Anti-falling DNA probe processing device

Abstract

The utility model discloses an anti-drop DNA probe processing device which comprises a pedestal and a portal frame, an X-axis motor is assembled on the left side above the pedestal, an output shaft of the X-axis motor is fixedly connected with a linear screw rod, the linear screw rod is sleeved with a threaded seat, the front end of the threaded seat is fixedly connected with a vertical frame, and the vertical frame is fixedly connected with the portal frame. The front end of the vertical frame is fixedly connected with a fixing plate. According to the anti-falling DNA probe treatment device, the pipetting frame, the pipetting heads, the fixing plate, the injection needle tube and the hanging frame are arranged, the hanging frame is communicated with the multiple sets of pipetting heads to be hung to the bottom end of the pipetting frame together, during hanging, the top end of each set of pipetting head is aligned with the injection needle tube, the multiple sets of pipetting heads are fixed through the hanging frame, the multiple sets of pipetting heads can be replaced at a time, and the operation is convenient. The pipetting heads are replaced in batches on the premise of not causing pollution, the problem that a single pipetting head is frequently replaced to cause loose connection and fall off is solved, and the problem that the pipetting heads are easy to fall off is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of DNA probe processing, in particular to a DNA probe processing device which is anti-shedding. Background Art

[0002] In the field of DNA probe processing, the application of DNA probe processing devices has greatly improved the efficiency and accuracy of DNA probe packaging. Compared with the traditional manual packaging method, this automated device has significant advantages. The automated device can accurately control the amount of liquid dispensed each time, ensuring the consistency and reliability of experimental results.

[0003] According to the application number CN201721672468.0, a precise packaging and mixing device for DNA primers or probes is disclosed, which is mainly composed of a control cabinet, an actuator, a working area component, a test unit, a gun tip rack, a work table and a fixed table; the actuator is composed of an X-axis slide, a Y-axis slide, a Z-axis slide and a gun axis slide; the working area components include 8 packaging target plates, 1 gun tip plate, 1 stock liquid plate, 1 liquid guide target plate and 1 gun tip separator, and the work table adopts a layout with the stock liquid plate as the center.

[0004] However, in the above technical solution, the pipette tip that needs to be replaced is fixed to the main head, and the connection between the pipette tip and the needle is not stable enough. After a group of samples are aspirated, it needs to be disassembled and replaced, which easily causes the connection to loosen. When disassembling, a special pipette separator is required, but the direct connection fixing method causes certain wear on the main head every time it is separated and reassembled. The wear caused by frequent disassembly and replacement during high-speed operation often causes the pipette tip to loosen.

[0005] Therefore, there is an urgent need for an anti-shedding DNA probe processing device to solve the technical defects proposed in the above-mentioned technology. Utility Model Content

[0006] The utility model aims to provide a DNA probe processing device that prevents falling off, so as to solve the problem in the above background technology that frequent disassembly and replacement during high-speed operation often causes the pipette tip to loosen due to wear.

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a DNA probe processing device that prevents falling off, comprising a pedestal and a gantry, an X-axis motor is installed on the left side above the pedestal, the output shaft of the X-axis motor is fixedly connected to a linear screw, the outer side of the linear screw is sleeved with a threaded seat, the front end of the threaded seat is fixedly connected to a vertical frame, the front end of the vertical frame is fixedly connected to a fixed plate, the bottom end of the fixed plate is fixed with a lifting cylinder, the output shaft of the lifting cylinder is provided with a push rod, the bottom end of the push rod is fixedly connected to a pipetting stand, two groups of injection cylinders are installed inside the pipetting stand, multiple groups of piston rods are fixed to the bottom end of the injection cylinders, multiple groups of injection needles are sleeved on the outside of the piston rods, the bottom end of the injection needles is sleeved with a pipetting head, a group of hanging racks are fixed to the outside of the pipetting head, and the hanging racks are hung and fixed to the pipetting stand.

[0008] Preferably, the pipette head has a diameter larger than that of the injection needle, and the top end of the injection needle is assembled in the pipette stand.

[0009] Preferably, the injection needle tubes are provided in multiple groups, and the injection needle tubes are arranged at equal intervals.

[0010] Preferably, a reserved groove is opened in the middle position inside the base, a group of transmission grooves are arranged on both sides of the reserved groove, and the transmission grooves are equipped with conveyor belts. A double-headed cylinder is fixed in the reserved groove, a group of side panels are fixed on both side edges of the base, a group of correction plates are fixed on one side of the side panels and the output end of the double-headed cylinder, an infrared reflective sheet is arranged at the correction plate, infrared sensors are arranged on both sides of the bottom end of the pipetting rack, and guide rods are fixedly connected in the reserved groove.

[0011] Preferably, two ends of the guide rod are respectively sleeved with a group of movable sleeves, and two sides of the movable sleeves are respectively hinged with hinge frames.

[0012] Preferably, a total of four groups of the articulated frames are provided, and the articulated frames form a diamond-shaped supporting structure.

[0013] Preferably, a group of corner wheels are installed at each of the four corners of the top of the fixed plate, a Z-axis motor is installed at one group of corner wheels, a group of belts are respectively sleeved on the outside of the corner wheels, a Z-axis moving plate is commonly sleeved at the middle position of the belts, and two groups of reserved wheels are installed on the top of the Z-axis moving plate and connected to the belts.

[0014] Preferably, a display is installed on the right side of the gantry to control the movement of motors and cylinders in various parts of the device.

[0015] Compared with the prior art, the utility model has the following beneficial effects: the anti-falling DNA probe processing device not only realizes the function of preventing the pipetting head from falling off and realizing the function of stabilizing the operation, but also realizes the function of protecting the probe;

[0016] (1) A pipette stand, a pipette head, a fixing plate, an injection cylinder, a piston rod, an injection needle tube, and a hanging rack are provided. The injection needle tube mainly adopts an automatically driven liquid aspiration method. First, the hanging rack is connected to multiple groups of pipette heads and hung together to the bottom of the pipette stand. When hanging, the top of each group of pipette heads is aligned with the injection needle tube. After assembly, the injection cylinder pushes the piston rod downward to expel the air in the injection needle tube and the pipette head. When the pipette head moves to the right orifice plate for liquid aspiration and dripping, the injection cylinder pushes multiple groups of piston rods to move downward in the injection needle tube at the same time. The DNA experimental liquid absorbed in the pipette head is dripped into the left orifice plate by air thrust. Multiple groups of pipette heads are fixed by the hanging rack. Multiple groups of pipette heads can be replaced at one time. The amount of liquid absorbed is determined by the lifting distance of the piston rod. The liquid will not spread into the injection needle tube. The pipette heads can be replaced in batches without causing pollution, and the problem of loose connection and falling off caused by frequent replacement of a single pipette head can be prevented. The pipette head has the function of preventing the pipette head from falling off;

[0017] (2) Angle wheels, a Z-axis moving plate, a belt, and a fixed plate are provided. At the fixed plate, a set of angle wheels is driven to rotate by a Z-axis motor, and multiple sets of angle wheels are driven to rotate together by the belt, so as to drive the Z-axis moving plate to move linearly along the Y-axis, and drive the pipetting rack to move above the well plate. The belt and the angle wheel are driven in a linked manner to reduce the load and avoid the axial force affecting the stability of the movement, thereby ensuring that one or more rows of injection needles can be stably installed and stably operated, which has the effect of stabilizing the operation;

[0018] (3) A conveyor belt, a double-headed cylinder, an infrared sensor, and an infrared reflective sheet are provided. Two groups of orifice plates are placed on the conveyor belt, one group of which contains DNA liquid, which needs to be sucked up by a syringe and dripped into the other group. Before sucking, the double-headed cylinder drives two groups of correction plates located on the inside to clamp the orifice plate to fix the position of the orifice plate. The infrared sensor emits infrared light to the infrared reflective sheet to detect whether the orifice plate is in place, and then the probe sucking operation is performed to ensure that the pipette head can be stably inserted into the tube of the orifice plate without touching the edge to cause deviation, operation failure, and probe damage, thereby having a probe protection effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a front view cross-sectional structural schematic diagram of the utility model;

[0020] Figure 2 This is a schematic diagram of the top view structure of the fixing plate of the utility model;

[0021] Figure 3 This is a front view structural diagram of the pipetting stand of the utility model;

[0022] Figure 4 This is a schematic diagram of the top view of the pedestal structure of the utility model;

[0023] Figure 5It is a schematic diagram of the top view structure of the correction plate of the utility model.

[0024] In the figure: 1. base; 2. transmission trough; 3. transmission belt; 4. side plate; 5. correction plate; 6. display; 7. gantry; 8. linear screw; 9. threaded seat; 10. vertical frame; 11. X-axis motor; 12. Z-axis motor; 13. lifting cylinder; 14. push rod; 15. pipetting stand; 16. infrared sensor; 17. pipetting head; 18. fixed plate; 19. injection cylinder; 20. piston rod; 21. injection needle; 22. hanging rack; 23. angle wheel; 24. Z-axis moving plate; 25. belt; 26. guide rod; 27. double-head cylinder; 28. infrared reflector; 29. ​​movable sleeve; 30. articulated frame. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] Example 1: Please refer to Figure 1-5 A DNA probe processing device that prevents falling off includes a pedestal 1 and a gantry 7. An X-axis motor 11 is installed on the left side above the pedestal 1. The output shaft of the X-axis motor 11 is fixedly connected to a linear screw 8. The linear screw 8 is sleeved with a threaded seat 9 on the outside. The front end of the threaded seat 9 is fixedly connected to a vertical frame 10. The front end of the vertical frame 10 is fixedly connected to a fixed plate 18. The bottom end of the fixed plate 18 is fixed with a lifting cylinder 13. The output shaft of the lifting cylinder 13 is provided with a push rod 14. The bottom end of the push rod 14 is fixedly connected to a pipetting rack 15. Two sets of injections are installed inside the pipetting rack 15. The injection cylinder 19 has a plurality of piston rods 20 fixed at the bottom end of the injection cylinder 19, and a plurality of injection needle tubes 21 are sleeved on the outside of the piston rods 20. The bottom end of the injection needle tube 21 is sleeved with a pipetting head 17, and a group of hangers 22 are fixed on the outside of the pipetting head 17. The hangers 22 are fixedly connected to the pipetting rack 15. The pipetting head 17 has a larger diameter than the injection needle tube 21. The top of the injection needle tube 21 is assembled in the pipetting rack 15. There are a plurality of injection needle tubes 21, and the injection needle tubes 21 are arranged at equal intervals. A display 6 is installed on the right side of the gantry 7 to control the movement of motors and cylinders at various locations of the device.

[0027] Specifically, Figure 1 and Figure 3As shown, the injection needle 21 mainly adopts an automatically driven liquid suction method. First, the hanger 22 is connected to multiple groups of pipetting heads 17 and hung together to the bottom of the pipetting rack 15. When hanging, the top of each group of pipetting heads 17 is aligned with the injection needle 21. After assembly, the injection cylinder 19 pushes the piston rod 20 downward to discharge the air in the injection needle 21 and the pipetting head 17. When the pipetting head 17 moves to the right orifice plate for liquid suction and dripping operations, the injection cylinder 19 pushes multiple groups of piston rods 20 to move downward in the injection needle 21 at the same time, and the DNA experimental liquid absorbed in the pipetting head 17 is dripped into the left orifice plate through air thrust. Multiple groups of pipetting heads 17 are fixed by the hanger 22, and multiple groups of pipetting heads 17 can be replaced at one time. The liquid suction amount is determined by the lifting distance of the piston rod 20. The liquid will not spread to the injection needle 21. The pipetting heads 17 are replaced in batches without causing pollution, preventing the problem of loose connection and falling off caused by frequent replacement of a single pipetting head 17.

[0028] Embodiment 2: A set of corner wheels 23 are installed at each of the four corners of the top of the fixed plate 18, and a Z-axis motor 12 is installed at one set of corner wheels 23. A set of belts 25 are respectively sleeved on the outside of the corner wheels 23, and a Z-axis moving plate 24 is sleeved in the middle of the belts 25. Two sets of reserved wheels are installed on the top of the Z-axis moving plate 24 and connected to the belts 25;

[0029] Specifically, Figure 1 and Figure 2 As shown, at the fixed plate 18, a group of corner wheels 23 are driven to rotate by the Z-axis motor 12, and multiple groups of corner wheels 23 are driven to rotate together by the belt 25, driving the Z-axis movable plate 24 to move linearly along the Y-axis, driving the pipette rack 15 to move above the well plate, and using the belt 25 and the corner wheel 23 to drive in a linked manner to reduce the load and avoid the axial force affecting the stability of the movement, thereby ensuring that one or more rows of injection needles 21 can be stably installed and operated.

[0030] Embodiment 3: A reserved groove is provided in the middle position inside the pedestal 1, a group of transmission grooves 2 are arranged on both sides of the reserved groove, and a transmission belt 3 is installed in each transmission groove 2. A double-headed cylinder 27 is fixed in the reserved groove, a group of side panels 4 are fixed on the edges of both sides of the pedestal 1, a group of correction plates 5 are fixed on one side of the side panels 4 and the output end of the double-headed cylinder 27, an infrared reflection sheet 28 is arranged at the correction plate 5, infrared sensors 16 are arranged on both sides of the bottom of the pipetting rack 15, a guide rod 26 is fixedly connected in the reserved groove, and a group of movable sleeves 29 are sleeved on both ends of the outer side of the guide rod 26, and hinged frames 30 are respectively hinged on both sides of the movable sleeve 29, and there are four groups of hinged frames 30 in total, and the hinged frames 30 constitute a rhombus support structure;

[0031] Specifically, Figure 1 , Figure 4 and Figure 5As shown, on the conveyor belt 3, two groups of well plates are placed on the left and right sides, one group contains DNA liquid, which needs to be sucked by the injection needle 21 and dripped into the other group. Before sucking, the two groups of correction plates 5 located on the inside are driven by the double-headed cylinder 27 to clamp the well plates to fix the position of the well plates, and the infrared sensor 16 emits infrared light to the infrared reflective sheet 28 to detect that the well plates are in place, and then the probe sucking operation is performed to ensure that the pipette head 17 can be stably inserted into the tube of the well plate without touching the edge to cause deviation, operation failure and probe damage.

[0032] Working principle: The staff places the multi-well plates on two groups of conveyor belts 3 in a row, with an empty well plate on the left and a well plate containing DNA liquid on the right. The two groups of correction plates 5 located on the inside are driven by the double-headed cylinder 27 to clamp the well plates to fix the position of the well plates. The infrared sensor 16 emits infrared light to the infrared reflective sheet 28 to detect that the well plates are in place, and then the probe suction operation is performed. During the operation, the X-axis motor 11 drives the pipetting rack 15 to move left and right, and the Z-axis motor 12 drives a group of corner wheels 23 to rotate. The belt 25 drives multiple groups of corner wheels 23 to rotate together, and drives the Z-axis moving plate 24 to move back and forth to perform liquid suction and dripping operations. After completing the DNA operation of one group of well plates, the conveyor belt 3 drives backward to perform the next group of operations, and the staff takes away the two groups of well plates that have completed the operation and puts new well plates in front. Since the amount of DNA liquid absorbed is determined by the lifting distance of the piston rod 20, the liquid will not spread to the injection needle 21. Therefore, the pipetting heads 17 can be replaced in batches without causing pollution.

[0033] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A DNA probe processing device that prevents falling off, comprising a base (1) and a gantry (7), characterized in that: An X-axis motor (11) is mounted on the left side above the pedestal (1), the output shaft of the X-axis motor (11) is fixedly connected to a linear screw (8), the outer portion of the linear screw (8) is sleeved with a threaded seat (9), the front end of the threaded seat (9) is fixedly connected to a vertical frame (10), the front end of the vertical frame (10) is fixedly connected to a fixed plate (18), the bottom end of the fixed plate (18) is fixed with a lifting cylinder (13), the output shaft of the lifting cylinder (13) is provided with a push rod (14), The bottom end of the push rod (14) is fixedly connected to a pipetting rack (15), two groups of injection cylinders (19) are installed inside the pipetting rack (15), multiple groups of piston rods (20) are fixed to the bottom end of the injection cylinders (19), multiple groups of injection needle tubes (21) are sleeved on the outside of the piston rods (20), a pipetting head (17) is sleeved on the bottom end of the injection needle tubes (21), a group of hanging racks (22) are fixed on the outside of the pipetting head (17), and the hanging racks (22) are hung and fixed to the pipetting rack (15).

2. The anti-dropping DNA probe processing device according to claim 1, characterized in that: The pipetting head (17) has a diameter larger than that of the injection needle (21), and the top end of the injection needle (21) is assembled in the pipetting rack (15).

3. The anti-dropping DNA probe processing device according to claim 1, characterized in that: The injection needle tubes (21) are provided in multiple groups, and the injection needle tubes (21) are arranged at equal intervals.

4. The anti-dropping DNA probe processing device according to claim 1, characterized in that: A reserved groove is provided in the middle of the base (1), a group of transmission grooves (2) are arranged on both sides of the reserved groove, a transmission belt (3) is installed in each of the transmission grooves (2), a double-headed cylinder (27) is fixed in the reserved groove, a group of side plates (4) are fixed on the edges of both sides of the base (1), a group of correction plates (5) are fixed on one side of the side plate (4) and the output end of the double-headed cylinder (27), an infrared reflection sheet (28) is arranged on the correction plate (5), infrared sensors (16) are arranged on both sides of the bottom end of the pipetting rack (15), and a guide rod (26) is fixedly connected in the reserved groove.

5. The DNA probe processing device according to claim 4, characterized in that: The two ends of the guide rod (26) are respectively sleeved with a group of movable sleeves (29), and both sides of the movable sleeve (29) are respectively hinged with hinge frames (30).

6. The DNA probe processing device according to claim 5, characterized in that: A total of four groups of the articulated frames (30) are provided, and the articulated frames (30) form a rhombus-shaped supporting structure.

7. The DNA probe processing device according to claim 1, characterized in that: A group of corner wheels (23) are respectively installed at the four corners of the top of the fixed plate (18), and a Z-axis motor (12) is installed at one of the corner wheels (23). A group of belts (25) are respectively sleeved on the outside of the corner wheels (23), and a Z-axis moving plate (24) is sleeved in the middle of the belts (25). Two groups of reserved wheels connected to the belts (25) are installed on the top of the Z-axis moving plate (24).

8. The anti-dropping DNA probe processing device according to claim 1, characterized in that: A display (6) is installed on the right side of the gantry (7) to control the movement of motors and cylinders at various locations of the device.

Images