Device for producing low-glycosylation glucosyl stevioside

Abstract

The utility model discloses a device for producing low-glycosylation glucosyl stevioside, and relates to the technical field of stevioside production, an inlet of a dissolving tank is respectively communicated with a stevioside RA97 tank, a beta-cyclodextrin tank and a purified water tank through pipelines, an outlet of the dissolving tank is communicated with a reaction tank through a pipeline, and the reaction tank is communicated with the beta-cyclodextrin tank through a pipeline. An inlet of the reaction tank is communicated with an acid liquor tank and a glucosyltransferase tank through pipelines, an outlet of the reaction tank is communicated with an enzymolysis tank through a pipeline, an inlet of the enzymolysis tank is communicated with a saccharifying enzyme tank through a pipeline, an outlet of the enzymolysis tank is communicated with a first concentration tank through a pipeline, and an outlet of the first concentration tank is communicated with a chromatographic column through a pipeline; an inlet of the chromatographic column is communicated with a 85% ethanol solution tank through a pipeline, and an outlet of the chromatographic column is respectively communicated with a first finished product tank, a second finished product tank and a third finished product tank. Separation of glucosyl stevioside mixtures with different grafting numbers is achieved, and the taste of the separated three products is more single and pure.

Description

Technical Field

[0001] The utility model relates to the technical field of stevioside production, in particular to a device for producing low-glycosylated glucose-based stevioside. Background Art

[0002] Glucosyl stevioside is a sweetener rich in sugar. A moderate intake can provide the body with the energy it needs, help strengthen the body and relieve fatigue, and can also reduce calorie intake, which can help with weight loss. Glucosyl stevioside can also affect the secretion of insulin, help regulate blood sugar, maintain blood sugar stability, effectively reduce the risk of diabetes, and can also dilate blood vessels, increase blood volume, and help stabilize blood pressure.

[0003] In the conversion process of glucose-based steviol glycoside (enzyme-modified steviol glycoside), different numbers of glucose groups are attached to stevioside, resulting in the product being a mixture of glucose-based steviol glycosides with different grafting numbers. In addition, the reaction conversion process is not easy to control, and the taste characteristics of the product vary due to the different numbers of grafted glucose groups. How to separate the mixture of glucose-based steviol glycosides with different grafting numbers is currently a difficult problem. Summary of the invention

[0004] The technical problem to be solved by the utility model is: in view of the deficiencies in the prior art, a device for producing low-glycosylated glucose-based stevioside is provided, which can separate a mixture of glucose-based steviosides with different grafting numbers, and the obtained low-glycosylated glucose-based stevioside has a purer taste.

[0005] In order to solve the above technical problems, the technical solution of the utility model is:

[0006] A device for producing low-glycosylated glucosyl stevioside comprises a dissolving tank, wherein the inlet of the dissolving tank is connected to a stevioside RA97 tank, a β-cyclodextrin tank and a purified water tank through pipelines, the outlet of the dissolving tank is connected to a reaction tank through a pipeline, the inlet of the reaction tank is connected to an acid tank and a glucosyltransferase tank through a pipeline, the outlet of the reaction tank is connected to an enzymolysis tank through a pipeline, the inlet of the enzymolysis tank is connected to a saccharifying enzyme tank through a pipeline, the outlet of the enzymolysis tank is connected to a first concentration tank through a pipeline, and the inlet of the second concentration pipeline is connected to an ethanol tank through a pipeline;

[0007] The outlet of the first concentration tank is connected to a chromatographic column through a pipeline, the inlet of the chromatographic column is connected to an 85% ethanol solution tank through a pipeline, the outlet of the chromatographic column is connected to a first analysis liquid tank, a second analysis liquid tank and a third analysis liquid tank through pipelines, respectively, and the first analysis liquid tank, the second analysis liquid tank and the third analysis liquid tank are connected to a first finished product tank, a second finished product tank and a third finished product tank through a first dryer, a second dryer and a third dryer, respectively.

[0008] As an improved technical solution, the outlet of the first concentration tank is connected to a filter through a pipeline, and the liquid phase outlet of the filter is connected to the chromatographic column through a pipeline.

[0009] As an improved technical solution, a first automatic regulating valve is provided on the outlet pipe of the acid liquid tank, a pH sensor is provided in the reaction tank, and the first automatic regulating valve and the pH sensor are interlocked to a control system.

[0010] As an improved technical solution, a first flow sensor is provided on the outlet pipe of the dissolution tank, and a second flow sensor and a second automatic regulating valve are provided on the outlet pipe of the glucosyltransferase tank. The first flow sensor, the second flow sensor and the second automatic regulating valve are interlocked to a control system.

[0011] As an improved technical solution, the outlet of the reaction tank is provided with a third flow sensor, the outlet of the saccharifying enzyme tank is provided with a fourth flow sensor and a third automatic regulating valve, and the third flow sensor, the fourth flow sensor and the third automatic regulating valve are interlocked to the control system.

[0012] As an improved technical solution, the first dryer, the second dryer and the third dryer are all spray dryers.

[0013] Due to the adoption of the above technical solution, the beneficial effects of the utility model are:

[0014] The utility model discloses a device for producing low-glycosylated glucosyl stevioside, comprising a dissolving tank, wherein the inlet of the dissolving tank is connected to a stevioside RA97 tank, a β-cyclodextrin tank and a purified water tank through pipelines, the outlet of the dissolving tank is connected to a reaction tank through pipelines, the inlet of the reaction tank is connected to an acid tank and a glucosyltransferase tank through pipelines, the outlet of the reaction tank is connected to an enzymolysis tank through pipelines, the inlet of the enzymolysis tank is connected to a saccharifying enzyme tank through pipelines, the outlet of the enzymolysis tank is connected to a first concentration tank through pipelines, and the inlet of the second concentration pipeline is connected to an ethanol tank through pipelines; the outlet of the first concentration tank is connected to a chromatographic column through pipelines, the inlet of the chromatographic column is connected to an 85% ethanol solution tank through pipelines, the outlet of the chromatographic column is connected to a first analytical liquid tank, a second analytical liquid tank and a third analytical liquid tank through pipelines, and the first analytical liquid tank, the second analytical liquid tank and the third analytical liquid tank are connected to a first finished product tank, a second finished product tank and a third finished product tank through a first dryer, a second dryer and a third dryer, respectively. The raw materials stevioside RA97 and β-cyclodextrin are added to the dissolution tank, and then an appropriate amount of purified water is added to heat and dissolve. The obtained raw material liquid is transferred to the reaction tank, and acid is added to adjust the pH to about 5.0, and then the glucosyl transferase tank is added. After the reaction, the enzyme is heated to inactivate the enzyme to obtain a reaction solution. After the reaction solution is cooled, saccharifying enzyme is added, and after the reaction, the enzyme is heated to inactivate the enzyme to obtain an enzymolysis solution. The enzymolysis solution is put into the first concentration tank for concentration, and then anhydrous ethanol is added. After standing, the supernatant is collected and passed into the chromatographic column. After gradient analysis of 85% ethanol solution, glucose-based stevioside RA+1G analysis solution, glucose-based stevioside 2G analysis solution, and glucose-based stevioside 3G analysis solution are finally obtained, and glucose-based stevioside RA+1G, 2G and 3G products are obtained after drying, respectively, realizing the separation of glucose-based stevioside mixtures with different grafting numbers. After separation, the taste of the three products is more single and pure.

[0015] The outlet of the first concentration tank of the utility model is connected to a filter through a pipeline, and the liquid phase outlet of the filter is connected to the chromatographic column through a pipeline. The supernatant and the filter residue are separated by the filter, and the filter residue can be used as a raw material for manufacturing fertilizers, thereby improving the economic benefits of the process.

[0016] The outlet pipe of the acid tank is provided with a first automatic regulating valve, the reaction tank is provided with a pH sensor, and the first automatic regulating valve and the pH sensor are interlocked to a control system. The control system adjusts the opening and closing of the first automatic regulating valve according to the value of the pH sensor, thereby controlling the amount of acid added, so that the pH of the material in the reaction tank is within a suitable range.

[0017] The outlet pipe of the dissolving tank is provided with a first flow sensor, the outlet pipe of the glucosyltransferase tank is provided with a second flow sensor and a second automatic regulating valve, and the first flow sensor, the second flow sensor and the second automatic regulating valve are interlocked to a control system. The control system matches the amount of glucosyltransferase added according to the amount of raw material liquid added displayed by the first flow sensor, and controls the amount of glucosyltransferase added through the second automatic regulating valve and the second flow sensor.

[0018] The outlet of the reaction tank is provided with a third flow sensor, the outlet of the saccharifying enzyme tank is provided with a fourth flow sensor and a third automatic regulating valve, and the third flow sensor, the fourth flow sensor and the third automatic regulating valve are interlocked to a control system. The control system matches the amount of saccharifying enzyme added according to the amount of reaction liquid added displayed by the third flow sensor, and controls the amount of saccharifying enzyme added through the third automatic regulating valve and the fourth flow sensor.

[0019] The first dryer, the second dryer and the third dryer are all spray dryers, which have better drying effect and shorter drying time, thereby improving production efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0021] Figure 1 It is a structural schematic diagram of an embodiment of the utility model;

[0022] Among them: 1. dissolution tank; 2. stevioside RA97 tank; 3. β-cyclodextrin tank; 4. purified water tank; 5. reaction tank; 6. acid tank; 7. glucosyltransferase tank; 8. enzymolysis tank; 9. saccharifying enzyme tank; 10. first concentration tank; 11. ethanol tank; 12. chromatographic column; 13. 85% ethanol solution tank; 14. first analytical liquid tank; 15. second analytical liquid tank; 16. third analytical liquid tank; 17. first dryer; 18. second dryer; 19. third dryer; 20. first finished product tank; 21. second finished product tank; 22. third finished product tank; 23. filter; 24. first automatic regulating valve; 25. pH sensor; 26. first flow sensor; 27. second flow sensor; 28. second automatic regulating valve; 29. ​​third flow sensor; 30. fourth flow sensor; 31. third automatic regulating valve. DETAILED DESCRIPTION

[0023] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0024] like Figure 1As shown, a device for producing low-glycosylated glucosyl stevioside comprises a dissolving tank 1, the inlet of the dissolving tank 1 is connected to a stevioside RA97 tank 2, a β-cyclodextrin tank 3 and a purified water tank 4 through pipelines, the outlet of the dissolving tank 1 is connected to a reaction tank 5 through a pipeline, the inlet of the reaction tank 5 is connected to an acid tank 6 and a glucosyltransferase tank 7 through a pipeline, the outlet of the reaction tank 5 is connected to an enzymolysis tank 8 through a pipeline, the inlet of the enzymolysis tank 8 is connected to a saccharifying enzyme tank 9 through a pipeline, the outlet of the enzymolysis tank 8 is connected to a first concentration tank 10 through a pipeline, and the second concentration pipeline is connected to a first concentration tank 11 through a pipeline. The inlet is connected to an ethanol tank 11 through a pipeline; the outlet of the first concentration tank 10 is connected to a chromatographic column 12 through a pipeline, the inlet of the chromatographic column 12 is connected to an 85% ethanol solution tank 13 through a pipeline, and the outlet of the chromatographic column 12 is respectively connected to a first analytical liquid tank 14, a second analytical liquid tank 15 and a third analytical liquid tank 16 through pipelines, and the first analytical liquid tank 14, the second analytical liquid tank 15 and the third analytical liquid tank 16 are respectively connected to a first finished product tank 20, a second finished product tank 21 and a third finished product tank 22 through a first dryer 17, a second dryer 18 and a third dryer 19. The raw materials stevioside RA97 and β-cyclodextrin are added to the dissolution tank 1, and then an appropriate amount of purified water is added to heat and dissolve, and the obtained raw material liquid is transferred to the reaction tank 5, and an acid solution is added to adjust the pH to about 5.0, and then the glucosyltransferase tank 7 is added, and the temperature is raised after the reaction to inactivate the enzyme to obtain a reaction solution. After the reaction solution is cooled, saccharifying enzyme is added, and the temperature is raised after the reaction to inactivate the enzyme to obtain an enzymolysis solution. The enzymatic hydrolyzate is put into the first concentration tank 10 for concentration, and then anhydrous ethanol is added. After standing, the supernatant is collected and passed into the chromatographic column 12, and finally glucose stevioside RA+1G analysis solution, glucose stevioside 2G analysis solution, and glucose stevioside 3G analysis solution are obtained through gradient analysis with 85% ethanol solution. After drying, glucose stevioside RA+1G, 2G and 3G products are obtained respectively, and the separation of the mixture of glucose stevioside with different grafting numbers is realized. After separation, the taste of the three products is more single and pure.

[0025] The outlet of the first concentration tank 10 is connected to a filter 23 through a pipeline, and the liquid phase outlet of the filter 23 is connected to the chromatographic column 12 through a pipeline. The supernatant and the filter residue are separated by the filter 23, wherein the filter residue can be used as a raw material for making fertilizers, thereby improving the economic benefits of the process.

[0026] The outlet pipe of the acid tank 6 is provided with a first automatic regulating valve 24, and the reaction tank 5 is provided with a pH sensor 25. The first automatic regulating valve 24 and the pH sensor 25 are interlocked to a control system. The control system adjusts the opening and closing of the first automatic regulating valve 24 according to the value of the pH sensor 25, thereby controlling the amount of acid added, so that the pH of the material in the reaction tank 5 is within a suitable range.

[0027] The outlet pipe of the dissolving tank 1 is provided with a first flow sensor 26, and the outlet pipe of the glucosyltransferase tank 7 is provided with a second flow sensor 27 and a second automatic regulating valve 28. The first flow sensor 26, the second flow sensor 27 and the second automatic regulating valve 28 are interlocked to the control system. The control system matches the amount of glucosyltransferase added according to the amount of raw material liquid added displayed by the first flow sensor 26, and controls the amount of glucosyltransferase added through the second automatic regulating valve 28 and the second flow sensor 27.

[0028] The outlet of the reaction tank 5 is provided with a third flow sensor 29, and the outlet of the saccharifying enzyme tank 9 is provided with a fourth flow sensor 30 and a third automatic regulating valve 31. The third flow sensor 29, the fourth flow sensor 30 and the third automatic regulating valve 31 are interlocked to the control system. The control system matches the amount of saccharifying enzyme added according to the amount of reaction liquid added displayed by the third flow sensor 29, and controls the amount of saccharifying enzyme added through the third automatic regulating valve 31 and the fourth flow sensor 30.

[0029] The first dryer 17, the second dryer 18 and the third dryer 19 are all spray dryers, which have better drying effect and shorter drying time, thereby improving production efficiency.

[0030] It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the claims attached to this application.

Claims

1. A device for producing low-glycosylated glucosyl steviol glycosides, characterized in that: It comprises a dissolving tank, wherein the inlet of the dissolving tank is connected to a stevioside RA97 tank, a β-cyclodextrin tank and a purified water tank through pipelines, the outlet of the dissolving tank is connected to a reaction tank through a pipeline, the inlet of the reaction tank is connected to an acid tank and a glucosyltransferase tank through a pipeline, the outlet of the reaction tank is connected to an enzymolysis tank through a pipeline, the inlet of the enzymolysis tank is connected to a saccharifying enzyme tank through a pipeline, the outlet of the enzymolysis tank is connected to a first concentration tank through a pipeline, and the inlet of the first concentration tank is connected to an ethanol tank through a pipeline; The outlet of the first concentration tank is connected to a chromatographic column through a pipeline, the inlet of the chromatographic column is connected to an 85% ethanol solution tank through a pipeline, the outlet of the chromatographic column is connected to a first analysis liquid tank, a second analysis liquid tank and a third analysis liquid tank through pipelines, respectively, and the first analysis liquid tank, the second analysis liquid tank and the third analysis liquid tank are connected to a first finished product tank, a second finished product tank and a third finished product tank through a first dryer, a second dryer and a third dryer, respectively.

2. The device for producing low-glycosylated glucosyl steviol glycoside according to claim 1, characterized in that: The outlet of the first concentration tank is connected to a filter through a pipeline, and the liquid phase outlet of the filter is connected to the chromatographic column through a pipeline.

3. The device for producing low-glycosylated glucosyl steviol glycoside according to claim 1, characterized in that: A first automatic regulating valve is arranged on the outlet pipeline of the acid liquid tank, a pH sensor is arranged in the reaction tank, and the first automatic regulating valve and the pH sensor are interlocked to a control system.

4. The device for producing low-glycosylated glucosyl steviol glycoside according to claim 1, characterized in that: A first flow sensor is disposed on the outlet pipe of the dissolution tank, and a second flow sensor and a second automatic regulating valve are disposed on the outlet pipe of the glucosyltransferase tank. The first flow sensor, the second flow sensor and the second automatic regulating valve are interlocked to a control system.

5. The device for producing low-glycosylated glucosyl steviol glycoside according to claim 1, characterized in that: The outlet of the reaction tank is provided with a third flow sensor, the outlet of the saccharifying enzyme tank is provided with a fourth flow sensor and a third automatic regulating valve, and the third flow sensor, the fourth flow sensor and the third automatic regulating valve are interlocked to a control system.

6. The device for producing low-glycosylated glucosyl steviol glycoside according to claim 1, characterized in that: The first dryer, the second dryer and the third dryer are all spray dryers.

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