resistance-enhancing powder prepared by L4 into the breathable emulsion prepared by L2, and put it into an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min, and perform ultrasonic dispersion to obtain a disease-preventing and resistance-enhancing composition.

[0013] Furthermore, in L1, the solid-liquid ratio of salicylic acid to 50% ethanol solution is 0.15:1 g / mL.

[0014] Furthermore, in L3, the mass fraction of glutaraldehyde in ultrapure water is 20-28%.

[0015] The present invention also provides a method for preparing a winter cabbage fungicide, which specifically comprises the following steps: Step 1, putting methionine, methyl gallate, p-chlorophenoxyacetic acid, allantoin and 70% ethanol solution into a stirring tank with a power of 1.5-1.8 kW, stirring at a temperature of 70-80° C., stirring for 14-19 min, and stirring at a speed of 200-240 r / min to mix to obtain a mixture for promoting plant growth; Step 2: Put the high-efficiency bactericidal suspension and the disease prevention and resistance enhancement composition into a stirrer with a power of 1.8-2.1 kW, stir at a temperature of 30-38°C, stir for 20-30 min, and stir at a speed of 300-400 r / min to mix and obtain a high-efficiency bactericidal component; Step 3: Add the plant growth promoting mixture prepared in step 1 to the high-efficiency bactericidal component prepared in step 2, stir at a temperature of 25-35°C, stir for 18-26 min, stir at a speed of 300-400 r / min, mix evenly, and obtain a winter cabbage bactericide.

[0016] Preferably, in step 1, the solid-liquid ratio of methionine to 70% ethanol solution is 0.13:1 g / mL.

[0017] The beneficial effects achieved by the present invention are as follows: The invention adopts Herba Euphorbiae Herba, Rhizoma Cyrthospermi, Viola yedoensis and Phyllanthus urinariae, and grinds them for extraction and concentration. The obtained bactericidal and antiviral natural extract contains isoquercetin, euphorbin, brucine, strychnine, disulfide, esculentol, ferulic acid, phyllanthus urinariae and other compounds with broad-spectrum bactericidal, antiviral and biocompatible properties. Potassium diformate solution and citrus fiber powder are mixed with citric acid as a cross-linking agent. Under the catalytic action of trisodium citrate, carboxyl groups on citric acid and potassium diformate react with hydroxyl groups in molecules of citrus fiber powder to form ester bonds, thereby forming a porous matrix with bactericidal, antiviral and adsorption properties. Potassium hydroxide cooperates with potassium diformate to destroy and penetrate the cell walls of bacteria and fungi through hydrolysis, change the permeability of bacterial and fungal cell membranes, and denature the proteins on the surface of viruses. After potassium alginate is dissolved in ultrapure water, the viscous solution prepared can adhere to the peptidoglycan of bacterial cell walls, the chitin of fungal cell walls, and the glycoprotein on the surface of viruses. The highly efficient bactericidal nanoparticles that fully absorb the potassium hydroxide solution are mixed with the viscous solution and the bactericidal and antiviral natural extracts. The prepared highly efficient bactericidal suspension can effectively kill parasitic downy mildew, carrot soft rot pectin bacteria, pineapple pantoea, and butyric acid bacteria that cause winter cabbage diseases. Pseudomonas aeruginosa, tobacco mosaic virus and other pathogenic microorganisms, and does not harm the cabbage itself; salicylic acid, magnesium chloride and α-mannosidase are mixed to obtain a disease resistance-enhancing preparation that can regulate the activity of enzymes contained in winter cabbage, induce the production of reactive oxygen and activate plant immune response, thereby enhancing the disease resistance of winter cabbage itself; after polydimethylsiloxane and propylene glycol are mixed and emulsified, the formed breathable emulsion is air-dried into a film, which has air permeability and can prevent plants from suffering from frost damage; the hydroxyl groups in the oxidized starch molecules and the aldehyde groups in the glutaraldehyde molecules undergo a condensation reaction, and the obtained microporous foaming material absorbs and enhances the disease resistance preparation The prepared disease prevention and resistance enhancement powder is ultrasonically dispersed in a breathable emulsion, and the prepared disease prevention and resistance enhancement composition can enable winter cabbage to maintain disease resistance for a long time; after methionine, methyl gallate, p-chlorophenoxyacetic acid, and allantoin are mixed, the prepared plant growth promotion mixture can promote plant rooting, enhance plant nutrient absorption, increase plant growth rate and dry matter accumulation, and is mixed with a high-efficiency bactericidal component. The prepared winter cabbage bactericide can effectively prevent and control diseases caused by pathogenic microorganisms, does not harm plant leaves, maintains a long-term bactericidal effect, enhances the disease resistance of the plant itself, has biocompatibility, and does not destroy the ecological balance. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the present invention or the technical solutions in the prior art, the following will be described in a clear and understandable manner in conjunction with the accompanying drawings. Obviously, the drawings described below are only for the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 This is a scanning electron microscope image of the internal structure of the highly effective bactericidal nanoparticles described in Experimental Example 1 of the present invention; Figure 2 This is a graph showing the effect of controlling downy mildew on Chinese cabbage described in Experimental Example 1 of the present invention; Figure 3 This is a graph showing the effect of preventing and controlling the soft rot of Chinese cabbage described in Experimental Example 1 of the present invention; Figure 4 This is a result diagram of the control effect of Chinese cabbage mosaic disease described in Experimental Example 1 of the present invention; Figure 5 This is a graph showing the soil pH value measurement results described in Experimental Example 2 of the present invention; Figure 6 This is a graph showing the results of determining the bacterial colony count in the soil described in Experimental Example 2 of the present invention. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments; based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0021] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0022] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials and test strains used in the following examples are purchased from commercial channels unless otherwise specified.

[0023] Example 1: This example provides a winter cabbage fungicide and a preparation method thereof. The winter cabbage fungicide comprises the following components in parts by weight: 70 parts of high-efficiency bactericidal suspension, 30 parts of disease prevention and resistance enhancement composition, 4 parts of methionine, 3 parts of methyl gallate, 6 parts of p-chlorophenoxyacetic acid, and 2 parts of allantoin.

[0024] The raw materials for preparing the high-efficiency bactericidal suspension include the following components in parts by weight: 11 parts of Herba Euphorbiae Ichthyophthirius, 7 parts of Rhizoma Cyrthospermi, 8 parts of Viola yedoensis, 4 parts of Phyllanthus urinaria, 9 parts of potassium diformate, 26 parts of citrus fiber powder, 11 parts of citric acid, 1.8 parts of trisodium citrate, 8 parts of potassium hydroxide, and 12 parts of potassium alginate.

[0025] The raw materials for preparing the disease prevention and resistance enhancement composition include the following components in parts by weight: 2 parts of salicylic acid, 3 parts of magnesium chloride, 3 parts of α-mannosidase, 17 parts of polydimethylsiloxane, 8 parts of propylene glycol, 25 parts of oxidized starch, and 13 parts of glutaraldehyde.

[0026] The preparation method of the highly effective bactericidal suspension specifically comprises the following steps: S1. Put Herba Dijincao, Cyperus rotundus, Viola yedoensis, and Herba Lycopodii into a 1.5 kW grinder, the grinding temperature is 23°C, the grinding time is 15 min, the grinding speed is 3500 r / min, and after grinding, put them into an ultrasonic extractor with a power of 1.3 kW and a 50% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 40°C, the extraction time is 8 min, and after extraction, put them into a herbal concentrator with a power of 2.5 kW, the concentration temperature is 40°C, the concentration time is 35 min, and the concentration speed is 10 r / min to obtain a bactericidal and antiviral natural extract; S2, put potassium diformate and ultrapure water into a stirring tank with a power of 1.5 kW, the stirring temperature is 25°C, the stirring time is 8 min, the stirring speed is 150 r / min, and after complete dissolution, a potassium diformate solution is obtained; S3, putting the citrus fiber powder, the potassium diformate solution prepared in S2, citric acid, trisodium citrate, and N-methylpyrrolidone into a reactor with a power of 2.2 kW, the reaction temperature is 90°C, the reaction time is 1.3 h, the reaction speed is 200 r / min, and an esterification cross-linking reaction is carried out to obtain a porous matrix; S4, put potassium hydroxide and ultrapure water into a magnetic stirrer with a power of 1.6 kW, the stirring temperature is 28°C, the stirring time is 10 min, the stirring speed is 320 r / min, after dissolution, add the porous matrix prepared by S3, the stirring temperature is 25°C, the stirring time is 20 min, the stirring speed is 400 r / min, stir for sufficient adsorption, put into a vacuum freeze nano spray dryer with a power of 2.3 kW, the vacuum degree is -0.05 MPa, the freeze nano spray drying temperature is -40°C, the freeze nano spray drying particle size is 100 nm, the freeze nano spray drying time is 2.5 h, freeze spray drying is performed to obtain highly efficient bactericidal nanoparticles; S5. Put potassium alginate and ultrapure water into a stirring tank with a power of 1.5 kW, stir at a temperature of 60°C, for 10 min, at a stirring speed of 200 r / min, and stir evenly to obtain a viscous solution; S6. Put the bactericidal and antiviral natural extract prepared by S1 and the high-efficiency bactericidal nanoparticles prepared by S4 into the viscous solution prepared by S5, stir at a temperature of 30°C, for 13 min, at a speed of 260 r / min, and stir to mix to obtain a high-efficiency bactericidal suspension.

[0027] In S1, the solid-liquid ratio of the Herba Euphorbiae Ichthyophthirius and the 50% ethanol solution is 1.5:1 g / mL, and in S2, the mass fraction of the potassium diformate in ultrapure water is 35%.

[0028] In S3, the solid-liquid ratio of the citrus fiber powder to N-methylpyrrolidone is 0.2:1 g / mL, in S4, the mass fraction of the potassium hydroxide in ultrapure water is 36%, and in S5, the mass fraction of the potassium alginate in ultrapure water is 2%.

[0029] The method for preparing the disease prevention and resistance enhancement composition specifically comprises the following steps: L1. Put salicylic acid, magnesium chloride, α-mannosidase and 50% ethanol solution into a 1.2 kW stirrer, stir at 23°C, stir for 10 min, stir at 180 r / min, mix and dissolve to obtain a disease resistance enhancement preparation; L2, put polydimethylsiloxane and propylene glycol into a high-speed stirrer with a power of 2.5 kW, the high-speed stirring temperature is 25°C, the high-speed stirring speed is 6000 r / min, the high-speed stirring time is 20 min, and high-speed stirring emulsifies to obtain a breathable emulsion; L3, putting oxidized starch, glutaraldehyde and ultrapure water into a reactor with a power of 2.7 kW, reacting at a temperature of 50°C, a reaction speed of 200 r / min, and a reaction time of 2.1 h to obtain a microporous foaming material; L4, put the microporous foam material prepared by L3 into the disease resistance enhancement preparation prepared by L1, stir at 23°C for 14 min, stir at 230 r / min, mix, put into a freeze dryer with a power of 1.6 kW, freeze dry at a pressure of 300 Pa, and a reduced pressure drying temperature of -45°C, freeze dry for 12 h, heat to -25°C and freeze dry for 8 h, and freeze dry at 15°C for 5 h to obtain a disease prevention and resistance enhancement powder; L5. Put the disease-preventing and resistance-enhancing powder prepared by L4 into the breathable emulsion prepared by L2, and put it into an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min, and perform ultrasonic dispersion to obtain a disease-preventing and resistance-enhancing composition.

[0030] In L1, the solid-liquid ratio of salicylic acid to 50% ethanol solution is 0.15:1 g / mL.

[0031] In L3, the mass fraction of glutaraldehyde in ultrapure water is 20%.

[0032] This embodiment also provides a method for preparing a winter cabbage fungicide, which specifically comprises the following steps: Step 1: put methionine, methyl gallate, p-chlorophenoxyacetic acid, allantoin and 70% ethanol solution into a stirring tank with a power of 1.5 kW, stir at a temperature of 70° C., stir for 14 min, and stir at a speed of 200 r / min to obtain a mixture for promoting plant growth; Step 2: Put the high-efficiency bactericidal suspension and the disease prevention and resistance enhancement composition into a stirrer with a power of 1.8 kW, stir at a temperature of 30° C., stir for 20 min, and stir at a speed of 300 r / min to mix and obtain a high-efficiency bactericidal component; Step 3: Add the plant growth promoting mixture prepared in step 1 to the high-efficiency bactericidal component prepared in step 2, stir at a temperature of 25°C, stir for 18 min, stir at a speed of 300 r / min, mix evenly, and obtain a winter cabbage bactericide.

[0033] In step 1, the solid-liquid ratio of methionine to 70% ethanol solution is 0.13:1 g / mL.

[0034] Example 2: This example provides a winter cabbage fungicide and a preparation method thereof. The winter cabbage fungicide comprises the following components in parts by weight: 80 parts of high-efficiency bactericidal suspension, 35 parts of disease prevention and resistance enhancement composition, 6 parts of methionine, 5 parts of methyl gallate, 8 parts of p-chlorophenoxyacetic acid, and 4 parts of allantoin.

[0035] The raw materials for preparing the high-efficiency bactericidal suspension include the following components in parts by weight: 12 parts of Herba Euphorbiae Ichthyophthirius, 8 parts of Rhizoma Cyrthospermi, 10 parts of Viola yedoensis, 6 parts of Phyllanthus urinaria, 11 parts of potassium diformate, 30 parts of citrus fiber powder, 12 parts of citric acid, 2.2 parts of trisodium citrate, 10 parts of potassium hydroxide, and 14 parts of potassium alginate.

[0036] The raw materials for preparing the disease prevention and resistance enhancement composition include the following components in parts by weight: 3 parts of salicylic acid, 4 parts of magnesium chloride, 5 parts of α-mannosidase, 21 parts of polydimethylsiloxane, 10 parts of propylene glycol, 28 parts of oxidized starch, and 15 parts of glutaraldehyde.

[0037] The preparation method of the highly effective bactericidal suspension specifically comprises the following steps: S1. Put Herba Dijincao, Rhizoma Cynomorii, Viola yedoensis, and Herba Lycopodii into a 1.6 kW grinder, the grinding temperature is 26°C, the grinding time is 18 min, the grinding speed is 4000 r / min, and after grinding, put them into an ultrasonic extractor with a power of 1.4 kW and a 50% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 43°C, the extraction time is 11 min, and after extraction, put them into a herbal concentrator with a power of 2.8 kW, the concentration temperature is 50°C, the concentration time is 40 min, and the concentration speed is 15 r / min to obtain a bactericidal and antiviral natural extract; S2, put potassium diformate and ultrapure water into a stirring tank with a power of 1.6 kW, the stirring temperature is 28°C, the stirring time is 10 min, the stirring speed is 160 r / min, and after complete dissolution, a potassium diformate solution is obtained; S3, putting the citrus fiber powder, the potassium diformate solution prepared in S2, citric acid, trisodium citrate, and N-methylpyrrolidone into a reactor with a power of 2.3 kW, the reaction temperature is 95°C, the reaction time is 1.4 h, the reaction speed is 200 r / min, and an esterification cross-linking reaction is carried out to obtain a porous matrix; S4, put potassium hydroxide and ultrapure water into a magnetic stirrer with a power of 1.7 kW, the stirring temperature is 30°C, the stirring time is 13 min, the stirring speed is 330 r / min, after dissolving, add the porous matrix prepared by S3, the stirring temperature is 28°C, the stirring time is 23 min, the stirring speed is 500 r / min, stir for sufficient adsorption, put into a vacuum freeze nano spray dryer with a power of 2.6 kW, the vacuum degree is -0.05 MPa, the freeze nano spray drying temperature is -40°C, the freeze nano spray drying particle size is 100 nm, the freeze nano spray drying time is 2.8 h, freeze spray drying is performed to obtain highly efficient bactericidal nanoparticles; S5. Put potassium alginate and ultrapure water into a stirring tank with a power of 1.6 kW, stir at a temperature of 70°C, for 13 min, at a stirring speed of 220 r / min, and stir evenly to obtain a viscous solution; S6. Put the bactericidal and antiviral natural extract prepared by S1 and the high-efficiency bactericidal nanoparticles prepared by S4 into the viscous solution prepared by S5, stir at a temperature of 33°C, for 15 min, at a speed of 270 r / min, and stir to mix to obtain a high-efficiency bactericidal suspension.

[0038] In S1, the solid-liquid ratio of the Herba Euphorbiae Ichthyophthirius and the 50% ethanol solution is 1.5:1 g / mL, and in S2, the mass fraction of the potassium diformate in ultrapure water is 36%.

[0039] In S3, the solid-liquid ratio of the citrus fiber powder to N-methylpyrrolidone is 0.2:1 g / mL, in S4, the mass fraction of the potassium hydroxide in ultrapure water is 39%, and in S5, the mass fraction of the potassium alginate in ultrapure water is 3%.

[0040] The method for preparing the disease prevention and resistance enhancement composition specifically comprises the following steps: L1. Put salicylic acid, magnesium chloride, α-mannosidase and 50% ethanol solution into a 1.3 kW stirrer, stir at 26°C, stir for 13 min, stir at 200 r / min, mix and dissolve to obtain a disease resistance enhancement preparation; L2, put polydimethylsiloxane and propylene glycol into a high-speed stirrer with a power of 2.5 kW, the high-speed stirring temperature is 28°C, the high-speed stirring speed is 7000 r / min, the high-speed stirring time is 25 min, and high-speed stirring emulsifies to obtain a breathable emulsion; L3, putting oxidized starch, glutaraldehyde and ultrapure water into a reactor with a power of 2.8 kW, reacting at a temperature of 50°C, a reaction speed of 200 r / min, and a reaction time of 2.2 h to obtain a microporous foaming material; L4, put the microporous foam material prepared by L3 into the disease resistance enhancement preparation prepared by L1, stir at 26°C, stir for 16 min, stir at 240 r / min, mix, put into a freeze dryer with a power of 1.9 kW, freeze dry at a pressure of 300 Pa, and a reduced pressure drying temperature of -45°C, freeze dry for 12 h, heat to -25°C and freeze dry for 8 h, and freeze dry at 15°C for 5 h to obtain a disease prevention and resistance enhancement powder; L5. Put the disease-preventing and resistance-enhancing powder prepared by L4 into the breathable emulsion prepared by L2, and put it into an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min, and perform ultrasonic dispersion to obtain a disease-preventing and resistance-enhancing composition.

[0041] In L1, the solid-liquid ratio of salicylic acid to 50% ethanol solution is 0.15:1 g / mL.

[0042] In L3, the mass fraction of glutaraldehyde in ultrapure water is 25%.

[0043] This embodiment also provides a method for preparing a winter cabbage fungicide, which specifically comprises the following steps: Step 1: put methionine, methyl gallate, p-chlorophenoxyacetic acid, allantoin and 70% ethanol solution into a stirring tank with a power of 1.6 kW, stirring at a temperature of 75° C., stirring for 18 min, and stirring at a speed of 230 r / min to obtain a mixture that promotes plant growth; Step 2: Put the high-efficiency bactericidal suspension and the disease prevention and resistance enhancement composition into a stirrer with a power of 1.9 kW, stir at a temperature of 34° C., stir for 25 min, and stir at a speed of 300 r / min to mix and obtain a high-efficiency bactericidal component; Step 3: Add the plant growth promoting mixture prepared in step 1 to the high-efficiency bactericidal component prepared in step 2, stir at a temperature of 30°C, stir for 22 min, stir at a speed of 300 r / min, mix evenly, and obtain a winter cabbage bactericide.

[0044] In step 1, the solid-liquid ratio of methionine to 70% ethanol solution is 0.13:1 g / mL.

[0045] Example 3: This example provides a winter cabbage fungicide and a preparation method thereof. The winter cabbage fungicide comprises the following components in parts by weight: 90 parts of high-efficiency bactericidal suspension, 40 parts of disease prevention and resistance enhancement composition, 8 parts of methionine, 7 parts of methyl gallate, 9 parts of p-chlorophenoxyacetic acid, and 5 parts of allantoin.

[0046] The raw materials for preparing the high-efficiency bactericidal suspension include the following components in parts by weight: 13 parts of Herba Euphorbiae Ichthyophthirius, 10 parts of Rhizoma Cyrthospermi, 12 parts of Viola yedoensis, 7 parts of Phyllanthus urinaria, 13 parts of potassium diformate, 33 parts of citrus fiber powder, 14 parts of citric acid, 2.6 parts of trisodium citrate, 12 parts of potassium hydroxide, and 16 parts of potassium alginate.

[0047] The raw materials for preparing the disease prevention and resistance enhancement composition include the following components in parts by weight: 4 parts of salicylic acid, 5 parts of magnesium chloride, 6 parts of α-mannosidase, 24 parts of polydimethylsiloxane, 11 parts of propylene glycol, 31 parts of oxidized starch, and 16 parts of glutaraldehyde.

[0048] The preparation method of the highly effective bactericidal suspension specifically comprises the following steps: S1. Put Herba Dijincao, Rhizoma Cynomorii, Viola yedoensis, and Herba Lycopodii into a 1.8 kW grinder, the grinding temperature is 28°C, the grinding time is 20 min, the grinding speed is 4500 r / min, and after grinding, put them into an ultrasonic extractor with a power of 1.6 kW and a 50% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 45°C, the extraction time is 13 min, and after extraction, put them into a herbal concentrator with a power of 3.5 kW, the concentration temperature is 60°C, the concentration time is 45 min, and the concentration speed is 20 r / min to obtain a bactericidal and antiviral natural extract; S2, put potassium diformate and ultrapure water into a stirring tank with a power of 1.8 kW, the stirring temperature is 30°C, the stirring time is 12 min, the stirring speed is 180 r / min, and after complete dissolution, a potassium diformate solution is obtained; S3, putting the citrus fiber powder, the potassium diformate solution prepared in S2, citric acid, trisodium citrate, and N-methylpyrrolidone into a reactor with a power of 2.5 kW, the reaction temperature is 100°C, the reaction time is 1.6 h, the reaction speed is 300 r / min, and an esterification cross-linking reaction is carried out to obtain a porous matrix; S4, put potassium hydroxide and ultrapure water into a magnetic stirrer with a power of 1.8 kW, the stirring temperature is 33°C, the stirring time is 15 min, the stirring speed is 350 r / min, after dissolution, add the porous matrix prepared by S3, the stirring temperature is 30°C, the stirring time is 25 min, the stirring speed is 600 r / min, stir for sufficient adsorption, put into a vacuum freeze nano spray dryer with a power of 2.8 kW, the vacuum degree is -0.05 MPa, the freeze nano spray drying temperature is -40°C, the freeze nano spray drying particle size is 100 nm, the freeze nano spray drying time is 3 h, freeze spray drying is performed to obtain highly efficient bactericidal nanoparticles; S5. Put potassium alginate and ultrapure water into a stirring tank with a power of 1.8 kW, stir at a temperature of 80°C, for 15 min, at a stirring speed of 240 r / min, and stir evenly to obtain a viscous solution; S6. Put the bactericidal and antiviral natural extract prepared by S1 and the high-efficiency bactericidal nanoparticles prepared by S4 into the viscous solution prepared by S5, stir at a temperature of 36°C, for 18 min, at a speed of 280 r / min, and stir to mix to obtain a high-efficiency bactericidal suspension.

[0049] In S1, the solid-liquid ratio of the Herba Euphorbiae Ichthyophthirius and the 50% ethanol solution is 1.5:1 g / mL, and in S2, the mass fraction of the potassium diformate in ultrapure water is 39%.

[0050] In S3, the solid-liquid ratio of the citrus fiber powder to N-methylpyrrolidone is 0.2:1 g / mL, in S4, the mass fraction of the potassium hydroxide in ultrapure water is 42%, and in S5, the mass fraction of the potassium alginate in ultrapure water is 4%.

[0051] The method for preparing the disease prevention and resistance enhancement composition specifically comprises the following steps: L1. Put salicylic acid, magnesium chloride, α-mannosidase and 50% ethanol solution into a 1.5 kW stirrer, stir at 28°C, stir for 15 min, stir at 220 r / min, mix and dissolve to obtain a disease resistance enhancement preparation; L2, put polydimethylsiloxane and propylene glycol into a high-speed stirrer with a power of 2.5 kW, the high-speed stirring temperature is 30°C, the high-speed stirring speed is 8000 r / min, the high-speed stirring time is 30 min, and high-speed stirring emulsifies to obtain a breathable emulsion; L3, putting oxidized starch, glutaraldehyde and ultrapure water into a reactor with a power of 2.9 kW, reacting at a temperature of 50°C, a reaction speed of 200 r / min, and a reaction time of 2.3 h to obtain a microporous foaming material; L4, put the microporous foam material prepared by L3 into the disease resistance enhancement preparation prepared by L1, stir at 28°C, stir for 18 min, stir at 250 r / min, mix, put into a freeze dryer with a power of 2.2 kW, freeze dry at a pressure of 300 Pa, and a reduced pressure drying temperature of -45°C, freeze dry for 12 h, heat to -25°C and freeze dry for 8 h, and freeze dry at 15°C for 5 h to obtain a disease prevention and resistance enhancement powder; L5. Put the disease-preventing and resistance-enhancing powder prepared by L4 into the breathable emulsion prepared by L2, and put it into an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min, and perform ultrasonic dispersion to obtain a disease-preventing and resistance-enhancing composition.

[0052] In L1, the solid-liquid ratio of salicylic acid to 50% ethanol solution is 0.15:1 g / mL.

[0053] In L3, the mass fraction of glutaraldehyde in ultrapure water is 28%.

[0054] This embodiment also provides a method for preparing a winter cabbage fungicide, which specifically comprises the following steps: Step 1: put methionine, methyl gallate, p-chlorophenoxyacetic acid, allantoin and 70% ethanol solution into a stirring tank with a power of 1.8 kW, the stirring temperature is 80° C., the stirring time is 19 min, the stirring speed is 240 r / min, and the mixture is mixed to obtain a mixture that promotes plant growth; Step 2: Put the high-efficiency bactericidal suspension and the disease prevention and resistance enhancement composition into a stirrer with a power of 2.1 kW, stir at a temperature of 38° C., stir for 30 min, and stir at a speed of 400 r / min to mix and obtain a high-efficiency bactericidal component; Step 3: Add the plant growth promoting mixture prepared in step 1 to the high-efficiency bactericidal component prepared in step 2, stir at a temperature of 35°C, stir for 26 min, stir at a speed of 400 r / min, mix evenly, and obtain a winter cabbage bactericide.

[0055] In step 1, the solid-liquid ratio of methionine to 70% ethanol solution is 0.13:1 g / mL.

[0056] Comparative Example 1: This comparative example provides a cabbage fungicide and a preparation method thereof, which is different from Example 1 only in that the added high-efficiency fungicidal suspension does not contain high-efficiency fungicidal nanoparticles, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0057] Comparative Example 2: This comparative example provides a cabbage fungicide and a preparation method thereof. The only difference from Example 1 is that the added disease prevention and resistance enhancing composition does not contain disease prevention and resistance enhancing powder, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0058] Experimental Example 1: Test for determination of control ability and long-term control effect.

[0059] The test steps for determining the control ability and long-term control effect of the winter cabbage fungicide prepared in Examples 1-3 of the present invention are as follows: (1) A 6-mu test greenhouse experimental field was opened, the temperature in the greenhouse was 5°C, the humidity was 70%, and the light was natural light. The field was divided into a 3-mu embodiment group, a 2-mu comparative group, and a 1-mu control group. The 3-mu embodiment group cabbage field was respectively applied with the winter cabbage fungicide prepared in Examples 1-3, the 2-mu comparative group cabbage field was respectively applied with the cabbage fungicide prepared in Comparative Examples 1-2, and the control group cabbage field was applied with 75% thiophanate-methyl wettable powder (purchased from Shandong Bainong Sida Biotechnology Co., Ltd.). Three parallel plots were set up, and the application amount was 130 g / mu. Transplanted Lubai No. 1 cabbage seedlings with a plant height of 6 cm were transplanted and planted at an equal distance of 16×22 cm per mu. After planting for 8 days, the winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% thiophanate-methyl wettable powder of the control group were mixed with ultrapure water in a weight ratio of 1:600, dispersed evenly, and applied every 10 A second additional application was performed after d; (2) After the second application, the winter cabbage fungicide prepared in Example 1-3, the cabbage fungicide prepared in Comparative Example 1-2, and the 75% thiophanate-methyl wettable powder of the control group were stopped from being applied to the Lubai No. 1 cabbage in the 6 mu test greenhouse experimental field, and the control effects on downy mildew, soft rot, and mosaic disease of cabbage were recorded on the 10th, 20th, and 30th days after the application was stopped.

[0060] Result analysis: Figure 1 This is a scanning electron microscope image of the internal structure of the highly effective bactericidal nanoparticles described in Experimental Example 1 of the present invention. Figure 2 This is a result diagram of the control effect of downy mildew on Chinese cabbage described in Experimental Example 1 of the present invention. As shown in the figure, the winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% thiophanate-methyl wettable powder of the control group were applied in an amount of 130 g / mu, mixed with ultrapure water at a weight ratio of 1:600, and evenly dispersed. Then, they were applied to the transplanted Lubai No. 1 cabbage seedlings with a plant height of 6 cm. After 10 days, the second additional application was carried out. On the 10th day after stopping the application, the control effect of downy mildew on Chinese cabbage in the cabbage fields of the Example 1-3 groups was 92.8%, 94.4%, and 96.7%, respectively. In the cabbage fields of the Comparative Examples 1-2 groups, the control effect of downy mildew on Chinese cabbage was 74.3% and 90.1%, respectively. In the cabbage fields of the control group, the control effect of downy mildew on Chinese cabbage was 82.5%, and on the 30th day after stopping the application, the control effect of downy mildew on Chinese cabbage was 82.5%. d. In the cabbage fields of Example 1-3, the control effects of downy mildew on Chinese cabbage were 91.7%, 93.7% and 94.8% respectively. In the cabbage fields of Comparative Example 1-2, the control effects of downy mildew on Chinese cabbage were 67.5% and 74.5% respectively. In the cabbage fields of the control group, the control effect of downy mildew on Chinese cabbage was 43.8%. Figure 3The result diagram of the control effect of soft rot of Chinese cabbage described in Experimental Example 1 of the present invention is shown in the figure. The winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% thiophanate-methyl wettable powder of the control group were applied in an amount of 130 g / mu, respectively, and mixed with ultrapure water at a weight ratio of 1:600. After uniform dispersion, they were applied to the transplanted Lubai No. 1 cabbage seedlings with a plant height of 6 cm. A second additional application was performed after 10 days. On the 10th day after stopping the application, the control effect of soft rot of Chinese cabbage in the cabbage fields of Example 1-3 groups was 93.1%, 94.8%, and 96.7%, respectively. In the cabbage fields of Comparative Examples 1-2 groups, the control effect of soft rot of Chinese cabbage was 64.4% and 91.2%, respectively. In the cabbage fields of the control group, the control effect of soft rot of Chinese cabbage was 72.1%, and on the 30th day after stopping the application d. In the cabbage fields of Example 1-3, the control effects of Chinese cabbage soft rot were 92.5%, 94.1%, and 96.2%, respectively. In the cabbage fields of Comparative Example 1-2, the control effects of Chinese cabbage soft rot were 63.1% and 77.1%, respectively. In the cabbage fields of the control group, the control effect of Chinese cabbage soft rot was 49.2%. Figure 4 The result diagram of the control effect of Chinese cabbage mosaic disease described in Experimental Example 1 of the present invention is shown in the figure. As shown in the figure, the winter cabbage fungicide prepared in Example 1-3, the cabbage fungicide prepared in Comparative Example 1-2, and the 75% thiophanate-methyl wettable powder of the control group were applied in an amount of 130 g / mu, mixed with ultrapure water at a weight ratio of 1:600, and evenly dispersed. Then, they were applied to the transplanted Lubai No. 1 cabbage seedlings with a plant height of 6 cm. After 10 days, the second additional application was carried out. On the 10th day after stopping the application, the control effect of Chinese cabbage mosaic disease in the cabbage fields of Example 1-3 groups was 94.3%, 95.7%, and 97.8%, respectively. In the cabbage fields of Comparative Example 1-2 groups, the control effect of Chinese cabbage mosaic disease was 72.6% and 90.9%, respectively. In the cabbage fields of the control group, the control effect of Chinese cabbage mosaic disease was 11.3%, and on the 30th day after stopping the application, d. In the cabbage fields of Example 1-3, the control effects of Chinese cabbage mosaic disease were 93.6%, 94.5% and 96.6% respectively; in the cabbage fields of Comparative Example 1-2, the control effects of Chinese cabbage mosaic disease were 71.3% and 77.0% respectively; in the cabbage fields of the control group, the control effect of Chinese cabbage mosaic disease was 2.7%, indicating that the winter cabbage fungicide prepared by the present invention can effectively control winter cabbage diseases caused by various pathogenic microorganisms, maintain the fungicide effect for a long time, and effectively enhance the plant's own disease resistance. Experimental Example 2: Test to determine the influence of soil pH and microbial colony.

[0061] (1) A 6-mu test greenhouse experimental field was opened, the temperature in the greenhouse was 5°C, the humidity was 70%, and the light was natural light. The field was divided into a 3-mu embodiment group, a 2-mu comparative group, and a 1-mu control group. The 3-mu embodiment group cabbage field was respectively applied with the winter cabbage fungicide prepared in Examples 1-3, the 2-mu comparative group cabbage field was respectively applied with the cabbage fungicide prepared in Comparative Examples 1-2, and the control group cabbage field was applied with 75% thiophanate-methyl wettable powder (purchased from Shandong Bainong Sida Biotechnology Co., Ltd.). Three parallel plots were set up, and the application amount was 130 g / mu. Transplanted Lubai No. 1 cabbage seedlings with a plant height of 6 cm were transplanted and planted at an equal distance of 16×22 cm per mu. After planting for 8 days, the winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% thiophanate-methyl wettable powder of the control group were mixed with ultrapure water in a weight ratio of 1:600, dispersed evenly, and applied every 10 The second additional application was carried out 1 day later, and a total of 7 applications were carried out. Every 10 days after the application, 20 g of soil from each group of experimental fields was taken for pH measurement. The measurement was continued for 70 days and the results were recorded. (2) After 70 days, 10 g of soil was collected from 3 mu of cabbage field in the embodiment group, 2 mu of cabbage field in the comparative group, and 1 mu of cabbage field in the control group, with five replicates in each group; (3) 3 mu of soil from the embodiment group, 2 mu of soil from the comparative group, and 1 mu of soil from the control group were placed in test tubes containing 1 L of sterile liquid LB culture medium, and cultured in a shaking incubator at 37°C and 120 r / min for 24 h to obtain cultured bacterial liquid; (4) Dilute the cultured bacterial solution 100 times. In an ultra-clean workbench, use a pipette to take 50 μL of the cultured bacterial solution and evenly spread it on the LB solid culture medium plate. Then, invert it and culture it in a 37°C incubator for 24 h to obtain the number of bacterial colonies in the experimental group. Calculate the number of bacterial colonies according to the formula: Total bacterial colony count (cfu / g) = number of colonies on each plate × total dilution factor.

[0062] Result analysis: Figure 5 The soil pH value measurement result diagram of Experimental Example 2 of the present invention is shown in the figure. The winter cabbage fungicide prepared in Examples 1-3, the cabbage fungicide prepared in Comparative Examples 1-2, and the 75% thiophanate-methyl wettable powder of the control group were applied in an amount of 130 g / mu, respectively, and mixed with ultrapure water at a weight ratio of 1:600. After uniform dispersion, they were applied once every 10 days for a total of 7 times. After the first application, 20 g of soil was taken every 10 days for pH measurement. After 70 days, the soil pH values ​​of the cabbage fields of the 3 mu embodiment group were 6.3, 6.3, and 6.5, respectively, the soil pH values ​​of the cabbage fields of the 2 mu comparative group were 6.3 and 6.1, respectively, and the soil pH value of the cabbage fields of the control group was 5.6; Figure 6The results of the determination of the bacterial colony count in the soil described in Experimental Example 2 of the present invention are shown in the figure. The winter cabbage fungicide prepared in Example 1-3, the cabbage fungicide prepared in Comparative Example 1-2, and the 75% thiophanate-methyl wettable powder of the control group were applied at an amount of 130 g / mu, respectively, and mixed with ultrapure water at a weight ratio of 1:600. After uniform dispersion, they were applied once every 10 days for a total of 7 times. After 70 days, the bacterial colony count in the soil of the 3-mu cabbage field of the embodiment group was 9.03×10 8 cfu / g, 9.34×10 8 cfu / g, 9.54×10 8 cfu / g, and the bacterial colony counts in the 2-mu cabbage field soil of the control group were 8.96×10 8 cfu / g, 8.93×10 8 cfu / g, and the bacterial colony count in the cabbage field soil of the control group was 6.72×10 8 cfu / g, indicating that the winter cabbage fungicide prepared by the present invention will not cause soil acidification and inactivation of microorganisms in the soil, will not cause soil desertification, has biocompatibility and does not destroy the ecological balance.

[0063] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0064] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A winter cabbage fungicide, characterized in that The winter cabbage fungicide specifically comprises the following components in parts by weight: 70-90 parts of high-efficiency bactericidal suspension, 30-40 parts of disease prevention and resistance enhancement composition, 4-8 parts of methionine, 3-7 parts of methyl gallate, 6-9 parts of p-chlorophenoxyacetic acid, and 2-5 parts of allantoin; The raw materials for preparing the high-efficiency bactericidal suspension include the following components in parts by weight: 11-13 parts of Herba Euphorbiae Ichthyophthirius, 7-10 parts of Rhizoma Cyrthospermi, 8-12 parts of Viola yedoensis, 4-7 parts of Phyllanthus urinariae, 9-13 parts of potassium diformate, 26-33 parts of citrus fiber powder, 11-14 parts of citric acid, 1.8-2.6 parts of trisodium citrate, 8-12 parts of potassium hydroxide, and 12-16 parts of potassium alginate; The raw materials for preparing the disease prevention and resistance enhancement composition include the following components in parts by weight: 2-4 parts of salicylic acid, 3-5 parts of magnesium chloride, 3-6 parts of α-mannosidase, 17-24 parts of polydimethylsiloxane, 8-11 parts of propylene glycol, 25-31 parts of oxidized starch, and 13-16 parts of glutaraldehyde.

2. The winter cabbage fungicide according to claim 1, characterized in that The preparation method of the highly effective bactericidal suspension specifically comprises the following steps: S1. Grind Herba Euphorbiae Ichthyophthirius, Rhizoma Cynomorii, Viola yedoensis, and Phyllanthus urinariae, extract with 50% ethanol solution by ultrasonic wave, and concentrate to obtain a bactericidal and antiviral natural extract; S2, dissolving potassium diformate in ultrapure water to obtain a potassium diformate solution; S3, mixing the citrus fiber powder with the potassium diformate solution, citric acid, trisodium citrate and N-methylpyrrolidone prepared in S2, and reacting the mixture to obtain a porous matrix; S4, dissolving potassium hydroxide in ultrapure water, adding the porous matrix prepared in S3, stirring for full adsorption, and freeze spray drying to obtain highly efficient bactericidal nanoparticles; S5, mixing potassium alginate and ultrapure water, stirring evenly to obtain a viscous solution; S6. Put the bactericidal and antiviral natural extract prepared in S1 and the highly effective bactericidal nanoparticles prepared in S4 into the viscous solution prepared in S5, stir and mix, and obtain a highly effective bactericidal suspension.

3. The winter cabbage fungicide according to claim 2, characterized in that In S1, the solid-liquid ratio of the Herba Euphorbiae Ichthyophthirius and the 50% ethanol solution is 1.5:1 g / mL, and in S2, the mass fraction of the potassium diformate in ultrapure water is 35-39%.

4. The winter cabbage fungicide according to claim 3, characterized in that In S3, the solid-liquid ratio of the citrus fiber powder to N-methylpyrrolidone is 0.2:1 g / mL, in S4, the mass fraction of the potassium hydroxide in ultrapure water is 36-42%, and in S5, the mass fraction of the potassium alginate in ultrapure water is 2-4%.

5. The winter cabbage fungicide according to claim 4, characterized in that The method for preparing the disease prevention and resistance enhancement composition specifically comprises the following steps: L1. Mix and dissolve salicylic acid, magnesium chloride, α-mannosidase and 50% ethanol solution to obtain a disease resistance enhancing preparation; L2, emulsifying polydimethylsiloxane and propylene glycol by high-speed stirring to obtain a breathable emulsion; L3, mixing oxidized starch with glutaraldehyde and ultrapure water to react to obtain a microporous foaming material; L4, placing the microporous foaming material prepared in L3 into the disease resistance enhancing preparation prepared in L1, mixing, and freeze-drying to obtain a disease prevention and resistance enhancing powder; L5. Put the disease-preventing and resistance-enhancing powder prepared in L4 into the breathable emulsion prepared in L2, and perform ultrasonic dispersion to obtain a disease-preventing and resistance-enhancing composition.

6. The winter cabbage fungicide according to claim 5, characterized in that In L1, the solid-liquid ratio of salicylic acid to 50% ethanol solution is 0.15:1 g / mL.

7. The winter cabbage fungicide according to claim 6, characterized in that In L3, the mass fraction of glutaraldehyde in ultrapure water is 20-28%.

8. A method for preparing the winter cabbage fungicide according to any one of claims 1 to 7, characterized in that: The specific steps include: Step 1: Mix and dissolve methionine, methyl gallate, p-chlorophenoxyacetic acid, allantoin and 70% ethanol solution to obtain a mixture for promoting plant growth; Step 2: Mixing the high-efficiency bactericidal suspension and the disease prevention and resistance enhancement composition to obtain a high-efficiency bactericidal component; Step 3: Add the plant growth promoting mixture prepared in step 1 to the high-efficiency bactericidal component prepared in step 2, stir evenly, and obtain a winter cabbage bactericide.

9. The method for preparing the winter cabbage fungicide according to claim 8, characterized in that: In step 1, the solid-liquid ratio of methionine to 70% ethanol solution is 0.13:1 g / mL.

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