Cryopreserved cell recovery device

Abstract

The utility model discloses a cryopreserved cell recovery device and relates to the technical field of cryopreserved cell recovery. The water bath device comprises a water bath box, a pipe placing assembly and a transmission assembly, the pipe placing assembly comprises a mounting ring and a placing disc, the mounting ring is arranged in the water bath box, the outer side face of the mounting ring is rotationally connected with a rotating frame, one end of the rotating frame is fixedly connected with the inner wall of the water bath box, and the interior of the mounting ring is fixedly connected with a connecting rod. By arranging the pipe containing assembly and the transmission assembly, the driving motor is started, the cam is driven to rotate through the fixing rod, the cam can push the containing disc to do reciprocating motion in the horizontal direction under the cooperation of the sliding block and the connecting rod while rotating, the inner gear disc is driven to rotate through the driving gear, and then the mounting ring is driven to rotate; a plurality of groups of test tubes with cryopreserved cells are fixed in the clamping holes of the placing disc, so that the plurality of groups of test tubes can reciprocate in the water body in the shell, and the thawing efficiency of the cryopreserved cells in the test tubes is improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of cryopreserved cell resuscitation, in particular to a cryopreserved cell resuscitation device. Background Art

[0002] Cell cryopreservation and thawing is one of the cell culture techniques. Cell cryopreservation refers to freezing cells in liquid nitrogen for long-term storage, and cryopreserved cell thawing refers to reviving frozen cells and restoring their vitality. For cryopreserved cell thawing, the faster the thawing speed, the better, so that larger ice crystals will not be re-formed inside and outside the cells, and they will not be exposed to high-concentration electrolyte solutions for too long, so that no ice crystal damage and solute damage will occur. The frozen cells will still maintain their normal structure and function after thawing; currently, a water bath is required for thawing when thawing frozen cells.

[0003] Currently, in cell recovery, cells are usually frozen by hand and stirred quickly in a 37°C to 39°C water bath. Only one cell cryopreservation tube can be recovered each time, which is not convenient for large-scale recovery of frozen cells. Utility Model Content

[0004] The utility model aims to provide a cryopreserved cell resuscitation device to solve the problem that only a single cell cryopreservation tube can be resuscitated during cell resuscitation, which is inconvenient for resuscitation of a large number of cryopreserved cells.

[0005] In order to solve the above technical problems, the utility model is realized by the following technical solutions:

[0006] The utility model discloses a frozen cell resuscitation device, comprising a water bath, a tube placement assembly and a transmission assembly, wherein the tube placement assembly comprises a mounting ring and a placement plate, wherein the mounting ring is arranged inside the water bath, the outer side surface of the mounting ring is rotatably connected with a rotating frame, one end of the rotating frame is fixedly connected with the inner wall of the water bath, the inside of the mounting ring is fixedly connected with a connecting rod, two groups of connecting rods are arranged, the placement plate is arranged on the upper surface of the mounting ring, both sides of the bottom surface of the placement plate are fixedly connected with sliders, the two groups of sliders are respectively arranged on the outsides of the two groups of connecting rods and slidably cooperate with them, the two side surfaces of the sliders are fixedly connected with limiting springs, and one end of the limiting spring is fixedly connected with the outer side surface of the connecting rod.

[0007] A rectangular groove is provided at the center of the upper surface of the placement plate, and the transmission assembly includes a driving motor and a fixing rod. The driving motor is installed on one side of the bottom surface of the water bath, and the fixing rod is arranged in the water bath and rotatably cooperates with the water bath. One end of the fixing rod is fixedly connected to the rotating shaft of the driving motor, and the other end of the fixing rod is fixedly connected to a cam. The cam is arranged in the rectangular groove, and one side surface of the cam is in close contact with the inner side surface of the rectangular groove.

[0008] The bottom surface of the mounting ring is fixedly connected with a mounting frame, the center position of the mounting frame is fixedly connected with an inner gear plate, the outer side of the fixing rod is fixedly connected with a driving gear, and the driving gear is arranged in the inner gear plate and meshed with it.

[0009] The upper surface of the placement plate is provided with a plurality of clamping holes, and the outer sides of the clamping holes are provided with rubber soft sleeves; by providing the clamping holes and the rubber soft sleeves, multiple groups of test tubes can be clamped and their shaking can be avoided.

[0010] A drain pipe is installed on one side of the bottom surface of the water bath.

[0011] The connection between the fixing rod and the drainage pipe and the water bath box is provided with a sealing sleeve.

[0012] The utility model has the following beneficial effects:

[0013] The utility model sets a tube placement assembly and a transmission assembly, starts a driving motor, and drives a cam to rotate through a fixing rod, so that while the cam rotates, with the cooperation of a slider and a connecting rod, it can push a placement plate to reciprocate in a horizontal direction, and drives an internal gear plate to rotate through a driving gear, thereby driving a mounting ring to rotate. Multiple groups of test tubes with frozen cells are fixed in the clamping holes of the placement plate, so that the multiple groups of test tubes can reciprocate in the water body in the shell, thereby increasing the thawing efficiency of the frozen cells in the test tubes.

[0014] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for describing the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0016] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0017] Figure 2 It is another structural schematic diagram of the utility model;

[0018] Figure 3 It is a front view of the structure of the utility model;

[0019] Figure 4 for Figure 3 Schematic diagram of the AA section structure.

[0020] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0021] 1. Water bath; 101. Drain pipe; 2. Tube placement assembly; 201. Mounting ring; 202. Placement plate; 203. Snap-in hole; 204. Rectangular groove; 205. Rotating frame; 206. Mounting frame; 207. Internal gear plate; 208. Slider; 3. Transmission assembly; 301. Cam; 302. Drive motor; 303. Fixed rod; 304. Drive gear; 305. Connecting rod; 306. Limiting spring. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] In the description of the present invention, it should be understood that the terms "upper", "middle", "outer", "inner" and the like indicating directions or positional relationships are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limitations on the present invention.

[0024] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0025] See also Figure 1-Figure 4As shown, the utility model is a frozen cell resuscitation device, including a water bath 1, a tube placement assembly 2 and a transmission assembly 3. The tube placement assembly 2 includes a mounting ring 201 and a placement plate 202. The mounting ring 201 is arranged inside the water bath 1. The outer side surface of the mounting ring 201 is rotatably connected to a rotating frame 205. One end of the rotating frame 205 is fixedly connected to the inner wall of the water bath 1. The interior of the mounting ring 201 is fixedly connected to a connecting rod 305. Two groups of connecting rods 305 are arranged. The placement plate 202 is arranged on the upper surface of the mounting ring 201. Both sides of the bottom surface of the placement plate 202 are fixedly connected with sliders 208. The two groups of sliders 208 are respectively arranged on the outsides of the two groups of connecting rods 305 and slidably cooperate with them. Both side surfaces of the slider 208 are fixedly connected with limiting springs 306. One end of the limiting spring 306 is fixedly connected to the outer side surface of the connecting rod 305.

[0026] A rectangular groove 204 is provided at the center of the upper surface of the placement plate 202. The transmission assembly 3 includes a driving motor 302 and a fixing rod 303. The driving motor 302 is installed on one side of the bottom surface of the water bath 1. The fixing rod 303 is arranged in the water bath 1 and rotates with the water bath 1. One end of the fixing rod 303 is fixedly connected to the rotating shaft of the driving motor 302, and the other end thereof is fixedly connected to a cam 301. The cam 301 is arranged in the rectangular groove 204, and one side surface of the cam 301 is in close contact with the inner side surface of the rectangular groove 204.

[0027] The bottom surface of the mounting ring 201 is fixedly connected with a mounting frame 206, the center position of the mounting frame 206 is fixedly connected with an inner gear plate 207, the outer side of the fixing rod 303 is fixedly connected with a driving gear 304, and the driving gear 304 is arranged in the inner gear plate 207 and meshed therewith.

[0028] A plurality of snap-in holes 203 are provided on the upper surface of the placement plate 202, and a rubber soft cover is provided on the outer side of the snap-in holes 203; by providing the snap-in holes 203 and the rubber soft cover, multiple groups of test tubes can be snap-in while preventing them from shaking.

[0029] A drain pipe 101 is installed on one side of the bottom surface of the water bath 1 .

[0030] The connection between the fixing rod 303 and the drain pipe 101 and the water bath 1 is provided with a sealing sleeve.

[0031] It should be noted that, when the technical solution is actually used, multiple groups of test tubes containing frozen cells are fixed in the snap-in holes 203 of the placement plate 202. The test tubes are prevented from shaking by the snap-in of the rubber sleeve. Hot water is poured into the water bath 1, and then the drive motor 302 is started. The cam 301 is driven to rotate through the fixing rod 303. When the cam 301 rotates, the placement plate 202 can be pushed to reciprocate in the horizontal direction with the cooperation of the slider 208 and the connecting rod 305. The inner gear plate 207 is driven to rotate through the driving gear 304, and then the mounting ring 201 is driven to rotate, so that multiple groups of test tubes can reciprocate in the water in the shell, thereby increasing the thawing efficiency of the frozen cells in the test tubes.

[0032] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0033] The preferred embodiments of the utility model disclosed above are only used to help explain the utility model. The preferred embodiments do not describe all the details in detail, nor do they limit the utility model to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that technicians in the relevant technical field can well understand and use the utility model. The utility model is only limited by the claims and their full scope and equivalents.

Claims

1. A frozen cell resuscitation device, comprising a water bath (1), a tube placement assembly (2) and a transmission assembly (3), characterized in that: The tube placement assembly (2) comprises a mounting ring (201) and a placement plate (202), wherein the mounting ring (201) is arranged inside the water bath (1), and the outer side surface of the mounting ring (201) is rotatably connected to a rotating frame (205), one end of the rotating frame (205) is fixedly connected to the inner wall of the water bath (1), and the interior of the mounting ring (201) is fixedly connected to a connecting rod (305), and two groups of connecting rods (305) are arranged, and the placement plate (202) is arranged on the upper surface of the mounting ring (201), and both sides of the bottom surface of the placement plate (202) are fixedly connected to sliders (208), and the two groups of sliders (208) are respectively arranged on the outer sides of the two groups of connecting rods (305) and slidably cooperate with them, and both sides of the sliders (208) are fixedly connected to limiting springs (306), and one end of the limiting spring (306) is fixedly connected to the outer side surface of the connecting rod (305).

2. A frozen cell resuscitation device according to claim 1, characterized in that: A rectangular groove (204) is provided at the center position of the upper surface of the placement plate (202), and the transmission assembly (3) includes a driving motor (302) and a fixing rod (303). The driving motor (302) is installed on one side of the bottom surface of the water bath (1), and the fixing rod (303) is arranged in the water bath (1) and rotates with the water bath (1). One end of the fixing rod (303) is fixedly connected to the rotating shaft of the driving motor (302), and the other end of the fixing rod (303) is fixedly connected to a cam (301). The cam (301) is arranged in the rectangular groove (204), and one side surface of the cam (301) is tightly attached to the inner side surface of the rectangular groove (204).

3. A frozen cell resuscitation device according to claim 2, characterized in that: The bottom surface of the mounting ring (201) is fixedly connected to a mounting frame (206), the center position of the mounting frame (206) is fixedly connected to an inner gear plate (207), the outer side of the fixing rod (303) is fixedly connected to a driving gear (304), and the driving gear (304) is arranged in the inner gear plate (207) and meshes therewith.

4. A frozen cell resuscitation device according to claim 3, characterized in that: A plurality of clamping holes (203) are provided on the upper surface of the placement plate (202), and a rubber soft sleeve is provided on the outer side of the clamping holes (203).

5. A frozen cell resuscitation device according to claim 4, characterized in that: A drainage pipe (101) is installed on one side of the bottom surface of the water bath (1).

6. A frozen cell resuscitation device according to claim 5, characterized in that: The connection points between the fixing rod (303) and the drainage pipe (101) and the water bath (1) are all provided with sealing sleeves.

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