Auxiliary device for taking photos of elisa plate away from light under fluorescence microscope

By installing a light-blocking device and a foldable light-blocking strip on the enzyme label, the problem that the enzyme label plate under a fluorescence microscope cannot clearly confirm the target channel in a light-proof environment is solved, and the accuracy of the experimental results and the operator's convenient interpretation is achieved. .

CN222280472UActive Publication Date: 2024-12-31SHANGHAI DIAN CLINICAL TESTING CENT
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Patent Information

Application Number
CN202420461704.8
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-03-11
Publication Date
2024-12-31
Estimated Expiration
2034-03-11

AI Technical Summary

Technical Problem

The target channel cannot be clearly confirmed in a light-proof environment under a fluorescence microscope, which affects the interpretation of experimental results.

Method used

A light-proof photo auxiliary device for enzyme label board including multiple light-proof panel devices and foldable light-proof strips is designed. Using black hard plastic light-proof strips and labels, the physical light-proofing method prevents strong light or excitation light from affecting the experimental results, and through Foldable light shielding strips quickly locate the enzyme marking hole.

Benefits of technology

Effectively prevent the impact of strong light or excitation light on experimental results, ensuring the accuracy of experimental results and the operator's convenient interpretation.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model provides an elisa plate light-shielding photographing auxiliary device under a fluorescence microscope, and relates to the technical field of elisa plate light-shielding photographing auxiliary devices, the elisa plate light-shielding photographing auxiliary device comprises an elisa plate body, the bottom side of the elisa plate body is provided with a plurality of light shielding plate devices, the bottom of the elisa plate body is connected with a mounting plate II, and the mounting plate II is provided with a light shielding plate. Eight foldable shading strips are pasted on one side of the second mounting plate, and labels are pasted on one sides of the eight foldable shading strips. When an experiment is carried out, the mounting plate I and the shading strip can be moved to the two sides of an enzyme label hole needing to be observed for shading, so that the influence of strong light or exciting light on an experiment result can be prevented; each equally-divided foldable shading strip is marked with a label, rapid positioning can be achieved, the foldable shading strips corresponding to the labels can be turned over during observation positioning so as to rapidly position the corresponding enzyme label holes to be observed, and an operator can conveniently conduct observation operation.
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Description

Technical Field

[0001] The utility model relates to the technical field of light-shielding photography auxiliary devices for ELISA plates, in particular to a light-shielding photography auxiliary device for ELISA plates under a fluorescence microscope. Background Art

[0002] ELISA plates are common experimental consumables in the design of ELISA experiments. For experiments involving ELISA plates in laboratories, ELISA readers or fluorescence microscopes are usually used to interpret the experimental results. ELISA plates are divided into detachable and non-detachable ones. For non-detachable ones, the strips on the entire plate are connected together, while detachable ones are separated from the strips on the ELISA plate. The specifications of the separated strips are 12 holes and 8 holes. Removable ELISA plates are usually used during experimental operations to ensure the maximum utilization of the test kit.

[0003] In the prior art, black ELISA plates are now available in the market to protect ELISA plates from light, so that the results of ELISA instruments that need to be protected from light are more accurate. However, fluorescence microscopes require that the ELISA plates are transparent and the excitation light can penetrate them to ensure that the experimental results can be observed under the microscope. At this time, it is not advisable to modify the color of the ELISA plate itself, and in a light-proof environment, the operator cannot clearly confirm the target channel. Utility Model Content

[0004] The purpose of the utility model is to solve the problems existing in the prior art.

[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: an auxiliary device for light-shielding photography of ELISA plates under a fluorescence microscope, comprising: an ELISA plate body, a plurality of shading plate devices are arranged on the bottom side of the ELISA plate body, a mounting plate 2 is connected to the bottom of the ELISA plate body, eight foldable shading strips are pasted on one side of the mounting plate 2, and labels are pasted on one side of the eight foldable shading strips.

[0006] As a preferred embodiment, a plurality of enzyme-labeled holes are arranged on the surface of the enzyme-labeled plate body.

[0007] As a preferred embodiment, the shading plate device includes a mounting plate 1, the length of the mounting plate 1 is consistent with the ELISA plate body, the two ends of the mounting plate 1 are movably connected to the two sides of the bottom of the ELISA plate body, and a shading strip is fixedly installed on the bottom side of the mounting plate 1.

[0008] As a preferred embodiment, the two ends of the second mounting plate are movably connected to the two sides of the bottom of the ELISA plate body, and the second mounting plate is divided into eight equal parts corresponding to the ELISA holes on the ELISA plate body.

[0009] As a preferred implementation, the eight foldable shading strips correspond to eight equal parts of the mounting plate 2, and the eight equal parts of the foldable shading strips can be folded.

[0010] As a preferred embodiment, the surfaces of the eight labels are marked with numbers from one to eight, respectively, and the eight labels correspond to eight ELISA wells on the ELISA plate body.

[0011] Compared with the prior art, the advantages and positive effects of the utility model are:

[0012] 1. The utility model adopts a physical shading method. The material of the shading strip is black hard plastic. The two ends of the mounting plate are movably connected to the movable grooves on both sides of the bottom of the ELISA plate body. When conducting experiments, the mounting plate and the shading strip can be moved to both sides of the ELISA hole to be observed for shading, which can prevent strong light or excitation light from affecting the experimental results.

[0013] 2. In the utility model, the mounting plate is divided into eight equal parts or adjusted accordingly according to the number of enzyme label strips. The foldable shading strip of each equal part is labeled with a sequence and can be quickly positioned. The foldable shading strip is fixedly connected to one side of the mounting plate by hot melt adhesive or other glue and can be folded along the mounting edge. When observing and positioning, the foldable shading strip corresponding to the label can be folded to quickly locate the corresponding enzyme label hole to be observed, which is convenient for the operator to interpret the results. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 A schematic diagram of the main body of a light-shielding photography auxiliary device for ELISA plates under a fluorescence microscope provided by the utility model;

[0015] Figure 2 A structural diagram of a light shielding plate device of a light shielding photography auxiliary device for an ELISA plate under a fluorescence microscope provided by the utility model;

[0016] Figure 3 A structural diagram of a foldable light-shielding strip of an auxiliary device for light-shielding ELISA plates for photographing under a fluorescence microscope provided by the utility model.

[0017] Legend:

[0018] 1. ELISA plate body; 101. ELISA hole; 2. shading plate device; 201. Mounting plate 1; 202. shading strip; 3. Mounting plate 2; 4. Foldable shading strip; 5. Label. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0020] See also Figure 1-3 The utility model provides a technical solution: an auxiliary device for light-shielding photography of ELISA plates under a fluorescence microscope, comprising: an ELISA plate body 1, a plurality of shading plate devices 2 are arranged on the bottom side of the ELISA plate body 1, a mounting plate 2 3 is connected to the bottom of the ELISA plate body 1, eight foldable shading strips 4 are pasted on one side of the mounting plate 2 3, and labels 5 are pasted on one side of the eight foldable shading strips 4.

[0021] Specifically: the size of the shading plate device 2 is installed in different quantities according to the size of the ELISA plate body 1. When taking fluorescent photos, the shading plate device 2 can hold the maximum number minus one, and the remaining column can be used to hold the installable plate 2 3. The foldable shading strip 4 can be folded, and the numbers on the labels 5 on the foldable shading strip 4 are arranged from top to bottom. The ELISA hole 101 needs to be photographed for positioning.

[0022] In one embodiment, a plurality of ELISA wells 101 are disposed on the surface of the ELISA plate body 1 .

[0023] Specifically: the sample to be observed is placed in the enzyme labeling well 101 .

[0024] In one embodiment, the shading plate device 2 includes a mounting plate 201, the length of the mounting plate 201 is consistent with the ELISA plate body 1, the two ends of the mounting plate 201 are movably connected to the two sides of the bottom of the ELISA plate body 1, and a shading strip 202 is fixedly installed on the bottom side of the mounting plate 201.

[0025] Specifically: the mounting plate 201 is installed on the movable groove at the bottom of the ELISA plate body 1 and can move left and right. In particular, the two sides of the ELISA hole 101 to be photographed need to be strictly protected from light. The shading strip 202 is made of black hard plastic material to achieve a shading effect.

[0026] In one embodiment, two ends of the second mounting plate 3 are movably connected to two sides of the bottom of the ELISA plate body 1 , and the second mounting plate 3 is divided into eight equal parts corresponding to the ELISA holes 101 on the ELISA plate body 1 .

[0027] Specifically: the surface of the second installation plate 3 is divided into eight equal parts, each of which is provided with a foldable shading strip 4 corresponding to eight ELISA holes 101 in a row on the surface of the ELISA plate body 1 .

[0028] In one embodiment, eight foldable shading strips 4 correspond to eight equal parts of the mounting plate 2 3 , and the eight equal parts of the foldable shading strips 4 can be folded.

[0029] Specifically: the foldable shading strip 4 can be folded and made of black plastic. The folding angle can be adjusted to 180°. It is lifted up when photographing the corresponding enzyme-labeled hole 101, and the rest of the position is covered to prevent fluorescence quenching. If the foldable shading strip 4 is folded for a long time, the enzyme-labeled hole 101 may not be covered. After use, the foldable shading strip 4 can be folded back to restore the folding force of the foldable shading strip 4.

[0030] In one embodiment, the surfaces of the eight labels 5 are marked with numbers from one to eight, respectively, and the eight labels 5 correspond to eight ELISA wells 101 on the ELISA plate body 1 .

[0031] Specifically: the eight labels 5 are marked with 1-8 from top to bottom in order, that is, the labels 5 are in order, and the second mounting plate 3 and the foldable shading strip 4 cannot be reversed.

[0032] Working principle: It is a necessary experimental condition to avoid light in the fluorescent microscope shooting environment. In the light-avoiding experiment, the fluorescence quenching of the experimental object will inevitably occur when the strong light or excitation light is irradiated, which affects the experimental results. This device adopts the physical shading method. The material of the shading strip 202 is selected as black hard plastic. The two ends of the mounting plate 1 201 are movably connected in the movable grooves on both sides of the bottom of the ELISA plate body 1. When conducting an experiment, the mounting plate 1 201 and the shading strip 202 can be moved to both sides of the ELISA hole 101 to be observed to perform shading, which can prevent strong light or excitation light from affecting the experimental results; the mounting plate 2 3 is divided into eight equal parts or adjusted accordingly according to the number of ELISA strips. The foldable shading strip 4 of each equal part is marked with a label 5, which can be quickly positioned. The foldable shading strip 4 is fixedly connected to one side of the mounting plate 2 3 by hot melt glue or other glue, and can be folded along the mounting edge. When observing and positioning, the foldable shading strip 4 corresponding to the label 5 can be folded to quickly locate the corresponding ELISA hole 101 to be observed, which is convenient for the operator to observe and operate.

[0033] The above description is only a preferred embodiment of the present invention and does not limit the present invention in other forms. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes and apply it to other fields. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A light-shielding photography auxiliary device for ELISA plates under a fluorescence microscope, characterized in that: include: An ELISA plate body (1) is provided with a plurality of shading plate devices (2) on the bottom side of the ELISA plate body (1), a second mounting plate (3) is connected to the bottom of the ELISA plate body (1), eight foldable shading strips (4) are attached to one side of the second mounting plate (3), and labels (5) are attached to one side of the eight foldable shading strips (4).

2. The light-shielding photography auxiliary device for ELISA plates under a fluorescence microscope according to claim 1, characterized in that: The surface of the ELISA plate body (1) is provided with a plurality of ELISA holes (101).

3. The light-shielding photography auxiliary device for ELISA plates under a fluorescence microscope according to claim 1, characterized in that: The shading plate device (2) comprises a mounting plate (201), the length of which is consistent with that of the ELISA plate body (1), the two ends of which are movably connected to the two sides of the bottom of the ELISA plate body (1), and a shading strip (202) is fixedly installed on the bottom side of the mounting plate (201).

4. The light-shielding photography auxiliary device for ELISA plates under a fluorescence microscope according to claim 1, characterized in that: The two ends of the second mounting plate (3) are movably connected to the two sides of the bottom of the ELISA plate body (1), and the second mounting plate (3) is divided into eight equal parts corresponding to the ELISA holes (101) on the ELISA plate body (1).

5. The light-shielding photography auxiliary device for ELISA plates under a fluorescence microscope according to claim 1, characterized in that: The eight foldable shading strips (4) correspond to eight equal parts of the mounting plate 2 (3), and the eight equal parts of the foldable shading strips (4) can be folded.

6. The light-shielding photography auxiliary device for ELISA plates under a fluorescence microscope according to claim 1, characterized in that: The surfaces of the eight labels (5) are marked with numbers from one to eight, respectively, and the eight labels (5) correspond to eight ELISA holes (101) on the ELISA plate body (1).