Sampling device for stainless steel cell reactor

Abstract

The utility model discloses a sampling device for a stainless steel cell reactor. According to the sampling device for the stainless steel cell reactor, the inlet end of a first main pipeline is used for being connected into the stainless steel cell reactor; the first valve is connected to the first main pipeline; the outlet end of the first main pipeline is connected into the buffer bottle; the ventilation pipeline is connected to the buffer bottle; the inlet end of the second main pipeline is connected into the buffer bottle; the outlet end of the second main pipeline is used for connecting a sampling bottle; the second valve is connected to the second main pipeline; the branch pipeline is connected to the first main pipeline; the branch pipeline is used for introducing gas to push the cell sap in the first main pipeline to enter the buffer bottle; the first air filter is connected to the branch pipeline; the third valve is connected to the branch pipeline; and the blow-off pipeline is connected to the first main pipeline. Compared with the prior art, steam flushing before and after sampling each time is avoided, and electric energy consumption and steam emission are reduced.

Description

Technical Field

[0001] The utility model relates to an experimental device, in particular to a sampling device used for a stainless steel cell reactor. Background Art

[0002] The existing stainless steel cell reactor has a tank body made of 316L stainless steel and a double-layer tank wall jacket for temperature control. High-temperature and high-pressure steam is often used for sterilization before cell culture. In laboratories and production areas with low environmental requirements, the stainless steel sampling pipe port is also sterilized by steam flushing before and after each sampling. There is tank pressure during the cultivation process of the stainless steel reactor. After the sampling port is sterilized, the cell culture fluid is discharged by relying on the tank pressure.

[0003] If the sampling port is sterilized by steam, it needs to be flushed for at least 10 minutes before sampling. After the steam is turned off, the pipe needs to wait for a while to cool down or the cell fluid with more loss needs to be cooled before formal sampling can be carried out. After sampling is completed, the sampling port needs to be flushed with steam. The steam discharged before and after flushing and the cell fluid flushed after sampling may have an adverse effect on the air environment of the laboratory or production room with higher requirements. The sampling operation will be repeated many times during the culture process. The tank volume is not large and the number is small. The cell reactor with centralized steam supply is not used. Because the matching steam generator is frequently opened for sampling, it brings inconvenience and increases energy consumption. Utility Model Content

[0004] The utility model aims to provide a sampling device for a stainless steel cell reactor, which avoids steam elimination before and after each sampling, reduces power consumption and steam emission, and reduces the impact on laboratories or production areas with high environmental requirements.

[0005] In order to solve the above technical problems, the embodiment of the utility model provides a sampling device for a stainless steel cell reactor, comprising:

[0006] A first main pipeline, wherein the inlet end of the first main pipeline is used to connect to a stainless steel cell reactor;

[0007] a first valve connected to a first main pipe;

[0008] A buffer bottle, the outlet end of the first main line is connected to the buffer bottle;

[0009] A vent pipe connected to the buffer bottle;

[0010] a second main line, the inlet end of which is connected to the buffer bottle and is used to obtain the cell fluid flowing into the buffer bottle from the first main line; the outlet end of the second main line is used to connect to a sampling bottle;

[0011] a second valve, the second valve being connected to the second main pipe;

[0012] A branch pipeline, the branch pipeline is connected to the first main pipeline and is located between the first valve and the outlet end of the first main pipeline; the branch pipeline is used to pass gas to push the cell fluid in the first main pipeline into the buffer bottle;

[0013] A first air filter connected to the branch pipe;

[0014] a third valve connected to the branch line and located between the first air filter and the first main line; and

[0015] A sewage discharge pipeline is connected to the first main pipeline.

[0016] Compared with the prior art, the embodiment of the utility model, during the sampling operation, introduces gas through the branch pipeline to push the cell fluid in the first main pipeline and the second main pipeline to be discharged from the outlet end of the second main pipeline, so that the cell fluid in the first main pipeline and the second main pipeline is basically discharged, so that there is no need for steam flushing before and after each time, which reduces power consumption and steam emissions, and reduces the impact on laboratories or production areas with high environmental requirements. In addition, after sampling, the first main pipeline can be naturally ventilated or air intake can be applied through the first air filter to better empty the first main pipeline, reducing the residue in the first main pipeline.

[0017] In one embodiment, the buffer bottle comprises a bottle body and a bottle cap for sealing the bottle body;

[0018] The first main line is bent and inserted into the bottle cap to enter the bottle body, and the outlet end of the first main line and the air-permeable pipe are both located at the top inside the bottle body; the second main line is bent and inserted into the bottle cap to enter the bottle body, and the inlet end of the second main line is located at the bottom inside the bottle body.

[0019] In one embodiment, the distance from the branch line to the first valve is shorter than the distance to the buffer bottle.

[0020] In one embodiment, the ratio of the distance from the branch line to the first valve to the distance from the branch line to the buffer bottle is in a range of 1:10 to 1:20.

[0021] In one embodiment, a fourth valve and a second air filter are connected to the air permeable pipeline, and the fourth valve is located between the second air filter and the buffer bottle.

[0022] In one embodiment, the first valve is a three-way diaphragm valve connected to the sewage pipeline;

[0023] The air-permeable pipeline, the branch pipeline and the second main pipeline are all silicone pipelines, and the second valve, the third valve and the fourth valve are all pipe clamps.

[0024] In one embodiment, the second main line has an inlet pipe portion inserted into the bottom of the buffer bottle, a connecting pipe portion, and an outlet pipe portion inserted into the sampling bottle;

[0025] The inlet pipe portion and the outlet pipe portion are bent and extended downward from the connecting pipe portion, and the second valve is connected to the connecting pipe portion.

[0026] In one embodiment, alcohol is placed in the sampling bottle, and the outlet tube is inserted into the alcohol.

[0027] In one embodiment, the first main pipe and the connecting pipe portion extend in the same direction.

[0028] In one embodiment, the sewage pipeline is connected between the first valve and the inlet end of the first main pipeline, and a fifth valve is connected to the sewage pipeline. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a structural schematic diagram of a sampling device in an embodiment of the utility model;

[0030] Description of the drawings: 100, sampling device; 200, sampling bottle; 1, first main line; 2, first valve; 3, buffer bottle; 31, bottle body; 32, bottle cap; 4, ventilation line; 5, second main line; 16, inlet pipe; 17, connecting pipe; 18, outlet pipe; 6, second valve; 7, branch line; 8, first air filter; 9, third valve; 10, sewage line; 11, fifth valve; 14, fourth valve; 15, second air filter; 19, inlet end of the first main line; 20, outlet end of the first main line; 21, inlet end of the second main line; 22, outlet end of the second main line. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical scheme and advantages of the utility model clearer, the various embodiments of the utility model will be described in detail below with reference to the accompanying drawings. However, it can be understood by those skilled in the art that in the various embodiments of the utility model, many technical details are provided in order to enable readers to better understand the present application. However, even without these technical details and various changes and modifications based on the following embodiments, the technical schemes claimed for protection in the claims of the present application can be implemented.

[0032] Unless the context requires otherwise, throughout the specification and claims, the word "comprise" and variations such as "include" and "have" should be construed in an open, inclusive sense, ie, should be interpreted as "including, but not limited to."

[0033] The following will be combined with the accompanying drawings to describe the various embodiments of the present invention in detail, so as to more clearly understand the purpose, characteristics and advantages of the present invention. It should be understood that the embodiments shown in the accompanying drawings are not intended to limit the scope of the present invention, but are only intended to illustrate the essential spirit of the technical solution of the present invention.

[0034] References throughout the specification to "one embodiment" or "an embodiment" indicate that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of "in one embodiment" or "in an embodiment" in various places throughout the specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any manner in one or more embodiments.

[0035] As used in this specification and the appended claims, the singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise. It should be noted that the term "or" is generally employed in its sense including "and / or" unless the context clearly dictates otherwise.

[0036] In the following description, in order to clearly demonstrate the structure and working mode of the utility model, many directional words will be used for description, but the words "front", "back", "left", "right", "outside", "inside", "outward", "inward", "up", "down", etc. should be understood as convenient terms and should not be understood as restrictive terms.

[0037] Embodiments of the present invention are described below with reference to the accompanying drawings.

[0038] An embodiment of the present invention relates to a sampling device 100 for a stainless steel cell reactor. Figure 1As shown, the sampling device 100 for the stainless steel cell reactor includes: a first main line 1, a first valve 2, a buffer bottle 3, a breathable line 4, a second main line 5, a second valve 6, a branch line 7, a first air filter 8, a third valve 9 and a sewage line 10. The inlet end 19 of the first main line 1 is used to access the stainless steel cell reactor, and the inlet end 19 of the first main line 1 can be connected to the stainless steel cell reactor through a stainless steel line. The first valve 2 is connected to the first main line 1, the outlet end 20 of the first main line 1 is connected to the buffer bottle 3, and the breathable line 4 is connected to the buffer bottle 3. The inlet end 21 of the second main line 5 is connected to the buffer bottle 3, for obtaining the cell fluid flowing from the first main line 1 into the buffer bottle 3, and the outlet end 22 of the second main line 5 is used to access the sampling bottle 200. The second valve 6 is connected to the second main line 5. The branch line 7 is connected to the first main line 1 and is located between the first valve 2 and the outlet end 20 of the first main line 1. The branch line 7 is used to pass gas to push the cell fluid in the first main line 1 into the buffer bottle 3, and the gas can enter from the direction of arrow A. The third valve 9 and the first air filter 8 are connected to the branch line 7. The third valve 9 is located between the first air filter 8 and the first main line 1. The sewage line 10 is connected to the first main line 1.

[0039] During the sampling operation, the gas is introduced through the branch line 7 to push the cell fluid of the first main line 1 and the second main line 5 to be discharged from the outlet end 22 of the second main line 5, so that the cell fluid in the first main line and the second main line 5 is basically discharged, so that there is no need for steam elimination before and after each time, which reduces the power consumption and steam emission, and reduces the impact on laboratories or production areas with high environmental requirements. In addition, after sampling, the first main line 1 can be better emptied through the first air filter 8 for natural ventilation or air intake, reducing the residue of the first main line 1. The prior art has only one main line connecting the stainless steel organelle and the sampling bottle 200. When sampling, air will directly enter the main line, thereby affecting the main line. The buffer bottle 3 reduces the risk of backflow contamination caused by the exposure of the inlet end 21 of the second main line 5. That is, because the second main line 5 is inserted into the sampling bottle 200, when air enters the inlet end 21 of the second main line 5, it is discharged into the buffer bottle 3, and will not directly interfere with the main line like in the prior art.

[0040] In addition, if Figure 1 As shown, the sewage pipeline 10 is connected between the first valve 2 and the inlet end 19 of the first main pipeline 1, and a fifth valve 11 is connected to the sewage pipeline 10. The connection of the sewage pipeline 10 needs to be set before the closure of the diaphragm at the inlet end 19 of the first main pipeline 1, eliminating dead angles as much as possible, and sterilizing and exhausting the pipeline connected between the sampling device 100 and the stainless steel cell reactor.

[0041] Furthermore, if Figure 1As shown, the buffer bottle 3 includes a bottle body 31 and a bottle cap 32 for sealing the bottle body 31. The first main line 1 is bent and inserted into the bottle cap 32 to enter the bottle body 31, and the outlet end 20 of the first main line 1 and the air permeable pipe 4 are both located at the top of the bottle body 31; the second main line 5 is bent and inserted into the bottle cap 32 to enter the bottle body 31, and the inlet end 21 of the second main line 5 is located at the bottom of the bottle body 31.

[0042] Furthermore, if Figure 1 As shown, the distance from the branch line 7 to the first valve 2 is less than the distance to the buffer bottle 3. Preferably, the distance from the branch line 7 to the first valve 2 is in the range of 1:10 to 1:20 to the distance from the branch line 7 to the buffer bottle 3. The connection position of the branch line 7 is closer to the first valve 2 to reduce the dead volume before the branch line 7. After sampling, the first air filter 8 can be naturally ventilated or air is applied to better empty the pipeline of the sampling device 100, reducing pipeline residue.

[0043] Furthermore, if Figure 1 As shown, the ventilation pipeline 4 is connected to a fourth valve 14 and a second air filter 15 , and the fourth valve 14 is located between the second air filter 15 and the buffer bottle 3 .

[0044] Furthermore, if Figure 1 As shown, the first valve 2 is a three-way diaphragm valve, which connects the sewage pipeline 10, the main pipeline, and the branch pipeline 7. The vent pipeline 4, the branch pipeline 7 and the second main pipeline 5 are all silicone pipelines, and the second valve 6, the third valve 9 and the fourth valve 14 are all pipe clamps. In addition to Robert water stop clamps, the second valve 6, the third valve 9 and the fourth valve 14 can also use other hose water stop clamps, hemostatic forceps and other pipe clamps or valves and other accessories suitable for the pipelines of the device.

[0045] Specifically, if Figure 1 As shown, the second main line 5 has an inlet pipe portion 16 inserted into the bottom of the buffer bottle 3, a connecting pipe portion 17, and an outlet pipe portion 18 inserted into the sampling bottle 200. The inlet pipe portion 16 and the outlet pipe portion 18 are bent and extended downward by the connecting pipe portion 17, and the second valve 6 is connected to the connecting pipe portion 17.

[0046] Furthermore, if Figure 1 As shown, the first main pipeline 1 and the connecting pipe portion 17 extend in the same direction.

[0047] In addition, if Figure 1 As shown, alcohol is placed in the sampling bottle 200, and the outlet tube portion 18 is inserted into the alcohol.

[0048] In addition, if Figure 1 As shown, the first air filter 8 and the second air filter 15 are both 0.2um sterilizable air filters.

[0049] like Figure 1 As shown, when the sampling device 100 is used normally, the flow direction of the cell fluid should be as shown by arrow B, entering from the inlet end 19 of the first main line 1, passing through the first main line 1, the first valve 2, the buffer bottle 3, the second main line 5, and finally flowing out from the outlet end 22 of the second main line 5 in the sampling bottle 200, that is: first main line 1→first valve 2→buffer bottle 3→second main line 5→sampling bottle 200.

[0050] like Figure 1 As shown, after assembling the sampling device 100, it needs to be fully sterilized to maintain a sterile environment inside the pipeline that the cell fluid may contact. The chuck interface of the first main pipeline 1 and the sewage pipeline 10 is wrapped with kraft paper, multi-layer dust-free cloth or other sterilization wrapping materials, the first valve 2, the fifth valve 11, the third valve 9, the second valve 6 and the fourth valve 14 are opened, and the sampling bottle 200 is dry and free of matter. The whole set of sampling device 100 is sterilized. After the sterilization is completed, the first valve 2, the fifth valve 11 and the second valve 6 need to be closed, and the sampling device 100 is cooled and ready for use. During the culture process, it is necessary to ensure the sterile use of the cell culture fluid from the stainless steel cell reactor, through the sampling valve and the connection, to the entire pipeline of the sampling device 100. After the sterilized sampling device 100 is docked with the internal environment of the stainless steel cell reactor where the cell fluid is located, the connection with the cell reactor must be fully sterilized before sampling. The first main pipeline 1 in the assembled and sterilized sampling device 100 is sealed and connected to the sampling pipeline of the stainless steel cell reactor by means of a chuck or a silicone tube, and the external sewage pipe is connected to the sewage pipeline 10. If there is no external sewage pipe available, a waste liquid cup or a waste liquid bottle can be placed under the outlet end of the sewage pipeline 10, and a single open drainage is adopted. The steam pipeline of the stainless steel cell reactor is opened, and the sampling port of the stainless steel cell reactor and the connection part of the sampling device 100 are sterilized. This operation can be performed together with the steam sterilization of the stainless steel cell reactor itself. After the sterilization, the fifth valve 11 is closed to ensure that the sterile environment in the first main pipeline 1 is separated from the sewage pipe.

[0051] Specifically, Figure 1As shown, after the sampling device 100 is correctly connected and the connection is sterilized, sampling can be performed normally. First, before starting sampling, ensure that the third valve 9 is closed, the fourth valve 14 is open, and the second valve 6 is closed; second, open the stainless steel cell reactor sampling valve, open the first valve 2, and the cell culture fluid will flow out to the buffer bottle 3 under the action of its own tank pressure. After a certain amount of flow out, close the sampling valve and the first valve 2; third, close the fourth valve 14, take the outlet end 22 of the second main line 5 out of the sampling bottle 200 alcohol, and place it in a clean and sterilized sampling tube / bottle; fourth, open the second valve 6, and at the same time apply the intake pressure from the filter outside the branch line 7 to discharge the cell culture fluid in the first main line 1 and the buffer bottle 3 into the second main line 5 and the sampling bottle 200. After the pipeline is emptied, close the second valve 6 and stop the intake of the branch line 7; fifth, repeat steps one to four once, control the amount of the previous sampling, and use it to flush the pipeline residue before sampling. The second formal sampling is used for cell culture fluid sample detection; sixth, put the outlet end 22 of the second main line 5 back into the sampling bottle 200 filled with 75% alcohol. Repeat the above operation for the next sampling, without disassembly for cleaning or steam sterilization. The 75% alcohol at the end of the sampling bottle 200 should be replaced regularly, and if possible, after each sampling.

[0052] Preferred embodiments of the present invention have been described in detail above, but it should be understood that aspects of the embodiments can be modified, if necessary, to employ aspects, features and concepts of the various patents, applications and publications to provide further embodiments.

[0053] These and other changes can be made to the embodiments in light of the above detailed description.In general, in the claims, the terms used should not be considered limited to the specific embodiments disclosed in the specification and the claims, but should be construed to include all possible embodiments along with the full scope of equivalents to which these claims are entitled.

[0054] Those skilled in the art will appreciate that the above-mentioned embodiments are specific examples for implementing the present invention, and in actual applications, various changes may be made thereto in form and detail without departing from the spirit and scope of the present invention.

Claims

1. A sampling device for a stainless steel cell reactor, It is characterized in that include: A first main pipeline, wherein the inlet end of the first main pipeline is used to connect to a stainless steel cell reactor; a first valve connected to a first main pipe; A buffer bottle, the outlet end of the first main line is connected to the buffer bottle; A vent pipe connected to the buffer bottle; a second main line, the inlet end of which is connected to the buffer bottle and is used to obtain the cell fluid flowing into the buffer bottle from the first main line; the outlet end of the second main line is used to connect to a sampling bottle; a second valve, the second valve being connected to the second main pipe; A branch pipeline, the branch pipeline is connected to the first main pipeline and is located between the first valve and the outlet end of the first main pipeline; the branch pipeline is used to pass gas to push the cell fluid in the first main pipeline into the buffer bottle; A first air filter connected to the branch pipe; a third valve connected to the branch line and located between the first air filter and the first main line; and A sewage discharge pipeline is connected to the first main pipeline.

2. The sampling device for a stainless steel cell reactor according to claim 1, It is characterized in that The buffer bottle comprises a bottle body and a bottle cap for sealing the bottle body; The first main line is bent and inserted into the bottle cap to enter the bottle body, and the outlet end of the first main line and the air-permeable pipe are both located at the top inside the bottle body; the second main line is bent and inserted into the bottle cap to enter the bottle body, and the inlet end of the second main line is located at the bottom inside the bottle body.

3. The sampling device for a stainless steel cell reactor according to claim 1, It is characterized in that The distance from the branch line to the first valve is shorter than the distance to the buffer bottle.

4. The sampling device for a stainless steel cell reactor according to claim 3, It is characterized in that The ratio of the distance from the branch line to the first valve to the distance from the branch line to the buffer bottle is in a range of 1:10 to 1:

20.

5. The sampling device for a stainless steel cell reactor according to claim 1, It is characterized in that The ventilation pipeline is connected with a fourth valve and a second air filter, and the fourth valve is located between the second air filter and the buffer bottle.

6. The sampling device for a stainless steel cell reactor according to claim 5, It is characterized in that The first valve is a three-way diaphragm valve connected to the sewage pipeline; The air-permeable pipeline, the branch pipeline and the second main pipeline are all silicone pipelines, and the second valve, the third valve and the fourth valve are all pipe clamps.

7. The sampling device for a stainless steel cell reactor according to claim 1, It is characterized in that The second main pipe has an inlet pipe portion inserted into the bottom of the buffer bottle, a connecting pipe portion, and an outlet pipe portion inserted into the sampling bottle; The inlet pipe portion and the outlet pipe portion are bent and extended downward from the connecting pipe portion, and the second valve is connected to the connecting pipe portion.

8. The sampling device for a stainless steel cell reactor according to claim 7, It is characterized in that Alcohol is placed in the sampling bottle, and the outlet pipe portion is inserted into the alcohol.

9. The sampling device for a stainless steel cell reactor according to claim 7, It is characterized in that The first main pipe and the connecting pipe portion extend in the same direction.

10. The sampling device for a stainless steel cell reactor according to claim 1, It is characterized in that The sewage discharge pipeline is connected between the first valve and the inlet end of the first main pipeline, and a fifth valve is connected to the sewage discharge pipeline.

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