Single cell capture culture dish

Abstract

The utility model relates to the technical field of medical instruments, in particular to a single cell capture culture dish which comprises a culture dish cover body, a one-way valve and a culture dish main body, a plurality of cell sample adding grooves are formed in the bottom inside the culture dish main body, and a plurality of cell washing grooves are formed in one side of each cell sample adding groove. A plurality of cell distinguishing grooves are formed in the side, away from the cell sample adding groove, of the cell washing groove, two cell capturing grooves are evenly formed in each cell distinguishing groove, and cell culture holes are formed in the centers of the two cell capturing grooves. Compared with the prior art, the problem that environment contrast experiments cannot be carried out in the same culture dish is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, in particular to a single cell capture culture dish. Background Art

[0002] The single-cell capture dish is a biotechnology tool for isolating, capturing and culturing single cells. This technology has a wide range of applications in cell biology, medical research and biomedicine. Traditional sequencing is performed on a multi-cell basis, and what is actually obtained is the average of the signals in a large number of cells, which loses the information of cell heterogeneity (differences between cells). Single-cell sequencing technology can detect heterogeneity information that cannot be obtained by sequencing mixed samples, thus solving this problem well.

[0003] In the prior art, a single-cell capture culture dish in a Chinese patent document with publication number CN219670526U proposes a single-cell capture culture dish, including a dish body, a cell loading groove and a single-cell capture groove are provided in the dish body, the bottom surface of the single-cell capture groove is a concave surface, and a single-cell capture blind hole for accommodating a single cell is provided at the lowest point of the bottom surface of the single-cell capture groove. The single-cell capture culture dish of the utility model has a simple structure and is easy to operate. It can effectively avoid the mixing of multiple cells, the loss and contamination of single cells, and can significantly improve the efficiency of manual single-cell capture. However, consistent with the traditional method, the culture dish in the technical solution presents an integrated shape as a whole, and it is impossible to divide the cell samples into different zones and culture them in different environments, so it is difficult to obtain experimental results under comparison. When it is necessary to carry out culture experiments in different environments, multiple culture dishes need to be operated separately, which is complicated to operate and inefficient and easy to confuse. Utility Model Content

[0004] In view of this, the purpose of the present invention is to provide a single cell capture culture dish to solve the problem that environmental comparison experiments cannot be performed in the same culture dish.

[0005] Based on the above purpose, the utility model provides a single cell capture culture dish, comprising: a culture dish cover, a one-way valve and a culture dish body, wherein a plurality of cell loading grooves are provided at the bottom of the interior of the culture dish body, a plurality of cell washing grooves are provided on one side of the cell loading grooves, a plurality of cell distinguishing grooves are provided on the side of the interior of the culture dish body away from the cell loading grooves, two cell capture grooves are evenly provided inside the cell distinguishing grooves, and cell culture holes are provided at the center positions of the two cell capture grooves.

[0006] Preferably, an air inlet is provided above the culture dish cover, and the air inlet passes through the top surface of the culture dish cover. At the same time, the one-way valve is arranged inside the air inlet and the outer diameter of the one-way valve is consistent with the inner diameter of the air inlet. At the same time, an air relief valve used in conjunction with the one-way valve is provided inside the culture dish body.

[0007] Preferably, two limiting components are arranged at two side positions of the culture dish cover, and a sealing gasket is arranged at an inner position of the culture dish cover.

[0008] Preferably, two baffles are arranged inside the one-way valve, a fixing block is arranged at the bottom of each of the two baffles, and a coil spring is arranged at the middle connecting position of the two baffles.

[0009] Preferably, a partition is provided inside the culture dish body, which evenly divides the interior of the culture dish body into three parts. The internal structures of the three partitions are consistent, and the sealing gasket inside the culture dish cover is distributed correspondingly to the partition inside the culture dish body, and the two are used in conjunction with each other.

[0010] Preferably, a groove is provided on both sides of the limiting assembly, and the position of the groove corresponds to and is used in conjunction with the limiting assembly.

[0011] Beneficial effects of the utility model:

[0012] 1. This single-cell capture culture dish is provided with a partition design, so that when the device is used, it can use different conditions such as the environment to compare and analyze the overall test process, so as to obtain more rigorous experimental data. At the same time, it avoids the need to prepare multiple culture dishes for comparative experiments in the traditional way, saving the cost of using the device.

[0013] 2. This single-cell capture culture dish is provided with a sealing pad and a limiting component so that the environment in each compartment of the device as a whole is guaranteed to be absolutely independent when in use, without interfering with or communicating with each other. The overall humidity and the content of other substances in the air can be adjusted through the opened air inlet, thereby obtaining a more accurate experimental process and experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only for the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] Figure 1It is a schematic diagram of the three-dimensional structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the structure of the culture dish of the utility model;

[0017] Figure 3 This is a schematic diagram of the structure of the culture dish cover of the utility model;

[0018] Figure 4 This is a schematic diagram of the interior of the dish cover of the utility model;

[0019] Figure 5 This is a schematic diagram of the one-way valve structure of the utility model.

[0020] The markings in the figure are:

[0021] 1. Culture dish cover; 2. Air inlet; 3. One-way valve; 4. Limiting assembly; 5. Culture dish body; 6. Cell loading tank; 7. Cell washing tank; 8. Cell separation tank; 9. Cell capture tank; 10. Cell culture hole; 11. Sealing pad; 12. Baffle; 13. Fixing block; 14. Coil spring. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments.

[0023] It should be noted that, unless otherwise defined, the technical terms or scientific terms used in the present invention should be understood by people with ordinary skills in the field to which the present invention belongs. The words "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. "Include" or "comprise" and similar words mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0024] like Figures 1 to 5As shown, a single cell capture culture dish comprises: a culture dish cover 1, a one-way valve 3 and a culture dish body 5, a plurality of cell loading grooves 6 are provided at the bottom of the interior of the culture dish body 5, a plurality of cell washing grooves 7 are provided on one side of the cell loading groove 6, a plurality of cell distinguishing grooves 8 are provided on the side of the culture dish body 5 away from the cell loading groove 6, two cell capturing grooves 9 are evenly provided inside the cell distinguishing groove 8, and a cell culture hole 10 is provided at the center of the two cell capturing grooves 9. This design is to separate and screen the cells layer by layer during the operation and finally release them. The cells are placed in a specific position. The design of the cell differentiation groove 8 is to facilitate the observation of the effects of different culture solutions on cells. The multiple settings make the overall practicability of the device richer and more convenient. The setting of the cell capture groove 9 is to facilitate the placement of cells in a specified position. At the same time, the setting of the cell culture hole 10 makes the position of the cells more fixed and more convenient to observe. During use, the sample is first placed in the cell loading groove 6, and then the cells are taken from the cell loading groove 6 to the cell washing groove 7 for screening, and then a single cell is placed in the cell culture hole 10, and then cultured separately.

[0025] Further, such as Figure 1 and Figure 3 As shown, an air inlet 2 is arranged above the culture dish cover 1, and the air inlet 2 passes through the top surface of the culture dish cover 1. At the same time, a one-way valve 3 is arranged inside the air inlet 2, and the outer diameter of the one-way valve 3 is consistent with the inner diameter of the air inlet 2. This design is to better maintain a more independent environmental system between the three compartments. At the same time, the design of only inlet but not out makes the environment in the separate space more stable, which is conducive to the scientific nature of the experimental data and the improvement of the practical value of the device. During use, the air inlet 2 needs to be connected to an oxygen generator and other equipment first, and then the environment of the interval space is adjusted by the equipment. At the same time, an air relief valve (not shown in the figure) used in conjunction with the one-way valve 3 is arranged inside the culture dish body 5, so that the air pressure inside the device is in a standard air pressure state.

[0026] Further, such as Figure 4 As shown, two limiting components 4 are arranged on the two side positions of the culture dish cover 1, and a sealing pad 11 is arranged on the inner position of the culture dish cover 1. This design allows the culture dish cover 1 to be better fixed to the culture dish body 5 after the cells are sorted and cultured, thereby better ensuring the stability of the environment inside the device, thereby ensuring the integrity of the experimental data inside the device. During use, the culture dish cover 1 is covered on the top of the culture dish body 5, and then the limiting component 4 is moved, so that the limiting component 4 is stuck in the groove opened at the lower side of the culture dish body 5, thereby completing the limiting sealing function.

[0027] Further, such as Figure 5As shown, two baffles 12 are arranged inside the one-way valve 3, and a fixing block 13 is arranged at the bottom of each of the two baffles 12. At the same time, a coil spring 14 is arranged at the middle connecting position of the two baffles 12. Such a design makes the main body of the one-way valve 3 present an effect of only inlet but not outlet, so as to better ensure that the internal environment is more stable and will not easily cause the influencing factors of the external environment to enter the device and affect the experimental data. During use, when oxygen is input into the device through the air inlet 2, the one-way valve 3 opens, and when the input gas is finished, the one-way valve 3 closes.

[0028] Further, such as Figure 2 As shown, a partition is provided inside the culture dish body 5, and the partition evenly divides the interior of the culture dish body 5 into three parts. The internal structures of the three partitioned parts are consistent, and the sealing gasket 11 inside the culture dish cover 1 is distributed correspondingly to the partition inside the culture dish body 5, and the two are used in conjunction with each other. Such a design divides the interior of the device into three independent spaces, which is convenient for better comparative experiments and does not require additional devices, thereby improving the practicality of the device. During use, after the culture dish cover 1 and the culture dish body 5 are combined, three independent sealed spaces appear as a whole, which is convenient for experimental comparison.

[0029] Further, such as Figure 2 As shown, a groove is provided on both sides of the limit component 4, and the position of the groove corresponds to the limit component 4 and is used in conjunction with the limit component 4. This design allows the device to be more sealed and more sturdy through the limit component 4 and the groove after the device cover and the dish body are completed. This avoids imbalance in the internal environment of the device due to inadequate fixing. During use, the movable structure of the limit component 4 can be pressed into the groove.

[0030] Working principle: During use, the culture dish cover 1 and the culture dish body 5 of the device are first separated by the limiting component 4, and then the cultured cells are placed in the cell loading groove 6. The cells are taken from the cell loading groove 6 to the cell washing groove 7 by a tool for cleaning and separation, and the separated single cells are moved to the cell capture groove 9. The cells will then fall into the cell culture hole 10, and different culture fluids are added to the respective cell differentiation grooves 8. After separation is completed, the culture dish cover 1 and the culture dish body 5 are fixed by the limiting component 4, and then different gases are input through the one-way valves 3 of each partition to conduct a comparative experiment.

[0031] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of the present invention (including the claims) is limited to these examples. Under the concept of the present invention, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

[0032] The utility model is intended to cover all such substitutions, modifications and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the utility model should be included in the scope of protection of the utility model.

Claims

1. A single cell capture culture dish, comprising: A culture dish cover (1), a one-way valve (3) and a culture dish body (5), characterized in that a plurality of cell loading grooves (6) are provided at the bottom of the interior of the culture dish body (5), a plurality of cell washing grooves (7) are provided on one side of the cell loading grooves (6), a plurality of cell distinguishing grooves (8) are provided on the interior of the culture dish body (5) away from the cell loading grooves (6), two cell capturing grooves (9) are evenly provided inside the cell distinguishing grooves (8), and cell culture holes (10) are provided at the center of the two cell capturing grooves (9).

2. A single cell capture culture dish according to claim 1, characterized in that: An air inlet (2) is arranged above the culture dish cover (1), and the air inlet (2) penetrates the top surface of the culture dish cover (1). The one-way valve (3) is arranged inside the air inlet (2), and the outer diameter of the one-way valve (3) is consistent with the inner diameter of the air inlet (2). At the same time, an air release valve used in conjunction with the one-way valve (3) is arranged inside the culture dish body (5).

3. The single cell capture culture dish according to claim 1, characterized in that: Two limiting components (4) are arranged at two side positions of the culture dish cover (1), and a sealing pad (11) is arranged at an inner position of the culture dish cover (1).

4. The single cell capture culture dish according to claim 1, characterized in that: Two baffles (12) are arranged inside the one-way valve (3), a fixing block (13) is arranged at the bottom of each of the two baffles (12), and a coil spring (14) is arranged at the middle connecting position of the two baffles (12).

5. The single cell capture culture dish according to claim 3, characterized in that: A partition is provided inside the culture dish body (5), and the partition evenly divides the inside of the culture dish body (5) into three parts. The internal structures of the three partition parts are consistent, and the sealing pad (11) inside the culture dish cover (1) is distributed correspondingly to the partition inside the culture dish body (5).

6. The single cell capture culture dish according to claim 3, characterized in that: A groove is provided on both side surfaces of the limiting component (4), and the position of the groove corresponds to and is used in conjunction with the limiting component (4).

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