Multifunctional cell culture tank

Abstract

The utility model relates to the technical field of cell culture tanks, in particular to a multifunctional cell culture tank which comprises a tank body, a constant-temperature sleeve is fixedly sleeved on the outer side of the tank body, a liquid storage space is arranged between the tank body and the constant-temperature sleeve, the upper side of the tank body is fixedly connected with a driving motor, and hot water with proper temperature is injected into the liquid storage space. The driving motor drives the hollow stirring shaft to rotate, and the hollow stirring shaft drives the stirring blades to stir a culture solution, so that the culture solution is uniformly heated, a comfortable culture temperature can be provided for cells, and meanwhile, filtered and disinfected oxygen enters the hollow stirring shaft from the air supply pipe and then is aerated into the tank body from the rotating aeration pipe; the oxygen supply pipe is arranged in the liquid storage space, the stirring effect is matched, oxygen needed by cell growth can be fully supplemented, the oxygen supply pipe is spirally distributed in the liquid storage space, the oxygen can be effectively heated, the situation that the temperature difference between the oxygen and a culture solution is too large is avoided, and the trouble of independently heating the oxygen is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture tanks, in particular to a multifunctional cell culture tank. Background Art

[0002] Cell growth requires a nutritional environment. The nutrient matrix used to maintain cell growth is called a culture medium. Culture media can be divided into liquid culture media and solid culture media according to their physical state. Liquid culture media are used in large-scale industrial production and basic theoretical research such as physiological metabolism. During the culture process, the liquid culture medium is placed in a cell culture tank, which provides the required growth environment for the cells.

[0003] Chinese patent publication number CN208414449U discloses a stirred cell culture tank, in which an electric heating plate heats the gas to be transported to the stirred tank so that the gas reaches the same temperature as the culture liquid in the stirred tank, thereby avoiding the adverse effects of temperature differences on the cells cultured in the stirred tank. However, the above technology heats the cell culture gas alone, causing unnecessary trouble, and the fixed structure of the diffuser plate makes it difficult to fully aerate the culture tank, which results in a limited contact area between the gas and the culture liquid in the tank body, limiting the oxygen replenishment effect. Therefore, the present utility model provides a multifunctional cell culture tank to solve the above problems. Utility Model Content

[0004] The purpose of the utility model is to provide a multifunctional cell culture tank, which solves the technical problem that traditional cell culture tanks heat the cell culture gas separately, causing unnecessary trouble, and the fixed structure of the diffuser plate makes it difficult to fully aerate the culture tank, which leads to a limited contact area between the gas and the culture liquid in the tank body, limiting the oxygen supplement effect.

[0005] The above technical objectives of the present invention are achieved through the following technical solutions:

[0006] A multifunctional cell culture tank comprises a tank body, a thermostatic sleeve is fixedly provided on the outside of the tank body, a liquid storage space is formed between the tank body and the thermostatic sleeve, a driving motor is fixedly connected to the upper side of the tank body, and a hollow stirring shaft is fixedly connected to the output end of the driving motor, wherein a plurality of aeration tubes are fixedly embedded on both sides of the hollow stirring shaft, aeration holes are densely distributed on the lower sides of the aeration tubes, stirring blades are fixed between the aeration tubes and the hollow stirring shaft, and an air supply pipe is fixedly embedded on one side of the thermostatic sleeve, the part of the air supply pipe located in the liquid storage space has a spiral structure, one end of the air supply pipe is fixedly connected to a sealing bin after passing through the tank body, and the bottom end of the hollow stirring shaft is rotatably embedded in the top wall of the sealing bin.

[0007] Beneficial effects: This multifunctional cell culture tank injects hot water of suitable temperature into the liquid storage space to heat the tank body in a water bath. The driving motor drives the hollow stirring shaft to rotate, and the hollow stirring shaft drives the stirring blades to stir the culture liquid, so that the culture liquid is heated evenly, which can provide cells with a comfortable culture temperature. At the same time, filtered and sterilized oxygen enters the hollow stirring shaft from the air supply pipe, and is then aerated into the tank body from the rotating aeration pipe. Combined with the stirring effect, it can fully supplement the oxygen required for cell growth. The air supply pipe is spirally distributed in the liquid storage space, so that the oxygen can be effectively heated without causing a large temperature difference between the oxygen and the culture liquid, avoiding the trouble of heating the oxygen separately.

[0008] Furthermore, the aeration pipe is provided with multiple layers along the hollow stirring shaft, and the number of aeration holes on the aeration pipe increases layer by layer from top to bottom.

[0009] Furthermore, a liquid inlet pipe is fixedly embedded in the upper part of the thermostatic sleeve, and a liquid discharge pipe is fixedly embedded in the bottom of the thermostatic sleeve. The liquid inlet pipe and the liquid discharge pipe are respectively located on both sides of the thermostatic sleeve.

[0010] Furthermore, a feeding port and an exhaust pipe are fixedly embedded on the upper side of the tank body, and the feeding port is threadedly connected to the tank cover.

[0011] Furthermore, a discharge pipe and a bracket are fixedly connected to the lower side of the tank body, and a manual valve is installed on the discharge pipe.

[0012] Furthermore, three oblique support rods are fixedly connected to the lower side of the sealed chamber, and the bottom ends of the oblique support rods are fixedly connected to the bottom wall of the tank body. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of the three-dimensional structure of a multifunctional cell culture tank of the present utility model;

[0014] Figure 2 This is a side structural cross-sectional view of a multifunctional cell culture tank of the present utility model;

[0015] Figure 3 This is a side sectional view of the connection structure between the hollow stirring shaft and the sealing chamber in a multifunctional cell culture tank of the present invention;

[0016] Figure 4 This is a top cross-sectional view of the connection structure between the hollow stirring shaft and the sealing chamber in a multifunctional cell culture tank of the present invention.

[0017] Figure numerals: 1. Tank body; 11. Feeding port; 12. Exhaust pipe; 13. Tank cover; 14. Discharge pipe; 15. Bracket; 16. Manual valve; 2. Constant temperature jacket; 21. Liquid inlet pipe; 22. Liquid discharge pipe; 3. Drive motor; 4. Hollow stirring shaft; 41. Aeration pipe; 42. Aeration hole; 43. Stirring blade; 5. Air supply pipe; 6. Sealing chamber; 61. Diagonal support rod. DETAILED DESCRIPTION

[0018] The following description is merely a preferred embodiment of the present invention, and the scope of protection is not limited to this embodiment. All technical solutions based on the principles of the present invention should be included in the scope of protection of the present invention. It should also be noted that improvements and modifications that do not depart from the principles of the present invention by ordinary skill in the art should also be considered within the scope of protection of the present invention.

[0019] See Figures 1 to 4 As shown, a multifunctional cell culture tank comprises a tank body 1, wherein a feeding port 11 and an exhaust pipe 12 are fixedly embedded on the upper side of the tank body 1, and a tank cover 13 is threadedly connected to the feeding port 11;

[0020] Specifically, by unscrewing the tank cover 13, nutrients required for cell growth can be added from the feeding port 11, and the exhaust pipe 12 can discharge waste gas generated by cell growth to prevent waste gas accumulation from changing the culture environment.

[0021] Furthermore, a discharge pipe 14 and a bracket 15 are fixedly connected to the lower side of the tank body 1, and a manual valve 16 is installed on the discharge pipe 14;

[0022] Specifically, the manual valve 16 can be opened to discharge the culture medium, which can facilitate sampling or unloading.

[0023] Furthermore, a thermostatic sleeve 2 is fixedly provided on the outside of the tank body 1, and a liquid storage space is provided between the tank body 1 and the thermostatic sleeve 2. A liquid inlet pipe 21 is fixedly embedded in the upper part of the thermostatic sleeve 2, and a liquid discharge pipe 22 is fixedly embedded in the bottom of the thermostatic sleeve 2. The liquid inlet pipe 21 and the liquid discharge pipe 22 are respectively located on both sides of the thermostatic sleeve 2.

[0024] Specifically, hot water of suitable temperature enters the liquid storage space through the liquid inlet pipe 21 and is then discharged through the liquid discharge pipe 22, which can maintain the hot water in the liquid storage space at a constant temperature. The hot water can heat the tank body 1 in a water bath, so that the culture fluid is heated evenly, providing the cells with a comfortable culture temperature.

[0025] Furthermore, a drive motor 3 is fixedly connected to the upper side of the tank body 1, and a hollow stirring shaft 4 is fixedly connected to the output end of the drive motor 3, wherein a plurality of aeration tubes 41 are fixedly embedded on both sides of the hollow stirring shaft 4, and aeration holes 42 are densely distributed on the lower side of the aeration tubes 41, and stirring blades 43 are fixed between the aeration tubes 41 and the hollow stirring shaft 4;

[0026] Specifically, the driving motor 3 drives the hollow stirring shaft 4 to rotate, and the hollow stirring shaft 4 drives the stirring blades 43 to rotate. The stirring blades 43 stir the culture solution so that the culture solution is fully mixed.

[0027] Furthermore, the aeration pipe 41 is provided with multiple layers along the hollow stirring shaft 4, and the number of aeration holes 42 on the aeration pipe 41 increases layer by layer from top to bottom;

[0028] Specifically, this distribution method can fully and evenly supply oxygen to all parts of the tank body 1, and combined with the stirring effect, can fully replenish the oxygen required for cell growth.

[0029] Furthermore, an air supply pipe 5 is fixedly embedded on one side of the thermostatic sleeve 2, and the portion of the air supply pipe 5 located in the liquid storage space is in a spiral structure;

[0030] Specifically, the oxygen can be effectively heated, and the temperature after heating is consistent with the temperature of the culture solution, which will not cause a large temperature difference between the oxygen and the culture solution, avoiding the trouble of heating the oxygen separately.

[0031] Furthermore, one end of the air supply pipe 5 passes through the tank body 1 and is fixedly connected to the sealed chamber 6. The bottom end of the hollow stirring shaft 4 is rotatably embedded in the top wall of the sealed chamber 6. The lower side of the sealed chamber 6 is fixedly connected to three diagonal support rods 61. The bottom ends of the diagonal support rods 61 are all fixedly connected to the bottom wall of the tank body 1.

[0032] Specifically, the diagonal support rods 61 can provide sufficient support for the sealed chamber 6 without affecting the discharge of the liquid culture medium in the cell culture tank by the discharge pipe 14 .

[0033] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A multifunctional cell culture tank, comprising a tank body (1), characterized in that: A thermostatic sleeve (2) is fixedly provided on the outer side of the tank body (1), a liquid storage space is formed between the tank body (1) and the thermostatic sleeve (2), a driving motor (3) is fixedly connected to the upper side of the tank body (1), and a hollow stirring shaft (4) is fixedly connected to the output end of the driving motor (3), wherein a plurality of aeration pipes (41) are fixedly embedded on both sides of the hollow stirring shaft (4), aeration holes (42) are densely distributed on the lower sides of the aeration pipes (41), stirring blades (43) are fixedly provided between the aeration pipes (41) and the hollow stirring shaft (4), and an air supply pipe (5) is fixedly embedded on one side of the thermostatic sleeve (2), the portion of the air supply pipe (5) located in the liquid storage space is in a spiral structure, one end of the air supply pipe (5) passes through the tank body (1) and is fixedly connected to a sealing chamber (6), and the bottom end of the hollow stirring shaft (4) is rotatably embedded in the top wall of the sealing chamber (6).

2. A multifunctional cell culture tank according to claim 1, characterized in that: The aeration pipe (41) is provided with multiple layers along the hollow stirring shaft (4), and the number of aeration holes (42) on the aeration pipe (41) increases layer by layer from top to bottom.

3. The multifunctional cell culture tank according to claim 1, characterized in that: A liquid inlet pipe (21) is fixedly embedded in the upper part of the thermostatic jacket (2), and a liquid discharge pipe (22) is fixedly embedded in the bottom of the thermostatic jacket (2). The liquid inlet pipe (21) and the liquid discharge pipe (22) are respectively located on both sides of the thermostatic jacket (2).

4. The multifunctional cell culture tank according to claim 1, characterized in that: A feeding port (11) and an exhaust pipe (12) are fixedly embedded on the upper side of the tank body (1), and a tank cover (13) is threadedly connected to the feeding port (11).

5. The multifunctional cell culture tank according to claim 1, characterized in that: A discharge pipe (14) and a bracket (15) are fixedly connected to the lower side of the tank body (1), and a manual valve (16) is installed on the discharge pipe (14).

6. The multifunctional cell culture tank according to claim 1, characterized in that: Three oblique support rods (61) are fixedly connected to the lower side of the sealing chamber (6), and the bottom ends of the oblique support rods (61) are fixedly connected to the bottom wall of the tank body (1).

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