SOD (superoxide dismutase) purification reaction kettle

Abstract

The utility model relates to the technical field of reaction kettles, in particular to an SOD (superoxide dismutase) purification reaction kettle which comprises a reaction kettle body, a reaction chamber is arranged in the reaction kettle body, a sealed cooling cavity is formed between the reaction chamber and the side wall of the reaction kettle body, and a feeding hole is formed in one side of the top of the reaction kettle body. A cooling water inlet is formed below the side wall of the reaction kettle body, a cooling water outlet is formed above the side wall of the reaction kettle body, a bottom cover is in threaded connection with the bottom of the reaction kettle body, a discharge port is formed in the bottom of the bottom cover, a first valve is arranged on the discharge port, a discharge pipe is in threaded connection with the bottom of the discharge port, and a filter screen is arranged at the top end of the discharge pipe. A temperature sensor and a heating device are also arranged in the reaction kettle body. According to the device, heat change of impure protein is achieved by controlling the temperature in the reaction kettle body, the structure of the impure protein is destroyed, and condensation and precipitation are conducted, so that SOD is purified, the bottom cover and the discharging pipe at the bottom of the device are convenient to disassemble and assemble, and meanwhile the interior of the bottom cover and the interior of the discharging pipe are convenient to clean.

Description

Technical Field

[0001] The utility model relates to the technical field of reaction kettles, in particular to a SOD purification reaction kettle. Background Art

[0002] Superoxide dismutase (SOD for short) is an enzyme that catalyzes the dismutation reaction of superoxide anions, and is also a metalloenzyme widely present in animals, plants and microorganisms. SOD has the functions of anti-aging, anti-tumor, anti-radiation and enhancing human immunity, and is known as the scavenger of garbage in the human body. Currently, it is mainly used to delay human aging, prevent pigmentation, eliminate local inflammation, and is particularly used to treat rheumatoid arthritis, chronic polyarthritis and inflammation after radiotherapy. It has no antigenicity and less toxic and side effects, and is a therapeutic enzyme with great clinical value. Moreover, in recent years, SOD has become an important additive in daily chemical products and health food industries.

[0003] The preparation method of SOD varies with the raw materials. At present, the raw materials for preparing SOD at home and abroad include animal blood, microorganisms and animal and plant tissues. There are a lot of impurities in the SOD in the production process of the prior art. With the increase in people's demand for SOD, higher requirements are put forward for the SOD purification process. In view of this, we propose a SOD purification reactor. Utility Model Content

[0004] The utility model aims to provide a SOD purification reaction kettle to better remove foreign proteins in SOD and improve the purity of SOD.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A SOD purification reactor comprises a reactor body, wherein a reaction chamber is arranged inside the reactor body, a sealed cooling cavity is formed between the reaction chamber and the side wall of the reactor body, a feed port is arranged on one side of the top of the reactor body, a cooling water inlet is arranged below the side wall of the reactor body, a cooling water outlet is arranged above the side wall of the reactor body, a bottom cover is threadedly connected to the bottom of the reactor body, a discharge port is arranged at the bottom of the bottom cover, a first valve is arranged on the discharge port, a discharge pipe is threadedly connected to the bottom of the discharge port, a filter screen is arranged at the top of the discharge pipe, and a temperature sensor and a heating device are also arranged inside the reactor body.

[0007] Preferably, a motor is provided on the top of the reactor body, and a stirring device is provided inside the reaction chamber. The stirring device comprises a rotating shaft and a stirring paddle. The output end of the motor is fixedly connected to one end of the rotating shaft, and the other end of the rotating shaft is fixedly connected to the stirring paddle.

[0008] Preferably, a bracket is fixedly installed at the bottom of the side wall of the reactor body, and the bracket includes a support ring and a plurality of support columns. The support ring is fixedly sleeved on the bottom of the outer side wall of the reactor body, and the plurality of support columns are evenly arranged below the support ring, and the plurality of support columns are fixedly connected to the support ring above.

[0009] Preferably, a second valve is provided on the cooling water inlet and the cooling water outlet.

[0010] Preferably, a temperature display screen is provided on the outer wall of the reactor body, and the temperature display screen is electrically connected to a temperature sensor.

[0011] Preferably, a filter membrane is laid on the filter screen, and the filter membrane only allows SOD molecules to pass through.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] 1. The SOD purification reactor is provided with a heating device and a cooling chamber, and the temperature inside the reaction chamber is monitored by a temperature sensor and a temperature display. By controlling the temperature inside the reactor body, the impurity protein is thermally changed, and the structure of the impurity protein is destroyed and condensed and precipitated, while SOD still maintains its original activity. SOD is separated from the impurity protein by a filter screen and a filter membrane, so that SOD is purified.

[0014] 2. The bottom of the SOD purification reactor is threadedly connected to a bottom cover, a discharge port is arranged at the bottom of the bottom cover, and a discharge pipe is threadedly connected to the bottom of the discharge port, so that the bottom cover and the discharge pipe can be easily disassembled and installed, and the bottom cover and the discharge pipe can be easily cleaned. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the internal structure of the reactor body in the utility model;

[0017] Figure 3 This is a schematic diagram of the structure of the discharge pipe in the utility model.

[0018] In the figure: 1. Reactor body; 2. Reaction chamber; 3. Cooling chamber; 4. Feed inlet; 5. Cooling water inlet; 51. Second valve; 6. Cooling water outlet; 7. Bottom cover; 8. Discharge port; 81. First valve; 9. Discharge pipe; 10. Filter; 11. Temperature sensor; 12. Heating device; 13. Motor; 14. Stirring device; 141. Rotating shaft; 142. Stirring paddle; 15. Bracket; 151. Support ring; 152. Support column; 16. Temperature display screen. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0020] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0021] In the description of this patent, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", and "set" should be understood in a broad sense, for example, it can be fixedly connected or set, or it can be detachably connected or set, or connected or set in one piece. For ordinary technicians in this field, the specific meanings of the above terms in this patent can be understood according to specific circumstances.

[0022] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present utility model, the meaning of "several" is two or more, unless otherwise clearly and specifically defined.

[0023] See also Figure 1-Figure 3 As shown, a technical solution provided by the utility model:

[0024] A SOD purification reactor comprises a reactor body 1, wherein a reaction chamber 2 is arranged inside the reactor body 1, a sealed cooling cavity 3 is formed between the reaction chamber 2 and the side wall of the reactor body 1, a feed port 4 is arranged on one side of the top of the reactor body 1, a cooling water inlet 5 is arranged below the side wall of the reactor body 1, a cooling water outlet 6 is arranged above the side wall of the reactor body 1, a bottom cover 7 is threadedly connected to the bottom of the reactor body 1, a discharge port 8 is arranged at the bottom of the bottom cover 7, a first valve 81 is arranged on the discharge port 8, a second valve 51 is arranged on the cooling water inlet 5 and the cooling water outlet 6, a discharge pipe 9 is threadedly connected to the bottom of the discharge port 8, a filter screen 10 is arranged at the top of the discharge pipe 9, a temperature sensor 11 and a heating device 12 are further arranged inside the reactor body 1, and the reaction chamber 2 inside the reactor body 1 is heated by the heating device 12 to denature the impurity protein by heat.

[0025] In this embodiment, in order to make the liquid in the reaction chamber 2 heated evenly, a motor 13 is arranged on the top of the reactor body 1, and a stirring device 14 is arranged inside the reaction chamber 2. The stirring device 14 includes a rotating shaft 141 and a stirring paddle 142. The output end of the motor 13 is fixedly connected to one end of the rotating shaft 141, and the other end of the rotating shaft 141 is fixedly connected to the stirring paddle 142. The rotating shaft 141 is controlled to rotate by the motor 13, thereby driving the stirring paddle 142 to rotate, and the liquid in the reaction chamber 2 is stirred. The liquid is heated evenly, the impurity protein is thermally denatured, and the purity of SOD is improved.

[0026] In this embodiment, in order to fix the reactor body 1, a bracket 15 is fixedly provided at the bottom of the side wall of the reactor body 1, and the bracket 15 includes a support ring 151 and a plurality of support columns 152. The support ring 151 is fixedly sleeved on the bottom of the outer wall of the reactor body 1, and the plurality of support columns 152 are evenly arranged below the support ring 151, and the plurality of support columns 152 are fixedly connected to the support ring 151 above.

[0027] In this embodiment, a temperature display screen 16 is disposed on the outer wall of the reactor body 1 . The temperature display screen 16 is electrically connected to the temperature sensor 11 . The temperature display screen 16 can display the real-time temperature inside the reaction chamber 2 .

[0028] As an implementation mode of this embodiment, a filter membrane is laid on the filter screen 10, and the filter membrane only allows SOD molecules to pass through. The heat-changed impurities remain on the bottom cover 7 of the reactor body 1, and the SOD solution flows out from the discharge pipe 9 through the filter membrane and the filter screen 10.

[0029] When the SOD purification reactor of the present embodiment is used, the SOD liquid to be purified is added from the feed port 4 to the reaction chamber 2 inside the reactor body 1, the inside of the reaction chamber 2 is heated by the heating device 12, the temperature inside the reaction chamber 2 is measured by the temperature sensor 11, and the temperature inside the reaction chamber 2 can be monitored in real time by the cooperation of the temperature display screen 16. The temperature inside the reaction chamber 2 is first controlled to be maintained at 45°C-55°C to make the impurities undergo thermal changes, and then the second valve 51 of the cooling water inlet 5 and the cooling water outlet 6 is opened, and the flow is introduced into the cooling chamber 3. The reaction chamber 2 is cooled by cooling water to reduce the temperature inside the reaction chamber 2 to room temperature, and the temperature inside the reaction chamber 2 is monitored by the temperature sensor 11 and the temperature display screen 16. The structure of the impurity protein is destroyed and condensed and precipitated. Then the first valve 81 of the discharge port 8 is opened, and the SOD solution flows out from the discharge pipe 9 through the filter membrane and the filter screen 10, and the impurity protein precipitate remains in the bottom cover 7 above the filter membrane. The bottom cover 7 is threadedly connected to the reactor body 1 and the discharge pipe 9 respectively, which is convenient for disassembly and installation of the bottom cover 7 and the discharge pipe 9, and also convenient for cleaning the bottom cover 7 and the inside of the discharge pipe 9.

[0030] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the utility model and are not used to limit the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection of the utility model is defined by the attached claims and their equivalents.

Claims

1. A SOD purification reactor, comprising a reactor body (1), characterized in that: A reaction chamber (2) is arranged inside the reactor body (1), a sealed cooling chamber (3) is formed between the reaction chamber (2) and the side wall of the reactor body (1), a feed port (4) is arranged on one side of the top of the reactor body (1), a cooling water inlet (5) is arranged below the side wall of the reactor body (1), a cooling water outlet (6) is arranged above the side wall of the reactor body (1), a bottom cover (7) is threadedly connected to the bottom of the reactor body (1), a discharge port (8) is arranged at the bottom of the bottom cover (7), a first valve (81) is arranged on the discharge port (8), a discharge pipe (9) is threadedly connected to the bottom of the discharge port (8), a filter screen (10) is arranged at the top of the discharge pipe (9), and a temperature sensor (11) and a heating device (12) are also arranged inside the reactor body (1).

2. The SOD purification reactor according to claim 1, wherein: A motor (13) is arranged on the top of the reactor body (1), and a stirring device (14) is arranged inside the reaction chamber (2). The stirring device (14) comprises a rotating shaft (141) and a stirring paddle (142). The output end of the motor (13) is fixedly connected to one end of the rotating shaft (141), and the other end of the rotating shaft (141) is fixedly connected to the stirring paddle (142).

3. The SOD purification reactor according to claim 1, characterized in that: A bracket (15) is fixedly arranged at the bottom of the side wall of the reactor body (1), and the bracket (15) comprises a support ring (151) and a plurality of support columns (152). The support ring (151) is fixedly sleeved on the bottom of the outer side wall of the reactor body (1), the plurality of support columns (152) are evenly arranged below the support ring (151), and the plurality of support columns (152) are fixedly connected to the support ring (151) above.

4. The SOD purification reactor according to claim 1, characterized in that: The cooling water inlet (5) and the cooling water outlet (6) are provided with a second valve (51).

5. The SOD purification reactor according to claim 1, characterized in that: The outer wall of the reactor body (1) is provided with a temperature display screen (16), and the temperature display screen (16) is electrically connected to the temperature sensor (11).

6. The SOD purification reactor according to claim 1, characterized in that: The filter screen (10) is provided with a filter membrane, and the filter membrane only allows SOD molecules to pass through.

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