Circulating tumor cell capturing and releasing chip

Abstract

The utility model discloses a chip for capturing and releasing circulating tumor cells, and relates to the technical field of biomedicine. The circulating tumor cell capturing and releasing chip comprises a bottom cover, a micro-fluidic chip attached to the upper surface of the bottom cover and a cover plate placed on the surface of the micro-fluidic chip, and an adjusting assembly is installed in the center of the interior of the bottom cover. By arranging the top connecting pipe, the bottom connecting pipe, the adjusting assembly and the like, the sealing plate can be driven to slide by rotating the bolt, the interiors of the first sealing groove and the second sealing groove can be in a negative pressure state, the top frame, the top cover and the bottom cover can be rapidly and fixedly assembled together, the detection efficiency of capturing circulating tumor cells is improved, and the detection cost is reduced. And by rotating the bolts, fixed-point stress can be uniform when the top frame, the top cover and the bottom cover are assembled, the problems that time and labor are wasted and stress is not uniform when traditional bolts are assembled are solved, and stability is enhanced.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedicine, in particular to a circulating tumor cell capture and release chip. Background Art

[0002] The circulating tumor cell capture and release chip is a microchip specifically designed to capture and separate circulating tumor cells (CTCs). Circulating tumor cells are a rare type of cancer cell that can float in the blood or body fluids and spread to other parts of the body through the blood circulation. The chip uses microfluidics technology and special surface coatings to identify, capture and separate circulating tumor cells in the blood. Once circulating tumor cells are captured, they can be further analyzed to understand information such as their characteristics and quantity, thereby helping doctors make more accurate cancer diagnosis and treatment decisions. In addition to capturing, the circulating tumor cell capture and release chip can also release captured cells so that they can be used for subsequent laboratory research and analysis. The use of this chip is widely used in cancer research and tumor progression monitoring, and can provide non-invasive tumor diagnosis and personalized treatment possibilities;

[0003] In the prior art, the components of the device for obtaining highly active circulating tumor cells are connected by bolts. When assembling by bolts, tools are needed to tighten each bolt, which is time-consuming and labor-intensive. In addition, when tightening, it is easy to cause uneven force and tilt and twist of the sealing gasket, which affects the sealing and the normal use of the device. Utility Model Content

[0004] In view of the deficiencies in the prior art, the utility model provides a circulating tumor cell capture and release chip, which solves the problem that the various detachable components of the existing device for obtaining highly active circulating tumor cells are fixedly connected by bolts. When assembling by bolts, tools are needed to tighten each bolt, which is time-consuming and labor-intensive. During tightening, it is easy to cause uneven force, tilt and twist of the sealing gasket, affect the sealing, and affect the normal use of the device.

[0005] To achieve the above objectives, the utility model is implemented through the following technical solutions: a circulating tumor cell capture and release chip, comprising a bottom cover, a microfluidic chip attached to the upper surface of the bottom cover, a cover plate placed on the surface of the microfluidic chip, a top cover placed on the surface of the cover plate, and a top frame placed on the surface of the top cover, the bottom of the top frame is fixedly connected with a top connecting pipe, the interior of the top cover is provided with a first sealing groove, the bottom of the top cover is fixedly connected with a bottom connecting pipe, the interior of the bottom cover is provided with a second sealing groove, and an adjustment component is installed at the inner center of the bottom cover;

[0006] The adjustment assembly includes a shell fixedly connected to the inside of the bottom cover, the inside of the shell is sealingly and slidably connected to a sealing plate, a threaded groove is provided at the inner center of the sealing plate, a bolt is meshing and rotatably connected inside the threaded groove, a positioning bearing is fixedly installed on the outer surface of the bolt, an air relief groove is provided on the surface of the shell, a connecting pipe is fixedly connected to the surface of the shell away from the bolt, one end of the connecting pipe away from the shell is connected to the inside of the second sealing groove, a limiting groove is provided inside the sealing plate, and a limiting rod is inserted into the inside of the limiting groove.

[0007] Preferably, the top connecting tube and the bottom connecting tube are both annular, and sealing rings are fixedly installed on the outer surfaces of the top connecting tube and the bottom connecting tube.

[0008] Preferably, the outer surface of the top connecting pipe is sealed and inserted into the interior of the first sealing groove, and the outer surface of the bottom connecting pipe is sealed and inserted into the interior of the second sealing groove.

[0009] Preferably, one end of the interior of the housing close to the connecting pipe is connected to the interior of the second sealing groove through the connecting pipe, and the outer surface of the locating bearing is fixedly installed inside the housing.

[0010] Preferably, the interior of the shell away from the connecting pipe is connected to the exterior of the shell through an air discharge groove.

[0011] Preferably, the number of the limiting rods and the limiting grooves is, one end of the two limiting rods away from the connecting tube is fixedly connected to the inner wall of the shell, and the surface of the microfluidic chip is modified with nucleic acid aptamers.

[0012] Beneficial Effects

[0013] The utility model provides a circulating tumor cell capture and release chip. Compared with the prior art, it has the following beneficial effects:

[0014] 1. The circulating tumor cell capture and release chip is provided with a top connecting tube, a bottom connecting tube and an adjustment component, etc., and can drive the sealing plate to slide by turning the bolts, so that the interior of the first sealing groove and the second sealing groove can be in a negative pressure state, and the top frame, the top cover and the bottom cover can be quickly fixed and assembled together, thereby improving the detection efficiency of circulating tumor cell capture and enhancing practicality.

[0015] 2. The circulating tumor cell capture and release chip can ensure uniform force at fixed points when assembling the top frame, top cover and bottom cover by turning the bolts, thus avoiding the time-consuming and labor-intensive traditional bolt assembly and uneven force problems and enhancing stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the structure of the utility model;

[0017] Figure 2 It is an exploded view of the utility model;

[0018] Figure 3 It is a cross-sectional view of the top cover in the utility model;

[0019] Figure 4 It is a cross-sectional view of the bottom cover of the utility model;

[0020] Figure 5 It is a structural schematic diagram of the adjustment component in the utility model.

[0021] In the figure: 1. bottom cover; 2. microfluidic chip; 3. cover plate; 4. top cover; 5. top frame; 6. top connecting pipe; 7. first sealing groove; 8. bottom connecting pipe; 9. second sealing groove; 10. adjustment component; 101. housing; 102. sealing plate; 103. threaded groove; 104. bolt; 105. positioning bearing; 106. air release groove; 107. connecting pipe; 108. limit groove; 109. limit rod. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0023] See also Figure 1-5 The utility model provides a technical solution: a circulating tumor cell capture and release chip, comprising a bottom cover 1, a microfluidic chip 2 attached to the upper surface of the bottom cover 1, a cover plate 3 placed on the surface of the microfluidic chip 2, a top cover 4 placed on the surface of the cover plate 3 and a top frame 5 placed on the surface of the top cover 4, a top connecting tube 6 is fixedly connected to the bottom of the top frame 5, a first sealing groove 7 is opened inside the top cover 4, the outer surface of the top connecting tube 6 is sealed and inserted in the first sealing groove 7, a bottom connecting tube 8 is fixedly connected to the bottom of the top cover 4, the top connecting tube 6 and the bottom connecting tube 8 are both annular, and sealing rings are fixedly installed on the outer surfaces of the top connecting tube 6 and the bottom connecting tube 8, a second sealing groove 9 is opened inside the bottom cover 1, the outer surface of the bottom connecting tube 8 is sealed and inserted in the second sealing groove 9, and an adjustment component 10 is installed at the inner center of the bottom cover 1, and the top connecting tube 6 and the second sealing groove 9 can be connected by setting the bottom connecting tube 8;

[0024] The surface of the microfluidic chip is modified with nucleic acid aptamers, which can recognize tumor marker proteins and be used to capture circulating tumor cells;

[0025] The adjustment assembly 10 includes a housing 101 fixedly connected to the inside of the bottom cover 1, the inside of the housing 101 is sealed and slidably connected to a sealing plate 102, a threaded groove 103 is provided at the inner center of the sealing plate 102, a bolt 104 is meshed and rotatably connected to the inside of the threaded groove 103, a positioning bearing 105 is fixedly installed on the outer surface of the bolt 104, the outer surface of the positioning bearing 105 is fixedly installed inside the housing 101, a venting groove 106 is provided on the surface of the housing 101, a connecting pipe 107 is fixedly connected to the surface of the housing 101 away from the bolt 104, the inside of the housing 101 away from the connecting pipe 107 is connected to the outside of the housing 101 through the venting groove 106, and one end of the inside of the housing 101 close to the connecting pipe 107 is connected to the second sealing The interior of the groove 9 is connected, and the end of the connecting pipe 107 away from the outer shell 101 is connected to the interior of the second sealing groove 9. A limiting groove 108 is provided inside the sealing plate 102, and a limiting rod 109 is inserted into the limiting groove 108. The number of limiting rods 109 and limiting grooves 108 is the same, and one end of the two limiting rods 109 away from the connecting pipe 107 is fixedly connected to the inner wall of the outer shell 101. By setting a top connecting pipe 6, a bottom connecting pipe 8 and an adjustment component 10, etc., by rotating the bolt 104, the sealing plate 102 can be driven to slide, so that the interior of the first sealing groove 7 and the second sealing groove 9 can be in a negative pressure state, and the top frame 5, the top cover 4 and the bottom cover 1 can be quickly fixed and assembled together, thereby improving the detection efficiency of circulating tumor cell capture and enhancing practicality.

[0026] During assembly, after placing the microfluidic chip 2 and the cover plate 3 at the center of the upper surface of the bottom cover 1, the top cover 4 is placed on the upper surface of the cover plate 3, and the bottom connecting tube 8 is inserted into the second sealing groove 9, and then the top frame 5 is placed on the upper surface of the top cover 4, and the top connecting tube 6 is inserted into the first sealing groove 7. By turning the bolt 104, the sealing plate 102 slides toward the direction close to the air release groove 106. At the same time, the second sealing groove 9, the bottom connecting tube 8, the first sealing groove 7 and the top connecting tube 6 are all in a negative pressure state. The assembly and fixation between the top frame 5, the top cover 4 and the bottom cover 1 are completed, and the cover plate 3 and the microfluidic chip 2 are also positioned, which improves the detection efficiency of circulating tumor cell capture and enhances practicality. By setting the air pressure fixing method, during assembly, the top frame 5, the top cover 4 and the bottom cover 1 can be assembled with uniform force at the fixed point, avoiding the time-consuming and labor-intensive traditional bolt assembly and uneven force problems, and enhancing stability.

[0027] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art.

Claims

1. A circulating tumor cell capture and release chip, comprising a bottom cover (1), a microfluidic chip (2) attached to the upper surface of the bottom cover (1), a cover plate (3) placed on the surface of the microfluidic chip (2), a top cover (4) placed on the surface of the cover plate (3), and a top frame (5) placed on the surface of the top cover (4), characterized in that: The bottom of the top frame (5) is fixedly connected to a top connecting pipe (6), the top cover (4) is provided with a first sealing groove (7), the bottom of the top cover (4) is fixedly connected to a bottom connecting pipe (8), the bottom of the bottom cover (1) is provided with a second sealing groove (9), and an adjustment component (10) is installed at the inner center of the bottom cover (1); The adjustment assembly (10) comprises a shell (101) fixedly connected to the inside of the bottom cover (1); the inside of the shell (101) is sealingly and slidably connected to a sealing plate (102); a threaded groove (103) is provided at the center of the inside of the sealing plate (102); a bolt (104) is meshed and rotatably connected inside the threaded groove (103); a positioning bearing (105) is fixedly installed on the outer surface of the bolt (104); a venting groove (106) is provided on the surface of the shell (101); a connecting pipe (107) is fixedly connected to the surface of the shell (101) away from the bolt (104); one end of the connecting pipe (107) away from the shell (101) is connected to the inside of the second sealing groove (9); a limiting groove (108) is provided inside the sealing plate (102); a limiting rod (109) is inserted into the limiting groove (108).

2. A circulating tumor cell capture and release chip according to claim 1, characterized in that: The top connecting pipe (6) and the bottom connecting pipe (8) are both annular, and sealing rings are fixedly mounted on the outer surfaces of the top connecting pipe (6) and the bottom connecting pipe (8).

3. A circulating tumor cell capture and release chip according to claim 1, characterized in that: The outer surface of the top connecting pipe (6) is sealed and inserted into the interior of the first sealing groove (7), and the outer surface of the bottom connecting pipe (8) is sealed and inserted into the interior of the second sealing groove (9).

4. A circulating tumor cell capture and release chip according to claim 1, characterized in that: One end of the interior of the housing (101) close to the connecting pipe (107) is connected to the interior of the second sealing groove (9) through the connecting pipe (107), and the outer surface of the positioning bearing (105) is fixedly mounted inside the housing (101).

5. A circulating tumor cell capture and release chip according to claim 1, characterized in that: The interior of the housing (101) away from the connecting pipe (107) is connected to the exterior of the housing (101) through the air discharge groove (106).

6. A circulating tumor cell capture and release chip according to claim 1, characterized in that: The number of the limiting rods (109) and the limiting grooves (108) is such that one end of the two limiting rods (109) away from the connecting tube (107) is fixedly connected to the inner wall of the housing (101), and the surface of the microfluidic chip (2) is modified with a nucleic acid aptamer.

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