Single cell sequencing sample processing and crushing device

Abstract

The utility model discloses a single-cell sequencing sample processing and crushing device which comprises a box body, a cavity is formed in the box body, a box door is installed on the box body, a bearing mechanism is arranged in the box body, a container is arranged in the box body, and the bearing mechanism is connected with the container and used for supporting the container. A spline sleeve is rotatably mounted at the top in the box body and is vertically arranged, a spline shaft is inserted in the spline sleeve, the spline shaft is located in the box body, the spline sleeve is in spline fit with the spline shaft, the lower end of the spline shaft extends out of the spline sleeve, a plurality of blades distributed in a circumferential array are fixedly mounted on the spline shaft, and the blades can move into the container; an installation cover is fixedly installed outside the box body, and an electric telescopic rod is fixedly installed in the installation cover.

Description

Technical Field

[0001] The utility model relates to the technical field related to sample processing, and in particular to a single-cell sequencing sample processing and crushing device. Background Art

[0002] The single-cell sequencing sample processing and fragmentation device is a device used to process samples during the single-cell sequencing process. Its main function is to fragment cells so as to extract their genomes or transcriptomes for sequencing. In single-cell sequencing, since the genome or transcriptome of a single cell needs to be sequenced, the cells need to be fragmented and the nucleic acid material inside them needs to be extracted. This fragmentation process requires the use of a special device to ensure that the cells are fully fragmented while maintaining the integrity and sequenceability of the nucleic acid. The specific fragmentation method may vary depending on the purpose of the experiment and the cell type, but generally includes physical fragmentation (such as ultrasonic fragmentation, high-pressure fragmentation, etc.) and chemical fragmentation (such as enzyme digestion, etc.). The choice of these fragmentation methods depends on the type, hardness, structure of the cell, and the specific requirements of the experiment. The specific design and operation of the single-cell sequencing sample processing and fragmentation device may vary from laboratory to laboratory and research project to research project. When using these devices, relevant operating procedures and safety guidelines need to be followed to ensure the accuracy and safety of the experiment. In actual work, the existing single-cell sequencing sample processing and fragmentation devices may directly affect the quality and accuracy of the sequencing results due to incomplete fragmentation. Incomplete cell fragmentation means that the amount of nucleic acid (such as DNA or RNA) that can be extracted will be reduced. This will result in insufficient data available during the sequencing process, which may reduce the coverage and accuracy of the sequencing results. To this end, we propose a single-cell sequencing sample processing and fragmentation device. Utility Model Content

[0003] In order to solve the defects in the prior art, the utility model provides a single-cell sequencing sample processing and fragmentation device.

[0004] In order to solve the above technical problems, the utility model provides the following technical solutions:

[0005] The utility model discloses a single-cell sequencing sample processing and fragmentation device, comprising a box body, a cavity is arranged inside the box body, a box door is installed on the box body, a supporting mechanism is arranged inside the box body, a container is arranged inside the box body, and the supporting mechanism is connected to the container and used to support the container;

[0006] A spline sleeve is rotatably installed on the top of the box body, the spline sleeve is vertically arranged, a spline shaft is inserted in the spline sleeve, the spline shaft is located in the box body, the spline sleeve is splined with the spline shaft, the lower end of the spline shaft extends to the outside of the spline sleeve, a plurality of blades distributed in a circular array are fixedly installed on the spline shaft, the blades can be moved to the inside of the container, a mounting cover is fixedly installed outside the box body, an electric telescopic rod is fixedly installed in the mounting cover, the output end of the electric telescopic rod passes through the box body and extends to the inside of the spline sleeve, the output end of the electric telescopic rod is rotatably installed at the top center of the spline shaft, a driving mechanism is arranged in the box body, the driving mechanism is connected to the spline sleeve and can drive the blades to rotate around the spline shaft.

[0007] As a preferred technical solution of the present utility model, a sealing gasket layer is provided at the connection between the box door and the box body.

[0008] As a preferred technical solution of the utility model, the driving mechanism includes a ring gear, which is located in the housing and fixedly connected to the spline sleeve. A first motor is fixedly installed in the housing, and a driving wheel is fixedly installed on the output shaft end of the first motor, and the driving wheel is meshed with the ring gear.

[0009] As a preferred technical solution of the present utility model, a protective cover is installed on the first motor.

[0010] As a preferred technical solution of the utility model, the supporting mechanism includes an annular frame, which is fixedly connected to the box body, and a support plate is rotatably installed in the annular frame. A carrier plate is provided at the upper end of the carrier plate, and two symmetrically distributed card slots are fixedly installed on the top of the carrier plate. Side blocks are fixedly installed on both sides of the container, and the side blocks are inserted into the card slots.

[0011] As a preferred technical solution of the utility model, an annular groove is fixedly installed on the top of the support plate, and a plurality of spherical parts are fixedly installed on the bottom of the carrier plate, and the spherical parts are slidably arranged inside the annular groove.

[0012] As a preferred technical solution of the present utility model, an anti-corrosion coating is provided inside the container.

[0013] As a preferred technical solution of the utility model, a connecting shaft is provided at the bottom of the carrier plate, a second motor is provided in the annular frame, the second motor is fixedly installed in the box, and the output shaft of the second motor is spline-matched with the connecting shaft.

[0014] The beneficial effects of the utility model are:

[0015] This single-cell sequencing sample processing and fragmentation device drives the sample liquid in the container to rotate. The cells in the sample liquid will be subjected to centrifugal force and shear force. These forces help to rupture the cell membrane, making the nucleic acid material in the cell easier to extract. At the same time, the rotation action also helps to make the cells more evenly distributed, thereby improving the efficiency and effect of the fragmentation. The stirring action of the blades can make the cells in the sample solution evenly distributed, preventing the cells from precipitating or aggregating in the solution. This uniform distribution helps the cells to be subjected to more uniform forces during the fragmentation process, thereby improving the efficiency of cell fragmentation. The improvement in the fragmentation effect helps to release the nucleic acid material in the cell more comprehensively, providing higher quality samples for subsequent sequencing. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0017] Figure 1 This is a schematic diagram of the structure of a single-cell sequencing sample processing and fragmentation device of the utility model;

[0018] Figure 2 This is a schematic diagram of the internal structure of a single-cell sequencing sample processing and fragmentation device of the utility model;

[0019] Figure 3 This is an enlarged cross-sectional view of a portion of the structure of a single-cell sequencing sample processing and fragmentation device of the utility model;

[0020] Figure 4 The utility model is a single cell sequencing sample processing and crushing device Figure 3 An enlarged schematic diagram of some structures in .

[0021] In the figure: box body 1, box door 2, container 3, spline sleeve 4, spline shaft 5, blade 6, mounting cover 7, electric telescopic rod 8, ring gear 9, first motor 10, driving wheel 11, annular frame 12, support plate 13, carrier plate 14, slot 15, side block 16, annular groove 17, spherical part 18, connecting shaft 19, second motor 20. DETAILED DESCRIPTION

[0022] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0023] Example: Figure 1 , Figure 2 , Figure 3 and Figure 4As shown, the utility model is a single-cell sequencing sample processing and fragmentation device, comprising a box body 1, a cavity is arranged inside the box body 1, a box door 2 is installed on the box body 1, a supporting mechanism is arranged inside the box body 1, a container 3 is arranged inside the box body 1, and the supporting mechanism is connected to the container 3 and is used to support the container 3;

[0024] A spline sleeve 4 is rotatably mounted on the top of the box body 1. The spline sleeve 4 is vertically arranged. A spline shaft 5 is inserted in the spline sleeve 4. The spline shaft 5 is located in the box body 1. The spline sleeve 4 is spline-matched with the spline shaft 5. The lower end of the spline shaft 5 extends to the outside of the spline sleeve 4. A plurality of blades 6 distributed in a circumferential array are fixedly mounted on the spline shaft 5. The blades 6 can move to the inside of the container 3. A mounting cover 7 is fixedly mounted outside the box body 1. An electric telescopic rod 8 is fixedly mounted inside the mounting cover 7. The output end of the electric telescopic rod 8 passes through the box body 1 and extends to the inside of the spline sleeve 4. The output end of the electric telescopic rod 8 is rotatably mounted at the top center of the spline shaft 5. A driving mechanism is arranged in the box body 1. The driving mechanism is connected to the spline sleeve 4 and can drive the blades 6 to rotate around the spline shaft 5. A sealing gasket layer is arranged at the connection between the box door 2 and the box body 1.

[0025] The driving mechanism includes a gear ring 9, which is located in the housing 1 and fixedly connected to the spline sleeve 4. A first motor 10 is fixedly installed in the housing 1. A driving wheel 11 is fixedly installed at the output shaft end of the first motor 10, and the driving wheel 11 meshes with the gear ring 9. A protective cover is installed on the first motor 10.

[0026] The supporting mechanism includes an annular frame 12, which is fixedly connected to the box body 1. A support plate 13 is rotatably installed in the annular frame 12. A carrier plate 14 is arranged on the upper end of the support plate 13. Two symmetrically distributed card slots 15 are fixedly installed on the top of the carrier plate 14. Side blocks 16 are fixedly installed on both sides of the container 3, and the side blocks 16 are inserted into the card slots 15. An annular groove 17 is fixedly installed on the top of the support plate 13, and a plurality of spherical parts 18 are fixedly installed on the bottom of the carrier plate 14. The spherical parts 18 are slidably arranged in the annular groove 17. An anti-corrosion coating is arranged inside the container 3. A connecting shaft 19 is arranged at the bottom of the carrier plate 14. A second motor 20 is arranged in the annular frame 12. The second motor 20 is fixedly installed in the box body 1, and the output shaft of the second motor 20 is spline-matched with the connecting shaft 19.

[0027] During operation, the container 3 is filled with sample liquid, and the connecting shaft 19 is driven to rotate by the second motor 20. The connecting shaft 19 rotates synchronously with the container 3, which can drive the sample liquid in the container 3 to rotate continuously during the crushing operation. At the same time, the spline shaft 5 is driven to move by the electric telescopic rod 8, and the blades 6 at the lower end of the spline shaft 5 are all moved into the container 3. Then, the driving wheel 11 is driven to rotate by the first motor 10, and the driving wheel 11 drives the ring gear 9 to rotate. The ring gear 9 and the spline sleeve 4 and the spline shaft 5 rotate synchronously, thereby driving the blades 6 to rotate and stirring the sample solution.

[0028] When the container rotates, the cells in the sample liquid will be subjected to centrifugal force and shear force, which help to rupture the cell membrane, making it easier to extract the nucleic acid material in the cell. At the same time, the rotation action also helps to make the cells more evenly distributed, thereby improving the efficiency and effect of the fragmentation. Stirring can make the cells in the sample solution evenly distributed, preventing the cells from precipitating or aggregating in the solution. This uniform distribution helps the cells to be subjected to more uniform forces during the fragmentation process, thereby improving the efficiency of cell fragmentation. The improvement of the fragmentation effect helps to release the nucleic acid material in the cell more comprehensively, providing higher quality samples for subsequent sequencing.

[0029] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A single-cell sequencing sample processing and fragmentation device, comprising a housing (1), characterized in that: The box body (1) is provided with a cavity inside, the box body (1) is provided with a box door (2), the box body (1) is provided with a supporting mechanism inside, the box body (1) is provided with a container (3), and the supporting mechanism is connected to the container (3) and is used to support the container (3); A spline sleeve (4) is rotatably mounted on the top of the box body (1). The spline sleeve (4) is arranged vertically. A spline shaft (5) is inserted into the spline sleeve (4). The spline shaft (5) is located in the box body (1). The spline sleeve (4) and the spline shaft (5) are spline-matched. The lower end of the spline shaft (5) extends outside the spline sleeve (4). A plurality of blades (6) distributed in a circumferential array are fixedly mounted on the spline shaft (5). The blades (6) can be moved into the container (3). The box (1) is provided with a mounting cover (7) fixedly mounted outside the box (1), an electric telescopic rod (8) fixedly mounted inside the mounting cover (7), an output end of the electric telescopic rod (8) passing through the box (1) and extending into the interior of the spline sleeve (4), the output end of the electric telescopic rod (8) being rotatably mounted at the top center of the spline shaft (5), a driving mechanism being arranged inside the box (1), the driving mechanism being connected to the spline sleeve (4) and being capable of driving the blades (6) to rotate around the spline shaft (5).

2. A single-cell sequencing sample processing and fragmentation device according to claim 1, characterized in that: A sealing cushion layer is provided at the connection between the box door (2) and the box body (1).

3. The single-cell sequencing sample processing and fragmentation device according to claim 1, characterized in that: The driving mechanism comprises a gear ring (9), the gear ring (9) is located in the housing (1) and fixedly connected to the spline sleeve (4), a first motor (10) is fixedly installed in the housing (1), a driving wheel (11) is fixedly installed on the output shaft end of the first motor (10), and the driving wheel (11) is meshed with the gear ring (9).

4. A single-cell sequencing sample processing and fragmentation device according to claim 3, characterized in that: A protective cover is installed on the first motor (10).

5. The single-cell sequencing sample processing and fragmentation device according to claim 4, characterized in that: The supporting mechanism comprises an annular frame (12), the annular frame (12) is fixedly connected in the box body (1), a support plate (13) is rotatably mounted in the annular frame (12), a carrier plate (14) is arranged at the upper end of the support plate (13), two symmetrically distributed card slots (15) are fixedly mounted on the top of the carrier plate (14), and side blocks (16) are fixedly mounted on both sides of the container (3), and the side blocks (16) are inserted into the card slots (15).

6. The single-cell sequencing sample processing and fragmentation device according to claim 5, characterized in that: An annular groove (17) is fixedly mounted on the top of the support plate (13), and a plurality of spherical members (18) are fixedly mounted on the bottom of the carrier plate (14), wherein the spherical members (18) are slidably arranged inside the annular groove (17).

7. The single-cell sequencing sample processing and fragmentation device according to claim 6, characterized in that: The container (3) is provided with an anti-corrosion coating inside.

8. The single-cell sequencing sample processing and fragmentation device according to claim 7, characterized in that: A connecting shaft (19) is arranged at the bottom of the carrier plate (14), a second motor (20) is arranged in the annular frame (12), the second motor (20) is fixedly installed in the box body (1), and an output shaft of the second motor (20) is spline-matched with the connecting shaft (19).

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