Purification device for nucleic acid extraction

Abstract

The utility model relates to the technical field of nucleic acid extraction and purification, and discloses a purification device for nucleic acid extraction, which comprises a box body, the top of the box body is rotatably connected with a hollow shaft penetrating through the top wall of the box body, the bottom end of the hollow shaft is provided with a refrigeration mechanism, the refrigeration mechanism comprises a shunt pipe, and the shunt pipe is fixedly connected to the bottom of the hollow shaft. The inner side of the hollow shaft is fixedly connected with a first air guide pipe, the top of the first air guide pipe is fixedly connected with a connector, the top end of the connector is rotationally connected with a second air guide pipe, the inlet end of the second air guide pipe communicates with a refrigerator, and the refrigerator is fixedly installed at the top of the box body; a driving mechanism is arranged at one end of the top of the box. According to the utility model, cold air can be uniformly dispersed to different areas of the box body by the air spraying pipe, so that the cooling speeds of different areas in the box body are consistent, and the temperature control effect is greatly improved.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction and purification, and more specifically, to a purification device for nucleic acid extraction. Background Art

[0002] Nucleic acid extraction and purification are the basis of molecular biology experiments. The method of nucleic acid purification is the most important factor affecting the quality of extracted nucleic acids, and is also the key to the success or failure of downstream molecular biology experiments. Currently, the common methods for nucleic acid purification include PC extraction, high salt precipitation, centrifugal column and biomagnetic beads. These methods each have their own advantages and disadvantages. Generally, high-pressure precipitation nucleic acid extraction and purification devices often do not have the function of temperature control, resulting in the inability of proteins to reach the optimal precipitation temperature, which is easy to form residues, affecting the purity of nucleic acid extraction and cannot meet people's usage needs;

[0003] In this regard, Chinese patent publication number CN210683812U discloses "a nucleic acid extraction and purification device, comprising a box body, the top outer wall of the box body is provided with a protective shell, and the top inner wall of the protective shell is provided with a motor, the outer wall of one end of the motor output shaft is provided with a rotating column, and the outer walls on both sides of the rotating column are provided with stirring rods, the top outer wall of the box body is opened with a fixing hole, and a liquid inlet bucket is inserted into the fixing hole, and the outer wall of one side of the box body is provided with a temperature sensor."

[0004] The above-mentioned device controls the operation of the refrigerator body through the control panel to perform temperature control work inside the box, wherein the cold air in the refrigerator is mainly introduced into the interior of the box through the air duct, which reduces the temperature inside the box, thereby keeping the inside of the box at a low temperature; however, since the air duct is arranged at one end of the box, the cold air first cools down the area where the air duct is located, while other areas require the cold air to diffuse completely before cooling down, which makes the cooling speed inside the box inconsistent, thereby affecting the nucleic acid purification work in different areas. Utility Model Content

[0005] In order to overcome the deficiencies of the prior art, the utility model provides a purification device for nucleic acid extraction, which has the advantage of being able to evenly diffuse cold air to ensure that the cooling speeds of different areas inside the box are consistent.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a purification device for nucleic acid extraction, comprising a box body, the top of the box body is rotatably connected with a hollow shaft that penetrates the top wall of the box body, the bottom end of the hollow shaft is provided with a refrigeration mechanism, the refrigeration mechanism comprises a shunt pipe, the shunt pipe is fixedly connected to the bottom of the hollow shaft, both ends of the bottom of the shunt pipe are fixedly connected with jet pipes that communicate with the inside of the shunt pipe, an air guide pipe 1 is fixedly connected to the inner side of the hollow shaft, the bottom end of the air guide pipe 1 is communicated with the inside of the shunt pipe, the top of the air guide pipe 1 is fixedly connected with a connector, the top of the connector is rotatably connected with an air guide pipe 2, the inlet end of the air guide pipe 2 is connected with a refrigerator, the refrigerator is fixedly installed on the top of the box body, and one end of the top of the box body is provided with a driving mechanism for driving the hollow shaft to rotate.

[0007] As a preferred technical solution of the utility model, the other end of the top of the box body is fixedly connected to a liquid inlet pipe, one end of the bottom of the box body is fixedly connected to a liquid discharge pipe, and a filter is fixedly connected to the pipe mouth of the liquid discharge pipe.

[0008] As a preferred technical solution of the utility model, a sampling tube is fixedly connected to the outer side of the box body, and a control valve is fixedly connected to the sampling tube.

[0009] As a preferred technical solution of the utility model, the driving mechanism includes a motor, which is fixedly installed on the top of the box, the output shaft of the motor is fixedly connected to a driving gear, the top of the hollow shaft is fixedly connected to a gear ring, and the driving gear is meshed with the gear ring.

[0010] As a preferred technical solution of the utility model, the bottom of the diversion pipe is fixedly connected to a rotating shaft, the outer side of the rotating shaft is fixedly connected to a plurality of stirring rods, the plurality of stirring rods are equidistantly distributed along the axial direction of the rotating shaft, and the bottom of the rotating shaft is provided with a cleaning piece for cleaning the top wall of the filter by cooperating with the rotation of the rotating shaft.

[0011] As a preferred technical solution of the utility model, the angle between the stirring rod and the rotating shaft is 30 degrees, and the stirring rods at the same height are distributed around the rotating shaft at equal angles.

[0012] As a preferred technical solution of the utility model, the cleaning piece includes a mounting plate, one end of the mounting plate is fixedly connected to the rotating shaft, the other end of the mounting plate is slidably connected to a sliding rod that passes through the mounting plate, the bottom of the sliding rod is fixedly connected to a brush plate, the brush plate can cooperate with the rotation of the rotating shaft to intermittently interfere with the top wall of the filter, and the top of the sliding rod is fixedly connected to a stopper.

[0013] As a preferred technical solution of the utility model, a spring is sleeved on the slide rod, the top end of the spring abuts against the mounting plate, and the bottom end of the spring abuts against the brush plate.

[0014] Compared with the prior art, the beneficial effects of the utility model are as follows: the air guide pipe can guide the cold air inside the refrigerator into the shunt pipe and spray it out through the jet pipe. The hollow shaft can be rotated by the driving mechanism, which makes the shunt pipe rotate synchronously with the hollow shaft, and all the jet pipes rotate synchronously in the circumferential direction. In this way, the jet pipe can evenly disperse the cold air to different areas of the box, so that the cooling speed of different areas inside the box is consistent, which greatly improves the temperature control effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A schematic diagram of the structure of a purification device for nucleic acid extraction according to the utility model Figure 1 ;

[0016] Figure 2 A schematic diagram of the structure of a purification device for nucleic acid extraction according to the utility model Figure 2 ;

[0017] Figure 3 It is a structural schematic diagram of the refrigeration mechanism of the utility model;

[0018] Figure 4 It is a structural schematic diagram of the driving mechanism of the utility model;

[0019] Figure 5 It is a structural schematic diagram of the cleaning piece of the utility model.

[0020] In the figure: box body 1, hollow shaft 2, rotating shaft 3, stirring rod 4, refrigeration mechanism 5, shunt pipe 51, jet pipe 52, air guide pipe 1 53, connector 54, air guide pipe 2 55, refrigerator 56, driving mechanism 6, motor 61, driving gear 62, gear ring 63, drain pipe 7, filter screen 8, cleaning part 9, mounting plate 91, slide rod 92, brush plate 93, block 94, spring 95, sampling tube 10, control valve 11, liquid inlet pipe 12. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] like Figures 1 to 5As shown, the utility model provides a purification device for nucleic acid extraction, including a box body 1, a liquid inlet pipe 12 is fixedly connected to the other end of the top of the box body 1, a liquid discharge pipe 7 is fixedly connected to one end of the bottom of the box body 1, a filter 8 is fixedly connected to the pipe mouth of the liquid discharge pipe 7, a sampling tube 10 is fixedly connected to the outside of the box body 1, and a control valve 11 is fixedly connected to the sampling tube 10. The filter 8 can prevent protein precipitation from clogging the connecting pipe, and by opening the control valve 11, the sampling tube 10 can lead out part of the nucleic acid solution for sampling and detection, which is very convenient to operate.

[0023] The top of the box body 1 is rotatably connected to a hollow shaft 2 that passes through the top wall of the box body 1, and a refrigeration mechanism 5 is arranged at the bottom end of the hollow shaft 2. The refrigeration mechanism 5 includes a shunt pipe 51, and the shunt pipe 51 is fixedly connected to the bottom of the hollow shaft 2. Both ends of the bottom of the shunt pipe 51 are fixedly connected to injection pipes 52 that communicate with the inside of the shunt pipe 51. An air guide pipe 1 53 is fixedly connected to the inner side of the hollow shaft 2, and the bottom end of the air guide pipe 1 53 is communicated with the inside of the shunt pipe 51. The top of the air guide pipe 1 53 is fixedly connected to a connector 54, and the top of the connector 54 is rotatably connected to an air guide pipe 2 55. The inlet end of the air guide pipe 2 55 is connected to a refrigerator 56, and the refrigerator 56 is fixedly installed on the top of the box body 1. One end of the top of the box body 1 is provided with a driving mechanism 6 for driving the hollow shaft 2 to rotate.

[0024] The air guide pipe 53 can guide the cold air inside the refrigerator 56 into the shunt pipe 51 and spray it out through the jet pipe 52. The hollow shaft 2 can be rotated by the driving mechanism 6, which makes the shunt pipe 51 rotate synchronously with the hollow shaft 2, and all the jet pipes 52 rotate synchronously in the circumferential direction. In this way, the jet pipes 52 can evenly disperse the cold air to different areas of the box 1, so that the cooling speed of different areas inside the box 1 is consistent, which greatly improves the temperature control effect.

[0025] The driving mechanism 6 includes a motor 61, which is fixedly mounted on the top of the housing 1. The output shaft of the motor 61 is fixedly connected to a driving gear 62, and the top of the hollow shaft 2 is fixedly connected to a gear ring 63, and the driving gear 62 is meshed with the gear ring 63. The motor 61 is started to rotate the driving gear 62, and since the driving gear 62 is meshed with the gear ring 63, the gear ring 63 drives the hollow shaft 2 to rotate, which makes the shunt pipe 51 rotate synchronously with the hollow shaft 2, which makes all the jet pipes 52 rotate synchronously, so that the jet pipes 52 can evenly disperse the cold air to different areas of the housing 1.

[0026] Among them, the bottom of the diversion pipe 51 is fixedly connected with the rotating shaft 3, and the outer side of the rotating shaft 3 is fixedly connected with a plurality of stirring rods 4, and the plurality of stirring rods 4 are equidistantly distributed along the axial direction of the rotating shaft 3, and the angle between the stirring rod 4 and the rotating shaft 3 is 30 degrees. The stirring rods 4 at the same height are distributed at equal angles around the rotating shaft 3. A cleaning member 9 is provided at the bottom of the rotating shaft 3 for cleaning the top wall of the filter screen 8 by cooperating with the rotation of the rotating shaft 3. The cleaning member 9 includes a mounting plate 91, one end of the mounting plate 91 is fixedly connected with the rotating shaft 3, and the other end of the mounting plate 91 is slidably connected with a sliding rod 92 that penetrates the mounting plate 91, and a brush plate 93 is fixedly connected to the bottom of the sliding rod 92, and the brush plate 93 can cooperate with the rotation of the rotating shaft 3 to intermittently conflict with the top wall of the filter screen 8, and a stopper 94 is fixedly connected to the top of the sliding rod 92, and a spring 95 is sleeved on the sliding rod 92, and the top end of the spring 95 conflicts with the mounting plate 91, and the bottom end of the spring 95 conflicts with the brush plate 93.

[0027] By starting the motor 61, the driving gear 62 rotates. Since the driving gear 62 is meshed with the ring gear 63, the ring gear 63 will drive the hollow shaft 2 to rotate, which makes the diverter tube 51 rotate synchronously with the hollow shaft 2, and the rotating shaft 3 drives all the stirring rods 4 to rotate synchronously, thereby accelerating the precipitation rate of the protein. At the same time, the mounting plate 91 will drive the brush plate 93 to rotate circumferentially. When the brush plate 93 contacts the top wall of the filter screen 8, the brush plate 93 can clean the protein on the top wall of the filter screen 8, effectively avoiding the problem of the filter holes of the filter screen 8 being blocked.

[0028] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0029] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A purification device for nucleic acid extraction, comprising a housing (1), characterized in that: The top of the box body (1) is rotatably connected to a hollow shaft (2) penetrating the top wall of the box body (1); the bottom end of the hollow shaft (2) is provided with a refrigeration mechanism (5); the refrigeration mechanism (5) comprises a shunt pipe (51); the shunt pipe (51) is fixedly connected to the bottom of the hollow shaft (2); both ends of the bottom of the shunt pipe (51) are fixedly connected to an injection pipe (52) communicating with the inside of the shunt pipe (51); the inner side of the hollow shaft (2) is fixedly connected to an air guide pipe (53); The bottom end of the air guide tube 1 (53) is communicated with the interior of the shunt tube (51); the top of the air guide tube 1 (53) is fixedly connected to a connector (54); the top of the connector (54) is rotatably connected to the air guide tube 2 (55); the inlet end of the air guide tube 2 (55) is connected to a refrigerator (56); the refrigerator (56) is fixedly mounted on the top of the box body (1); and a driving mechanism (6) for driving the hollow shaft (2) to rotate is provided at one end of the top of the box body (1).

2. A purification device for nucleic acid extraction according to claim 1, characterized in that: The other end of the top of the box body (1) is fixedly connected to a liquid inlet pipe (12), one end of the bottom of the box body (1) is fixedly connected to a liquid discharge pipe (7), and a filter screen (8) is fixedly connected to the pipe mouth of the liquid discharge pipe (7).

3. A purification device for nucleic acid extraction according to claim 1, characterized in that: A sampling tube (10) is fixedly connected to the outside of the box body (1), and a control valve (11) is fixedly connected to the sampling tube (10).

4. A purification device for nucleic acid extraction according to claim 1, characterized in that: The driving mechanism (6) comprises a motor (61), the motor (61) is fixedly mounted on the top of the box (1), the output shaft of the motor (61) is fixedly connected to a driving gear (62), the top of the hollow shaft (2) is fixedly connected to a gear ring (63), and the driving gear (62) is meshed with the gear ring (63).

5. A purification device for nucleic acid extraction according to claim 2, characterized in that: The bottom of the diverter pipe (51) is fixedly connected to a rotating shaft (3), and the outer side of the rotating shaft (3) is fixedly connected to a plurality of stirring rods (4), and the plurality of stirring rods (4) are evenly distributed along the axial direction of the rotating shaft (3). The bottom of the rotating shaft (3) is provided with a cleaning member (9) for cleaning the top wall of the filter screen (8) by cooperating with the rotation of the rotating shaft (3).

6. A purification device for nucleic acid extraction according to claim 5, characterized in that: The included angle between the stirring rod (4) and the rotating shaft (3) is 30 degrees, and the stirring rods (4) at the same height are distributed at equal angles around the rotating shaft (3).

7. A purification device for nucleic acid extraction according to claim 5, characterized in that: The cleaning member (9) comprises a mounting plate (91), one end of which is fixedly connected to the rotating shaft (3), the other end of which is slidably connected to a slide bar (92) which passes through the mounting plate (91), the bottom of which is fixedly connected to a brush plate (93), which can cooperate with the rotation of the rotating shaft (3) to intermittently contact the top wall of the filter screen (8), and the top of which is fixedly connected to a stopper (94).

8. A purification device for nucleic acid extraction according to claim 7, characterized in that: The slide bar (92) is sleeved with a spring (95), the top end of the spring (95) abuts against the mounting plate (91), and the bottom end of the spring (95) abuts against the brush plate (93).

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