Tumor organ passage tool

Abstract

The utility model discloses a tool for passage of tumorous organs, which comprises a first handle, a second handle, a first connecting rod, a second connecting rod, a cutter assembly and a container, the first handle is connected with the first connecting rod, the second handle is connected with the second connecting rod, the first handle is hinged with the second handle, and the cutter assembly is connected with the container. The cutter assembly is arranged at one end of the first connecting rod, the container is arranged at one end of the second connecting rod, the container is used for fixing the similar organs, and under the condition that the first handle and the second handle move relatively, the cutter assembly can move in the container and abut against the inner bottom of the container. Therefore, the segmentation of the organoid is realized. The device is simple to operate, the passage efficiency is improved, the uniformity of passage samples is ensured, the mechanical damage can be reduced, and the survival rate is increased.

Description

Technical Field

[0001] The utility model relates to the technical field of medical tools, in particular to a tool for tumor organoid passage. Background Art

[0002] Currently, tumor organoids are mainly cultured by microdissection under a microscope with tissue scissors and tweezers. Usually, the spherical organoids with a diameter of about 1.00 mm floating in the culture medium need to be evenly cut and separated into 4 parts of the same size. The current method of relying on ordinary tissue scissors and tweezers to cut and separate organoids requires high operational proficiency on the one hand, and on the other hand, it is difficult to fix the organoids with ordinary tissue scissors and tweezers, and the consistency of cutting is difficult to ensure. Therefore, it is necessary to design a special tool to improve the efficiency of culture and reduce mechanical damage during the culture process. Utility Model Content

[0003] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a tool for tumor organoid culture to improve the culture efficiency.

[0004] The technical solution of the utility model is:

[0005] A tool for tumor organoid culture, comprising a first handle, a second handle, a first connecting rod, a second connecting rod, a tool assembly and a container, wherein the first handle is connected to the first connecting rod, the second handle is connected to the second connecting rod, the first handle and the second handle are hinged, the tool assembly is arranged at one end of the first connecting rod, the container is arranged at one end of the second connecting rod, and the container is used to fix the organoid, and when the first handle and the second handle are relatively movable, the tool assembly can move in the container and abut against the bottom of the container to achieve the segmentation of the organoid.

[0006] Further, the tool assembly includes at least one blade.

[0007] Furthermore, the tool assembly includes a plurality of blades, and the plurality of blades are distributedly connected in a concentric array.

[0008] Furthermore, the tool assembly includes two blades, and the two blades are vertically connected to form a cross-shaped tool assembly.

[0009] Furthermore, the length of the blade is 0.92-0.97 mm.

[0010] Furthermore, the container is hemispherical.

[0011] Furthermore, the inner diameter of the container is 0.98-1.02 mm.

[0012] Furthermore, the length of the first handle is equal to the length of the second handle, the length of the first connecting rod is equal to the length of the second connecting rod, and the total length of the first handle and the first connecting rod is 8 to 12 cm.

[0013] Furthermore, a limit rod is provided on the first handle or the second handle.

[0014] Furthermore, it also includes a hinge assembly, which includes a first hinge plate, a second hinge plate and a fixed shaft, the first hinge plate is provided with a first connecting block and a second connecting block, the first connecting block and the second connecting block are respectively connected to the first handle and the first connecting rod, the second hinge plate is provided with a third connecting block and a fourth connecting block, the third connecting block and the fourth connecting block are respectively connected to the second handle and the second connecting rod, the first hinge plate and the second hinge plate are respectively provided with a first through hole and a second through hole, the first hinge plate and the second hinge plate are rotatably mounted on the fixed shaft through the first through hole and the second through hole.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] The utility model arranges a cutter assembly and a container on two connecting rods respectively. The container is used to fix the organoid. The cutter assembly, in cooperation with the first handle and the second handle, cuts the organoid fixed in the container. The operation is simple, the efficiency of the culture is improved, and the uniformity of the cultured samples can be ensured while reducing mechanical damage and increasing the survival rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0018] Figure 1 It is a partial stereoscopic view of a tool for tumor organoid culture according to an embodiment of the utility model.

[0019] Figure 2 The present invention is a plan view of a tool for tumor organoid culture according to an embodiment of the present invention.

[0020] Figure 3 for Figure 1 A magnified schematic diagram of center A.

[0021] Figure 4This is a structural diagram of a hinge assembly in an upper mold assembly in a tool for tumor organoid culture according to an embodiment of the utility model, wherein: (a) a first hinge piece; (b) a second hinge piece; (c) a rotating shaft; and (4) a hinge assembly.

[0022] Figure 5 It is a stereoscopic view of the rotating shaft in the lower mold assembly of a tool for tumor organoid culture according to an embodiment of the utility model.

[0023] In the figure, 1 is the first handle, 2 is the second handle, 3 is the first connecting rod, 4 is the second connecting rod, 5 is the tool assembly, 501 is the blade, 6 is the container, 7 is the hinge assembly, 701 is the first hinge plate, 702 is the second hinge plate, 703 is the fixed axis, 7031 is the limit block, 704 is the first connecting block, 705 is the second connecting block, 706 is the third connecting block, 707 is the fourth connecting block, 708 is the first through hole, 709 is the second through hole, and 8 is the limit rod. DETAILED DESCRIPTION

[0024] The following describes the implementation of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific implementations, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0025] In the description of the present invention, unless otherwise specified, "multiple" means two or more; the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third" and the like are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0026] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0027] The utility model will now be further described in conjunction with the accompanying drawings.

[0028] The present invention provides a tool for passage of tumor organoids, such as Figure 1 and Figure 2 As shown, the tool for tumor organoid culture includes a first handle 1, a second handle 2, a first connecting rod 3, a second connecting rod 4, a tool assembly 5 and a container 6. The first handle 1 is connected to the first connecting rod 3, the second handle 2 is connected to the second connecting rod 4, the first handle 1 and the second handle 2 are hinged, the tool assembly 5 is arranged at one end of the first connecting rod 3, the container 6 is arranged at one end of the second connecting rod 4, and the container 6 is used to fix the organoid. When the first handle 1 and the second handle 2 are relatively movable, the tool assembly 5 can move in the container 6 and abut against the bottom of the container 6 to achieve the segmentation of the organoid.

[0029] When the tool for tumor organoid passage is in operation, the first handle 1 and the second handle 2 are hinged, for example, the structure can be similar to scissors, that is, the first handle 1 and the second handle 2 are hinged at a fixed point through a hinge assembly 7. At this time, the operator holds the first handle 1 and the second handle 2, and the first connecting rod 3 and the second connecting rod 3 connected thereto can be made to move relative to each other by controlling the first handle 1 and the second handle 2. First, by manipulating the first handle 1 and the second handle 2, the tool assembly 5 is located outside the container 6 and has a certain distance from the container 6. The distance should at least ensure that the tumor organoid can be placed in the container 6. The container 6 plays the role of fixing the tumor organoid. Therefore, the specific shape and size of the container 6 should be determined according to the size of the tumor organoid. After the tumor organoid is placed in the container 6, the first handle 1 and the second handle 2 are continued to be manipulated so that the tool assembly 5 enters the container 6 to cut the tumor organoid, thereby realizing the passage of the tumor organoid.

[0030] Compared with the current method of cutting and separating organoids using tissue scissors and tweezers, the tool for tumor organoid passaging proposed in this application significantly improves the passaging efficiency of tumor organoids and further reduces the requirement for operating proficiency. It can reduce mechanical damage (for example, compared with the existing method, it can at least reduce the damage of tweezers) while ensuring the uniformity of the passaging samples, thereby increasing the survival rate.

[0031] In some embodiments, the cutter assembly 5 includes at least one blade. The number of blades is determined according to the number of pieces into which the tumor organoid needs to be cut. Assuming that the tumor organoid needs to be cut into n pieces, the number of blades is set to 0.5n, where n is a natural number greater than 0.

[0032] When the number of blades is greater than or equal to 2, the multiple blades are distributedly connected in a concentric array.

[0033] For example, in a scenario where an organoid with a diameter of about 1.00 mm needs to be evenly cut and separated into four portions of the same size, Figure 1 and Figure 3 As shown, the tool assembly 5 includes two blades 501, and the two blades 501 are vertically connected to form a cross-shaped tool assembly 5. In the tool assembly 5 with two blades 501, when the tool assembly 5 is located in the container, its two ends should be as close to the inner wall of the container 6 as possible, for example, the distance should not exceed 0.1 mm.

[0034] When the present application is applied to the scenario as shown in the above example, the present embodiment can use a hemispherical shape with a diameter of 1.00 mm as a container, design the cutting blade into two perpendicularly intersecting semicircles with a diameter of 0.95 mm, the lengths of the first handle 1 and the second handle 2 are equal, the lengths of the first connecting rod 1 and the second connecting rod 2 are equal, and the length of the first connecting rod 3 plus the first handle 1 is 10.00 cm. When performing passaging, the organoid is fixed with the container 5, the first handle 1 and the second handle 2 are manipulated, and the blade 501 cuts the organoid in the container 5, so that the organoid can be evenly divided into 4 parts.

[0035] In some embodiments, the tumor organoid passage tool further comprises an articulated component 7, such as Figure 4 As shown, it is a structural diagram of the hinge assembly 7, wherein Figure 4 (a) shows a structural diagram of the first hinged piece, Figure 4 (b) shows the structure of the second hinged piece. Figure 4 (c) shows the structure diagram of the fixed axis. Figure 4 (d) shows the overall structure of the hinge assembly 7, combined with Figure 4 As shown in (a)-(d), the hinge assembly 7 includes a first hinge plate 701, a second hinge plate 702 and a fixed shaft 703, the first hinge plate 701 is provided with a first connecting block 704 and a second connecting block 705, the first connecting block 704 and the second connecting block 705 are respectively connected to the first handle 1 and the first connecting rod 3, the second hinge plate 702 is provided with a third connecting block 706 and a fourth connecting block 707, the third connecting block 706 and the fourth connecting block 707 are respectively connected to the second handle 2 and the second connecting rod 4, the first hinge plate 701 and the second hinge plate 702 are respectively provided with a first through hole 708 and a second through hole 709, the first hinge plate 701 and the second hinge plate 702 are rotatably mounted on the fixed shaft 703 through the first through hole 708 and the second through hole 709.

[0036] Exemplarily, the first handle 1 and the first connecting rod 3 can be connected to the first connecting block 704 and the second connecting block 705 respectively in an integral manner. Similarly, the second handle 2 and the second connecting rod 4 can be connected to the third connecting block 706 and the fourth connecting block 707 respectively in an integral manner. In this way, the first handle 1 and the first connecting rod 3 can be stably mounted on the first hinge plate 701, and the second handle 2 and the second connecting rod 4 can be stably mounted on the second hinge plate 702. The first hinge plate 701 and the second hinge plate 702 can both be rotatably mounted on a fixed axis 703. In this way, the first handle 1 and the second handle 2 can be manipulated to rotate the first hinge plate 701 and the second hinge plate 702 on the fixed axis 703, so that the first connecting rod 3 and the second connecting rod 4 can move relative to each other, thereby realizing the cutting of tumor organoids.

[0037] In some embodiments, Figure 5 As shown, a stopper 7031 is respectively disposed at both ends of the fixed shaft 703, and the stopper 7031 plays a stopper role to prevent the first hinge piece 701 and the second hinge piece 702 from coming out. During assembly, a stopper 7031 can be integrally connected to one end of the fixed shaft 703, and after the end of the fixed shaft 703 not connected with the stopper 7031 passes through the first through hole 708 and the second through hole 709, a stopper 7031 is fixed to the end again by a method including but not limited to welding.

[0038] In some embodiments, Figure 2 As shown, a limit rod 8 is provided on the first handle or the second handle 2, and the limit rod 8 is used to ensure that when the limit rod 8 abuts against the first handle 1, the blade assembly 5 just contacts the bottom of the container 6. In this way, the movable stroke between the first handle 1 and the second handle 2 can be limited to prevent the blade assembly 5 from further applying pressure to the bottom of the container 6 and causing wear to the bottom of the container 6.

[0039] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the technical solutions described in the above embodiments can still be modified, or some or all of the technical features can be replaced by equivalents. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and specification of the present invention.

Claims

1. A tool for passage of tumor organoids, characterized in that: The invention comprises a first handle, a second handle, a first connecting rod, a second connecting rod, a tool assembly and a container, wherein the first handle is connected to the first connecting rod, the second handle is connected to the second connecting rod, the first handle and the second handle are hinged, the tool assembly is arranged at one end of the first connecting rod, the container is arranged at one end of the second connecting rod, and the container is used to fix the organoid, and when the first handle and the second handle are relatively movable, the tool assembly can move in the container and abut against the bottom of the container to achieve the segmentation of the organoid.

2. The tool for tumor organoid culture according to claim 1, characterized in that: The knife assembly includes at least one blade.

3. The tool for tumor organoid culture according to claim 1, characterized in that: The tool assembly includes a plurality of blades which are distributed and connected in a concentric array.

4. The tool for tumor organoid culture according to claim 1, characterized in that: The tool assembly comprises two blades, which are vertically connected to form a cross-shaped tool assembly.

5. The tool for tumor organoid passaging according to any one of claims 2 to 4, characterized in that: The length of the blade is 0.92-0.97 mm.

6. The tool for tumor organoid culture according to claim 1, characterized in that: The container is hemispherical.

7. The tool for tumor organoid culture according to claim 6, characterized in that: The inner diameter of the container is 0.98-1.02 mm.

8. The tool for tumor organoid culture according to claim 1, characterized in that: The length of the first handle is equal to the length of the second handle, the length of the first connecting rod is equal to the length of the second connecting rod, and the total length of the first handle and the first connecting rod is 8 to 12 cm.

9. The tool for tumor organoid culture according to claim 8, characterized in that: A limit rod is provided on the first handle or the second handle.

10. The tool for tumor organoid passaging according to claim 1, characterized in that: It also includes a hinge assembly, which includes a first hinge plate, a second hinge plate and a fixed shaft, the first hinge plate is provided with a first connecting block and a second connecting block, the first connecting block and the second connecting block are respectively connected to the first handle and the first connecting rod, the second hinge plate is provided with a third connecting block and a fourth connecting block, the third connecting block and the fourth connecting block are respectively connected to the second handle and the second connecting rod, the first hinge plate and the second hinge plate are respectively provided with a first through hole and a second through hole, the first hinge plate and the second hinge plate are rotatably mounted on the fixed shaft through the first through hole and the second through hole.

Images