Antibody treatment equipment

Abstract

The utility model discloses antibody treatment equipment which comprises an enzyme digestion system and a purification system, the enzyme digestion system comprises a heating mechanism, a reaction mechanism and a rotatable mechanism used for being connected with a reaction tube, the reaction mechanism comprises a reaction tank, and the heating mechanism is arranged on the periphery of the reaction tank; the purification system comprises a purification mechanism and a magnetic part; the purification mechanism comprises a purification tank; the magnetic part is arranged at one end of the purification tank and is used for purifying a to-be-purified substance in the purification tank. The treatment equipment is small in size and low in manufacturing cost, the reaction temperature can be strictly controlled, enzyme digestion Fab fragments can be efficiently and rapidly obtained, meanwhile, the difference between batches is reduced, and the stability of the batches is guaranteed.

Description

Technical Field

[0001] The utility model relates to the field of antibody processing, in particular to an antibody processing device. Background Art

[0002] Fab fragment (Antigen-binding fragment), also known as antigen-binding fragment, is the region in the antibody structure that can bind to the antigen. Fab has both the antigen-binding region and part of the constant region, which not only enables it to have the same antibody-antigen affinity and excellent tissue penetration as single-chain antibody (scFv), but also has a more stable structure, thus playing a huge role in clinical diagnosis and treatment.

[0003] At present, the method for preparing Fab antibodies is mainly divided into two steps. The first step is enzymatic hydrolysis, which uses pepsin, papain, etc. to degrade immunoglobulin G into products such as F(ab')2, Fab, and Fc fragments. The second step is to separate and purify by affinity chromatography, ion exchange chromatography, and gel filtration to remove Fc and uncut immunoglobulins to obtain Fab antibody products. It can be seen that the existing process for preparing Fab antibodies involves multiple experimental operations, and the reaction conditions of each experiment cannot be fixed, resulting in different enzymatic efficiency and purification efficiency. There are large differences between each batch, and it is not very stable as a diagnostic reagent raw material.

[0004] In addition, most of the devices currently published for enzymatic cleavage of proteins or antibodies are large-scale bioreactors used to prepare raw materials. They are expensive and have batch differences, and are unable to purify the reactants after the enzymatic cleavage reaction.

[0005] In view of this, this application is hereby filed. Utility Model Content

[0006] The utility model aims to provide an antibody processing device, which has small size, low cost and high efficiency, and ensures the stability between batches while quickly obtaining enzyme-cut Fab fragments.

[0007] The embodiment of the utility model is achieved as follows:

[0008] The antibody processing equipment provided by the utility model comprises an enzyme cutting system and a purification system; the enzyme cutting system comprises a heating mechanism, a reaction mechanism and a rotatable mechanism for connecting a reaction tube, the reaction mechanism comprises a reaction tank, the heating mechanism is arranged on the periphery of the reaction tank, and the rotatable mechanism is arranged at the bottom of the reaction tank; the purification system comprises a purification mechanism and a magnetic part, the purification mechanism comprises a purification tank; the magnetic part is arranged at one end of the purification tank, and the magnetic part is used to purify the substance to be purified in the purification tank.

[0009] In an optional embodiment, the purification system further includes a second reaction tube; the second reaction tube is used to hold the substance to be purified; and the second reaction tube is used in combination with the purification tank.

[0010] In an optional embodiment, the processing equipment also includes a control device; the control device is connected to the heating element of the heating mechanism and controls the switching and temperature of the heating element; the control device is also connected to the rotatable mechanism to control the switching and rotation of the rotatable mechanism.

[0011] In an optional embodiment, the heating mechanism includes a heating element, which has an annular structure and extends from the top to the bottom of the reaction tank; a heat conductive medium is placed between the heating element and the reaction tank, and the outer side of the heating element is made of a heat insulating material.

[0012] In an optional embodiment, the groove wall and the groove bottom of the reaction groove are both made of heat-conductive material; the inner side of the groove wall of the reaction groove is provided with ridges / grooves; and the ridges / grooves are arranged along the axial direction of the reaction groove.

[0013] In an optional embodiment, reaction tube 1 includes a reaction sleeve, and the reaction sleeve includes a reaction inner tube and a sleeve; the reaction inner tube and the sleeve are detachably connected; and the sleeve is used to connect to the reaction tank.

[0014] In an optional embodiment, the rotatable mechanism includes a protrusion; the protrusion is connected to the control device through a motor; a groove is provided on the outer side of the bottom of the inner reaction tube; and the groove is used to match and connect with the protrusion.

[0015] In an optional embodiment, the outer wall of the tube sleeve is provided with grooves / ridges; the grooves / ridges are arranged along the axial direction of the tube sleeve; the grooves / ridges on the outer wall of the tube sleeve are used to match and connect with the ridges / grooves on the inner side of the reaction tank wall.

[0016] In an optional embodiment, a stirring member is provided on the inner side of the tube wall of the inner reaction tube; the stirring member is provided at one end close to the bottom of the inner reaction tube.

[0017] In an optional embodiment, the number of the stirring members is at least two; the stirring members are evenly distributed on the inner side of the tube wall of the inner reaction tube.

[0018] The beneficial effects of the embodiments of the utility model are:

[0019] The antibody processing equipment provided by the utility model includes an enzyme cutting system and a purification system. The enzyme cutting system includes a heating mechanism, a reaction mechanism and a rotatable mechanism for connecting a reaction tube. The reaction mechanism includes a reaction tank. The heating mechanism is arranged at the periphery of the reaction tank, and the reaction temperature can be strictly controlled to ensure the uniformity and stability of each batch of products. The rotatable mechanism is used to connect with the reaction tube to ensure that the enzyme cutting reaction liquid or the purification reaction liquid fully reacts. The purification system includes a purification mechanism and a magnetic part. The purification mechanism includes a purification tank. The magnetic part is arranged at one end of the purification tank. The magnetic part is used to purify the substance to be purified in the purification tank, and the purification efficiency is high and the loss is small. The processing equipment is small in size, low in cost, and can efficiently and quickly obtain enzyme-cut Fab fragments, while reducing the differences between batches, so that the stability of the batches is guaranteed. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 The antibody processing device provided by the embodiment of the utility model;

[0022] Figure 2 A reaction tube 1 provided in an embodiment of the utility model;

[0023] Figure 3 This is the reaction tube 2 provided in the embodiment of the utility model.

[0024] Icons: 100-antibody processing equipment; 110-enzyme cleavage system; 111-reaction tank; 120-purification system; 121-purification tank; 130-power cord; 140-control device; 150-heating element; 151-tank wall; 152-outer side of heating element; 160-rotatable mechanism; 161-protrusion; 170-magnetic element; 200-reaction tube 1; 201-reaction tube 2; 210-reaction sleeve; 211-reaction inner tube; 212-tube sleeve; 213-stirring element; 214-groove element; 215-reaction inner tube cover; 220-enzyme cleavage tube; 221-enzyme cleavage tube cover; 222-magnetic particle buffer 1; 230-purification tube; 231-purification tube cover; 232-magnetic particle buffer 2. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0026] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0028] In the description of the present utility model, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, or are the directions or positional relationships in which the utility model product is usually placed when in use. They are only for the convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present utility model.

[0029] In addition, the terms "horizontal", "vertical" and the like do not mean that the components are required to be absolutely horizontal or suspended, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0030] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] Example

[0032] Please refer to Figure 1The present embodiment provides an antibody processing device 100, which includes an enzyme cleavage system 110 and a purification system 120; wherein the enzyme cleavage system 110 includes a heating mechanism (not marked in the figure), a reaction mechanism (not marked in the figure) and a rotatable mechanism 160 for connecting a reaction tube 200, the reaction mechanism includes a reaction tank 111, the heating mechanism includes a heating element 150, the rotatable mechanism 160 is arranged at the bottom of the inner side of the reaction tank 111, and the heating element 150 is arranged on the periphery of the reaction tank 111.

[0033] The reaction tank 111 is used to place the reaction tube 1 200, and the reaction tube 1 200 is used to place the reaction solution to be digested or purified, and the reaction tank 111 is used in combination with the reaction tube 1 200. In this embodiment, the reaction tank 111 is a columnar structure, specifically, a prism structure. In other embodiments of the utility model, the reaction tank 111 can also be set to a cylindrical structure or other structures.

[0034] Furthermore, evenly distributed ridges / grooves are provided on the inner side of the groove wall 151 of the reaction groove 111, and the ridges or grooves are evenly arranged along the axial direction of the reaction groove 111. In this embodiment, the inner wall of the reaction groove 111 is provided with ridges, and in other embodiments of the utility model, the inner side of the groove wall 151 of the reaction groove 111 can also be provided with grooves, or adjusted according to actual needs, and a structural member is provided.

[0035] In this embodiment, the bottom surface of the column structure of the reaction tank 111 is a plane, and a rotatable mechanism 160 is installed on the bottom surface of the reaction tank 111, and the rotatable mechanism 160 is used to connect with the reaction tube 1 200. In other embodiments of the utility model, the bottom surface of the reaction tank 111 can also be an arc bottom surface.

[0036] The protrusion 161 is a part of the rotatable mechanism 160, and the protrusion 161 is selected from any one of a straight protrusion and a cross-shaped protrusion. Further, the rotatable mechanism 160 also includes a connecting member (not shown in the figure), and the protrusion 161 and the connecting member are detachably connected, and the type of the protrusion 161 used can be reasonably selected and replaced according to actual needs. In this embodiment, the protrusion 161 is a cross-shaped protrusion 161. In other embodiments of the utility model, it can be replaced according to actual use needs and a straight protrusion 161 can be selected.

[0037] It should be noted that the protrusion 161 is provided to match and connect with the groove 214 provided at the bottom of the reaction inner tube 211, so as to fix the reaction inner tube 211 in the reaction tank 111, and prevent the reaction inner tube 211 from detaching from the reaction tank 111 and flying out when the motor drives the reaction inner tube 211 to rotate.

[0038] The connecting piece is used to connect the protruding piece 161 and the motor (not shown in the figure) and to connect to the control device 140. The control device 140 can control the switch and rotation of the motor, and further control the working condition and rotation condition of the protruding piece 161.

[0039] It should be noted that the motor can control the rotatable mechanism 160 to rotate forward or reverse. In this embodiment, the forward rotation and reverse rotation are alternately performed to ensure that the magnetic material in the tube is evenly distributed during the enzyme cleavage and purification processes, and the reaction is more complete. In other embodiments of the utility model, the rotation of the motor can also be selected only for forward rotation or only for reverse rotation.

[0040] Please refer to Figure 2 The present embodiment provides a reaction tube 200, wherein the reaction sleeve 210 comprises a reaction inner tube 211 and a tube sleeve 212, and a reaction inner tube cover 215 is provided on the top of the reaction inner tube 211. It should be noted that the reaction inner tube 211 and the tube sleeve 212 are installed in a detachable form.

[0041] Furthermore, the outer diameter of the tube sleeve 212 is set to match the radial width of the column of the reaction tank 111 , so that it can be placed exactly in the reaction tank 111 and fixedly connected to the reaction tank 111 .

[0042] Furthermore, evenly distributed grooves / ridges are provided on the outer wall of the tube sleeve 212 along the axial direction of the tube sleeve 212, which are used to match and connect with the ridges / grooves on the inner side of the groove wall 151 of the reaction groove 111. In this embodiment, the grooves are provided on the outer wall of the tube sleeve 212, which are connected with the ridges on the inner side of the groove wall 151 of the reaction groove 111.

[0043] Specifically, when placing the sleeve into the reaction tank 111 , it is necessary to match and snap-fit ​​the groove on the outer wall of the sleeve with the ridge on the inner side of the groove wall 151 of the reaction tank 111 , and then slide directly from the top to the bottom along the axial direction of the reaction tank 111 to connect the sleeve to the reaction tank 111 .

[0044] It should be noted that the existence of the tube sleeve 212 can prevent the inner reaction tube 211 from contacting the tank wall 151 of the reaction tank 111 during the rotation process, and reduce the probability of the inner reaction tube 211 detaching from the reaction tank 111 .

[0045] The inner reaction tube 211 can be rotated in the radial direction of the sleeve, but cannot be moved in the axial direction of the sleeve. When the reaction sleeve 210 is placed in the reaction tank 111, the sleeve 212 can be fixedly connected with the reaction tank 111, and the inner reaction tube 211 rotates in the radial direction of the reaction tank 111 under the drive of the motor. The existence of the sleeve 212 can prevent the inner reaction tube 211 from detaching from the reaction tank 111 and flying out during the rotation process.

[0046] The bottom outer side of the reaction inner tube 211 is provided with a groove member 214, which is selected from any one of a straight groove member and a cross groove member. The specification and size of the groove member 214 are the same as the protrusion member 161 at the bottom of the reaction tank 111, and the two are matched and can be detachably connected for use.

[0047] In this embodiment, the groove part 214 at the bottom of the reaction inner tube 211 is an integrally formed structure with the reaction inner tube 211, and can be directly prepared into a reaction inner tube 211 with a straight groove part 214 or a reaction inner tube 211 with a cross groove part 214, which can be selected according to actual needs.

[0048] Furthermore, in this embodiment, the groove member 214 is a cross-shaped groove member 214, which is fixedly engaged with the protrusion member 161. In other embodiments of the utility model, it can be replaced according to actual use needs, and a straight groove member 214 can be selected.

[0049] The matching connection between the groove member 214 and the protrusion member 161 further strengthens the connection between the reaction inner tube 211 and the reaction tank 111. In addition, the tube sleeve 212 arranged on the outside of the reaction inner tube 211 has a double fixing function, which can effectively prevent the reaction inner tube 211 from flying out of the reaction tank 111 during rotation.

[0050] It should be noted that in other embodiments of the present invention, whether to use the tube sleeve 212 can be selected according to the specifications of the reaction inner tube 211 actually used. If the diameter of the reaction inner tube 211 is not much different from the diameter of the reaction tank 111, the reaction inner tube 211 can be directly placed in the reaction tank 111 for reaction, and there is no need to install the tube sleeve 212 on the periphery of the reaction inner tube 211. Because the connection between the protrusion 161 at the bottom of the reaction tank 111 and the groove 214 at the bottom of the reaction inner tube 211 can fix the reaction inner tube 211 in the reaction tank 111, so that it will not break away and fly out of the reaction tank 111 during the rotation process. The existence of the tube sleeve 212 further strengthens the connection of the reaction inner tube 211.

[0051] A stirring member 213 is provided on the inner side of the tube wall of the reaction inner tube 211, and the stirring member 213 is fixedly connected to the tube wall of the reaction inner tube 211. In the present embodiment, the two are integrally formed. In other embodiments of the utility model, other connection methods between the stirring member 213 and the reaction inner tube 211 can be selected as needed, for example, a detachable connection method can be used to select whether to install the stirring member 213 as needed.

[0052] Further, in this embodiment, the stirring member 213 is arranged at one end of the bottom of the inner reaction tube 211, and the number of the stirring members 213 is set to at least two. In this embodiment, the number of the stirring members 213 is set to two, and the stirring members 213 are evenly distributed and symmetrically arranged on the inner side of the tube wall of the inner reaction tube 211. In other embodiments of the utility model, the number of the stirring members 213 is set according to actual needs.

[0053] It should be noted that the presence of the stirring member 213, when the reaction inner tube 211 is rotated, the stirring member 213 will rotate together therewith, and the reaction solution in the reaction inner tube 211 is stirred, so that the reaction of the reaction solution is more sufficient and the reaction is more thorough. In addition, in the present embodiment, the rotation of the reaction inner tube 211 is taken to be positive rotation and reverse rotation alternately, and the presence of the stirring member 213, so that the reaction efficiency of the reaction solution is improved, and the reaction uniformity is higher. In addition, the reaction is carried out under the condition of constant temperature, so that the controllability of the final enzyme reaction and the purification reaction is strengthened, which is conducive to improving the uniformity and stability of each batch of products.

[0054] In this embodiment, the heating element 150 is a constant temperature heating element 150, which is arranged at the periphery of the reaction tank 111, and the purpose is to provide a constant temperature for the reaction solution to be cleaved or purified, so as to avoid the influence of temperature changes on the reaction. During the heating process, strictly controlling the reaction temperature is conducive to improving the uniformity and stability of each batch of products.

[0055] Furthermore, the heating element 150 is annular in structure, extending from the top of the reaction tank 111 to the bottom of the reaction tank 111. A heating element (not shown) is arranged in the heating element 150, and the heating element is connected to the control device 140 to transfer heat to the heating element 150.

[0056] Specifically, a heat-conducting medium is placed between the heating element 150 and the reaction tank 111, and the groove wall 151 and the groove bottom of the reaction tank 111 are made of heat-conducting materials, which are convenient for rapid heat transfer to the inner reaction tube 211 and the reaction liquid in the tube. The outer side 152 of the heating element, that is, the side of the heating element 150 away from the reaction tank 111, is made of heat-insulating material. The heat-insulating material can effectively isolate heat transfer, so that heat is transferred to the direction of the reaction tank 111, reducing heat loss, improving heat transfer efficiency, and thus saving energy. In this embodiment, the heat-conducting medium is not limited, and the heat-conducting medium is a conventional heat-conducting material, such as water, silicone oil, etc.

[0057] In this embodiment, the heating device is also provided with a heat-conducting medium inlet (not marked in the figure) and a heat-conducting medium outlet (not marked in the figure), so as to facilitate regular replacement of the heat-conducting medium inside the heating element 150 and avoid excessive impurities causing a reduction in the heat transfer efficiency of the heating device due to long-term use, thereby causing increased energy loss.

[0058] The control device 140 is connected to the heating element of the heating member 150 and controls the switch of the heating element and sets the required temperature. In this embodiment, the control device 140 is arranged on the outer side 152 of the heating member, and the control device 140 is also connected to a power line 130 for communicating with a power source.

[0059] In this embodiment, the purification system 120 includes a purification mechanism and a magnetic component 170 . The purification mechanism includes a purification tank 121 . The magnetic component 170 is arranged at one end of the purification tank 121 . The main purpose of installing the magnetic component 170 is to purify the substance to be purified in the purification tank 121 .

[0060] Furthermore, the purification system 120 and the enzyme cutting system 110 are installed together, that is, the purification system 120 and the enzyme cutting system 110 are adjacent to and installed on the upper surface of the same equipment base (not marked in the figure). In other embodiments of the utility model, the purification system 120 and the enzyme cutting system 110 can be installed separately according to actual needs.

[0061] It should be noted that the setting of the equipment base can protect the equipment placed on it from direct contact with the ground, avoid spillage of reaction liquid during the reaction process and unclean cleaning, and avoid erosion of the bottom of the equipment by residual water on the ground.

[0062] In this embodiment, the magnetic member 170 is installed at one end of the purification mechanism away from the heating member 150, and the purification tank 121 is arranged at one end of the purification mechanism close to the magnetic member 170. In other embodiments of the utility model, the specific installation position of the magnetic member 170 can be reasonably adjusted according to actual needs.

[0063] Further, in this embodiment, the magnetic member 170 is extended along the top of the purification mechanism to the bottom of the purification mechanism on one side of the purification mechanism, that is, the vertical height of the magnetic member 170 is greater than the vertical height of the purification tank 121. In other embodiments of the utility model, the size of the magnetic member 170 can be adjusted according to actual needs.

[0064] It should be noted that the magnetic element 170 is arranged on one side of the purification mechanism. Its purpose is to adsorb the magnetic substance in the reaction tube 201 to one side of the inner wall of the tube, so that it can be precipitated as close to the inner wall of the tube as possible, so as to facilitate the subsequent pipetting operation to transfer the purified liquid from the side of the tube away from the magnetic element 170.

[0065] In this embodiment, the purification tank 121 is a columnar structure with an arc-shaped bottom. In other embodiments of the present invention, the bottom of the purification tank 121 can also be set to a plane.

[0066] Please refer to Figure 3, the reaction tube 201 includes an enzyme cutting tube 220 and a purification tube 230. In this embodiment, the enzyme cutting tube 220 is a cylindrical tube with an arc-shaped bottom surface. A sealing film (not shown in the figure) and an enzyme cutting tube cover 221 are provided on the top of the enzyme cutting tube 220. A magnetic particle buffer 1 222 is placed in the enzyme cutting tube 220. The magnetic particle buffer 1 222 includes 0.03% P300, 0.1M PBS, and an endonuclease is coupled to the magnetic particles. The endonuclease is selected from any one of pepsin, papain or IdeS degradation enzyme. The pH of the magnetic particle buffer 1 222 is 7.4. The magnetic particles in the magnetic particle buffer 1 222 can be adsorbed by the magnetic member 170 and gathered on one side of the tube wall.

[0067] In this embodiment, the purification tube 230 is also a column with an arc-shaped bottom surface. A sealing film (not shown in the figure) and a purification tube cover 231 are provided on the top of the purification tube 230. A magnetic particle buffer 2232 is placed in the purification tube 230. The magnetic particle buffer 2232 includes 0.03% P300, 0.1M PBS, and affinity purification proteins are coupled to the magnetic particles. The affinity purification proteins are selected from either protein A or protein G. The pH of the magnetic particle buffer 2232 is 7.4. The magnetic particles in the magnetic particle buffer 2232 can be adsorbed by the magnetic member 170 and gathered on one side of the tube wall.

[0068] It should be noted that the specifications of the enzyme cutting tube 220 and the purification tube 230 in this embodiment are consistent, and the main difference is that the buffer components in the tubes are different. Among them, the magnetic particle buffer 1 222 contained in the enzyme cutting tube 220 and the magnetic particle buffer 2 232 contained in the purification tube 230 are homemade buffers. The prepared buffers are loaded into the corresponding reaction tubes and sealed, and then sealed and stored for standby use. In other embodiments of the utility model, the magnetic particle buffer can also be purchased.

[0069] Please refer to Figure 1 and Figure 2 and Figure 3 The antibody processing device 100 provided by the present invention has the following working principle:

[0070] S1. Open the enzyme cutting tube cover 221, tear off the sealing film on the top of the tube mouth of the enzyme cutting tube 220, add a certain proportion of full-length antibody, cover the enzyme cutting tube cover 221 to mix the magnetic particle buffer 1222 and the full-length antibody in the enzyme cutting tube 220, and after mixing, transfer the mixed solution to the reaction inner tube 211 of the reaction sleeve 210.

[0071] It should be noted that there is no limitation on the mixing method in this operation, and the distribution state of the magnetic particles inside the tube can be observed through the tube wall to preliminarily determine whether the mixing is uniform.

[0072] S2. Place the reaction sleeve 210 in S1 into the reaction tank 111, connect the power cord 130, adjust the temperature on the control device 140 to 37°C, and obtain the suspension 1 after reacting for 2 hours. Pour the suspension 1 back into the enzyme cutting tube 220, and then clean the reaction inner tube 211.

[0073] It should be noted that during the 2h reaction, the rotation of the suspension is carried out in the form of alternating forward rotation and reverse rotation. In this embodiment, the reaction time is not limited and can be reasonably adjusted according to actual needs.

[0074] S3. Place the enzyme cutting tube 220 containing the suspension 1 in S2 into the purification tank 121 and let it stand for a period of time, then transfer it to the purification tube 230.

[0075] The specific operation is as follows: open the purification tube cover 231 and tear off the sealing film on the top of the purification tube 230. Use a pipette to slowly insert the end of the enzyme cutting tube 220 away from the magnetic member 170 into the enzyme cutting tube 220 for pipetting and transfer the liquid to the purification tube 230.

[0076] It should be noted that during the pipetting process, the enzyme cutting tube 220 is always placed in the purification tank 121 and remains stationary, and the pipette does not touch the magnetic particles that have been adsorbed to the inner wall of the tube.

[0077] S4. Cover the purification tube 230 containing the suspension 1 in S3 with the purification tube cover 231 and mix them. After mixing, transfer the mixed liquid to the reaction inner tube 211 of the reaction sleeve 210.

[0078] The method for judging whether the mixing is uniform is to observe the distribution state of the magnetic particles inside the tube wall to preliminarily judge whether the mixing is uniform.

[0079] S5. Place the reaction sleeve 210 in S4 into the reaction tank 111, turn on the power, adjust the temperature to 37° C., react for 30 minutes to obtain the suspension 2, and pour the suspension 2 back into the purification tube 230.

[0080] S6. Place the purification tube 230 in S5 into the purification tank 121 and let it stand for a period of time to obtain a purified liquid; suck out the liquid after the reaction, which is the final Fab antibody liquid after enzyme cleavage and purification, and store it for later use.

[0081] For the procedure of aspirating the liquid after the reaction, refer to the pipetting operation in S3.

[0082] In summary, the antibody processing equipment 100 provided by the utility model is small in size and low in cost, and can realize one device to complete enzyme cleavage and purification at the same time, which is simple and efficient; the enzyme cleavage tube 220 pre-filled with enzyme cleavage reaction solution and the purification tube 230 pre-filled with purification reaction solution are sealed and stored, and the reaction temperature is strictly controlled during the reaction, which can improve the uniformity and stability of each batch of products; the use of magnetic particles to separate the endonuclease completed by enzyme cleavage is more efficient than traditional dialysis and gel filtration, and the separation is more complete; in addition, the use of magnetic particles to purify and separate reactants is more efficient, has less loss, and has smaller batch differences than traditional chromatographic separation columns.

[0083] The above description is only the preferred embodiment of the utility model, and is not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. An antibody processing device, It is characterized in that The processing equipment includes an enzyme cutting system and a purification system; The enzyme cutting system comprises a heating mechanism, a reaction mechanism and a rotatable mechanism for connecting a reaction tube, the reaction mechanism comprises a reaction tank, the heating mechanism is arranged at the periphery of the reaction tank, and the rotatable mechanism is arranged at the bottom of the reaction tank; The purification system comprises a purification mechanism and a magnetic component. The purification mechanism comprises a purification tank. The magnetic component is arranged at one end of the purification tank and is used to purify the substance to be purified in the purification tank.

2. The processing device according to claim 1, It is characterized in that The purification system also includes a reaction tube 2; The second reaction tube is used to hold the substance to be purified; The reaction tube 2 is used in conjunction with the purification tank.

3. The processing device according to claim 1, It is characterized in that The processing device also includes a control device; The control device is connected to the heating element of the heating mechanism and controls the switch and temperature of the heating element; The control device is also connected to the rotatable mechanism and is used to control the switch and rotation of the rotatable mechanism.

4. The processing device according to claim 1, It is characterized in that The heating mechanism comprises a heating element, which is in an annular structure and extends from the top to the bottom of the reaction tank; A heat-conducting medium is placed between the heating element and the reaction tank, and the outer side of the heating element is made of a heat-insulating material.

5. The processing device according to claim 4, It is characterized in that The wall and bottom of the reaction tank are made of heat-conducting materials; The inner side of the groove wall of the reaction groove is provided with convex ridges / grooves; The ridges / grooves are arranged along the axial direction of the reaction tank.

6. The processing device according to claim 1, It is characterized in that The reaction tube 1 comprises a reaction sleeve, and the reaction sleeve comprises a reaction inner tube and a sleeve; the reaction inner tube and the sleeve are detachably connected; and the sleeve is used to be connected to the reaction tank.

7. The processing device according to claim 6, It is characterized in that The outer wall of the pipe sleeve is provided with grooves / ridges; The grooves / ridges are arranged along the axial direction of the sleeve; The grooves / ridges on the outer wall of the tube sleeve are used to match and connect with the ridges / grooves on the inner side of the groove wall of the reaction groove.

8. The processing device according to claim 6, It is characterized in that A stirring member is arranged on the inner side of the tube wall of the inner reaction tube; The stirring member is arranged at one end close to the bottom of the inner reaction tube.

9. The processing device according to claim 8, It is characterized in that The number of the stirring members is at least two; The stirring members are evenly distributed on the inner side of the tube wall of the inner reaction tube.

10. The processing device according to claim 1, It is characterized in that The rotatable mechanism comprises a protruding member; the protruding member is connected to the control device via a motor; A groove piece is arranged on the outer side of the bottom of the inner reaction tube; the groove piece is used for matching and connecting with the protrusion piece.

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