Filling device for cell culture medium

Abstract

The utility model relates to the technical field of cell culture media, and discloses a cell culture medium filling device which comprises a filling table, a material storage box is fixedly installed at the top of the filling table in a penetrating mode, a driving electric cylinder is fixedly installed at the bottom of the material storage box in an embedded mode, and a quantitative box is fixedly installed at the output tail end of the driving electric cylinder. The quantitative box and the material storage box are communicated through a corrugated pipe, a filling head is fixedly installed at one end of the bottom face of the quantitative box, a plugging assembly is installed in an inner cavity of the lower end of the filling head, a culture bottle is placed in a limiting ring, and the whole quantitative box can be driven to move downwards by starting a driving electric cylinder; and when the lower end of the filling head moves to the bottom of the inner cavity of the culture bottle, the blocking assembly is started, blocking of the L-shaped through hole by the concave blocking frame is relieved, the cell culture medium flows out of the bottom of the filling head into the culture bottle, filling is achieved, the cell culture medium is prevented from splashing, and use is convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture medium, in particular to a filling device for cell culture medium. Background Art

[0002] The source of stem cells is limited, the culture operation is complicated, and any slight change in the growth environment will affect the culture effect. In addition, the application of stem cells is infused according to the patient's kilogram body weight, and a large amount of expansion culture is required to meet the quantity requirements. During the cell culture process, the culture bottle needs to be filled with culture medium to ensure the cell culture environment. This process requires the use of a cell culture medium filling device.

[0003] For example, an automatic filling device for stem cell culture medium with publication number CN218232434U comprises a base plate and a storage box, wherein the storage box is arranged in the middle position above the base plate, a fixing mechanism is arranged on the base plate, the fixing mechanism comprises a fixing plate, a screw slider, a bidirectional screw, a connecting plate, a telescopic rod, a spring and a clamping plate, the fixing plate is symmetrically arranged at a position close to one end of the base plate, the screw slider is symmetrically arranged between the fixing plates, two ends of the bidirectional screw respectively pass through two screw sliders and are rotatably connected to the two fixing plates, the connecting plate is connected to one end of the screw slider, a plurality of telescopic rods are evenly arranged on one side of the connecting plate, one end of the plurality of telescopic rods is connected to the clamping plate, a spring is sleeved on the outer side of the telescopic rod, a feeding port is arranged on the storage box, and a stirring mechanism is arranged on the storage box. The utility model prevents the culture bottle from tipping over and the culture medium from settling during the filling process.

[0004] The above patent proposes that when the cell culture medium filling device is in use, the filling port is usually set just above the culture bottle, and the culture medium is filled into the culture bottle by spraying the culture medium. However, during the filling process, there is a distance between the filling port and the culture bottle, and the liquid culture medium is easy to splash around during filling, causing waste and inconvenience in use.

[0005] Therefore, we propose a filling device for cell culture medium to solve the above problems. Utility Model Content

[0006] The utility model aims to provide a filling device for cell culture medium, so as to solve the problem that during the filling process, there is a distance between the filling port and the culture bottle, and the liquid culture medium is easy to splash around during filling, thus causing waste.

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a filling device for cell culture medium, comprising a filling platform and feet fixedly installed at the four corners of the bottom surface of the filling platform, a material storage box is fixedly installed through the top of the filling platform, a driving electric cylinder is embedded and fixedly installed at the bottom of the material storage box, a quantitative box is fixedly installed at the output end of the driving electric cylinder, the quantitative box and the material storage box are connected by a bellows, and the bellows is arranged at one end of the quantitative box, a filling head is fixedly installed at one end of the bottom surface of the quantitative box, a sealing assembly is installed in the inner cavity at the lower end of the filling head, a quantitative piston is installed at the other end of the quantitative box, and a control console is fixedly installed on the front outer wall of the filling platform.

[0008] Preferably, a filling groove is provided at the upper end of the front side of the filling platform, and a limiting ring is fixedly installed on the bottom surface of the inner cavity of the filling groove.

[0009] Preferably, a feeding port is connected and fixedly installed on the outer wall of the storage box.

[0010] Preferably, the quantitative box comprises a box body and a connecting protrusion fixedly mounted on the outer wall of the middle portion of one end of the box body, and the output end of the driving electric cylinder is fixedly mounted on the outer wall of the connecting protrusion.

[0011] Preferably, a threaded hole is connected to the middle outer wall of the other end of the box body, an observation hole is provided on the outer wall of one side of the box body, a transparent plate is fixedly installed in the inner cavity of the observation hole, and a scale line is provided at the upper end of the outer side of the observation hole.

[0012] Preferably, the metering piston comprises a threaded column and a piston plate rotatably mounted on the front end of the threaded column, and a screwing head is fixedly mounted on one end of the threaded column.

[0013] Preferably, a feed trough is provided in the middle of the top surface of the filling head, a bottom through hole is provided in the middle of the bottom surface of the inner cavity of the feed trough, inner sliding grooves are provided on the inner walls at both ends of the inner cavity of the bottom through hole, and L-shaped through holes are provided on the inner walls at both ends of the lower end of the inner cavity of the feed trough.

[0014] Preferably, the blocking assembly includes an upper push column and a concave blocking frame fixedly installed on the upper end of the upper push column, and sliders are fixedly installed on the outer walls at both ends of the upper push column, and the sliders are elastically slidably arranged in the inner slide groove by springs.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] 1. When cell culture medium needs to be added, the culture bottle can be placed in the limit ring. By starting the driving electric cylinder, the quantitative box can be driven to move downward as a whole until the lower end of the filling head moves to the bottom of the inner cavity of the culture bottle. At this time, the bottom of the push-up column will first contact the bottom of the inner cavity of the culture bottle and be pushed upward at the same time, so that the push-up column drives the concave sealing frame to move upward together, and the concave sealing frame releases the blockage of the L-shaped through hole, so that the cell culture medium flows out from the bottom of the filling head into the culture bottle, and the filling is realized, which prevents the cell culture medium from splashing and is convenient for use.

[0017] 2. When the amount of culture medium to be added to the culture bottle is different, the screw head can be screwed, and the threaded column will drive the piston plate to move back and forth in the inner cavity of the box, thereby changing the volume of the cavity between the piston plate and the inner cavity of the box, and reading can be made through the scale line, so it can be suitable for filling different amounts of culture medium. It is not only convenient and quick to operate, but also improves the adaptability of the filling device. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0019] Figure 2 It is a schematic diagram of the three-dimensional structure of the filling station of the utility model;

[0020] Figure 3 This is a schematic diagram of the connection structure of the material storage box, the driving electric cylinder and the quantitative box of the utility model;

[0021] Figure 4 This is a schematic diagram of the three-dimensional structure of the quantitative box of the utility model;

[0022] Figure 5 This is a schematic diagram of the disassembled structure of the filling head and the plugging assembly of the utility model;

[0023] Figure 6 It is a schematic diagram of the split structure of the quantitative piston of the utility model.

[0024] In the figure: 1. filling platform; 11. filling slot; 12. limit ring; 2. standing foot; 3. storage box; 31. feeding port; 4. driving electric cylinder; 5. quantitative box; 51. box body; 511. threaded hole; 512. observation through hole; 513. transparent plate; 514. scale line; 52. connecting protrusion; 6. bellows; 7. quantitative piston; 71. threaded column; 711. screw head; 72. piston plate; 8. filling head; 81. feeding slot; 82. bottom through hole; 83. inner slide groove; 84. L-shaped through hole; 9. plugging assembly; 91. push-up column; 92. concave plugging frame; 93. slider; 94. spring; 10. control console. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] Example 1: Please refer to Figure 1-5 , a filling device for cell culture medium, comprising a filling platform 1 and standing feet 2 fixedly installed at the four corners of the bottom surface of the filling platform 1, characterized in that: a storage box 3 is fixedly installed on the top of the filling platform 1, a driving electric cylinder 4 is embedded and fixedly installed on the bottom of the storage box 3, and a quantitative box 5 is fixedly installed on the output end of the driving electric cylinder 4, and the quantitative box 5 and the storage box 3 are connected through a bellows 6. At the same time, an electric valve is provided at the connection between the storage box 3 and the bellows 6, which is used to control the discharge of the culture medium in the storage box 3 and the suspension of the discharge. This is the prior art and no further description is needed. The bellows 6 is provided at one end of the quantitative box 5, and a filling head 8 is fixedly installed at one end of the bottom surface of the quantitative box 5, and a blocking component 9 is installed in the inner cavity at the lower end of the filling head 8, and a quantitative piston 7 is installed at the other end of the quantitative box 5, and a control console 10 is fixedly installed on the front outer wall of the filling platform 1.

[0027] A filling groove 11 is arranged at the upper end of the front side of the filling platform 1 , and a limiting ring 12 is fixedly installed on the bottom surface of the inner cavity of the filling groove 11 , and the limiting ring 12 is used to locate the position of the culture bottle.

[0028] A feeding port 31 is fixedly mounted on the outer wall of the storage box 3 . By providing the feeding port 31 , the cell culture medium can be added to the storage box 3 .

[0029] The quantitative box 5 includes a box body 51 and a connecting protrusion 52 fixedly mounted on the outer wall of the middle part of one end of the box body 51 , and the output end of the driving electric cylinder 4 is fixedly mounted on the outer wall of the connecting protrusion 52 .

[0030] A feed trough 81 is provided in the middle of the top surface of the filling head 8, a bottom through hole 82 is provided in the middle of the bottom surface of the inner cavity of the feed trough 81, inner sliding grooves 83 are respectively provided on the inner walls at both ends of the inner cavity of the bottom through hole 82, and L-shaped through holes 84 are respectively provided on the inner walls at both ends of the lower end of the inner cavity of the feed trough 81, and the lower ends of the L-shaped through holes 84 are respectively connected to the bottom surface of the filling head 8.

[0031] The sealing assembly 9 includes an upper push column 91 and a concave sealing frame 92 fixedly installed on the upper end of the upper push column 91. Sliders 93 are fixedly installed on the outer walls of both ends of the upper push column 91, and the slides 93 are elastically slidably set in the inner slide groove 83 by springs 94. In the initial state, the concave sealing frame 92 blocks the L-shaped through hole 84 respectively.

[0032] In this embodiment: when cell culture medium needs to be added, the culture bottle can be placed in the limiting ring 12, and by starting the driving electric cylinder 4, the quantitative box 5 can be driven to move downward as a whole until the lower end of the filling head 8 moves to the bottom of the inner cavity of the culture bottle. At this time, the bottom of the push-up column 91 will first contact the bottom of the inner cavity of the culture bottle, and be pushed upward at the same time, so that the push-up column 91 drives the concave sealing frame 92 to move upward together, and the concave sealing frame 92 releases the blockage of the L-shaped through hole 84, so that the cell culture medium flows out from the bottom of the filling head 8 into the culture bottle, so as to achieve filling, prevent the cell culture medium from splashing, and facilitate use.

[0033] Furthermore, by setting the spring 94, when the blocking component 9 moves upward, the concave blocking frame 92 will automatically block the L-shaped through hole 84, so as to carry out the next filling operation, which is convenient to use.

[0034] Embodiment 2: This embodiment is an improvement made on the basis of embodiment 1. For details, please refer to Figure 3 , Figure 4 and Figure 6 A threaded hole 511 is provided on the outer wall in the middle of the other end of the box body 51, and an observation hole 512 is provided on the outer wall of one side of the box body 51. A transparent plate 513 is fixedly installed in the inner cavity of the observation hole 512, and a scale line 514 is provided at the upper end of the outer side of the observation hole 512. The staff can adjust the inner cavity volume of the box body 51 through the scale line 514 and the quantitative piston 7.

[0035] The metering piston 7 includes a threaded column 71 and a piston plate 72 rotatably mounted on the front end of the threaded column 71, and the threaded column 71 is threadedly penetrated in the threaded hole 511, and the piston plate 72 is sealingly slidably arranged in the inner cavity of the box body 51, and a screwing head 711 is fixedly mounted on one end of the threaded column 71, and a plurality of V-shaped grooves are arranged on the outer peripheral surface of the screwing head 711 for increasing the friction between the fingers.

[0036] In this embodiment: when the amount of culture medium that needs to be added to the culture bottle is different, the screw head 711 can be screwed, and the screw head 711 will drive the threaded column 71 to rotate. Under the restriction of the threaded hole 511, the threaded column 71 will drive the piston plate 72 to move back and forth in the inner cavity of the box body 51, thereby changing the cavity volume between the piston plate 72 and the inner cavity of the box body 51, and can be read through the scale line 514, so that it can be suitable for filling different amounts of culture medium. It is not only convenient and quick to operate, but also improves the adaptability of the filling device.

[0037] The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

[0038] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art may still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A filling device for cell culture medium, comprising a filling platform (1) and feet (2) respectively fixedly mounted at four corners of the bottom surface of the filling platform (1), Features: A material storage box (3) is fixedly installed through the top of the filling platform (1), a driving electric cylinder (4) is embedded and fixedly installed in the bottom of the material storage box (3), a quantitative box (5) is fixedly installed at the output end of the driving electric cylinder (4), the quantitative box (5) and the material storage box (3) are connected through a bellows (6), and the bellows (6) is arranged at one end of the quantitative box (5), a filling head (8) is fixedly installed at one end of the bottom surface of the quantitative box (5), a blocking component (9) is installed in the lower end inner cavity of the filling head (8), a quantitative piston (7) is installed at the other end of the quantitative box (5), and a control console (10) is fixedly installed on the front outer wall of the filling platform (1).

2. A cell culture medium filling device according to claim 1, Features: A filling groove (11) is provided at the upper end of the front face of the filling platform (1), and a limiting ring (12) is fixedly mounted on the bottom surface of the inner cavity of the filling groove (11).

3. A cell culture medium filling device according to claim 2, Features: A feeding port (31) is fixedly mounted on the outer wall of the material storage box (3).

4. A filling device for cell culture medium according to claim 3, Features: The quantitative box (5) comprises a box body (51) and a connecting protrusion (52) fixedly mounted on the outer wall of the middle portion of one end of the box body (51), and the output end of the driving electric cylinder (4) is fixedly mounted on the outer wall of the connecting protrusion (52).

5. A filling device for cell culture medium according to claim 4, Features: A threaded hole (511) is provided on the outer wall in the middle of the other end of the box body (51), an observation through hole (512) is provided on the outer wall of one side of the box body (51), a transparent plate (513) is fixedly installed in the inner cavity of the observation through hole (512), and a scale line (514) is provided at the upper end of the outer side of the observation through hole (512).

6. A filling device for cell culture medium according to claim 5, Features: The quantitative piston (7) comprises a threaded column (71) and a piston plate (72) rotatably mounted on the front end of the threaded column (71); a screwing head (711) is fixedly mounted on one end of the threaded column (71).

7. A cell culture medium filling device according to claim 6, Features: A feed groove (81) is provided in the middle of the top surface of the filling head (8), a bottom through hole (82) is provided in the middle of the bottom surface of the inner cavity of the feed groove (81), inner sliding grooves (83) are provided on the inner walls at both ends of the inner cavity of the bottom through hole (82), and L-shaped through holes (84) are provided on the inner walls at both ends of the lower end of the inner cavity of the feed groove (81).

8. A cell culture medium filling device according to claim 7, Features: The blocking assembly (9) comprises an upper push column (91) and a concave blocking frame (92) fixedly mounted on the upper end of the upper push column (91); sliding blocks (93) are fixedly mounted on the outer walls at both ends of the upper push column (91), and the sliding blocks (93) are elastically slidably arranged in the inner slide groove (83) via springs (94).

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