Black fruit extract composition with effects of reducing blood sugar, blood lipid and blood pressure and preparation method of black fruit extract composition

Abstract

The invention relates to the technical field of preparation and application of natural plant extracts, and discloses a black fruit extract composition with effects of reducing blood sugar, blood lipid and blood pressure and a preparation method of the black fruit extract composition. The composition comprises 65% of aronia melanocarpa, 12% of hawthorn, 8% of lycium barbarum polysaccharide, 5% of green tea, 2% of curcumin, 4% of prebiotic fiber, 1.5% of magnesium element, 3.5% of microcrystalline cellulose, 1.5% of lactose and 0.5% of magnesium stearate. According to the black fruit extract composition with the effects of reducing blood sugar, blood fat and blood pressure and the preparation method of the black fruit extract composition, aronia melanocarpa is used as a main extract, efficient extraction of various bioactive components is achieved, the functions of reducing blood sugar, blood fat and blood pressure of the composition are remarkably enhanced, moisture is removed at low temperature through a vacuum freeze-drying technology, and the effect of reducing blood sugar, blood fat and blood pressure is achieved. The damage of high temperature to heat-sensitive substances is avoided, the materials are uniformly spread and pre-frozen, and the consistency and thoroughness of the drying process are ensured.

Description

Technical Field

[0001] The invention relates to the technical field of preparation and application of natural plant extracts, in particular to a Rutgers rutaecarpa extract composition with blood sugar, lipid and blood pressure lowering effects and a preparation method thereof. Background Art

[0002] In recent years, with the change of people's lifestyle and unreasonable diet structure, the incidence of chronic diseases such as diabetes, hyperlipidemia and hypertension has increased year by year. The prevention and treatment of these diseases has become an important topic in the field of global public health. Natural plant extracts have gradually become a research hotspot due to their rich bioactive ingredients and low toxic side effects. However, most black fruit extract products on the market currently have problems such as low active ingredient extraction rate, low purity, and poor stability, which limit their wide application in the field of functional foods and medicine. Traditional extraction methods mainly include water extraction, alcohol extraction, and solvent extraction. Although these methods are simple and easy to implement, they have obvious deficiencies in extraction efficiency, active ingredient protection, and product quality control. In addition, a single extraction method often cannot fully extract a variety of active ingredients in black fruit, resulting in limited functionality of the extract. Therefore, developing an efficient, comprehensive, and stable method for preparing black fruit extract is of great significance for improving the quality and application value of the extract. Summary of the invention

[0003] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0004] In view of the above existing problems, the present invention is proposed.

[0005] Therefore, the present invention provides a Rudbeckia rutaecarpa extract composition with blood sugar, lipid and blood pressure lowering effects and a preparation method thereof to solve the problems of low active ingredient extraction rate, low purity, poor stability and the like of most Rudbeckia rutaecarpa extract products on the market, which limit their wide application in the fields of functional foods and medicine.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0007] In a first aspect, the present invention provides a composition of black fruit extract having blood sugar, lipid and blood pressure lowering effects, wherein the black fruit extract is aronia nigra; the composition comprises 65% aronia nigra, 12% hawthorn, 8% wolfberry polysaccharide, 5% green tea, 2% curcumin, 4% prebiotic fiber, 1.5% magnesium, 3.5% microcrystalline cellulose, 1.5% lactose and 0.5% magnesium stearate.

[0008] As a preferred embodiment of the black fruit extract composition with hypoglycemic, lipid-lowering and blood pressure-lowering effects of the present invention, the black fruit aronia extract is obtained by an extraction process, which includes using 70% ethanol as an extraction solvent, stirring and extracting at a temperature within a range of 45°C to 55°C for 60 minutes, then using a 0.22μm filter membrane for membrane filtration and C18 reverse phase silica gel column chromatography for purification, and using high performance liquid chromatography to qualitatively and quantitatively analyze anthocyanins and flavonoids in the extract.

[0009] In a second aspect, the present invention provides a method for preparing a black fruit extract composition having the effects of lowering blood sugar, lowering blood lipids and lowering blood pressure, comprising selecting fresh or dried black fruit glandular rib aronia fruits, washing and removing stems, and crushing them into small pieces with a particle size of less than 2 mm to obtain crushed black fruit glandular rib aronia; placing the crushed black fruit glandular rib aronia in a freeze dryer, and vacuum drying at -50°C until the moisture content is less than 5% to obtain black fruit glandular rib aronia powder; adding the black fruit glandular rib aronia powder to an extraction tank filled with 70% ethanol, and evaporating the mixture at a temperature between 45°C and 55°C. The extract is extracted with internal stirring for 60 minutes to obtain a crude extract; the crude extract is filtered through a filter membrane to remove macromolecular impurities, and further purified through C18 reverse phase silica gel column chromatography to collect fractions containing high concentrations of anthocyanins and flavonoids to obtain a purified extract; the purified extract is concentrated under reduced pressure, and then made into a powder form by spray drying or freeze drying technology to obtain an Aronia nigra extract, and the Aronia nigra extract is fully mixed with other synergistic ingredients and excipients using a three-dimensional motion mixer to obtain a final composition.

[0010] As a preferred embodiment of the preparation method of the black fruit extract composition with hypoglycemic, lipid-lowering and blood pressure-lowering effects of the present invention, the method comprises the following steps: selecting fresh or dried Aronia nigra fruits, washing, destemming, and crushing them into small pieces with a particle size of less than 2 mm to obtain crushed Aronia nigra, and the specific steps are: selecting organically grown fresh Aronia nigra fruits as raw materials, using food-grade cleaning liquid to spray-clean the fruits to remove surface impurities and existing pesticide residues, the cleaning time is set to 3 minutes, and the water flow pressure is controlled at 0.3 MPa; and using mechanical destemming equipment to automatically separate the fruit stems to obtain crushed Aronia nigra.

[0011] As a preferred embodiment of the preparation method of the black fruit extract composition with hypoglycemic, lipid-lowering and blood pressure-lowering effects of the present invention, the method comprises the following steps: placing the crushed black fruit glandular ribs Aronia nigra in a freeze dryer, and vacuum drying at -50°C until the moisture content is less than 5% to obtain black fruit glandular ribs Aronia nigra powder, and the specific steps are: using vacuum freeze drying technology to remove moisture on the surface of the crushed black fruit glandular ribs Aronia nigra at low temperature; spreading the crushed black fruit glandular ribs Aronia nigra evenly on a stainless steel tray with a thickness not exceeding 2 cm, placing it in a freeze dryer, and pre-freezing it at -40°C to -50°C for 2 hours to completely freeze the material; drying under relatively low vacuum and slightly high temperature conditions for 6 hours until the final moisture content drops below 5%; after drying, taking out the dried black fruit glandular ribs Aronia nigra powder, and immediately sealing and packaging it.

[0012] As a preferred embodiment of the preparation method of the black fruit extract composition with hypoglycemic, lipid-lowering and blood pressure-lowering effects of the present invention, the black fruit glandular rib aronia powder is added to an extraction tank filled with 70% ethanol, and stirred and extracted for 60 minutes at a temperature range of 45°C to 55°C to obtain a crude extract. The specific steps are: using 70% ethanol as an extraction solvent, and selecting a temperature range of 45°C to 55°C and an extraction time of 60 minutes; accurately weighing the black fruit glandular rib aronia powder, adding it to an extraction tank filled with 70% ethanol, with each gram of powder corresponding to 10mL of extraction solvent; optimizing the extraction efficiency during the extraction process to ensure the quality stability and consistency of the final product, and the expression is:

[0013]

[0014] Where E(t) is the extraction efficiency at time t, C f is the concentration of the target component in the final extract, C i is the target component concentration in the initial powder, k e is the extraction rate constant, and t is the extraction time. After the extraction is completed, the undissolved solid residue is immediately removed by centrifugation or filtration to collect the crude extract containing high concentrations of anthocyanins and flavonoids.

[0015] As a preferred embodiment of the preparation method of the black fruit extract composition with hypoglycemic, lipid-lowering and blood pressure-lowering effects of the present invention, the method comprises: filtering the crude extract with a filter membrane to remove macromolecular impurities, further purifying the crude extract with a C18 reverse-phase silica gel column chromatography, collecting the fraction containing high concentrations of anthocyanins and flavonoids, and obtaining the purified extract. The specific steps are: filtering the crude extract with a 0.22 μm polyethersulfone filter membrane to remove larger particles and insoluble impurities; preparing a C18 reverse-phase silica gel column with a filling height of 20 cm and an inner diameter of 2.5 cm, using 70% ethanol as an eluent, controlling the flow rate at 1 mL / min, loading the crude extract filtered through the filter membrane into the column, and then performing gradient elution; using high performance liquid chromatography to monitor the changes in the components in the eluent, and collecting the fraction containing high concentrations of anthocyanins and flavonoids according to the characteristic peaks of the target components.

[0016] As a preferred embodiment of the preparation method of the black fruit extract composition with hypoglycemic, lipid-lowering and blood pressure-lowering effects of the present invention, the purified extract is concentrated under reduced pressure, and then spray-dried or freeze-dried to obtain a powder to obtain a black fruit glandular rib aronia extract, and the black fruit glandular rib aronia extract is fully mixed with other synergistic ingredients and auxiliary materials using a three-dimensional motion mixer to obtain a final composition. The specific steps are: transferring the purified extract to a rotary evaporator, controlling the vacuum degree to be below 20 mbar in the temperature range of 40°C to 50°C, and concentrating under reduced pressure to remove most of the solvent, and the concentration process lasts for 3 hours until the volume of the extract is reduced to 10%-15% of the initial volume; the concentrated extract is quickly placed in a freeze dryer, The material is pre-frozen at -50°C for 2 hours to completely freeze the material, and then freeze-dried at a high vacuum degree of 0.1 mbar and a temperature range of -40°C to -50°C for 24 hours to ensure that the moisture content drops below 5%, thereby obtaining a dry Aronia nigra extract powder; after drying, 65% Aronia nigra extract, 12% Hawthorn, 8% Lycium barbarum polysaccharide, 5% Green tea, 2% Curcumin, 4% Prebiotic fiber, 1.5% Magnesium, 3.5% Microcrystalline cellulose, 1.5% Lactose and 0.5% Magnesium stearate are weighed; all the ingredients are fully mixed using a three-dimensional motion mixer, the mixing time is 30 minutes, and the rotation speed is set to 25 rpm; after mixing, the mixed powder is sealed and packaged, and sealed using an aluminum foil bag to obtain a composition.

[0017] In a third aspect, the application of a Rutgers rutaecarpa extract composition having blood sugar, lipid and blood pressure lowering effects, the application of the Rutgers rutaecarpa extract in the food field is specifically used to prepare functional foods, including but not limited to health drinks, healthy snacks and nutritional supplements, wherein: the application of the Rutgers rutaecarpa extract in the food field realizes any step of the Rutgers rutaecarpa extract composition having blood sugar, lipid and blood pressure lowering effects and its preparation method as described in the first and second aspects of the present invention.

[0018] In a fourth aspect, the application of a Rutgers rutaecarpa extract composition having blood sugar, lipid and blood pressure lowering effects, the application of the Rutgers rutaecarpa extract in the medical field is specifically used for preparing drugs for treating and preventing diabetes, hyperlipidemia and hypertension, wherein: the application of the Rutgers rutaecarpa extract in the medical field realizes any step of the Rutgers rutaecarpa extract composition having blood sugar, lipid and blood pressure lowering effects and its preparation method as described in the first and second aspects of the present invention.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] By using Aralia nigra as the main extract, efficient extraction of a variety of bioactive ingredients was achieved, and the hypoglycemic, lipid-lowering and blood pressure-lowering functions of the composition were significantly enhanced. By using vacuum freeze-drying technology to remove moisture at low temperature, the damage of high temperature to heat-sensitive substances was avoided. The material was evenly spread and pre-frozen to ensure the consistency and thoroughness of the drying process. Using 70% ethanol as the extraction solvent, stirring extraction was carried out within an appropriate temperature and time range to ensure that the target components were fully dissolved in the solvent. The solvent ratio was accurately weighed and controlled to ensure the stability and repeatability of the extraction process. By using a 0.22μm polyethersulfone filter membrane to remove larger particles and insoluble impurities, and then further separating the target components and impurities through C18 reverse phase silica gel column chromatography, it can effectively adsorb target compounds with less polarity and is suitable for separation in organic solvents. By gradient elution and HPLC monitoring, the high recovery rate and purity of the target components were ensured, providing high-quality raw materials for subsequent applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The present invention is a schematic flow chart of a method for preparing a Rutaceae extract composition having blood sugar, lipid and blood pressure lowering effects. DETAILED DESCRIPTION

[0022] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and understandable, the specific implementation modes of the present invention are described in detail below in conjunction with the accompanying drawings.

[0023] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0024] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0025] Example 1

[0026] See also Figure 1 , which is the first embodiment of the present invention, and provides a black fruit extract composition having the effects of lowering blood sugar, lowering blood lipids and lowering blood pressure, wherein the black fruit extract is Aronia nigra;

[0027] The composition includes 65% of Aronia nigra, 12% of Hawthorn, 8% of Lycium barbarum polysaccharide, 5% of green tea, 2% of curcumin, 4% of prebiotic fiber, 1.5% of magnesium, 3.5% of microcrystalline cellulose, 1.5% of lactose and 0.5% of magnesium stearate;

[0028] Specifically, the Aronia nigra extract is obtained by an extraction process, the process comprising:

[0029] 70% ethanol was used as the extraction solvent, and the extraction was stirred at 45°C to 55°C for 60 minutes, followed by membrane filtration using a 0.22 μm filter membrane and purification using C18 reverse-phase silica gel column chromatography. High performance liquid chromatography was used to qualitatively and quantitatively analyze the anthocyanins and flavonoids in the extract.

[0030] It should be noted that Aronia nigra is the main extract, with a content of up to 65%, which not only ensures that the composition has significant blood sugar, lipid and blood pressure lowering effects, but also enhances the comprehensive health benefits of the overall formula through a reasonable ratio with other synergistic ingredients (such as hawthorn, wolfberry polysaccharides, etc.). The proportion of each ingredient has been verified by a large number of experiments to ensure that while providing multiple health functions, the stability and safety of the product are maintained.

[0031] Example 2

[0032] See also Figure 1 , which is the second embodiment of the present invention, provides a method for preparing a Rutaceae extract composition having blood sugar, lipid and blood pressure lowering effects, comprising:

[0033] S1. Select fresh or dried Aronia nigra fruits, wash and remove stems, and crush them into small pieces with a particle size of less than 2 mm to obtain crushed Aronia nigra fruits;

[0034] Specifically, fresh or dried Aronia nigra fruits are selected, washed and stemmed, and then crushed into small pieces with a particle size of less than 2 mm to obtain crushed Aronia nigra. The specific steps are: fresh Aronia nigra fruits grown organically are selected as raw materials, food-grade cleaning liquid is used to spray-clean the fruits to remove surface impurities and existing pesticide residues, the cleaning time is set to 3 minutes, and the water flow pressure is controlled at 0.3 MPa; mechanical stem removal equipment is used to automatically separate the fruit stems to obtain the crushed Aronia nigra.

[0035] It should be noted that by selecting fresh organically grown Aronia nigra fruits as raw materials and using food-grade cleaning fluid for spray cleaning, the purity and safety of the raw materials are ensured. The cleaning time is set to 3 minutes and the water flow pressure is controlled at 0.3MPa, which effectively removes surface impurities and pesticide residues. The mechanical destemming equipment is used to automatically separate the fruit stalks, which not only improves work efficiency but also reduces the risk of pollution caused by manual operation. The fruits are broken into small pieces with a particle size of less than 2 mm, which increases the contact area between the material and the solvent and significantly improves the subsequent extraction efficiency.

[0036] S2, placing the crushed Aronia nigra in a freeze dryer, and vacuum drying at -50°C until the moisture content is less than 5%, to obtain Aronia nigra powder;

[0037] Specifically, the crushed Aronia nigra is placed in a freeze dryer, and vacuum dried at -50°C until the moisture content is less than 5% to obtain Aronia nigra powder. The specific steps are: using vacuum freeze drying technology to remove moisture on the surface of the crushed Aronia nigra at low temperature; spreading the crushed Aronia nigra evenly on a stainless steel tray with a thickness of no more than 2 cm, placing it in a freeze dryer, and pre-freezing it at -40°C to -50°C for 2 hours to completely freeze the material; drying is carried out under relatively low vacuum and slightly high temperature conditions for 6 hours until the final moisture content drops below 5%; after drying is completed, taking out the dried Aronia nigra powder, and immediately sealing and packaging it.

[0038] It should be noted that vacuum freeze-drying technology is used to remove moisture at low temperature, which maximizes the retention of biologically active ingredients in the fruit, such as anthocyanins and flavonoids. The material is evenly spread and pre-frozen for 2 hours to ensure the consistency and thoroughness of the drying process. Drying is carried out under relatively low vacuum and slightly higher temperature conditions for 6 hours, and the final moisture content drops below 5%. The dried black fruit glandular rib ash powder is immediately sealed and packaged to prevent moisture absorption, thereby ensuring the stability of the product and long-term storage performance.

[0039] S3, adding the Aronia nigra powder into an extraction tank filled with 70% ethanol, stirring and extracting at a temperature between 45° C. and 55° C. for 60 minutes to obtain a crude extract;

[0040] Specifically, the black fruit glandular rib aronia powder is added to an extraction tank filled with 70% ethanol, and the mixture is stirred and extracted at a temperature range of 45° C. to 55° C. for 60 minutes to obtain a crude extract. The specific steps are: using 70% ethanol as an extraction solvent, and selecting a temperature range of 45° C. to 55° C. and an extraction time of 60 minutes; accurately weighing the black fruit glandular rib aronia powder, and adding it to an extraction tank filled with 70% ethanol, with each gram of powder corresponding to 10 mL of extraction solvent; optimizing the extraction efficiency during the extraction process to ensure the quality stability and consistency of the final product, and the expression is:

[0041]

[0042] Where E(t) is the extraction efficiency at time t, C f is the concentration of the target component in the final extract, C i is the target component concentration in the initial powder, k e is the extraction rate constant, and t is the extraction time. After the extraction is completed, the undissolved solid residue is immediately removed by centrifugation or filtration to collect the crude extract containing high concentrations of anthocyanins and flavonoids.

[0043] It should be noted that 70% ethanol was used as the extraction solvent, and the extraction was stirred in the range of 45°C to 55°C for 60 minutes. This condition was based on the study of the optimal solubility of the active ingredients of Aronia nigra. This temperature range can effectively extract the target ingredients without destroying heat-sensitive substances due to excessively high temperatures. The ratio of 10 mL of extraction solvent per gram of powder ensures sufficient infiltration and efficient extraction. The introduction of the extraction efficiency formula can scientifically evaluate and optimize the extraction parameters to ensure the quality stability and consistency of the final product. After the extraction is completed, the undissolved solid residue is immediately removed by centrifugation or filtration, and the crude extract containing high concentrations of anthocyanins and flavonoids is collected.

[0044] S4, filtering the crude extract with a filter membrane to remove macromolecular impurities, further purifying by C18 reverse phase silica gel column chromatography, collecting fractions containing high concentrations of anthocyanins and flavonoids, and obtaining a purified extract;

[0045] Specifically, the crude extract is filtered through a filter membrane to remove macromolecular impurities, and further purified through C18 reverse phase silica gel column chromatography, and fractions containing high concentrations of anthocyanins and flavonoids are collected to obtain a purified extract. The specific steps are: filtering the crude extract through a 0.22 μm polyethersulfone filter membrane to remove larger particles and insoluble impurities; preparing a C18 reverse phase silica gel column with a filling height of 20 cm and an inner diameter of 2.5 cm, using 70% ethanol as an eluent, and controlling the flow rate at 1 mL / min, loading the crude extract filtered through the filter membrane into the column, and then performing gradient elution; using high performance liquid chromatography to monitor changes in the components in the eluent, and collecting fractions containing high concentrations of anthocyanins and flavonoids according to the characteristic peaks of the target components.

[0046] It should be noted that first, a 0.22 μm polyethersulfone filter membrane is used to remove larger particles and insoluble impurities, and then C18 reverse-phase silica gel column chromatography is used to further separate the target components and impurities. High performance liquid chromatography (HPLC) is used to monitor the changes in the components in the eluate, and fractions containing high concentrations of anthocyanins and flavonoids are collected based on characteristic peaks. The selection of the C18 column can effectively adsorb the target compounds with lower polarity and is suitable for separation in organic solvents. Gradient elution and HPLC monitoring ensure high recovery and purity of the target components, providing high-quality raw materials for subsequent applications.

[0047] S5, concentrating the purified extract under reduced pressure, and then preparing it into powder form by spray drying or freeze drying technology to obtain an Aronia nigra extract, and fully mixing the Aronia nigra extract with other synergistic ingredients and auxiliary materials by using a three-dimensional motion mixer to obtain a final composition;

[0048] Specifically, the purified extract is concentrated under reduced pressure, and then made into a powder form by spray drying or freeze drying technology to obtain a black fruit glandular rib aronia extract, and the black fruit glandular rib aronia extract is fully mixed with other synergistic ingredients and auxiliary materials using a three-dimensional motion mixer to obtain a final composition. The specific steps are: transferring the purified extract to a rotary evaporator, controlling the vacuum degree below 20mbar in the temperature range of 40°C to 50°C, and concentrating under reduced pressure to remove most of the solvent. The concentration process lasts for 3 hours until the volume of the extract is reduced to 10%-15% of the initial volume; the concentrated extract is quickly placed in a freeze dryer and pre-frozen at -50°C for 2 hours to completely freeze the material. The mixture was freeze-dried at a high vacuum of 0.1 mbar and a temperature range of -40°C to -50°C for 24 hours to ensure that the moisture content dropped below 5% to obtain a dry powder of Aronia nigra extract. After the drying was completed, 65% of Aronia nigra extract, 12% of Hawthorn, 8% of Lycium barbarum polysaccharides, 5% of green tea, 2% of curcumin, 4% of prebiotic fiber, 1.5% of magnesium, 3.5% of microcrystalline cellulose, 1.5% of lactose and 0.5% of magnesium stearate were weighed. All the ingredients were fully mixed using a three-dimensional motion mixer, the mixing time was 30 minutes, and the speed was set to 25 rpm. After the mixing was completed, the mixed powder was sealed and packaged, and sealed with an aluminum foil bag to obtain a composition.

[0049] It should be noted that the purified extract was transferred to a rotary evaporator, and the vacuum was controlled below 20 mbar in the temperature range of 40°C to 50°C to carry out reduced pressure concentration to remove most of the solvent. The concentration process lasted for 3 hours until the volume of the extract was reduced to 10%-15% of the initial volume. The concentrated extract was quickly placed in a freeze dryer and pre-frozen at -50°C for 2 hours to completely freeze the material. It was then freeze-dried at a high vacuum of 0.1 mbar and a temperature range of -40°C to -50°C for 24 hours to ensure that the moisture content dropped below 5% to obtain a dry black fruit glandular rib aronia extract powder. After drying, the ingredients were weighed and all the ingredients were fully mixed using a three-dimensional motion mixer. The mixing time was 30 minutes and the speed was set to 25 rpm. Finally, the mixed powder was sealed and packaged and sealed with an aluminum foil bag to obtain the final composition. The process ensured uniform distribution of product ingredients and improved product stability and quality.

[0050] The application of black fruit extract in the food field is specifically used to prepare functional foods, including but not limited to health drinks, healthy snacks and nutritional supplements. These functional foods can not only provide the nutrients needed for daily life, but also play the health effects of lowering blood sugar, lowering blood lipids and lowering blood pressure through their unique active ingredients. For example, adding black fruit extract to health drinks can make healthy drinks with antioxidant and anti-inflammatory effects, which are suitable for people who need to control blood sugar and blood lipids. In addition, adding black fruit extract to healthy snacks, such as energy bars or nut mixtures, can provide rich vitamin C, anthocyanins and flavonoids, which help to enhance immunity and improve cardiovascular health. Adding black fruit extract to nutritional supplements can be made into tablets, capsules or powders, which are convenient for consumers to take daily and effectively prevent and assist in the treatment of chronic diseases such as diabetes, hyperlipidemia and hypertension. Through these applications, black fruit extract not only enriches the types of functional foods, but also enhances their health value and meets the needs of modern consumers for healthy foods.

[0051] The application of black fruit extract in the medical field is specifically used to prepare drugs for the treatment and prevention of diabetes, hyperlipidemia and hypertension. These drugs can work through various routes, such as oral, injection or local application, to achieve the best therapeutic effect. For example, black fruit extract is made into oral tablets or capsules, which can effectively lower blood sugar levels and improve insulin sensitivity, and is suitable for diabetic patients. Black fruit extract is made into oral liquid or injection, which can significantly lower blood lipid levels and reduce the risk of atherosclerosis, and is suitable for patients with hyperlipidemia. In addition, black fruit extract is made into sustained-release preparations or patches, which can continuously release active ingredients and effectively lower blood pressure, and is suitable for patients with hypertension. Through these drug forms, the various active ingredients of black fruit extract (anthocyanidins, flavonoids, polysaccharides, vitamin C and organic acids) can fully exert their pharmacological effects, improve therapeutic effects, and reduce side effects. The application not only expands the application scope of black fruit extract in the medical field, but also provides new options and solutions for the treatment of chronic diseases.

[0052] In summary, by using Aralia nigra as the main extract, efficient extraction of a variety of bioactive ingredients was achieved, and the hypoglycemic, lipid-lowering and blood pressure-lowering functions of the composition were significantly enhanced. By using vacuum freeze-drying technology to remove moisture at low temperature, the damage of heat-sensitive substances to high temperature was avoided. The material was evenly spread and pre-frozen to ensure the consistency and thoroughness of the drying process. 70% ethanol was used as the extraction solvent, and the stirring extraction was carried out within an appropriate temperature and time range to ensure that the target components were fully dissolved in the solvent. The solvent ratio was accurately weighed and controlled to ensure the stability and repeatability of the extraction process. Larger particles and insoluble impurities were removed by using a 0.22 μm polyethersulfone filter membrane, and then the target components and impurities were further separated by C18 reverse phase silica gel column chromatography. It can effectively adsorb target compounds with less polarity and is suitable for separation in organic solvents. By gradient elution and HPLC monitoring, the high recovery rate and purity of the target components were ensured, providing high-quality raw materials for subsequent applications.

[0053] Example 3

[0054] Please refer to Table 1, which is the third embodiment of the present invention. This embodiment provides the application effect of the Rutaceae extract composition with hypoglycemic, lipid-lowering and blood pressure-lowering effects in the preparation of fruit juice, as follows:

[0055] In order to verify the application effect of the Aronia nigra extract composition in the preparation of functional juice, a series of rigorous experiments were carried out.

[0056] Firstly, fresh organically grown Aronia nigra fruits were selected as raw materials, and the fruits were spray-cleaned with food-grade cleaning fluid to remove surface impurities and pesticide residues. The cleaning time was set to 3 minutes, and the water flow pressure was controlled at 0.3 MPa. Subsequently, mechanical stem removal equipment was used to automatically separate the fruit stems to obtain crushed Aronia nigra.

[0057] The crushed Aronia nigra is evenly spread on a stainless steel tray with a thickness not exceeding 2 cm, and is placed in a freeze dryer and vacuum dried at -50°C until the moisture content is less than 5% to obtain Aronia nigra powder. Next, the Aronia nigra powder is added to an extraction tank filled with 70% ethanol, and stirred and extracted at 45°C to 55°C for 60 minutes to obtain a crude extract. During the extraction process, each gram of powder corresponds to 10 mL of extraction solvent to ensure sufficient infiltration. By introducing an extraction efficiency formula, the extraction parameters are optimized to ensure the quality stability and consistency of the final product. After the extraction is completed, the undissolved solid residue is immediately removed by centrifugation or filtration to collect the crude extract containing high concentrations of anthocyanins and flavonoids.

[0058] The crude extract is filtered through a 0.22 μm polyethersulfone filter membrane to remove larger particles and insoluble impurities; then it is further purified by C18 reverse phase silica gel column chromatography to collect fractions containing high concentrations of anthocyanins and flavonoids, high performance liquid chromatography (HPLC) is used to monitor changes in the components in the eluent, and target components are collected according to characteristic peaks. The purified extract is transferred to a rotary evaporator, and the vacuum degree is controlled below 20 mbar in the temperature range of 40°C to 50°C for reduced pressure concentration to remove most of the solvent. The concentration process lasts for 3 hours until the volume of the extract is reduced to 10%-15% of the initial volume. The concentrated extract is quickly placed in a freeze dryer and pre-frozen at -50°C for 2 hours to completely freeze the material, and then freeze-dried at a high vacuum degree of 0.1 mbar and a temperature range of -40°C to -50°C for 24 hours to ensure that the moisture content is reduced to below 5%, thereby obtaining a dry black fruit glandular rib aronia extract powder.

[0059] After drying, weigh 65% of Aronia nigra extract, 12% of hawthorn, 8% of wolfberry polysaccharides, 5% of green tea, 2% of curcumin, 4% of prebiotic fiber, 1.5% of magnesium, 3.5% of microcrystalline cellulose, 1.5% of lactose and 0.5% of magnesium stearate, and use a three-dimensional motion mixer to fully mix all the ingredients. The mixing time is 30 minutes and the speed is set to 25 rpm. Finally, the mixed powder is sealed and packaged with an aluminum foil bag to obtain the final composition.

[0060] The composition was added to a basic juice formula in a certain proportion to prepare two different types of juice samples: one was ordinary juice (not containing the black fruit glandular rib extract composition), and the other was functional juice (containing the black fruit glandular rib extract composition). In order to ensure the scientificity and reliability of the experimental results, repeated experiments were carried out many times.

[0061] Tabular data analysis:

[0062]

[0063]

[0064] Table 1 Comparison of main components and properties of ordinary juice and functional juice

[0065] From the data in Table 1, it can be seen that the functional juice added with the composition of Aronia nigra extract showed significant advantages in many aspects:

[0066] The total phenolic content of functional juice reached 120 mg GAE / 100 mL, which was about 140% higher than that of ordinary juice, indicating that it was rich in more natural antioxidants.

[0067] The anthocyanin content of functional juice is 80mg / 100mL, which is much higher than the 10mg / 100mL of ordinary juice. Anthocyanin is one of the strongest known natural antioxidants, which helps delay the aging process and prevent the occurrence and development of chronic diseases.

[0068] The antioxidant capacity of functional juice reaches 350μmol Trolox Eq / mL, which is about 250% higher than that of ordinary juice. It not only improves the nutritional value of the juice, but also enhances its health functions, such as reducing the risk of cardiovascular disease.

[0069] The sugar content of functional juice is 10g / 100mL, which is about 16.7% lower than that of ordinary juice. This feature is especially important for diabetic patients, reducing the risk of excessive sugar intake.

[0070] Example 4

[0071] Please refer to Table 2, which is the fourth embodiment of the present invention. This embodiment provides the application effect of the Rutaceae extract composition with hypoglycemic, lipid-lowering and blood pressure-lowering effects in the preparation of powders, as follows:

[0072] In order to verify the application effect of the Aronia nigra extract composition in the preparation of functional juice, a series of rigorous experiments were carried out.

[0073] The preparation steps are consistent with those in Example 3, and the final functional powder is obtained;

[0074] The functional powder was added to the basic nutritional formula in a certain proportion to prepare two different types of powder samples: one was ordinary nutritional powder (not containing the black fruit glandular-ribbed Aronia serrata extract composition), and the other was functional nutritional powder (containing the black fruit glandular-ribbed Aronia serrata extract composition). In order to ensure the scientificity and reliability of the experimental results, repeated experiments were carried out many times.

[0075] Tabular data analysis

[0076] Parameter name unit Ordinary nutritional powder Functional nutritional powder Total phenol content mg GAE / 100g 50 120 Anthocyanin content mg / 100g 10 80 Antioxidant Capacity μmol Trolox Eq / g 100 350 Solubility ％ 90 95 Sugar Content g / 100g 12 10 Fat Content g / 100g 0.5 0.3 Sodium Content mg / 100g 15 10

[0077] Table 2 Comparison of main ingredients and properties of common nutritional powder and functional nutritional powder

[0078] From the data in Table 2, it can be seen that the functional nutritional powder with the Sorbus nigra extract composition exhibits significant advantages in many aspects:

[0079] The total phenol content of the functional health care tablets reached 80 mg GAE / tablet, which was about 220% higher than that of ordinary health care tablets, indicating that it was richer in natural antioxidants.

[0080] The anthocyanin content of functional health care tablets is 50 mg / tablet, which is much higher than the 5 mg / tablet of ordinary health care tablets. Anthocyanin is one of the strongest known natural antioxidants, which helps to delay the aging process and prevent the occurrence and development of chronic diseases.

[0081] The antioxidant capacity of the functional health care tablets reaches 200μmol Trolox Eq / tablet, which is about 300% higher than that of ordinary health care tablets. It not only improves the nutritional value of the tablets, but also enhances their health care functions, such as reducing the risk of cardiovascular diseases.

[0082] The disintegration time of functional health care tablets is 90 seconds, which is 30 seconds shorter than that of ordinary health care tablets. This feature enables functional health care tablets to release active ingredients faster after taking them and improve absorption efficiency.

[0083] Example 5

[0084] Please refer to Table 3, which is the fifth embodiment of the present invention. This embodiment provides the application effect of the Rutaceae extract composition with hypoglycemic, lipid-lowering and blood pressure-lowering effects in the preparation of tablets, as follows:

[0085] In order to verify the application effect of the Aronia melanocarpa extract composition in the preparation of functional tablets, detailed experiments were carried out.

[0086] The preparation steps are consistent with those of Example 3. Finally, the mixed powder is pressed into tablets. Each tablet weighs 500 mg, and the content of Aronia melanocarpa extract is 325 mg.

[0087] The functional tablets were added to the basic health care product formula in a certain proportion to prepare two different types of tablet samples: one was an ordinary health care product tablet (not containing the black fruit glandular ribs extract composition), and the other was a functional health care product tablet (containing the black fruit glandular ribs extract composition). In order to ensure the scientificity and reliability of the experimental results, repeated experiments were carried out many times.

[0088] Tabular data analysis:

[0089] Parameter name unit Ordinary health supplement tablets Functional health supplement tablets Total phenol content mg GAE / tablet 25 80 Anthocyanin content mg / tablet 5 50 Antioxidant Capacity μmol Trolox Eq / tablet 50 200 Disintegration time Second 120 90 hardness N 100 120 Sugar Content mg / tablet 60 50 Fat Content mg / tablet 25 15 Sodium Content mg / tablet 7.5 5

[0090] Table 3 Comparison of main ingredients and properties of common health care tablets and functional health care tablets

[0091] From the data in Table 3, it can be seen that the functional health care tablets containing the Sorbus nigra extract composition have shown significant advantages in many aspects:

[0092] The total phenol content of the functional health care tablets reached 80 mg GAE / tablet, which was about 220% higher than that of ordinary health care tablets, indicating that it was richer in natural antioxidants.

[0093] The anthocyanin content of functional health care tablets is 50 mg / tablet, which is much higher than the 5 mg / tablet of ordinary health care tablets. Anthocyanin is one of the strongest known natural antioxidants, which helps to delay the aging process and prevent the occurrence and development of chronic diseases.

[0094] The hardness of functional health care tablets is 120N, which is 20% higher than that of ordinary health care tablets. The higher hardness means that the tablets are less likely to break during transportation and storage, thus ensuring the integrity of the product.

[0095] The fat content of energy health tablets is 15 mg / tablet, which is 40% lower than that of ordinary health tablets. It is beneficial for controlling blood lipid levels and supporting cardiovascular health.

[0096] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A composition of Rutaceae extract having blood sugar, lipid and blood pressure lowering effects, characterized in that: The black fruit extract is Aronia nigra; The composition includes 65% of Aronia nigra, 12% of Hawthorn, 8% of Lycium barbarum polysaccharide, 5% of green tea, 2% of curcumin, 4% of prebiotic fiber, 1.5% of magnesium, 3.5% of microcrystalline cellulose, 1.5% of lactose and 0.5% of magnesium stearate.

2. The Rutaceae extract composition having blood sugar, lipid and blood pressure lowering effects according to claim 1, characterized in that: The Aronia nigra extract is obtained by an extraction process, which comprises: 70% ethanol was used as the extraction solvent, and the extraction was stirred at 45°C to 55°C for 60 minutes, followed by membrane filtration using a 0.22 μm filter membrane and purification using C18 reverse-phase silica gel column chromatography. High performance liquid chromatography was used to qualitatively and quantitatively analyze the anthocyanins and flavonoids in the extract.

3. A method for preparing a Rudbeckia rutaecarpa extract composition having blood sugar, lipid and blood pressure lowering effects, according to the Rudbeckia rutaecarpa extract composition having blood sugar, lipid and blood pressure lowering effects according to any one of claims 1-2, characterized in that: The preparation method thereof comprises: Select fresh or dried Aronia nigra fruits, wash and remove stems, and crush them into small pieces with a particle size of less than 2 mm to obtain crushed Aronia nigra; The crushed Aronia nigra was placed in a freeze dryer and vacuum dried at -50°C until the moisture content was less than 5%, thereby obtaining Aronia nigra powder; The powder of Aronia nigra is added into an extraction tank filled with 70% ethanol, and the mixture is stirred and extracted at a temperature between 45° C. and 55° C. for 60 minutes to obtain a crude extract. The crude extract is filtered through a filter membrane to remove macromolecular impurities, and further purified by C18 reverse phase silica gel column chromatography to collect fractions containing high concentrations of anthocyanins and flavonoids to obtain a purified extract; The purified extract is concentrated under reduced pressure, and then made into powder form by spray drying or freeze drying technology to obtain the black fruit glandular rib aronia extract. The black fruit glandular rib aronia extract is fully and evenly mixed with other synergistic ingredients and auxiliary materials by using a three-dimensional motion mixer to obtain a final composition.

4. The Rutaceae extract composition having blood sugar, lipid and blood pressure lowering effects and its preparation method according to claim 3, characterized in that: The method comprises selecting fresh or dried Aronia nigra fruits, washing, removing stems, and crushing them into small pieces with a particle size of less than 2 mm to obtain crushed Aronia nigra, and the specific steps are: Select organically grown fresh Aronia nigra fruits as raw materials, and use food-grade cleaning liquid to spray clean the fruits to remove surface impurities and pesticide residues. The cleaning time is set to 3 minutes, and the water flow pressure is controlled at 0.3MPa. The fruit stems are automatically separated by mechanical stem removal equipment to obtain crushed black fruited Aronia serrata.

5. The Rutaceae extract composition having blood sugar, lipid and blood pressure lowering effects and its preparation method according to claim 3, characterized in that: The crushed Aronia nigra is placed in a freeze dryer and vacuum dried at -50°C until the moisture content is less than 5% to obtain Aronia nigra powder. The specific steps are: The vacuum freeze drying technology was used to remove the moisture on the surface of the crushed Aronia nigra at low temperature. Spread the crushed Aronia nigra evenly on a stainless steel tray with a thickness of no more than 2 cm, put it into a freeze dryer, and pre-freeze it at -40℃ to -50℃ for 2 hours to completely freeze the material; Drying was carried out under lower vacuum and slightly higher temperature conditions for 6 hours until the final moisture content dropped below 5%; After drying is completed, take out the dried Aronia nigra powder and immediately seal it for packaging.

6. The Rutaceae extract composition having blood sugar, lipid and blood pressure lowering effects and its preparation method according to claim 3, characterized in that: The aronia powder is added into an extraction tank filled with 70% ethanol, and the mixture is stirred and extracted at a temperature between 45° C. and 55° C. for 60 minutes to obtain a crude extract. The specific steps are: Using 70% ethanol as the extraction solvent, while selecting a temperature range of 45°C to 55°C and an extraction time of 60 minutes; After accurately weighing the Aronia nigromaculata powder, add it into an extraction tank filled with 70% ethanol, with each gram of powder corresponding to 10 mL of extraction solvent; The extraction efficiency is optimized during the extraction process to ensure the quality stability and consistency of the final product. The expression is: Where E(t) is the extraction efficiency at time t, C f is the concentration of the target component in the final extract, C i is the target component concentration in the initial powder, k e is the extraction rate constant, t is the extraction time; After the extraction is completed, the undissolved solid residue is immediately removed by centrifugation or filtration to collect the crude extract containing high concentrations of anthocyanins and flavonoids.

7. The Rutaceae extract composition having blood sugar, lipid and blood pressure lowering effects and its preparation method according to claim 3, characterized in that: The crude extract is filtered using a filter membrane to remove macromolecular impurities, and further purified by C18 reverse phase silica gel column chromatography to collect fractions containing high concentrations of anthocyanins and flavonoids to obtain a purified extract. The specific steps are: The crude extract was filtered through a 0.22 μm polyethersulfone filter membrane to remove larger particles and insoluble impurities; Prepare a C18 reverse phase silica gel column with a filling height of 20 cm and an inner diameter of 2.5 cm, use 70% ethanol as the eluent, control the flow rate at 1 mL / min, load the crude extract filtered through the filter membrane into the column, and then perform gradient elution; The composition changes in the eluate were monitored using HPLC, and fractions containing high concentrations of anthocyanins and flavonoids were collected based on the characteristic peaks of the target components.

8. The Rutaceae extract composition having blood sugar, lipid and blood pressure lowering effects and its preparation method according to claim 3, characterized in that: The purified extract is concentrated under reduced pressure, and then made into powder form by spray drying or freeze drying technology to obtain the black fruit glandular rib extract, and the black fruit glandular rib extract is fully mixed with other synergistic ingredients and auxiliary materials by a three-dimensional motion mixer to obtain a final composition. The specific steps are: The purified extract was transferred to a rotary evaporator and concentrated under reduced pressure at a temperature range of 40°C to 50°C and a vacuum degree of less than 20 mbar to remove most of the solvent. The concentration process lasted for 3 hours until the volume of the extract was reduced to 10%-15% of the initial volume; The concentrated extract is quickly placed in a freeze dryer, pre-frozen at -50°C for 2 hours to completely freeze the material, and then freeze-dried at a high vacuum degree of 0.1 mbar and a temperature range of -40°C to -50°C for 24 hours to ensure that the moisture content is reduced to less than 5%, thereby obtaining a dry Aronia nigra extract powder; After drying, weigh 65% of Aronia nigra extract, 12% of hawthorn, 8% of Lycium barbarum polysaccharide, 5% of green tea, 2% of curcumin, 4% of prebiotic fiber, 1.5% of magnesium, 3.5% of microcrystalline cellulose, 1.5% of lactose and 0.5% of magnesium stearate; Use a three-dimensional motion mixer to mix all ingredients thoroughly for 30 minutes at a speed of 25 rpm; After the mixing is completed, the mixed powder is sealed and packaged using an aluminum foil bag to obtain a composition.

9. Application of a Rutaceae extract composition having hypoglycemic, lipid-lowering and blood pressure-lowering effects, the Rutaceae extract composition having hypoglycemic, lipid-lowering and blood pressure-lowering effects and a preparation method thereof according to any one of claims 1 to 8, characterized in that: Application of the black fruit extract in the medical field, It is specifically used for preparing medicines for treating and preventing diabetes, hyperlipidemia and hypertension.

10. Application of a Rutaceae extract composition having hypoglycemic, lipid-lowering and blood pressure-lowering effects, the Rutaceae extract composition having hypoglycemic, lipid-lowering and blood pressure-lowering effects and a preparation method thereof according to any one of claims 1 to 8, characterized in that: Application of the black fruit extract in the food field, Specifically used for preparing functional foods, including but not limited to health drinks, healthy snacks and nutritional supplements.

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