Drum-type protein desalting device

Abstract

The utility model relates to the technical field of desalting and filtering devices, in particular to a drum-type protein desalting device which comprises a box body, a drum with a horizontal central axis is arranged in the box body, the drum consists of a drum body and a drum cover, the drum body comprises an inner drum and an outer drum, the drum bottoms of the inner drum and the outer drum are fixed, and the top openings of the inner drum and the outer drum are in the same direction and are flush with each other; a gap exists between the inner cylinder and the outer cylinder, and filler is placed in the gap. Filtering holes are uniformly distributed in the surfaces of the inner cylinder and the outer cylinder; the opening side of the cylinder body is connected with a cylinder cover, and a gear is arranged on the cylinder cover. According to the utility model, the filler is arranged between the two drums of the roller, and the filter holes are formed in the two drums, so that on one hand, the filler is arranged between the two drums to play a role in limiting and protecting, and the deformation and random accumulation of the filler are avoided; and on the other hand, the gear transmits power to be matched with the roller to rotate, the whole inner wall of the inner cylinder is converted into a filtering surface, and the rotation is ingeniously applied, so that the filtering contact area is greatly increased, the filtering efficiency is improved, and the protein desalting effect is further improved.

Description

Technical Field

[0001] The utility model relates to the technical field of desalination and filtering devices, in particular to a drum-type protein desalination device. Background Art

[0002] Proteins are vital components of organisms. They play a key role in the structure and function of cells. Therefore, protein research is an important research direction in many fields such as biology, biochemistry, and molecular biology. At the same time, when studying proteins, the purity of proteins directly affects the authenticity and accuracy of experimental results, so it is necessary to purify proteins before research. In the process of protein extraction and purification, such as dissolution, precipitation, filtration, etc., salt substances are often used as buffers to adjust the pH value, ionic strength or conductivity of the solution, thereby facilitating the dissolution, stability and separation of proteins; in addition, salt substances can also help reduce the nonspecific interaction of proteins with other non-target substances and improve the efficiency of purification. However, too much salt substances may affect the properties and applications of proteins. For example, high salt concentrations may lead to decreased protein stability, loss of enzyme activity, or changes in interactions with other molecules. Therefore, in the preparation and purification process, it is usually necessary to strictly control the type and concentration of salts, and try to remove these salt substances in the final solution to obtain higher purity proteins.

[0003] In the patent with the authorization announcement number CN209161906U, a protein purification device is provided, which reduces the internal pressure by manually pulling the vacuum component to promote the filtration of the solution through the enrichment component provided with a filler. The filler retains the protein in the solution and filters the non-protein substances such as salt to achieve the purpose of desalination and purification, wherein the filler is placed at the bottom of the enrichment component, and only the upper surface of the filler is in contact with the solution. When a large amount of solution needs to be filtered, the filtration speed is affected due to the limited contact area between the solution and the filler, resulting in low filtration efficiency. At the same time, the filler is placed in the enrichment component. For the method of having no means of protection for the filler and no restrictions on the movement of the filler, when there is a large amount of solution to be filtered, the filler may be deformed and accumulated due to the impact of the large amount of solution, resulting in reduced filtration efficiency.

[0004] In the patent with authorization announcement number CN211311348U, a protein purification device is provided, which filters the salts in the protein solution through a semipermeable membrane, and then uses an isolation sieve plate with a specific pore size to filter other non-protein substances and retain the protein to achieve the effect of desalting and purification. Similarly, in the process of filtering salts, the contact area between the semipermeable membrane and the solution is limited, which affects the filtration efficiency. During the subsequent isolation sieve plate filtration, there is also a problem of low filtration efficiency due to the limited contact area. Utility Model Content

[0005] The utility model aims to provide a drum type protein desalting device in view of the deficiencies of the prior art.

[0006] To achieve this purpose, the solution adopted by the utility model is: a drum-type protein desalting device, including a box body, a drum with a horizontal central axis is arranged in the box body, the drum is composed of a cylinder body and a cylinder cover, the cylinder body includes an inner cylinder and an outer cylinder, the cylinder bottoms of the inner cylinder and the outer cylinder are fixed, and the top openings are in the same direction and flush; there is a gap between the inner cylinder and the outer cylinder, and a filler is placed in the gap; filtering holes are evenly distributed on the surfaces of the inner cylinder and the outer cylinder; one side of the cylinder body opening is connected to the cylinder cover.

[0007] Preferably, the cross-section of the gap in the vertical direction is annular, and a plurality of isolation blocks are nested and inserted into the gap at equal intervals in the horizontal direction. The isolation blocks divide the gap into a plurality of placement chambers, and the fillers are placed in the placement chambers.

[0008] Preferably, the cylinder cover comprises a cylinder cover body, and two layers of cylindrical protrusions and gears are provided at the center of one side of the cylinder cover body, and a cylindrical block is also provided on the same side surface.

[0009] Preferably, a protrusion is provided on the outside of one side of the opening of the barrel, and the protrusion is movably connected to a fastener, and the fastener can be rotationally adjusted based on the protrusion; the fastener and the cylindrical block can be snap-fitted and connected.

[0010] Preferably, a waste liquid collection box, a motor and a gear assembly are also provided inside the box body. The waste liquid collection box is placed at the bottom of the box body, and the drum is placed directly above the waste liquid collection box. In the horizontal direction, the cross-sectional area of ​​the waste liquid collection box is larger than the cross-sectional area of ​​the drum.

[0011] Preferably, the output end of the motor is connected to one end of the gear assembly and meshes with the transmission gear in the gear assembly; the other end of the gear assembly meshes with the gear through the transmission gear, and the gear forms a whole through the drum cover and the drum.

[0012] Beneficial effects achieved by the utility model:

[0013] 1. In the utility model, a drum with a horizontal central axis is provided, a filler is placed between the two drums, and filter holes are provided on the two drums. On the one hand, placing the filler between the two drums plays a role of limitation and protection, thereby avoiding deformation and random accumulation of the filler; on the other hand, the inner wall of the entire inner drum is converted into a filter surface by transmitting power through gears and coordinating the rotation of the drum. Compared with the prior art, the filtration efficiency is limited by the contact area. The utility model cleverly uses rotation to greatly increase the filtration contact area, improve the filtration efficiency, and thus improve the protein desalination effect.

[0014] 2. In the utility model, an isolation block is set between the two drums of the drum. The isolation block separates the filler that was originally connected into multiple parts, avoiding the problem of gaps between the fillers being stacked and accumulated due to rotation and gravity during the rotation of the drum, resulting in the problem of filler vacancies in local areas. By setting the isolation block, the problems that may occur during the filtration process are fully taken into consideration, and the filtration efficiency is also improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a three-dimensional structural schematic diagram of the utility model drum type protein desalination device.

[0016] Figure 2 The utility model is a three-dimensional structural schematic diagram of a drum of a drum-type protein desalting device.

[0017] Figure 3 It is a three-dimensional structural schematic diagram of the drum body of the utility model drum type protein desalination device.

[0018] Figure 4 The utility model is a three-dimensional structural schematic diagram of a drum cover of a drum-type protein desalting device.

[0019] Figure 5 The utility model is a three-dimensional structural schematic diagram of a liquid injection component of a drum-type protein desalination device.

[0020] Figure 6 It is a three-dimensional structural schematic diagram of a housing of a drum-type protein desalting device of the utility model.

[0021] Explanation of the reference numerals in the accompanying drawings: 1. roller, 11. cylinder body, 111. protrusion, 112. fastener, 113. inner cylinder, 114. outer cylinder, 115. isolation block, 12. cylinder cover, 121. cylindrical block, 122. gear, 123. cylinder cover body, 124. external thread, 125. annular gasket; 2. liquid injection assembly, 21. liquid injection pipe, 22. valve, 23. gasket, 24. rolling bearing; 31. box body, 311. gear assembly, 312. partition, 313. limiting block, 314. transmission gear; 32. front window; 4. waste liquid collection box; 5. control assembly; 6. motor; 7. vacuum device. DETAILED DESCRIPTION

[0022] The technical solution described in the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the present invention.

[0023] like Figure 1The drum-type protein desalting device comprises a drum 1, a liquid injection component 2, a box body 31, a front window 32 and a waste liquid collecting box 4. The front side of the box body 31 is connected to the front window 32, and the drum 1 with a horizontal central axis, the liquid injection component 2 and the waste liquid collecting box 4 are placed inside. The drum 1 is placed above the waste liquid collecting box 4. The drum 1 is used to filter the protein solution, and the waste liquid collecting box 4 is used to collect the filtered non-protein solution. At the same time, in the horizontal direction, the cross-sectional area of ​​the waste liquid collecting box 4 is larger than the cross-sectional area of ​​the drum 1, which ensures that when the non-protein solution in the drum 1 flows out, it can enter the waste liquid collecting box 4, thereby avoiding leakage of the solution and insufficient collection protection.

[0024] Furthermore, the front window 32 is composed of a frame, glass, and a rubber gasket. The glass is embedded in the frame, and the internal situation of the device can be observed through the glass. At the same time, the rubber gasket surrounds one side of the frame, so that when the front window 32 and the box body 31 are buckled and combined, it plays a sealing role. The front window 32 is rotatably connected to the bottom of the box body 31.

[0025] It should be noted that in the filtering process mentioned in the present invention, the working environment must be sealed, that is, the interior of the device must be in a sealed state.

[0026] like Figure 1-4 The drum 1 of the utility model is composed of a cylinder body 11 and a cylinder cover 12. The cylinder body 11 is composed of two cylinders with different diameters, namely an inner cylinder 113 and an outer cylinder 114. The bottoms of the two cylinders are fixed, and the openings of the two cylinders are in the same direction and flush; there is a gap between the inner cylinder 113 and the outer cylinder 114, and the cross-section of the gap in the vertical direction is annular. The isolation block 115 is horizontally nested and inserted into the gap in a circumferentially equidistant manner, and then the gap is equally divided by the isolation block 115 into a plurality of placement chambers for placing fillers, and the fillers can be C18 bonded silica gel fillers; filter holes are evenly distributed on the inner cylinder 113 and the outer cylinder 114, and the filter holes on the inner cylinder 113 correspond concentrically to the filter holes on the outer cylinder 114. When the protein solution is placed inside the drum 1, it can contact the filler through the filter holes of the inner drum 113 to adsorb the protein, and then filter the salt solution through the filter holes of the outer drum 114. At the same time, the drum 1 is rotated in conjunction with the gear 122 to allow the protein solution to fully contact the inner wall of the inner drum 113 of the drum, converting the entire inner wall of the inner drum 113 into a filtering surface, greatly increasing the contact area between the protein solution and the filler, improving the adsorption effect of the filler on the protein, and further improving the filtration efficiency of protein desalination.

[0027] Furthermore, one side of the opening of the cylinder body 11 is connected to the cylinder cover 12, and the other side is connected to the injection assembly 2. A protrusion 111 is provided on the outside of the opening side of the cylinder body 11, and the protrusion 111 is movably connected to a fastener 112; it needs to be explained in detail that the length of the isolation plate 115 starts from the closed end of the cylinder body 11 and extends to the open end, but a margin is left from the open end. The inner wall of the outer cylinder 114 and the outer wall of the inner cylinder 113 of the margin are both provided with threaded grooves. The role of the isolation block 115 is to isolate the filler that was originally connected as a whole into multiple parts, so as to avoid the gaps between the fillers being superimposed and accumulated due to rotation and gravity during the rotation of the drum, resulting in the problem of filler vacancies in local areas, affecting protein adsorption, and then affecting the filtration work of protein liquid desalination; after isolation by the isolation plate 115, the influence of the stacking of the filler gaps is weakened, thereby ensuring the normal filtration work.

[0028] Furthermore, the barrel cover 12 includes a barrel cover body 123, and two layers of cylindrical protrusions and a gear 122 are provided in the center of one side of the barrel cover body 123, and a cylindrical block 121 is also provided on the same side surface. The cylindrical block 123 can cooperate with the fastener 112 to play a fixing role; the other side of the barrel cover body 123 is provided with external threads 124 and circular washers 125 from the inside to the outside, and the external threads 124 can engage with the above-mentioned thread groove to form a threaded connection; the circular washer 125 is made of rubber.

[0029] Furthermore, when the cylinder cover 12 and the cylinder body 11 need to be buckled together, the external thread 124 on the cylinder cover 12 and the thread groove on the cylinder body 11 are engaged through a threaded connection. At the same time, there is a circular gasket 125 made of rubber on the cylinder cover 12. During the threaded connection and engagement process, the circular gasket 125 is squeezed and compressed to play a sealing role. When the cylinder cover 12 is tightened, the fastener 112 is buckled with the cylindrical block 121 to strengthen the airtightness and stability of the cylinder.

[0030] like Figure 1 , 2 5. The liquid injection component 2 of the utility model includes a liquid injection tube 21, on which a valve 22 is arranged. The rotating handle of the valve 22 extends to the outside of the box body 31. The valve 22 is a gate valve. The characteristic of the gate valve is that it has only two states: a fully closed state and a fully open state. By virtue of the characteristics of the gate valve, the problem of protein liquid being injected into the liquid injection tube and air entering the liquid injection tube during the filtration process due to improper valve closure can be effectively avoided; a rubber gasket 23 is connected to one end of the liquid injection tube 21, and a straight tube is arranged at the other end of the gasket 23, and the straight tube is connected to the circular opening of the cylinder body 11; a rolling bearing 24 is also arranged in the gasket 23, and the rolling bearing 24 here cooperates with the rotation of the drum 1 to play a role of auxiliary rotation and support the drum 1.

[0031] like Figure 1 , 56, the box 31 of the utility model is provided with a partition 312, a motor 6 and an air extraction device 7 inside, and the partition 312 is connected to the gasket 23 in the liquid injection component 2, and is used to support the liquid injection component 2; the outer side of the box 31 is also provided with a control component 5, which is electrically connected to the motor 6 and the air extraction device 7, respectively, and is used to control the start and shut down of the motor 6 and the air extraction device 7. The air extraction device 7 is placed at the bottom of the box 31, and is responsible for extracting the air inside the box 31, so that a negative pressure space is formed inside the box 31, and the filtering process is promoted by the negative pressure; specifically, when working, the air in the box 31 is evacuated by the air extraction device 7 to form a negative pressure state, but due to the obstruction and isolation of the filler in the drum 1, the air pressure inside the drum 1 is in a normal state, resulting in an imbalance in the pressure inside and outside the drum 1, and the air pressure inside the drum 1 will be released to the outside of the drum 1, at this time, the protein liquid will be squeezed out of the drum 1, which has the effect of accelerating the filtration of the protein liquid.

[0032] Furthermore, the motor 6 is placed above the exhaust device 7, with the output end of the motor 6 facing the inner wall of the box 31, and the output end is engaged with the transmission gear 314 in the gear assembly 311, providing power for the gear assembly 311, further driving the gear 122 to rotate, so that the drum 1 rotates.

[0033] Furthermore, a limiting block 313 is provided at the inner bottom of the box body 31 , and the concave groove formed by the limiting block 313 is used to place and limit the waste liquid collection box 4 .

[0034] It is worth mentioning that the drum 1 in the utility model is placed inside the box 31. The drum 1 can be taken out by opening the front window 32. At the same time, the drum 1 is formed by the barrel 11 and the barrel cover 12 being fastened and connected, and the two can be separated or fastened and combined. When the filtering work is finished, the front window 32 is opened, the drum 1 is taken out, and the barrel cover 12 is unscrewed to take out the filler. After the protein in the filler is extracted and washed, since the filler is a C18 bonded silica gel filler, the chemical properties are stable, so it can be used normally after washing. Similarly, the filler is placed in the drum, the front window 32 is closed, and the next filtering work can be carried out. Here, the detachable drum structure and the characteristics of the filler itself are cleverly combined to realize the recycling of the filler. At the same time, the structural design is convenient for taking the filler, which is convenient for the circulation work and avoids the waste of resources.

[0035] Specifically, the device of the utility model is used as follows:

[0036] like Figure 1-6, protein liquid is injected by liquid injection pipe 21 upper ends, after liquid injection pipe 21, straight pipe, flows into cylinder 1, and now, protein, salt and water are arranged in protein liquid.After protein liquid is injected, the rotating handle of rotary valve 22 is rotated, so that it is in a completely closed state, and the front viewing window 32 is closed simultaneously to make sealing, and this process is the preparatory stage before filtering work. Further, by control assembly 5, motor 6 and air extraction device 7 are worked, and the inside of the device enters the negative pressure state, and cylinder 1 is powered by motor 6, starts to rotate, and namely starts to filter, and protein liquid is placed in cylinder 1 inside, contacts with filler by inner cylinder 113 filter holes, and the protein in protein liquid is retained by filler, and salt and water are filtered and flow into the waste liquid collecting box 4 below cylinder via outer cylinder 114 filter holes again.Meanwhile, can observe by visual window 32, judge whether filtering has been completed, and this process is filtering process. Further, after the filtration is completed, the motor 6 and the air extraction device 7 are turned off by the control component 5, and the normal air pressure inside the device is restored. At this moment, there are only water and salt in the protein solution, and the protein is retained in the filler. Then the front window 32 is opened, the drum 1 is taken out, the drum cover 12 is opened, the filler and protein are taken out, and eluted with a suitable organic solvent, that is, the extraction of protein from the protein solution is completed. At the same time, the extracted filler is cleaned and put back into the drum 1, and can be used continuously, thereby achieving the purpose of recycling.

[0037] The above is only a preferred embodiment of the present invention. It should be pointed out that ordinary technicians in this technical field can make several improvements and supplements without departing from the principle of the present invention. These improvements and supplements should also be regarded as the protection scope of the present invention.

Claims

1. A drum-type protein desalination device, comprising a housing (31) and a drum (1), characterized in that: A roller (1) with a horizontal central axis is arranged in the box (31); The drum (1) is composed of a drum body (11) and a drum cover (12); The cylinder body (11) comprises an inner cylinder (113) and an outer cylinder (114); the bottoms of the inner cylinder (113) and the outer cylinder (114) are fixed to each other, and the top openings are in the same direction and flush; there is a gap between the inner cylinder (113) and the outer cylinder (114), and a filler is placed in the gap; and filtering holes are evenly distributed on the surfaces of the inner cylinder (113) and the outer cylinder (114); The opening side of the cylinder body (11) is connected to the cylinder cover (12).

2. The drum-type protein desalting device according to claim 1, characterized in that: The cross section of the gap in the vertical direction is annular, and a plurality of isolation blocks (115) are nested and inserted into the gap at equal intervals in the horizontal direction. The isolation blocks (115) divide the gap into a plurality of placement chambers, and the filler is placed in the placement chambers.

3. The drum-type protein desalting device according to claim 1, characterized in that: The cylinder cover (12) comprises a cylinder cover body (123), wherein two layers of cylindrical protrusions and gears (122) are provided at the center of one side of the cylinder cover body (123), and a cylindrical block (121) is also provided on the same side surface.

4. The drum-type protein desalting device according to claim 3, characterized in that: A convex block (111) is provided on the outside of the opening side of the barrel (11), and the convex block (111) is movably connected to a fastener (112), and the fastener (112) is buckled and connected to the cylindrical block (121).

5. The drum-type protein desalination device according to claim 3, characterized in that: A waste liquid collection box (4), a motor (6) and a gear assembly (311) are arranged inside the box body (31); the waste liquid collection box (4) is placed at the bottom of the box body (31), and the roller (1) is placed directly above the waste liquid collection box (4); in the horizontal direction, the cross-sectional area of ​​the waste liquid collection box (4) is larger than the cross-sectional area of ​​the roller (1).

6. The drum-type protein desalting device according to claim 5, characterized in that: The output end of the motor (6) is connected to one end of the gear assembly (311) and meshes with a transmission gear (314) in the gear assembly (311); the other end of the gear assembly (311) meshes with a gear (122) through the transmission gear (314); the gear (122) forms a whole with the drum (1) through the drum cover (12).

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