Polysaccharide purification system

Abstract

The fermentation liquor of the polysaccharide contains a plurality of components and covers a large range of molecular weight differences, so that the difficulty of extracting and purifying the polysaccharide is increased. In order to solve the problem, the polysaccharide purification system provided by the utility model comprises a homogenizing tank, an extraction tank, a precipitation tank and a filtering device, the homogenizing device is used for homogenizing fermentation liquor and uniformly mixing the fermentation liquor with alcohol; the extraction tank is used for extracting, decolorizing and deodorizing polysaccharides; the precipitation tank is used for precipitation and layering; and the filtering device is used for filtering and purifying the polysaccharide, so that the quality and the purity of the extracted polysaccharide are improved, and the production and multiple purposes of subsequent products are facilitated.

Description

Technical Field

[0001] The present application relates to a polysaccharide purification system, which purifies the polysaccharides produced by fermentation broth through multi-stage treatment. Background Art

[0002] Polysaccharides, as effective immunity enhancers and potential anti-cancer substances, have always been a hot commodity in the healthcare industry. In order to produce related products, the common method is to ferment animals, plants or fungi, and extract and purify the polysaccharide components from their fermentation broth. However, the fermentation broth of polysaccharides contains multiple components and covers a wide range of molecular weight differences, which increases the difficulty of extracting and purifying polysaccharides.

[0003] In order to solve the above problems, the present application provides a polysaccharide purification system, which improves the quality and purity of the extracted polysaccharides by multi-stage treatment of the polysaccharide fermentation broth, and is beneficial to the production of subsequent products and multiple uses. Utility Model Content

[0004] The present application provides a polysaccharide purification system, comprising a homogenizing tank for mixing fermentation liquid including mycelium for producing polysaccharides with alcohol to obtain a first mixture; an extraction tank for receiving the first mixture from the homogenizing tank and performing extraction, decolorization and deodorization treatment on the first mixture of the polysaccharides to obtain a second mixture containing the polysaccharides; a precipitation tank for receiving and standing the second mixture from the extraction tank, and the precipitation tank defines from top to bottom a first zone equipped with an upper discharge port, a second zone equipped with a middle discharge port, and a third zone equipped with a lower discharge port, respectively, to feed out the separated liquids from the first zone, the second zone and the third zone; and a filtering device for receiving and filtering the separated liquids from the first zone, the second zone and the third zone.

[0005] In a specific embodiment, a rotatable cutter is provided in the homogenizing tank, and the mycelium in the fermentation liquid is broken and mixed with the alcohol to obtain the first mixture under the conditions that the temperature of the homogenizing tank is not higher than 25°C and the rotation speed of the rotatable cutter is 15600 to 20800 rpm, wherein the concentration of the alcohol is 95 to 99 wt%, and the volume ratio of the fermentation liquid to the alcohol is 1:3.5 to 1:4.5.

[0006] In a specific embodiment, the extraction tank comprises a stirrer, and the first mixture is subjected to polysaccharide extraction, decolorization and deodorization treatment under the condition that the rotation speed of the stirrer is lower than the rotation speed of the rotatable cutter and the temperature is between 0 and 4° C. to obtain the second mixture. In a specific embodiment, the rotation speed of the stirrer is lower than 15600 rpm.

[0007] In one embodiment, the extraction tank extracts the first mixture for 3 to 10 hours.

[0008] In one embodiment, the second mixture is allowed to stand in the precipitation tank at a temperature not higher than 4° C. for 36 to 60 hours.

[0009] In a specific embodiment, the filtration device is provided with filter paper to filter the separation liquids from the first zone, the second zone and the third zone to purify the final polysaccharide product.

[0010] The polysaccharide purification system of the present application performs multi-stage treatment on the fermentation broth to improve the purity of the produced polysaccharides, thereby enhancing their quality and market availability. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 A schematic diagram showing the polysaccharide purification system of the present application.

[0012] Figure 2 A schematic diagram showing a polysaccharide purification system according to another embodiment of the present application.

[0013] Main component symbols

[0014] 10 Homogenizing tank

[0015] 101 Rotatable Tool

[0016] 20 Extraction tank

[0017] 20a First extraction tank

[0018] 20b Second extraction tank

[0019] 201 Mixer

[0020] 30,30a,30b,30c Sedimentation tank

[0021] 301 District 1

[0022] 3011 Upper discharge port

[0023] 302 District 2

[0024] 3022 middle discharge port

[0025] 303 District 3

[0026] 3033 Lower discharge port

[0027] 40,40a,40b,40c Filtering device

[0028] 401 filter paper

[0029] P1,P2,P3,P4,P5,P21,P22,P31,P32,P33,P41,P42,P43,P51,P52,P53 pipeline

[0030] V1, V2 valves. DETAILED DESCRIPTION

[0031] The following describes the implementation methods of the present application through specific specific examples, and those skilled in the art of the present application can easily understand the spirit, advantages and effects of the present application based on the contents described in this specification. However, the specific implementation methods of the present application are not intended to limit the present application, and the present application can also be implemented or applied through other different implementation methods, and the details contained in this specification can also be given different changes or modifications according to different viewpoints and applications without departing from the spirit of the present application.

[0032] When "including" or "comprising" components or steps described herein, unless otherwise specified, other components, components or other steps may be included, rather than excluding such other components, components or other steps. In addition, unless otherwise clearly specified herein, the singular forms "a", "an" and "the" described herein include plural referents, and "or" and "and / or" described herein are used interchangeably.

[0033] The numerical ranges described herein are inclusive and combinable. Any value falling within the numerical ranges described herein can be used as the maximum or minimum value to derive a sub-range; for example, the numerical range of "30% to 70%" should be understood to include any sub-range between the minimum value of 30% and the maximum value of 70%, such as: 30% to 60%, 40% to 70% and 45% to 65%. In addition, the percentage symbol "%" described herein, unless otherwise specified, means weight percentage.

[0034] In the present application, the fermentation broth for producing polysaccharides can be prepared from fungi, such as mushrooms. In a specific embodiment, the mushrooms are at least one selected from the group consisting of Agaricales, Aphyllophorales, Auriculariales, Boletales, Cantharellales, Ceratobasidiales, Dacrymycetales, Hymenochaetales, Lycoperdales, Phallales, Polyporales, Poriales, Russulales, Sclerodermatales, Hypocreales and Thelephorales; preferably, the mushrooms are at least one selected from the group consisting of Agaricales, Aphyllophorales, Polyporales, Russulales and Hypocreales. For example but not limited thereto, the mushrooms are at least one selected from the group consisting of Schizophyllum commue, Agaricus subrufescens, Armillaria mellea, Lentinula edodes, Phellinus linteus, Pleuritus citrinopileatus, Ganoderma lucidum, Trametes versicolor, Anthodia camphorate, Hericium erinaceus, Auricularia auricula, Flammulina velutipes, and Cordyceps militaris.

[0035] In a specific embodiment, the polysaccharide refers to the physiologically active ingredients and mushroom metabolites taken from the mycelium or fruiting body of mushrooms. Moreover, the polysaccharide described in the present application refers to β-glucan with a (1→3)-(1→6) bond structure mainly formed by the polymerization of glucose. This type of β-glucan has anti-inflammatory and antioxidant activities, and is a biological response modifying substance that can form a complex with biologically active regulatory proteins (such as immunomodulatory proteins and interferons) to activate the activity of macrophages in the body, enhance immunity, and reduce the chance of disease. In addition, the larger the molecular weight of the polysaccharide, the stronger the biopharmacological activity it exhibits.

[0036] In a non-limiting embodiment, the polysaccharide is a special active substance produced by the mycelium or fruiting body of mushrooms composed of at least one mushroom of different orders. In another specific embodiment, the polysaccharide can use a special active substance produced by the mycelium or fruiting body of a composite mushroom composed of at least two mushrooms of different orders, and the types of peptides, glycoproteins and amino acids are more diverse.

[0037] The polysaccharide purification system of the present application includes four stages of processing the fermentation broth, namely, a homogenization stage, an extraction stage, a precipitation stage, and a filtration stage. The above stages are respectively carried out in a homogenization tank, an extraction tank, a precipitation tank, and a filtration device. In addition, the polysaccharide purification system may further include processes such as drying, sterilizing, and packaging the purified polysaccharides, and these processes can be completed by known techniques or equipment in the art.

[0038] View this application Figure 1 , the fermentation liquid for producing polysaccharides is put into the homogenization tank 10 for homogenization. The homogenization tank 10 is provided with a rotatable tool 101, and the homogenization tank 10 is made to break the mycelium in the fermentation liquid at a temperature not higher than 25°C and a rotation speed of the rotatable tool 101 in a high speed range of 15600 to 20800rpm, and mixed with the alcohol to obtain the first mixture. In other embodiments, the rotation speed of the rotatable tool 101 is, for example, a high speed range of 15600 to 16900, 16900 to 18200, 18200 to 19500 or 19500 to 20800rpm. The rotation speed of the rotatable tool 101 can also be set to 15600, 16000, 17000, 18000, 19000, 20000 or 20800rpm. In addition, the temperature of the homogenizing tank 10 is usually controlled at a temperature not higher than 25° C., for example, about 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25° C. At this stage, alcohol is added to the homogenizing tank 10, and the fermentation liquid and the alcohol are uniformly mixed to obtain a first mixture, wherein the volume ratio of the fermentation liquid to the alcohol is 1:3.5 to 1:4.5. Moreover, the alcohol described herein is ethanol with a concentration of 95 to 99 wt%.

[0039] The extraction stage described in the present application is used to extract the polysaccharide in the first mixture, and can decolorize and deodorize the polysaccharide. In this stage, the extraction tank 20 receives the first mixture from the homogenization tank 10 through the pipeline P1, and the extraction tank 20 includes a stirrer 201, and the first mixture is stirred under the condition that the rotation speed of the stirrer 201 is lower than the rotation speed of the rotatable cutter 101 and the temperature is between 0 and 4°C, for example, about 0, 1, 2, 3 or 4°C, to extract the polysaccharide, and achieve the purpose of decolorization and eliminating the fermentation odor to obtain the second mixture. In a specific embodiment, the rotation speed of the stirrer is lower than 15600rpm. For example, it is 15000, 14000, 13000, 12000, 11000, 10000, 9000, 8000, 7000, 6000, 5000, 4000, 3000, 2000 or 1000rpm. On the other hand, the polysaccharide purification system of the present application is divided into a homogenization tank 10 and an extraction tank 20 to optimize the polysaccharide purification efficiency. Specifically, the purpose of the homogenization treatment is to quickly break the mycelium in the fermentation broth. Compared with the extraction treatment of 3 to 10 hours, the time required for the homogenization treatment is shorter. Therefore, the homogenization and extraction are performed in stages. The first mixture with broken mycelium can be fed to the extraction tank for extraction, and the homogenization tank can be used for the next batch of homogenization treatment. In addition, if Figure 2 As shown, the polysaccharide purification system of the present application can set up multiple extraction tanks in parallel, for example, including a first extraction tank 20a and a second extraction tank 20b. When the first extraction tank 20a is extracting, the second extraction tank 20b can wait for or simultaneously receive and extract the next batch of the first mixture from the homogenization tank 10.

[0040] During the precipitation stage, a precipitation tank 30 is configured to receive the second mixture from the extraction tank 20 through the pipeline P2. When the second mixture from the extraction tank 20 is allowed to stand, the temperature of the precipitation tank 30 is 0 to 4°C, for example, about 0, 1, 2, 3 or 4°C, and the second mixture is allowed to stand for 36 to 60 hours. The second mixture after standing is roughly divided into three layers: upper, middle and lower due to the molecular weight difference of the polysaccharide. Therefore, the precipitation tank 30 defines a first zone 301 with an upper discharge port 3011, a second zone 302 with a middle discharge port 3022 and a third zone 303 with a lower discharge port 3033 from top to bottom, so as to feed out the separated liquids of the first zone 301, the second zone 302 and the third zone 303. In addition, the volume ratio of the first zone 301, the second zone 302 and the third zone 303 of the sedimentation tank 30 is 1:1:1 to 1:2:1, and the upper discharge port 3011 is located at the lower edge of the first zone 301 of the sedimentation tank 30, the middle discharge port 3022 is located at the lower edge of the second zone 302 of the sedimentation tank 30, and the lower discharge port 3033 is located at the lower edge of the third zone 303 of the sedimentation tank 30. The separated liquid collected in the first zone 301 can be used for skin care products such as lotion; the separated liquid collected in the second zone 302 can be used for skin care products in the form of gel or emulsion for repairing skin and moisturizing; and the separated liquid collected in the third zone 303 can be used for anti-inflammatory skin care products or external medicines.

[0041] In the filtration stage, the separated liquids are fed into the filtration device 40 for filtration. A single filtration device 40 can be used to simultaneously receive and filter the separated liquids from the first zone 301, the second zone 302 and the third zone 303 through, for example, pipelines P3, P4, and P5. The filtration device 40 is provided with a filter paper 401 with a mesh size of 100 to 300 to filter the separated liquids from the first zone 301, the second zone 302 and the third zone 303 to purify the final polysaccharide product.

[0042] In another embodiment of the polysaccharide purification system of the present application, Figure 2As shown, the polysaccharide purification system of the present application can be provided with multiple extraction tanks in parallel, for example, including a first extraction tank 20a and a second extraction tank 20b. When the first extraction tank 20a is extracting, the second extraction tank 20b can wait for or simultaneously receive and extract the next batch of the first mixture from the homogenization tank 10. In addition, this embodiment also includes multiple precipitation tanks 30a, 30b, 30c, and the second mixture from the first extraction tank 20a and / or the second extraction tank 20b fed into the precipitation tanks 30a, 30b, 30c is received and controlled through pipelines P21, P22 and valves V1, V2. Moreover, the sedimentation tanks 30a, 30b, 30c are further connected to the filtering devices 40a, 40b, 40c through the fluids of pipelines P31, P32, P33, P41, P42, P43, P51, P52, P53 to filter the separated liquid from the first zone of each sedimentation tank 30a, 30b, 30c through the pipelines P31, P32, P33, filter the separated liquid from the second zone of each sedimentation tank 30a, 30b, 30c through the pipelines P41, P42, P43, and filter the separated liquid from the third zone of each sedimentation tank 30a, 30b, 30c through the pipelines P51, P52, P53.

[0043] The features and effects of the present application are further illustrated below through specific examples, but are not intended to limit the scope of the present application.

[0044] Example 1

[0045] The polysaccharide purification system of the present application comprises a four-stage treatment of the fermentation broth, which comprises the following steps:

[0046] Step 1: Reference Figure 1 As shown, the fermentation liquid and 95wt% alcohol of 4 times the volume of the fermentation liquid are added to the homogenizing tank 10, and the rotation speed of the rotatable cutter 101 is set to 20800rpm, and the homogenizing tank 10 is kept at 25°C to break the mycelium in the fermentation liquid, and the first mixture is obtained after mixing the fermentation liquid and the alcohol for 2 hours. The fermentation liquid used in this embodiment adopts Ganoderma lucidum as the strain, and is obtained through the steps of activation, acclimation, mycelium increment and final fermentation.

[0047] Step 2: Reference Figure 1 As shown, the first mixture prepared in step 1 is fed into an extraction tank 20, and the rotation speed of the agitator 201 of the extraction tank 20 is set to 1000 rpm, while the extraction tank 20 is maintained at 0°C to extract the polysaccharides of the first mixture, and decolorize and deodorize the first mixture. After 6 hours, a second mixture containing polysaccharides is obtained.

[0048] Step 3: Reference Figure 1As shown, the second mixture collected in step 2 is fed into the precipitation tank 30, and the temperature of the precipitation tank 30 is set to 0°C, and the second mixture is left to stand for 48 hours. The volume ratio of the first zone 301, the second zone 302 and the third zone 303 of the precipitation tank 30 is 1:2:1, and the separation liquid is fed from the first zone 301, the second zone 302 and the third zone 303 in sequence to the filtering device 40 for the next stage of filtering treatment.

[0049] Step 4: Read Figure 1 As shown, the separated liquid obtained in step 3 is respectively put into a filtering device 40 provided with a 200-mesh filter paper for filtration to obtain three batches of polysaccharides with different molecular weights. The above embodiments are only illustrative and are not intended to limit the present application. Those skilled in the art may modify and change the above embodiments without violating the spirit and scope of the present application. Therefore, the scope of protection of the present application is defined by the attached claims, and as long as it does not affect the effect and implementation purpose of the present application, it should be covered in this disclosed technical content.

Claims

1. A polysaccharide purification system, comprising: A homogenizing tank for mixing a fermentation liquid containing mycelium for producing polysaccharides with alcohol to obtain a first mixture; An extraction tank, used for receiving the first mixture from the homogenization tank, and performing extraction, decolorization and deodorization treatment on the first mixture to obtain a second mixture containing the polysaccharide; a settling tank for receiving and standing the second mixture from the extraction tank, wherein the settling tank defines, from top to bottom, a first zone with an upper discharge port, a second zone with a middle discharge port, and a third zone with a lower discharge port, respectively, to feed out the separated liquids from the first zone, the second zone, and the third zone; and The filtering device is used to receive and filter the separated liquid from the first zone, the second zone and the third zone.

2. The system according to claim 1, wherein: The homogenizing tank is provided with a rotatable cutter for breaking the mycelium in the fermentation liquid and mixing it with the alcohol to obtain the first mixture.

3. The system according to claim 2, wherein: The extraction tank comprises a stirrer for performing polysaccharide extraction, decolorization and deodorization treatment on the first mixture to obtain the second mixture.

4. The system according to claim 1, wherein: The volume ratio of the first zone, the second zone and the third zone of the precipitation tank is 1:1:1 to 1:2:

1.

5. The system according to claim 1, wherein: The upper discharge port is located at the lower edge of the first zone of the sedimentation tank, the middle discharge port is located at the lower edge of the second zone of the sedimentation tank, and the lower discharge port is located at the lower edge of the third zone of the sedimentation tank.

6. The system according to claim 1, wherein: The filtering device is provided with filter paper to filter the separation liquid from the first zone, the second zone and the third zone to purify the final polysaccharide product.

7. The system according to claim 6, wherein: The mesh size of the filter paper is 100 to 300 meshes.

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