Image acquisition system for microscopic object

Abstract

The utility model belongs to the field of image acquisition, and particularly relates to an image acquisition system of a microscopic object. The system comprises an image acquisition device; the image acquisition device comprises a shooting device and an imaging device which is connected with a lens of the shooting device and is used for amplifying and imaging; the moving device is used for mounting the image acquisition device and moving the image acquisition device back and forth along the focal length change direction of the lens; through the magnification imaging device connected with the lens of the shooting device and the moving device capable of moving the image acquisition device back and forth along the focal length change direction of the lens of the shooting device, the shooting device has the magnification function of a microscope so as to form a photomicrography system suitable for a microscopic object; according to the technical scheme, the focal length of the lens of the shooting device can be adjusted through the moving device so as to achieve focusing of different areas of the microscopic object, the focusing of the different areas can be achieved without moving the object carrying platform, and therefore operation on the moving object carrying platform can be simplified when the microscopic object image is collected.

Description

Technical Field

[0001] The utility model belongs to the field of image acquisition, and in particular relates to an image acquisition system for microscopic objects. Background Art

[0002] The three-dimensional model of microscopic objects is a means of using computer technology to truly restore the external morphology and internal structure of microscopic objects. It can provide more intuitive and comprehensive morphological information of microscopic objects, which is convenient for researchers to classify, identify and compare and analyze. In addition, the three-dimensional model of microscopic objects can be used as an effective teaching and popular science tool to enhance the popularity and visualization of the corresponding microscopic object research, improve the public's cognitive level, and also provide effective help for the training and learning of students and professionals in related colleges and universities.

[0003] The Chinese utility model patent application with the publication number CN112258390A discloses a method for generating high-precision microscopic virtual learning resources, in which a super-depth three-dimensional microscope system is used. According to the overlapping shooting requirements, the specimen surface is photographed at a certain frequency from three directions: vertical, inclined, and adjacent, by moving the loading platform, focusing on different areas of the specimen, and sequentially completing the information collection of the microscopic details of the specimen object, and obtaining a high-definition series of images of the specimen for generating a 3D model of the specimen surface. However, the image acquisition system of the microscopic object composed of the super-depth three-dimensional microscope system and the loading platform used in this method needs to change the shooting angle and focus on different areas through the moving loading platform when performing image acquisition, which will result in the operation of the moving loading platform being more complicated when performing image acquisition of microscopic objects at different focus positions. Utility Model Content

[0004] The utility model aims to provide an image acquisition system for microscopic objects, which is used to solve the problem of complicated operation of a mobile loading platform when acquiring images of microscopic objects at different focus positions in the prior art.

[0005] In order to achieve the above-mentioned purpose, the utility model provides an image acquisition system for microscopic objects, including an image acquisition device; the image acquisition device includes a shooting device and an imaging device for connecting to the lens of the shooting device and performing magnified imaging; and also includes a moving device for installing and moving the image acquisition device back and forth along the focal length change direction of the lens.

[0006] Furthermore, the moving device is a slide rail sliding assembly, which includes a basic part and a sliding part. The basic part is used to be placed still in a certain position, and the sliding part is used to install the image acquisition device and is slidably installed relative to the basic part to move the image acquisition device by sliding back and forth along the focal length change direction of the lens.

[0007] Furthermore, it also includes a stepping motor, which is used to connect with the moving device to drive the moving device to move in steps, thereby driving the image acquisition device to move in steps.

[0008] Furthermore, it also includes a stage; the stage includes a rotating platform; the rotating platform is used to place the image acquisition object and drive the image acquisition object to rotate axially, so as to expose different angles of the image acquisition object within the field of view of the image acquisition device.

[0009] Furthermore, the stage also includes an XY axis adjustment seat, the rotating platform is installed on the adjustment seat, and the adjustment seat is used to drive the rotating platform to change its position relative to the image acquisition device in space, so as to adjust the position of the image acquisition object to within a set field of view of the image acquisition device; the plane formed by the XY axes is perpendicular to the axis direction of the axial rotation.

[0010] Furthermore, the imaging device is a microscope objective lens; the image acquisition system also includes a lens adapter ring for connecting the lens of the shooting device with the microscope objective lens.

[0011] Furthermore, the microscope objective lens is an infinite microscope objective lens with a certain magnification ratio.

[0012] Furthermore, the lens of the photographing device is a telephoto lens.

[0013] Furthermore, the stage also includes a mounting component for placing the image acquisition object and for fixing the image acquisition object relative to the rotating platform.

[0014] Furthermore, the mounting component is a specimen needle; a recessed portion is provided at the center position corresponding to the axial rotation of the rotating platform, the mounting component is arranged in the recessed portion, perpendicular to the plane of the rotating platform, and the depth of the recessed portion is less than the height of the mounting component.

[0015] The above-mentioned technical scheme of the utility model improves the image acquisition device in the image acquisition system of microscopic objects on the basis of the existing image acquisition system of microscopic objects, and its beneficial effects include: through an imaging device connected to the lens of the shooting device and performing magnified imaging and a moving device that can move the image acquisition device back and forth along the focal length change direction of the lens of the shooting device, the shooting device has a magnification function similar to that of a microscope to form a microscopic photography system suitable for microscopic objects, and at the same time, the lens focal length of the shooting device can be adjusted by the moving device to achieve focusing on different areas of the image acquisition object (i.e., the microscopic object), that is, the focusing on different areas does not need to be achieved by moving the object carrier, thereby simplifying the operation of the moving carrier when capturing images of microscopic objects at different angles and different focus areas. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural schematic diagram of an image acquisition device in the image acquisition system for microscopic objects in an embodiment of the image acquisition system for microscopic objects of the utility model;

[0017] Figure 2 It is a structural schematic diagram of a stage in the image acquisition system for microscopic objects in the embodiment of the image acquisition system for microscopic objects of the utility model;

[0018] Figure 3 It is a schematic diagram of the positional relationship between the image acquisition device and the stage in the image acquisition system for microscopic objects in the embodiment of the utility model;

[0019] In the figure, 1 is a slide rail base, 2 is a slide rail track, 3 is a single-lens reflex viewfinder camera body, 4 is a camera lens fixing ring, 5 is an infinity lens SLR camera lens, 6 is a lens adapter ring, 7 is a four-fold magnification infinity flat-field microscope objective, 8 is a slide rail bracket, 9 is an X-axis adjustment knob, 10 is a specimen needle, 11 is an R-axis rotating platform, 12 is an XY-axis adjustment seat, 13 is a Y-axis adjustment knob, and 14 is an R-axis adjustment knob. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments.

[0021] Embodiment of the image acquisition system of microscopic objects

[0022] This embodiment provides a technical solution of a microscopic object image acquisition system, including an image acquisition device; Figure 1 The image acquisition device includes a shooting device and an imaging device for connecting to the lens of the shooting device and performing magnification imaging; and also includes a moving device for installing and moving the image acquisition device forward and backward along the focal length change direction of the lens of the shooting device. In this embodiment, the shooting device is a single-lens reflex camera with a continuous shooting function. In other embodiments, other devices capable of shooting images can also be used. Therefore, by connecting to the lens of the shooting device and performing magnification imaging with the imaging device and the moving device capable of moving the image acquisition device forward and backward along the focal length change direction of the lens of the shooting device, the shooting device has a magnification function similar to that of a microscope, thereby forming a microscopic photography system suitable for microscopic objects, and the lens focal length of the shooting device can be adjusted by the moving device to achieve focusing on different areas of the image acquisition object (i.e., the microscopic object), that is, focusing on different areas does not need to be achieved by moving the object platform, thereby simplifying the operation of the moving platform when capturing images of microscopic objects at different angles and different focus areas.

[0023] In this embodiment, Figure 1 As shown, the moving device is a slide rail sliding assembly, which includes a basic component and a sliding component; the basic component is used to be stationary at a certain position to stabilize the entire slide rail sliding assembly, such as Figure 1 The slide rail base 1 and the slide rail track 2 are both part of the basic component; the sliding component is used to install the image acquisition device and is slidably installed relative to the basic component to slide back and forth along the focal length change direction of the lens of the shooting device to move the image acquisition device, such as Figure 1 The slide rail bracket 8 slidably installed on the slide rail 2 is a sliding part; thus, it can not only meet the demand of moving the image acquisition device forward and backward along the focal length change direction of the lens of the shooting device, but also ensure that the movement of the image acquisition device is limited to a certain range, which is convenient for accurate control of the moving position of the image acquisition device; since the structure that enables the sliding part to be slidably installed relative to the basic part to achieve the effect of sliding movement along a specific direction belongs to the prior art, it will not be repeated here. In other embodiments, the moving device can also be a rolling wheel installed under the image acquisition device, a moving track for conveying the image acquisition device, a pulling device and a guide slot for cooperating to realize the movement of the image acquisition device, etc., as long as the effect of sliding the image acquisition device forward and backward along the focal length change direction of the lens of the shooting device can be achieved.

[0024] Specifically, the image acquisition system of microscopic objects in this embodiment also includes a stepper motor, which is used to connect with the mobile device to drive the mobile device to move step by step, thereby driving the image acquisition device to move step by step. In this way, the moving distance and moving frequency of the image acquisition device can be automatically controlled, and the focus depth of the camera can be automatically and evenly controlled by taking pictures during each stepping movement, so that RGB images of different focus areas can be obtained more efficiently; specifically, the stepper motor in this embodiment is directly installed in an integrated manner with the slide rail base 1, so Figure 1 It is not shown in the figure; the rotation angle of the stepper motor is proportional to the input pulse signal, so the rotation angle and speed of the motor can be accurately controlled by controlling the number and frequency of the pulse signal. The output shaft of the stepper motor is connected to the sliding part. Usually, the rotation of the motor is converted into the linear motion of the sliding part through transmission devices such as gears and screws. In other embodiments, the stepper motor body can also be designed separately from the moving device, as long as the connection relationship between the two can drive the moving device to move step by step; and, since the structure of controlling the stepping sliding of the sliding part by a stepper motor belongs to the prior art, it will not be repeated here.

[0025] The image acquisition system of the microscopic object also includes a stage; Figure 2 , the stage includes a rotating platform, that is Figure 2The R-axis rotating platform 11 in the image acquisition device; the rotating platform is used to place the image acquisition object and drive the image acquisition object to rotate axially, so as to expose different angles of the image acquisition object to the set field of view of the image acquisition device; thereby, it is convenient to acquire images of different angles of the image acquisition object by rotating the rotating platform. In addition, in this embodiment, the stage also includes an XY-axis adjustment seat 12, on which the rotating platform is installed, and the adjustment seat is used to drive the rotating platform to change its position relative to the image acquisition device in space; wherein, the plane formed by the XY axis is perpendicular to the axis direction of the axial rotation of the rotating platform, that is, perpendicular to the R axis. Thus, the position of the image acquisition object can be adjusted to a more reasonable field of view of the image acquisition device by adjusting the rotating platform in the XY-axis direction, without moving the entire stage or the entire mobile device, thereby simplifying the operation process of enabling the lens in the image acquisition device to align with the image acquisition object placed on the rotating platform. Specifically, in this embodiment, the R-axis rotating platform 11 is moved along the XY-axis direction through the XY-axis adjustment seat 12, and the R-axis rotating platform 11 itself can rotate along the R-axis; specifically, the total height of the R-axis rotating platform 11 and the XY-axis adjustment seat 12 is 55mm, the translation accuracy of the X-axis and Y-axis of the XY-axis adjustment seat 12 is 0.1mm, and the rotation accuracy of the R-axis of the R-axis rotating platform 11 is 1°. Specifically, the X-axis adjustment knob 9, the Y-axis adjustment knob 13, and the R-axis adjustment knob 14 are used to realize the corresponding adjustment of the X-axis, Y-axis of the XY-axis adjustment seat 12 and the R-axis direction of the R-axis rotating platform 11; by adjusting this The three knobs can fine-tune the movement or rotation distance or angle of the three axes to achieve a more precise positioning effect; since the structures of the X-axis adjustment knob, the Y-axis adjustment knob and the R-axis adjustment knob for adjusting the position in the corresponding directions all belong to the application of the prior art, they will not be repeated here; in other embodiments, rotating platforms and adjustment seats of other specifications can also be used, and the adjustment seat is not necessarily in the form of a base installed on a horizontal plane, but can also be fixed on a vertical wall or a support frame; the rotating platform is not necessarily circular; since the structure that can achieve position adjustment in the XY axis direction belongs to the prior art, it will not be repeated here.

[0026] In this embodiment, in order to achieve a better magnified imaging effect and a more stable microscopic shooting system, the imaging device is a microscope objective lens; in order to facilitate the assembly of lenses of shooting devices of different sizes and specifications with the microscope objective lens, the image acquisition system for microscopic objects also includes the following: Figure 1 The lens adapter ring 6 shown is used to connect the lens of the camera device to the objective lens of the microscope. Figure 1, the shooting device is a single-lens reflex viewfinder camera; the lens of the shooting device is a telephoto lens, specifically an infinity-lens SLR camera lens 5, such as a 200mm SLR camera telephoto lens, which is compatible with a microscope objective lens and can provide images with high resolution and high magnification; the infinity-lens SLR camera lens 5 is fixed on the single-lens reflex viewfinder camera body 3 through a camera lens fixing ring 4; the microscope objective lens is an infinity microscope objective lens with a certain magnification ratio, specifically a four-fold magnification infinity flat-field microscope objective lens 7; the infinity microscope objective lens and the infinity-lens SLR camera lens are combined into a high-magnification system with a four-fold magnification ratio, so that the details of the image collection object (such as microscopic objects such as stored grain insects) can be projected more clearly onto the photosensitive element of the SLR camera; and the four-fold magnification ratio infinity microscope objective lens can provide clear images of microscopic objects without causing image distortion or chromatic aberration.

[0027] In addition, refer to Figure 1 The stage also includes a mounting component for placing the image acquisition object and for fixing the image acquisition object relative to the rotating platform. Specifically, in order to be suitable for microscopic objects such as insect specimens, the mounting component is a specimen needle 10; a recessed portion is provided at the center position corresponding to the axial rotation of the rotating platform, and the mounting component is arranged in the recessed portion, which is perpendicular to the plane of the rotating platform, and the depth of the recessed portion is less than the height of the mounting component; wherein the recessed portion facilitates the fixed installation of the mounting component. The specimen needle used in this embodiment is 38 mm long and 0.27 mm in diameter. In other embodiments, mounting components of other types or specifications may also be used according to the actual image acquisition object.

[0028] Therefore, the specific method for collecting multi-view RGB images of microscopic objects used for three-dimensional modeling by the image collection system of microscopic objects in this embodiment is as follows:

[0029] Use a specimen needle with a diameter of 0.27mm to fix the microscopic object at the top of the needle tip, and then fix the specimen needle in the central concave part of the rotating platform, and adjust the angle and position so that it is perpendicular to the rotating platform plane and in the center of the platform. Then, adjust the XY axis adjustment seat of the stage and the position of the stage and the moving device as a whole to adjust the distance and position between the microscopic object sample and the SLR camera so that it can be clearly seen on the SLR camera screen; the specific position relationship is as follows Figure 3As shown by the arrow, the lens of the SLR camera and the connected microscope objective lens are aimed at the microscopic object (i.e., at the top position of the needle tip). Next, the continuous shooting mode of the SLR camera is turned on and the stepper motor is used to evenly control the focus distance of the SLR camera for shooting. An image is acquired every 0.01 mm movement, and 60 images are collected for each angle. Then the R-axis rotating platform is rotated around a circle, and the same operation is performed every 5° until the image acquisition is completed.

[0030] Since the focus depth of a high-magnification lens is narrow, only partially focused RGB images can be obtained. Therefore, multiple RGB images of different focus areas at the same angle are stacked, and multiple images are stacked together to obtain a fully focused image. Then, the SIFT feature matching algorithm is used to match the feature points of the stacked images, and then the images are corrected, segmented, and registered to eliminate image distortion, noise, and background interference, so that the complete morphology and characteristics of microscopic objects can be obtained.

[0031] The utility model has the following features:

[0032] 1) By means of an imaging device connected to the lens of the shooting device for magnifying and imaging, and a moving device capable of moving the image acquisition device forward and backward along the focal length change direction of the lens of the shooting device, the shooting device has a magnifying function similar to that of a microscope, thereby forming a microscopic photography system suitable for microscopic objects. At the same time, the lens focal length of the shooting device can be adjusted by the moving device to achieve focusing on different areas of the image acquisition object (i.e., the microscopic object), that is, focusing on different areas does not need to be achieved by moving the stage, thereby simplifying the operation of the moving stage when acquiring images of microscopic objects at different angles and different focus areas.

[0033] 2) By including a basic component for being stationary at a certain position and a slide rail sliding assembly for installing an image acquisition device and slidingly installing a sliding component relative to the basic component, it not only meets the requirement of moving the image acquisition device back and forth along the focal length change direction of the lens of the shooting device, but also ensures that the movement of the image acquisition device is limited to a certain range, thereby facilitating precise control of the moving position of the image acquisition device.

[0034] 3) By connecting with the mobile device to drive the mobile device to move step by step, and thus driving the image acquisition device to move step by step, the moving distance and moving frequency of the image acquisition device are automatically controlled. By taking a picture at each stepping movement, the focus depth of the camera can be automatically and evenly controlled to more efficiently obtain RGB images of different focus areas.

[0035] It should be understood that the above specific embodiments of the present invention are only used to exemplify or explain the principles of the present invention, and do not constitute a limitation to the present invention.

Claims

1. An image acquisition system for microscopic objects, comprising an image acquisition device, It is characterized in that The image acquisition device includes a shooting device and an imaging device for connecting to the lens of the shooting device and performing magnified imaging; it also includes a moving device for installing and moving the image acquisition device forward and backward along the focal length change direction of the lens; it also includes a stage; the stage includes a rotating platform; the rotating platform is used to place the image acquisition object and drive the image acquisition object to rotate axially, so as to expose different angles of the image acquisition object within the set field of view of the image acquisition device.

2. The image acquisition system for microscopic objects according to claim 1, It is characterized in that The moving device is a slide rail sliding assembly, which includes a basic part and a sliding part. The basic part is used to be stationary at a certain position, and the sliding part is used to install the image acquisition device and is slidably installed relative to the basic part to move the image acquisition device by sliding back and forth along the focal length change direction of the lens.

3. The image acquisition system for microscopic objects according to claim 1 or 2, It is characterized in that It also includes a stepping motor, which is used to connect with the moving device to drive the moving device to move in steps, thereby driving the image acquisition device to move in steps.

4. The image acquisition system for microscopic objects according to claim 1, It is characterized in that The stage also includes an XY axis adjustment seat, the rotating platform is mounted on the adjustment seat, and the adjustment seat is used to drive the rotating platform to change its position relative to the image acquisition device in space so as to adjust the position of the image acquisition object to within a set field of view of the image acquisition device; the plane formed by the XY axes is perpendicular to the axis direction of the axial rotation.

5. The image acquisition system for microscopic objects according to claim 1 or 2, It is characterized in that The imaging device is a microscope objective lens; the image acquisition system also includes a lens adapter ring for connecting the lens of the shooting device with the microscope objective lens.

6. The image acquisition system for microscopic objects according to claim 5, It is characterized in that The microscope objective lens is an infinite microscope objective lens with a certain magnification ratio.

7. The image acquisition system for microscopic objects according to claim 1 or 2, It is characterized in that The lens of the shooting device is a telephoto lens.

8. The image acquisition system for microscopic objects according to claim 1, It is characterized in that The stage also includes a mounting component for placing the image acquisition object and for fixing the image acquisition object relative to the rotating platform.

9. The image acquisition system for microscopic objects according to claim 8, It is characterized in that The mounting component is a specimen needle; a recessed portion is provided at the center position corresponding to the axial rotation of the rotating platform, the mounting component is arranged in the recessed portion, is perpendicular to the plane of the rotating platform, and the depth of the recessed portion is less than the height of the mounting component.

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