Biological stem cell culture dish

Abstract

The utility model relates to the technical field of stem cell culture facilities, and discloses a biological stem cell culture dish which comprises a base, a fixing ring is fixedly connected to the inner wall of the top of the base, containing columns are fixedly connected to the inner walls of the four sides of the fixing ring, and limiting plates are slidably connected to the interiors of the containing columns. A first spring is arranged in the containing column, a sliding rod is fixedly connected to one side of the limiting plate, a clamping block is fixedly connected to one end of the sliding rod, a culture vessel is arranged on the inner wall of the top of the base, a cover plate is rotatably connected to the rear end of the top of the base, and a sealing ring is fixedly connected to the front end of the cover plate. The inner wall of the front end of the cover plate is fixedly connected with a sealing assembly. According to the utility model, the position of the culture vessel is fixed, the non-uniformity of a cell layer caused by the movement or vibration of the culture vessel in the culture process is prevented, and the repeatability and the accuracy of an experimental result are prevented from being influenced.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell culture facilities, in particular to a biological stem cell culture dish. Background Art

[0002] Biological stem cell culture dishes are special containers used to culture and study stem cells in a controlled environment. These culture dishes are usually made of transparent plastic materials with optimized cell attachment surfaces and biocompatibility to support the growth, proliferation and maintenance of stem cells. Their design takes into account the physiological conditions required for cell growth, such as gas exchange, nutrient supply and cell attachment, thus providing a basic and controllable experimental environment for stem cell research.

[0003] Biological stem cell culture dishes are devices specially designed to support and promote the growth, proliferation and maintenance of stem cells. Culture dishes are usually made of transparent plastic or glass and have an optimized cell attachment surface, such as special coatings or treatments, to provide an ideal environment for cell growth. During the culture process, researchers need to regularly replace the culture dishes to maintain suitable growth conditions and observe the morphology and proliferation of cells under a microscope. However, if the culture dishes are not fixed during specific use, the fixed culture dishes may cause uneven cell layers due to movement or vibration during the culture process, resulting in uneven distribution of cells on the surface of the culture dish, affecting the repeatability and accuracy of the experimental results. Therefore, a biological stem cell culture dish is proposed to solve the above problems. Utility Model Content

[0004] In order to make up for the above shortcomings, the utility model provides a biological stem cell culture dish, aiming to improve the problem that the culture dish cannot be fixed in the prior art.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A biological stem cell culture dish, comprising a base, wherein the top inner wall of the base is fixedly connected with a fixing ring, the inner walls of the four sides of the fixing ring are fixedly connected with a containing column, the interior of the containing column is slidably connected with a limiting plate, a spring is arranged inside the containing column, a sliding rod is fixedly connected with one side of the limiting plate, a clamping block is fixedly connected with one end of the sliding rod, a culture vessel is arranged on the top inner wall of the base, a cover plate is rotatably connected with the top rear end of the base, a sealing ring is fixedly connected with the front end of the cover plate, a sealing assembly is fixedly connected with the front inner wall of the cover plate, mounting assemblies are slidably connected with the left and right sides of the base, and a card block is fixedly connected with both sides of the front end of the cover plate, and a card slot is provided inside the card block;

[0007] As a further description of the above technical solution:

[0008] The sealing assembly comprises a fixed rod, the left and right ends of the fixed rod are fixedly connected to the inner walls of the front end of the cover plate, the two ends of the fixed rod are slidably connected to sliding blocks, the outer sleeve of the fixed rod is provided with a second spring, one side of the sliding block is rotatably connected to a rotating rod, and one end of the two rotating rods is rotatably connected to a sealing gasket;

[0009] As a further description of the above technical solution:

[0010] Each of the mounting components includes a pull rod, the outside of the pull rod is slidably connected to the inner walls of the left and right sides of the base, the outside of the pull rod is sleeved with a spring three, the outside of the pull rod is fixedly connected with a limiting plate, the top left and right sides of the base are provided with grooves, the grooves are engaged with the clamping blocks, and the pull rod is engaged with the clamping slots;

[0011] As a further description of the above technical solution:

[0012] The outer parts of the two limiting plates are respectively slidably connected to the inner walls on both sides of the top of the base, and the outer part of the sliding rod is slidably connected to the inner wall of one side of the accommodating column;

[0013] As a further description of the above technical solution:

[0014] One side of the two sliding blocks is slidably connected to the front end of the cover plate, and the outside of the sealing gasket is slidably connected to the inner wall of the sealing ring;

[0015] As a further description of the above technical solution:

[0016] The front side of the sealing gasket is in contact with the top of the fixing ring;

[0017] As a further description of the above technical solution:

[0018] One end of the spring 1 is fixedly connected to one side of the limit plate, and the other end of the spring 1 is fixedly connected to the inner wall of one side of the accommodating column;

[0019] As a further description of the above technical solution:

[0020] A handle is fixedly connected to the top end of the rear side of the cover plate, and support blocks are fixedly connected to the four corners of the bottom of the base.

[0021] The utility model has the following beneficial effects:

[0022] 1. In the utility model, by placing the culture vessel on the top inner wall of the base and then pushing the clamping block, the clamping block will drive the sliding rod to move, the sliding rod will drive the limiting plate to move, and the limiting plate will squeeze the spring 1. Under the action of the spring 1, the position of the culture vessel is fixed to prevent the culture vessel from moving or vibrating during the culture process, resulting in uneven cell layers, so that the cells are evenly distributed on the surface of the culture vessel, avoiding affecting the repeatability and accuracy of the experimental results.

[0023] 2. In the utility model, by pulling the pull rod and then covering the cover plate, and then releasing the pull rod, the pull rod will engage with the slot, and at the same time the fixing ring will squeeze the sealing gasket, and the sealing gasket will drive the sliding block to move, thereby squeezing the spring 2, thereby sealing the top of the culture vessel, which can effectively prevent external microorganisms and other contaminants from entering the culture environment, reduce the risk of contamination in cell culture, and significantly reduce the evaporation of water in the culture medium, which helps to maintain the nutrients in the culture medium. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a three-dimensional schematic diagram of a biological stem cell culture dish proposed by the utility model;

[0025] Figure 2 This is a schematic diagram of the structure of a restriction plate of a biological stem cell culture dish proposed by the utility model;

[0026] Figure 3 This is a structural schematic diagram of a sliding block of a biological stem cell culture dish proposed by the utility model;

[0027] Figure 4 The present invention is a schematic structural diagram of a sliding rod of a biological stem cell culture dish.

[0028] Legend:

[0029] 1. Base; 2. Fixed ring; 3. Accommodating column; 4. Limiting plate; 5. Spring 1; 6. Sliding rod; 7. Clamping block; 8. Culture vessel; 9. Cover plate; 10. Sealing ring; 11. Fixed rod; 12. Sliding block; 13. Spring 2; 14. Rotating rod; 15. Sealing pad; 16. Pull rod; 17. Limiting plate; 18. Spring 3; 19. Groove; 20. Block; 21. Slot. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0031] Reference Figure 1 , Figure 3 and Figure 4 The utility model provides an embodiment: a biological stem cell culture dish, including a base 1, the base 1 provides a stable support foundation to support the structure and function of the entire device, the top inner wall of the base 1 is fixedly connected to a fixing ring 2, the four inner walls of the fixing ring 2 are fixedly connected to a receiving column 3, the interior of the receiving column 3 is slidably connected to a limiting plate 4, the interior of the receiving column 3 is provided with a spring 5, one side of the limiting plate 4 is fixedly connected to a sliding rod 6, the limiting plate 4 is mainly used to limit the position of the sliding rod 6 to ensure that the device can accurately control the movement range of the sliding rod 6 during operation, and the sliding rod 6 One end of the base 1 is fixedly connected with a clamping block 7, and the top inner wall of the base 1 is provided with a culture vessel 8, which is the main container for accommodating biological stem cells or bacterial cultures. The top rear end of the base 1 is rotatably connected with a cover plate 9, and the front end of the cover plate 9 is fixedly connected with a sealing ring 10. The function of the sealing ring 10 is to ensure the sealing performance of the cover plate 9 and the base 1 to prevent the internal environment of the culture dish from being polluted by the outside world or the gas exchange from being disturbed. The front inner wall of the cover plate 9 is fixedly connected with a sealing component, which further enhances the sealing performance of the culture dish and ensures the stability and purity of the cell environment during the culture process;

[0032] The sealing assembly includes a fixed rod 11, the left and right ends of the fixed rod 11 are fixedly connected to the inner walls on both sides of the front end of the cover plate 9, and both ends of the fixed rod 11 are slidably connected to sliding blocks 12. The design allows the sliding block 12 to move along the axis of the fixed rod 11 when necessary to adjust or maintain the position and tightening force of the sealing assembly. The outer sleeve of the fixed rod 11 is provided with a spring 13. The function of the spring 13 is to provide appropriate elastic support to ensure that the sealing assembly maintains uniform pressure in the closed state to prevent air or contaminants from entering the culture dish. One side of the sliding block 12 is rotatably connected to a rotating rod 14, and one end of the two rotating rods 14 is rotatably connected to a sealing pad 15. The left and right sides of the base 1 are slidably connected to mounting assemblies.

[0033] Reference Figure 1 - Figure 3Each mounting assembly includes a pull rod 16, the outside of which is slidably connected to the inner walls of the left and right sides of the base 1, and the outside of the pull rod 16 is sleeved with a spring three 18. The function of the spring three 18 is to provide the necessary rebound force to ensure that the pull rod 16 can automatically return to the correct position after the pressure is released, thereby maintaining the functional integrity of the sealing assembly. Blocks 20 are fixedly connected to both sides of the front end of the cover plate 9. The function of the block 20 is to ensure the firm connection between the cover plate 9 and the mounting assembly to prevent loosening or incomplete sealing during use. A slot 21 is provided inside the block 20, and a limiting plate 17 is fixedly connected to the outside of the pull rod 16. Grooves 19 are provided on the left and right sides of the top of the base 1, and the grooves 19 are engaged with the block 20, and the pull rod 16 is engaged with the slot 21.

[0034] Reference Figure 2 - Figure 4 The outer parts of the two limiting plates 17 are respectively slidably connected to the inner walls on both sides of the top of the base 1, the outer part of the sliding rod 6 is slidably connected to the inner wall of one side of the accommodating column 3, one side of the two sliding blocks 12 is slidably connected to the front end of the cover plate 9, and the outer part of the sealing gasket 15 is slidably connected to the inner wall of the sealing ring 10. The sliding connection design of the sealing gasket 15 enables it to fit tightly with the sealing ring 10 to prevent leakage of liquid or gas and maintain a stable sealing state during operation;

[0035] The front side of the sealing gasket 15 fits with the top of the fixing ring 2, one end of the spring 5 is fixedly connected to one side of the limit plate 4, and the other end of the spring 5 is fixedly connected to the inner wall of one side of the accommodating column 3. A handle is fixedly connected to the top end of the rear side of the cover plate 9. The design of the handle facilitates the operator to open or close the cover plate 9 when needed, making the operation more convenient and safer. Support blocks are fixedly connected to the four corners of the bottom of the base 1. The function of the support blocks is to enhance the stability and balance of the base 1 to prevent it from moving or tilting due to vibration or load during use.

[0036] Working principle: when the device needs to be used, the culture vessel 8 is placed on the top of the base 1, and the clamping block 7 is pushed. The clamping block 7 will drive the sliding rod 6 to move, and the sliding rod 6 will drive the limiting plate 4 to move. The limiting plate 4 will squeeze the spring 15, and the spring 15 will generate opposite elastic potential energy. Then, the culture vessel 8 will be fixed under the action of the spring 15 to prevent the culture vessel 8 from moving or vibrating during the culture process. Then, the pulling rod 16 is pulled, and the pulling rod 16 will drive the limiting plate 17, and the limiting plate 17 will squeeze the spring 3 18. Then, put on the cover plate 9 to make the block 20 engage with the inner wall of the groove 19. Then, release the pull rod 16. Under the action of the spring three 18, the outer part of the pull rod 16 will engage with the inner wall of the groove 21. At the same time, the fixed ring 2 will squeeze the sealing gasket 15, and the sealing gasket 15 will drive the rotating rod 14 to rotate. The rotating rod 14 will drive the sliding block 12 to move. The sliding block 12 will squeeze the spring two 13. Under the action of the spring two 13, the front side of the sealing gasket 15 will fit with the top of the fixed ring 2, thereby achieving a sealing effect.

[0037] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A biological stem cell culture dish, comprising a base (1), characterized in that: The top inner wall of the base (1) is fixedly connected to a fixing ring (2), the four side inner walls of the fixing ring (2) are fixedly connected to a containing column (3), the interior of the containing column (3) is slidably connected to a limit plate (4), a spring (5) is arranged inside the containing column (3), one side of the limit plate (4) is fixedly connected to a sliding rod (6), one end of the sliding rod (6) is fixedly connected to a clamping block (7), a culture vessel (8) is arranged on the top inner wall of the base (1), the top rear end of the base (1) is rotatably connected to a cover plate (9), the front end of the cover plate (9) is fixedly connected to a sealing ring (10), the front inner wall of the cover plate (9) is fixedly connected to a sealing component, the left and right sides of the base (1) are slidably connected to mounting components, the front sides of the cover plate (9) are fixedly connected to a clamping block (20), and a clamping groove (21) is provided inside the clamping block (20).

2. A biological stem cell culture dish according to claim 1, characterized in that: The sealing assembly comprises a fixed rod (11), the left and right ends of the fixed rod (11) are fixedly connected to the inner walls on both sides of the front end of the cover plate (9), both ends of the fixed rod (11) are slidably connected to sliding blocks (12), the outer portion of the fixed rod (11) is sleeved with a second spring (13), one side of the sliding block (12) is rotatably connected to a rotating rod (14), and one end of the two rotating rods (14) is rotatably connected to a sealing pad (15).

3. A biological stem cell culture dish according to claim 1, characterized in that: Each of the mounting components comprises a pulling rod (16), the outside of the pulling rod (16) is slidably connected to the inner walls on the left and right sides of the base (1), the outside of the pulling rod (16) is sleeved with a spring three (18), the outside of the pulling rod (16) is fixedly connected with a limiting plate (17), and grooves (19) are provided on the left and right sides of the top of the base (1), the grooves (19) are engaged with the clamping block (20), and the pulling rod (16) is engaged with the clamping slot (21).

4. A biological stem cell culture dish according to claim 3, characterized in that: The exterior of the two limiting plates (17) are respectively slidably connected to the inner walls on both sides of the top of the base (1), and the exterior of the sliding rod (6) is slidably connected to the inner wall of one side of the accommodating column (3).

5. The biological stem cell culture dish according to claim 2, characterized in that: One side of the two sliding blocks (12) is slidably connected to the front end of the cover plate (9), and the outside of the sealing gasket (15) is slidably connected to the inner wall of the sealing ring (10).

6. A biological stem cell culture dish according to claim 2, characterized in that: The front side of the sealing gasket (15) is in contact with the top of the fixing ring (2).

7. The biological stem cell culture dish according to claim 1, characterized in that: One end of the spring 1 (5) is fixedly connected to one side of the limiting plate (4), and the other end of the spring 1 (5) is fixedly connected to the inner wall of one side of the containing column (3).

8. The biological stem cell culture dish according to claim 1, characterized in that: A handle is fixedly connected to the top end of the rear side of the cover plate (9), and support blocks are fixedly connected to the four corners of the bottom of the base (1).

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