Protein powder for fodder, and preparing method and use thereof

Abstract

The present invention relates to a preparation method of feed protein powder containing colibacillus yolk antibody whose active valence is greater than or equal to 1:64 and its application. Its preparation method includes the following steps: preparing serotype colibacillus immunogen, using said immunogen to immunize health egg-laying hen, immunogen injection dose is 0.5-3.0 ml / every hen, collecting the egg laid by hen after which is immunized for 7-10 days, remaining the egg containing colibacillus yolk antibody whose active valence is greater than 1:64 for stand-by, using whole egg liquor to make pasteurization and spray-drying.

Description

technical field

[0001] The invention relates to an animal feed raw material, in particular to a feed protein powder containing specific Escherichia coli yolk antibody. The invention also relates to a preparation method and application of the protein powder. Background technique

[0002] Early weaning is an effective means to improve the efficiency of pig farms. However, in actual production, early weaning often leads to "early weaning syndrome" characterized by slow growth after weaning and diarrhea, which seriously affects the performance of piglets. How to recognize and solve weaning stress and help piglets transition to solid feed as soon as possible has always been the focus of attention of pig producers and nutritionists. The most common weaning stress disease in piglets is piglet diarrhoea. Studies have shown that the dietary factor is that allergy to dietary antigens in the intestine leads to damaging changes in the histology and morphological structure of the diges...

Examples

Embodiment 1

[0028] Embodiment 1: the preparation of feed protein powder

[0029] 1. Screening of strains

[0030] Escherichia coli (Escherichia coli) used in the present invention belongs to a kind of in Escherichia Enterobacteriaceae. Anaerobic, glucose-fermenting, lactose-decomposing bacteria. The size of Escherichia coli is 1.1-1.5 μm × 2.0-6.0 μm, with blunt round ends.

[0031] The present invention selects four kinds of Escherichia coli serotypes that are easy to cause diarrhea in weaned piglets after weaning by screening E. coli, namely K88 (China Medical Bacteria Preservation Management Center, strain number is 44742), K99 (China Medical Bacteria Preservation Management Center) Center, strain number 44820), 987P (China Institute of Veterinary Drug Control, strain number 83916) and F41 (China Medical Bacteria Collection Management Center, strain number 44317).

[0032] 2. Cultivation and preparation of immunogen

[0033] Escherichia coli with serotypes K88, K99, and 987P were r...

Embodiment 2

[0074] Embodiment 2: the preparation of feed protein powder

[0075] Escherichia coli with serotype K88 was cultured according to the above-mentioned method, and cultured with shaking in an incubator at 35° C. to 40° C. for 16 hours. After the cultivation, centrifuge at 2500 rpm at 4°C for 30 minutes, discard the supernatant, and dilute the precipitate with phosphate buffer (pH 7.6) to adjust the viable bacteria content to 1.5×10 9 CFU / ml, then add 0.6% formaldehyde accounting for the total volume of the sterilizing solution, inactivate at 35°C for 16 hours, and use it as the immunogen to be used.

[0076] The immunogen was first immunized with the immunogen obtained by subcutaneous injection of healthy laying hens, and the immunization dose was 1.0ml. The first booster immunization was carried out 7 days after the primary immunization. A second booster immunization was performed 7 days after the first booster immunization. The obtained eggs were collected 7 days after the ...

Embodiment 3

[0079] Embodiment 3: Preparation of high-free protein powder for feeding

[0080] Escherichia coli with serotype K99 was cultured according to the above-mentioned method, and cultured with shaking in an incubator at 35° C. to 40° C. for 24 hours. After the cultivation, centrifuge at 3500 rpm at 4°C for 25 minutes, discard the supernatant, and dilute the precipitate with phosphate buffer (pH 7.6) to adjust the viable bacteria content to 3.0×10 6 CFU / ml, then add 0.7% formaldehyde to the total volume of the bacteria solution to be inactivated, inactivate at 36°C for 18 hours, and use it as the immunogen to be used.

[0081] The immunogen was initially immunized with the immunogen obtained by intramuscular injection of the abdomen of healthy laying hens, and the immunization dose was 3.0ml. The first booster immunization was carried out 10 days after the primary immunization. The second booster immunization was carried out 10 days after the first booster immunization. The obta...

technical field

[0001] The invention relates to an animal feed raw material, in particular to a feed protein powder containing specific Escherichia coli yolk antibody. The invention also relates to a preparation method and application of the protein powder. Background technique

[0002] Early weaning is an effective means to improve the efficiency of pig farms. However, in actual production, early weaning often leads to "early weaning syndrome" characterized by slow growth after weaning and diarrhea, which seriously affects the performance of piglets. How to recognize and solve weaning stress and help piglets transition to solid feed as soon as possible has always been the focus of attention of pig producers and nutritionists. The most common weaning stress disease in piglets is piglet diarrhoea. Studies have shown that the dietary factor is that allergy to dietary antigens in the intestine leads to damaging changes in the histology and morphological structure of the diges...

Examples

Embodiment 1

[0028] Embodiment 1: the preparation of feed protein powder

[0029] 1. Screening of strains

[0030] Escherichia coli (Escherichia coli) used in the present invention belongs to a kind of in Escherichia Enterobacteriaceae. Anaerobic, glucose-fermenting, lactose-decomposing bacteria. The size of Escherichia coli is 1.1-1.5 μm × 2.0-6.0 μm, with blunt round ends.

[0031] The present invention selects four kinds of Escherichia coli serotypes that are easy to cause diarrhea in weaned piglets after weaning by screening E. coli, namely K88 (China Medical Bacteria Preservation Management Center, strain number is 44742), K99 (China Medical Bacteria Preservation Management Center) Center, strain number 44820), 987P (China Institute of Veterinary Drug Control, strain number 83916) and F41 (China Medical Bacteria Collection Management Center, strain number 44317).

[0032] 2. Cultivation and preparation of immunogen

[0033] Escherichia coli with serotypes K88, K99, and 987P were r...

Embodiment 2

[0074] Embodiment 2: the preparation of feed protein powder

[0075] Escherichia coli with serotype K88 was cultured according to the above-mentioned method, and cultured with shaking in an incubator at 35° C. to 40° C. for 16 hours. After the cultivation, centrifuge at 2500 rpm at 4°C for 30 minutes, discard the supernatant, and dilute the precipitate with phosphate buffer (pH 7.6) to adjust the viable bacteria content to 1.5×10 9 CFU / ml, then add 0.6% formaldehyde accounting for the total volume of the sterilizing solution, inactivate at 35°C for 16 hours, and use it as the immunogen to be used.

[0076] The immunogen was first immunized with the immunogen obtained by subcutaneous injection of healthy laying hens, and the immunization dose was 1.0ml. The first booster immunization was carried out 7 days after the primary immunization. A second booster immunization was performed 7 days after the first booster immunization. The obtained eggs were collected 7 days after the ...

Embodiment 3

[0079] Embodiment 3: Preparation of high-free protein powder for feeding

[0080] Escherichia coli with serotype K99 was cultured according to the above-mentioned method, and cultured with shaking in an incubator at 35° C. to 40° C. for 24 hours. After the cultivation, centrifuge at 3500 rpm at 4°C for 25 minutes, discard the supernatant, and dilute the precipitate with phosphate buffer (pH 7.6) to adjust the viable bacteria content to 3.0×10 6 CFU / ml, then add 0.7% formaldehyde to the total volume of the bacteria solution to be inactivated, inactivate at 36°C for 18 hours, and use it as the immunogen to be used.

[0081] The immunogen was initially immunized with the immunogen obtained by intramuscular injection of the abdomen of healthy laying hens, and the immunization dose was 3.0ml. The first booster immunization was carried out 10 days after the primary immunization. The second booster immunization was carried out 10 days after the first booster immunization. The obta...