Device suitable for culturing biofilm bacteria

Abstract

The utility model belongs to the technical field of culture devices, and discloses a device suitable for culturing biofilm bacteria, which comprises a metal bath box, a culture room and a pump set, an aluminum seat with an electric heating wire is arranged in the metal bath box, the culture room is embedded in the aluminum seat, and the pump set is arranged in the culture room. A fresh culture medium sample inlet, a uniform mixing inlet, a uniform mixing outlet, a waste liquid outlet, an air vent and four envelope sampling ports are formed in a sealing cover at the top of the culture chamber, an envelope sampling rod with more than two envelope bacterium retention blocks is mounted in each envelope sampling port, and the pump set is connected with the fresh culture medium sample inlet, the uniform mixing inlet, the uniform mixing outlet and the waste liquid outlet. A transparent protective cover is mounted at the top of the metal bath box; the aluminum seat with the heating wire is matched with the culture chamber, so that the temperature is more stable, bacterial culture is facilitated, the shearing force and the retention time are convenient to control and the culture medium is also convenient to mix uniformly through the matching of the uniform mixing inlet and the uniform mixing outlet with the peristaltic pump B, and the envelope sampling rod is arranged, so that sampling is facilitated.

Description

Technical Field

[0001] The utility model relates to a device suitable for culturing biofilm bacteria, belonging to the technical field of culturing devices. Background Art

[0002] Biofilm bacteria show different phenotypes from suspended bacterial cells of the same genotype. Different shear forces and residence times are required to induce bacteria to produce biofilms. The biofilm reactors currently used at home and abroad are: A. DFR (Drip Flow Reactor) reactor, which has a low flow rate and is only suitable for simulating a small shear environment; B. RDR (Rotating Dise Reactor) reactor, which quantitatively detects biofilm growth, but the shear force changes with the rotation speed; C. BAR (Biofilm Annular Reactor) reactor, which is complex to operate and easy to be polluted. The above reactors are not easy to control in terms of shear force and residence time, and are not systematic. They need to use various external equipment to conduct experiments. The operation is complicated and it is difficult to achieve experimental results. Therefore, a device suitable for culturing biofilm bacteria that is easy to control the shear force and residence time is needed. Utility Model Content

[0003] The utility model aims to provide a device suitable for culturing biofilm bacteria.

[0004] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a device suitable for culturing biofilm bacteria, comprising a metal bath, a culture chamber and a pump group, wherein an aluminum seat with a concave upper surface is installed in the metal bath, and an electric heating wire is embedded in the inner wall of the aluminum seat, and the culture chamber is slidably sleeved in the aluminum seat, and the top of the culture chamber extends to above the upper surface of the metal bath and is overlapped with a sealing cover, wherein nine openings are opened on the sealing cover, including a fresh culture medium inlet, a mixing inlet, a mixing outlet, a waste liquid outlet, a vent and four film sampling ports, a film sampling rod is spirally connected in the film sampling port, and the film sampling rod comprises an upper solid that is adapted to the film sampling port. A fixed block and a lower plate inserted into the culture chamber, more than two membrane bacteria retention blocks are installed on the lower plate, the sealing cover is fixedly connected to the top of the metal bath box through the cover clamping bolts rotatably connected to the side thereof, the pump group comprises a peristaltic pump A, a peristaltic pump B and a peristaltic pump C, the inlet end of the peristaltic pump A is connected to the fresh culture medium storage device, and the outlet end is connected to the fresh culture medium inlet port through the fresh culture medium inlet pipe, the outlet end of the peristaltic pump B is connected to the mixing inlet through the circulation inlet pipe, and the inlet end is connected to the mixing outlet through the circulation outlet pipe, the peristaltic pump C is connected to the waste liquid outlet through the waste liquid discharge pipe, and a controller is installed on the front of the metal bath box.

[0005] Preferably, the culture chamber is a U-shaped glass container, the sealing cover is made of 316L or 304 stainless steel, and a silicone sealing ring is fixedly connected to the lower surface of the sealing cover.

[0006] Preferably, the tops of the fresh culture medium inlet, the mixing inlet, the mixing outlet, the waste liquid outlet, and the vent are respectively connected with Luer connectors.

[0007] Preferably, the lower end of the fresh culture medium inlet is fixedly connected to a culture medium feed pipe extending into the lower part of the culture chamber, the lower end of the mixing inlet is fixedly connected to a circulation feed pipe extending into the lower part of the culture chamber, the lower end of the mixing outlet is fixedly connected to a circulation discharge pipe extending into the middle part of the culture chamber, and the lower end of the waste liquid discharge outlet is fixedly connected to a drainage pipe.

[0008] Preferably, the culture medium feed pipe, circulation feed pipe, circulation discharge pipe and drainage pipe are made of stainless steel.

[0009] Preferably, the lower end of the circulating feed pipe is connected to a spherical or ellipsoidal hole cover.

[0010] Preferably, the upper fixed block is a cylindrical block, a sealing ring is fixedly connected to the upper part of the outer wall of the upper fixed block, an external screw is provided at the lower part of the outer wall of the upper fixed block, and more than two mounting holes are provided on the side of the lower plate, and a membrane bacteria retention block is movably connected in the mounting hole.

[0011] Preferably, an arc-shaped silicone elastic limiting block is fixedly connected to the inner wall of the mounting hole, and a limiting groove is provided on the outer wall of the film bacteria retention block.

[0012] Preferably, the upper fixing block and the lower plate of the membrane sampling rod are made of stainless steel, and the membrane bacteria retention block is made of polycarbonate, stainless steel, PP or frosted glass.

[0013] Preferably, the controller is a touch screen single chip microcomputer controller, and electronic modules such as a computing board and a temperature control system are arranged inside the controller. A transparent protective cover is arranged on the top of the metal bath box.

[0014] Beneficial Effects

[0015] The utility model is suitable for culturing biofilm bacteria. The device forms a heating module by cooperating an aluminum seat with a heating wire and a culture chamber, so that the ambient temperature is more stable, and the temperature of the culture chamber is also more stable, which is convenient for bacterial culture. The cooperation of a long and a short circulating feed pipe and a circulating discharge pipe with a peristaltic pump B facilitates the control of the shear force and the residence time, and also facilitates the mixing of the culture medium. The provision of a biofilm sampling rod that is easy to disassemble and assemble facilitates sampling. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1It is a schematic diagram of the structure of the utility model;

[0017] Figure 2 It is a structural schematic diagram of the culture chamber in the utility model;

[0018] Figure 3 It is a top view of the sealing cover in the utility model;

[0019] Figure 4 It is a structural schematic diagram of the membrane sampling rod in the utility model.

[0020] In the figure: 1. Metal bath; 2. Culture chamber; 3. Pump group; 4. Sealing cover; 5. Fresh culture medium inlet; 6. Mixing inlet; 7. Mixing outlet; 8. Waste liquid outlet; 9. Vent; 10. Film sampling port; 11. Film sampling rod; 12. Upper fixing block; 13. Lower plate; 14. Film bacteria retention block; 15. Cover clamping bolt; 16. Fresh culture medium inlet pipeline; 17. Circulation inlet pipeline; 18. Circulation outlet pipeline; 19. Waste liquid discharge pipe; 20. Controller; 21. Culture medium feed pipe; 22. Circulation feed pipe; 23. Circulation discharge pipe; 24. Drain pipe; 25. Sealing ring; 26. External screw; 27. Transparent protective cover. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] like Figure 1-4As shown, the utility model provides a technical solution: a device suitable for culturing biofilm bacteria, comprising a metal bath 1, a culture chamber 2 and a pump group 3, an aluminum seat (aluminum base) with a concave upper surface is installed in the metal bath 1, an inner wall of the aluminum seat is embedded with an electric heating wire, the culture chamber 2 is slidably sleeved in the aluminum seat, the outer wall of the culture chamber 2 is slidably fitted with the inner wall of the aluminum seat, the aluminum seat with the heating wire and the culture chamber 2 form a heating module, so that the ambient temperature is more stable, and the temperature of the culture chamber 2 is more stable and the temperature rises faster, the top of the culture chamber 2 extends to above the upper surface of the metal bath 1, and is overlapped with a sealing cover 4, the sealing cover 4 is provided with nine openings that penetrate the sealing cover 4 from the thickness direction, including a fresh culture medium inlet 5 with stirring function, a mixing inlet 6, a mixing outlet 7, and a waste liquid discharge outlet 8. , vent 9 and four membrane sampling ports 10, the lower end of the fresh culture medium inlet 5 is fixedly connected to a culture medium feed pipe 21 extending into the lower part of the culture chamber 2, the lower end of the mixing inlet 6 is fixedly connected to a circulation feed pipe 22 extending into the lower part of the culture chamber 2, and the lower end of the mixing outlet 7 is fixedly connected to a circulation discharge pipe 23 extending into the middle part of the culture chamber 2. The long and short settings of the circulation feed pipe 22 and the circulation discharge pipe 23 are convenient for mixing the culture medium, and are convenient for controlling the stop time and shear force when combined with the peristaltic pump B. The lower end of the circulation feed pipe 22 is connected to a spherical or ellipsoidal hole cover, which is convenient for stirring the culture using the power generated by the injection. The lower end of the waste liquid discharge port 8 is fixedly connected to a discharge pipe 24. The vent 9 is externally connected to a precision filter. The precision filter in this embodiment is 0.22um filter, can pass oxygen, air, 10% carbon dioxide gas, so as to be suitable for the growth of different bacteria, the membrane sampling port 10 is spirally connected with a membrane sampling rod 11, the membrane sampling rod 11 includes an upper fixing block 12 adapted to the membrane sampling port 10 and a lower plate 13 inserted into the culture chamber 2, the upper fixing block 12 and the lower plate 13 are integrally formed, and more than two membrane bacteria retention blocks 14 are installed on the lower plate 13. In this embodiment, the number of membrane bacteria retention blocks 14 is four, and the sealing cover 4 is fixedly connected to the top of the metal bath box 1 through the cover clamping bolt 15 rotatably connected to the side of the sealing cover 4. The side of the sealing cover 4 is rotatably connected with the cover clamping bolt 15, and the lower end of the cover clamping bolt 15 is threadedly connected to the threaded hole opened on the top of the metal bath box 1, so that the sealing cover 4 is fixed to the top of the culture chamber 2, and the culture chamber 2 is fixed on the metal bath box 1 at the same time, so that the sealing cover 4 presses the culture chamber 2 body without damaging the culture chamber 2, which is convenient for installation, cleaning and sterilization, and the pump group 3 It includes peristaltic pump A, peristaltic pump B and peristaltic pump C. The inlet end of peristaltic pump A is connected to the fresh culture medium storage device, and the outlet end is connected to the fresh culture medium inlet 5 through the fresh culture medium inlet pipe 16. The outlet end of peristaltic pump B is connected to the mixing inlet 6 through the circulation inlet pipe 17, and the inlet end is connected to the mixing outlet 7 through the circulation outlet pipe 18. The stop time and shear force can be controlled. Peristaltic pump C is connected to the waste liquid outlet 8 through the waste liquid discharge pipe 19, and the discharge volume can be controlled. The setting is consistent with the peristaltic pump A for conveying fresh culture, so that the inlet and outlet are balanced. A controller 20 is installed on the front of the metal bath box 1. The controller 20 is a touch screen single-chip microcomputer controller 20. The controller 20 is provided with a computing board, temperature control and other electronic modules. The electric heating wire and the pump group 3 are electrically connected to the controller 20 and are controlled by the controller 20. A transparent protective cover 27 is provided on the top of the metal bath box 1, and the pump group 3 and the sealing cover 4 are both located in the transparent protective cover 27. .

[0023] Specifically, the culture chamber 2 is a glass container with a U-shaped longitudinal cross-section. In this embodiment, the volume of the culture chamber 2 is 1000 ml. The sealing cover 4 is made of 316L or 304 stainless steel. A silicone sealing ring 25 is fixedly connected to the lower surface of the sealing cover 4. The silicone sealing ring 25 fits tightly with the top opening of the culture chamber 2 to facilitate sealing with the culture chamber 2. The culture chamber 2 and the sealing cover 4 can be taken out as a whole for high-pressure steam sterilization.

[0024] Specifically, the tops of the fresh culture medium inlet 5, the mixing inlet 6, the mixing outlet 7, the waste liquid discharge outlet 8, and the vent 9 are respectively connected with Luer connectors, and the fresh culture medium inlet pipe 16, the mixing inlet pipe 17, the mixing outlet 7, the waste liquid discharge outlet 8 are respectively connected to the fresh culture medium inlet pipe 16, the circulation inlet pipe 17, the circulation outlet pipe 18, and the waste liquid discharge pipe 19 through the Luer connectors, and the vent 9 is connected to the precision filter through the Luer connector. The Luer connector in this embodiment is a stainless steel Luer quick connector, and the fresh culture medium inlet pipe 16, the circulation inlet pipe 17, and the circulation outlet pipe 18 are sterile catheters.

[0025] Specifically, the culture medium feed pipe 21, the circulation feed pipe 22, the circulation discharge pipe 23 and the drainage pipe 24 are made of stainless steel to facilitate high-pressure steam sterilization.

[0026] Specifically, the upper fixed block 12 is a cylindrical block, and the upper part of the outer wall of the upper fixed block 12 is fixedly connected with a sealing ring 25 that is compatible with the membrane sampling port 10, and the lower part of the outer wall of the upper fixed block 12 is provided with an external screw thread 26 that is compatible with the internal thread provided on the inner wall of the membrane sampling port 10. The side of the lower plate 13 is provided with more than two mounting holes that penetrate the lower plate 13 from the thickness direction, and the membrane bacteria retention block 14 is movably connected in the mounting hole. In this embodiment, the number of mounting holes is four, and the number of membrane bacteria retention blocks 14 is correspondingly four. An arc-shaped silicone elastic limit block is fixedly connected to the inner wall of the mounting hole, and a limit groove that is compatible with the arc-shaped silicone elastic limit block is provided on the outer wall of the membrane bacteria retention block 14. The upper fixed block 12 and the lower plate 13 of the membrane sampling rod 11 are made of stainless steel to facilitate high-pressure steam sterilization, and the membrane bacteria retention block 14 is made of polycarbonate, stainless steel, PP or frosted glass.

[0027] The working principle of the utility model is as follows: first, the membrane sampling rod 11 is installed on the sealing cover 4, the culture chamber 2 and the sealing cover 4 are sterilized at high temperature and high pressure, and several liters of fresh culture medium are prepared at the same time. The specific preparation amount is determined according to the experimental plan, and it is sterilized for standby use. The device is powered on, and the controller 20 controls the electric heating wire on the aluminum seat in the metal bath box 1 to heat up, and the inner wall of the aluminum seat is preheated. Then, the culture chamber 2 is placed in the aluminum seat, and then the sealing cover 4 is covered on the top opening of the culture chamber 2, and the cover clamping bolts 15 are tightened to fix the sealing cover 4 above the metal bath box 1, thereby sealing the culture chamber 2, and then the fresh culture medium inlet 5 on the sealing cover 4 is connected to the peristaltic pump A of the pump group 3, and the mixing inlet 6 and the mixing outlet 7 are connected to the peristaltic pump The inlet and outlet ends of B are connected, the waste liquid discharge port 8 is connected to the peristaltic pump C, and the vent 9 is externally connected to a precision filter, which can pass oxygen, air, and 10% carbon dioxide gas to suit the growth of different bacteria. Then the parameter process can be set according to the experimental plan to culture the bacteria. The utility model is suitable for culturing biofilm bacteria. The device forms a heating module through the cooperation of an aluminum seat with a heating wire and a culture chamber, so that the ambient temperature is more stable, and the temperature of the culture chamber is also more stable, which is convenient for bacterial culture. Through the cooperation of a long and a short circulating feed pipe and a circulating discharge pipe with the peristaltic pump B, it is convenient to control the shear force and the residence time, which is convenient for inducing bacteria to produce a film and also convenient for mixing the culture. The film sampling rod that is easy to disassemble and assemble is provided to facilitate sampling.

Claims

1. A device suitable for culturing biofilm bacteria, comprising a metal bath (1), a culture chamber (2) and a pump unit (3), characterized in that: An aluminum seat with a concave upper surface is installed in the metal bath box (1), and an electric heating wire is embedded in the inner wall of the aluminum seat. The culture chamber (2) is embedded in the aluminum seat. The top of the culture chamber (2) extends to the upper surface of the metal bath box (1) and is overlapped with a sealing cover (4). The sealing cover (4) is provided with nine openings, including a fresh culture medium inlet (5), a mixing inlet (6), a mixing outlet (7), a waste liquid outlet (8), a vent (9) and four membrane sampling ports (10). A membrane sampling rod (11) is spirally connected to the membrane sampling port (10). The membrane sampling rod (11) includes an upper fixing block (12) adapted to the membrane sampling port (10) and a lower plate (13) inserted into the culture chamber (2). The lower plate (13) has an upper More than two membrane bacteria retention blocks (14) are installed, and the sealing cover (4) is fixedly connected to the top of the metal bath box (1) through a cover clamping bolt (15) that is rotatably connected to the side thereof. The pump group (3) includes a peristaltic pump A, a peristaltic pump B and a peristaltic pump C. The inlet end of the peristaltic pump A is connected to a fresh culture medium storage device, and the outlet end is connected to a fresh culture medium inlet port (5) through a fresh culture medium inlet pipe (16). The outlet end of the peristaltic pump B is connected to a mixing inlet (6) through a circulation inlet pipe (17), and the inlet end is connected to a mixing outlet (7) through a circulation outlet pipe (18). The peristaltic pump C is connected to a waste liquid outlet (8) through a waste liquid outlet pipe (19). A controller (20) is installed on the front of the metal bath box (1).

2. A device for culturing biofilm bacteria according to claim 1, characterized in that: The culture chamber (2) is a U-shaped glass container, the sealing cover (4) is made of 316L or 304 stainless steel, and a silicone sealing ring (25) is fixedly connected to the lower surface of the sealing cover (4).

3. A device for culturing biofilm bacteria according to claim 1, characterized in that: The tops of the fresh culture medium inlet (5), the mixing inlet (6), the mixing outlet (7), the waste liquid outlet (8), and the vent (9) are respectively connected with Luer connectors, and the fresh culture medium inlet pipe (16), the circulation inlet pipe (17), and the circulation outlet pipe (18) are sterile conduits.

4. A device for culturing biofilm bacteria according to claim 1, characterized in that: The lower end of the fresh culture medium inlet (5) is fixedly connected to a culture medium feed pipe (21) extending into the lower part of the culture chamber (2); the lower end of the mixing inlet (6) is fixedly connected to a circulation feed pipe (22) extending into the lower part of the culture chamber (2); the lower end of the mixing outlet (7) is fixedly connected to a circulation discharge pipe (23) extending into the middle part of the culture chamber (2); and the lower end of the waste liquid discharge port (8) is fixedly connected to a discharge pipe (24).

5. A device for culturing biofilm bacteria according to claim 4, characterized in that: The culture medium feed pipe (21), the circulating feed pipe (22), the circulating discharge pipe (23) and the liquid discharge pipe (24) are made of stainless steel.

6. A device for culturing biofilm bacteria according to claim 4, characterized in that: The lower end of the circulating feed pipe (22) is connected with a spherical or ellipsoidal hole cover.

7. The device for culturing biofilm bacteria according to claim 1, characterized in that: The upper fixed block (12) is a cylindrical block, the upper portion of the outer wall of the upper fixed block (12) is fixedly connected to a sealing ring (25), the lower portion of the outer wall of the upper fixed block (12) is provided with an external screw (26), and the side surface of the lower plate (13) is provided with more than two mounting holes, and the membrane bacteria retention block (14) is movably connected in the mounting hole.

8. A device for culturing biofilm bacteria according to claim 7, characterized in that: An arc-shaped silicone elastic limiting block is fixedly connected to the inner wall of the installation hole, and a limiting groove is provided on the outer wall of the membrane bacteria retention block (14).

9. The device for culturing biofilm bacteria according to claim 1, characterized in that: The fixed block (12) and the lower plate (13) of the membrane sampling rod (11) are made of stainless steel, and the membrane bacteria retention block (14) is made of polycarbonate, stainless steel, PP or frosted glass.

10. The device for culturing biofilm bacteria according to claim 1, characterized in that: The controller (20) is a touch screen type single chip microcomputer controller (20), a computing board and a temperature control electronic module are arranged inside the controller (20), and a transparent protective cover (27) is arranged on the top of the metal bath box (1).

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