Use of microbial compositions in industrial hazardous waste treatment

Abstract

The application discloses application of a microbial composition in industrial hazardous waste treatment and belongs to the technical field of industrial hazardous waste treatment and comprises the following steps: S1, collecting industrial hazardous waste, obtaining hazardous waste materials after pretreatment of the industrial hazardous waste, and filling the hazardous waste materials in a fermentation tank; S2, adding a growth medium in a bioreactor, connecting domesticated bacterial liquid to the bioreactor for expansion culture, and obtaining fermentation materials of compound acidophilic bacteria; S3, adding a biosurfactant to the fermentation materials of the compound acidophilic bacteria, uniformly stirring, and obtaining cell membrane surface hydrophobic modified compound acidophilic bacteria; and S4, then adding an acidic pH buffer solution to obtain a to-be-treated material. The application can maintain the stability of the pH required by the acidophilic bacteria in the process of treating the industrial hazardous waste and improve the cell membrane surface hydrophobicity of the compound acidophilic bacteria.

Description

Technical Field

[0001] This invention relates to the field of industrial hazardous waste treatment technology, specifically the application of microbial compositions in industrial hazardous waste treatment. Background Technology

[0002] The ultimate goal of industrial hazardous waste treatment is harmlessness, volume reduction, and resource recovery. Current methods for industrial hazardous waste treatment include landfill, incineration, pulverization, biological treatment, and various chemical methods. Among these, biological treatment, which utilizes microorganisms to decompose industrial hazardous waste, has long attracted considerable attention from researchers due to its advantages such as low cost, environmental friendliness, and lack of secondary pollution. In the manufacturing processes of industries such as pharmaceuticals, chemicals, and new materials, the generated waste is complex in composition, often containing complex organic compounds and many substances that are difficult to biodegrade, such as various reactor bottom residues, distillation residues after solvent recovery, and waste activated carbon used for adsorption and separation. The treatment of these hazardous wastes is not only technically challenging but also costly, placing a heavy economic and environmental burden on related enterprises.

[0003] Heavy hazardous wastes, such as waste batteries, municipal solid waste incineration fly ash, and degraded catalysts, are highly alkaline solid oxides or hydroxides. Valuable metals in these wastes strongly consume H+ ions during bioleaching, leading to persistently high pH levels in the leachate. This negatively impacts the growth and activity of acidophilic autotrophic bacteria in the leachate. It is evident that under high solid-liquid ratio conditions, the leachate pH is even higher, and the growth and activity of acidophilic autotrophic bacteria are even lower. Therefore, bioleaching technology's capacity to treat heavy hazardous wastes is significantly reduced and treatment costs are significantly increased under high solid-liquid ratios, resulting in low leachating efficiency. Furthermore, highly toxic organic or inorganic substances in heavy hazardous wastes, such as high concentrations of fluoride ions and lithium hexafluorophosphate, can also endanger the growth of the working bacteria, potentially leading to the cessation of the bioleaching process. Due to these factors, the significantly reduced capacity and increased costs of bioleaching technology for treating heavy hazardous wastes under high solid-liquid ratios pose a major obstacle to the practical application of this technology. Summary of the Invention

[0004] In order to overcome the shortcomings of the prior art, one of the objectives of the present invention is to provide the application of microbial compositions in the treatment of industrial hazardous waste, which can maintain the pH stability required by acidophilic thiobacilli during the treatment of industrial hazardous waste and improve the hydrophobicity of the cell membrane surface of the composite acidophilic thiobacilli.

[0005] One of the objectives of this invention is achieved through the following technical solution:

[0006] The application of microbial compositions in the treatment of industrial hazardous waste includes the following steps:

[0007] S1. Collect industrial hazardous waste, pre-treat the industrial hazardous waste to obtain hazardous waste material; fill the hazardous waste material into a fermentation tank;

[0008] S2. The compound acidophilic thiobacillus is subjected to pH adaptation in a culture medium to obtain bacterial cells that can still maintain sulfur oxidation activity under pH conditions of 3.5-5.0. A growth medium containing inorganic salts and a sulfur source is added to a bioreactor. The pH of the growth medium is lowered to 2.0-3.0 using FeSO4·7H2O. The adapted bacterial solution is inoculated into the bioreactor for large-scale culture and cultured for 4-5 days under stirring conditions of 30-50℃ and 120-170rpm. The compound acidophilic thiobacillus enters the logarithmic growth phase and the pH drops below 2.0 to obtain the fermentation material of compound acidophilic thiobacillus.

[0009] S3. Add the biosurfactant to the fermentation material of the composite acidophilic thiobacillus, stir evenly, and obtain the composite acidophilic thiobacillus with hydrophobic modification of cell membrane surface.

[0010] S4. Dilute the hazardous waste material with distilled water to a solid concentration of 10% by mass, and then add an acidic pH buffer solution to obtain the material to be treated;

[0011] S5. The bacterial culture of the hydrophobically modified composite acidophilic thiobacillus obtained in step S3 is inoculated into the fermentation tank. Then, the sulfur source is added as an energy source for cultivation. The culture is rapidly and evenly mixed in the fermentation tank to ensure that the material to be treated can fully and evenly contact and ferment with the hydrophobically modified composite acidophilic thiobacillus. After fermentation, the industrial hazardous waste is effectively treated. Further, the sulfur source includes elemental sulfur or sulfide minerals, and the sulfide minerals include at least one of pyrite, chalcopyrite, and arsenopyrite.

[0012] Further, in step S2, the composite acidophilic thiobacillus is a mixed flora of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, and Leptospirillum ferrooxidans.

[0013] Further, in step S2, the inorganic salt components of the growth medium, by mass concentration, include: 0.5-2.0 g / L KNO3, 0.5-2.0 g / L NaCl, 0.5-2.0 g / L MgSO4·7H2O, 0.25-1.0 g / L CaCl2, with the balance being water.

[0014] Further, in step S2, the acidic pH buffer solution is a potassium chloride hydrochloric acid buffer solution with a pH buffer range of 1.0-2.2.

[0015] Further, the preparation steps of the potassium chloride hydrochloric acid buffer solution according to a volume of 100 mL are as follows: 37.64 mL of 0.1 mol / L HCl and 31.02 mL of 0.02 mol / L KCl solution are mixed and then diluted to 100 mL.

[0016] Furthermore, in step S5, the inoculum concentration is 5%-20% (v / v), the fermentation temperature is 25 to 35°C, and the treatment time is 30 hours.

[0017] Further, in step S3, the preparation method of the biosurfactant is as follows: prepare a growth medium and a fermentation medium for Pseudomonas aeruginosa (ATCC 9027), inoculate the Pseudomonas aeruginosa into the growth medium to obtain a Pseudomonas aeruginosa culture, inoculate the Pseudomonas aeruginosa culture into the fermentation medium to produce the biosurfactant, which is then separated and purified.

[0018] Furthermore, in step S1, the industrial hazardous waste is pretreated by one of the following methods: direct crushing, crushing after separation, or drying and crushing after separation.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0020] This invention provides the application of a microbial composition in the treatment of industrial hazardous waste. In step S4, an acidic pH buffer solution is added to maintain the pH stability required by *Thiobacillus acidophilus* during the treatment of industrial hazardous waste, thus maintaining the growth and activity of *Thiobacillus acidophilus*. Furthermore, the inorganic salts in the culture medium are acidic salts, and *Thiobacillus acidophilus* will also generate hydrogen ions after utilizing the sulfur source, neutralizing the highly alkaline oxides or hydroxides contained in the hazardous waste material and maintaining an acidic environment. In step S3, a biosurfactant is added to the fermentation material of the composite *Thiobacillus acidophilus* and stirred evenly to obtain a composite *Thiobacillus acidophilus* with a hydrophobic modification of the cell membrane surface. The biosurfactant enhances the hydrophobic modification of the cell membrane surface of the composite *Thiobacillus acidophilus*, resulting in a better cell membrane surface. This allows the cell membrane to maintain stability in an acidic environment, preventing disintegration and facilitating the adsorption of *Thiobacillus acidophilus* onto the hazardous waste material, thereby improving the degradation capacity of *Thiobacillus acidophilus* for hazardous waste. Detailed Implementation

[0021] The present invention will be further described below with reference to specific embodiments. It should be noted that, without conflict, the embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0022] Pseudomonas aeruginosa ATCC 9027 was purchased from the Guangdong Institute of Microbiology. The culture was kept in test tubes with solid nutrient agar at 4°C.

[0023] Acid adaptation mechanism of acidophilic bacteria

[0024] Acidophiles thrive in acidic environments, and their cell membranes are their only physical barrier against external H+ ions. Therefore, the low H+ permeability of the cell membrane is likely the primary mechanism by which they maintain their internal pH. Recent studies have shown that lipids on the cell membrane play a crucial role in this process. Research has revealed that liposomes extracted from the cell membranes of these acidophiles cannot form regular vesicle structures at pH 7.0, but can at pH 3.0 and 4.0, exhibiting extremely low H+ permeability. However, these liposome structures become porous at pH 7.0. Thus, acidophiles not only adapt well to acidic environments but also require them to ensure cell membrane stability and cellular integrity.

[0025] Most acidophilic bacteria are chemoautotrophs. Examples of extreme acidophiles include archaea such as *Thiopyroxene* and *Mycoplasma thermophila*, and eubacteria such as *Thiobacillus thiooxidans* and *Thiobacillus ferrooxidans*. *Mycoplasma thermophila* and *Thiopyroxene* are extreme thermophilic acidophilic archaea, found in coal piles and acidic hot springs. Their optimal growth pH is 0.8–3 and 1–5.5, respectively. *Thiobacillus thiooxidans* and *Thiobacillus ferrooxidans* have optimal growth pH of 2–5 and 1.5–4, respectively, and are commonly found in acidic wastewater containing sulfuric acid, coal mines, acidic mineral water, or acidic spring water. *Thiobacillus thiooxidans* and *Thiobacillus ferrooxidans* are Gram-negative, aerobic chemoautotrophs. The ATP and [H] required for CO2 reduction are obtained by oxidizing elemental sulfur to sulfuric acid, simultaneously lowering the environmental pH to 1–1.5. However, their internal pH is 6–6.5, and the optimal pH for their ATPase is 7.5–7.8. Furthermore, *Thiobacillus thiooxidans* cannot oxidize Fe. 2+ Ferrous thiobacillus can oxidize Fe. 2+ . Example 1

[0026] This embodiment provides the application of a microbial composition in the treatment of industrial hazardous waste, including the following steps:

[0027] S1. Collect industrial hazardous waste, and after crushing and pretreatment, obtain hazardous waste material; fill the hazardous waste material into a fermentation tank;

[0028] S2. The compound acidophilic thiobacillus was subjected to pH adaptation in a culture medium to obtain bacterial cells that could still maintain sulfur oxidation activity at pH 3.5. Growth medium containing inorganic salts and sulfur source was added to the bioreactor. The pH of the growth medium was lowered to 2.0 with FeSO4·7H2O. The adapted bacterial solution was inoculated into the bioreactor for scale-up culture and cultured for 4 days at 30℃ and 120rpm. The compound acidophilic thiobacillus entered the logarithmic growth phase and the pH dropped below 2.0, thus obtaining the fermentation material of compound acidophilic thiobacillus.

[0029] S3. Add the biosurfactant to the fermentation material of the compound acidophilic thiobacillus, stir evenly, and obtain the compound acidophilic thiobacillus with hydrophobic modification of cell membrane surface.

[0030] S4. Dilute the hazardous waste material with distilled water to a solid concentration of 10% by mass, and then add an acidic pH buffer solution to obtain the material to be treated.

[0031] S5. The bacterial culture of the composite acidophilic thiobacillus with hydrophobic modification of cell membrane surface obtained in step S3 is inoculated into the fermentation tank at an inoculation concentration of 5% (v / v). Then, a sulfur source is added as an energy substance for cultivation. The culture is rapidly and evenly mixed in the fermentation tank so that the material to be treated can fully and evenly contact the composite acidophilic thiobacillus with hydrophobic modification of cell membrane surface and ferment. The fermentation temperature is 25℃ and the treatment time is 30h. After the fermentation is completed, the industrial hazardous waste is effectively treated.

[0032] The sulfur source in this embodiment can be elemental sulfur or sulfide minerals, including at least one of pyrite, chalcopyrite, and arsenopyrite.

[0033] The complex acidophilic thiobacillus is a mixed flora of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, and Leptospirillum ferrooxidans.

[0034] The inorganic salt composition of the growth medium, by mass concentration, includes: 0.5-2.0 g / L KNO3, 0.5-2.0 g / L NaCl, 0.5-2.0 g / L MgSO4·7H2O, 0.25-1.0 g / L CaCl2, with the balance being water.

[0035] The acidic pH buffer solution is potassium chloride hydrochloride buffer, with a pH buffering range of 1.0-2.2. The preparation steps for a 100 mL volume of potassium chloride hydrochloride buffer are as follows: Mix 37.64 mL of 0.1 mol / L HCl and 31.02 mL of 0.02 mol / L KCl solution, then bring the volume to 100 mL.

[0036] Furthermore, the preparation method of the biosurfactant is as follows: prepare the growth medium and fermentation medium of Pseudomonas aeruginosa ATCC 9027, inoculate Pseudomonas aeruginosa into the growth medium to obtain a Pseudomonas aeruginosa culture, inoculate the Pseudomonas aeruginosa culture into the fermentation medium to produce a biosurfactant, which can be separated and purified.

[0037] The specific preparation steps of the biosurfactant are as follows:

[0038] 1. Preparation of growth medium: by weight percentage, 0.1% beef extract, 0.2% yeast extract, 0.5% heptanone, and 0.5% sodium chloride, pH 7.0. Growth parameters: temperature 28°C, stirring at 150 rpm for 16 hours, until an optical density (OD) of 0.7 at 600 nm (equivalent to 10⁷ CFU / mL) is obtained.

[0039] 2. Preparation of fermentation medium

[0040] By weight percentage: 5.0% glycerol, 2.0% glucose, 0.1% K₂HPO₄, 0.1% K₂HPO₄, 0.02% MgSO₄ 4. 7H2O, 0.02% CaCl 2. The mixture contains H2O, 0.005% FeCl3·6H2O, and has a pH of 7.0. The production parameters are: temperature 28-35℃, stirring at 200 rpm for 96 hours.

[0041] 3. Separation and purification of biosurfactants

[0042] The biosurfactant was extracted by adding ethyl acetate (C4H8O2) at a volume ratio of 1:1 to the fermented broth. The mixture was centrifuged at 5000 rpm for 10 minutes, stirred vigorously for 15 minutes, and allowed to stand until phase separation. The organic phase was removed and evaporated in a rotary evaporator at 40 °C to obtain a viscous yellow product. This product was treated with NaOH and washed with acetone to remove impurities and contaminants to the maximum extent. The yield of the separated biosurfactant was expressed as g / L. Example 2

[0043] This embodiment provides the application of a microbial composition in the treatment of industrial hazardous waste, including the following steps:

[0044] S1. Collect industrial hazardous waste, separate the industrial hazardous waste according to the type of waste, crush the separated waste to obtain hazardous waste material; fill the hazardous waste material into the fermentation tank;

[0045] S2. The compound acidophilic thiobacillus was subjected to pH adaptation in a culture medium to obtain bacterial cells that could maintain sulfur oxidation activity under pH conditions of 3.5–5.0. Growth medium containing inorganic salts and sulfur source was added to the bioreactor. The pH of the growth medium was lowered to 3.0 using FeSO4·7H2O. The adapted bacterial solution was inoculated into the bioreactor for scale-up culture and cultured for 5 days at 40℃ and 150 rpm. The compound acidophilic thiobacillus entered the logarithmic growth phase and the pH dropped below 2.0, thus obtaining the fermentation material of compound acidophilic thiobacillus.

[0046] S3. Add the biosurfactant to the fermentation material of the compound acidophilic thiobacillus, stir evenly, and obtain the compound acidophilic thiobacillus with hydrophobic modification of cell membrane surface.

[0047] S4. Dilute the hazardous waste material with distilled water to a solid concentration of 10% by mass, and then add an acidic pH buffer solution to obtain the material to be treated.

[0048] S5. The bacterial culture of the composite acidophilic thiobacillus with hydrophobic modification of cell membrane surface obtained in step S3 is inoculated into the fermentation tank at an inoculation concentration of 10% (v / v). Then, a sulfur source is added as an energy substance for cultivation. The culture is rapidly and evenly mixed in the fermentation tank so that the material to be treated can fully and evenly contact the composite acidophilic thiobacillus with hydrophobic modification of cell membrane surface and ferment. The fermentation temperature is 30℃ and the treatment time is 30h. After the fermentation is completed, the industrial hazardous waste is effectively treated.

[0049] The sulfur source in this embodiment can be elemental sulfur or sulfide minerals, including at least one of pyrite, chalcopyrite, and arsenopyrite.

[0050] The complex acidophilic thiobacillus is a mixed flora of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, and Leptospirillum ferrooxidans.

[0051] The inorganic salt composition of the growth medium, by mass concentration, includes: 0.5-2.0 g / L KNO3, 0.5-2.0 g / L NaCl, 0.5-2.0 g / L MgSO4·7H2O, 0.25-1.0 g / L CaCl2, with the balance being water.

[0052] The acidic pH buffer solution is potassium chloride hydrochloride buffer, with a pH buffering range of 1.0-2.2. The preparation steps for a 100 mL volume of potassium chloride hydrochloride buffer are as follows: Mix 37.64 mL of 0.1 mol / L HCl and 31.02 mL of 0.02 mol / L KCl solution, then bring the volume to 100 mL.

[0053] Furthermore, the preparation method of the biosurfactant is as follows: prepare the growth medium and fermentation medium of Pseudomonas aeruginosa ATCC 9027, inoculate Pseudomonas aeruginosa into the growth medium to obtain a Pseudomonas aeruginosa culture, inoculate the Pseudomonas aeruginosa culture into the fermentation medium to produce a biosurfactant, which can be separated and purified. Example 3

[0054] This embodiment provides the application of a microbial composition in the treatment of industrial hazardous waste, including the following steps:

[0055] S1. Collect industrial hazardous waste, separate the industrial hazardous waste according to the type of waste, dry the separated waste, and then crush it to obtain hazardous waste material; fill the hazardous waste material into the fermentation tank;

[0056] S2. The compound acidophilic thiobacillus was subjected to pH adaptation in a culture medium to obtain bacterial cells that could still maintain sulfur oxidation activity at pH 5.0. Growth medium containing inorganic salts and sulfur source was added to the bioreactor. The pH of the growth medium was lowered to 2.5 with FeSO4·7H2O. The adapted bacterial solution was inoculated into the bioreactor for large-scale culture and cultured at 50℃ and 170rpm for 4-5 days. The compound acidophilic thiobacillus entered the logarithmic growth phase and the pH dropped below 2.0 to obtain the fermentation material of compound acidophilic thiobacillus.

[0057] S3. Add the biosurfactant to the fermentation material of the compound acidophilic thiobacillus, stir evenly, and obtain the compound acidophilic thiobacillus with hydrophobic modification of cell membrane surface.

[0058] S4. Dilute the hazardous waste material with distilled water to a solid concentration of 10% by mass, and then add an acidic pH buffer solution to obtain the material to be treated.

[0059] S5. The bacterial culture of the hydrophobically modified composite acidophilic thiobacillus obtained in step S3 is inoculated into the fermentation tank at an inoculation concentration of 20% (v / v). Then, a sulfur source is added as an energy source for cultivation. The culture is rapidly and evenly mixed in the fermentation tank so that the material to be treated can fully and evenly contact the hydrophobically modified composite acidophilic thiobacillus and ferment. The fermentation temperature is 35℃ and the treatment time is 30h. After the fermentation is completed, the industrial hazardous waste is effectively treated.

[0060] The sulfur source in this embodiment can be elemental sulfur or sulfide minerals, including at least one of pyrite, chalcopyrite, and arsenopyrite.

[0061] The complex acidophilic thiobacillus is a mixed flora of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, and Leptospirillum ferrooxidans.

[0062] The inorganic salt composition of the growth medium, by mass concentration, includes: 0.5-2.0 g / L KNO3, 0.5-2.0 g / L NaCl, 0.5-2.0 g / L MgSO4·7H2O, 0.25-1.0 g / L CaCl2, with the balance being water.

[0063] The acidic pH buffer solution is potassium chloride hydrochloride buffer, with a pH buffering range of 1.0-2.2. The preparation steps for a 100 mL volume of potassium chloride hydrochloride buffer are as follows: Mix 37.64 mL of 0.1 mol / L HCl and 31.02 mL of 0.02 mol / L KCl solution, then bring the volume to 100 mL.

[0064] Furthermore, the preparation method of the biosurfactant is as follows: prepare the growth medium and fermentation medium of Pseudomonas aeruginosa ATCC 9027, inoculate Pseudomonas aeruginosa into the growth medium to obtain a Pseudomonas aeruginosa culture, inoculate the Pseudomonas aeruginosa culture into the fermentation medium to produce a biosurfactant, which can be separated and purified.

[0065] The above embodiments are merely preferred embodiments of the present invention and should not be construed as limiting the scope of protection of the present invention. Any non-substantial changes and substitutions made by those skilled in the art based on the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. Use of a microbial composition in the treatment of industrial hazardous waste, characterized in that, Includes the following steps: S1. Collect industrial hazardous waste, pre-treat the industrial hazardous waste to obtain hazardous waste material; fill the hazardous waste material into a fermentation tank; S2. The compound acidophilic thiobacillus is subjected to pH adaptation in a culture medium to obtain bacterial cells that can still maintain sulfur oxidation activity under pH conditions of 3.5-5.

0. A growth medium containing inorganic salts and a sulfur source is added to a bioreactor. The pH of the growth medium is lowered to 2.0-3.0 using FeSO4·7H2O. The adapted bacterial solution is inoculated into the bioreactor for large-scale culture and cultured for 4-5 days under stirring conditions of 30-50℃ and 120-170rpm. The compound acidophilic thiobacillus enters the logarithmic growth phase and the pH drops below 2.0 to obtain the fermentation material of compound acidophilic thiobacillus. S3. Add the biosurfactant to the fermentation material of the composite acidophilic thiobacillus, stir evenly, and obtain the composite acidophilic thiobacillus with hydrophobic modification of cell membrane surface. S4. Dilute the hazardous waste material with distilled water to a solid concentration of 10% by mass, and then add an acidic pH buffer solution to obtain the material to be treated; S5. The bacterial solution of the composite acidophilic thiobacillus with hydrophobic modification of cell membrane surface obtained in step S3 is inoculated into the fermentation tank, and then the sulfur source is added as an energy substance for cultivation. The mixture is quickly and evenly mixed in the fermentation tank so that the material to be treated can fully and evenly contact the composite acidophilic thiobacillus with hydrophobic modification of cell membrane surface and ferment. After the fermentation is completed, the industrial hazardous waste is effectively treated. In step S2, the composite acidophilic thiobacillus is a mixed flora of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, and Leptospirillum ferrooxidans. In step S4, the acidic pH buffer solution is a potassium chloride hydrochloric acid buffer solution with a pH range of 1.0 to 2.

2. In step S3, the preparation method of the biosurfactant is as follows: prepare a growth medium and a fermentation medium for Pseudomonas aeruginosa (ATCC 9027), inoculate the Pseudomonas aeruginosa into the growth medium to obtain a Pseudomonas aeruginosa culture, inoculate the Pseudomonas aeruginosa culture into the fermentation medium to produce the biosurfactant, which is then separated and purified.

2. Use of the microbial composition according to claim 1 in the treatment of industrial hazardous waste, characterized in that, In step S2, the sulfur source includes elemental sulfur or sulfide minerals, and the sulfide minerals include at least one of pyrite, chalcopyrite, and arsenopyrite.

3. Use of the microbial composition according to claim 1 in the treatment of industrial hazardous waste, characterized in that, In step S2, the inorganic salt components of the growth medium, by mass concentration, include: 0.5–2.0 g / L KNO3, 0.5–2.0 g / L NaCl, 0.5–2.0 g / L MgSO4·7H2O, 0.25–1.0 g / L CaCl2, with the remainder being water.

4. Use of the microbial composition according to claim 1 in the treatment of industrial hazardous waste, characterized in that, The preparation steps of the potassium chloride hydrochloric acid buffer solution according to a volume of 100 mL are as follows: 37.64 mL of 0.1 mol / L HCl and 31.02 mL of 0.02 mol / L KCl solution are mixed and then the volume is adjusted to 100 mL.

5. Use of the microbial composition according to claim 1 in the treatment of industrial hazardous waste, characterized in that, In step S5, the inoculum concentration is 5%–20% (v / v), the fermentation temperature is 25–35℃, and the treatment time is 30 h.

6. Use of the microbial composition according to claim 1 in the treatment of industrial hazardous waste, characterized in that, In step S1, the industrial hazardous waste is pretreated by one of the following methods: direct crushing, crushing after separation, or drying and crushing after separation.